Standard name | |
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Human Ortholog | |
Description | Ribosomal 60S subunit protein L8B; required for processing of 27SA3 pre-rRNA to 27SB pre-rRNA during assembly of large ribosomal subunit; depletion leads to a turnover of pre-rRNA; L8 binds to Domain I of 25S and 5.8 S rRNAs; mutation results in decreased amounts of free 60S subunits; homologous to mammalian ribosomal protein L7A, no bacterial homolog; RPL8B has a paralog, RPL8A, that arose from the whole genome duplication |
ensLOC | DeepLoc | |||||||||||||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Localization | WT1 | WT2 | WT3 | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 | WT1 | WT2 | WT3 | AF100 | AF140 | AF180 |
Cortical Patches | – | 0 | 0 | 0 | 0 | 0 | 3 | 4 | 2 | 5 | 5 | 8 | – | 0 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Bud | – | 0 | 2 | 0 | 0 | 1 | 1 | 2 | 2 | 1 | 2 | 1 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1 |
Bud Neck | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Bud Site | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 0 | – | 0 | 0 | 0 | 0 | 0 | – | – | – | – | – | – |
Cell Periphery | – | 0 | 3 | 1 | 5 | 8 | 17 | 22 | 8 | 19 | 25 | 23 | – | 0 | 4 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 0 |
Cytoplasm | – | 109 | 181 | 183 | 307 | 240 | 278 | 311 | 138 | 154 | 126 | 184 | – | 65 | 117 | 17 | 104 | 105 | 27 | 98 | 161 | 24 | 97 | 113 |
Endoplasmic Reticulum | – | 0 | 3 | 1 | 8 | 11 | 47 | 65 | 25 | 26 | 40 | 56 | – | 1 | 4 | 1 | 4 | 7 | 0 | 0 | 4 | 0 | 0 | 0 |
Endosome | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Golgi | – | 0 | 1 | 0 | 1 | 0 | 3 | 1 | 2 | 1 | 3 | 10 | – | 0 | 1 | 0 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Mitochondria | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 3 | 1 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Nucleus | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 0 | 1 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1 |
Nuclear Periphery | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Nucleolus | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1 |
Peroxisomes | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
SpindlePole | – | 0 | 0 | 0 | 0 | 0 | 1 | 0 | 0 | 0 | 2 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Vac/Vac Membrane | – | 0 | 0 | 0 | 0 | 1 | 0 | 0 | 0 | 0 | 2 | 1 | – | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 1 | 0 | 0 | 0 |
Unique Cell Count | 109 | 183 | 184 | 316 | 252 | 338 | 384 | 172 | 195 | 189 | 266 | 66 | 122 | 17 | 105 | 105 | 29 | 105 | 173 | 29 | 102 | 122 | ||
Labelled Cell Count | 109 | 190 | 185 | 321 | 261 | 350 | 405 | 177 | 208 | 209 | 286 | 66 | 127 | 18 | 109 | 112 | 29 | 105 | 173 | 29 | 102 | 122 |
Screen | WT1 | WT2 | WT3 | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 | AF100 | AF140 | AF180 |
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Mean Cell GFP Intensity (1e-4) | – | 143.3 | 149.0 | 154.1 | 124.6 | 89.1 | 74.4 | 67.0 | 59.6 | 61.2 | 54.0 | 57.9 | – | 143.2 | 127.5 | 134.7 | 180.4 | 209.4 | 157.5 | 154.7 | 146.5 |
Std Deviation (1e-4) | – | 39.0 | 31.0 | 32.6 | 22.8 | 20.0 | 15.3 | 14.7 | 13.8 | 12.5 | 13.6 | 13.2 | – | 36.1 | 42.3 | 18.9 | 45.6 | 56.4 | 49.4 | 60.9 | 60.4 |
Intensity Change (Log2) | – | – | – | 0.05 | -0.26 | -0.74 | -1.0 | -1.15 | -1.32 | -1.28 | -1.46 | -1.36 | – | -0.06 | -0.23 | -0.15 | 0.28 | 0.49 | 0.08 | 0.05 | -0.02 |
Localization | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 |
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Cortical Patches | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 |
Bud | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 |
Bud Neck | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 |
Bud Site | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 |
Cell Periphery | 0 | 0 | 0 | 1.9 | 2.2 | 1.6 | 3.4 | 4.2 | 3.1 | – | 0 | 0 | 0 | 0 | 0 |
Cytoplasm | 0.6 | -1.3 | -2.1 | -5.6 | -5.9 | -5.8 | -6.1 | -8.2 | -7.9 | – | -0.3 | -1.7 | 0.4 | 0.1 | 1.1 |
Endoplasmic Reticulum | 0 | 0 | 1.6 | 4.5 | 5.2 | 4.5 | 4.3 | 5.9 | 6.0 | – | 0 | 0 | 0 | 0 | 0 |
Endosome | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 |
Golgi | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 |
Mitochondria | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 |
Nucleus | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 |
Nuclear Periphery | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 |
Nucleolus | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 |
Peroxisomes | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 |
SpindlePole | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 |
Vacuole | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 |
R1 | R2 | R3 | ||||||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | |
Concentration | 146.2775 | 194.0582 | 167.7297 | 173.2947 | 148.1032 | 97.5011 | 143.8801 | 183.2698 | 171.2399 | 165.9379 | 128.7563 | 183.2385 | 149.9039 | 217.3154 | 191.9293 | 198.5161 | 173.3014 | 132.1751 |
Actin | 0.0077 | 0.0004 | 0.0084 | 0.0004 | 0.0081 | 0.0158 | 0.0049 | 0.0005 | 0.0009 | 0.0008 | 0.0011 | 0.0012 | 0.0218 | 0.0004 | 0.0004 | 0.0005 | 0.0274 | 0.0021 |
Bud | 0.0021 | 0.0013 | 0.0014 | 0.0022 | 0.0014 | 0.0099 | 0.0013 | 0.0008 | 0.0011 | 0.0033 | 0.003 | 0.0006 | 0.0012 | 0.0008 | 0.0014 | 0.0024 | 0.0016 | 0.0008 |
Bud Neck | 0.0007 | 0.0011 | 0.0015 | 0.0007 | 0.0008 | 0.0097 | 0.0007 | 0.0007 | 0.0006 | 0.0018 | 0.0014 | 0.0009 | 0.0021 | 0.0005 | 0.0008 | 0.001 | 0.0018 | 0.0006 |
Bud Periphery | 0.0021 | 0.0008 | 0.0018 | 0.0008 | 0.0009 | 0.0069 | 0.0013 | 0.0005 | 0.0007 | 0.002 | 0.004 | 0.0008 | 0.0012 | 0.0003 | 0.0005 | 0.0008 | 0.0005 | 0.0004 |
Bud Site | 0.0008 | 0.0006 | 0.0013 | 0.0003 | 0.0009 | 0.0042 | 0.0011 | 0.0007 | 0.0005 | 0.0004 | 0.0012 | 0.0001 | 0.0053 | 0.0005 | 0.0003 | 0.0003 | 0.0005 | 0.0004 |
Cell Periphery | 0.0243 | 0.0095 | 0.0257 | 0.0059 | 0.0087 | 0.026 | 0.0258 | 0.0074 | 0.0189 | 0.0087 | 0.0155 | 0.001 | 0.0095 | 0.0071 | 0.0026 | 0.0212 | 0.0117 | 0.0018 |
Cytoplasm | 0.6204 | 0.8833 | 0.7069 | 0.6492 | 0.7401 | 0.3984 | 0.6976 | 0.9296 | 0.808 | 0.6773 | 0.6597 | 0.9345 | 0.7347 | 0.9453 | 0.8445 | 0.7454 | 0.7303 | 0.6838 |
Cytoplasmic Foci | 0.0093 | 0.0012 | 0.0134 | 0.0015 | 0.0056 | 0.0377 | 0.0052 | 0.0009 | 0.0078 | 0.0015 | 0.0193 | 0.0034 | 0.0078 | 0.0006 | 0.0061 | 0.0014 | 0.0065 | 0.0696 |
Eisosomes | 0.0006 | 0 | 0.0003 | 0 | 0.0003 | 0.0024 | 0.0003 | 0 | 0.0002 | 0 | 0.0005 | 0 | 0.0011 | 0 | 0.0001 | 0 | 0.0003 | 0.0015 |
Endoplasmic Reticulum | 0.0704 | 0.0366 | 0.016 | 0.0157 | 0.0156 | 0.0112 | 0.0279 | 0.007 | 0.0097 | 0.006 | 0.0037 | 0.0174 | 0.023 | 0.0072 | 0.0069 | 0.0009 | 0.0036 | 0.0007 |
Endosome | 0.0086 | 0.0025 | 0.0062 | 0.0085 | 0.0258 | 0.0317 | 0.0051 | 0.0018 | 0.0048 | 0.0072 | 0.0114 | 0.0137 | 0.0136 | 0.001 | 0.0033 | 0.0025 | 0.0137 | 0.0015 |
Golgi | 0.0024 | 0.0006 | 0.0025 | 0.0003 | 0.0041 | 0.0128 | 0.0014 | 0.0003 | 0.0006 | 0.0006 | 0.0009 | 0.0035 | 0.0052 | 0.0002 | 0.0005 | 0.0001 | 0.0023 | 0.0005 |
Lipid Particles | 0.005 | 0.001 | 0.0047 | 0.0003 | 0.0053 | 0.0123 | 0.0021 | 0.0007 | 0.004 | 0.0008 | 0.0094 | 0.0001 | 0.0097 | 0.0002 | 0.001 | 0.0007 | 0.0029 | 0.0219 |
Mitochondria | 0.0265 | 0.0015 | 0.0097 | 0.0007 | 0.0063 | 0.0606 | 0.006 | 0.0004 | 0.0034 | 0.001 | 0.0297 | 0.0054 | 0.0129 | 0.0008 | 0.0096 | 0.0018 | 0.0017 | 0.1146 |
None | 0.0033 | 0.0002 | 0.0055 | 0.0005 | 0.0051 | 0.053 | 0.0043 | 0.0003 | 0.0043 | 0.0003 | 0.0075 | 0.0008 | 0.0073 | 0.0003 | 0.0019 | 0.0005 | 0.0074 | 0.007 |
Nuclear Periphery | 0.0408 | 0.0063 | 0.0145 | 0.016 | 0.0307 | 0.005 | 0.0498 | 0.0035 | 0.0088 | 0.0172 | 0.0085 | 0.0026 | 0.0269 | 0.0045 | 0.0108 | 0.0125 | 0.0138 | 0.0025 |
Nucleolus | 0.0107 | 0.0001 | 0.0072 | 0.0004 | 0.0019 | 0.1284 | 0.0108 | 0.0001 | 0.0013 | 0.0007 | 0.0201 | 0 | 0.0031 | 0.0002 | 0.0064 | 0.0027 | 0.0047 | 0.0492 |
Nucleus | 0.0113 | 0.0023 | 0.0066 | 0.0054 | 0.0083 | 0.0308 | 0.0205 | 0.0025 | 0.0053 | 0.01 | 0.0119 | 0.0015 | 0.0162 | 0.0056 | 0.007 | 0.0141 | 0.0115 | 0.0066 |
Peroxisomes | 0.0029 | 0.0001 | 0.0021 | 0.0001 | 0.0012 | 0.0034 | 0.0004 | 0 | 0.0004 | 0.0001 | 0.0023 | 0.0001 | 0.0044 | 0 | 0.0006 | 0.0002 | 0.0011 | 0.0097 |
Punctate Nuclear | 0.0022 | 0 | 0.0011 | 0 | 0.0052 | 0.0034 | 0.0009 | 0 | 0.0015 | 0.0001 | 0.0036 | 0 | 0.0091 | 0 | 0.0002 | 0.0002 | 0.0061 | 0.0026 |
Vacuole | 0.1121 | 0.0452 | 0.1329 | 0.2193 | 0.0781 | 0.0857 | 0.1065 | 0.0368 | 0.0984 | 0.2011 | 0.1306 | 0.0078 | 0.0662 | 0.021 | 0.0727 | 0.1337 | 0.1016 | 0.0166 |
Vacuole Periphery | 0.0359 | 0.0056 | 0.0303 | 0.0715 | 0.0456 | 0.0508 | 0.026 | 0.0055 | 0.0188 | 0.0591 | 0.055 | 0.0046 | 0.0177 | 0.0034 | 0.0223 | 0.0571 | 0.049 | 0.0059 |
R1 | R2 | |||||||||
---|---|---|---|---|---|---|---|---|---|---|
G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | |
Gene Expression | 2021.477 | 2365.5117 | 2700.8448 | 2173.2994 | 2594.9559 | 2218.4603 | 1992.3842 | 1832.1087 | 2071.7408 | 1801.798 |
Translational Efficiency | 1.5129 | 1.4099 | 1.3121 | 1.4838 | 1.4138 | 1.308 | 1.3572 | 1.4977 | 1.2613 | 1.5498 |
Dataset | Hit |
---|---|
Protein Concentration | ✘ |
Protein Localization | ✘ |
Gene Expression | ✘ |
Translational Efficiency | ✘ |
R1 | R2 | R1 & R2 | |||||||||
---|---|---|---|---|---|---|---|---|---|---|---|
WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 |
952 | 123 | 2000 | 1930 | 1381 | 1588 | 1804 | 1889 | 2333 | 1711 | 3804 | 3819 |
R1 | R2 | R1 & R2 | ||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|
WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 | |
Mean | 6997.73 | 10613.71 | 14486.25 | 13150.04 | 7807.01 | 8332.59 | 14494.76 | 14274.36 | 7476.78 | 8496.57 | 14490.29 | 13706.16 |
Standard Deviation | 2024.27 | 1884.44 | 2344.35 | 3657.04 | 1558.75 | 1918.39 | 2606.43 | 3216.28 | 1807.90 | 2004.53 | 2472.11 | 3491.63 |
Intensity Change Log 2 | — | 0.600970 | 1.049725 | 0.910108 | — | 0.093995 | 0.892689 | 0.870584 | — | 0.355857 | 0.969049 | 0.889401 |
R1 | R2 | R1 & R2 | ||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|
WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 | |
Actin | 0.000075 | 0.000108 | 0.000109 | 0.000178 | 0.000037 | 0.000057 | 0.000100 | 0.000134 | 0.000052 | 0.000060 | 0.000104 | 0.000156 |
Bud Neck | 0.000426 | 0.001670 | 0.000407 | 0.000908 | 0.000362 | 0.000413 | 0.000713 | 0.000763 | 0.000388 | 0.000503 | 0.000552 | 0.000836 |
Bud Site | 0.002301 | 0.003152 | 0.002143 | 0.005205 | 0.001149 | 0.002447 | 0.003394 | 0.006429 | 0.001619 | 0.002498 | 0.002736 | 0.005810 |
Cell Periphery | 0.024012 | 0.059962 | 0.056549 | 0.028343 | 0.012605 | 0.011036 | 0.039940 | 0.030064 | 0.017260 | 0.014553 | 0.048672 | 0.029194 |
Cytoplasm | 0.664372* | 0.463777* | 0.521249* | 0.638862* | 0.658924* | 0.788446* | 0.583382* | 0.611884* | 0.661147* | 0.765106* | 0.550715* | 0.625518* |
Cytoplasmic Foci | 0.009474 | 0.014515 | 0.003712 | 0.004646 | 0.008245 | 0.007660 | 0.003605 | 0.004837 | 0.008746 | 0.008153 | 0.003661 | 0.004740 |
Eisosomes | 0.000002 | 0.000004 | 0.000004 | 0.000003 | 0.000001 | 0.000001 | 0.000003 | 0.000002 | 0.000001 | 0.000002 | 0.000003 | 0.000003 |
Endoplasmic Reticulum | 0.001512 | 0.001424 | 0.001404 | 0.000380 | 0.000464 | 0.000395 | 0.000558 | 0.000599 | 0.000892 | 0.000469 | 0.001003 | 0.000488 |
Endosome | 0.000765 | 0.000635 | 0.000439 | 0.000956 | 0.000452 | 0.000429 | 0.000352 | 0.000515 | 0.000580 | 0.000443 | 0.000398 | 0.000738 |
Golgi | 0.072526 | 0.094216 | 0.022536 | 0.011965 | 0.072126 | 0.041022 | 0.019748 | 0.025071 | 0.072289 | 0.044846 | 0.021214 | 0.018448 |
Lipid Particles | 0.021255 | 0.027568 | 0.026881 | 0.011523 | 0.009047 | 0.006055 | 0.022617 | 0.016131 | 0.014028 | 0.007601 | 0.024859 | 0.013802 |
Mitochondria | 0.000160 | 0.000108 | 0.000365 | 0.001031 | 0.000089 | 0.000235 | 0.000224 | 0.000311 | 0.000118 | 0.000226 | 0.000298 | 0.000675 |
Mitotic Spindle | 0.001131 | 0.001612 | 0.000360 | 0.000548 | 0.000675 | 0.001125 | 0.000306 | 0.000551 | 0.000861 | 0.001160 | 0.000334 | 0.000549 |
None | 0.005399 | 0.003516 | 0.000955 | 0.004170 | 0.002001 | 0.003751 | 0.000870 | 0.001378 | 0.003388 | 0.003735 | 0.000915 | 0.002789 |
Nuclear Periphery | 0.000913 | 0.003338 | 0.000836 | 0.000339 | 0.000957 | 0.000463 | 0.000676 | 0.000444 | 0.000939 | 0.000670 | 0.000760 | 0.000391 |
Nuclear Periphery Foci | 0.017293 | 0.030817 | 0.007497 | 0.003841 | 0.018373 | 0.009130 | 0.006695 | 0.005444 | 0.017932 | 0.010689 | 0.007117 | 0.004634 |
Nucleolus | 0.000119 | 0.000098 | 0.000113 | 0.000242 | 0.000065 | 0.000083 | 0.000104 | 0.000158 | 0.000087 | 0.000085 | 0.000109 | 0.000200 |
Nucleus | 0.010891 | 0.014712 | 0.005074 | 0.013099 | 0.009799 | 0.009642 | 0.007989 | 0.007957 | 0.010245 | 0.010007 | 0.006456 | 0.010556 |
Peroxisomes | 0.000015 | 0.000019 | 0.000125 | 0.000204 | 0.000006 | 0.000015 | 0.000046 | 0.000086 | 0.000009 | 0.000015 | 0.000088 | 0.000145 |
Vacuole | 0.085094 | 0.186533 | 0.281425* | 0.234913* | 0.091010 | 0.066725 | 0.259402* | 0.224252* | 0.088596 | 0.075338 | 0.270981* | 0.229640* |
Vacuole Periphery | 0.082264 | 0.092216 | 0.067817 | 0.038647 | 0.113613* | 0.050868 | 0.049276 | 0.062990 | 0.100821* | 0.053840 | 0.059024 | 0.050688 |
R1 | R2 | R1 & R2 | |||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
UBP2_WT | UBP14_WT | UBP2UBP14_WT | UBP2UBP14_UBP2 | UBP2UBP14_UBP14 | UBP2_WT | UBP14_WT | UBP2UBP14_WT | UBP2UBP14_UBP2 | UBP2UBP14_UBP14 | UBP2_WT | UBP14_WT | UBP2UBP14_WT | UBP2UBP14_UBP2 | UBP2UBP14_UBP14 | |
Actin | -1.82 | -1.46 | -4.35 | -2.44 | -1.91 | -2.26 | -5.61 | -2.42 | -2.13 | -1.34 | -1.08 | -3.71 | -3.95 | -3.63 | -2.08 |
Bud Neck | -3.01 | -0.11 | -6.59 | 1.02 | -6.70 | -0.92 | -4.95 | -5.57 | -3.58 | -0.44 | -1.86 | -4.22 | -8.63 | -4.76 | -5.50 |
Bud Site | -1.36 | 0.28 | -6.87 | -3.12 | -7.12 | -2.57 | -4.44 | -9.12 | -5.34 | -3.99 | -1.98 | -4.19 | -11.75 | -5.94 | -7.31 |
Cell Periphery | -4.36 | -13.18 | -0.85 | 4.16 | 12.97 | 1.42 | -14.84 | -10.98 | -11.58 | 4.70 | 2.21 | -20.90 | -8.28 | -9.38 | 12.91 |
Cytoplasm | 5.87 | 10.59 | 2.53 | -5.03 | -10.12 | -10.84 | 6.56 | 4.25 | 16.66 | -2.56 | -9.89 | 12.50 | 4.71 | 15.62 | -9.05 |
Cytoplasmic Foci | -1.88 | 6.74 | 3.60 | 3.12 | -2.28 | 0.53 | 6.79 | 4.25 | 3.66 | -2.23 | 0.44 | 9.62 | 5.34 | 4.23 | -3.15 |
Eisosomes | -3.20 | -1.42 | -4.27 | 1.24 | 0.40 | -1.19 | -7.39 | -6.08 | -3.01 | -0.04 | -0.93 | -2.59 | -7.55 | -3.57 | 0.40 |
Endoplasmic Reticulum | 0.11 | 0.51 | 5.11 | 1.06 | 8.24 | 0.93 | -1.30 | -1.58 | -2.27 | -0.62 | 3.26 | -0.81 | 4.09 | -0.02 | 6.84 |
Endosome | 1.13 | 4.30 | -0.43 | -0.86 | -1.61 | 0.12 | 2.45 | -1.28 | -1.24 | -3.68 | 2.12 | 4.35 | -0.89 | -1.70 | -2.23 |
Golgi | -2.16 | 13.85 | 17.05 | 9.51 | 10.88 | 9.70 | 19.25 | 16.70 | 7.47 | -3.59 | 9.82 | 23.44 | 24.60 | 13.51 | 3.63 |
Lipid Particles | -1.64 | -2.55 | 4.91 | 4.49 | 11.88 | 3.91 | -10.40 | -5.93 | -9.29 | 4.33 | 6.37 | -9.13 | 0.39 | -7.65 | 11.25 |
Mitochondria | 1.70 | -2.67 | -2.36 | -2.49 | -1.69 | -2.90 | -2.84 | -4.22 | -1.34 | -1.31 | -2.30 | -3.90 | -3.04 | -2.38 | -1.90 |
Mitotic Spindle | -0.93 | 3.46 | 1.68 | 1.82 | -2.28 | -1.64 | 5.94 | -0.16 | 0.99 | -1.13 | -1.26 | 5.40 | 0.63 | 1.48 | -1.85 |
None | 1.73 | 4.75 | 1.15 | -0.63 | -4.15 | -2.36 | 8.18 | 2.06 | 3.00 | -1.80 | -0.41 | 6.26 | 1.18 | 1.24 | -4.50 |
Nuclear Periphery | -1.84 | 0.71 | 6.22 | 2.24 | 11.38 | 7.17 | 3.84 | 7.53 | 0.03 | 5.39 | 2.11 | 2.92 | 10.01 | 2.52 | 11.96 |
Nuclear Periphery Foci | -2.23 | 8.07 | 11.33 | 4.52 | 9.19 | 7.69 | 11.37 | 12.43 | 4.85 | 2.74 | 6.70 | 13.77 | 17.06 | 7.51 | 8.30 |
Nucleolus | 0.74 | 0.34 | -4.72 | -5.45 | -6.15 | -1.63 | -6.86 | -6.68 | -3.62 | -4.00 | -0.18 | -2.40 | -7.89 | -6.35 | -7.30 |
Nucleus | -1.14 | 4.77 | -3.91 | -0.29 | -9.54 | 0.14 | 0.80 | 0.37 | 0.28 | -0.42 | -0.04 | 4.17 | -2.97 | -2.93 | -7.77 |
Peroxisomes | -0.59 | -7.30 | -2.50 | -2.42 | -0.88 | -2.19 | -8.49 | -10.91 | -8.05 | -4.69 | -1.53 | -9.39 | -3.62 | -3.40 | -1.37 |
Vacuole | -5.34 | -26.44 | -20.58 | -2.63 | 4.86 | 4.53 | -23.69 | -19.70 | -23.87 | 4.28 | 2.57 | -35.74 | -28.57 | -28.90 | 6.53 |
Vacuole Periphery | -0.81 | 3.03 | 9.87 | 5.63 | 13.02 | 11.79 | 13.19 | 10.21 | -3.16 | -4.78 | 11.33 | 11.87 | 14.70 | 1.83 | 5.51 |
Temp | Actin Patch (Sac6-tdTomato) | Cortical Patch (Sla1-GFP) | Late Endosome (Snf7-GFP) | Vacuole (Vph1-GFP) |
---|---|---|---|---|
37℃ | ||||
RT |
Gene / Allele | Actin Patch (Sac6-tdTomato) | Cortical Patch (Sla1-GFP) | Late Endosome (Snf7-GFP) | Vacuole (Sac6-tdTomato) |
---|
Gene | Images |
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Gene | Images |
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