Standard name
Human Ortholog
Description Protein of unknown function; has weak similarity to E. coli GTP-binding protein gtp1; the authentic, non-tagged protein is detected in highly purified mitochondria in high-throughput studies
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Micrographs

ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud 0 0 0 0.06 0 0 0.06 0 0.07 0.05 0 0.06 0 0 0 0 0.09 0.05 0 0 0 0 0 0.05
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 – – – – – –
Cell Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0.05 0 0 0 0 0 0
Cytoplasm 0.69 0.78 0.66 0.68 0.6 0.47 0.44 0.41 0.38 0.36 0.27 0.32 0.79 0.79 0.78 0.21 0.13 0.14 0.39 0.38 0.12 0.38 0.4 0.38
Endoplasmic Reticulum 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0.07 0.05 0 0 0 0 0 0 0
Endosome 0 0 0 0 0.07 0 0 0 0 0 0 0 0 0 0 0.23 0.3 0.29 0.06 0.06 0.06 0 0 0
Golgi 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Mitochondria 0.49 0.15 0.25 0.32 0.38 0.59 0.68 0.58 0.7 0.74 0.8 0.79 0.05 0 0 0.21 0.07 0.18 0.43 0.38 0.65 0.27 0.25 0.17
Nucleus 0 0 0 0 0 0 0 0 0 0 0 0 0.1 0.15 0.16 0 0.05 0 0 0 0 0.2 0.13 0.18
Nuclear Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0.06 0.08 0.05 0 0 0 0 0 0 0 0 0 0 0.13 0.21 0.24 0 0 0 0 0 0
SpindlePole 0 0 0 0.05 0 0.05 0 0.1 0.07 0 0.07 0.07 0 0 0 0.27 0.22 0.2 0 0 0 0 0 0
Vac/Vac Membrane 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0.1 0.22 0.16 0 0 0 0 0 0.07
ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 3 1 0 2 2 5 3 4 6
Bud 2 0 1 8 3 5 18 12 22 13 5 11 0 1 1 7 10 8 0 1 2 1 11 13
Bud Neck 1 0 2 0 0 2 3 2 2 0 1 0 0 0 2 0 3 0 0 1 0 1 2 5
Bud Site 0 0 0 0 0 1 1 0 8 2 1 3 0 0 0 7 3 4 – – – – – –
Cell Periphery 0 0 4 3 0 0 3 0 2 1 1 1 2 0 0 7 3 7 1 1 0 0 1 2
Cytoplasm 107 102 65 96 101 92 127 128 117 97 43 62 203 197 229 39 15 21 65 57 16 44 103 112
Endoplasmic Reticulum 0 4 0 0 0 1 1 1 1 0 0 0 0 0 0 13 6 3 2 1 2 0 0 3
Endosome 0 5 3 4 11 2 2 11 1 0 0 1 7 2 3 43 34 44 9 9 7 0 4 3
Golgi 0 1 0 0 0 0 0 0 0 0 0 0 0 0 0 7 3 3 1 3 1 0 0 1
Mitochondria 76 20 25 45 63 117 197 184 218 201 129 151 13 1 1 40 8 27 72 57 87 31 65 50
Nucleus 0 0 0 0 1 0 1 1 3 7 1 1 26 37 48 1 6 4 0 0 0 23 33 52
Nuclear Periphery 0 2 0 0 0 1 0 0 1 0 0 0 0 1 0 1 0 0 0 0 0 0 0 0
Nucleolus 0 1 0 0 0 0 1 1 2 3 1 0 2 1 0 2 0 2 0 0 0 2 3 7
Peroxisomes 0 0 6 11 8 1 4 3 2 1 1 1 7 3 4 24 24 36 0 1 2 0 0 0
SpindlePole 0 1 4 7 5 9 12 33 21 10 12 14 6 4 2 51 25 31 3 4 3 3 9 12
Vac/Vac Membrane 2 5 2 1 4 5 3 5 3 4 1 4 7 7 12 19 25 25 3 5 2 4 11 22
Unique Cell Count 155 131 99 141 167 197 291 315 311 273 162 192 258 250 295 190 113 153 168 151 135 118 259 299
Labelled Cell Count 188 141 112 175 196 236 373 381 403 339 196 249 273 254 302 264 166 215 168 151 135 118 259 299


Mitochondria

(Huh et al. Nature 2003 425:686)
Screen WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 AF100 AF140 AF180
Mean Cell GFP Intensity (1e-4) 2.9 4.1 4.2 3.7 4.0 3.0 3.1 3.2 3.0 2.9 2.7 2.6 8.3 9.7 11.7 6.3 6.1 6.5 10.2 9.4 13.4
Std Deviation (1e-4) 0.5 0.8 1.7 2.0 2.0 1.0 1.3 1.5 1.2 0.8 1.8 0.6 9.5 11.2 13.2 1.8 1.5 2.0 11.2 9.8 18.3
Intensity Change (Log2) – – – -0.2 -0.1 -0.48 -0.46 -0.41 -0.5 -0.56 -0.63 -0.68 0.98 1.2 1.47 0.57 0.53 0.62 1.27 1.16 1.66


Localization RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3
Actin – – – – – – – – – – – – – – –
Bud – – – – – – – – – – – – 0 0 0
Bud Neck – – – – – – – – – – – – 0 0 0
Bud Site – – – – – – – – – – – – 0 0 0
Cell Periphery – – – – – – – – – – – – 0 0 0
Cyto – – – – – – – – – – – – – – –
Endoplasmic Reticulum – – – – – – – – – – – – 0 0 0
Endosome – – – – – – – – – – – – 0 0 0
Golgi – – – – – – – – – – – – 0 0 0
Mitochondria – – – – – – – – – – – – 0 0 0
Nuclear Periphery – – – – – – – – – – – – 0 0 0
Nuc – – – – – – – – – – – – – – –
Nucleolus – – – – – – – – – – – – 0 0 0
Peroxisomes – – – – – – – – – – – – 0 0 0
SpindlePole – – – – – – – – – – – – 0 0 0
Vac – – – – – – – – – – – – – – –
Cortical Patches – – – – – – – – – – – – 0 0 0
Cytoplasm – – – – – – – – – – – – 0 0 0
Nucleus – – – – – – – – – – – – 0 0 0
Vacuole – – – – – – – – – – – – 2.5 4.4 3.6

Images

Circle plot not available

Protein Concentration and Protein Localization Data

R1 R2 R3
G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase
Concentration 1.3044 1.3683 1.3855 0.711 1.0519 1.2968 0.9556 0.9507 0.8625 0.245 1.256 1.0568 0.2933 0.6028 0.2986 0.7238 0.5471 0.4255
Actin 0.0186 0.0062 0.0099 0.0358 0.0062 0.0134 0.0409 0.024 0.0361 0.0307 0.0359 0.0243 0.0198 0.0182 0.0136 0.0017 0.0063 0.0116
Bud 0.0009 0.0015 0.0022 0.0013 0.0012 0.0004 0.0013 0.0039 0.0051 0.0042 0.0016 0.0011 0.0017 0.0032 0.005 0.0011 0.003 0.0007
Bud Neck 0.0008 0.0007 0.0036 0.0159 0.0006 0.0014 0.002 0.0012 0.0038 0.0014 0.0011 0.0038 0.0009 0.0007 0.0016 0.0016 0.0009 0.0029
Bud Periphery 0.003 0.0034 0.0067 0.012 0.0069 0.0015 0.0028 0.0089 0.0106 0.0136 0.0037 0.0034 0.0024 0.0045 0.0078 0.0024 0.0081 0.0017
Bud Site 0.0045 0.0163 0.0099 0.0013 0.0007 0.0009 0.0078 0.0263 0.0236 0.0025 0.0041 0.0013 0.0066 0.0204 0.0107 0.0046 0.0025 0.0008
Cell Periphery 0.0014 0.0026 0.0014 0.0007 0.0005 0.0011 0.0017 0.0037 0.0056 0.0021 0.0003 0.0007 0.0007 0.0006 0.0007 0.0004 0.0006 0.0003
Cytoplasm 0.0087 0.0095 0.0085 0.0029 0.0075 0.0118 0.0301 0.0723 0.0905 0.1337 0.0039 0.0298 0.0085 0.0483 0.0169 0.0086 0.0176 0.0148
Cytoplasmic Foci 0.0426 0.0536 0.0489 0.0696 0.0367 0.0476 0.0718 0.0795 0.044 0.0385 0.0556 0.0608 0.0425 0.0631 0.0567 0.0773 0.081 0.0423
Eisosomes 0.0013 0.0033 0.0009 0.0003 0.0004 0.0005 0.0011 0.0009 0.0017 0.001 0.0003 0.0005 0.0004 0.0003 0.0004 0.0001 0.0004 0.0002
Endoplasmic Reticulum 0.0032 0.001 0.0006 0.0008 0.0016 0.0009 0.0176 0.0054 0.0074 0.0055 0.0028 0.0042 0.0018 0.0027 0.0018 0.0004 0.0028 0.002
Endosome 0.0783 0.0634 0.056 0.0617 0.0521 0.0503 0.1653 0.1199 0.1084 0.0599 0.2041 0.1121 0.1157 0.122 0.0932 0.0641 0.0849 0.0799
Golgi 0.0463 0.0501 0.0443 0.0238 0.0363 0.043 0.0845 0.0619 0.0658 0.0582 0.1659 0.0856 0.0693 0.0732 0.0553 0.0418 0.0447 0.0426
Lipid Particles 0.0258 0.0199 0.0227 0.0132 0.0348 0.0202 0.0519 0.0195 0.0117 0.0023 0.0337 0.0156 0.0388 0.0225 0.0221 0.0037 0.0075 0.027
Mitochondria 0.6282 0.659 0.6592 0.6791 0.7188 0.6827 0.3766 0.4564 0.4432 0.5673 0.4337 0.5829 0.5915 0.512 0.6201 0.7616 0.6428 0.692
None 0.058 0.039 0.0421 0.0085 0.0123 0.035 0.0535 0.0541 0.0588 0.0426 0.0009 0.0161 0.0263 0.0141 0.0257 0.003 0.0108 0.0167
Nuclear Periphery 0.0062 0.0003 0.001 0.0004 0.0009 0.0007 0.0298 0.0013 0.0031 0.0007 0.0047 0.0045 0.0052 0.001 0.0015 0.0002 0.0162 0.0021
Nucleolus 0.002 0.0006 0.0012 0.0059 0.0025 0.0004 0.0008 0.0006 0.0017 0.0004 0.0004 0.0004 0.0007 0.0004 0.0008 0.0004 0.0019 0.0004
Nucleus 0.0027 0.0006 0.0049 0.0018 0.0008 0.0005 0.0111 0.001 0.0021 0.0012 0.0006 0.001 0.0007 0.0012 0.0011 0.0003 0.0066 0.0006
Peroxisomes 0.0537 0.058 0.0657 0.0399 0.0699 0.0815 0.0226 0.0367 0.0397 0.0168 0.0268 0.0299 0.0519 0.0787 0.0512 0.0179 0.0525 0.0503
Punctate Nuclear 0.0025 0.0004 0.0018 0.0017 0.0005 0.0015 0.0085 0.0008 0.0011 0.0003 0.0021 0.0013 0.0006 0.0003 0.0012 0.0003 0.0014 0.0009
Vacuole 0.0074 0.0064 0.0059 0.0184 0.0066 0.0027 0.0106 0.0133 0.0255 0.0125 0.0103 0.0109 0.0088 0.0082 0.0088 0.0044 0.004 0.0064
Vacuole Periphery 0.0038 0.0041 0.0027 0.0051 0.0023 0.002 0.0076 0.0085 0.0105 0.0048 0.0077 0.0098 0.0052 0.0043 0.004 0.0038 0.0037 0.0036

Sequencing Data

R1 R2
G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Post-START S/G2 Metaphase Anaphase Telophase
Gene Expression 6.7944 6.4787 3.6056 8.5458 4.6982 4.5271 9.4164 10.224 10.0967 10.933
Translational Efficiency 0.6402 0.7163 1.3491 0.4939 0.9764 1.0318 0.8195 0.5034 0.6021 0.6112

Hit Data

Dataset Hit
Protein Concentration ✔
Protein Localization ✘
Gene Expression ✘
Translational Efficiency ✘

Micrographs

Cell Count

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
197 217 1582 1559 1702 1656 183 987 1899 1873 1765 2546

Protein Abundance

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
Mean 694.48 763.83 835.87 822.54 675.72 717.68 958.38 838.19 677.67 723.03 848.57 828.61
Standard Deviation 83.59 97.57 95.94 112.39 87.77 86.28 101.74 115.05 87.54 88.90 103.53 113.69
Intensity Change Log 2 — 0.137318 0.267345 0.244153 — 0.086915 0.504172 0.310852 — 0.112682 0.388994 0.277431

Localization Score (DeepLoc)

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
Actin 0.000397 0.001461 0.001757 0.008278 0.000496 0.000860 0.003485 0.006641 0.000486 0.000930 0.001936 0.007644
Bud Neck 0.030893 0.043637 0.027097 0.025681 0.032859 0.055389 0.012050 0.017372 0.032655 0.054027 0.025537 0.022460
Bud Site 0.007650 0.035839 0.027660 0.069853 0.028547 0.024278 0.034325 0.074205 0.026379 0.025618 0.028351 0.071540
Cell Periphery 0.000286 0.000255 0.000198 0.000368 0.000216 0.000288 0.000510 0.000265 0.000223 0.000284 0.000230 0.000328
Cytoplasm 0.278111* 0.196495* 0.236656* 0.288024* 0.335693* 0.214102* 0.159664* 0.347823* 0.329720* 0.212062* 0.228673* 0.311206*
Cytoplasmic Foci 0.347877* 0.322867* 0.076945 0.075581 0.239562* 0.289667* 0.117182 0.092290 0.250799* 0.293513* 0.081117 0.082058
Eisosomes 0.000382 0.000274 0.000102 0.000167 0.000121 0.000258 0.000249 0.000138 0.000148 0.000260 0.000117 0.000156
Endoplasmic Reticulum 0.000496 0.000717 0.005774 0.006218 0.001344 0.000973 0.007264 0.002501 0.001256 0.000943 0.005928 0.004777
Endosome 0.033103 0.071699 0.030255 0.018193 0.029059 0.051079 0.046115 0.017588 0.029479 0.053468 0.031899 0.017958
Golgi 0.013578 0.031696 0.011334 0.010789 0.023893 0.024075 0.014843 0.007923 0.022823 0.024958 0.011698 0.009678
Lipid Particles 0.019485 0.011041 0.008250 0.004446 0.009377 0.017348 0.014022 0.003046 0.010425 0.016618 0.008848 0.003904
Mitochondria 0.027002 0.051462 0.041350 0.015146 0.109066* 0.102479* 0.019323 0.018736 0.100553* 0.096568 0.039066 0.016538
Mitotic Spindle 0.003826 0.016985 0.058825 0.043517 0.012474 0.013831 0.032649 0.045028 0.011577 0.014197 0.056111 0.044103
None 0.001446 0.001221 0.003877 0.001335 0.005600 0.001829 0.001734 0.002026 0.005169 0.001758 0.003655 0.001603
Nuclear Periphery 0.000482 0.000364 0.004134 0.001743 0.000504 0.000480 0.002324 0.001012 0.000502 0.000466 0.003946 0.001460
Nuclear Periphery Foci 0.003351 0.000302 0.001687 0.001530 0.000594 0.000278 0.006321 0.001107 0.000880 0.000281 0.002168 0.001366
Nucleolus 0.001298 0.001962 0.006939 0.000737 0.002383 0.002902 0.003987 0.001736 0.002270 0.002793 0.006633 0.001124
Nucleus 0.131469* 0.059560 0.329334* 0.206035* 0.099629 0.073965 0.315599* 0.178095* 0.102932 0.072296 0.327910* 0.195203*
Peroxisomes 0.056916 0.089998 0.034249 0.047369 0.025099 0.052858 0.102212* 0.054735 0.028400 0.057161 0.041295 0.050224
Vacuole 0.039727 0.057427 0.087917 0.169767 0.036237 0.066654 0.103353 0.125070 0.036599 0.065585 0.089517 0.152440
Vacuole Periphery 0.002226 0.004737 0.005661 0.005224 0.007246 0.006406 0.002787 0.002663 0.006726 0.006212 0.005363 0.004231

Localization Changes (T-score)

R1 R2 R1 & R2
UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14 UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14 UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14
Actin -2.42 -7.41 -7.76 -5.15 -5.59 -2.59 -4.27 -5.46 -4.91 -1.26 -3.29 -8.02 -9.34 -8.30 -6.23
Bud Neck -1.52 0.69 1.89 3.70 2.94 -6.83 8.90 7.67 13.45 -2.06 -6.95 3.15 7.09 13.43 3.66
Bud Site -4.40 -6.96 -11.30 -1.57 -5.71 1.66 0.05 -4.86 -5.82 -3.07 0.36 0.33 -7.23 -7.31 -7.15
Cell Periphery 0.71 2.65 0.58 -0.34 -3.66 -2.38 -3.24 0.15 2.34 3.24 -2.19 0.17 -1.37 1.33 -1.45
Cytoplasm 3.34 2.50 0.69 -3.86 -3.74 12.83 10.72 1.70 -9.53 -9.04 13.27 11.67 5.26 -9.38 -7.62
Cytoplasmic Foci 1.12 17.32 18.27 16.66 3.59 -6.43 12.61 25.97 31.51 3.02 -5.77 30.83 34.60 39.65 3.17
Eisosomes 1.96 6.33 5.46 3.57 -4.81 -9.81 -6.02 -1.23 8.82 5.46 -7.99 3.86 1.44 9.39 -2.95
Endoplasmic Reticulum -1.31 -12.87 -15.43 -14.24 -0.17 1.87 -5.87 -8.70 -10.34 3.68 1.76 -12.01 -14.68 -16.08 2.32
Endosome -3.86 1.11 4.04 7.03 7.75 -7.97 -1.54 8.71 15.41 4.33 -8.87 0.65 10.60 17.88 8.97
Golgi -2.91 0.99 1.94 5.31 2.46 -0.02 3.68 10.21 10.99 2.34 -0.93 6.99 10.43 12.27 3.45
Lipid Particles 1.90 2.77 3.75 3.29 6.68 -5.77 -1.78 9.38 11.66 4.88 -4.64 2.08 9.18 11.68 8.66
Mitochondria -2.03 -1.17 2.65 4.33 8.58 0.97 12.82 17.47 17.56 0.85 0.67 12.24 18.39 18.97 8.45
Mitotic Spindle -1.95 -10.26 -7.08 -2.40 3.91 -0.64 -1.78 -5.08 -4.44 -0.73 -1.24 -10.16 -8.02 -6.20 3.91
None 0.39 -3.48 -0.40 -0.92 4.00 6.52 6.82 5.32 -1.54 -1.97 6.54 2.73 6.63 -0.61 3.28
Nuclear Periphery 0.79 -9.36 -10.86 -14.87 4.38 0.14 -4.29 -7.47 -12.39 2.24 0.27 -10.00 -12.35 -17.90 5.58
Nuclear Periphery Foci 1.03 0.57 0.56 -11.04 -0.15 1.36 -2.89 -3.47 -10.79 2.45 1.59 -2.89 -1.91 -14.89 2.19
Nucleolus -1.39 -5.67 1.38 2.42 6.28 -1.12 -0.99 1.03 1.60 1.40 -1.27 -4.71 3.06 3.60 5.91
Nucleus 4.82 -14.52 -7.15 -17.28 11.32 4.94 -10.22 -13.77 -17.09 5.00 6.26 -28.69 -20.31 -26.59 13.38
Peroxisomes -2.46 2.98 3.09 5.72 0.35 -8.62 -4.03 -2.06 4.26 3.38 -8.90 -2.35 -0.81 7.45 1.48
Vacuole -2.42 -9.81 -26.75 -22.46 -20.91 -9.34 -7.61 -22.40 -17.82 -5.25 -9.66 -17.25 -39.03 -31.16 -22.01
Vacuole Periphery -2.44 -3.68 -0.77 1.71 2.49 1.22 6.85 8.95 8.35 1.28 0.84 3.32 6.94 6.55 3.71
Standard name
Human Ortholog
Description Protein of unknown function; has weak similarity to E. coli GTP-binding protein gtp1; the authentic, non-tagged protein is detected in highly purified mitochondria in high-throughput studies
Localization
Cell Percentages mitochondrion (22%), cytoplasm (19%), mixed (9%)
Cell Cycle Regulation No
Subcompartmental Group N/A

Ylf2

Ylf2


Nearest neighbours ( genes)

correlation threshold

0.6

1.0

0.80


Biological Process (Gene Ontology)
Molecular Function (Gene Ontology)
Cellular Component (Gene Ontology)

Endocytosis
Temp Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Vph1-GFP)
37℃
RT
Cell Cycle Omics

CYCLoPs (Ylf2-GFP)
Gene / Allele Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Sac6-tdTomato)
Gene Images
Gene Images
Images are not yet available
Images are not yet available