Standard name
Human Ortholog
Description Myosin-like protein associated with the nuclear envelope; nuclear basket protein that connects the nuclear pore complex with the nuclear interior; involved with Tel1p in telomere length control; involved with Pml1p and Pml39p in nuclear retention of unspliced mRNAs; MLP1 has a paralog, MLP2, that arose from the whole genome duplication
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Micrographs

ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 – – – – – –
Cell Periphery 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cytoplasm 0 0 0 0 0 0 – 0 0 0 0.05 0 0 0.11 0.15 0.09 0.06 0.11 0 0 0 0 0 0
Endoplasmic Reticulum 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0.05 0 0 0 0 0 0
Endosome 0 0.05 0 0 0.07 0 – 0 0 0 0 0 0 0.06 0.1 0.08 0.08 0.09 0.09 0.13 0.14 0.14 0.15 0.17
Golgi 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Mitochondria 0.35 0.18 0.82 0.61 0.44 0.63 – 0.49 0.66 0.57 0.65 0.59 0.31 0.15 0.14 0.08 0.15 0.07 0 0 0 0.07 0.07 0.12
Nucleus 0.08 0.12 0.15 0.05 0.05 0.06 – 0.06 0.05 0.06 0.07 0.05 0.09 0 0 0.05 0 0.07 0 0 0.05 0 0 0
Nuclear Periphery 0.6 0.52 0.45 0.56 0.56 0.44 – 0.26 0.34 0.27 0.3 0.24 0.57 0.55 0.55 0.55 0.46 0.45 0.67 0.51 0.61 0.49 0.49 0.44
Nucleolus 0.43 0.5 0.21 0.41 0.36 0.32 – 0.4 0.41 0.41 0.42 0.41 0.35 0.35 0.4 0.39 0.44 0.38 0.09 0.23 0.07 0.13 0.1 0.09
Peroxisomes 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 – 0.05 0 0 0 0.05 0 0 0 0.05 0 0 0.05 0 0 0.06 0.06 0
Vac/Vac Membrane 0.08 0.09 0.05 0 0 0 – 0 0 0 0 0 0.05 0.07 0 0.12 0.1 0.14 0 0 0 0 0 0
ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 1 0 – 1 0 0 0 0 1 1 0 0 0 0 0 0 0 0 3 4
Bud 0 0 1 0 3 1 – 15 5 10 9 15 1 0 0 0 0 1 0 1 0 1 3 5
Bud Neck 0 0 0 0 0 0 – 0 0 0 0 3 0 0 0 0 0 0 0 0 0 0 1 1
Bud Site 2 0 0 0 0 5 – 23 4 10 4 15 1 2 2 0 0 2 – – – – – –
Cell Periphery 0 0 0 0 0 0 – 0 0 0 0 0 0 2 0 0 1 0 0 0 0 0 0 0
Cytoplasm 1 2 1 8 9 7 – 22 12 15 19 18 6 41 64 11 10 17 0 1 0 0 0 2
Endoplasmic Reticulum 0 0 0 2 3 4 – 2 2 2 0 1 0 0 11 4 2 7 0 0 0 0 0 0
Endosome 4 3 0 6 33 10 – 22 7 12 10 7 5 21 45 10 12 14 22 11 15 11 56 86
Golgi 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 1 0 0 0 0 4 9
Mitochondria 78 12 79 192 215 261 – 341 322 325 227 377 65 55 63 10 23 11 8 1 4 5 28 60
Nucleus 18 8 14 16 26 26 – 45 24 32 25 34 20 16 7 6 5 10 7 2 5 2 11 10
Nuclear Periphery 133 34 43 178 272 182 – 183 168 155 105 155 120 200 241 69 72 68 171 45 70 38 185 229
Nucleolus 95 33 20 129 175 135 – 279 201 234 145 259 75 127 176 49 69 58 22 20 8 10 38 48
Peroxisomes 0 0 0 0 3 2 – 22 3 4 2 10 1 5 4 0 3 1 2 0 1 0 5 9
SpindlePole 2 0 1 2 7 7 – 32 15 19 5 30 3 3 15 6 4 4 12 1 3 4 21 22
Vac/Vac Membrane 17 6 5 7 21 8 – 11 5 4 6 8 11 27 14 15 15 21 4 3 1 3 14 22
Unique Cell Count 220 66 96 316 489 416 696 489 567 347 634 212 363 438 125 156 151 256 89 114 80 380 519
Labelled Cell Count 350 98 164 540 768 648 998 768 822 557 932 309 500 642 180 216 215 256 89 114 80 380 519


Nuclear Periphery

(Huh et al. Nature 2003 425:686)
Screen WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 AF100 AF140 AF180
Mean Cell GFP Intensity (1e-4) 8.3 9.9 4.8 5.9 6.3 5.4 – 5.7 4.9 5.0 4.8 4.9 8.6 9.7 9.4 12.6 11.8 11.5 7.3 8.6 9.4
Std Deviation (1e-4) 1.7 1.9 0.9 1.6 1.9 1.8 – 2.9 2.0 1.6 1.6 1.6 2.3 3.4 3.8 3.8 3.7 4.2 1.5 2.7 3.1
Intensity Change (Log2) – – – 0.32 0.41 0.18 – 0.25 0.04 0.07 0.01 0.03 0.84 1.02 0.98 1.4 1.31 1.27 0.62 0.86 0.98


Localization RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3
Cortical Patches 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0
Bud 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0
Cell Periphery 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0
Cytoplasm 0 0 0 – 0 0 0 0 0 0 3.1 3.7 2.5 0 3.0
Endoplasmic Reticulum 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0
Endosome 0 2.6 0 – 0 0 0 0 0 0 0 3.3 0 0 3.1
Golgi 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0
Mitochondria -3.9 -6.9 -3.7 – -6.1 -3.2 -4.6 0 0 -8.4 -12.9 -13.6 -11.2 -10.6 -11.9
Nucleus -3.1 -3.3 -2.7 – -2.8 -3.5 -3.2 -2.3 -3.4 -1.3 -3.6 0 -2.5 -3.3 -2.1
Nuclear Periphery 2.0 1.9 -0.2 – -3.8 -1.9 -3.5 0 0 1.9 1.8 1.8 1.5 0.2 0
Nucleolus 3.6 2.8 2.2 – 3.6 3.7 3.8 0 0 2.6 2.6 3.6 2.9 3.8 2.9
Peroxisomes 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0
Vacuole 0 0 0 – 0 0 0 0 0 0 0.8 0 1.7 1.3 2.2

Images

Circle plot not available

Protein Concentration and Protein Localization Data

R1 R2 R3
G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase
Concentration 7.9792 7.6973 7.2326 6.7467 6.8548 7.0246 5.8656 6.7618 5.91 5.5363 5.6728 6.0342 7.9216 8.8325 7.5412 7.6019 6.4717 7.3402
Actin 0.0026 0.0005 0.0066 0.0015 0.0004 0.0002 0.0167 0.0005 0.0115 0.0242 0.0016 0.0071 0.0283 0.0005 0.0172 0.0067 0.0171 0.0004
Bud 0 0 0.0001 0 0 0 0.0002 0 0.0005 0.0001 0.0001 0 0.0004 0.0001 0.0002 0.0002 0.0001 0
Bud Neck 0.0001 0.0001 0.0001 0.0001 0.0001 0.0001 0.0002 0.0001 0.0003 0.0004 0.0007 0.0001 0.0008 0.0001 0.0001 0.0002 0.0001 0.0001
Bud Periphery 0.0001 0.0001 0.0002 0.0001 0.0001 0.0001 0.0004 0.0001 0.0007 0.0003 0.0001 0.0002 0.0007 0.0002 0.0003 0.0004 0.0003 0.0001
Bud Site 0.0002 0.0001 0.0001 0.0001 0 0 0.0007 0.0001 0.0037 0.0002 0.0009 0 0.003 0.0003 0.0004 0.0007 0.0002 0
Cell Periphery 0.0001 0.0001 0 0 0 0 0.0002 0 0.0001 0.0001 0.0001 0.0001 0.0005 0 0.0001 0.0001 0.0001 0
Cytoplasm 0.0001 0.0001 0.0001 0.0001 0 0 0.0054 0.0001 0.0019 0.0002 0.0003 0 0.0091 0.0001 0.0011 0.0022 0.0001 0
Cytoplasmic Foci 0.0017 0.0002 0.0007 0.0008 0.0006 0.0003 0.0126 0.0004 0.0075 0.0032 0.0112 0.0005 0.022 0.0002 0.0025 0.0405 0.0063 0.0003
Eisosomes 0.0002 0 0.0001 0 0 0 0.0002 0 0.0002 0.0001 0 0.0001 0.0004 0 0.0001 0.0001 0.0003 0
Endoplasmic Reticulum 0.0003 0.0002 0.0002 0.0003 0.0001 0.0001 0.0028 0.0001 0.0009 0.0004 0.0004 0.0002 0.0119 0.0003 0.0042 0.0028 0.0013 0.0001
Endosome 0.0011 0.0003 0.0005 0.0009 0.0005 0.0003 0.0152 0.0003 0.0086 0.0027 0.0191 0.0006 0.0317 0.0006 0.0185 0.0412 0.0179 0.0003
Golgi 0.0012 0.0003 0.0007 0.0008 0.0008 0.0005 0.0059 0.0005 0.0046 0.007 0.0086 0.0011 0.0271 0.0005 0.0069 0.0167 0.046 0.0006
Lipid Particles 0.0274 0.0045 0.0158 0.0096 0.0263 0.0116 0.0507 0.0061 0.0161 0.0543 0.0632 0.0207 0.0467 0.0078 0.0082 0.038 0.2262 0.0276
Mitochondria 0.002 0.0009 0.0006 0.0006 0.001 0.0005 0.0108 0.0003 0.0109 0.0024 0.0017 0.002 0.0198 0.0013 0.0044 0.0044 0.0486 0.0009
None 0.0005 0.0002 0.0003 0.0005 0.0002 0.0001 0.0096 0.0002 0.001 0.0004 0.0003 0.0002 0.0043 0.0002 0.0006 0.0009 0.0006 0.0002
Nuclear Periphery 0.8556 0.8392 0.8739 0.8316 0.8429 0.8637 0.7698 0.9017 0.8134 0.7221 0.8098 0.8828 0.6723 0.8083 0.8205 0.734 0.5663 0.9048
Nucleolus 0.0348 0.046 0.0218 0.0725 0.0656 0.0503 0.0217 0.018 0.0274 0.0347 0.0212 0.0252 0.0248 0.0571 0.0186 0.0087 0.0029 0.0254
Nucleus 0.0419 0.0679 0.0392 0.0216 0.0194 0.0381 0.0212 0.0299 0.0333 0.0699 0.0058 0.0272 0.0241 0.0878 0.0498 0.0098 0.0016 0.0185
Peroxisomes 0.0023 0.0001 0.0003 0.0004 0.0002 0.0001 0.0034 0.0003 0.0068 0.0008 0.005 0.0002 0.0371 0.0001 0.0015 0.0088 0.0076 0.0001
Punctate Nuclear 0.0161 0.0274 0.0263 0.0483 0.0318 0.0267 0.0339 0.0311 0.0375 0.0589 0.0393 0.0221 0.0181 0.0134 0.03 0.065 0.0436 0.0112
Vacuole 0.0004 0.0006 0.0003 0.0003 0.0002 0.0003 0.0026 0.0002 0.0009 0.0009 0.0014 0.0007 0.0033 0.0004 0.0006 0.0008 0.0007 0.0002
Vacuole Periphery 0.0111 0.0113 0.0121 0.0098 0.0097 0.0069 0.016 0.0099 0.0122 0.0168 0.0091 0.0091 0.0136 0.0205 0.0142 0.0177 0.0121 0.0092

Sequencing Data

R1 R2
G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Post-START S/G2 Metaphase Anaphase Telophase
Gene Expression 34.5059 35.0525 31.8628 40.1157 37.748 37.426 40.5362 43.5953 39.8003 43.5591
Translational Efficiency 0.7421 0.7234 0.682 0.5961 0.5601 0.677 0.5411 0.5049 0.5302 0.5162

Hit Data

Dataset Hit
Protein Concentration ✘
Protein Localization ✘
Gene Expression ✘
Translational Efficiency ✘
Standard name
Human Ortholog
Description Myosin-like protein associated with the nuclear envelope; nuclear basket protein that connects the nuclear pore complex with the nuclear interior; involved with Tel1p in telomere length control; involved with Pml1p and Pml39p in nuclear retention of unspliced mRNAs; MLP1 has a paralog, MLP2, that arose from the whole genome duplication
Localization
Cell Percentages nuclear periphery (86%)
Cell Cycle Regulation No
Subcompartmental Group N/A

Mlp1

Mlp1


Nearest neighbours ( genes)

correlation threshold

0.6

1.0

0.80


Biological Process (Gene Ontology)
Molecular Function (Gene Ontology)
Cellular Component (Gene Ontology)

Endocytosis
Temp Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Vph1-GFP)
37℃
RT
Cell Cycle Omics

CYCLoPs (Mlp1-GFP)
Gene / Allele Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Sac6-tdTomato)
Gene Images
Gene Images
Images are not yet available
Images are not yet available