Standard name
Human Ortholog
Description Transmembrane nucleoporin; involved in nuclear pore complex (NPC) distribution, assembly or stabilization; highly conserved across species, orthologous to human TMEM33 and paralogous to Per33p; protein abundance increases in response to DNA replication stress
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Micrographs

ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0.08 0.06 0 0 0 0 0 0 0
Bud 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 – – – – – –
Cell Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0.05 0 0.06 0 0.05 0 0 0 0 0 0
Cytoplasm 0.1 0 0 0.05 0.05 0.07 0.05 0 0 0 0 0 0 0.11 0.16 0.09 0.17 0.12 0 0 0 0 0 0
Endoplasmic Reticulum 0.18 0.08 0 0 0 0.26 0.05 0.06 0.07 0.05 0.09 0.09 0.2 0.17 0.27 0.06 0 0 0.27 0.14 0.09 0.05 0 0.11
Endosome 0 0 0 0 0 0 0 0 0 0 0 0 0 0.05 0.09 0 0 0 0 0 0 0 0.13 0.09
Golgi 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0.08 0.33 0.27 0.25 0 0 0 0 0 0.08
Mitochondria 0.24 0.08 0.58 0.5 0.53 0.58 0.61 0.62 0.7 0.58 0.68 0.75 0 0 0 0.11 0 0.07 0 0 0.06 0.07 0.11 0.16
Nucleus 0.09 0 0 0.07 0 0.05 0.07 0 0 0.05 0.08 0.06 0.06 0 0 0 0 0 0 0 0 0 0 0
Nuclear Periphery 0.52 0.53 0.41 0.46 0.37 0.28 0.28 0.22 0.34 0.31 0.28 0.2 0.41 0.36 0.36 0.12 0.07 0.16 0.57 0.66 0.72 0.72 0.52 0.4
Nucleolus 0 0 0 0 0 0 0 0.07 0.05 0 0.05 0.05 0 0 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Vac/Vac Membrane 0.12 0.61 0.29 0.25 0.29 0.13 0.24 0.2 0.15 0.23 0.16 0.09 0.43 0.43 0.31 0.3 0.44 0.39 0 0.08 0 0 0.07 0.07
ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 1 1 0 0 1 6 1 2 2 6 1 6 1 2 3 5 4 1 4 0 4 0 2 2
Bud 1 1 3 1 1 4 9 6 7 13 9 7 1 0 0 0 1 0 2 3 1 1 2 3
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 1 0 0 0 0 0
Bud Site 0 0 0 1 1 4 5 2 1 9 6 9 0 0 0 0 0 0 – – – – – –
Cell Periphery 0 0 4 0 2 3 3 1 2 3 2 4 0 7 6 4 2 3 0 0 0 0 0 0
Cytoplasm 11 3 7 11 17 27 27 22 13 20 20 23 3 17 23 6 12 7 0 1 1 1 1 3
Endoplasmic Reticulum 20 11 5 8 14 100 25 39 26 30 45 53 16 26 40 4 1 2 36 23 20 3 7 17
Endosome 1 3 6 6 14 0 13 12 4 14 2 3 2 7 13 0 2 0 2 3 5 2 22 15
Golgi 0 1 0 0 0 0 1 2 0 0 1 4 0 3 11 21 19 14 0 2 1 1 6 13
Mitochondria 27 12 125 105 166 223 312 380 282 334 355 455 2 1 2 7 3 4 2 3 15 5 19 26
Nucleus 10 4 8 15 8 18 35 25 18 30 40 35 5 4 2 0 1 0 0 3 1 0 0 1
Nuclear Periphery 58 75 89 96 117 109 144 136 134 179 143 119 34 54 52 8 5 9 77 107 177 54 88 65
Nucleolus 3 0 7 3 7 5 18 45 19 23 25 31 0 1 1 1 2 1 0 0 0 0 0 0
Peroxisomes 0 0 1 0 0 0 0 0 0 3 1 0 0 0 0 0 0 0 0 0 1 0 0 0
SpindlePole 0 0 0 0 2 2 2 1 0 3 2 2 1 1 1 0 0 0 0 0 3 1 4 2
Vac/Vac Membrane 13 86 62 53 91 52 121 123 62 133 81 54 35 65 45 19 31 22 2 13 4 3 12 11
Unique Cell Count 111 142 216 208 315 386 511 611 400 574 520 606 82 150 146 64 71 57 135 162 245 76 172 167
Labelled Cell Count 145 197 317 299 441 553 716 796 570 800 733 805 100 188 199 75 83 63 135 162 245 76 172 167


Endoplasmic Reticulum

(Huh et al. Nature 2003 425:686)
Screen WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 AF100 AF140 AF180
Mean Cell GFP Intensity (1e-4) 8.9 9.4 5.7 5.8 5.7 5.9 5.8 6.2 5.8 6.2 5.9 6.2 7.7 10.2 11.0 16.5 15.8 15.9 7.0 8.1 10.3
Std Deviation (1e-4) 2.4 2.6 1.5 1.9 1.5 1.5 1.8 1.8 1.8 1.9 1.9 2.3 1.9 3.1 3.0 4.7 5.1 3.9 1.6 2.8 7.0
Intensity Change (Log2) – – – 0.04 0.01 0.05 0.03 0.12 0.04 0.13 0.06 0.14 0.45 0.84 0.95 1.54 1.47 1.49 0.3 0.51 0.85


Localization RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3
Cortical Patches 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cell Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cytoplasm 1.0 1.2 1.9 1.2 0.2 0 0.2 0.4 0.4 0 3.1 4.2 0 0 0
Endoplasmic Reticulum 0.9 1.3 7.3 1.6 2.3 2.3 1.8 3.1 3.2 5.2 5.1 7.1 0 0 0
Endosome 0.1 1.0 0 -0.2 0 0 -0.3 0 0 0 1.0 2.6 0 0 0
Golgi 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Mitochondria -1.5 -1.2 0 0.8 1.1 3.2 0.1 2.7 4.8 -8.6 -11.3 -11.1 -6.6 -7.9 -6.8
Nucleus 1.6 -0.8 0.6 1.6 0.3 0.5 0.9 2.0 1.2 0 0 0 0 0 0
Nuclear Periphery 1.0 -0.9 -3.2 -3.4 -5.4 -1.9 -2.7 -3.6 -6.3 0 -1.0 -1.1 -4.2 -5.3 -3.6
Nucleolus 0 -0.7 0 0.2 2.1 0.9 0.5 0.9 1.1 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Vacuole -0.7 0 -4.6 -1.4 -2.6 -3.9 -1.6 -4.1 -7.2 2.3 2.9 0.4 0.2 2.3 1.4

Images

Circle plot not available

Protein Concentration and Protein Localization Data

R1 R2 R3
G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase
Concentration 7.5189 6.1778 6.1979 5.5929 6.1834 6.3389 9.1808 7.8834 8.3359 8.0758 7.0829 7.9877 7.752 6.1504 6.3599 4.7006 4.815 5.5758
Actin 0.0018 0.0004 0.0003 0.0006 0.0002 0.0004 0.0057 0.0174 0.0005 0.0011 0.0003 0.0002 0.001 0.0005 0.0009 0.001 0.0071 0.0003
Bud 0 0 0 0 0 0.0004 0 0.0003 0 0.0001 0 0 0 0 0 0 0 0
Bud Neck 0.0001 0.0002 0.0002 0.0001 0.0001 0.0005 0.0001 0.0003 0 0.0015 0.0001 0.0001 0.0001 0.0001 0.0001 0.0001 0.0002 0.0001
Bud Periphery 0 0 0 0 0 0.0002 0.0001 0.0003 0.0001 0.0003 0.0001 0 0 0.0001 0.0001 0.0001 0.0002 0
Bud Site 0.0001 0.0005 0.0001 0 0 0.0001 0.0001 0.0055 0 0.0025 0 0 0.0001 0 0 0 0 0
Cell Periphery 0.0001 0.0001 0.0001 0 0 0.0001 0.0001 0.0009 0.0001 0.0006 0.0002 0 0.0001 0.0002 0.0002 0.0001 0.0005 0.0001
Cytoplasm 0.0017 0.0151 0.0069 0.001 0.0017 0.0045 0.0015 0.0008 0.0005 0.0046 0.0004 0.0017 0.0037 0.001 0.0007 0.0005 0.0006 0.0006
Cytoplasmic Foci 0.0015 0.001 0.0003 0.0003 0.0002 0.0007 0.0019 0.0007 0.0002 0.0006 0.0002 0.0007 0.0024 0.0001 0.0001 0.0004 0.0006 0.0002
Eisosomes 0 0 0 0 0 0 0 0.0001 0 0.0001 0 0 0 0 0 0 0.0001 0
Endoplasmic Reticulum 0.1223 0.2087 0.1872 0.1051 0.1789 0.1579 0.2033 0.2173 0.2857 0.2455 0.2769 0.2142 0.1779 0.3243 0.2961 0.1325 0.1999 0.2547
Endosome 0.0064 0.0124 0.008 0.0049 0.0082 0.0053 0.0064 0.003 0.0039 0.0105 0.0068 0.0034 0.006 0.0036 0.0038 0.0073 0.0175 0.0035
Golgi 0.0009 0.0004 0.0004 0.0004 0.0003 0.0003 0.0009 0.0028 0.0004 0.0008 0.0012 0.0002 0.0008 0.0002 0.0005 0.0012 0.0052 0.0005
Lipid Particles 0.0023 0.0038 0.0015 0.0008 0.0026 0.0015 0.004 0.0163 0.0031 0.0034 0.0075 0.0013 0.0037 0.0016 0.0022 0.0091 0.0144 0.0027
Mitochondria 0.0008 0.0003 0.0007 0.0009 0.0006 0.001 0.0009 0.0022 0.001 0.0013 0.0007 0.0004 0.0005 0.0008 0.0006 0.0013 0.0028 0.0007
None 0.0005 0.0005 0.0003 0.0002 0.0003 0.0004 0.0005 0.0001 0.0013 0.0043 0.0002 0.0042 0.0007 0.0001 0.0002 0.0003 0.0005 0.0001
Nuclear Periphery 0.8089 0.6803 0.7148 0.8436 0.7611 0.7784 0.7333 0.69 0.6622 0.6665 0.6778 0.7456 0.7643 0.6271 0.6571 0.8026 0.7203 0.709
Nucleolus 0.0001 0.0007 0.0005 0.0001 0.0002 0.0009 0.0002 0.0001 0.0001 0.0056 0.0001 0.0004 0.0001 0.0002 0 0.0001 0.0002 0.0012
Nucleus 0.025 0.0497 0.0466 0.0179 0.0176 0.029 0.0075 0.0036 0.0043 0.017 0.0031 0.0059 0.0103 0.0128 0.0074 0.0061 0.0045 0.007
Peroxisomes 0.0008 0 0 0 0 0 0.0008 0 0.0001 0.0001 0 0 0.0021 0 0 0.0001 0.0001 0
Punctate Nuclear 0.0038 0.0035 0.003 0.004 0.0014 0.0013 0.0063 0.0079 0.0013 0.0039 0.0013 0.0037 0.0011 0.0007 0.0004 0.0013 0.0022 0.002
Vacuole 0.0035 0.0095 0.0058 0.0019 0.0041 0.0035 0.003 0.0035 0.0033 0.0061 0.003 0.0019 0.0033 0.005 0.0034 0.0035 0.0042 0.0018
Vacuole Periphery 0.0196 0.013 0.0232 0.0181 0.0224 0.0136 0.0233 0.0271 0.032 0.0237 0.0201 0.0158 0.0218 0.0213 0.0259 0.0323 0.0188 0.0154

Sequencing Data

R1 R2
G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Post-START S/G2 Metaphase Anaphase Telophase
Gene Expression 53.8566 36.402 25.2691 48.8575 28.1013 60.3911 44.2266 54.4912 50.4044 61.0369
Translational Efficiency 1.0842 1.3263 1.8845 0.8312 1.8723 1.544 1.4221 1.1263 1.6745 1.2148

Hit Data

Dataset Hit
Protein Concentration ✘
Protein Localization ✘
Gene Expression ✘
Translational Efficiency ✘
Standard name
Human Ortholog
Description Transmembrane nucleoporin; involved in nuclear pore complex (NPC) distribution, assembly or stabilization; highly conserved across species, orthologous to human TMEM33 and paralogous to Per33p; protein abundance increases in response to DNA replication stress
Localization
Cell Percentages nuclear periphery (65%)
Cell Cycle Regulation No
Subcompartmental Group N/A

Pom33

Pom33


Nearest neighbours ( genes)

correlation threshold

0.6

1.0

0.80


Biological Process (Gene Ontology)
Molecular Function (Gene Ontology)
Cellular Component (Gene Ontology)

Endocytosis
Temp Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Vph1-GFP)
37℃
RT
Cell Cycle Omics

CYCLoPs (Pom33-GFP)
Gene / Allele Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Sac6-tdTomato)
Gene Images
Gene Images
Images are not yet available
Images are not yet available