Standard name
Human Ortholog
Description Protein of unknown function; contains an N-terminal epsin-like domain; proposed to be involved in the trafficking of Arn1p in the absence of ferrichrome
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Micrographs

ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud 0 0.11 0 0 0 0 0 0 0 0 0 0.05 0 0 0 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0.05 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 – – – – – –
Cell Periphery 0.31 0.35 0.34 0.29 0.19 0.14 0.12 0.07 0.06 0.06 0 0.05 0.47 0.4 0.42 0.23 0.28 0.23 0.05 0 0.23 0.07 0 0
Cytoplasm 0.87 0.88 0.86 0.85 0.95 0.97 0.96 0.96 0.89 0.92 0.85 0.89 0.83 0.9 0.91 0.94 0.88 0.86 0.77 0.86 0.55 0.79 0.85 0.86
Endoplasmic Reticulum 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0.05 0 0.06 0.09 0 0.08 0 0 0
Endosome 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Golgi 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Mitochondria 0 0 0 0 0 0 0 0 0.1 0.1 0.16 0.19 0 0 0 0 0 0 0 0 0 0 0 0
Nucleus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nuclear Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Vac/Vac Membrane 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 3 2 2 5 4 3 4 5 2 1 1 2 2 2 1 1 0 2 2 2 3 4 5 4
Bud 4 19 3 5 5 2 4 5 8 4 3 7 4 6 3 1 2 2 0 0 0 0 0 1
Bud Neck 0 0 0 0 0 0 0 1 1 0 0 0 1 0 1 0 0 0 4 8 4 14 12 7
Bud Site 0 0 0 0 0 0 0 0 0 1 0 0 0 0 0 0 0 0 – – – – – –
Cell Periphery 42 59 28 36 23 33 26 18 11 16 4 6 104 112 154 42 41 40 7 8 24 19 14 10
Cytoplasm 118 150 71 104 115 230 213 230 161 241 77 115 186 253 336 169 128 148 112 173 56 220 307 283
Endoplasmic Reticulum 4 4 2 5 0 2 3 2 2 0 2 4 8 6 9 9 3 10 12 2 8 8 5 5
Endosome 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 2 1 0 1 0 0 2 1 4
Golgi 1 0 0 0 0 0 0 0 0 1 0 0 0 0 1 0 0 0 0 0 0 1 0 1
Mitochondria 1 2 0 0 1 2 4 1 18 25 15 24 0 0 1 3 3 6 2 2 1 1 3 1
Nucleus 1 1 1 2 0 0 1 3 1 1 3 0 0 1 1 2 3 6 0 0 0 0 2 0
Nuclear Periphery 0 0 0 0 0 0 0 0 0 0 1 0 0 0 0 0 0 0 0 0 0 1 0 0
Nucleolus 0 0 1 0 0 0 0 0 0 0 0 0 0 0 1 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 1 0 0 0 1 0 0 0 0 0 1 0 2 0 2 0 0 0 0 0 1
Vac/Vac Membrane 0 0 0 0 0 0 0 1 2 0 1 0 0 0 1 1 2 1 0 2 0 0 0 2
Unique Cell Count 135 171 83 123 121 238 223 240 181 263 91 129 223 282 368 179 146 173 146 203 103 278 361 330
Labelled Cell Count 174 237 108 158 148 272 255 267 206 290 107 158 305 381 509 232 183 217 146 203 103 278 361 330


Ambiguous

(Huh et al. Nature 2003 425:686)
Screen WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 AF100 AF140 AF180
Mean Cell GFP Intensity (1e-4) 8.8 6.5 7.8 8.5 7.8 6.6 6.1 6.3 5.3 5.6 5.2 5.2 8.5 8.2 8.1 7.5 9.0 10.2 9.7 9.5 9.3
Std Deviation (1e-4) 1.2 0.9 1.4 1.5 1.7 1.2 0.9 1.2 1.2 0.8 1.0 0.6 1.1 1.0 1.1 1.5 1.6 1.9 1.4 1.3 1.4
Intensity Change (Log2) – – – 0.12 0.0 -0.23 -0.35 -0.29 -0.56 -0.46 -0.59 -0.59 0.13 0.08 0.06 -0.05 0.21 0.39 0.32 0.29 0.26


Localization RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3
Cortical Patches 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cell Periphery -0.7 -2.4 -4.0 -4.5 -5.9 -5.9 -6.6 -5.0 -5.6 2.0 1.0 1.4 -1.7 -0.9 -1.8
Cytoplasm -0.2 2.3 3.6 3.0 3.2 0.8 1.6 -0.2 0.8 -0.5 1.1 1.6 2.4 0.5 0
Endoplasmic Reticulum 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Endosome 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Golgi 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Mitochondria 0 0 0 0 0 3.0 2.9 3.9 4.2 0 0 0 0 0 0
Nucleus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nuclear Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Vacuole 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0

Images

Circle plot not available

Protein Concentration and Protein Localization Data

R1 R2 R3
G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase
Concentration 4.3578 5.2209 4.5007 3.7632 4.1393 4.7268 6.9754 8.3091 7.6637 6.7503 7.6395 7.7275 4.7135 5.6624 5.0512 4.9483 4.6064 4.8888
Actin 0.0092 0.002 0.0056 0.0105 0.0058 0.003 0.0457 0.0183 0.0067 0.0028 0.0027 0.0071 0.0119 0.0019 0.0044 0.0027 0.004 0.0017
Bud 0.0005 0.0007 0.0011 0.0004 0.0005 0.0025 0.0005 0.0006 0.0018 0.0003 0.0002 0.0004 0.0002 0.0002 0.0003 0.0003 0.0001 0.0001
Bud Neck 0.0035 0.0031 0.0083 0.0038 0.0229 0.0345 0.0015 0.0048 0.0046 0.004 0.0031 0.0468 0.0019 0.0012 0.0047 0.0029 0.0028 0.0348
Bud Periphery 0.0015 0.0018 0.0048 0.0025 0.0014 0.002 0.0008 0.0019 0.0026 0.0012 0.0009 0.0025 0.0006 0.0006 0.0013 0.0015 0.0004 0.0006
Bud Site 0.0185 0.0238 0.0191 0.0018 0.0048 0.0006 0.0055 0.0429 0.024 0.0026 0.0007 0.0008 0.0027 0.019 0.006 0.0053 0.0004 0.0004
Cell Periphery 0.0515 0.0157 0.0436 0.0382 0.031 0.0142 0.0685 0.0475 0.0455 0.0482 0.0652 0.0245 0.071 0.0429 0.0575 0.0811 0.0294 0.0227
Cytoplasm 0.8766 0.9372 0.8949 0.8993 0.9115 0.927 0.7244 0.8568 0.8529 0.8622 0.8562 0.8534 0.8426 0.9086 0.8563 0.8005 0.8663 0.92
Cytoplasmic Foci 0.002 0.0011 0.0023 0.0026 0.0027 0.0012 0.0196 0.007 0.0075 0.026 0.0151 0.0074 0.0045 0.0015 0.0055 0.001 0.0047 0.0022
Eisosomes 0.0006 0.0001 0.0001 0.0002 0.0001 0.0001 0.0008 0.0003 0.0001 0 0 0.0005 0.0004 0.0001 0.0001 0.0002 0.0001 0
Endoplasmic Reticulum 0.0071 0.0034 0.0086 0.0036 0.0032 0.0016 0.008 0.0033 0.0085 0.005 0.0062 0.0071 0.0129 0.0039 0.0056 0.0426 0.0053 0.0024
Endosome 0.0012 0.0003 0.0009 0.0235 0.0011 0.0003 0.0255 0.0011 0.0068 0.0076 0.0204 0.0099 0.0074 0.0002 0.0117 0.0019 0.0187 0.0053
Golgi 0.0009 0.0002 0.0006 0.0041 0.0004 0.0002 0.0237 0.0021 0.0084 0.0044 0.0214 0.0063 0.0049 0.0002 0.0043 0.0008 0.0015 0.0027
Lipid Particles 0.0013 0.0004 0.0017 0.0008 0.001 0.0001 0.0087 0.0023 0.0014 0.0166 0.0026 0.0082 0.0047 0.0007 0.0073 0.0086 0.0037 0.0013
Mitochondria 0.0004 0 0.0001 0.002 0 0.0001 0.0139 0.0004 0.0116 0.0004 0.001 0.0103 0.005 0 0.003 0.0003 0.0013 0.001
None 0.0203 0.0088 0.0045 0.0028 0.0096 0.0092 0.0375 0.007 0.0113 0.0008 0.0008 0.0117 0.0189 0.0174 0.0211 0.0416 0.0096 0.0021
Nuclear Periphery 0.0006 0.0002 0.0004 0.0003 0.0003 0.0003 0.0013 0.0002 0.0006 0.0002 0.0002 0.0006 0.0022 0.0002 0.0047 0.0028 0.0169 0.0002
Nucleolus 0.0002 0 0.0001 0 0.0002 0.0001 0.0002 0.0002 0.0002 0.0004 0 0 0.0004 0 0.0002 0.0001 0.0006 0
Nucleus 0.0006 0.0005 0.0007 0.0003 0.0006 0.0019 0.0005 0.0004 0.0007 0.0003 0.0001 0.0003 0.0007 0.0003 0.0009 0.0008 0.0049 0.0002
Peroxisomes 0.0002 0 0 0.0001 0 0 0.0093 0.0007 0.0012 0.0123 0.0016 0.0006 0.0046 0 0.0014 0 0.0009 0.0013
Punctate Nuclear 0.0003 0.0001 0.0004 0 0.0006 0.0003 0.001 0.0001 0.0001 0.0021 0.0001 0.0002 0.0006 0.0001 0.0007 0.0002 0.0266 0.0002
Vacuole 0.0028 0.0007 0.0021 0.002 0.0022 0.0006 0.0024 0.002 0.0033 0.0023 0.0013 0.0012 0.0016 0.0011 0.0023 0.0047 0.0013 0.0007
Vacuole Periphery 0.0001 0 0.0001 0.0011 0 0 0.0006 0 0.0002 0.0001 0.0001 0.0003 0.0003 0 0.0006 0.0002 0.0006 0.0001

Sequencing Data

R1 R2
G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Post-START S/G2 Metaphase Anaphase Telophase
Gene Expression 13.3476 10.6062 13.7729 12.1915 16.3444 22.2337 15.4156 16.2853 18.6545 13.2464
Translational Efficiency 1.0373 1.4104 1.0651 1.4109 1.2068 0.7702 0.9284 1.1774 0.9236 0.9975

Hit Data

Dataset Hit
Protein Concentration ✘
Protein Localization ✘
Gene Expression ✘
Translational Efficiency ✘

Micrographs

Cell Count

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
1907 1253 1350 1679 1602 2047 2567 1846 3509 3300 3917 3525

Protein Abundance

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
Mean 915.24 1036.94 1081.79 1102.71 892.14 1070.95 1128.21 1099.42 904.69 1058.04 1112.21 1100.99
Standard Deviation 114.54 144.48 126.62 147.96 119.34 132.58 144.04 145.65 117.32 138.21 140.04 146.76
Intensity Change Log 2 — 0.180110 0.241198 0.268831 — 0.263549 0.338694 0.301401 — 0.221900 0.290147 0.285000

Localization Score (DeepLoc)

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
Actin 0.006342 0.009252 0.014433 0.029624 0.002954 0.006979 0.008798 0.018108 0.004795 0.007842 0.010740 0.023593
Bud Neck 0.041402 0.020548 0.031875 0.017731 0.016966 0.052354 0.026117 0.017970 0.030246 0.040278 0.028101 0.017856
Bud Site 0.089777 0.125234* 0.091283 0.128709* 0.079057 0.104877* 0.079349 0.121185* 0.084883 0.112606* 0.083462 0.124769*
Cell Periphery 0.012676 0.008851 0.005250 0.004630 0.002859 0.006188 0.003513 0.003516 0.008194 0.007199 0.004112 0.004046
Cytoplasm 0.699967* 0.654885* 0.587607* 0.531193* 0.691122* 0.614894* 0.606101* 0.539282* 0.695929* 0.630079* 0.599727* 0.535429*
Cytoplasmic Foci 0.063977 0.094260 0.052069 0.050407 0.122575 0.084382 0.043589 0.080154 0.090729 0.088133 0.046512 0.065985
Eisosomes 0.007007 0.001481 0.001930 0.001603 0.000968 0.003627 0.001267 0.000787 0.004250 0.002812 0.001495 0.001176
Endoplasmic Reticulum 0.002858 0.004336 0.004854 0.002560 0.004194 0.003050 0.002731 0.001925 0.003468 0.003538 0.003463 0.002227
Endosome 0.005120 0.007984 0.005403 0.005970 0.006220 0.010236 0.002905 0.012146 0.005622 0.009381 0.003766 0.009204
Golgi 0.013688 0.010192 0.001177 0.003376 0.009610 0.009618 0.000589 0.002544 0.011826 0.009836 0.000791 0.002941
Lipid Particles 0.005828 0.007070 0.008047 0.006191 0.003894 0.004378 0.005743 0.014297 0.004945 0.005400 0.006537 0.010436
Mitochondria 0.002122 0.000400 0.000279 0.001360 0.000510 0.001427 0.000202 0.000135 0.001386 0.001037 0.000229 0.000719
Mitotic Spindle 0.000156 0.000443 0.000382 0.008271 0.000182 0.001667 0.001263 0.002447 0.000168 0.001203 0.000959 0.005221
None 0.001528 0.000615 0.000709 0.000314 0.003165 0.000413 0.001352 0.000923 0.002276 0.000489 0.001130 0.000633
Nuclear Periphery 0.000056 0.000023 0.000080 0.000103 0.000050 0.000035 0.000096 0.000051 0.000054 0.000030 0.000090 0.000076
Nuclear Periphery Foci 0.000575 0.001698 0.002118 0.002351 0.001694 0.000734 0.003160 0.003126 0.001086 0.001100 0.002801 0.002757
Nucleolus 0.000177 0.000102 0.000132 0.000084 0.000180 0.000405 0.000145 0.000128 0.000178 0.000290 0.000141 0.000107
Nucleus 0.011286 0.007605 0.034264 0.023712 0.013726 0.011833 0.063020 0.011941 0.012400 0.010227 0.053109 0.017548
Peroxisomes 0.003552 0.003411 0.002269 0.007747 0.002037 0.004923 0.001577 0.006180 0.002860 0.004349 0.001815 0.006927
Vacuole 0.030676 0.040789 0.155643 0.172308 0.037136 0.077208 0.148351 0.162869 0.033625 0.063380 0.150864 0.167365
Vacuole Periphery 0.001231 0.000819 0.000195 0.001757 0.000899 0.000773 0.000133 0.000284 0.001080 0.000791 0.000155 0.000986

Localization Changes (T-score)

R1 R2 R1 & R2
UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14 UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14 UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14
Actin -3.77 -6.39 -10.94 -8.55 -5.53 -7.90 -9.26 -11.95 -7.85 -6.92 -6.88 -9.49 -15.65 -11.84 -9.46
Bud Neck 9.04 4.65 13.03 4.04 7.33 -14.23 -3.64 0.52 15.47 4.87 -5.26 3.52 11.27 15.81 8.70
Bud Site -6.73 1.21 -3.24 3.53 -3.93 -6.13 2.17 -6.84 -1.26 -9.37 -8.56 3.32 -7.03 1.33 -9.97
Cell Periphery 4.35 11.70 13.70 6.61 2.24 -10.01 -0.79 -0.74 8.29 -0.07 2.27 12.68 12.89 9.90 1.30
Cytoplasm 5.90 15.10 24.25 16.43 7.19 11.04 15.22 20.48 10.93 7.11 13.01 21.93 31.68 18.89 10.51
Cytoplasmic Foci -7.95 3.50 5.31 12.12 1.54 9.81 22.63 10.14 0.83 -11.84 0.95 18.97 9.84 8.71 -8.38
Eisosomes 15.60 14.19 15.09 1.16 2.19 -9.99 -1.12 2.98 11.35 4.44 5.73 14.23 15.50 9.85 3.13
Endoplasmic Reticulum -1.95 -2.77 1.67 2.92 3.91 1.38 2.11 3.51 2.35 3.24 -0.12 0.16 3.92 3.57 4.86
Endosome -3.58 -0.45 0.69 3.59 0.94 -5.11 6.66 -5.39 -1.29 -9.82 -6.83 5.67 -4.55 1.47 -8.16
Golgi 4.78 23.22 15.85 11.27 -2.58 -0.00 22.09 12.59 11.84 -3.94 4.10 31.74 20.33 16.13 -4.84
Lipid Particles -1.56 -2.26 0.33 1.82 2.47 -1.26 -2.88 -7.76 -7.48 -6.02 -1.11 -2.80 -6.51 -5.95 -4.09
Mitochondria 4.64 5.01 2.39 -1.63 -2.05 -2.63 8.55 9.95 3.75 4.25 1.20 5.79 3.41 1.96 -1.91
Mitotic Spindle -1.44 -2.21 -4.62 -4.29 -4.41 -2.34 -2.87 -3.17 -0.79 -1.43 -2.62 -3.22 -5.59 -3.64 -4.25
None 2.07 1.92 2.79 2.12 1.90 3.86 2.73 2.86 -1.04 0.87 4.44 3.09 3.73 -0.46 1.81
Nuclear Periphery 1.12 -1.22 -1.85 -5.49 -1.49 0.59 -2.86 -0.40 -3.34 8.54 1.16 -3.01 -1.62 -6.19 2.69
Nuclear Periphery Foci -6.83 -9.75 -6.55 -1.92 -0.30 6.77 -6.76 -6.34 -12.29 0.55 -0.11 -11.63 -9.69 -9.63 0.90
Nucleolus 2.33 1.47 2.89 2.17 4.03 -1.14 3.12 2.35 1.39 0.75 -0.90 2.16 3.44 1.48 2.66
Nucleus 5.77 -15.33 -12.81 -15.84 3.89 2.56 -29.27 1.26 -1.43 29.86 4.18 -32.99 -10.37 -12.98 25.35
Peroxisomes 0.66 7.34 -3.89 -4.05 -5.86 -7.12 4.18 -8.42 -1.48 -9.64 -5.57 9.71 -7.73 -3.54 -10.56
Vacuole -5.19 -28.46 -36.66 -33.94 -7.83 -16.11 -37.08 -32.56 -22.27 -1.87 -17.44 -47.58 -48.89 -38.30 -7.35
Vacuole Periphery 1.08 2.97 0.06 -0.89 -2.52 1.09 7.32 5.91 8.28 -3.32 1.40 4.74 1.36 0.41 -2.91
Standard name
Human Ortholog
Description Protein of unknown function; contains an N-terminal epsin-like domain; proposed to be involved in the trafficking of Arn1p in the absence of ferrichrome
Localization
Cell Percentages cytoplasm (93%)
Cell Cycle Regulation No
Subcompartmental Group cytoplasm-10

Ent4

Ent4


Nearest neighbours ( genes)

correlation threshold

0.6

1.0

0.80


Biological Process (Gene Ontology)
Molecular Function (Gene Ontology)
Cellular Component (Gene Ontology)

Endocytosis
Temp Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Vph1-GFP)
37℃
RT
Cell Cycle Omics

CYCLoPs (Ent4-GFP)
Gene / Allele Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Sac6-tdTomato)
Gene Images
Gene Images
Images are not yet available
Images are not yet available