Standard name
Human Ortholog
Description Splicing factor associated with the spliceosome; contains a U-box, a motif found in a class of ubiquitin ligases, and a WD40 domain; relocalizes to the cytosol in response to hypoxia
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Micrographs

ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 – – – – – –
Cell Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cytoplasm 0 0 0 0 0 0 0 0 0 0 0 0 0.09 0.11 0.17 0.05 0 0.05 0 0 0 0 0 0
Endoplasmic Reticulum 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Endosome 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Golgi 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Mitochondria 0 0 0.11 0.14 0.09 0.27 0.3 0.24 0.42 0.35 0.36 0.38 0 0 0 0 0 0.05 0 0 0 0 0 0
Nucleus 0.9 0.94 0.9 0.9 0.87 0.85 0.77 0.82 0.76 0.73 0.85 0.79 0.9 0.82 0.75 0.91 0.86 0.9 0.85 0.9 0.85 0.84 0.71 0.66
Nuclear Periphery 0 0 0 0 0 0 0.05 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0.16 0.08 0.16 0.08 0.08 0.14 0.19 0.17 0.17 0.18 0.17 0.19 0 0.08 0.12 0.07 0.1 0.05 0.09 0 0.09 0.07 0.09 0.12
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Vac/Vac Membrane 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0.07 0.11 0.14
ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 0 0 0 0 0 0 0 1 0 0 0 0 0 0 0 0 0 0
Bud 0 1 0 0 0 0 0 2 0 3 1 0 0 0 0 0 0 0 0 2 0 0 1 1
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 1 1 2 3 3 7 6 5 1 2 1 0 0 0 – – – – – –
Cell Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cytoplasm 0 0 2 3 8 4 8 6 2 7 3 3 15 33 46 10 4 2 0 0 0 0 2 0
Endoplasmic Reticulum 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 6 2 1 0 0 0 0 0 1
Endosome 0 0 0 0 0 0 0 0 0 0 0 0 0 4 1 3 0 0 1 1 1 0 4 1
Golgi 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 1 2 0 0 2 0
Mitochondria 6 6 16 12 20 47 90 86 66 85 64 75 1 1 4 4 2 2 1 1 0 0 1 4
Nucleus 159 250 137 75 194 150 228 297 120 179 151 155 146 240 201 196 89 37 160 248 130 107 178 148
Nuclear Periphery 1 1 1 0 1 1 14 6 3 7 6 5 0 1 4 0 0 0 0 0 0 0 0 0
Nucleolus 28 20 25 7 17 25 57 60 27 45 30 37 5 22 31 14 10 2 16 9 14 8 23 27
Peroxisomes 0 2 0 0 0 0 0 0 0 0 0 0 0 6 3 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 0 0 0 0 0 1 6 4 0 1 0 0 0 0 0 3 1
Vac/Vac Membrane 1 6 2 0 4 4 3 1 0 3 0 4 0 1 1 2 3 0 4 10 6 8 28 31
Unique Cell Count 177 266 152 83 222 176 298 361 158 244 178 197 162 293 269 215 103 41 189 278 154 128 251 224
Labelled Cell Count 195 286 183 97 245 232 402 461 221 336 261 284 169 317 296 235 111 44 189 278 154 128 251 224


Nucleus

(Huh et al. Nature 2003 425:686)
Screen WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 AF100 AF140 AF180
Mean Cell GFP Intensity (1e-4) 8.2 8.9 6.3 6.5 6.4 5.7 5.0 5.3 5.0 4.8 4.6 4.9 9.6 9.5 9.0 11.7 12.2 13.7 9.0 9.1 9.8
Std Deviation (1e-4) 1.4 1.8 1.1 1.5 1.4 1.3 1.2 1.4 1.2 1.4 1.1 1.3 2.7 2.9 3.2 3.5 3.9 3.5 1.8 2.2 2.7
Intensity Change (Log2) – – – 0.04 0.04 -0.14 -0.33 -0.24 -0.33 -0.38 -0.44 -0.37 0.62 0.59 0.51 0.9 0.95 1.12 0.51 0.53 0.64


Localization RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3
Cortical Patches 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cell Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cytoplasm 0 0 0 0 0 0 0 0 0 3.1 3.7 4.9 0 0 0
Endoplasmic Reticulum 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Endosome 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Golgi 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Mitochondria 0.9 -0.5 3.7 4.7 3.4 6.2 0 0 0 -3.9 -5.3 -4.2 -3.6 -2.6 0
Nucleus 0.1 -0.8 -1.3 -3.5 -2.3 -3.3 -4.0 -1.4 -2.9 0 -2.3 -3.8 0.3 -0.9 0
Nuclear Periphery 0 0 0 2.3 0 0 0 0 0 0 0 0 0 0 0
Nucleolus -1.7 -2.6 -0.6 0.7 0 0.2 0 0 0 -4.0 -2.9 -1.4 -3.0 -1.5 -1.9
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Vacuole 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0

Images

Circle plot not available

Protein Concentration and Protein Localization Data

R1 R2 R3
G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase
Concentration – – – – – – – – – – – – – – – – – –
Actin 0.1129 0 0.0003 0 0.0001 0 0.0043 0 0.0101 0.0001 0 0.0001 0.2848 0.0001 0.0003 0 0 0.004
Bud 0.0003 0 0 0 0 0 0.0004 0 0.0002 0.0001 0 0.0003 0.0037 0 0 0 0.0001 0.0001
Bud Neck 0.0009 0 0 0 0.0002 0.0003 0.0003 0 0.0001 0.0001 0.0002 0.001 0.0103 0 0 0 0.0002 0.0008
Bud Periphery 0.0002 0 0.0001 0 0.0001 0.0001 0.0005 0 0.0003 0.0005 0.0001 0.0011 0.006 0 0 0 0.0001 0.0004
Bud Site 0.0023 0 0 0 0 0 0.0017 0 0.0016 0.0001 0.0001 0.0002 0.124 0 0 0 0.0001 0.0003
Cell Periphery 0.0001 0 0 0 0 0.0001 0.0002 0 0.0001 0.0001 0 0.0002 0.0022 0 0 0 0.0001 0.0001
Cytoplasm 0.0044 0.0001 0 0 0.0001 0 0.0018 0 0.0002 0 0.0001 0.0001 0.0331 0.0001 0.0111 0.0001 0 0.0001
Cytoplasmic Foci 0.0052 0 0.0001 0 0 0 0.0124 0 0.0017 0 0 0.0001 0.0162 0 0.0004 0 0 0.0003
Eisosomes 0.0003 0 0 0 0 0 0.0001 0 0.0002 0 0 0 0.0033 0 0 0 0 0.0003
Endoplasmic Reticulum 0.002 0 0.0001 0 0 0 0.0013 0 0.0005 0.0001 0 0.0001 0.0526 0 0.0016 0 0 0.0002
Endosome 0.0043 0 0.0002 0 0.0001 0 0.0046 0 0.0034 0.0001 0 0.0004 0.0795 0 0.0008 0 0 0.0022
Golgi 0.0047 0 0.0001 0 0 0 0.0018 0 0.0041 0 0 0.0001 0.0594 0 0 0 0 0.0022
Lipid Particles 0.0017 0 0.0025 0 0 0 0.0073 0 0.0028 0 0 0.0002 0.0267 0 0.0002 0 0 0.0008
Mitochondria 0.0011 0.0002 0.0081 0.0001 0.0006 0.0002 0.0046 0.0001 0.0207 0.0021 0.0003 0.0018 0.0241 0.0007 0.0003 0.0002 0.0006 0.0801
None 0.0018 0 0.0001 0 0.0001 0 0.0044 0 0.0002 0 0 0.0001 0.008 0 0.0265 0 0 0.0002
Nuclear Periphery 0.004 0.0028 0.0056 0.0005 0.0075 0.0003 0.011 0.0016 0.0059 0.0031 0.0042 0.0022 0.0771 0.0103 0.005 0.0014 0.0001 0.0031
Nucleolus 0.0308 0.0255 0.0094 0.0062 0.1198 0.0635 0.0163 0.0157 0.02 0.0151 0.1443 0.0661 0.0015 0.0547 0.0106 0.0031 0.1814 0.0671
Nucleus 0.7976 0.9694 0.9711 0.9931 0.8661 0.9351 0.9079 0.9824 0.9225 0.9762 0.8471 0.9222 0.0591 0.9329 0.9414 0.9951 0.8168 0.8339
Peroxisomes 0.0019 0 0.0001 0 0 0 0.0069 0 0.0017 0 0 0 0.0091 0 0 0 0 0.0006
Punctate Nuclear 0.0227 0.0017 0.0003 0.0001 0.0047 0.0002 0.0101 0.0001 0.0027 0.0001 0.0033 0.0004 0.1059 0.0007 0.0011 0.0001 0.0001 0.0025
Vacuole 0.0004 0 0.0002 0 0.0001 0.0002 0.0013 0 0.0004 0.0007 0.0001 0.0018 0.0045 0 0.0002 0 0.0002 0.0003
Vacuole Periphery 0.0003 0.0001 0.0014 0 0.0003 0.0001 0.0009 0 0.0004 0.0013 0.0001 0.0013 0.009 0.0003 0.0002 0 0.0001 0.0005

Sequencing Data

R1 R2
G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Post-START S/G2 Metaphase Anaphase Telophase
Gene Expression 13.8655 20.8757 22.2678 19.2427 22.7081 14.5872 19.2935 19.1431 16.8479 19.6706
Translational Efficiency 1.5855 1.1193 0.8536 0.9999 0.9261 1.1616 1.0249 1.0869 1.1227 1.0183

Hit Data

Dataset Hit
Protein Concentration –
Protein Localization ✘
Gene Expression ✘
Translational Efficiency ✘

Micrographs

Cell Count

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
176 765 380 954 1121 1203 162 2086 1297 1968 542 3040

Protein Abundance

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
Mean 837.87 1038.41 1291.51 1190.20 957.27 1011.82 1386.82 1586.73 941.07 1022.16 1320.00 1462.29
Standard Deviation 110.34 202.49 172.04 229.30 149.52 180.94 177.32 341.44 150.49 190.05 179.03 361.05
Intensity Change Log 2 — 0.309578 0.624260 0.506406 — 0.079955 0.534783 0.729059 — 0.191689 0.577237 0.629394

Localization Score (DeepLoc)

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
Actin 0.000818 0.000763 0.001398 0.001529 0.000442 0.000744 0.001294 0.000555 0.000493 0.000751 0.001367 0.000860
Bud Neck 0.002080 0.008009 0.012809 0.032939 0.004569 0.008572 0.014875 0.028076 0.004232 0.008354 0.013427 0.029602
Bud Site 0.000338 0.000751 0.001017 0.004949 0.000396 0.000866 0.001036 0.001668 0.000388 0.000821 0.001023 0.002698
Cell Periphery 0.000036 0.000041 0.000037 0.000174 0.000071 0.000072 0.000040 0.000143 0.000066 0.000060 0.000038 0.000153
Cytoplasm 0.000261 0.000210 0.000144 0.005529 0.000470 0.000382 0.000144 0.001458 0.000442 0.000315 0.000144 0.002736
Cytoplasmic Foci 0.000511 0.000159 0.000050 0.001631 0.000036 0.000777 0.000037 0.000024 0.000101 0.000537 0.000046 0.000529
Eisosomes 0.000031 0.000036 0.000056 0.000040 0.000045 0.000037 0.000056 0.000027 0.000043 0.000036 0.000056 0.000031
Endoplasmic Reticulum 0.002484 0.002675 0.002491 0.002874 0.003137 0.003827 0.001827 0.002568 0.003049 0.003379 0.002292 0.002664
Endosome 0.000415 0.000245 0.000080 0.004345 0.000103 0.000420 0.000062 0.000382 0.000145 0.000352 0.000074 0.001626
Golgi 0.000422 0.000182 0.000151 0.004549 0.000059 0.000779 0.000120 0.000075 0.000108 0.000547 0.000142 0.001479
Lipid Particles 0.000781 0.000382 0.000069 0.000328 0.000033 0.000513 0.000043 0.000114 0.000134 0.000462 0.000061 0.000181
Mitochondria 0.001404 0.003552 0.001897 0.004589 0.000621 0.003273 0.002288 0.003229 0.000727 0.003381 0.002014 0.003656
Mitotic Spindle 0.001638 0.002344 0.000392 0.024917 0.001023 0.002774 0.000261 0.003850 0.001107 0.002607 0.000353 0.010461
None 0.002494 0.001202 0.000917 0.005530 0.001830 0.001114 0.001142 0.001682 0.001920 0.001148 0.000985 0.002889
Nuclear Periphery 0.000267 0.000547 0.000204 0.003311 0.000145 0.000511 0.000232 0.001720 0.000161 0.000525 0.000212 0.002219
Nuclear Periphery Foci 0.001148 0.001627 0.000138 0.001496 0.000259 0.000478 0.000058 0.000395 0.000380 0.000925 0.000114 0.000741
Nucleolus 0.151077 0.072852 0.100084 0.036560 0.067526 0.056075 0.069850 0.015789 0.078864 0.062597 0.091047 0.022307
Nucleus 0.829321* 0.902366* 0.874076* 0.852331* 0.918132* 0.916203* 0.903257* 0.935917* 0.906081* 0.910824* 0.882798* 0.909687*
Peroxisomes 0.003985 0.001195 0.002914 0.001757 0.000613 0.000831 0.002572 0.000101 0.001071 0.000972 0.002812 0.000621
Vacuole 0.000322 0.000454 0.000847 0.006468 0.000281 0.000915 0.000595 0.001364 0.000287 0.000736 0.000772 0.002966
Vacuole Periphery 0.000168 0.000409 0.000229 0.004152 0.000208 0.000837 0.000209 0.000864 0.000202 0.000670 0.000223 0.001896

Localization Changes (T-score)

R1 R2 R1 & R2
UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14 UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14 UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14
Actin 0.60 -2.04 -2.60 -6.43 -1.43 -4.62 -10.23 -2.77 1.65 7.51 -3.95 -13.51 -6.15 -3.03 6.26
Bud Neck -5.41 -10.37 -14.53 -12.09 -9.12 -4.93 -8.21 -18.36 -14.61 -7.77 -6.47 -12.78 -23.06 -19.01 -12.64
Bud Site -1.22 -4.70 -8.20 -7.54 -6.62 -2.32 -5.51 -6.06 -3.25 -2.23 -2.77 -8.05 -10.18 -7.55 -5.97
Cell Periphery 0.08 -0.06 -4.50 -5.03 -5.05 -0.02 1.01 -1.46 -1.59 -2.39 0.37 1.23 -2.32 -3.08 -3.95
Cytoplasm 1.07 1.14 -3.24 -4.10 -4.13 0.49 0.95 -2.43 -5.17 -6.14 0.85 1.26 -4.03 -5.87 -6.25
Cytoplasmic Foci 1.24 1.48 -0.61 -2.68 -3.05 -1.90 -0.36 0.83 1.94 1.52 -1.67 1.17 -1.80 0.78 -2.81
Eisosomes -0.88 -5.05 -2.44 -2.93 4.40 2.49 -2.81 6.07 5.51 8.51 2.37 -4.32 4.30 3.36 10.56
Endoplasmic Reticulum 0.06 0.13 -0.77 -1.64 -1.70 -2.67 6.11 3.00 5.04 -3.23 -1.46 3.18 1.54 3.09 -1.75
Endosome 0.72 1.25 -2.70 -3.18 -3.40 -2.83 1.63 -2.47 0.60 -2.89 -2.37 1.42 -3.67 -2.75 -3.98
Golgi 0.93 0.94 -2.05 -2.36 -2.36 -1.81 -4.77 -1.91 1.74 2.62 -1.77 -1.57 -2.37 -0.84 -2.20
Lipid Particles 0.86 1.47 1.05 0.29 -2.57 -2.08 -1.17 -1.01 1.68 -0.80 -1.71 1.01 -0.23 1.66 -1.71
Mitochondria -1.59 -1.29 -5.54 -0.92 -6.92 -2.94 -10.29 -6.09 -0.21 -1.96 -3.58 -11.96 -8.99 -0.62 -4.85
Mitotic Spindle 0.60 1.19 -4.88 -6.05 -6.54 -1.31 1.11 -2.99 -1.69 -4.45 -1.05 0.95 -6.39 -5.88 -7.54
None 2.13 2.36 -1.07 -3.85 -4.13 1.41 1.12 -0.57 -2.60 -2.24 1.97 2.19 -1.63 -4.58 -4.97
Nuclear Periphery -1.47 0.41 -3.67 -3.20 -3.72 -2.95 -3.15 -5.46 -4.21 -5.07 -3.50 -3.31 -6.56 -5.24 -6.28
Nuclear Periphery Foci -0.06 1.82 0.51 0.59 -3.78 -0.84 2.05 -0.52 0.40 -2.28 -1.31 2.24 -0.87 0.87 -3.81
Nucleolus 6.43 3.56 9.68 7.30 8.35 2.73 -0.12 13.81 12.99 5.84 4.01 -2.06 15.25 14.90 11.50
Nucleus -5.32 -2.79 -1.55 5.80 1.80 0.35 1.65 -1.67 -1.97 -2.53 -1.06 3.86 0.76 1.99 -3.46
Peroxisomes 1.02 0.40 0.93 -0.38 2.57 -1.11 -4.61 4.64 4.38 6.08 0.25 -3.83 1.33 2.11 8.24
Vacuole -0.04 -1.86 -4.62 -4.71 -4.24 -2.23 -4.04 -3.48 -0.96 -1.99 -2.30 -5.05 -5.74 -4.63 -4.28
Vacuole Periphery -2.66 -1.88 -2.92 -2.61 -2.84 -2.73 -0.17 -2.39 0.34 -2.38 -3.22 -1.20 -3.72 -1.86 -3.62
Standard name
Human Ortholog
Description Splicing factor associated with the spliceosome; contains a U-box, a motif found in a class of ubiquitin ligases, and a WD40 domain; relocalizes to the cytosol in response to hypoxia
Localization
Cell Percentages nucleus (100%)
Cell Cycle Regulation No
Subcompartmental Group nucleus-4

Prp19

Prp19


Nearest neighbours ( genes)

correlation threshold

0.6

1.0

0.80


Biological Process (Gene Ontology)
Molecular Function (Gene Ontology)
Cellular Component (Gene Ontology)

Endocytosis
Temp Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Vph1-GFP)
37℃
RT
Cell Cycle Omics

CYCLoPs (Prp19-GFP)
Gene / Allele Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Sac6-tdTomato)
Gene Images
Gene Images
Images are not yet available
Images are not yet available