Standard name
Human Ortholog
Description Transporter of the ATP-binding cassette (ABC) family; involved in bile acid transport; negative regulator of vacuole fusion; regulates release of lumenal Ca2+ stores; similar to mammalian bile transporters; YBT1 has a paralog, VMR1, that arose from the whole genome duplication
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Micrographs

ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 – – – – – –
Cell Periphery 0 0 0 0 0 0 0 0 0 0 0 0.05 0 0 0 0 0 0 0 0 0 0 0 0
Cytoplasm 0 0 0.05 0 0.05 0.1 0.1 0.07 0.14 0.12 0.18 0.13 0 0 0 0.08 0.06 0.06 0 0 0 0 0 0
Endoplasmic Reticulum 0 0 0 0 0 0.05 0.08 0.1 0.11 0.09 0.05 0.09 0 0 0 0 0 0.05 0 0 0 0 0 0
Endosome 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Golgi 0 0 0 0 0.1 0.05 0 0 0 0 0 0 0 0 0 0.1 0.14 0.17 0 0 0 0 0 0
Mitochondria 0.1 0 0.16 0.26 0.13 0.3 0.42 0.46 0.52 0.52 0.56 0.6 0 0 0 0 0 0 0 0 0 0 0 0
Nucleus 0.11 0.11 0.05 0 0 0 0 0 0 0 0 0 0.06 0 0 0 0 0 0 0.06 0 0.05 0 0
Nuclear Periphery 0.1 0.29 0.16 0.36 0.16 0.21 0.27 0.14 0.13 0.1 0 0.12 0.05 0.2 0.21 0.17 0.26 0.24 0 0.08 0.09 0.05 0 0
Nucleolus 0.05 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Vac/Vac Membrane 0.83 0.79 0.78 0.72 0.72 0.53 0.42 0.41 0.36 0.35 0.37 0.33 0.91 0.91 0.91 0.83 0.8 0.86 0.9 0.84 0.8 0.84 0.87 0.89
ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 1 0 0 1 3 9 4 6 1 4 0 0 0 0 1 0 0 0 0 0 0 0
Bud 0 0 0 0 0 2 1 0 1 1 4 4 0 0 0 0 0 1 0 0 0 0 1 4
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0 0 1 0 0 0 0 0 0 – – – – – –
Cell Periphery 0 0 0 1 0 7 17 15 22 26 10 30 0 2 2 10 7 7 0 0 0 0 0 0
Cytoplasm 0 0 6 12 20 50 62 44 75 71 70 79 17 6 13 26 14 18 0 0 0 0 0 2
Endoplasmic Reticulum 0 0 0 0 10 24 51 59 57 53 18 55 0 1 1 14 7 15 0 0 1 0 0 0
Endosome 0 0 0 0 4 1 2 3 1 0 3 1 0 1 4 1 1 1 0 0 0 0 1 5
Golgi 2 0 2 7 41 24 26 14 4 6 2 5 18 2 6 30 30 52 0 0 0 0 1 1
Mitochondria 10 0 20 107 56 151 265 281 279 309 215 372 12 12 6 7 8 8 1 0 5 0 5 0
Nucleus 11 3 7 4 10 18 16 11 4 12 3 5 40 10 19 5 5 7 3 3 5 3 15 21
Nuclear Periphery 10 8 20 146 67 104 168 86 67 62 16 72 36 102 135 54 57 71 4 4 12 3 14 14
Nucleolus 5 1 2 17 15 10 5 7 1 4 2 4 4 16 18 4 4 2 0 0 0 1 6 3
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 1 0 1 0 1 0 0 0 0 0 0 0 0 0 0 0 0
Vac/Vac Membrane 83 22 100 291 303 266 265 252 195 209 142 206 611 464 580 261 176 258 98 45 115 65 332 476
Unique Cell Count 100 28 129 405 419 498 631 612 535 594 383 620 672 510 639 313 221 301 110 54 145 79 381 533
Labelled Cell Count 121 34 158 585 526 658 881 782 710 760 486 839 738 616 784 412 310 440 110 54 145 79 381 533


Vacuole Periphery

(Huh et al. Nature 2003 425:686)
Screen WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 AF100 AF140 AF180
Mean Cell GFP Intensity (1e-4) 14.9 19.7 9.3 13.1 12.8 10.1 8.9 8.7 8.3 8.5 8.0 7.7 17.8 17.5 16.5 25.1 23.7 24.2 13.6 17.5 18.1
Std Deviation (1e-4) 3.4 4.5 2.7 3.0 3.0 2.7 2.2 1.9 2.1 2.0 1.9 1.8 4.6 4.2 4.1 8.6 7.5 8.3 3.7 4.8 5.0
Intensity Change (Log2) – – – 0.49 0.46 0.12 -0.06 -0.1 -0.17 -0.13 -0.22 -0.28 0.93 0.91 0.82 1.43 1.35 1.38 0.54 0.91 0.96


Localization RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3
Cortical Patches 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cell Periphery 0 0 0 0 0 0 0 0 2.5 0 0 0 0 0 0
Cytoplasm 0 0.1 1.9 1.9 1.0 2.9 2.4 3.8 2.6 0 0 0 1.3 0.7 0.6
Endoplasmic Reticulum 0 0 0 3.3 3.7 3.9 3.5 0 3.5 0 0 0 0 0 0
Endosome 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Golgi 0 3.0 1.7 0 0 0 0 0 0 0 0 0 3.0 3.8 4.5
Mitochondria 2.5 -0.6 3.4 5.7 6.4 7.5 7.5 8.0 9.2 -7.3 -6.1 0 -5.3 -4.0 -4.9
Nucleus 0 0 -0.9 0 0 0 0 0 0 0.2 0 0 0 0 0
Nuclear Periphery 4.4 0.1 1.4 2.7 -0.4 -0.9 -1.6 -4.4 -1.2 -4.1 1.2 1.5 0.4 2.2 1.9
Nucleolus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Vacuole -1.3 -1.2 -4.9 -7.4 -7.5 -8.4 -8.8 -8.0 -9.3 4.4 4.2 4.3 1.4 0.5 2.1

Images

Circle plot not available

Protein Concentration and Protein Localization Data

R1 R2 R3
G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase
Concentration 12.1143 16.1032 13.3896 13.6387 12.095 13.9792 12.8958 17.3359 14.2042 13.7962 13.0046 14.2637 13.5887 20.2332 17.149 17.7408 15.8504 17.5397
Actin 0.0119 0.0006 0.0171 0.0088 0.0005 0.0007 0.0002 0 0.0059 0.0003 0.0001 0.0019 0.028 0 0.0066 0 0.0005 0
Bud 0.0002 0.0001 0.0002 0.0002 0 0 0.0001 0 0.0018 0.0013 0.0002 0.0005 0.0007 0 0.0021 0 0 0.0027
Bud Neck 0.0008 0 0.0006 0.0046 0 0.0001 0 0 0.0001 0.0001 0 0.0006 0.0029 0 0.0005 0 0 0
Bud Periphery 0.0003 0.0002 0.0004 0.0002 0.0001 0.0001 0.0003 0.0001 0.0051 0.004 0.0005 0.0016 0.0013 0.0001 0.0015 0.0001 0.0001 0.0017
Bud Site 0.0009 0.0002 0.0013 0.0037 0.0001 0.0001 0.0001 0 0.0006 0.0083 0.0003 0.0015 0.0067 0 0.0139 0 0.0001 0.0002
Cell Periphery 0.0008 0.0005 0.0009 0.0002 0.0002 0.0003 0.0007 0.0003 0.0006 0.0005 0.0001 0.0009 0.0034 0.0004 0.0009 0.0003 0.0003 0.0003
Cytoplasm 0.003 0 0.0019 0.0027 0.0001 0.0003 0.0019 0 0.0056 0.003 0.0017 0.0003 0.0034 0.0001 0.0013 0 0.0005 0.001
Cytoplasmic Foci 0.0103 0.0004 0.0062 0.0112 0.0016 0.0013 0.0003 0 0.0058 0.0059 0.0021 0.0012 0.0134 0 0.0146 0 0.0614 0.0002
Eisosomes 0.0002 0 0.0002 0.0001 0 0 0 0 0 0 0 0.0004 0.0012 0 0 0 0 0
Endoplasmic Reticulum 0.0019 0.0001 0.0016 0.0005 0.0003 0.0004 0.0031 0.0002 0.0012 0.0009 0.0015 0.0008 0.0028 0.0001 0.0004 0.0001 0.0002 0.0001
Endosome 0.0155 0.0023 0.0065 0.006 0.0076 0.0045 0.0185 0.0014 0.0144 0.0152 0.0628 0.0053 0.0141 0.002 0.0129 0.0012 0.0139 0.0014
Golgi 0.0037 0.0015 0.0034 0.0024 0.0018 0.0009 0.0007 0.0001 0.0087 0.0012 0.0025 0.0014 0.0045 0.0001 0.0021 0 0.0034 0.0002
Lipid Particles 0.0135 0.0012 0.0097 0.0111 0.0173 0.002 0.0008 0.0002 0.0021 0.0019 0.0013 0.0056 0.0086 0.0003 0.0033 0.0005 0.0448 0.0058
Mitochondria 0.0085 0.0024 0.0144 0.0052 0.0062 0.0145 0.0022 0.0154 0.0114 0.0025 0.0105 0.0292 0.0303 0.0064 0.0049 0.0006 0.0003 0.0053
None 0.0047 0 0.0036 0.0034 0.0003 0.0003 0.0002 0 0.002 0.0019 0.0003 0.0007 0.0024 0 0.0007 0 0.0005 0
Nuclear Periphery 0.0157 0.006 0.0064 0.0029 0.0056 0.0203 0.0125 0.0173 0.0153 0.0231 0.0123 0.0253 0.0105 0.0073 0.018 0.0141 0.002 0.0207
Nucleolus 0.0078 0.0003 0.001 0.0008 0.0007 0.0033 0.0004 0.0002 0.0006 0.0014 0.0001 0.0071 0.002 0.0085 0.0008 0.0003 0.0001 0.0008
Nucleus 0.0026 0.0004 0.001 0.0008 0.0006 0.0024 0.0012 0.0003 0.0066 0.0028 0.001 0.0052 0.0041 0.0015 0.0013 0.0004 0.0002 0.0019
Peroxisomes 0.0083 0.0003 0.0103 0.01 0.0026 0.0004 0 0 0.0007 0.0008 0.0002 0.002 0.0099 0 0.0152 0 0.0152 0.0001
Punctate Nuclear 0.004 0.0002 0.0027 0.0088 0.0012 0.0014 0 0 0.0004 0.0012 0.0001 0.0011 0.0055 0 0.0052 0 0.0025 0
Vacuole 0.1795 0.1877 0.1671 0.1655 0.1192 0.0886 0.2113 0.1596 0.1458 0.1645 0.0977 0.0939 0.2442 0.2316 0.2174 0.1919 0.1831 0.1169
Vacuole Periphery 0.706 0.7955 0.7434 0.7511 0.8339 0.8581 0.7452 0.805 0.7654 0.7592 0.8047 0.8136 0.5999 0.7413 0.6764 0.7903 0.6707 0.8408

Sequencing Data

R1 R2
G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Post-START S/G2 Metaphase Anaphase Telophase
Gene Expression 86.3072 76.7577 81.6196 84.846 68.2883 74.3158 75.4094 81.0695 70.8582 88.1467
Translational Efficiency 0.4617 0.4628 0.4621 0.3539 0.3951 0.4569 0.4041 0.2928 0.4232 0.3853

Hit Data

Dataset Hit
Protein Concentration ✘
Protein Localization ✘
Gene Expression ✘
Translational Efficiency ✘
Standard name
Human Ortholog
Description Transporter of the ATP-binding cassette (ABC) family; involved in bile acid transport; negative regulator of vacuole fusion; regulates release of lumenal Ca2+ stores; similar to mammalian bile transporters; YBT1 has a paralog, VMR1, that arose from the whole genome duplication
Localization
Cell Percentages vacuolar membrane (84%)
Cell Cycle Regulation No
Subcompartmental Group vac memb-2

Ybt1

Ybt1


Nearest neighbours ( genes)

correlation threshold

0.6

1.0

0.80


Biological Process (Gene Ontology)
Molecular Function (Gene Ontology)
Cellular Component (Gene Ontology)

Endocytosis
Temp Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Vph1-GFP)
37℃
RT
Cell Cycle Omics

CYCLoPs (Ybt1-GFP)
Gene / Allele Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Sac6-tdTomato)
Gene Images
Gene Images
Images are not yet available
Images are not yet available