ORF
Human Ortholog
Description Putative protein of unknown function with similarity to Pip2p; an oleate-specific transcriptional activator of peroxisome proliferation; YLL054C is not an essential gene
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Micrographs

ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 – – – – – –
Cell Periphery 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cytoplasm 0.98 0.98 0.99 0.99 0.98 0.96 0.94 – 0.88 0.8 0.75 0.82 1.0 0.99 0.99 0.96 0.97 0.98 0.92 0.89 0.86 0.67 0.66 0.65
Endoplasmic Reticulum 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0.08 0 0.08 0 0 0 0 0 0
Endosome 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Golgi 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Mitochondria 0 0 0 0 0 0.06 0.1 – 0.27 0.35 0.37 0.31 0 0 0 0 0 0 0 0 0.08 0.14 0.13 0.13
Nucleus 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nuclear Periphery 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Vac/Vac Membrane 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0.06 0.06
ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 1 2 0 1 0 – 0 1 0 0 0 0 0 1 0 0 0 1 2 6 10 9
Bud 0 0 1 0 1 0 3 – 2 7 9 4 1 0 0 0 0 0 0 1 0 12 14 12
Bud Neck 0 1 4 5 13 12 12 – 10 5 4 5 0 0 0 7 2 0 0 1 0 2 4 6
Bud Site 0 0 0 0 0 0 0 – 1 0 3 0 0 0 0 0 0 0 – – – – – –
Cell Periphery 0 0 9 6 3 2 1 – 3 2 1 1 0 0 0 8 9 6 0 1 3 3 3 7
Cytoplasm 120 348 319 236 291 358 443 – 347 291 285 283 520 498 665 537 340 397 118 326 271 266 262 285
Endoplasmic Reticulum 1 2 0 1 2 0 0 – 0 0 1 0 0 1 2 45 14 33 0 0 0 3 3 4
Endosome 0 1 0 0 0 0 1 – 0 0 0 1 0 0 0 6 3 3 1 5 3 3 5 6
Golgi 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 1 1 0 3 0 2
Mitochondria 2 7 0 0 0 23 49 – 108 128 140 106 7 2 6 12 3 1 3 15 26 56 53 55
Nucleus 0 0 0 0 3 1 1 – 0 0 1 0 0 2 1 3 2 3 0 0 0 0 0 0
Nuclear Periphery 0 0 0 0 1 0 1 – 1 0 0 0 0 0 0 0 0 0 0 0 0 1 0 0
Nucleolus 0 0 0 0 0 0 0 – 0 0 1 0 2 1 1 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 – 0 0 0 1 0 0 0 0 0 0 0 0 0 0 1 1
SpindlePole 0 0 0 0 1 1 6 – 4 4 9 6 0 0 0 2 2 2 0 1 1 7 6 9
Vac/Vac Membrane 0 0 0 0 0 2 1 – 2 3 3 0 0 1 2 6 8 3 1 5 2 17 22 27
Unique Cell Count 123 356 321 238 298 374 473 396 362 379 345 522 503 674 562 350 404 129 365 317 395 397 440
Labelled Cell Count 123 359 334 250 315 400 518 478 441 457 407 530 505 677 627 383 448 129 365 317 395 397 440


Cytoplasm

(Huh et al. Nature 2003 425:686)
Screen WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 AF100 AF140 AF180
Mean Cell GFP Intensity (1e-4) 3.9 4.2 4.6 4.3 4.9 3.6 3.8 – 3.2 3.1 3.0 3.5 3.9 3.9 4.2 5.7 6.0 5.6 3.8 3.9 4.3
Std Deviation (1e-4) 0.7 0.7 0.7 0.8 1.5 0.8 0.9 – 0.6 0.6 0.5 0.9 1.0 1.0 1.5 1.2 1.5 1.2 1.5 1.6 1.8
Intensity Change (Log2) – – – -0.12 0.07 -0.37 -0.28 – -0.55 -0.56 -0.61 -0.39 -0.25 -0.23 -0.12 0.3 0.37 0.27 -0.27 -0.25 -0.12


Localization RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3
Actin – – – – – – – – – – – – – – –
Bud – – – – – – – – – – – – – – –
Bud Neck – – – – – – – – – – – – – – –
Bud Site – – – – – – – – – – – – – – –
Cell Periphery – – – – – – – – – – – – – – –
Cyto – – – – – – – – – – – – – – –
Endoplasmic Reticulum – – – – – – – – – – – – – – –
Endosome – – – – – – – – – – – – – – –
Golgi – – – – – – – – – – – – – – –
Mitochondria – – – – – – – – – – – – – – –
Nuclear Periphery – – – – – – – – – – – – – – –
Nuc – – – – – – – – – – – – – – –
Nucleolus – – – – – – – – – – – – – – –
Peroxisomes – – – – – – – – – – – – – – –
SpindlePole – – – – – – – – – – – – – – –
Vac – – – – – – – – – – – – – – –
Cortical Patches – – – – – – – – – – – – – – –
Cytoplasm – – – – – – – – – – – – – – –
Nucleus – – – – – – – – – – – – – – –
Vacuole – – – – – – – – – – – – – – –

Images

Circle plot not available

Protein Concentration and Protein Localization Data

R1 R2 R3
G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase
Concentration -0.4455 -0.5605 -0.9201 -1.0467 -1.0125 -1.0284 0.1909 0.1872 0.573 -0.0931 0.9938 0.1405 0.0216 -0.3447 -0.2714 0.1518 0.1481 -0.3844
Actin 0.0479 0.0088 0.0162 0.0217 0.0037 0.0073 0.016 0.0007 0.0233 0.0107 0.0166 0.0077 0.0432 0.003 0.0092 0.0142 0.0057 0.0028
Bud 0.0017 0.002 0.0003 0.0002 0.0021 0.0002 0.0025 0.0033 0.0049 0.0086 0.001 0.0022 0.0018 0.0038 0.0116 0.0006 0.0323 0.002
Bud Neck 0.0289 0.0034 0.0125 0.0125 0.0091 0.0006 0.0008 0.0007 0.0038 0.0004 0.0127 0.002 0.0037 0.0002 0.0041 0.0237 0.0007 0.0017
Bud Periphery 0.0032 0.0032 0.0006 0.0003 0.0053 0.0004 0.0032 0.0032 0.0055 0.0114 0.0011 0.0041 0.0075 0.0034 0.0069 0.0005 0.0064 0.0009
Bud Site 0.0294 0.0039 0.0013 0.0029 0.0165 0.0004 0.0076 0.0216 0.0142 0.0026 0.0289 0.0018 0.0057 0.0109 0.0078 0.009 0.0055 0.0068
Cell Periphery 0.0009 0.0002 0.0004 0.0002 0.0006 0.0001 0.0005 0.0006 0.0005 0.0002 0.0003 0.0002 0.001 0.0004 0.0003 0.0001 0.0004 0.0001
Cytoplasm 0.0391 0.0464 0.0425 0.0686 0.0499 0.0726 0.0862 0.1385 0.122 0.1199 0.1222 0.1564 0.0591 0.1334 0.1012 0.1289 0.1586 0.2049
Cytoplasmic Foci 0.0307 0.0155 0.0043 0.0028 0.0214 0.0162 0.0205 0.0075 0.0075 0.0283 0.0235 0.0127 0.0199 0.0077 0.0055 0.0297 0.0171 0.0096
Eisosomes 0.0009 0.0007 0.0003 0.0005 0.0002 0.0003 0.0007 0.0002 0.0003 0.0001 0.0002 0.0001 0.0005 0.0002 0.0002 0.0001 0.0001 0.0001
Endoplasmic Reticulum 0.0063 0.0011 0.0009 0.0009 0.002 0.0007 0.0044 0.0013 0.0017 0.002 0.0043 0.0011 0.0075 0.0021 0.0019 0.0021 0.0009 0.003
Endosome 0.0224 0.0016 0.002 0.0016 0.0316 0.0031 0.0088 0.0022 0.0115 0.0141 0.0266 0.0024 0.0155 0.0028 0.0065 0.0191 0.0061 0.0062
Golgi 0.0057 0.0006 0.0004 0.0015 0.0037 0.0018 0.0028 0.0002 0.0038 0.0132 0.0103 0.0016 0.008 0.0005 0.0008 0.01 0.0131 0.0036
Lipid Particles 0.0045 0.0012 0.0007 0.0008 0.0032 0.003 0.0071 0.0004 0.0054 0.0099 0.0418 0.0027 0.0135 0.0007 0.0007 0.0139 0.0028 0.0009
Mitochondria 0.005 0.0018 0.0006 0.0005 0.0022 0.0007 0.0033 0.0006 0.0072 0.0198 0.0249 0.0023 0.0067 0.0005 0.001 0.0025 0.0147 0.0016
None 0.7273 0.9038 0.912 0.8811 0.8186 0.8858 0.7773 0.8098 0.7735 0.7029 0.5914 0.7884 0.7706 0.8225 0.8281 0.7212 0.7261 0.7454
Nuclear Periphery 0.0221 0.0006 0.0004 0.0006 0.0028 0.0008 0.0165 0.0007 0.0026 0.0026 0.0346 0.0018 0.0108 0.0008 0.0036 0.0012 0.0004 0.001
Nucleolus 0.001 0.0006 0.0005 0.0003 0.0029 0.0003 0.0018 0.0008 0.0014 0.0008 0.0039 0.0009 0.0005 0.0005 0.0007 0.0005 0.0002 0.0003
Nucleus 0.007 0.001 0.0012 0.0006 0.0056 0.0005 0.0164 0.0033 0.0023 0.0036 0.0097 0.0035 0.004 0.0021 0.0019 0.0023 0.0024 0.0023
Peroxisomes 0.0067 0.0007 0.0005 0.0005 0.0013 0.0015 0.0071 0.0004 0.002 0.0398 0.0227 0.0012 0.011 0.0007 0.0006 0.0137 0.0034 0.002
Punctate Nuclear 0.0044 0.0017 0.0009 0.0013 0.0058 0.0032 0.0122 0.0022 0.0029 0.0025 0.0174 0.0055 0.0059 0.0028 0.0059 0.0047 0.0019 0.0039
Vacuole 0.003 0.0009 0.0011 0.0005 0.0103 0.0003 0.0029 0.0014 0.0026 0.0053 0.0026 0.001 0.0023 0.0009 0.0011 0.0014 0.0007 0.0006
Vacuole Periphery 0.002 0.0002 0.0001 0.0001 0.0014 0.0001 0.0012 0.0002 0.0012 0.0011 0.0033 0.0004 0.0012 0.0001 0.0004 0.0006 0.0003 0.0002

Sequencing Data

R1 R2
G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Post-START S/G2 Metaphase Anaphase Telophase
Gene Expression 9.5872 6.6226 8.6722 7.4975 6.7802 7.4042 9.8472 7.8425 7.4242 10.0088
Translational Efficiency – – – – – – – – – –

Hit Data

Dataset Hit
Protein Concentration ✘
Protein Localization ✘
Gene Expression ✘
Translational Efficiency –

Micrographs

Cell Count

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
2451 1136 1287 1525 784 1873 969 448 3235 3009 2256 1973

Protein Abundance

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
Mean 647.29 737.39 821.69 819.42 653.99 697.91 808.23 1122.79 648.91 712.82 815.91 888.30
Standard Deviation 67.73 92.06 92.21 95.80 104.11 80.65 93.46 136.47 78.17 87.26 92.99 165.75
Intensity Change Log 2 — 0.188016 0.344182 0.340191 — 0.093772 0.305497 0.779748 — 0.141421 0.324870 0.577768

Localization Score (DeepLoc)

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
Actin 0.000119 0.000243 0.000178 0.002431 0.000337 0.000417 0.000130 0.000249 0.000172 0.000351 0.000158 0.001936
Bud Neck 0.019088 0.034555 0.003417 0.007310 0.006885 0.051092 0.002437 0.022661 0.016130 0.044849 0.002996 0.010795
Bud Site 0.004637 0.010182 0.004332 0.027720 0.001200 0.023391 0.004735 0.042886 0.003804 0.018404 0.004505 0.031164
Cell Periphery 0.000185 0.000151 0.000126 0.000126 0.000194 0.000133 0.000148 0.000064 0.000187 0.000140 0.000135 0.000112
Cytoplasm 0.533155* 0.385033* 0.323427* 0.477401* 0.479885* 0.376899* 0.461037* 0.450009* 0.520245* 0.379970* 0.382534* 0.471181*
Cytoplasmic Foci 0.169740 0.251302* 0.013707 0.020460 0.224463* 0.290061* 0.017064 0.003412 0.183002* 0.275428* 0.015149 0.016589
Eisosomes 0.000191 0.000146 0.000040 0.000042 0.000194 0.000179 0.000042 0.000013 0.000192 0.000167 0.000041 0.000036
Endoplasmic Reticulum 0.000807 0.000670 0.002650 0.001880 0.000638 0.000425 0.001937 0.000278 0.000766 0.000517 0.002344 0.001516
Endosome 0.007617 0.036228 0.001899 0.005737 0.009976 0.032469 0.001858 0.000830 0.008189 0.033888 0.001881 0.004623
Golgi 0.001781 0.004824 0.000069 0.001402 0.002310 0.008443 0.000061 0.000228 0.001909 0.007077 0.000066 0.001135
Lipid Particles 0.007846 0.009340 0.001598 0.001432 0.012259 0.006738 0.001188 0.000191 0.008915 0.007720 0.001422 0.001150
Mitochondria 0.003082 0.011837 0.000582 0.002245 0.002895 0.017940 0.000307 0.004481 0.003037 0.015636 0.000464 0.002752
Mitotic Spindle 0.000918 0.009679 0.003285 0.061975 0.002399 0.008655 0.003842 0.023571 0.001277 0.009041 0.003525 0.053254
None 0.006115 0.002973 0.005380 0.004121 0.005852 0.001751 0.007062 0.009457 0.006052 0.002212 0.006102 0.005333
Nuclear Periphery 0.000322 0.000514 0.001780 0.001894 0.000575 0.000341 0.001383 0.000551 0.000384 0.000406 0.001609 0.001589
Nuclear Periphery Foci 0.000201 0.000362 0.000803 0.001274 0.000392 0.000208 0.000636 0.000030 0.000248 0.000266 0.000731 0.000991
Nucleolus 0.001138 0.002774 0.000742 0.000435 0.003249 0.001317 0.000335 0.000588 0.001650 0.001867 0.000567 0.000470
Nucleus 0.193142* 0.112826 0.602433* 0.268514* 0.205405* 0.073194 0.436180* 0.416922* 0.196114* 0.088157 0.531024* 0.302212*
Peroxisomes 0.002722 0.013740 0.000289 0.001645 0.004357 0.020649 0.000157 0.002279 0.003119 0.018040 0.000232 0.001789
Vacuole 0.046353 0.109594 0.033072 0.109800 0.035973 0.082978 0.059339 0.020354 0.043837 0.093027 0.044354 0.089489
Vacuole Periphery 0.000840 0.003028 0.000191 0.002158 0.000561 0.002721 0.000123 0.000945 0.000773 0.002837 0.000161 0.001883

Localization Changes (T-score)

R1 R2 R1 & R2
UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14 UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14 UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14
Actin -2.32 -2.49 -1.98 -1.69 -1.71 -0.30 0.50 0.61 4.24 0.60 -2.26 -0.62 -1.82 -1.31 -1.72
Bud Neck -5.25 10.81 8.97 10.16 -3.77 -18.54 3.87 -3.86 10.70 -5.71 -14.69 11.99 5.78 19.35 -7.33
Bud Site -5.01 0.22 -5.38 -2.37 -5.16 -13.42 -2.11 -4.99 1.05 -4.09 -12.14 -0.30 -7.18 1.34 -6.66
Cell Periphery 1.64 3.47 5.26 3.15 3.12 2.79 3.13 10.18 4.80 6.88 2.82 4.11 7.81 3.94 5.57
Cytoplasm 15.92 28.49 19.70 1.18 -8.81 8.97 7.25 6.11 -0.81 -0.25 21.37 26.89 20.11 0.55 -5.92
Cytoplasmic Foci -10.94 44.18 43.78 36.10 -1.52 -7.34 30.62 32.05 51.40 6.58 -17.52 53.27 53.55 61.88 1.37
Eisosomes 4.50 18.97 18.69 12.38 -1.80 1.17 11.65 15.21 18.95 14.10 3.09 22.13 23.43 19.73 2.93
Endoplasmic Reticulum 1.31 -12.98 -14.73 -16.54 2.62 1.84 -10.89 1.52 -0.72 12.85 3.20 -16.59 -13.92 -17.47 5.14
Endosome -11.77 9.15 6.38 13.85 -2.55 -11.76 7.97 8.94 19.31 3.95 -17.88 11.99 9.51 22.68 -1.80
Golgi -4.05 8.37 4.79 5.93 -2.33 -5.38 2.68 2.59 10.89 -2.13 -8.50 7.24 5.19 11.84 -2.47
Lipid Particles -1.54 10.08 10.57 10.54 1.27 4.01 8.41 9.29 12.89 10.31 1.78 13.23 13.92 15.33 3.20
Mitochondria -4.98 5.88 3.40 6.03 -5.96 -9.31 2.83 0.64 11.10 -4.13 -11.24 6.60 3.17 13.12 -7.67
Mitotic Spindle -3.89 -2.24 -9.70 -6.47 -8.87 -3.35 -0.94 -3.31 -1.67 -2.81 -6.08 -2.55 -10.21 -7.27 -9.22
None 3.95 1.56 1.75 -3.45 -0.32 3.71 0.60 -4.27 -13.38 -6.49 6.64 1.60 -0.69 -12.07 -2.86
Nuclear Periphery -3.43 -21.48 -20.86 -19.44 -8.32 1.98 -15.61 -7.93 -7.89 2.47 -0.38 -27.15 -21.81 -19.70 -8.22
Nuclear Periphery Foci -3.67 -6.27 -8.89 -7.57 -2.74 3.28 -5.68 7.25 3.60 11.12 -0.75 -8.55 -8.21 -7.89 -2.09
Nucleolus -3.76 2.33 3.91 4.87 1.27 2.56 4.07 2.97 0.99 -2.78 -0.81 5.22 4.36 5.04 -1.66
Nucleus 14.20 -47.78 -17.85 -26.52 27.96 17.70 -22.82 -19.46 -31.86 -1.25 27.65 -51.00 -24.49 -42.02 22.06
Peroxisomes -8.30 6.62 4.89 10.19 -2.80 -11.12 4.57 1.99 12.27 -1.33 -16.06 8.09 4.88 19.11 -2.61
Vacuole -13.72 -3.20 -24.98 -11.83 -20.96 -14.29 -12.09 -2.25 7.18 8.27 -18.62 -10.45 -23.89 -13.02 -15.50
Vacuole Periphery -7.06 8.11 0.27 4.48 -1.33 -8.16 8.61 1.55 8.20 -1.89 -9.95 9.96 0.41 5.82 -1.55
ORF
Human Ortholog
Description Putative protein of unknown function with similarity to Pip2p; an oleate-specific transcriptional activator of peroxisome proliferation; YLL054C is not an essential gene
Localization
Cell Percentages cytoplasm (22%)
Cell Cycle Regulation No
Subcompartmental Group cytoplasm-9

YLL054C

YLL054C


Nearest neighbours ( genes)

correlation threshold

0.6

1.0

0.80


Biological Process (Gene Ontology)
Molecular Function (Gene Ontology)
Cellular Component (Gene Ontology)

Endocytosis
Temp Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Vph1-GFP)
37℃
RT
Cell Cycle Omics

CYCLoPs (Yll054c-GFP)
Gene / Allele Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Sac6-tdTomato)
Gene Images
Gene Images
Images are not yet available
Images are not yet available