ORF
Human Ortholog
Description Protein of unknown function; may interact with ribosomes, based on co-purification experiments; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm; YLL032C is not an essential gene
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Micrographs

ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 – – – – – –
Cell Periphery 0 0 0.05 0 0 0 0 0 0 0 0 0.05 0 0 0 0 0 0 0 0 0 0 0 0
Cytoplasm 0.97 0.97 0.96 0.97 0.96 0.9 0.87 0.9 0.72 0.73 0.68 0.75 0.98 0.98 1.0 0.9 0.91 0.9 0.89 0.94 0.89 0.74 0.79 0.82
Endoplasmic Reticulum 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0.09 0.05 0
Endosome 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Golgi 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Mitochondria 0 0 0 0.06 0 0.16 0.22 0.13 0.39 0.37 0.48 0.29 0 0 0 0 0 0 0 0 0 0.08 0.06 0
Nucleus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nuclear Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Vac/Vac Membrane 0 0 0 0 0 0 0 0.05 0 0 0 0 0 0 0 0.07 0.05 0.08 0 0 0 0 0.05 0
ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 3 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 2 1
Bud 0 1 1 0 1 5 4 2 9 11 11 5 2 1 0 1 0 2 0 0 1 3 0 3
Bud Neck 0 0 0 0 0 1 1 0 0 0 0 0 0 0 0 0 0 0 0 1 0 1 2 2
Bud Site 0 0 0 0 0 0 0 1 0 0 0 0 0 0 0 0 0 0 – – – – – –
Cell Periphery 3 10 17 2 5 6 10 7 9 7 10 18 1 4 1 7 7 3 0 0 1 0 0 1
Cytoplasm 115 318 363 255 358 352 463 391 296 304 239 276 298 280 399 180 146 120 107 311 334 112 242 217
Endoplasmic Reticulum 0 5 1 1 0 0 0 0 0 1 0 3 2 1 1 9 3 3 1 2 3 13 16 7
Endosome 0 0 0 1 1 3 1 1 0 1 1 3 1 1 1 3 3 2 1 2 0 0 0 3
Golgi 0 0 0 0 1 0 0 0 0 1 0 0 0 0 0 2 1 1 0 0 1 0 0 1
Mitochondria 2 4 8 17 13 64 115 55 159 152 167 107 4 2 4 5 3 5 1 5 11 11 19 11
Nucleus 0 2 0 0 1 2 8 3 0 3 0 5 1 3 0 1 2 1 1 1 5 0 0 0
Nuclear Periphery 0 0 1 0 0 0 0 0 1 0 4 0 1 0 0 0 0 0 0 0 0 0 1 0
Nucleolus 1 0 0 0 0 0 0 0 0 1 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 1
SpindlePole 0 0 0 0 0 0 2 4 0 0 0 1 0 0 0 1 0 0 0 0 2 0 0 0
Vac/Vac Membrane 0 0 11 2 9 9 4 21 15 17 14 14 0 1 1 13 8 11 2 6 4 6 16 7
Unique Cell Count 118 327 377 262 374 393 532 436 410 414 349 367 303 285 401 200 160 133 121 333 375 152 308 265
Labelled Cell Count 121 343 402 278 389 442 608 485 489 498 446 432 310 293 407 222 173 148 121 333 375 152 308 265


Cytoplasm

(Huh et al. Nature 2003 425:686)
Screen WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 AF100 AF140 AF180
Mean Cell GFP Intensity (1e-4) 6.2 6.0 6.7 5.8 6.5 5.1 5.6 5.8 4.9 4.6 4.6 4.8 5.8 5.9 5.7 9.3 9.3 8.0 5.8 5.6 5.9
Std Deviation (1e-4) 0.8 0.9 1.4 1.1 1.7 1.0 1.3 1.5 1.0 1.0 1.1 0.8 0.9 1.0 0.9 1.8 1.4 1.8 1.5 1.5 1.1
Intensity Change (Log2) – – – -0.2 -0.03 -0.39 -0.26 -0.19 -0.46 -0.52 -0.55 -0.47 -0.19 -0.19 -0.23 0.47 0.47 0.27 -0.19 -0.24 -0.18


Localization RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3
Cortical Patches 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cell Periphery -2.7 -2.6 -2.4 -2.3 -2.4 0 0 0 0 -3.4 -2.3 -3.9 -0.6 -0.1 -1.2
Cytoplasm 0.7 -0.4 -3.6 -4.8 -3.6 -9.1 -8.8 -9.9 -8.3 1.6 1.5 3.2 -3.1 -2.4 -2.7
Endoplasmic Reticulum 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Endosome 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Golgi 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Mitochondria 2.8 1.1 6.7 8.5 5.6 0 0 0 0 -0.8 0 -1.3 0 0 0
Nucleus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nuclear Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Vacuole -1.9 -0.4 -0.5 -2.5 1.4 0.6 0.9 0.8 0.7 0 -2.5 -3.0 2.1 1.2 2.6

Images

Circle plot not available

Protein Concentration and Protein Localization Data

R1 R2 R3
G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase
Concentration 3.6331 4.5265 4.1086 3.921 3.9443 4.2656 1.7862 3.1311 2.569 3.0033 2.5073 2.8468 1.5297 2.3342 1.8593 2.3134 1.5656 2.1549
Actin 0.0346 0.0073 0.0155 0.0022 0.0055 0.0019 0.0228 0.0037 0.0126 0.0538 0.0142 0.0047 0.0223 0.0019 0.0128 0.0026 0.0003 0.0013
Bud 0.0007 0.0012 0.0005 0.0004 0.0123 0.0006 0.0005 0.0028 0.0003 0.0051 0.001 0.0014 0.0007 0.0013 0.0046 0.0023 0.0059 0.0022
Bud Neck 0.0042 0.0007 0.0058 0.0005 0.0005 0.009 0.0006 0.0004 0.0003 0.0026 0.0006 0.0011 0.003 0.0003 0.007 0.0005 0.001 0.0026
Bud Periphery 0.001 0.0009 0.0006 0.0004 0.0218 0.0014 0.0006 0.0019 0.0002 0.0059 0.0016 0.0015 0.0007 0.0006 0.0031 0.0042 0.003 0.0017
Bud Site 0.0163 0.0178 0.0046 0.001 0.0026 0.0003 0.0013 0.0072 0.0036 0.0211 0.0017 0.0003 0.003 0.0116 0.0085 0.0006 0.0016 0.0005
Cell Periphery 0.0011 0.0006 0.0004 0.0001 0.0003 0.0002 0.0003 0.0004 0.0002 0.0006 0.0002 0.0001 0.0006 0.0001 0.0004 0.0001 0.0003 0.0001
Cytoplasm 0.5472 0.8291 0.6976 0.794 0.7684 0.8349 0.7813 0.8953 0.9058 0.7182 0.637 0.8884 0.6343 0.8669 0.7419 0.7938 0.8208 0.8306
Cytoplasmic Foci 0.0283 0.0088 0.0147 0.0183 0.0055 0.0104 0.021 0.005 0.0085 0.0218 0.0553 0.0208 0.048 0.0192 0.0302 0.0239 0.0312 0.0215
Eisosomes 0.0004 0.0001 0.0002 0.0001 0 0.0001 0.0002 0 0.0002 0.0004 0.0001 0 0.0001 0 0.0003 0 0 0.0001
Endoplasmic Reticulum 0.0132 0.0023 0.0039 0.0047 0.0025 0.003 0.0117 0.0028 0.0052 0.0243 0.029 0.0059 0.0146 0.0027 0.0042 0.007 0.0042 0.007
Endosome 0.0304 0.0027 0.0125 0.0387 0.016 0.0073 0.0282 0.0031 0.0083 0.0459 0.0764 0.023 0.0487 0.0055 0.0158 0.0435 0.0224 0.0265
Golgi 0.0109 0.0011 0.006 0.0065 0.001 0.0019 0.0113 0.0004 0.0013 0.0148 0.0354 0.0042 0.013 0.0009 0.006 0.0041 0.0011 0.0044
Lipid Particles 0.0114 0.0004 0.0046 0.0142 0.0002 0.0014 0.0014 0.0001 0.0006 0.0059 0.0164 0.0006 0.0082 0.0003 0.0045 0.0003 0.0074 0.0024
Mitochondria 0.0144 0.0003 0.0076 0.0359 0.0009 0.0049 0.006 0.0001 0.0009 0.0386 0.0523 0.0164 0.0164 0.0004 0.0118 0.001 0.0008 0.0098
None 0.2522 0.1206 0.2113 0.0646 0.1291 0.1107 0.0836 0.0747 0.047 0.0127 0.0566 0.022 0.1462 0.0817 0.1294 0.0972 0.0843 0.083
Nuclear Periphery 0.0091 0.0005 0.0018 0.002 0.001 0.0042 0.0078 0.0004 0.001 0.0046 0.0038 0.0024 0.0036 0.0004 0.0011 0.0018 0.0012 0.0011
Nucleolus 0.0012 0.0001 0.0002 0.0005 0.0001 0.0001 0.0004 0 0.0001 0.0004 0.0002 0.0001 0.0006 0.0001 0.0004 0.0001 0.002 0.0001
Nucleus 0.0051 0.0016 0.0013 0.0019 0.0008 0.001 0.0083 0.0006 0.0012 0.0027 0.0016 0.001 0.0093 0.0013 0.0029 0.002 0.0036 0.0012
Peroxisomes 0.0113 0.0017 0.0042 0.0068 0.0001 0.0043 0.0032 0 0.0004 0.0095 0.0097 0.0007 0.0163 0.0026 0.0085 0.0029 0.0009 0.0009
Punctate Nuclear 0.003 0.0009 0.0031 0.0005 0.0002 0.001 0.0016 0.0001 0.0006 0.0019 0.0011 0.0003 0.0055 0.0005 0.0035 0.0006 0.0031 0.0006
Vacuole 0.0031 0.001 0.0026 0.005 0.0194 0.0011 0.0056 0.001 0.0014 0.0075 0.0038 0.0034 0.0042 0.0014 0.0026 0.0103 0.0046 0.0017
Vacuole Periphery 0.0009 0.0001 0.0009 0.0015 0.0116 0.0005 0.0024 0.0001 0.0003 0.0018 0.0022 0.0016 0.0007 0.0002 0.0005 0.0013 0.0006 0.0009

Sequencing Data

R1 R2
G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Post-START S/G2 Metaphase Anaphase Telophase
Gene Expression 6.9018 13.1358 5.0213 9.9211 6.6392 5.3404 14.0865 16.4343 10.8473 10.6293
Translational Efficiency 0.5253 0.9081 1.4691 0.5197 0.6781 0.5971 0.7801 0.5286 0.6016 0.5748

Hit Data

Dataset Hit
Protein Concentration ✔
Protein Localization ✘
Gene Expression ✘
Translational Efficiency ✘

Micrographs

Cell Count

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
811 1632 1393 1095 1177 1996 1436 2484 1988 3628 2829 3579

Protein Abundance

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
Mean 737.24 827.40 1079.92 1056.65 667.82 802.61 911.06 1149.55 696.14 813.76 994.21 1121.13
Standard Deviation 83.10 114.06 136.11 133.76 115.93 100.58 127.51 163.27 109.26 107.56 156.53 160.65
Intensity Change Log 2 — 0.166451 0.550718 0.519291 — 0.265240 0.448087 0.783538 — 0.214249 0.502847 0.650932

Localization Score (DeepLoc)

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
Actin 0.000184 0.000200 0.000360 0.001057 0.000171 0.000451 0.000069 0.001010 0.000177 0.000338 0.000212 0.001024
Bud Neck 0.015321 0.007498 0.003538 0.003383 0.005903 0.016997 0.003127 0.004405 0.009745 0.012724 0.003329 0.004092
Bud Site 0.008732 0.013911 0.013340 0.022623 0.008049 0.025195 0.005689 0.036453 0.008328 0.020119 0.009457 0.032222
Cell Periphery 0.000150 0.000060 0.000151 0.000123 0.000156 0.000090 0.000166 0.000069 0.000153 0.000077 0.000159 0.000085
Cytoplasm 0.534634* 0.630721* 0.606242* 0.669565* 0.568791* 0.538071* 0.532828* 0.769257* 0.554857* 0.579748* 0.568977* 0.738756*
Cytoplasmic Foci 0.264804* 0.163003 0.020963 0.067359 0.205308* 0.232341* 0.054153 0.034325 0.229579* 0.201150* 0.037810 0.044432
Eisosomes 0.000164 0.000033 0.000030 0.000020 0.000085 0.000088 0.000086 0.000012 0.000117 0.000063 0.000059 0.000014
Endoplasmic Reticulum 0.001640 0.001122 0.003271 0.000762 0.001812 0.001044 0.001083 0.000227 0.001741 0.001079 0.002161 0.000391
Endosome 0.021947 0.017439 0.004325 0.010695 0.014603 0.023222 0.005915 0.004871 0.017599 0.020621 0.005132 0.006653
Golgi 0.004334 0.003472 0.000149 0.003619 0.003576 0.009557 0.000154 0.007704 0.003886 0.006820 0.000152 0.006454
Lipid Particles 0.006137 0.001851 0.001914 0.001245 0.007311 0.003146 0.002204 0.000725 0.006832 0.002564 0.002061 0.000884
Mitochondria 0.004397 0.002141 0.000483 0.001398 0.003783 0.005147 0.000263 0.002684 0.004034 0.003795 0.000371 0.002291
Mitotic Spindle 0.001483 0.007606 0.016327 0.031312 0.001372 0.004699 0.004936 0.044542 0.001417 0.006007 0.010545 0.040495
None 0.001563 0.001134 0.004087 0.001693 0.018078 0.000633 0.006773 0.002082 0.011341 0.000858 0.005450 0.001963
Nuclear Periphery 0.000158 0.000230 0.001763 0.000303 0.000538 0.000149 0.000701 0.000133 0.000383 0.000185 0.001224 0.000185
Nuclear Periphery Foci 0.000380 0.000579 0.004634 0.003311 0.001084 0.000293 0.001024 0.000707 0.000797 0.000422 0.002801 0.001504
Nucleolus 0.000331 0.000511 0.000292 0.000150 0.001747 0.000450 0.000492 0.000187 0.001170 0.000477 0.000393 0.000176
Nucleus 0.039831 0.037880 0.201447* 0.025615 0.088831 0.034026 0.163842* 0.028855 0.068841 0.035760 0.182359* 0.027864
Peroxisomes 0.002531 0.002513 0.000232 0.002688 0.002056 0.006338 0.000746 0.004063 0.002250 0.004618 0.000493 0.003642
Vacuole 0.089454 0.104694 0.116174 0.151048 0.063963 0.094948 0.215187* 0.054560 0.074362 0.099332 0.166433 0.084081
Vacuole Periphery 0.001824 0.003403 0.000279 0.002031 0.002783 0.003114 0.000562 0.003128 0.002392 0.003244 0.000423 0.002792

Localization Changes (T-score)

R1 R2 R1 & R2
UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14 UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14 UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14
Actin -0.49 -7.78 -2.49 -2.42 -0.97 -3.85 2.90 -4.63 -0.80 -5.94 -3.77 -4.47 -4.96 -2.70 -3.69
Bud Neck 5.31 8.17 8.34 5.17 0.83 -10.40 5.10 3.19 12.96 -2.33 -3.41 9.38 8.59 13.54 -1.16
Bud Site -3.70 -0.78 -5.95 -3.05 -4.31 -9.46 1.59 -6.41 2.93 -8.00 -9.90 0.37 -8.48 0.46 -8.19
Cell Periphery 11.55 0.00 4.74 -4.78 4.54 5.11 1.28 9.51 4.04 10.01 9.47 1.03 11.44 1.28 11.88
Cytoplasm -8.12 4.43 -3.34 4.45 -8.14 2.85 5.13 -9.58 -14.28 -15.88 -3.22 7.10 -10.53 -8.33 -19.16
Cytoplasmic Foci 12.69 35.89 28.45 21.19 -14.70 -3.54 26.59 29.37 40.67 5.73 5.42 43.58 41.06 45.54 -5.28
Eisosomes 13.77 13.65 15.23 5.74 7.37 -0.35 2.02 18.49 14.67 15.22 10.04 12.43 22.43 16.02 17.56
Endoplasmic Reticulum 1.87 -7.37 4.40 3.58 15.18 2.46 3.02 6.59 4.19 8.63 3.11 -3.73 8.12 5.89 17.73
Endosome 2.41 10.63 8.93 8.93 -2.30 -5.65 8.89 11.94 17.61 4.80 -2.30 14.05 14.90 20.03 2.54
Golgi 1.74 9.46 4.49 3.40 -2.11 -7.48 8.48 1.50 7.32 -3.19 -5.65 12.46 3.14 7.22 -3.69
Lipid Particles 6.25 6.05 6.15 -0.25 0.68 6.12 8.31 9.55 5.77 2.66 8.75 10.34 11.46 5.34 3.28
Mitochondria 1.55 2.62 2.67 3.42 0.49 -1.11 3.72 2.86 5.27 -2.32 0.30 4.43 3.83 5.96 -1.75
Mitotic Spindle -3.30 -7.79 -7.93 -5.99 -2.75 -3.12 -4.31 -13.50 -11.99 -9.38 -4.54 -8.81 -15.61 -13.10 -8.87
None 0.72 -3.53 -0.30 -2.73 8.00 6.10 4.03 5.46 -14.52 2.77 6.09 3.61 5.28 -11.41 4.21
Nuclear Periphery -1.82 -27.67 -9.51 -5.37 23.06 4.38 -2.29 -0.77 -12.80 2.56 3.49 -18.30 -3.50 -12.55 19.39
Nuclear Periphery Foci -2.37 -15.74 -9.63 -8.94 2.12 6.27 -0.95 -1.45 -7.86 -0.52 4.46 -12.73 -8.05 -11.40 4.18
Nucleolus -1.65 0.19 1.56 2.42 1.71 4.21 4.37 4.55 0.44 0.85 3.74 4.42 4.59 1.56 0.86
Nucleus 0.41 -33.62 1.99 1.59 35.03 12.31 -8.67 8.61 -8.51 19.56 11.30 -27.21 7.98 -6.47 37.42
Peroxisomes 0.18 10.82 2.09 2.05 -5.08 -7.27 4.33 -0.37 7.10 -4.92 -6.65 8.83 0.68 6.98 -7.33
Vacuole -3.44 -12.67 -18.61 -17.19 -7.42 -7.83 -25.82 -20.65 -14.74 10.75 -8.13 -27.90 -26.61 -21.40 4.08
Vacuole Periphery -3.17 9.14 3.26 4.95 -1.58 -0.54 4.59 3.10 4.37 -1.31 -2.02 6.74 3.89 6.16 -1.97
ORF
Human Ortholog
Description Protein of unknown function; may interact with ribosomes, based on co-purification experiments; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm; YLL032C is not an essential gene
Localization
Cell Percentages cytoplasm (88%)
Cell Cycle Regulation No
Subcompartmental Group cytoplasm-9

YLL032C

YLL032C


Nearest neighbours ( genes)

correlation threshold

0.6

1.0

0.80


Biological Process (Gene Ontology)
Molecular Function (Gene Ontology)
Cellular Component (Gene Ontology)

Endocytosis
Temp Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Vph1-GFP)
37℃
RT
Cell Cycle Omics

CYCLoPs (Yll032c-GFP)
Gene / Allele Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Sac6-tdTomato)
Gene Images
Gene Images
Images are not yet available
Images are not yet available