Standard name
Human Ortholog
Description Protein required for biogenesis of 40S (small) ribosomal subunit; has similarity to the beta subunit of trimeric G-proteins and the splicing factor Prp4p; essential gene

Micrographs

ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0.08 0 0.05 0.09 0.1 0.09 0 0 0 0 0 0
Cell Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cytoplasm 0 0 0.05 0.11 0.07 0 0.06 0.05 0 0.05 0 0 0.11 0.09 0.11 0.06 0.08 0 0 0 0 0 0 0
Endoplasmic Reticulum 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Endosome 0 0 0 0 0.05 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Golgi 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Mitochondria 0.16 0.1 0 0.17 0.06 0.18 0.23 0.24 0.27 0.24 0.26 0.33 0 0 0 0.16 0.06 0 0 0 0 0 0 0
Nucleus 0.15 0.2 0.07 0.11 0 0.07 0.08 0.06 0.05 0 0.07 0.08 0.11 0 0.07 0.17 0.27 0.2 0.1 0.2 0 0 0 0
Nuclear Periphery 0 0 0.05 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0.94 0.88 0.91 0.83 0.82 0.86 0.76 0.78 0.83 0.78 0.84 0.79 0.81 0.86 0.8 0.81 0.76 0.79 0.81 0.71 0.69 0.82 0.81 0.72
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0.07 0.17 0.22 0.35 0.38 0.42 0.32 0.37 0.3 0.31 0 0.08 0.09 0 0 0 0 0 0.15 0.1 0.09 0.09
Vac/Vac Membrane 0 0 0 0 0.05 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0.05
ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 0 0 1 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud 1 0 0 0 0 2 1 2 3 4 1 0 0 0 0 1 0 0 2 2 0 1 1 3
Bud Neck 3 0 0 0 2 1 3 3 1 3 3 1 2 4 1 1 1 0 0 0 0 0 2 4
Bud Site 1 0 0 0 0 1 8 6 5 8 7 11 1 1 0 1 0 2
Cell Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 1 1 0 0 0 0 0 0 0 0
Cytoplasm 1 2 2 2 6 3 6 8 4 5 3 3 25 18 27 16 18 8 0 0 1 0 0 0
Endoplasmic Reticulum 0 0 0 0 0 0 0 1 0 0 0 0 0 0 0 2 3 4 0 0 0 0 1 1
Endosome 0 1 1 0 4 2 0 0 3 0 2 3 4 3 6 6 2 1 3 0 1 0 3 5
Golgi 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 2
Mitochondria 45 10 1 3 5 13 24 38 30 22 18 40 5 5 6 40 12 6 2 1 0 0 1 1
Nucleus 40 20 3 2 3 5 8 10 5 3 5 10 24 7 17 43 58 36 27 23 2 4 5 4
Nuclear Periphery 0 1 2 0 2 0 0 0 1 2 1 1 0 0 0 2 2 0 2 0 0 0 0 2
Nucleolus 258 86 40 15 72 64 79 125 92 71 58 95 179 178 205 202 165 140 223 85 41 95 162 172
Peroxisomes 3 1 0 0 0 1 1 0 0 2 0 1 2 6 9 1 0 0 1 0 0 0 0 0
SpindlePole 2 1 3 3 19 26 39 67 35 34 21 37 9 17 24 2 5 2 6 3 8 11 18 21
Vac/Vac Membrane 0 0 1 0 4 0 1 0 0 0 0 2 1 0 1 3 7 4 3 2 0 0 1 11
Unique Cell Count 275 98 44 18 88 74 104 160 111 91 69 121 220 207 255 248 218 178 277 120 61 116 200 241
Labelled Cell Count 354 122 53 25 117 118 170 260 180 154 119 204 252 239 297 321 273 203 277 120 61 116 200 241


Nucleolus

(Huh et al. Nature 2003 425:686)
Screen WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 AF100 AF140 AF180
Mean Cell GFP Intensity (1e-4) 16.5 17.9 10.5 9.1 10.8 6.7 5.2 5.4 5.4 5.3 5.0 4.6 15.4 14.7 14.7 17.0 18.5 21.3 14.0 16.3 16.4
Std Deviation (1e-4) 4.5 5.4 2.6 2.5 6.7 1.7 1.6 1.8 1.4 2.2 1.3 1.5 6.5 5.7 6.4 7.3 9.7 9.5 5.2 5.3 4.8
Intensity Change (Log2) -0.21 0.04 -0.66 -1.02 -0.96 -0.96 -0.98 -1.07 -1.19 0.55 0.49 0.48 0.69 0.82 1.02 0.41 0.64 0.64


Localization RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3
Actin
Bud 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cell Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cyto
Endoplasmic Reticulum 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Endosome 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Golgi 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Mitochondria 0 0 2.5 3.1 3.2 3.5 3.2 3.3 0 0 0 2.4 0 0
Nuclear Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nuc
Nucleolus -0.9 -1.4 -0.7 -2.1 -1.9 -1.3 -1.8 -1.0 -1.5 -0.9 -1.7 -1.5 -2.2 -1.9
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 2.1 3.5 3.8 4.3 3.2 3.7 3.0 0 0 0 0 0 0
Vac
Cortical Patches 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cytoplasm 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleus 0 0 0 0 0 0 0 0 0 0 0 1.8 2.8 2.1
Vacuole 0 0 0 0 0 0 0 0 0 0 0 0 0 0

Images

Circle plot not available

Protein Concentration and Protein Localization Data

R1 R2 R3
G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase
Concentration 11.1068 13.2152 12.7996 11.5586 11.4612 12.0837 12.0714 15.8342 13.2054 11.8098 12.6226 13.4014 13.605 16.3659 14.8632 14.3921 13.598 14.9704
Actin 0.0262 0 0 0 0.0252 0.0012 0.0014 0 0.0088 0.0001 0.0001 0.0003 0.0162 0.0025 0.0057 0.0022 0.0262 0.001
Bud 0.0002 0 0 0.0001 0.0002 0 0 0 0.0001 0 0.0001 0 0.0003 0.0002 0.0002 0.0001 0.0008 0
Bud Neck 0.0006 0.0001 0 0.0006 0.0004 0.0001 0.0001 0.0001 0.0003 0.0002 0.0003 0.0001 0.001 0.0004 0.0008 0.0001 0.0003 0.0001
Bud Periphery 0.0003 0 0 0.0001 0.0002 0.0001 0 0 0.0002 0 0.0001 0 0.0003 0.0002 0.0001 0.0001 0.0022 0.0001
Bud Site 0.0011 0.0001 0 0.0002 0.0009 0.0001 0.0003 0 0.0016 0.0001 0.0003 0.0001 0.0073 0.0043 0.0029 0.0004 0.0004 0.0001
Cell Periphery 0.0003 0 0.0001 0.0002 0.0002 0.0001 0.0001 0 0.0002 0.0002 0.0002 0 0.0004 0.0001 0.0002 0.0001 0.0002 0
Cytoplasm 0.0039 0 0 0 0.0002 0.0004 0.0044 0 0.0001 0.0004 0 0.0001 0.0045 0.0128 0.0007 0.0011 0.0004 0.0022
Cytoplasmic Foci 0.0294 0.0001 0.0001 0.003 0.0054 0.0005 0.0249 0.0001 0.002 0.006 0.0018 0.0035 0.0136 0.0038 0.0131 0.0098 0.0012 0.0022
Eisosomes 0.0006 0 0 0 0.0001 0 0 0 0.0004 0.0001 0.0001 0 0.0003 0 0.0001 0 0.0002 0
Endoplasmic Reticulum 0.0013 0 0 0 0.0003 0.0003 0.0009 0 0.0002 0.0002 0 0 0.0039 0.0002 0.004 0.001 0.0023 0.0004
Endosome 0.0091 0 0 0.0001 0.0033 0.0032 0.0094 0 0.0053 0.0004 0.0003 0.0036 0.0232 0.0022 0.0083 0.016 0.007 0.001
Golgi 0.003 0 0 0 0.0042 0.0034 0.0045 0 0.0015 0.0002 0.0006 0.003 0.0075 0.0022 0.0068 0.0038 0.0026 0.0005
Lipid Particles 0.0178 0.0001 0.0002 0.002 0.0054 0.0019 0.0433 0.0002 0.0138 0.0088 0.0168 0.0046 0.0066 0.0037 0.0105 0.0219 0.0068 0.0035
Mitochondria 0.0066 0 0.0001 0.0012 0.0011 0.0019 0.001 0 0.0051 0.0037 0.0216 0.0006 0.0044 0.0036 0.0025 0.0027 0.0106 0.0023
None 0.009 0 0 0.0001 0.0002 0.0001 0.0047 0 0.0004 0.0005 0.0001 0.0001 0.0047 0.0002 0.0005 0.0011 0.0007 0.0043
Nuclear Periphery 0.0062 0 0 0 0.0006 0.0029 0.0038 0 0.0041 0.0124 0.0009 0.0002 0.0206 0.0004 0.004 0.0015 0.0174 0.0009
Nucleolus 0.7903 0.9136 0.9105 0.9076 0.8488 0.8885 0.7881 0.918 0.8862 0.8751 0.8981 0.9056 0.8005 0.8557 0.8502 0.8081 0.8455 0.9042
Nucleus 0.0581 0.0857 0.0887 0.0815 0.0971 0.0872 0.0722 0.0809 0.062 0.0613 0.0516 0.0656 0.0649 0.1038 0.0781 0.1141 0.0594 0.0709
Peroxisomes 0.0049 0 0 0.0002 0.0024 0.0001 0.0214 0 0.0031 0.0003 0.0026 0.0017 0.0118 0.0004 0.0042 0.0087 0.0006 0.0006
Punctate Nuclear 0.0273 0.0002 0.0002 0.0009 0.0033 0.0073 0.0188 0.0004 0.0014 0.0273 0.0031 0.0104 0.0034 0.0024 0.0059 0.0021 0.0126 0.0054
Vacuole 0.0018 0.0001 0.0001 0.0016 0.0004 0.0004 0.0003 0.0001 0.0014 0.0004 0.0004 0.0002 0.0021 0.0006 0.0008 0.0032 0.0008 0.0001
Vacuole Periphery 0.002 0 0 0.0004 0.0003 0.0003 0.0003 0 0.002 0.0018 0.0007 0.0001 0.0024 0.0003 0.0008 0.0019 0.0017 0.0001

Sequencing Data

R1 R2
G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Post-START S/G2 Metaphase Anaphase Telophase
Gene Expression 47.2888 42.2733 37.3436 39.2816 32.1158 27.7574 39.6894 25.185 29.4001 39.667
Translational Efficiency 1.2835 1.3867 1.3755 1.0871 1.3121 1.4777 1.0097 0.9895 0.9744 1.0797

Hit Data

Dataset Hit
Protein Concentration
Protein Localization
Gene Expression
Translational Efficiency

Micrographs

Cell Count

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
2381 1077 329 1381 2961 1719 193 118 5342 2796 522 1499

Protein Abundance

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
Mean 1309.28 1386.74 1640.84 1968.07 1409.59 1460.79 1783.02 1993.80 1364.88 1432.27 1693.41 1970.10
Standard Deviation 272.18 388.48 320.80 501.13 269.09 329.05 285.81 411.07 275.03 354.96 315.87 494.69
Intensity Change Log 2 0.082924 0.325661 0.588008 0.051473 0.339047 0.500245 0.066704 0.332617 0.543174

Localization Score (DeepLoc)

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
Actin 0.000270 0.000321 0.000474 0.000557 0.000203 0.000284 0.000247 0.000636 0.000233 0.000299 0.000390 0.000564
Bud Neck 0.001482 0.001411 0.003672 0.006274 0.000901 0.001562 0.002719 0.010111 0.001160 0.001504 0.003319 0.006576
Bud Site 0.000447 0.000828 0.000868 0.001765 0.000135 0.000349 0.000628 0.001601 0.000274 0.000533 0.000779 0.001752
Cell Periphery 0.000028 0.000072 0.000033 0.000086 0.000009 0.000032 0.000013 0.000055 0.000018 0.000047 0.000026 0.000084
Cytoplasm 0.000426 0.000848 0.000102 0.000591 0.000240 0.000081 0.000082 0.000221 0.000323 0.000377 0.000095 0.000562
Cytoplasmic Foci 0.003093 0.002863 0.002427 0.002417 0.000582 0.002491 0.002547 0.005001 0.001701 0.002634 0.002471 0.002620
Eisosomes 0.000542 0.000239 0.000132 0.000200 0.000462 0.000317 0.000139 0.000145 0.000498 0.000287 0.000134 0.000196
Endoplasmic Reticulum 0.000235 0.000393 0.000533 0.000208 0.000157 0.000192 0.000122 0.000313 0.000192 0.000269 0.000381 0.000216
Endosome 0.000109 0.000237 0.000126 0.000398 0.000028 0.000084 0.000024 0.004725 0.000064 0.000143 0.000088 0.000739
Golgi 0.000228 0.000357 0.000371 0.000803 0.000072 0.000141 0.000300 0.003541 0.000141 0.000224 0.000345 0.001019
Lipid Particles 0.000070 0.000198 0.000074 0.000082 0.000009 0.000132 0.000015 0.005827 0.000036 0.000158 0.000052 0.000534
Mitochondria 0.000808 0.001331 0.001424 0.003143 0.000524 0.001575 0.000880 0.001837 0.000651 0.001481 0.001223 0.003041
Mitotic Spindle 0.000622 0.001733 0.012649 0.007299 0.001375 0.002558 0.009122 0.006431 0.001039 0.002240 0.011345 0.007231
None 0.000614 0.001418 0.000515 0.000595 0.000182 0.000398 0.000358 0.000516 0.000375 0.000791 0.000457 0.000589
Nuclear Periphery 0.000055 0.000123 0.000172 0.000509 0.000045 0.000351 0.000073 0.000364 0.000050 0.000263 0.000136 0.000498
Nuclear Periphery Foci 0.000343 0.000551 0.000202 0.000337 0.000162 0.000588 0.000070 0.000529 0.000242 0.000574 0.000153 0.000352
Nucleolus 0.651011* 0.665397* 0.652320* 0.679040* 0.667385* 0.675076* 0.719029* 0.654650* 0.660087* 0.671347* 0.676984* 0.677120*
Nucleus 0.285953* 0.267273* 0.240668* 0.273971* 0.287632* 0.271956* 0.182666* 0.276959* 0.286883* 0.270152* 0.219223* 0.274206*
Peroxisomes 0.052428 0.052678 0.082111 0.015868 0.039250 0.040199 0.080060 0.022217 0.045124 0.045006 0.081353 0.016368
Vacuole 0.000664 0.000980 0.000563 0.002320 0.000303 0.000877 0.000440 0.002542 0.000464 0.000917 0.000518 0.002338
Vacuole Periphery 0.000573 0.000750 0.000566 0.003536 0.000343 0.000757 0.000466 0.001778 0.000445 0.000754 0.000529 0.003398

Localization Changes (T-score)

R1 R2 R1 & R2
UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14 UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14 UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14
Actin -1.82 -5.88 -11.79 -7.94 -2.09 -4.84 -2.42 -4.07 -3.54 -3.39 -4.34 -6.82 -14.71 -10.96 -5.06
Bud Neck 0.42 -6.90 -9.74 -9.66 -5.01 -4.13 -4.67 -5.12 -4.91 -4.33 -2.90 -8.69 -11.46 -10.72 -6.59
Bud Site -0.97 -3.52 -3.49 -2.44 -2.59 -3.16 -7.27 -5.13 -4.68 -3.97 -1.69 -7.11 -4.28 -3.66 -3.08
Cell Periphery -1.37 -0.59 -3.82 -0.67 -3.10 -2.86 -2.02 -3.93 -2.28 -3.54 -2.27 -1.48 -4.77 -2.25 -3.90
Cytoplasm -0.97 1.62 -0.77 0.15 -2.10 0.81 0.49 -0.05 -1.93 -0.96 -0.08 1.45 -1.13 -1.15 -2.09
Cytoplasmic Foci 0.31 0.28 0.67 0.35 0.27 -3.11 -5.14 -1.74 -0.77 -0.75 -1.92 -1.96 -1.75 -0.12 0.04
Eisosomes 10.14 15.23 11.75 1.98 -4.36 6.54 17.89 11.55 6.44 -0.18 11.53 23.37 15.78 5.57 -4.38
Endoplasmic Reticulum -1.71 -3.73 0.03 1.64 3.51 -2.25 -0.36 -2.19 -1.95 -2.00 -2.11 -3.91 -1.80 0.31 2.32
Endosome -1.18 -0.14 -1.36 -0.75 -1.28 -1.26 0.21 -1.28 -1.26 -1.28 -1.55 -0.40 -2.00 -1.74 -1.92
Golgi -0.74 -1.79 -1.08 -0.83 -0.78 -2.82 -8.29 -1.33 -1.30 -1.22 -1.15 -5.03 -1.64 -1.48 -1.19
Lipid Particles -0.93 -0.03 -0.28 0.85 -0.29 -1.85 -1.80 -1.08 -1.05 -1.08 -1.83 -0.77 -1.20 -0.82 -1.15
Mitochondria -2.59 -4.68 -3.73 -2.80 -2.66 -1.81 -3.08 -5.93 -1.13 -3.74 -2.27 -6.12 -4.19 -2.40 -3.05
Mitotic Spindle -2.03 -3.24 -4.60 -3.72 1.24 -1.30 -1.83 -2.77 -2.19 0.24 -2.03 -3.66 -4.64 -3.64 1.15
None -2.01 0.79 -0.02 2.09 -1.42 -5.87 -3.31 -5.59 -2.81 -2.32 -2.63 -1.27 -3.99 1.10 -2.54
Nuclear Periphery -1.98 -3.74 -7.39 -5.66 -4.95 -1.41 -2.30 -4.67 -0.56 -4.03 -1.59 -4.43 -8.02 -1.80 -6.02
Nuclear Periphery Foci -1.03 1.44 0.26 1.48 -2.40 -1.88 1.43 -1.31 0.30 -1.78 -2.06 1.61 -1.14 1.56 -3.89
Nucleolus -1.67 0.11 -2.48 -0.77 -1.56 -1.09 -2.87 1.86 2.10 3.01 -2.08 -1.10 -1.05 0.32 0.25
Nucleus 2.16 3.14 1.10 -0.89 -2.35 2.27 6.55 -0.68 -1.26 -3.86 3.10 6.07 1.07 -1.05 -4.62
Peroxisomes -0.11 -5.64 22.41 16.95 12.35 -0.67 -6.77 6.76 6.87 9.04 0.02 -9.01 26.72 22.33 15.96
Vacuole -1.17 1.05 -4.31 -3.13 -4.57 -2.58 -1.33 -2.98 -2.45 -2.77 -2.67 -0.98 -5.29 -3.98 -5.09
Vacuole Periphery -2.03 -0.01 -7.32 -6.77 -7.28 -4.76 -5.90 -6.01 -4.05 -5.41 -4.87 -2.50 -7.94 -7.01 -7.65
Standard name
Human Ortholog
Description Protein required for biogenesis of 40S (small) ribosomal subunit; has similarity to the beta subunit of trimeric G-proteins and the splicing factor Prp4p; essential gene
Localization
Cell Percentages nucleolus (97%)
Cell Cycle Regulation No
Subcompartmental Group nucleolus-2

Sof1

Sof1


Nearest neighbours ( genes)

correlation threshold

0.6

1.0

0.80


Biological Process (Gene Ontology)

Molecular Function (Gene Ontology)

Cellular Component (Gene Ontology)


Endocytosis
Temp Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Vph1-GFP)
37℃
RT
Cell Cycle Omics

CYCLoPs (Sof1-GFP)
Gene / Allele Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Sac6-tdTomato)
Gene Images
Gene Images
Images are not yet available
Images are not yet available