Standard name | |
---|---|
Human Ortholog | |
Description | Transmembrane protein with a role in cell wall polymer composition; localizes on inner surface of plasma membrane at bud and in daughter cell; SKG1 has a paralog, AIM20, that arose from the whole genome duplication |
ensLOC | DeepLoc | |||||||||||||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Localization | WT1 | WT2 | WT3 | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 | WT1 | WT2 | WT3 | AF100 | AF140 | AF180 |
Cortical Patches | 50 | 49 | 30 | 12 | 10 | 6 | 25 | – | 18 | 33 | 10 | 18 | 70 | 77 | 52 | 9 | 4 | 5 | 31 | 31 | 13 | 20 | 40 | 32 |
Bud | 8 | 23 | 13 | 5 | 1 | 14 | 14 | – | 22 | 29 | 16 | 23 | 22 | 11 | 11 | 1 | 0 | 0 | 5 | 4 | 1 | 1 | 4 | 5 |
Bud Neck | 0 | 1 | 0 | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 1 | 1 | 0 | 2 | 0 | 0 | 0 | 25 | 43 | 15 | 19 | 24 | 32 |
Bud Site | 6 | 0 | 2 | 0 | 1 | 1 | 0 | – | 0 | 0 | 0 | 1 | 5 | 1 | 0 | 0 | 0 | 0 | – | – | – | – | – | – |
Cell Periphery | 64 | 64 | 45 | 16 | 14 | 4 | 27 | – | 27 | 29 | 21 | 18 | 148 | 187 | 224 | 6 | 6 | 8 | 43 | 23 | 26 | 62 | 108 | 62 |
Cytoplasm | 77 | 89 | 59 | 107 | 141 | 171 | 275 | – | 321 | 312 | 176 | 201 | 55 | 126 | 207 | 28 | 11 | 33 | 7 | 20 | 7 | 6 | 6 | 16 |
Endoplasmic Reticulum | 72 | 41 | 16 | 22 | 16 | 8 | 9 | – | 6 | 6 | 3 | 2 | 4 | 4 | 10 | 6 | 0 | 0 | 91 | 71 | 36 | 45 | 124 | 129 |
Endosome | 3 | 2 | 1 | 1 | 0 | 1 | 0 | – | 0 | 0 | 0 | 0 | 0 | 1 | 0 | 0 | 1 | 1 | 5 | 3 | 4 | 2 | 2 | 4 |
Golgi | 1 | 1 | 0 | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 0 | 1 | 4 | 1 | 0 | 0 | 2 | 1 |
Mitochondria | 6 | 3 | 0 | 0 | 0 | 4 | 0 | – | 0 | 1 | 0 | 0 | 0 | 0 | 1 | 4 | 1 | 5 | 2 | 5 | 2 | 4 | 8 | 6 |
Nucleus | 1 | 1 | 0 | 0 | 4 | 8 | 2 | – | 2 | 3 | 2 | 8 | 2 | 0 | 0 | 16 | 1 | 4 | 0 | 0 | 0 | 0 | 0 | 2 |
Nuclear Periphery | 0 | 0 | 0 | 0 | 0 | 3 | 0 | – | 0 | 0 | 0 | 0 | 0 | 1 | 0 | 0 | 0 | 0 | 1 | 1 | 2 | 0 | 1 | 1 |
Nucleolus | 1 | 0 | 0 | 1 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 1 | 0 | 0 | 0 | 0 | 1 | 0 | 0 | 0 | 0 | 0 |
Peroxisomes | 1 | 0 | 0 | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 2 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
SpindlePole | 1 | 0 | 4 | 3 | 0 | 1 | 0 | – | 3 | 0 | 2 | 0 | 10 | 11 | 11 | 1 | 0 | 1 | 1 | 0 | 0 | 0 | 0 | 0 |
Vac/Vac Membrane | 24 | 3 | 1 | 6 | 9 | 9 | 3 | – | 5 | 11 | 6 | 7 | 0 | 0 | 1 | 45 | 12 | 24 | 84 | 90 | 72 | 30 | 69 | 73 |
Unique Cell Count | 240 | 220 | 138 | 146 | 177 | 198 | 304 | 336 | 353 | 192 | 226 | 215 | 301 | 371 | 93 | 33 | 64 | 311 | 298 | 186 | 197 | 400 | 375 | |
Labelled Cell Count | 315 | 277 | 171 | 173 | 196 | 230 | 355 | 404 | 424 | 236 | 279 | 317 | 420 | 521 | 117 | 36 | 82 | 311 | 298 | 186 | 197 | 400 | 375 |
Screen | WT1 | WT2 | WT3 | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 | AF100 | AF140 | AF180 |
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Mean Cell GFP Intensity (1e-4) | 8.8 | 8.3 | 8.3 | 9.1 | 9.1 | 6.1 | 7.5 | – | 7.0 | 7.0 | 7.1 | 7.1 | 8.4 | 8.6 | 8.5 | 11.2 | 11.9 | 10.5 | 7.6 | 7.9 | 8.0 |
Std Deviation (1e-4) | 1.3 | 1.0 | 1.1 | 5.5 | 1.5 | 1.3 | 1.5 | – | 1.3 | 1.2 | 1.3 | 1.2 | 1.4 | 1.5 | 1.5 | 1.8 | 2.0 | 2.0 | 1.2 | 1.3 | 1.7 |
Intensity Change (Log2) | – | – | – | 0.13 | 0.13 | -0.44 | -0.15 | – | -0.25 | -0.26 | -0.22 | -0.23 | 0.01 | 0.04 | 0.04 | 0.43 | 0.52 | 0.34 | -0.14 | -0.08 | -0.06 |
Localization | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 |
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Cortical Patches | -3.2 | -4.3 | -5.5 | -4.0 | – | -5.4 | -3.7 | -4.5 | -3.8 | 2.2 | 0.9 | -2.1 | -2.4 | -1.2 | -2.4 |
Bud | -2.1 | -3.8 | -0.8 | -2.0 | – | -1.1 | -0.4 | -0.3 | 0.2 | 0.2 | -2.5 | -3.1 | -2.6 | 0 | 0 |
Bud Neck | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Bud Site | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Cell Periphery | -4.4 | -5.6 | -7.8 | -6.3 | – | -6.8 | -6.8 | -4.9 | -6.0 | 6.7 | 5.8 | 5.6 | -4.7 | -1.6 | -3.0 |
Cytoplasm | 5.2 | 6.8 | 8.5 | 10.8 | – | 13.1 | 10.6 | 9.7 | 9.5 | -3.4 | -0.2 | 2.6 | -1.9 | -1.0 | 1.2 |
Endoplasmic Reticulum | 0.9 | -0.7 | -2.6 | -3.6 | – | -4.6 | -4.8 | -3.9 | -4.6 | -3.9 | -4.8 | -4.1 | -1.3 | 0 | -2.8 |
Endosome | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Golgi | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Mitochondria | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Nucleus | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 5.1 | 0 | 0 |
Nuclear Periphery | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Nucleolus | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Peroxisomes | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
SpindlePole | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0.8 | 0 | 0 | 0 | 0 | 0 |
Vacuole | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 8.9 | 0 | 7.4 |
R1 | R2 | R3 | ||||||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | |
Concentration | 3.7861 | 3.8479 | 3.8603 | 4.0367 | 3.9141 | 3.9654 | 6.5072 | 6.5862 | 6.633 | 5.967 | 6.3272 | 6.8163 | 8.7213 | 8.9515 | 9.0122 | 9.1116 | 8.68 | 9.0989 |
Actin | 0.0527 | 0.0096 | 0.0414 | 0.0699 | 0.0267 | 0.0187 | 0.0388 | 0.0091 | 0.0215 | 0.0056 | 0.025 | 0.0133 | 0.0293 | 0.0117 | 0.0301 | 0.0123 | 0.0102 | 0.01 |
Bud | 0.0131 | 0.0052 | 0.0554 | 0.0281 | 0.0279 | 0.0069 | 0.0083 | 0.0126 | 0.0501 | 0.0234 | 0.0201 | 0.0078 | 0.007 | 0.0054 | 0.029 | 0.0106 | 0.0264 | 0.0024 |
Bud Neck | 0.0125 | 0.0147 | 0.0355 | 0.0085 | 0.0528 | 0.4355 | 0.0264 | 0.0183 | 0.0573 | 0.033 | 0.0286 | 0.3536 | 0.0268 | 0.027 | 0.0697 | 0.0197 | 0.1188 | 0.3734 |
Bud Periphery | 0.0514 | 0.0165 | 0.294 | 0.2906 | 0.2618 | 0.0501 | 0.0487 | 0.0433 | 0.3021 | 0.3838 | 0.2645 | 0.0556 | 0.0527 | 0.0197 | 0.2429 | 0.1785 | 0.2578 | 0.0326 |
Bud Site | 0.0511 | 0.3129 | 0.1527 | 0.0144 | 0.0252 | 0.0101 | 0.1196 | 0.3819 | 0.1457 | 0.0209 | 0.0202 | 0.0097 | 0.0881 | 0.3613 | 0.1654 | 0.0057 | 0.0156 | 0.0058 |
Cell Periphery | 0.3114 | 0.1736 | 0.1318 | 0.1866 | 0.1933 | 0.1439 | 0.351 | 0.1925 | 0.1783 | 0.2637 | 0.4257 | 0.2695 | 0.464 | 0.2484 | 0.2352 | 0.4084 | 0.3822 | 0.2885 |
Cytoplasm | 0.2724 | 0.3182 | 0.1324 | 0.2698 | 0.2126 | 0.207 | 0.2199 | 0.2574 | 0.1553 | 0.1237 | 0.1039 | 0.1934 | 0.2151 | 0.2202 | 0.1122 | 0.2342 | 0.1091 | 0.2042 |
Cytoplasmic Foci | 0.0193 | 0.008 | 0.0081 | 0.0078 | 0.0031 | 0.0065 | 0.0207 | 0.0032 | 0.0037 | 0.0022 | 0.0054 | 0.0037 | 0.0034 | 0.0023 | 0.004 | 0.0017 | 0.0011 | 0.0027 |
Eisosomes | 0.0016 | 0.0005 | 0.0008 | 0.0005 | 0.0007 | 0.0003 | 0.0009 | 0.0003 | 0.0003 | 0.0002 | 0.0003 | 0.0005 | 0.0028 | 0.0006 | 0.0005 | 0.0004 | 0.0004 | 0.0003 |
Endoplasmic Reticulum | 0.0354 | 0.0114 | 0.0092 | 0.0101 | 0.0063 | 0.0093 | 0.0238 | 0.0103 | 0.0064 | 0.0265 | 0.009 | 0.0141 | 0.025 | 0.0089 | 0.0117 | 0.0134 | 0.0158 | 0.0123 |
Endosome | 0.038 | 0.0141 | 0.0104 | 0.0072 | 0.0325 | 0.0139 | 0.0298 | 0.0026 | 0.0069 | 0.0072 | 0.0127 | 0.0062 | 0.0023 | 0.0026 | 0.0026 | 0.0025 | 0.002 | 0.0014 |
Golgi | 0.0204 | 0.0098 | 0.0099 | 0.0108 | 0.002 | 0.0107 | 0.0138 | 0.0012 | 0.003 | 0.001 | 0.012 | 0.0034 | 0.0024 | 0.0013 | 0.0017 | 0.0007 | 0.0009 | 0.0011 |
Lipid Particles | 0.0256 | 0.0171 | 0.0192 | 0.0073 | 0.006 | 0.0101 | 0.0159 | 0.009 | 0.0086 | 0.0114 | 0.0041 | 0.0067 | 0.0273 | 0.0134 | 0.0134 | 0.006 | 0.0089 | 0.014 |
Mitochondria | 0.0117 | 0.0009 | 0.0052 | 0.0095 | 0.0019 | 0.0054 | 0.0073 | 0.0002 | 0.001 | 0.0004 | 0.0322 | 0.0009 | 0.0002 | 0.0001 | 0.0003 | 0.0001 | 0.0005 | 0.0002 |
None | 0.0024 | 0.002 | 0.0049 | 0.0011 | 0.0018 | 0.0016 | 0.0084 | 0.0009 | 0.0013 | 0.001 | 0.0004 | 0.0027 | 0.0016 | 0.0007 | 0.0041 | 0.001 | 0.0005 | 0.0028 |
Nuclear Periphery | 0.0133 | 0.0026 | 0.0027 | 0.0072 | 0.0338 | 0.0012 | 0.0058 | 0.0011 | 0.0012 | 0.0024 | 0.0009 | 0.0021 | 0.0016 | 0.0008 | 0.0009 | 0.001 | 0.0011 | 0.0005 |
Nucleolus | 0.0006 | 0.0008 | 0.0011 | 0.0004 | 0.0024 | 0.0007 | 0.0011 | 0.0008 | 0.0009 | 0.0006 | 0.0003 | 0.0007 | 0.0005 | 0.0012 | 0.0009 | 0.0004 | 0.0007 | 0.0005 |
Nucleus | 0.0031 | 0.0018 | 0.0027 | 0.0047 | 0.0161 | 0.0019 | 0.0028 | 0.0024 | 0.0023 | 0.002 | 0.0008 | 0.0024 | 0.001 | 0.0023 | 0.0022 | 0.0011 | 0.0016 | 0.0012 |
Peroxisomes | 0.008 | 0.0004 | 0.0046 | 0.0299 | 0.0003 | 0.0016 | 0.0095 | 0.0001 | 0.0015 | 0.0001 | 0.0026 | 0.0051 | 0.0001 | 0.0001 | 0.0002 | 0 | 0.0001 | 0.0001 |
Punctate Nuclear | 0.001 | 0.0003 | 0.0044 | 0.0008 | 0.0058 | 0.0018 | 0.0054 | 0.0003 | 0.0016 | 0.0002 | 0.0004 | 0.0014 | 0.0002 | 0.0002 | 0.0005 | 0.0001 | 0.0001 | 0.0002 |
Vacuole | 0.0519 | 0.0784 | 0.0714 | 0.0329 | 0.0826 | 0.0614 | 0.0404 | 0.0518 | 0.0499 | 0.0883 | 0.0299 | 0.0465 | 0.0483 | 0.0715 | 0.0717 | 0.1005 | 0.0436 | 0.0452 |
Vacuole Periphery | 0.0032 | 0.0012 | 0.0024 | 0.0021 | 0.0044 | 0.0013 | 0.0017 | 0.001 | 0.0011 | 0.0025 | 0.0011 | 0.0008 | 0.0004 | 0.0004 | 0.0009 | 0.0014 | 0.0027 | 0.0004 |
R1 | R2 | |||||||||
---|---|---|---|---|---|---|---|---|---|---|
G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | |
Gene Expression | 19.1133 | 24.9365 | 21.9306 | 29.5166 | 22.7719 | 19.619 | 24.9798 | 20.1686 | 22.3717 | 28.6721 |
Translational Efficiency | 1.5855 | 0.8919 | 0.9488 | 0.7448 | 1.0207 | 1.4687 | 0.881 | 1.0476 | 1.0411 | 1.0399 |
Dataset | Hit |
---|---|
Protein Concentration | ✔ |
Protein Localization | ✘ |
Gene Expression | ✘ |
Translational Efficiency | ✘ |
R1 | R2 | R1 & R2 | |||||||||
---|---|---|---|---|---|---|---|---|---|---|---|
WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 |
1743 | 964 | 164 | 1858 | 721 | 1537 | 2419 | 1358 | 2464 | 2501 | 2583 | 3216 |
R1 | R2 | R1 & R2 | ||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|
WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 | |
Mean | 897.55 | 944.84 | 1067.28 | 1030.71 | 746.38 | 977.49 | 999.69 | 1080.85 | 853.32 | 964.91 | 1003.98 | 1051.88 |
Standard Deviation | 105.77 | 153.00 | 122.84 | 144.65 | 126.33 | 592.78 | 123.01 | 158.41 | 131.58 | 474.58 | 124.10 | 152.64 |
Intensity Change Log 2 | — | 0.074078 | 0.249875 | 0.199574 | — | 0.389172 | 0.421570 | 0.534184 | — | 0.225707 | 0.330369 | 0.361160 |
R1 | R2 | R1 & R2 | ||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|
WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 | |
Actin | 0.010492 | 0.015019 | 0.102847* | 0.055474 | 0.012676 | 0.013696 | 0.063259 | 0.036715 | 0.011131 | 0.014206 | 0.065772 | 0.047553 |
Bud Neck | 0.146960 | 0.083890 | 0.096574 | 0.088364 | 0.125687 | 0.132906 | 0.167291 | 0.089113 | 0.140735 | 0.114013 | 0.162801 | 0.088680 |
Bud Site | 0.166824* | 0.267164* | 0.356186* | 0.356643* | 0.163468* | 0.222993* | 0.344870* | 0.474934* | 0.165842* | 0.240019* | 0.345588* | 0.406593* |
Cell Periphery | 0.112261* | 0.130281* | 0.130655* | 0.152401* | 0.073163 | 0.137574* | 0.131160* | 0.080265 | 0.100820* | 0.134763* | 0.131128* | 0.121940* |
Cytoplasm | 0.396831* | 0.314924* | 0.087935 | 0.245047* | 0.362570* | 0.333573* | 0.221426* | 0.209086* | 0.386806* | 0.326385* | 0.212950* | 0.229862* |
Cytoplasmic Foci | 0.011474 | 0.043841 | 0.024359 | 0.009091 | 0.067432 | 0.018262 | 0.002577 | 0.016679 | 0.027848 | 0.028121 | 0.003960 | 0.012295 |
Eisosomes | 0.004730 | 0.002419 | 0.008568 | 0.006161 | 0.007830 | 0.005272 | 0.008494 | 0.002583 | 0.005637 | 0.004173 | 0.008498 | 0.004650 |
Endoplasmic Reticulum | 0.011437 | 0.019295 | 0.053192 | 0.003442 | 0.012644 | 0.015855 | 0.005941 | 0.004124 | 0.011790 | 0.017181 | 0.008941 | 0.003730 |
Endosome | 0.012238 | 0.030778 | 0.010613 | 0.007379 | 0.042739 | 0.015856 | 0.001510 | 0.005031 | 0.021163 | 0.021608 | 0.002088 | 0.006387 |
Golgi | 0.004235 | 0.011647 | 0.007958 | 0.005215 | 0.016243 | 0.008680 | 0.000930 | 0.001759 | 0.007749 | 0.009823 | 0.001376 | 0.003756 |
Lipid Particles | 0.005470 | 0.009389 | 0.009409 | 0.006433 | 0.024370 | 0.007126 | 0.002992 | 0.004797 | 0.011000 | 0.007998 | 0.003400 | 0.005742 |
Mitochondria | 0.002234 | 0.001750 | 0.000153 | 0.002102 | 0.002700 | 0.005078 | 0.000910 | 0.000439 | 0.002371 | 0.003795 | 0.000862 | 0.001400 |
Mitotic Spindle | 0.000259 | 0.000893 | 0.000139 | 0.003086 | 0.000118 | 0.000374 | 0.000250 | 0.000193 | 0.000217 | 0.000574 | 0.000243 | 0.001865 |
None | 0.000033 | 0.000063 | 0.000009 | 0.000087 | 0.001638 | 0.000241 | 0.000383 | 0.000097 | 0.000503 | 0.000172 | 0.000359 | 0.000091 |
Nuclear Periphery | 0.000069 | 0.000053 | 0.000079 | 0.000090 | 0.000161 | 0.000054 | 0.000035 | 0.000024 | 0.000096 | 0.000054 | 0.000038 | 0.000062 |
Nuclear Periphery Foci | 0.000389 | 0.000756 | 0.004048 | 0.000532 | 0.001447 | 0.000339 | 0.000317 | 0.000772 | 0.000699 | 0.000500 | 0.000554 | 0.000633 |
Nucleolus | 0.000175 | 0.000094 | 0.000017 | 0.000054 | 0.000235 | 0.000122 | 0.000043 | 0.000042 | 0.000192 | 0.000111 | 0.000041 | 0.000049 |
Nucleus | 0.002045 | 0.001331 | 0.005038 | 0.003839 | 0.003223 | 0.003172 | 0.006307 | 0.003776 | 0.002389 | 0.002463 | 0.006227 | 0.003812 |
Peroxisomes | 0.003957 | 0.005184 | 0.001694 | 0.002923 | 0.006544 | 0.005242 | 0.001434 | 0.002326 | 0.004714 | 0.005220 | 0.001451 | 0.002671 |
Vacuole | 0.104993 | 0.056533 | 0.100141 | 0.049359 | 0.072066 | 0.070284 | 0.039574 | 0.066866 | 0.095358 | 0.064984 | 0.043420 | 0.056752 |
Vacuole Periphery | 0.002894 | 0.004696 | 0.000387 | 0.002278 | 0.003048 | 0.003298 | 0.000296 | 0.000379 | 0.002939 | 0.003837 | 0.000302 | 0.001476 |
R1 | R2 | R1 & R2 | |||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
UBP2_WT | UBP14_WT | UBP2UBP14_WT | UBP2UBP14_UBP2 | UBP2UBP14_UBP14 | UBP2_WT | UBP14_WT | UBP2UBP14_WT | UBP2UBP14_UBP2 | UBP2UBP14_UBP14 | UBP2_WT | UBP14_WT | UBP2UBP14_WT | UBP2UBP14_UBP2 | UBP2UBP14_UBP14 | |
Actin | -3.12 | -7.56 | -17.79 | -14.82 | 3.79 | -0.55 | -19.52 | -9.21 | -10.18 | 9.38 | -2.98 | -25.04 | -20.29 | -18.32 | 7.04 |
Bud Neck | 9.92 | 4.34 | 10.50 | -0.54 | 0.71 | -0.89 | -5.17 | 4.83 | 7.32 | 13.78 | 5.23 | -3.86 | 11.58 | 6.33 | 16.04 |
Bud Site | -9.89 | -8.49 | -21.65 | -7.70 | 0.05 | -5.65 | -18.05 | -25.03 | -22.41 | -12.02 | -10.85 | -25.88 | -33.29 | -21.28 | -7.48 |
Cell Periphery | -2.32 | -1.20 | -5.43 | -2.20 | -1.48 | -8.88 | -8.88 | -0.84 | 8.65 | 8.72 | -6.59 | -6.05 | -3.78 | 2.74 | 2.03 |
Cytoplasm | 7.78 | 26.73 | 17.20 | 6.66 | -14.32 | 2.48 | 13.57 | 13.51 | 13.25 | 1.42 | 8.00 | 25.35 | 22.64 | 13.86 | -3.14 |
Cytoplasmic Foci | -9.18 | -2.82 | 1.29 | 9.87 | 3.24 | 9.92 | 13.66 | 9.94 | 0.41 | -8.35 | -0.10 | 13.77 | 7.86 | 8.31 | -8.76 |
Eisosomes | 5.64 | -4.05 | -2.56 | -7.12 | 2.47 | 2.53 | -0.63 | 5.56 | 6.38 | 12.69 | 3.38 | -5.31 | 2.45 | -1.10 | 8.06 |
Endoplasmic Reticulum | -3.11 | -4.56 | 7.23 | 6.79 | 5.46 | -1.40 | 3.73 | 4.80 | 7.23 | 2.43 | -3.43 | 2.35 | 8.57 | 10.09 | 6.04 |
Endosome | -6.62 | 0.59 | 4.10 | 8.37 | 1.15 | 7.93 | 12.91 | 11.60 | 8.28 | -5.89 | -0.26 | 16.38 | 11.66 | 11.09 | -7.51 |
Golgi | -6.15 | -1.82 | -0.92 | 4.41 | 1.25 | 4.46 | 10.78 | 10.18 | 6.98 | -2.31 | -2.34 | 11.64 | 5.57 | 6.67 | -3.89 |
Lipid Particles | -3.61 | -2.61 | -0.91 | 2.08 | 1.69 | 5.57 | 7.06 | 6.23 | 1.83 | -2.14 | 2.65 | 7.33 | 4.37 | 2.32 | -3.12 |
Mitochondria | 1.64 | 8.82 | 0.41 | -0.96 | -6.46 | -2.83 | 3.94 | 4.88 | 6.50 | 3.74 | -2.91 | 6.93 | 3.59 | 5.06 | -2.83 |
Mitotic Spindle | -0.89 | 0.87 | -3.34 | -2.01 | -3.51 | -2.19 | -0.91 | -2.15 | 1.44 | 0.35 | -1.19 | -0.15 | -3.35 | -2.33 | -3.24 |
None | -0.96 | 2.08 | -1.77 | -0.68 | -2.91 | 1.97 | 1.63 | 2.21 | 0.95 | 0.89 | 1.49 | 0.38 | 2.00 | 0.86 | 0.90 |
Nuclear Periphery | 0.72 | -0.60 | -0.87 | -2.09 | -0.48 | 2.38 | 2.78 | 2.99 | 7.72 | 3.77 | 2.11 | 2.88 | 1.38 | -1.05 | -2.48 |
Nuclear Periphery Foci | -1.71 | -6.66 | -1.16 | 0.97 | 6.37 | 2.33 | 2.36 | 1.23 | -2.96 | -3.05 | 1.15 | 0.80 | 0.18 | -1.40 | -0.95 |
Nucleolus | 1.37 | 2.49 | 1.86 | 3.23 | -5.66 | 2.78 | 4.97 | 4.93 | 5.70 | -0.01 | 1.80 | 3.22 | 2.98 | 6.04 | -2.28 |
Nucleus | 3.41 | -3.26 | -6.76 | -10.73 | 0.95 | 0.04 | -5.52 | -2.16 | -1.29 | 3.77 | -0.14 | -7.53 | -6.94 | -3.89 | 4.00 |
Peroxisomes | -2.24 | 5.91 | 1.79 | 3.39 | -2.41 | 1.78 | 8.16 | 6.19 | 5.97 | -2.98 | -1.22 | 10.99 | 4.94 | 5.98 | -3.98 |
Vacuole | 9.61 | -0.16 | 10.17 | 0.29 | 4.24 | 0.32 | 6.77 | -0.09 | -0.42 | -7.00 | 7.63 | 13.61 | 8.45 | 0.70 | -5.55 |
Vacuole Periphery | -3.16 | 9.48 | 1.12 | 3.27 | -3.37 | -0.52 | 9.80 | 9.39 | 7.22 | -1.34 | -2.47 | 14.87 | 4.17 | 5.35 | -3.72 |
Temp | Actin Patch (Sac6-tdTomato) | Cortical Patch (Sla1-GFP) | Late Endosome (Snf7-GFP) | Vacuole (Vph1-GFP) |
---|---|---|---|---|
37℃ | ||||
RT |
Gene / Allele | Actin Patch (Sac6-tdTomato) | Cortical Patch (Sla1-GFP) | Late Endosome (Snf7-GFP) | Vacuole (Sac6-tdTomato) |
---|
Gene | Images |
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Gene | Images |
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