Standard name
Human Ortholog
Description S-methylmethionine-homocysteine methyltransferase; functions along with Sam4p in the conversion of S-adenosylmethionine (AdoMet) to methionine to control the methionine/AdoMet ratio
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Micrographs

ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 0.05 0 0 0.05 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 – – – – – –
Cell Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0.05 0 0 0 0 0 0 0 0
Cytoplasm 0.98 0.98 0.96 1.0 0.99 0.97 0.91 0.91 0.85 0.87 0.78 0.81 0.98 0.99 0.99 0.98 0.97 0.97 0.7 0.78 0.7 0.8 0.78 0.83
Endoplasmic Reticulum 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0.08 0.06 0.07 0 0 0 0 0 0
Endosome 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Golgi 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Mitochondria 0 0 0 0 0 0.07 0.14 0.1 0.26 0.18 0.39 0.23 0 0 0 0 0 0 0.15 0.11 0.15 0.06 0.05 0.05
Nucleus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nuclear Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 0 0 0 0 0.05 0 0 0 0 0 0 0 0 0 0 0 0
Vac/Vac Membrane 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 1 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 2 2 5 0 4 2
Bud 1 1 2 0 0 2 5 2 9 9 11 4 0 0 0 0 0 0 1 0 2 2 11 4
Bud Neck 3 1 11 11 18 12 8 15 7 10 1 7 3 2 3 2 2 3 1 0 1 1 1 5
Bud Site 0 1 0 0 0 0 3 1 1 1 2 0 0 0 0 0 0 0 – – – – – –
Cell Periphery 3 1 1 2 0 0 1 2 0 0 1 0 3 4 2 12 6 4 2 0 2 0 3 2
Cytoplasm 267 158 262 275 362 308 317 292 380 395 309 224 194 318 418 222 300 214 207 133 173 106 214 268
Endoplasmic Reticulum 0 0 3 0 0 1 0 2 0 0 0 0 0 1 1 18 20 15 5 0 3 0 1 1
Endosome 0 1 0 0 0 0 0 0 0 0 0 0 0 1 0 0 0 0 2 4 3 2 3 3
Golgi 0 1 0 0 0 0 0 0 0 0 0 0 0 0 0 1 5 1 0 1 0 0 0 1
Mitochondria 8 2 3 1 1 22 47 32 118 80 156 64 0 0 1 5 7 7 43 19 36 7 13 15
Nucleus 0 0 0 1 0 0 2 5 0 0 3 2 2 0 1 1 3 2 0 0 0 0 0 0
Nuclear Periphery 0 0 0 0 0 1 0 0 0 0 1 0 1 0 0 0 0 0 1 0 1 0 0 0
Nucleolus 0 0 3 0 0 0 1 1 0 0 0 0 0 0 0 0 1 2 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 1 0 0 0 0 0
SpindlePole 0 0 1 0 2 2 5 8 10 9 9 14 0 0 0 0 0 0 2 1 3 0 8 8
Vac/Vac Membrane 0 1 2 0 1 1 1 0 1 3 2 1 0 0 0 2 2 4 11 3 2 2 6 5
Unique Cell Count 273 161 274 276 365 319 347 321 449 455 398 276 197 320 421 226 309 221 297 172 248 132 277 325
Labelled Cell Count 282 167 289 290 384 349 390 360 526 507 495 316 203 326 426 263 346 252 297 172 248 132 277 325


Cytoplasm

(Huh et al. Nature 2003 425:686)
Screen WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 AF100 AF140 AF180
Mean Cell GFP Intensity (1e-4) 3.2 4.2 4.5 4.3 4.4 3.3 3.2 3.9 3.0 3.2 2.9 3.3 4.6 4.7 4.7 5.6 6.1 5.5 4.6 4.8 5.3
Std Deviation (1e-4) 0.9 1.0 1.2 1.6 2.0 1.3 1.4 1.5 1.1 1.1 1.0 1.0 1.1 1.0 1.2 1.8 2.2 1.9 1.8 1.4 1.7
Intensity Change (Log2) – – – -0.08 -0.05 -0.44 -0.51 -0.19 -0.58 -0.48 -0.66 -0.45 0.03 0.06 0.07 0.31 0.43 0.29 0.02 0.08 0.23


Localization RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3
Actin – – – – – – – – – – – – – – –
Bud – – – – – – – – – – – – – – –
Bud Neck – – – – – – – – – – – – – – –
Bud Site – – – – – – – – – – – – – – –
Cell Periphery – – – – – – – – – – – – – – –
Cyto – – – – – – – – – – – – – – –
Endoplasmic Reticulum – – – – – – – – – – – – – – –
Endosome – – – – – – – – – – – – – – –
Golgi – – – – – – – – – – – – – – –
Mitochondria – – – – – – – – – – – – – – –
Nuclear Periphery – – – – – – – – – – – – – – –
Nuc – – – – – – – – – – – – – – –
Nucleolus – – – – – – – – – – – – – – –
Peroxisomes – – – – – – – – – – – – – – –
SpindlePole – – – – – – – – – – – – – – –
Vac – – – – – – – – – – – – – – –
Cortical Patches – – – – – – – – – – – – – – –
Cytoplasm – – – – – – – – – – – – – – –
Nucleus – – – – – – – – – – – – – – –
Vacuole – – – – – – – – – – – – – – –

Images

Circle plot not available

Protein Concentration and Protein Localization Data

R1 R2 R3
G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase
Concentration -0.2055 -1.0937 -0.9225 -0.767 -0.6404 -0.9873 -1.0838 -0.4003 -0.4691 -0.8075 -0.8936 -0.5999 -1.0535 -1.266 -0.9463 -0.4656 -1.2911 -1.2867
Actin 0.0288 0.0006 0.007 0.0242 0.0019 0.0027 0.0016 0.0001 0.0004 0.0001 0.0003 0.0006 0.02 0.0007 0.0134 0.0957 0.0064 0.0024
Bud 0.0033 0.0069 0.0055 0.0016 0.0011 0.0029 0.0042 0.0026 0.0029 0 0.0048 0.0062 0.0008 0.0035 0.0027 0.0014 0.0024 0.0002
Bud Neck 0.0153 0.0006 0.0012 0.0002 0.0002 0.0014 0.0005 0.0002 0.0011 0 0.0003 0.008 0.0009 0.0001 0.0058 0.0128 0.0003 0.0014
Bud Periphery 0.0098 0.0065 0.0033 0.008 0.002 0.0028 0.002 0.0009 0.0016 0 0.0016 0.0022 0.0015 0.0085 0.0017 0.0019 0.0045 0.0002
Bud Site 0.016 0.0225 0.0114 0.0042 0.001 0.0006 0.0087 0.0084 0.0081 0 0.0004 0.002 0.01 0.0041 0.0135 0.0018 0.0005 0.0003
Cell Periphery 0.0016 0.0003 0.0002 0.0002 0.0001 0.0001 0.0004 0.0002 0.0002 0 0.0001 0.0001 0.0002 0.0003 0.0008 0.0002 0.0001 0
Cytoplasm 0.0272 0.0121 0.0078 0.0301 0.0047 0.0177 0.0117 0.0158 0.0123 0.0147 0.0135 0.0173 0.0279 0.0564 0.0302 0.0239 0.054 0.0708
Cytoplasmic Foci 0.0323 0.0049 0.0116 0.01 0.0251 0.0103 0.0063 0.0031 0.0022 0.0022 0.0021 0.0049 0.0298 0.0047 0.0151 0.0129 0.0191 0.0028
Eisosomes 0.0009 0.0002 0.0003 0.0003 0.0001 0.0002 0.0002 0.0001 0.0001 0.0001 0.0001 0 0.0004 0.0001 0.0003 0.0003 0.0001 0.0002
Endoplasmic Reticulum 0.0057 0.0004 0.0004 0.004 0.0004 0.001 0.0004 0.0002 0.0003 0.0001 0.0002 0.0002 0.0032 0.0007 0.0023 0.0005 0.0009 0.0005
Endosome 0.0298 0.0004 0.0087 0.0077 0.0353 0.0059 0.0016 0.0002 0.0001 0.0004 0.0001 0.0001 0.0137 0.0008 0.0054 0.0047 0.0068 0.0019
Golgi 0.0094 0.0001 0.0019 0.0019 0.0186 0.002 0.0005 0 0.0001 0 0 0 0.0028 0.0001 0.0017 0.009 0.0022 0.001
Lipid Particles 0.0248 0.0003 0.0064 0.002 0.0483 0.0043 0.0022 0.0001 0.0002 0.0001 0.0001 0.0001 0.006 0.0003 0.0058 0.0031 0.005 0.001
Mitochondria 0.0127 0.0006 0.0025 0.0028 0.0084 0.005 0.0011 0.0003 0.0004 0.0001 0.0003 0.0002 0.0042 0.0006 0.0043 0.0015 0.001 0.001
None 0.7038 0.9302 0.9087 0.8819 0.8173 0.9236 0.9503 0.957 0.9475 0.9806 0.9537 0.9422 0.8113 0.9158 0.8694 0.8151 0.8365 0.9125
Nuclear Periphery 0.0113 0.0005 0.0026 0.0053 0.0008 0.0065 0.0005 0.0003 0.0006 0.0003 0.0008 0.0005 0.0262 0.0003 0.0065 0.0012 0.0022 0.0008
Nucleolus 0.0132 0.0011 0.0017 0.0008 0.0007 0.0006 0.0012 0.0009 0.0024 0.0001 0.0012 0.0009 0.0013 0.0003 0.0014 0.0004 0.0005 0.0002
Nucleus 0.0177 0.0043 0.003 0.0029 0.0006 0.0018 0.0016 0.0042 0.0058 0.0003 0.0127 0.0047 0.0099 0.0006 0.0053 0.0014 0.0013 0.0008
Peroxisomes 0.0147 0.0008 0.0089 0.0022 0.0294 0.001 0.0018 0.0001 0.0002 0.0001 0.0006 0.0002 0.0173 0.0002 0.0055 0.0031 0.0417 0.0006
Punctate Nuclear 0.0125 0.0062 0.0054 0.0085 0.0024 0.0083 0.0025 0.0048 0.0131 0.0007 0.0068 0.0092 0.0083 0.0011 0.0051 0.0082 0.013 0.0011
Vacuole 0.0073 0.0005 0.001 0.0008 0.0008 0.0008 0.0007 0.0003 0.0004 0.0001 0.0002 0.0002 0.0021 0.0008 0.003 0.0006 0.0011 0.0003
Vacuole Periphery 0.0018 0.0001 0.0005 0.0004 0.0008 0.0006 0.0001 0 0.0001 0 0.0001 0 0.0024 0.0001 0.0007 0.0002 0.0004 0.0002

Sequencing Data

R1 R2
G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Post-START S/G2 Metaphase Anaphase Telophase
Gene Expression 9.6194 7.0817 16.5154 28.7735 8.8038 12.4418 4.603 10.3558 17.298 9.6267
Translational Efficiency 1.1864 1.0297 1.1663 1.0675 0.9936 0.832 1.5201 1.0051 1.2318 0.7841

Hit Data

Dataset Hit
Protein Concentration ✘
Protein Localization ✘
Gene Expression ✘
Translational Efficiency ✘

Micrographs

Cell Count

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
262 778 2638 1395 2004 2138 680 1300 2266 2916 3318 2695

Protein Abundance

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
Mean 661.66 620.61 764.35 720.64 617.45 655.66 806.95 736.88 622.56 646.31 773.08 728.47
Standard Deviation 68.66 99.61 92.91 116.08 69.27 86.69 98.34 107.65 70.63 91.64 95.61 112.38
Intensity Change Log 2 — -0.092403 0.208143 0.123189 — 0.086626 0.386157 0.255107 — -0.003207 0.296818 0.188375

Localization Score (DeepLoc)

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
Actin 0.000140 0.000456 0.000098 0.000943 0.000099 0.000413 0.000165 0.000451 0.000104 0.000424 0.000112 0.000706
Bud Neck 0.023092 0.045783 0.006406 0.011224 0.016662 0.050657 0.002418 0.007566 0.017405 0.049356 0.005589 0.009459
Bud Site 0.003172 0.022044 0.008816 0.053694 0.003390 0.019277 0.004324 0.025014 0.003365 0.020015 0.007896 0.039859
Cell Periphery 0.000181 0.000167 0.000109 0.000536 0.000125 0.000154 0.000187 0.000158 0.000132 0.000158 0.000125 0.000354
Cytoplasm 0.381186* 0.371467* 0.354056* 0.472088* 0.467509* 0.353241* 0.332838* 0.523358* 0.457528* 0.358104* 0.349708* 0.496819*
Cytoplasmic Foci 0.302864* 0.280428* 0.004410 0.027266 0.225308* 0.261174* 0.021179 0.021843 0.234275* 0.266311* 0.007846 0.024650
Eisosomes 0.000250 0.000158 0.000016 0.000064 0.000182 0.000168 0.000068 0.000039 0.000190 0.000165 0.000026 0.000052
Endoplasmic Reticulum 0.000762 0.001017 0.001152 0.002142 0.000793 0.000782 0.002370 0.001190 0.000790 0.000845 0.001401 0.001683
Endosome 0.014099 0.032433 0.000699 0.005132 0.006366 0.029577 0.002904 0.003441 0.007260 0.030339 0.001151 0.004316
Golgi 0.001419 0.008884 0.000046 0.003771 0.002529 0.008143 0.000081 0.003900 0.002400 0.008341 0.000054 0.003834
Lipid Particles 0.012698 0.011248 0.000400 0.001448 0.009850 0.009384 0.002260 0.000995 0.010180 0.009882 0.000781 0.001229
Mitochondria 0.011122 0.016207 0.000827 0.003281 0.005763 0.024746 0.000564 0.001854 0.006382 0.022468 0.000773 0.002593
Mitotic Spindle 0.000126 0.004059 0.003968 0.020800 0.001065 0.010054 0.004206 0.017165 0.000957 0.008455 0.004017 0.019046
None 0.001769 0.009481 0.004710 0.003692 0.002504 0.003230 0.010807 0.009578 0.002419 0.004898 0.005960 0.006531
Nuclear Periphery 0.000799 0.000470 0.001663 0.001527 0.000430 0.000496 0.001681 0.001123 0.000472 0.000489 0.001667 0.001332
Nuclear Periphery Foci 0.000500 0.000538 0.000165 0.001574 0.000182 0.000353 0.001109 0.000541 0.000219 0.000402 0.000359 0.001076
Nucleolus 0.001252 0.002849 0.000367 0.000475 0.000889 0.002384 0.001226 0.000565 0.000931 0.002508 0.000543 0.000518
Nucleus 0.190639* 0.099311 0.594916* 0.291121* 0.222799* 0.118836 0.553345* 0.301770* 0.219081* 0.113627 0.586396* 0.296258*
Peroxisomes 0.012084 0.024913 0.000138 0.000673 0.006196 0.025246 0.000361 0.002026 0.006877 0.025157 0.000184 0.001326
Vacuole 0.040687 0.064414 0.016840 0.094340 0.026763 0.078798 0.057619 0.075940 0.028373 0.074960 0.025198 0.085465
Vacuole Periphery 0.001159 0.003672 0.000198 0.004209 0.000596 0.002887 0.000286 0.001482 0.000661 0.003097 0.000216 0.002894

Localization Changes (T-score)

R1 R2 R1 & R2
UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14 UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14 UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14
Actin -5.48 0.52 -1.83 -0.70 -1.91 -6.43 -4.24 -3.50 0.91 -1.10 -8.36 -2.97 -2.69 -0.50 -2.38
Bud Neck -3.76 3.41 2.88 9.29 -1.80 -13.87 11.44 7.39 18.85 -3.48 -14.52 9.08 7.34 20.60 -1.99
Bud Site -7.96 -3.27 -8.32 -3.11 -6.98 -10.36 -0.84 -5.85 1.06 -4.86 -12.82 -3.90 -10.16 -2.43 -7.90
Cell Periphery 0.79 4.43 -1.52 -1.67 -2.21 -3.34 -5.06 -0.15 2.66 4.43 -3.29 1.50 -1.92 -1.48 -2.11
Cytoplasm 0.64 3.65 -1.54 -2.99 -9.19 13.92 16.84 4.01 -7.65 -12.06 13.56 20.55 5.31 -7.99 -14.61
Cytoplasmic Foci 1.49 22.21 20.72 31.85 -12.80 -5.31 41.65 42.50 48.93 0.05 -5.25 50.19 46.53 58.03 -13.25
Eisosomes 3.62 9.87 7.62 7.76 -8.71 1.19 9.98 14.64 13.79 7.33 2.35 18.88 14.91 14.48 -8.38
Endoplasmic Reticulum -0.64 -2.59 -4.92 -6.02 -5.11 0.06 -12.36 -9.73 -8.54 5.78 -0.42 -10.65 -11.94 -10.70 -2.89
Endosome -5.29 5.51 4.23 11.72 -5.65 -16.91 7.25 8.23 19.96 1.70 -18.59 13.82 7.97 22.69 -6.04
Golgi -6.42 3.99 -0.65 4.92 -2.37 -7.82 7.76 0.74 5.90 -2.16 -9.47 8.33 0.73 7.86 -3.22
Lipid Particles 0.61 5.28 4.82 8.12 -12.49 0.54 12.65 15.58 14.34 7.40 0.40 16.56 15.74 16.18 -6.66
Mitochondria -0.86 1.87 1.60 6.59 -1.86 -10.45 6.40 5.34 14.33 -2.10 -9.92 5.90 4.54 15.23 -2.31
Mitotic Spindle -2.95 -5.17 -7.31 -4.52 -5.15 -5.99 -2.24 -5.40 -0.78 -2.57 -6.41 -4.32 -8.67 -3.20 -5.88
None -3.68 -5.81 -3.28 2.99 3.54 -1.61 -5.68 -4.76 -3.54 1.26 -3.69 -7.18 -4.99 0.10 0.62
Nuclear Periphery 0.89 -3.67 -3.95 -13.28 -1.20 -1.00 -16.21 -18.24 -14.60 2.30 -0.27 -24.98 -19.48 -18.94 0.19
Nuclear Periphery Foci -0.25 1.40 -3.04 -3.49 -4.96 -2.76 -9.10 -5.62 -3.50 3.29 -3.44 -5.77 -6.84 -5.02 -4.63
Nucleolus -4.12 3.68 3.52 7.29 -0.65 -5.00 -1.06 2.82 6.19 3.76 -6.52 3.94 4.40 8.62 0.71
Nucleus 7.14 -34.25 -10.02 -24.80 32.92 18.41 -28.57 -15.07 -28.48 15.76 20.62 -59.71 -17.98 -37.78 37.56
Peroxisomes -4.36 5.66 5.52 11.58 -2.38 -12.71 11.56 6.92 16.01 -1.23 -14.26 13.02 10.67 20.27 -1.89
Vacuole -5.22 4.31 -18.41 -14.46 -23.48 -19.09 -11.94 -20.73 -10.48 -4.97 -20.51 -6.37 -30.50 -17.32 -25.91
Vacuole Periphery -3.69 2.26 -1.22 1.31 -2.52 -9.90 4.42 -0.50 5.10 -1.54 -10.83 6.94 -1.91 2.93 -2.94
Standard name
Human Ortholog
Description S-methylmethionine-homocysteine methyltransferase; functions along with Sam4p in the conversion of S-adenosylmethionine (AdoMet) to methionine to control the methionine/AdoMet ratio
Localization
Cell Percentages cytoplasm (13%)
Cell Cycle Regulation No
Subcompartmental Group cytoplasm-9

Mht1

Mht1


Nearest neighbours ( genes)

correlation threshold

0.6

1.0

0.80


Biological Process (Gene Ontology)
Molecular Function (Gene Ontology)
Cellular Component (Gene Ontology)

Endocytosis
Temp Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Vph1-GFP)
37℃
RT
Cell Cycle Omics

CYCLoPs (Mht1-GFP)
Gene / Allele Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Sac6-tdTomato)
Gene Images
Gene Images
Images are not yet available
Images are not yet available