Standard name
Human Ortholog
Description Protein required for cytochrome c oxidase assembly; located in the cytosol and mitochondrial intermembrane space; putative copper metallochaperone that delivers copper to cytochrome c oxidase; contains twin cysteine-x9-cysteine motifs
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Micrographs

ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 – – – – – –
Cell Periphery 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cytoplasm 0.98 0.99 0.98 0.95 0.89 0.82 – 0.6 0.57 0.6 0.55 0.6 0.98 0.98 0.98 0.96 0.93 0.95 0.93 0.93 0.95 0.9 0.85 0.92
Endoplasmic Reticulum 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Endosome 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Golgi 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Mitochondria 0 0 0 0.07 0.17 0.27 – 0.52 0.58 0.58 0.58 0.58 0 0 0 0 0 0 0 0 0 0 0 0
Nucleus 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nuclear Periphery 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Vac/Vac Membrane 0 0 0 0 0 0.05 – 0.08 0.06 0.08 0.05 0 0 0 0 0 0 0 0 0 0 0 0 0
ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 1 2 1
Bud 0 2 2 0 3 0 – 9 5 5 4 4 0 0 0 0 0 0 1 1 0 2 4 3
Bud Neck 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 1 0 0 0
Bud Site 0 0 0 0 0 0 – 0 0 1 0 1 0 0 0 0 0 0 – – – – – –
Cell Periphery 0 1 0 1 2 0 – 1 3 3 6 2 2 0 1 4 1 5 0 0 0 0 0 0
Cytoplasm 326 398 344 210 142 305 – 267 220 213 176 133 499 394 508 129 98 114 322 370 344 226 272 334
Endoplasmic Reticulum 1 2 1 2 2 2 – 1 2 1 3 3 5 2 6 4 2 3 0 3 1 0 3 3
Endosome 0 0 0 0 0 0 – 1 0 3 1 0 0 0 0 0 1 1 4 5 2 0 3 3
Golgi 0 0 0 0 0 0 – 0 0 0 0 0 0 1 0 0 3 1 3 1 0 1 5 1
Mitochondria 4 1 5 16 27 102 – 232 225 205 186 129 0 0 1 0 1 5 3 1 2 0 1 1
Nucleus 0 0 0 0 5 4 – 5 5 5 11 5 13 5 5 4 1 0 5 4 2 5 7 1
Nuclear Periphery 0 0 0 1 0 0 – 1 4 1 0 1 0 0 0 0 0 0 1 1 0 2 0 1
Nucleolus 0 0 0 1 0 0 – 0 1 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 1 0
SpindlePole 0 0 0 0 0 0 – 0 0 1 0 0 0 0 0 0 0 0 0 0 1 0 1 1
Vac/Vac Membrane 3 3 4 4 4 18 – 37 23 29 16 7 7 4 6 4 3 4 1 3 1 7 11 5
Unique Cell Count 333 402 351 221 159 373 448 385 353 320 221 511 400 519 135 105 120 347 400 364 252 322 363
Labelled Cell Count 334 407 356 235 185 431 554 488 467 403 285 526 406 527 145 110 133 347 400 364 252 322 363


Cytoplasm, Nucleus

(Huh et al. Nature 2003 425:686)
Screen WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 AF100 AF140 AF180
Mean Cell GFP Intensity (1e-4) 9.5 12.6 6.8 7.5 4.7 4.8 – 4.3 3.9 3.9 4.0 4.1 12.2 9.9 7.8 11.2 11.0 10.1 12.6 11.1 10.1
Std Deviation (1e-4) 1.6 2.0 1.3 1.8 1.6 1.1 – 1.0 1.0 0.8 1.1 0.7 2.3 1.6 1.5 2.6 2.4 2.4 2.3 2.2 1.5
Intensity Change (Log2) – – – 0.14 -0.54 -0.51 – -0.67 -0.82 -0.79 -0.76 -0.74 0.84 0.54 0.2 0.72 0.68 0.57 0.88 0.7 0.56


Localization RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3
Cortical Patches 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0
Bud 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0
Cell Periphery 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0
Cytoplasm -2.0 -4.3 -7.2 – -12.7 -13.1 -12.3 -13.3 -11.8 -0.3 0.5 -0.1 -1.5 -2.4 -1.7
Endoplasmic Reticulum 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0
Endosome 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0
Golgi 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0
Mitochondria 3.6 0 0 – 0 0 0 0 0 0 0 0 0 0 0
Nucleus 0 0 0 – 0 0 0 3.5 0 3.0 0 0 0 0 0
Nuclear Periphery 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0
Vacuole 0 0 2.9 – 4.5 3.5 4.4 2.9 0 0 0 0 0 0 0

Images

Circle plot not available

Protein Concentration and Protein Localization Data

R1 R2 R3
G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase
Concentration 6.8873 8.9029 8.6504 7.5174 7.5111 8.2233 5.4718 7.2407 6.548 5.6343 6.2623 6.248 8.422 9.8896 9.5578 8.707 9.2043 9.1506
Actin 0.0065 0.0008 0.0071 0.0012 0.0312 0.0006 0.0169 0.0024 0.0218 0.0036 0.0402 0.0102 0.0008 0.0004 0.0003 0.0005 0.0008 0.0006
Bud 0.0078 0.0015 0.0063 0.0071 0.0001 0.0042 0.0011 0.0002 0.0025 0.0014 0.0037 0.0003 0.0002 0.0001 0.0001 0.0002 0.0001 0.0001
Bud Neck 0.0049 0.0006 0.0015 0.0018 0.0014 0.0053 0.0057 0.0002 0.0029 0.0038 0.0026 0.0012 0.0009 0.0006 0.0016 0.001 0.0008 0.0026
Bud Periphery 0.001 0.0004 0.0017 0.0042 0.0002 0.0011 0.0015 0 0.003 0.002 0.0046 0.0002 0.0001 0 0 0 0 0
Bud Site 0.002 0.0047 0.0025 0.0012 0.0003 0.0034 0.0035 0.0005 0.0027 0.0117 0.0161 0.0002 0.0005 0.0007 0.0029 0.0002 0.0001 0.0001
Cell Periphery 0.0001 0 0.0001 0.0003 0.0001 0 0.0003 0 0.0002 0.0003 0.0004 0 0 0 0 0 0 0
Cytoplasm 0.8609 0.9366 0.8894 0.8509 0.83 0.8582 0.8148 0.9624 0.8471 0.7822 0.7216 0.8654 0.8766 0.9263 0.8997 0.851 0.845 0.8844
Cytoplasmic Foci 0.0072 0.0017 0.0028 0.011 0.002 0.0039 0.0118 0.0029 0.0108 0.0216 0.022 0.0061 0.007 0.0025 0.0022 0.0029 0.0033 0.003
Eisosomes 0.0001 0 0 0 0.0013 0 0.0001 0 0.0001 0.0001 0.0002 0 0 0 0 0 0.0001 0
Endoplasmic Reticulum 0.0061 0.0016 0.0018 0.0038 0.0029 0.0023 0.0073 0.0013 0.0032 0.0047 0.007 0.0035 0.0041 0.0019 0.0016 0.0028 0.0026 0.0033
Endosome 0.009 0.0007 0.0011 0.0011 0.0021 0.0016 0.0288 0.001 0.0166 0.0457 0.0536 0.0113 0.0049 0.0018 0.0011 0.0018 0.0024 0.0017
Golgi 0.0008 0.0001 0.0002 0.0001 0.0019 0.0001 0.0066 0.0002 0.0068 0.008 0.0197 0.0044 0.0007 0.0001 0.0001 0.0001 0.0002 0.0001
Lipid Particles 0.0004 0 0 0.0001 0.0012 0 0.0064 0 0.0023 0.0079 0.0081 0.0009 0.0005 0 0 0 0.0019 0
Mitochondria 0.002 0.0001 0.0008 0.0003 0.0005 0.0003 0.0084 0.0002 0.0029 0.0034 0.0099 0.0036 0.0005 0.0002 0.0002 0.0003 0.0359 0.0002
None 0.0108 0.0029 0.0039 0.0276 0.006 0.0035 0.0168 0.0041 0.0025 0.0098 0.007 0.0025 0.0142 0.0087 0.0033 0.0064 0.0069 0.0036
Nuclear Periphery 0.0113 0.0026 0.0063 0.0061 0.0089 0.0048 0.0109 0.0019 0.0104 0.0075 0.018 0.0067 0.0053 0.0032 0.0031 0.0055 0.0055 0.0051
Nucleolus 0.0005 0.0001 0.0003 0.0005 0.0002 0.0001 0.0004 0 0.0002 0.0027 0.0011 0.0002 0.0004 0.0002 0.0001 0.0002 0.0001 0.0001
Nucleus 0.0602 0.0433 0.0695 0.0692 0.1053 0.106 0.0419 0.0209 0.0539 0.0524 0.0385 0.0734 0.0754 0.0482 0.081 0.1226 0.0828 0.091
Peroxisomes 0.0005 0.0001 0.0002 0.0002 0.0008 0.0001 0.0057 0.0004 0.0023 0.0019 0.0049 0.0004 0.0009 0.0001 0.0001 0.0001 0.0047 0.0002
Punctate Nuclear 0.0044 0.0016 0.0033 0.0095 0.0026 0.0036 0.005 0.0008 0.0035 0.0205 0.0121 0.0081 0.0056 0.0041 0.0022 0.0034 0.0059 0.0034
Vacuole 0.0028 0.0006 0.0008 0.0034 0.0007 0.0007 0.0038 0.0004 0.0035 0.0075 0.0051 0.001 0.0013 0.0008 0.0004 0.001 0.0008 0.0006
Vacuole Periphery 0.0008 0.0001 0.0003 0.0003 0.0003 0.0001 0.0023 0.0001 0.0007 0.0011 0.004 0.0004 0.0002 0.0001 0.0001 0.0002 0.0002 0.0001

Sequencing Data

R1 R2
G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Post-START S/G2 Metaphase Anaphase Telophase
Gene Expression 33.4365 36.5325 32.0375 27.8702 33.7181 37.1311 26.8639 32.8631 39.6323 26.8623
Translational Efficiency 1.1325 1.1303 1.3004 1.6162 1.2991 1.0625 1.2362 1.5451 1.1915 1.3475

Hit Data

Dataset Hit
Protein Concentration ✘
Protein Localization ✔
Gene Expression ✘
Translational Efficiency ✘

Micrographs

Cell Count

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
330 1459 711 1189 1202 1921 19 757 1532 3380 730 1946

Protein Abundance

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
Mean 713.07 1030.44 1085.52 1093.51 965.50 953.17 485.27 1093.52 911.13 986.52 1069.90 1093.51
Standard Deviation 101.20 178.07 190.81 180.39 151.30 158.37 98.48 163.21 175.89 171.48 211.77 173.91
Intensity Change Log 2 — 0.531145 0.606271 0.616851 — -0.018543 -0.992489 0.179631 — 0.240896 -0.095742 0.381740

Localization Score (DeepLoc)

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
Actin 0.000167 0.000531 0.000296 0.004213 0.000226 0.000421 0.003218 0.002215 0.000213 0.000469 0.000372 0.003436
Bud Neck 0.006518 0.000983 0.002076 0.011682 0.000871 0.001316 0.015065 0.004662 0.002088 0.001172 0.002414 0.008951
Bud Site 0.013990 0.004088 0.007423 0.036884 0.003027 0.004402 0.050537 0.039094 0.005389 0.004266 0.008545 0.037744
Cell Periphery 0.000269 0.000061 0.000336 0.000152 0.000131 0.000095 0.017626 0.000206 0.000161 0.000081 0.000786 0.000173
Cytoplasm 0.667222* 0.795933* 0.443847* 0.510819* 0.770218* 0.744434* 0.023877 0.484093* 0.748032* 0.766664* 0.432916* 0.500422*
Cytoplasmic Foci 0.172385 0.028165 0.018635 0.009882 0.045642 0.033356 0.006076 0.027689 0.072943 0.031115 0.018308 0.016809
Eisosomes 0.000064 0.000011 0.000076 0.000021 0.000017 0.000017 0.000738 0.000032 0.000027 0.000014 0.000093 0.000025
Endoplasmic Reticulum 0.002123 0.000693 0.002725 0.002280 0.002204 0.001339 0.018194 0.002365 0.002187 0.001060 0.003127 0.002313
Endosome 0.005791 0.001931 0.003301 0.008620 0.002264 0.002668 0.000593 0.020686 0.003024 0.002350 0.003230 0.013314
Golgi 0.002274 0.000688 0.000131 0.003515 0.000338 0.001545 0.000297 0.016948 0.000755 0.001175 0.000136 0.008740
Lipid Particles 0.003432 0.000455 0.001855 0.001027 0.000948 0.001089 0.012687 0.001526 0.001483 0.000816 0.002137 0.001221
Mitochondria 0.002427 0.001680 0.000323 0.005188 0.000160 0.000845 0.002371 0.032494 0.000649 0.001206 0.000376 0.015810
Mitotic Spindle 0.000341 0.001590 0.001510 0.088330 0.000528 0.001418 0.000170 0.082451 0.000488 0.001492 0.001475 0.086043
None 0.009056 0.007696 0.015379 0.009883 0.004057 0.010743 0.225213* 0.009682 0.005134 0.009428 0.020841 0.009805
Nuclear Periphery 0.000248 0.000191 0.000886 0.001581 0.000287 0.000267 0.000354 0.000970 0.000279 0.000234 0.000872 0.001344
Nuclear Periphery Foci 0.000741 0.003049 0.003824 0.004415 0.006282 0.003309 0.011217 0.005184 0.005088 0.003197 0.004017 0.004715
Nucleolus 0.000872 0.000349 0.001097 0.000373 0.000196 0.000507 0.023792 0.000208 0.000342 0.000439 0.001688 0.000309
Nucleus 0.080859 0.120082 0.401125* 0.196656* 0.126477 0.161459* 0.575267* 0.114865 0.116651 0.143598* 0.405658* 0.164839*
Peroxisomes 0.001474 0.000391 0.000377 0.000621 0.000212 0.000900 0.000789 0.012756 0.000484 0.000680 0.000388 0.005342
Vacuole 0.028271 0.031187 0.094562 0.097202 0.035709 0.029508 0.011758 0.129374 0.034107 0.030233 0.092406 0.109717
Vacuole Periphery 0.001476 0.000247 0.000215 0.006656 0.000205 0.000362 0.000162 0.012500 0.000478 0.000312 0.000214 0.008929

Localization Changes (T-score)

R1 R2 R1 & R2
UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14 UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14 UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14
Actin -1.36 -7.78 -1.98 -1.18 -1.37 -1.35 -1.58 -3.24 -1.65 1.33 -1.79 -4.55 -2.55 -1.64 -1.44
Bud Neck 4.45 2.92 0.38 -7.60 -4.60 -1.41 -2.14 -9.24 -5.97 1.80 2.86 -2.72 -5.81 -8.77 -3.51
Bud Site 3.59 2.28 0.12 -5.40 -2.92 -1.52 -1.91 -5.88 -4.82 1.51 1.47 -1.36 -5.87 -7.05 -2.64
Cell Periphery 7.34 -0.67 4.72 -8.86 8.94 3.24 -2.04 -0.26 -2.88 2.04 7.78 -2.39 2.45 -6.92 2.51
Cytoplasm -8.16 17.13 17.65 42.49 -1.22 3.65 52.12 32.94 31.26 -22.84 -1.95 33.04 43.70 51.82 -1.86
Cytoplasmic Foci 15.60 17.37 18.26 12.69 6.06 4.35 11.86 13.83 11.45 -2.32 13.07 18.90 21.52 17.91 4.44
Eisosomes 8.85 -0.97 6.26 -7.88 9.77 0.05 -1.73 -10.35 -9.80 1.69 6.63 -4.93 -2.10 -12.00 4.66
Endoplasmic Reticulum 2.91 -3.10 0.58 -7.25 6.78 4.63 -2.99 3.89 -0.72 3.14 6.49 -5.77 2.80 -5.17 8.00
Endosome 6.50 3.93 5.63 -0.51 2.95 -1.23 6.01 -1.71 -1.06 -3.98 2.44 -1.24 0.89 -0.76 1.68
Golgi 3.18 4.43 3.10 -0.01 -1.89 -2.53 0.19 -2.08 -0.60 -2.08 -1.26 4.95 -1.18 -0.15 -2.85
Lipid Particles 7.71 3.64 4.66 -14.63 2.28 -0.76 -0.95 -4.41 -2.43 0.89 4.23 -2.11 -1.28 -6.40 1.69
Mitochondria 1.00 5.60 1.70 -0.04 -2.76 -2.85 -2.65 -2.51 -2.07 -0.98 -1.06 2.42 -2.79 -1.83 -3.15
Mitotic Spindle -1.96 -3.06 -9.21 -8.76 -8.41 -1.56 1.56 -7.30 -7.01 -7.40 -2.28 -2.80 -11.70 -11.22 -10.57
None 0.95 -0.73 0.49 -1.21 1.78 -5.76 -2.50 -4.27 2.41 2.46 -4.58 -3.81 -3.98 1.48 2.90
Nuclear Periphery 0.49 -14.78 -19.43 -22.48 -6.95 0.47 -0.56 -15.76 -16.48 -10.82 0.92 -16.19 -24.61 -27.00 -6.43
Nuclear Periphery Foci -6.99 -7.56 -6.52 2.21 4.44 4.93 -0.77 5.21 0.45 1.29 3.76 -0.18 5.41 2.26 4.20
Nucleolus 1.69 0.17 1.75 -0.16 4.08 -3.73 -1.59 -2.49 2.87 1.58 -1.11 -2.63 0.24 1.66 2.71
Nucleus -6.91 -28.62 -21.21 -18.95 12.72 -6.92 -4.81 -6.71 -1.92 4.26 -7.30 -28.07 -19.44 -15.16 16.75
Peroxisomes 6.15 6.47 7.78 2.17 1.35 -2.50 -1.33 -1.73 -0.83 -1.07 -1.37 1.84 -0.98 -0.24 -1.39
Vacuole -3.02 -14.37 -25.91 -25.75 -9.76 2.15 3.99 -26.94 -27.77 -25.52 0.02 -14.24 -36.96 -37.75 -14.55
Vacuole Periphery 6.74 7.01 0.76 -1.73 -1.79 -2.73 0.63 -2.22 -1.97 -2.28 3.14 5.11 -2.06 -2.52 -2.78
Standard name
Human Ortholog
Description Protein required for cytochrome c oxidase assembly; located in the cytosol and mitochondrial intermembrane space; putative copper metallochaperone that delivers copper to cytochrome c oxidase; contains twin cysteine-x9-cysteine motifs
Localization
Cell Percentages cytoplasm (99%)
Cell Cycle Regulation No
Subcompartmental Group cytoplasm-9

Cox19

Cox19


Nearest neighbours ( genes)

correlation threshold

0.6

1.0

0.80


Biological Process (Gene Ontology)
Molecular Function (Gene Ontology)
Cellular Component (Gene Ontology)

Endocytosis
Temp Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Vph1-GFP)
37℃
RT
Cell Cycle Omics

CYCLoPs (Cox19-GFP)
Gene / Allele Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Sac6-tdTomato)
Gene Images
Gene Images
Images are not yet available
Images are not yet available