Standard name
Human Ortholog
Description Putative subunit of the 90S preribosome processome complex; overexpression rescues supressor mutant of mcm10; null mutant is viable; relocalizes from nucleus to cytoplasm upon DNA replication stress
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Micrographs

ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 – – – – – –
Cell Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cytoplasm 0.12 0.2 0.31 0.27 0.79 0.73 0.76 0.72 0.52 0.51 0.44 0.47 0.28 0.59 0.84 0.47 0.27 0.33 0.05 0.09 0.1 0.12 0.17 0.29
Endoplasmic Reticulum 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0.09 0.06 0.08 0 0 0 0 0 0
Endosome 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0.05 0.05 0.05 0 0 0 0 0 0
Golgi 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Mitochondria 0.11 0.16 0.05 0.14 0.13 0.22 0.21 0.19 0.63 0.43 0.59 0.52 0 0 0 0.09 0.07 0 0 0 0 0 0.05 0.05
Nucleus 0.65 0.62 0.52 0.59 0.06 0.08 0.06 0.07 0.1 0.16 0.18 0.18 0.67 0.28 0.05 0.18 0.49 0.44 0.63 0.61 0.51 0.48 0.27 0.15
Nuclear Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0.07
Vac/Vac Membrane 0.24 0.15 0.15 0.1 0.06 0.1 0 0.05 0 0.05 0 0 0.09 0.17 0.14 0.3 0.22 0.22 0.22 0.21 0.24 0.26 0.31 0.32
ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 1 0 0 0 1 0 0 0 0 0 0 0 0 0 1 4 1 0 0 1
Bud 0 0 0 1 2 1 2 4 4 8 4 3 0 0 0 0 1 0 3 5 2 2 4 5
Bud Neck 0 0 1 2 7 7 5 15 2 8 3 3 1 0 0 0 0 0 0 1 1 0 2 3
Bud Site 0 0 0 1 0 1 0 0 9 4 6 4 0 0 0 0 0 0 – – – – – –
Cell Periphery 1 1 0 0 0 0 0 0 0 0 3 0 0 0 0 1 0 1 0 0 0 0 0 0
Cytoplasm 34 102 52 53 168 231 314 247 179 197 173 205 49 110 163 257 98 117 16 52 23 22 39 78
Endoplasmic Reticulum 1 4 2 0 0 0 0 0 0 0 0 1 0 1 0 48 22 28 1 1 1 0 2 2
Endosome 1 0 5 2 0 0 6 2 0 1 0 1 1 3 4 26 18 19 3 7 2 0 7 8
Golgi 1 1 1 0 1 0 0 0 0 0 0 0 0 0 0 2 0 2 2 2 1 0 1 0
Mitochondria 31 85 9 27 27 70 86 64 220 166 235 225 0 1 2 51 24 4 5 12 5 3 10 12
Nucleus 185 323 88 115 13 25 26 23 33 61 73 80 116 52 10 100 180 156 208 374 121 86 61 41
Nuclear Periphery 0 0 0 0 0 0 1 0 0 0 0 2 0 0 0 0 1 0 0 0 0 1 1 1
Nucleolus 0 0 0 0 0 0 1 0 1 1 1 1 0 0 0 1 0 1 0 0 0 3 6 4
Peroxisomes 0 0 0 0 0 1 0 0 0 1 0 0 0 0 2 1 0 0 0 1 1 0 0 0
SpindlePole 0 1 1 5 1 2 8 10 4 10 1 8 1 0 0 4 1 3 12 12 8 5 9 17
Vac/Vac Membrane 67 79 26 20 13 30 14 16 6 18 5 15 15 32 27 161 82 79 70 129 56 47 72 87
Unique Cell Count 283 520 169 196 213 315 412 343 347 386 396 435 174 186 194 542 368 357 329 615 239 179 230 274
Labelled Cell Count 321 596 185 226 233 368 463 381 459 475 504 548 183 199 208 652 427 410 329 615 239 179 230 274


Nucleus

(Huh et al. Nature 2003 425:686)
Screen WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 AF100 AF140 AF180
Mean Cell GFP Intensity (1e-4) 4.7 4.0 5.3 4.9 4.3 4.0 4.1 4.3 3.2 3.7 3.3 3.6 5.2 4.9 4.8 5.0 6.0 6.6 6.2 6.7 6.3
Std Deviation (1e-4) 1.0 0.5 1.3 1.5 1.6 1.0 1.7 1.3 0.9 1.1 0.9 1.1 1.3 1.1 1.4 1.8 2.0 2.2 3.0 4.0 3.1
Intensity Change (Log2) – – – -0.11 -0.3 -0.41 -0.34 -0.29 -0.73 -0.51 -0.66 -0.54 -0.01 -0.12 -0.14 -0.07 0.19 0.33 0.23 0.36 0.26


Localization RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3
Actin – – – – – – – – – – – – – – –
Bud – – – – – – – – – – – – 0 0 0
Bud Neck – – – – – – – – – – – – 0 0 0
Bud Site – – – – – – – – – – – – 0 0 0
Cell Periphery – – – – – – – – – – – – 0 0 0
Cyto – – – – – – – – – – – – – – –
Endoplasmic Reticulum – – – – – – – – – – – – 0 0 0
Endosome – – – – – – – – – – – – 0 0 0
Golgi – – – – – – – – – – – – 0 0 0
Mitochondria – – – – – – – – – – – – 0 0 0
Nuclear Periphery – – – – – – – – – – – – 0 0 0
Nuc – – – – – – – – – – – – – – –
Nucleolus – – – – – – – – – – – – 0 0 0
Peroxisomes – – – – – – – – – – – – 0 0 0
SpindlePole – – – – – – – – – – – – 0 0 0
Vac – – – – – – – – – – – – – – –
Cortical Patches – – – – – – – – – – – – 0 0 0
Cytoplasm – – – – – – – – – – – – 0 0 0
Nucleus – – – – – – – – – – – – 0 0 0
Vacuole – – – – – – – – – – – – 3.7 1.9 1.8

Images

Circle plot not available

Protein Concentration and Protein Localization Data

R1 R2 R3
G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase
Concentration 3.9525 3.4342 3.6964 2.5938 4.0337 3.4563 6.4927 6.7073 6.4822 6.8443 6.1945 6.5071 4.2658 4.1467 4.1655 3.8432 4.268 3.8376
Actin 0.0343 0.0002 0.0207 0.021 0.0506 0.0066 0.0346 0.0001 0.0235 0.0049 0.0108 0.0071 0.0297 0.0002 0.0085 0.0104 0.002 0.0007
Bud 0.0005 0.0001 0.0004 0.0001 0.0007 0.0001 0.0012 0.0001 0.0003 0.0021 0.0002 0.0003 0.0005 0.0001 0.0001 0.0001 0.0009 0.0001
Bud Neck 0.0028 0.0004 0.0007 0.0002 0.0024 0.0023 0.0004 0.0002 0.0052 0.0192 0.0012 0.0013 0.0086 0.0003 0.0011 0.0002 0.0006 0.0018
Bud Periphery 0.0019 0.0002 0.0011 0.0001 0.0014 0.0004 0.0025 0.0001 0.0005 0.0086 0.0001 0.0001 0.0009 0.0003 0.0001 0.0001 0.0011 0.0004
Bud Site 0.0038 0.0011 0.0017 0.0003 0.0126 0.0022 0.0012 0.0004 0.0021 0.0124 0.0007 0.0002 0.0039 0.0002 0.0014 0.0002 0.0014 0.0003
Cell Periphery 0.0024 0.0001 0.0004 0 0.0005 0.0002 0.0002 0 0.0002 0.0019 0.0001 0 0.0002 0 0.0001 0 0.0002 0
Cytoplasm 0.0145 0.0018 0.0052 0.0008 0.0072 0.0047 0.0015 0.0098 0.0026 0.0019 0.0184 0.0061 0.0084 0.0036 0.012 0.0038 0.0258 0.0008
Cytoplasmic Foci 0.0149 0.0007 0.0039 0.0066 0.0251 0.0018 0.0014 0.0002 0.0064 0.0002 0.0043 0.004 0.0202 0.0002 0.0043 0.0025 0.0104 0.0026
Eisosomes 0.0004 0 0.0005 0.0001 0.0013 0.0001 0.0004 0 0.0001 0.0003 0 0 0.0003 0 0.0001 0.0001 0.0001 0
Endoplasmic Reticulum 0.0044 0.0002 0.0035 0.0002 0.0027 0.001 0.0063 0.0007 0.0017 0.0007 0.0067 0.0002 0.0034 0.0024 0.0009 0.0004 0.0102 0.0002
Endosome 0.0179 0.002 0.0093 0.0011 0.0485 0.0077 0.0114 0.0014 0.0033 0.0013 0.0141 0.0012 0.0302 0.0018 0.0057 0.0027 0.0452 0.001
Golgi 0.0065 0.0004 0.0054 0.0017 0.0463 0.003 0.0035 0 0.0027 0.0004 0.0022 0.001 0.0073 0.0001 0.0027 0.0015 0.0033 0.0005
Lipid Particles 0.037 0.0005 0.0042 0.0102 0.0498 0.0035 0.0036 0 0.0007 0.0002 0.0014 0.0067 0.0156 0.0001 0.0043 0.0044 0.0088 0.0005
Mitochondria 0.0143 0.0055 0.014 0.0012 0.0304 0.0025 0.0058 0.0004 0.0009 0.0075 0.0018 0.0005 0.0041 0.0007 0.004 0.0009 0.0076 0.0004
None 0.0159 0.0006 0.0041 0.0005 0.0104 0.0117 0.003 0.0088 0.0015 0.0009 0.003 0.0011 0.002 0.0079 0.0055 0.0099 0.075 0.0003
Nuclear Periphery 0.0365 0.0096 0.0104 0.0029 0.0178 0.0022 0.0261 0.0117 0.0127 0.0105 0.0179 0.0019 0.0436 0.008 0.0089 0.0044 0.0222 0.0015
Nucleolus 0.0132 0.0291 0.0157 0.0122 0.0146 0.032 0.0055 0.0123 0.0153 0.0289 0.013 0.0293 0.0185 0.0126 0.012 0.0149 0.0085 0.0338
Nucleus 0.7399 0.9424 0.8803 0.9294 0.6265 0.9115 0.8471 0.9476 0.8866 0.8913 0.8859 0.9302 0.7672 0.9569 0.9163 0.9355 0.7547 0.9493
Peroxisomes 0.0117 0.0007 0.0074 0.0098 0.0125 0.0008 0.0008 0 0.012 0.0003 0.0007 0.0027 0.0137 0 0.0057 0.0045 0.001 0.0011
Punctate Nuclear 0.0139 0.0028 0.0087 0.0012 0.0306 0.0022 0.0387 0.0027 0.0195 0.0019 0.016 0.0053 0.015 0.0027 0.0056 0.0034 0.0079 0.0042
Vacuole 0.0098 0.0013 0.0015 0.0002 0.0033 0.003 0.0016 0.003 0.0017 0.0037 0.0011 0.0005 0.0041 0.0013 0.0005 0.0002 0.0077 0.0005
Vacuole Periphery 0.0034 0.0004 0.0008 0.0001 0.0048 0.0006 0.0033 0.0005 0.0006 0.0008 0.0005 0.0002 0.0027 0.0005 0.0003 0.0002 0.0055 0.0001

Sequencing Data

R1 R2
G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Post-START S/G2 Metaphase Anaphase Telophase
Gene Expression 55.4221 39.41 49.2969 36.6084 31.0293 39.0258 42.6937 34.9626 30.4839 36.2957
Translational Efficiency 1.5047 2.4104 1.564 1.6493 1.985 1.5668 1.4604 1.5818 1.6162 1.6674

Hit Data

Dataset Hit
Protein Concentration ✘
Protein Localization ✘
Gene Expression ✔
Translational Efficiency ✘

Micrographs

Cell Count

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
119 680 2140 1494 1794 1757 2872 1558 1913 2437 5012 3052

Protein Abundance

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
Mean 581.28 778.64 818.01 877.32 677.20 831.54 870.73 885.59 671.23 816.78 848.22 881.54
Standard Deviation 52.16 108.35 102.74 136.58 86.15 103.41 116.57 124.83 87.56 107.46 113.90 130.78
Intensity Change Log 2 — 0.421723 0.492885 0.593870 — 0.296204 0.362643 0.387057 — 0.355540 0.424265 0.486277

Localization Score (DeepLoc)

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
Actin 0.000081 0.000397 0.000301 0.000625 0.000019 0.000328 0.000258 0.000522 0.000023 0.000347 0.000277 0.000573
Bud Neck 0.015035 0.009824 0.010981 0.024941 0.002018 0.006829 0.015800 0.016319 0.002828 0.007664 0.013742 0.020540
Bud Site 0.002374 0.003147 0.003731 0.008906 0.000625 0.004858 0.001096 0.003121 0.000734 0.004381 0.002221 0.005953
Cell Periphery 0.000295 0.000224 0.000102 0.000223 0.000093 0.000254 0.000125 0.000187 0.000105 0.000246 0.000115 0.000205
Cytoplasm 0.300103* 0.078480 0.014420 0.025212 0.116034* 0.064295 0.003485 0.016755 0.127484* 0.068253 0.008154 0.020895
Cytoplasmic Foci 0.217706* 0.018403 0.000430 0.003368 0.008116 0.008716 0.000083 0.000998 0.021154 0.011419 0.000231 0.002158
Eisosomes 0.000157 0.000036 0.000018 0.000024 0.000013 0.000027 0.000021 0.000021 0.000022 0.000030 0.000020 0.000022
Endoplasmic Reticulum 0.000443 0.006921 0.004393 0.006320 0.000899 0.002882 0.005429 0.006831 0.000871 0.004009 0.004987 0.006581
Endosome 0.002618 0.002287 0.001442 0.003709 0.000401 0.002459 0.000375 0.001475 0.000539 0.002411 0.000831 0.002569
Golgi 0.001265 0.000968 0.000180 0.001569 0.000382 0.000559 0.000031 0.000718 0.000437 0.000673 0.000095 0.001135
Lipid Particles 0.022030 0.002851 0.000221 0.000310 0.000886 0.002326 0.000056 0.000254 0.002201 0.002472 0.000126 0.000281
Mitochondria 0.021573 0.003161 0.001011 0.003910 0.001962 0.006439 0.000814 0.001400 0.003182 0.005524 0.000898 0.002628
Mitotic Spindle 0.000180 0.008457 0.002341 0.031794 0.000808 0.005252 0.001727 0.006238 0.000769 0.006146 0.001989 0.018748
None 0.014309 0.005046 0.004688 0.006235 0.005616 0.011401 0.002175 0.005980 0.006156 0.009627 0.003248 0.006105
Nuclear Periphery 0.000950 0.002190 0.000936 0.003478 0.001784 0.001971 0.000991 0.001079 0.001732 0.002032 0.000968 0.002253
Nuclear Periphery Foci 0.000252 0.001713 0.000132 0.001413 0.000073 0.000314 0.000089 0.000197 0.000084 0.000704 0.000107 0.000793
Nucleolus 0.004057 0.003945 0.002015 0.003523 0.001408 0.005048 0.001514 0.001816 0.001572 0.004740 0.001728 0.002651
Nucleus 0.373588* 0.839960* 0.948016* 0.856419* 0.856133* 0.863954* 0.964183* 0.930943* 0.826116* 0.857259* 0.957280* 0.894462*
Peroxisomes 0.014060 0.003461 0.000163 0.000465 0.000335 0.002779 0.000037 0.000126 0.001189 0.002970 0.000091 0.000292
Vacuole 0.008038 0.008030 0.004218 0.014241 0.002051 0.008365 0.001568 0.004266 0.002423 0.008272 0.002700 0.009149
Vacuole Periphery 0.000889 0.000500 0.000261 0.003313 0.000344 0.000944 0.000144 0.000754 0.000378 0.000820 0.000194 0.002006

Localization Changes (T-score)

R1 R2 R1 & R2
UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14 UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14 UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14
Actin -4.83 -4.97 -13.05 -6.13 -7.59 -3.47 -19.52 -10.35 -2.39 -5.21 -4.96 -14.83 -19.88 -4.66 -9.46
Bud Neck 0.72 0.42 -2.15 -7.79 -8.22 -4.75 -15.83 -13.37 -8.18 -1.49 -5.08 -14.80 -17.88 -12.09 -6.96
Bud Site -0.95 -1.33 -4.85 -4.56 -3.86 -4.08 -2.11 -4.87 1.16 -3.97 -4.75 -4.22 -8.63 -1.59 -5.62
Cell Periphery 0.89 3.47 1.53 0.32 -4.99 -4.29 -1.32 -2.83 2.27 -1.68 -4.41 -0.29 -4.58 1.91 -4.25
Cytoplasm 9.77 13.08 12.43 8.09 -5.92 9.43 26.07 21.53 12.52 -8.33 11.21 27.14 23.11 14.25 -10.34
Cytoplasmic Foci 9.90 10.92 10.79 6.06 -4.83 -0.59 9.46 8.16 8.78 -1.90 4.48 11.24 10.24 9.81 -5.17
Eisosomes 8.72 10.48 9.26 0.51 -8.42 -8.02 -6.26 -8.19 1.70 -2.32 -4.01 0.88 -4.83 0.34 -7.90
Endoplasmic Reticulum -10.67 -14.34 -20.18 -2.08 -7.43 -5.68 -19.64 -17.04 -8.91 -4.14 -10.00 -21.28 -24.44 -9.60 -8.26
Endosome 0.57 2.02 0.38 -0.19 -1.73 -5.35 0.70 -1.70 2.56 -1.93 -5.98 -0.79 -3.79 1.76 -2.80
Golgi 0.55 2.84 1.03 0.50 -2.41 -0.62 1.44 -0.29 0.15 -1.42 -0.92 1.50 -0.70 0.12 -2.66
Lipid Particles 3.44 3.91 3.88 4.84 -3.24 -5.09 8.31 5.54 7.51 -3.81 -0.70 5.44 4.88 8.80 -5.48
Mitochondria 1.90 2.13 1.85 -0.62 -6.54 -3.72 1.51 0.51 4.78 -5.56 -2.12 2.47 0.60 3.78 -7.98
Mitotic Spindle -2.87 -3.26 -8.63 -4.80 -7.75 -3.24 -1.45 -3.86 -1.08 -3.20 -4.11 -2.21 -9.05 -5.39 -8.32
None 1.81 1.96 1.77 -0.34 -1.40 -3.46 3.84 0.32 3.76 -3.73 -2.48 3.42 0.87 3.67 -4.03
Nuclear Periphery -2.95 -0.49 -7.01 -5.47 -8.65 -0.49 1.46 0.66 1.54 -2.29 -0.81 1.54 -3.42 -3.41 -8.79
Nuclear Periphery Foci -1.55 0.71 -5.47 0.07 -5.88 -4.44 -0.42 -3.69 1.53 -3.07 -2.36 -1.65 -6.76 -0.71 -6.37
Nucleolus 0.04 1.62 -1.42 -2.16 -4.93 -5.00 -0.11 -1.21 4.22 -1.40 -5.41 -0.44 -4.58 1.23 -5.13
Nucleus -16.32 -21.24 -16.87 -0.02 15.04 -0.77 -19.42 -10.12 -8.33 9.22 -3.83 -21.54 -7.89 -3.96 17.72
Peroxisomes 2.55 3.44 3.40 2.92 -1.63 -5.28 5.52 3.69 5.79 -3.13 -3.39 4.09 3.61 6.15 -2.71
Vacuole -0.51 2.00 -7.57 -7.57 -9.46 -5.84 0.05 -6.87 0.95 -7.04 -7.12 -2.41 -12.30 -5.75 -11.38
Vacuole Periphery 1.13 1.85 -1.46 -2.59 -3.11 -2.08 1.18 -0.71 1.11 -1.64 -1.90 1.12 -2.42 -0.95 -3.55
Standard name
Human Ortholog
Description Putative subunit of the 90S preribosome processome complex; overexpression rescues supressor mutant of mcm10; null mutant is viable; relocalizes from nucleus to cytoplasm upon DNA replication stress
Localization
Cell Percentages nucleus (84%)
Cell Cycle Regulation No
Subcompartmental Group nucleus-3

Cms1

Cms1


Nearest neighbours ( genes)

correlation threshold

0.6

1.0

0.80


Biological Process (Gene Ontology)
Molecular Function (Gene Ontology)
Cellular Component (Gene Ontology)

Endocytosis
Temp Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Vph1-GFP)
37℃
RT
Cell Cycle Omics

CYCLoPs (Cms1-GFP)
Gene / Allele Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Sac6-tdTomato)
Gene Images
Gene Images
Images are not yet available
Images are not yet available