Standard name | |
---|---|
Human Ortholog | |
Description | Transmembrane nucleoporin; involved in nuclear pore complex (NPC) distribution, assembly or stabilization; highly conserved across species, orthologous to human TMEM33 and paralogous to Per33p; protein abundance increases in response to DNA replication stress |
ensLOC | DeepLoc | |||||||||||||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Localization | WT1 | WT2 | WT3 | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 | WT1 | WT2 | WT3 | AF100 | AF140 | AF180 |
Cortical Patches | 1 | 1 | 0 | 0 | 1 | 6 | 1 | 2 | 2 | 6 | 1 | 6 | 1 | 2 | 3 | 5 | 4 | 1 | 4 | 0 | 4 | 0 | 2 | 2 |
Bud | 1 | 1 | 3 | 1 | 1 | 4 | 9 | 6 | 7 | 13 | 9 | 7 | 1 | 0 | 0 | 0 | 1 | 0 | 2 | 3 | 1 | 1 | 2 | 3 |
Bud Neck | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 0 | 0 | 0 | 0 | 0 |
Bud Site | 0 | 0 | 0 | 1 | 1 | 4 | 5 | 2 | 1 | 9 | 6 | 9 | 0 | 0 | 0 | 0 | 0 | 0 | – | – | – | – | – | – |
Cell Periphery | 0 | 0 | 4 | 0 | 2 | 3 | 3 | 1 | 2 | 3 | 2 | 4 | 0 | 7 | 6 | 4 | 2 | 3 | 0 | 0 | 0 | 0 | 0 | 0 |
Cytoplasm | 11 | 3 | 7 | 11 | 17 | 27 | 27 | 22 | 13 | 20 | 20 | 23 | 3 | 17 | 23 | 6 | 12 | 7 | 0 | 1 | 1 | 1 | 1 | 3 |
Endoplasmic Reticulum | 20 | 11 | 5 | 8 | 14 | 100 | 25 | 39 | 26 | 30 | 45 | 53 | 16 | 26 | 40 | 4 | 1 | 2 | 36 | 23 | 20 | 3 | 7 | 17 |
Endosome | 1 | 3 | 6 | 6 | 14 | 0 | 13 | 12 | 4 | 14 | 2 | 3 | 2 | 7 | 13 | 0 | 2 | 0 | 2 | 3 | 5 | 2 | 22 | 15 |
Golgi | 0 | 1 | 0 | 0 | 0 | 0 | 1 | 2 | 0 | 0 | 1 | 4 | 0 | 3 | 11 | 21 | 19 | 14 | 0 | 2 | 1 | 1 | 6 | 13 |
Mitochondria | 27 | 12 | 125 | 105 | 166 | 223 | 312 | 380 | 282 | 334 | 355 | 455 | 2 | 1 | 2 | 7 | 3 | 4 | 2 | 3 | 15 | 5 | 19 | 26 |
Nucleus | 10 | 4 | 8 | 15 | 8 | 18 | 35 | 25 | 18 | 30 | 40 | 35 | 5 | 4 | 2 | 0 | 1 | 0 | 0 | 3 | 1 | 0 | 0 | 1 |
Nuclear Periphery | 58 | 75 | 89 | 96 | 117 | 109 | 144 | 136 | 134 | 179 | 143 | 119 | 34 | 54 | 52 | 8 | 5 | 9 | 77 | 107 | 177 | 54 | 88 | 65 |
Nucleolus | 3 | 0 | 7 | 3 | 7 | 5 | 18 | 45 | 19 | 23 | 25 | 31 | 0 | 1 | 1 | 1 | 2 | 1 | 0 | 0 | 0 | 0 | 0 | 0 |
Peroxisomes | 0 | 0 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 3 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 0 | 0 | 0 |
SpindlePole | 0 | 0 | 0 | 0 | 2 | 2 | 2 | 1 | 0 | 3 | 2 | 2 | 1 | 1 | 1 | 0 | 0 | 0 | 0 | 0 | 3 | 1 | 4 | 2 |
Vac/Vac Membrane | 13 | 86 | 62 | 53 | 91 | 52 | 121 | 123 | 62 | 133 | 81 | 54 | 35 | 65 | 45 | 19 | 31 | 22 | 2 | 13 | 4 | 3 | 12 | 11 |
Unique Cell Count | 111 | 142 | 216 | 208 | 315 | 386 | 511 | 611 | 400 | 574 | 520 | 606 | 82 | 150 | 146 | 64 | 71 | 57 | 135 | 162 | 245 | 76 | 172 | 167 |
Labelled Cell Count | 145 | 197 | 317 | 299 | 441 | 553 | 716 | 796 | 570 | 800 | 733 | 805 | 100 | 188 | 199 | 75 | 83 | 63 | 135 | 162 | 245 | 76 | 172 | 167 |
Screen | WT1 | WT2 | WT3 | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 | AF100 | AF140 | AF180 |
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Mean Cell GFP Intensity (1e-4) | 8.9 | 9.4 | 5.7 | 5.8 | 5.7 | 5.9 | 5.8 | 6.2 | 5.8 | 6.2 | 5.9 | 6.2 | 7.7 | 10.2 | 11.0 | 16.5 | 15.8 | 15.9 | 7.0 | 8.1 | 10.3 |
Std Deviation (1e-4) | 2.4 | 2.6 | 1.5 | 1.9 | 1.5 | 1.5 | 1.8 | 1.8 | 1.8 | 1.9 | 1.9 | 2.3 | 1.9 | 3.1 | 3.0 | 4.7 | 5.1 | 3.9 | 1.6 | 2.8 | 7.0 |
Intensity Change (Log2) | – | – | – | 0.04 | 0.01 | 0.05 | 0.03 | 0.12 | 0.04 | 0.13 | 0.06 | 0.14 | 0.45 | 0.84 | 0.95 | 1.54 | 1.47 | 1.49 | 0.3 | 0.51 | 0.85 |
Localization | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 |
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Cortical Patches | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Bud | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Bud Neck | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Bud Site | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Cell Periphery | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Cytoplasm | 1.0 | 1.2 | 1.9 | 1.2 | 0.2 | 0 | 0.2 | 0.4 | 0.4 | 0 | 3.1 | 4.2 | 0 | 0 | 0 |
Endoplasmic Reticulum | 0.9 | 1.3 | 7.3 | 1.6 | 2.3 | 2.3 | 1.8 | 3.1 | 3.2 | 5.2 | 5.1 | 7.1 | 0 | 0 | 0 |
Endosome | 0.1 | 1.0 | 0 | -0.2 | 0 | 0 | -0.3 | 0 | 0 | 0 | 1.0 | 2.6 | 0 | 0 | 0 |
Golgi | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Mitochondria | -1.5 | -1.2 | 0 | 0.8 | 1.1 | 3.2 | 0.1 | 2.7 | 4.8 | -8.6 | -11.3 | -11.1 | -6.6 | -7.9 | -6.8 |
Nucleus | 1.6 | -0.8 | 0.6 | 1.6 | 0.3 | 0.5 | 0.9 | 2.0 | 1.2 | 0 | 0 | 0 | 0 | 0 | 0 |
Nuclear Periphery | 1.0 | -0.9 | -3.2 | -3.4 | -5.4 | -1.9 | -2.7 | -3.6 | -6.3 | 0 | -1.0 | -1.1 | -4.2 | -5.3 | -3.6 |
Nucleolus | 0 | -0.7 | 0 | 0.2 | 2.1 | 0.9 | 0.5 | 0.9 | 1.1 | 0 | 0 | 0 | 0 | 0 | 0 |
Peroxisomes | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
SpindlePole | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Vacuole | -0.7 | 0 | -4.6 | -1.4 | -2.6 | -3.9 | -1.6 | -4.1 | -7.2 | 2.3 | 2.9 | 0.4 | 0.2 | 2.3 | 1.4 |
R1 | R2 | R3 | ||||||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | |
Concentration | 7.5189 | 6.1778 | 6.1979 | 5.5929 | 6.1834 | 6.3389 | 9.1808 | 7.8834 | 8.3359 | 8.0758 | 7.0829 | 7.9877 | 7.752 | 6.1504 | 6.3599 | 4.7006 | 4.815 | 5.5758 |
Actin | 0.0018 | 0.0004 | 0.0003 | 0.0006 | 0.0002 | 0.0004 | 0.0057 | 0.0174 | 0.0005 | 0.0011 | 0.0003 | 0.0002 | 0.001 | 0.0005 | 0.0009 | 0.001 | 0.0071 | 0.0003 |
Bud | 0 | 0 | 0 | 0 | 0 | 0.0004 | 0 | 0.0003 | 0 | 0.0001 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Bud Neck | 0.0001 | 0.0002 | 0.0002 | 0.0001 | 0.0001 | 0.0005 | 0.0001 | 0.0003 | 0 | 0.0015 | 0.0001 | 0.0001 | 0.0001 | 0.0001 | 0.0001 | 0.0001 | 0.0002 | 0.0001 |
Bud Periphery | 0 | 0 | 0 | 0 | 0 | 0.0002 | 0.0001 | 0.0003 | 0.0001 | 0.0003 | 0.0001 | 0 | 0 | 0.0001 | 0.0001 | 0.0001 | 0.0002 | 0 |
Bud Site | 0.0001 | 0.0005 | 0.0001 | 0 | 0 | 0.0001 | 0.0001 | 0.0055 | 0 | 0.0025 | 0 | 0 | 0.0001 | 0 | 0 | 0 | 0 | 0 |
Cell Periphery | 0.0001 | 0.0001 | 0.0001 | 0 | 0 | 0.0001 | 0.0001 | 0.0009 | 0.0001 | 0.0006 | 0.0002 | 0 | 0.0001 | 0.0002 | 0.0002 | 0.0001 | 0.0005 | 0.0001 |
Cytoplasm | 0.0017 | 0.0151 | 0.0069 | 0.001 | 0.0017 | 0.0045 | 0.0015 | 0.0008 | 0.0005 | 0.0046 | 0.0004 | 0.0017 | 0.0037 | 0.001 | 0.0007 | 0.0005 | 0.0006 | 0.0006 |
Cytoplasmic Foci | 0.0015 | 0.001 | 0.0003 | 0.0003 | 0.0002 | 0.0007 | 0.0019 | 0.0007 | 0.0002 | 0.0006 | 0.0002 | 0.0007 | 0.0024 | 0.0001 | 0.0001 | 0.0004 | 0.0006 | 0.0002 |
Eisosomes | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0.0001 | 0 | 0.0001 | 0 | 0 | 0 | 0 | 0 | 0 | 0.0001 | 0 |
Endoplasmic Reticulum | 0.1223 | 0.2087 | 0.1872 | 0.1051 | 0.1789 | 0.1579 | 0.2033 | 0.2173 | 0.2857 | 0.2455 | 0.2769 | 0.2142 | 0.1779 | 0.3243 | 0.2961 | 0.1325 | 0.1999 | 0.2547 |
Endosome | 0.0064 | 0.0124 | 0.008 | 0.0049 | 0.0082 | 0.0053 | 0.0064 | 0.003 | 0.0039 | 0.0105 | 0.0068 | 0.0034 | 0.006 | 0.0036 | 0.0038 | 0.0073 | 0.0175 | 0.0035 |
Golgi | 0.0009 | 0.0004 | 0.0004 | 0.0004 | 0.0003 | 0.0003 | 0.0009 | 0.0028 | 0.0004 | 0.0008 | 0.0012 | 0.0002 | 0.0008 | 0.0002 | 0.0005 | 0.0012 | 0.0052 | 0.0005 |
Lipid Particles | 0.0023 | 0.0038 | 0.0015 | 0.0008 | 0.0026 | 0.0015 | 0.004 | 0.0163 | 0.0031 | 0.0034 | 0.0075 | 0.0013 | 0.0037 | 0.0016 | 0.0022 | 0.0091 | 0.0144 | 0.0027 |
Mitochondria | 0.0008 | 0.0003 | 0.0007 | 0.0009 | 0.0006 | 0.001 | 0.0009 | 0.0022 | 0.001 | 0.0013 | 0.0007 | 0.0004 | 0.0005 | 0.0008 | 0.0006 | 0.0013 | 0.0028 | 0.0007 |
None | 0.0005 | 0.0005 | 0.0003 | 0.0002 | 0.0003 | 0.0004 | 0.0005 | 0.0001 | 0.0013 | 0.0043 | 0.0002 | 0.0042 | 0.0007 | 0.0001 | 0.0002 | 0.0003 | 0.0005 | 0.0001 |
Nuclear Periphery | 0.8089 | 0.6803 | 0.7148 | 0.8436 | 0.7611 | 0.7784 | 0.7333 | 0.69 | 0.6622 | 0.6665 | 0.6778 | 0.7456 | 0.7643 | 0.6271 | 0.6571 | 0.8026 | 0.7203 | 0.709 |
Nucleolus | 0.0001 | 0.0007 | 0.0005 | 0.0001 | 0.0002 | 0.0009 | 0.0002 | 0.0001 | 0.0001 | 0.0056 | 0.0001 | 0.0004 | 0.0001 | 0.0002 | 0 | 0.0001 | 0.0002 | 0.0012 |
Nucleus | 0.025 | 0.0497 | 0.0466 | 0.0179 | 0.0176 | 0.029 | 0.0075 | 0.0036 | 0.0043 | 0.017 | 0.0031 | 0.0059 | 0.0103 | 0.0128 | 0.0074 | 0.0061 | 0.0045 | 0.007 |
Peroxisomes | 0.0008 | 0 | 0 | 0 | 0 | 0 | 0.0008 | 0 | 0.0001 | 0.0001 | 0 | 0 | 0.0021 | 0 | 0 | 0.0001 | 0.0001 | 0 |
Punctate Nuclear | 0.0038 | 0.0035 | 0.003 | 0.004 | 0.0014 | 0.0013 | 0.0063 | 0.0079 | 0.0013 | 0.0039 | 0.0013 | 0.0037 | 0.0011 | 0.0007 | 0.0004 | 0.0013 | 0.0022 | 0.002 |
Vacuole | 0.0035 | 0.0095 | 0.0058 | 0.0019 | 0.0041 | 0.0035 | 0.003 | 0.0035 | 0.0033 | 0.0061 | 0.003 | 0.0019 | 0.0033 | 0.005 | 0.0034 | 0.0035 | 0.0042 | 0.0018 |
Vacuole Periphery | 0.0196 | 0.013 | 0.0232 | 0.0181 | 0.0224 | 0.0136 | 0.0233 | 0.0271 | 0.032 | 0.0237 | 0.0201 | 0.0158 | 0.0218 | 0.0213 | 0.0259 | 0.0323 | 0.0188 | 0.0154 |
R1 | R2 | |||||||||
---|---|---|---|---|---|---|---|---|---|---|
G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | |
Gene Expression | 53.8566 | 36.402 | 25.2691 | 48.8575 | 28.1013 | 60.3911 | 44.2266 | 54.4912 | 50.4044 | 61.0369 |
Translational Efficiency | 1.0842 | 1.3263 | 1.8845 | 0.8312 | 1.8723 | 1.544 | 1.4221 | 1.1263 | 1.6745 | 1.2148 |
Dataset | Hit |
---|---|
Protein Concentration | ✘ |
Protein Localization | ✘ |
Gene Expression | ✘ |
Translational Efficiency | ✘ |
R1 | R2 | R1 & R2 | |||||||||
---|---|---|---|---|---|---|---|---|---|---|---|
WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 |
1347 | 1236 | 2739 | 1437 | 1791 | 907 | 2483 | 1787 | 3138 | 2143 | 5222 | 3224 |
R1 | R2 | R1 & R2 | ||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|
WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 | |
Mean | 922.85 | 905.70 | 1644.53 | 1780.17 | 897.55 | 860.63 | 1449.23 | 1378.59 | 908.41 | 886.62 | 1551.67 | 1557.58 |
Standard Deviation | 158.97 | 187.52 | 341.51 | 456.51 | 202.00 | 152.33 | 291.67 | 441.57 | 185.18 | 174.92 | 333.37 | 490.72 |
Intensity Change Log 2 | — | -0.027063 | 0.833507 | 0.947847 | — | -0.060599 | 0.691222 | 0.619129 | — | -0.043501 | 0.765106 | 0.795103 |
R1 | R2 | R1 & R2 | ||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|
WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 | |
Actin | 0.000117 | 0.000325 | 0.000218 | 0.001553 | 0.000354 | 0.000208 | 0.000159 | 0.001134 | 0.000253 | 0.000275 | 0.000190 | 0.001321 |
Bud Neck | 0.000121 | 0.000815 | 0.001474 | 0.006683 | 0.000595 | 0.000556 | 0.000568 | 0.002535 | 0.000392 | 0.000705 | 0.001043 | 0.004384 |
Bud Site | 0.000161 | 0.000514 | 0.001306 | 0.017322 | 0.001691 | 0.000799 | 0.000763 | 0.009279 | 0.001034 | 0.000635 | 0.001048 | 0.012864 |
Cell Periphery | 0.000134 | 0.000145 | 0.000471 | 0.005873 | 0.000365 | 0.000100 | 0.000335 | 0.002402 | 0.000266 | 0.000126 | 0.000406 | 0.003949 |
Cytoplasm | 0.004037 | 0.014061 | 0.007290 | 0.009649 | 0.017227 | 0.014102 | 0.004083 | 0.017617 | 0.011565 | 0.014078 | 0.005765 | 0.014066 |
Cytoplasmic Foci | 0.000690 | 0.007329 | 0.000254 | 0.003509 | 0.006212 | 0.007382 | 0.000183 | 0.003827 | 0.003841 | 0.007352 | 0.000220 | 0.003686 |
Eisosomes | 0.000004 | 0.000012 | 0.000005 | 0.000097 | 0.000014 | 0.000013 | 0.000005 | 0.000192 | 0.000010 | 0.000012 | 0.000005 | 0.000149 |
Endoplasmic Reticulum | 0.101565* | 0.077018 | 0.658915* | 0.637509* | 0.140867* | 0.050367 | 0.583515* | 0.492503* | 0.123996* | 0.065738 | 0.623063* | 0.557135* |
Endosome | 0.010202 | 0.016543 | 0.028220 | 0.047286 | 0.024393 | 0.011723 | 0.029757 | 0.042783 | 0.018301 | 0.014503 | 0.028951 | 0.044790 |
Golgi | 0.000461 | 0.002467 | 0.000446 | 0.010165 | 0.003743 | 0.001991 | 0.000520 | 0.006972 | 0.002334 | 0.002266 | 0.000481 | 0.008395 |
Lipid Particles | 0.019108 | 0.034019 | 0.007435 | 0.075557 | 0.033939 | 0.053510 | 0.006990 | 0.028495 | 0.027573 | 0.042269 | 0.007223 | 0.049471 |
Mitochondria | 0.004445 | 0.007101 | 0.000738 | 0.000789 | 0.006497 | 0.011249 | 0.000581 | 0.001850 | 0.005616 | 0.008856 | 0.000664 | 0.001377 |
Mitotic Spindle | 0.001227 | 0.004590 | 0.001284 | 0.015551 | 0.001827 | 0.008183 | 0.001938 | 0.006972 | 0.001570 | 0.006111 | 0.001595 | 0.010796 |
None | 0.001571 | 0.005357 | 0.002070 | 0.001314 | 0.010835 | 0.003344 | 0.001693 | 0.006846 | 0.006858 | 0.004505 | 0.001891 | 0.004380 |
Nuclear Periphery | 0.315847* | 0.255359* | 0.099207 | 0.018129 | 0.176837* | 0.278848* | 0.127587* | 0.081054 | 0.236508* | 0.265300* | 0.112702* | 0.053007 |
Nuclear Periphery Foci | 0.531168* | 0.546560* | 0.186028 | 0.130155 | 0.550733* | 0.527622* | 0.237980* | 0.274050* | 0.542335* | 0.538545* | 0.210731* | 0.209913* |
Nucleolus | 0.000815 | 0.004502 | 0.000018 | 0.000047 | 0.004357 | 0.004566 | 0.000020 | 0.000739 | 0.002837 | 0.004529 | 0.000019 | 0.000431 |
Nucleus | 0.002702 | 0.012460 | 0.001153 | 0.001146 | 0.009886 | 0.011681 | 0.000547 | 0.005064 | 0.006802 | 0.012131 | 0.000865 | 0.003318 |
Peroxisomes | 0.000159 | 0.000885 | 0.000008 | 0.000052 | 0.000558 | 0.001464 | 0.000007 | 0.000091 | 0.000387 | 0.001130 | 0.000007 | 0.000074 |
Vacuole | 0.003288 | 0.006351 | 0.003142 | 0.015397 | 0.005517 | 0.007138 | 0.002430 | 0.013466 | 0.004560 | 0.006684 | 0.002804 | 0.014326 |
Vacuole Periphery | 0.002178 | 0.003586 | 0.000316 | 0.002217 | 0.003553 | 0.005155 | 0.000339 | 0.002129 | 0.002963 | 0.004250 | 0.000327 | 0.002168 |
R1 | R2 | R1 & R2 | |||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
UBP2_WT | UBP14_WT | UBP2UBP14_WT | UBP2UBP14_UBP2 | UBP2UBP14_UBP14 | UBP2_WT | UBP14_WT | UBP2UBP14_WT | UBP2UBP14_UBP2 | UBP2UBP14_UBP14 | UBP2_WT | UBP14_WT | UBP2UBP14_WT | UBP2UBP14_UBP2 | UBP2UBP14_UBP14 | |
Actin | -2.91 | -2.30 | -6.16 | -5.36 | -5.58 | 2.18 | 1.10 | -4.11 | -4.76 | -4.44 | -0.32 | -0.00 | -7.07 | -6.99 | -6.99 |
Bud Neck | -2.51 | -6.72 | -10.38 | -8.82 | -7.82 | 0.06 | -0.58 | -7.83 | -6.82 | -7.34 | -1.81 | -5.96 | -12.66 | -10.64 | -9.84 |
Bud Site | -3.35 | -4.01 | -8.63 | -8.47 | -7.90 | 1.55 | 1.13 | -5.82 | -6.28 | -6.16 | 1.40 | -1.03 | -10.32 | -10.61 | -9.97 |
Cell Periphery | -0.46 | -8.37 | -6.68 | -6.66 | -6.23 | 6.46 | 0.09 | -7.02 | -7.95 | -6.80 | 5.50 | -3.25 | -8.88 | -9.22 | -8.42 |
Cytoplasm | -5.12 | -3.92 | -4.87 | 1.43 | -2.37 | 0.91 | 7.66 | -0.51 | -1.36 | -8.57 | -1.69 | 5.07 | -2.45 | -0.37 | -8.07 |
Cytoplasmic Foci | -5.91 | 2.95 | -6.91 | 3.14 | -8.20 | -1.00 | 8.38 | 2.81 | 2.95 | -7.96 | -3.90 | 8.62 | 0.25 | 4.24 | -11.21 |
Eisosomes | -5.35 | -1.40 | -6.37 | -5.82 | -6.28 | 0.34 | 4.37 | -1.37 | -1.38 | -1.44 | -2.18 | 3.74 | -1.97 | -1.93 | -2.03 |
Endoplasmic Reticulum | 3.72 | -66.63* | -47.32* | -51.53* | 2.82 | 13.85 | -48.84* | -32.45 | -43.68* | 8.00 | 12.23 | -80.30 | -54.34 | -64.98* | 8.67 |
Endosome | -4.18 | -16.82 | -20.41 | -14.45 | -10.24 | 7.05 | -3.54 | -9.26 | -15.42 | -7.14 | 3.04 | -10.87 | -19.17 | -20.51 | -12.16 |
Golgi | -4.38 | 0.07 | -7.76 | -5.74 | -7.79 | 2.11 | 5.05 | -3.27 | -5.29 | -8.39 | 0.12 | 5.07 | -7.69 | -7.80 | -11.32 |
Lipid Particles | -4.67 | 6.38 | -12.15 | -8.14 | -15.43 | -4.25 | 12.38 | 1.72 | 5.35 | -9.97 | -5.38 | 13.79 | -8.31 | -2.34 | -18.07 |
Mitochondria | -2.52 | 4.40 | 4.30 | 8.88 | -0.36 | -2.89 | 8.14 | 5.79 | 6.14 | -3.98 | -3.56 | 9.00 | 7.25 | 9.65 | -3.71 |
Mitotic Spindle | -3.97 | -0.33 | -7.30 | -5.58 | -7.24 | -3.50 | -0.43 | -4.81 | 0.21 | -4.19 | -5.05 | -0.42 | -8.72 | -4.05 | -8.38 |
None | -4.19 | -1.43 | 0.58 | 4.26 | 1.65 | 4.97 | 6.62 | 2.22 | -2.39 | -3.98 | 2.18 | 6.18 | 2.39 | 0.39 | -3.36 |
Nuclear Periphery | 6.57 | 29.70* | 43.92* | 35.10* | 23.55 | -10.95 | 8.09 | 16.27 | 22.94* | 9.29 | -4.30 | 25.87 | 39.63 | 38.71* | 18.77 |
Nuclear Periphery Foci | -1.27 | 38.44* | 41.38* | 41.46* | 7.36 | 1.93 | 34.15* | 26.60* | 20.58* | -3.82 | 0.56 | 51.61 | 45.37 | 40.82* | 0.12 |
Nucleolus | -4.75 | 3.41 | 3.20 | 5.95 | -1.62 | -0.17 | 6.52 | 5.36 | 4.10 | -4.68 | -2.35 | 7.13 | 5.99 | 6.97 | -4.85 |
Nucleus | -5.07 | 1.66 | 1.25 | 5.81 | -0.53 | -0.97 | 7.15 | 3.01 | 3.54 | -5.29 | -3.67 | 7.07 | 3.33 | 6.31 | -4.90 |
Peroxisomes | -3.32 | 6.76 | 4.53 | 3.81 | -6.15 | -1.73 | 6.21 | 5.20 | 2.67 | -4.28 | -2.85 | 7.29 | 5.94 | 4.16 | -5.84 |
Vacuole | -3.49 | -0.62 | -10.55 | -7.65 | -10.61 | -1.23 | 4.91 | -6.70 | -4.34 | -9.51 | -2.80 | 3.33 | -11.79 | -8.36 | -13.99 |
Vacuole Periphery | -3.07 | 7.40 | -0.15 | 2.55 | -5.34 | -1.45 | 8.64 | 3.15 | 2.80 | -7.55 | -2.35 | 11.05 | 2.42 | 3.83 | -8.96 |
Temp | Actin Patch (Sac6-tdTomato) | Cortical Patch (Sla1-GFP) | Late Endosome (Snf7-GFP) | Vacuole (Vph1-GFP) |
---|---|---|---|---|
37℃ | ||||
RT |
Gene / Allele | Actin Patch (Sac6-tdTomato) | Cortical Patch (Sla1-GFP) | Late Endosome (Snf7-GFP) | Vacuole (Sac6-tdTomato) |
---|
Gene | Images |
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Gene | Images |
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