Standard name
Human Ortholog
Description Subunit of the core form of RNA polymerase transcription factor TFIIH; has both protein kinase and DNA-dependent ATPase/helicase activities; essential for transcription and nucleotide excision repair; interacts with Tfb4p
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Micrographs

ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0 0.05 0 0 0 0 0 0 0 – – – – – –
Cell Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cytoplasm 0.06 0.08 0 0 0.07 0 0.07 0.07 0 0.05 0 0.05 0.14 0.12 0.17 0.09 0.05 0 0 0 0 0 0 0
Endoplasmic Reticulum 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Endosome 0 0.05 0 0 0 0 0 0 0 0 0 0 0.05 0 0 0 0 0 0 0 0 0 0 0
Golgi 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Mitochondria 0.44 0.3 0.59 0.48 0.45 0.68 0.71 0.64 0.66 0.74 0.71 0.73 0.06 0.06 0.05 0.1 0.08 0.18 0 0 0 0 0 0
Nucleus 0.62 0.54 0.8 0.81 0.78 0.68 0.61 0.66 0.66 0.6 0.72 0.64 0.73 0.77 0.67 0.83 0.82 0.87 0.49 0.58 0.63 0.66 0.62 0.47
Nuclear Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0.24 0.15 0 0 0 0 0.1 0.06 0.08 0.08 0.12 0.11 0.08 0.1 0.09 0 0.05 0 0.18 0.1 0.19 0.16 0.19 0.23
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0.07 0.05 0 0 0.05 0.07
Vac/Vac Membrane 0.06 0.16 0 0 0 0 0 0 0 0 0 0 0.07 0 0.07 0.05 0 0 0.1 0.13 0.07 0.06 0.06 0.11
ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 0 0 0 0 0 1 0 0 0 0 0 0 1 0 0 0 0 0
Bud 1 0 1 0 1 1 4 2 1 2 1 5 0 0 0 0 0 0 7 3 1 2 3 6
Bud Neck 0 0 1 0 0 0 0 3 0 0 0 0 0 0 0 0 0 0 2 0 0 0 1 2
Bud Site 1 0 0 0 0 5 10 4 10 14 17 7 1 0 0 0 0 0 – – – – – –
Cell Periphery 0 0 0 0 0 0 0 0 1 0 0 0 0 0 0 0 1 0 0 0 0 0 0 0
Cytoplasm 18 11 2 6 13 12 27 25 15 19 6 15 34 54 76 18 5 3 9 6 1 3 3 2
Endoplasmic Reticulum 0 0 1 0 0 1 0 0 1 0 0 0 0 0 0 5 3 2 0 0 0 1 2 3
Endosome 4 7 0 0 2 0 1 0 0 0 0 0 12 9 16 0 4 1 13 6 1 2 2 8
Golgi 0 0 0 0 0 0 0 0 0 0 0 0 0 0 1 0 0 0 1 0 0 0 1 1
Mitochondria 128 43 75 108 83 204 267 241 248 282 241 218 14 25 21 20 9 12 9 2 2 1 1 7
Nucleus 183 78 101 181 142 203 229 251 247 230 245 192 181 349 296 169 91 59 169 96 84 140 154 147
Nuclear Periphery 1 1 1 2 0 1 2 4 9 11 4 9 1 2 0 3 1 0 2 1 0 1 2 1
Nucleolus 71 22 4 8 3 11 38 23 31 32 40 33 21 46 39 4 5 0 60 17 25 34 47 73
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 1 5 1 0 0 0 0 0 0 0 0
SpindlePole 4 1 3 3 2 3 2 5 1 6 2 1 2 2 9 3 1 1 24 8 2 8 12 21
Vac/Vac Membrane 17 23 0 5 3 4 5 4 3 3 1 3 17 17 32 10 4 3 34 21 9 13 14 35
Unique Cell Count 294 145 127 224 183 299 378 379 376 382 341 298 249 453 440 204 111 68 345 166 134 214 249 317
Labelled Cell Count 428 186 189 313 249 445 585 562 567 599 557 484 283 505 495 233 124 81 345 166 134 214 249 317


Nucleus

(Huh et al. Nature 2003 425:686)
Screen WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 AF100 AF140 AF180
Mean Cell GFP Intensity (1e-4) 4.3 4.7 3.9 4.1 4.1 3.6 3.4 3.6 3.6 3.4 3.4 3.4 5.7 5.7 5.8 5.6 6.9 7.0 5.3 5.6 6.2
Std Deviation (1e-4) 0.8 1.2 0.6 0.8 1.0 0.9 1.2 1.1 0.8 1.2 0.8 1.1 1.6 1.6 1.8 1.6 1.9 1.8 1.5 2.0 1.9
Intensity Change (Log2) – – – 0.05 0.07 -0.13 -0.23 -0.11 -0.14 -0.2 -0.22 -0.23 0.54 0.54 0.56 0.52 0.8 0.83 0.43 0.52 0.66


Localization RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3
Actin – – – – – – – – – – – – – – –
Bud – – – – – – – – – – – – – – –
Bud Neck – – – – – – – – – – – – – – –
Bud Site – – – – – – – – – – – – – – –
Cell Periphery – – – – – – – – – – – – – – –
Cyto – – – – – – – – – – – – – – –
Endoplasmic Reticulum – – – – – – – – – – – – – – –
Endosome – – – – – – – – – – – – – – –
Golgi – – – – – – – – – – – – – – –
Mitochondria – – – – – – – – – – – – – – –
Nuclear Periphery – – – – – – – – – – – – – – –
Nuc – – – – – – – – – – – – – – –
Nucleolus – – – – – – – – – – – – – – –
Peroxisomes – – – – – – – – – – – – – – –
SpindlePole – – – – – – – – – – – – – – –
Vac – – – – – – – – – – – – – – –

Images

Circle plot not available

Protein Concentration and Protein Localization Data

R1 R2 R3
G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase
Concentration 2.9724 2.9543 3.0155 2.5513 2.2862 2.732 1.8917 1.7496 1.6834 1.0494 1.5022 1.72 3.499 3.4668 3.2719 3.6587 3.466 3.2296
Actin 0.0095 0 0.002 0.0001 0.0002 0.0027 0.0152 0.0174 0.003 0 0.0089 0.0228 0.0301 0.0004 0.0045 0.0001 0.0171 0.0049
Bud 0.0006 0.0001 0.0004 0.0002 0.0002 0.0002 0.0006 0.0002 0.0001 0 0.0014 0.0004 0.0004 0.0003 0.0004 0.0001 0.0002 0.0001
Bud Neck 0.0022 0.0005 0.0007 0.0002 0.0009 0.0014 0.0016 0.0003 0.0002 0 0.0004 0.0015 0.0028 0.0004 0.004 0.0004 0.0018 0.0016
Bud Periphery 0.0011 0.0001 0.0009 0.0005 0.0006 0.0003 0.0013 0.0002 0.0001 0 0.0013 0.0009 0.001 0.0006 0.0011 0.0002 0.0004 0.0002
Bud Site 0.0038 0.0007 0.0057 0.0016 0.0007 0.0003 0.002 0.0004 0.0004 0 0.0044 0.001 0.0036 0.0009 0.0025 0.0003 0.0005 0.0002
Cell Periphery 0.0003 0 0.0004 0.0001 0.0001 0 0.0006 0 0 0 0.0001 0.0001 0.0004 0.0001 0.0003 0.0001 0.0001 0
Cytoplasm 0.0117 0.0002 0.0018 0.0001 0.0001 0.0021 0.0098 0.0029 0.0014 0 0.0021 0.0002 0.0022 0.0023 0.0075 0.0014 0.0029 0.0004
Cytoplasmic Foci 0.0121 0.0036 0.0013 0.0058 0.0001 0.0008 0.0123 0.001 0.0066 0 0.0322 0.0009 0.0077 0.0003 0.0037 0.0007 0.0069 0.001
Eisosomes 0.0003 0 0.0001 0 0 0.0001 0.0004 0 0 0 0.0001 0 0.0003 0 0.0001 0 0.0002 0
Endoplasmic Reticulum 0.0057 0 0.0006 0 0 0.001 0.0123 0.0007 0.0003 0 0.0009 0.0001 0.0019 0.0007 0.0026 0.0009 0.0008 0.0003
Endosome 0.0247 0.0005 0.0024 0.0035 0.0002 0.0039 0.0402 0.0026 0.0125 0 0.0533 0.0023 0.0106 0.0017 0.0042 0.0046 0.0351 0.0031
Golgi 0.006 0.0002 0.0005 0.002 0.0001 0.0008 0.0346 0.0014 0.0188 0 0.027 0.0028 0.008 0.0003 0.0017 0.0002 0.0043 0.0026
Lipid Particles 0.0277 0.0008 0.0013 0.022 0.0003 0.0015 0.035 0.0004 0.0024 0 0.0195 0.0021 0.0098 0.0003 0.0012 0.0012 0.0153 0.0013
Mitochondria 0.0119 0.0002 0.0111 0.0079 0.0007 0.0071 0.0438 0.0054 0.0108 0 0.0102 0.0073 0.0048 0.0003 0.0017 0.0003 0.006 0.0014
None 0.0099 0 0.0006 0.0001 0.0001 0.0041 0.0121 0.0003 0.0014 0 0.0013 0.0003 0.0032 0.0003 0.0071 0.0004 0.0007 0.001
Nuclear Periphery 0.0214 0.0006 0.0045 0.0013 0.0091 0.0071 0.0478 0.0033 0.0019 0.0003 0.0015 0.0033 0.0114 0.0025 0.0039 0.0037 0.004 0.0049
Nucleolus 0.0414 0.0313 0.0259 0.0167 0.1353 0.0665 0.0436 0.0255 0.0108 0.0102 0.056 0.0467 0.0347 0.0274 0.0165 0.0132 0.0932 0.0614
Nucleus 0.7686 0.9564 0.9272 0.911 0.827 0.8804 0.6547 0.9332 0.8994 0.9893 0.7266 0.8879 0.8458 0.9484 0.9279 0.964 0.7921 0.9113
Peroxisomes 0.0066 0.0003 0.0012 0.0246 0.0001 0.0004 0.0035 0.0011 0.0013 0 0.0306 0.0012 0.009 0 0.0013 0.0001 0.0043 0.0003
Punctate Nuclear 0.0302 0.0042 0.0091 0.0008 0.024 0.0186 0.0145 0.0032 0.0276 0.0001 0.0186 0.017 0.0067 0.0111 0.0053 0.006 0.0109 0.0033
Vacuole 0.0027 0.0002 0.0013 0.0013 0.0002 0.0005 0.0092 0.0002 0.0004 0 0.0024 0.0009 0.0036 0.0015 0.0019 0.0017 0.0022 0.0004
Vacuole Periphery 0.0017 0 0.0007 0.0003 0.0001 0.0003 0.0049 0.0002 0.0005 0 0.0012 0.0004 0.0017 0.0003 0.0005 0.0003 0.001 0.0002

Sequencing Data

R1 R2
G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Post-START S/G2 Metaphase Anaphase Telophase
Gene Expression 31.0482 21.3501 16.3708 20.2411 19.0955 16.709 21.5871 25.0116 23.1963 25.1834
Translational Efficiency 0.9179 1.1812 1.2202 1.234 1.2486 1.8016 1.15 0.87 1.0099 0.9838

Hit Data

Dataset Hit
Protein Concentration ✘
Protein Localization ✘
Gene Expression ✘
Translational Efficiency ✘

Micrographs

Cell Count

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
606 1115 1756 1122 317 1908 111 736 923 3023 1867 1858

Protein Abundance

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
Mean 724.38 763.63 871.40 959.50 808.66 780.55 1128.51 940.47 753.33 774.31 886.69 951.96
Standard Deviation 115.70 135.03 155.74 139.83 122.17 139.06 162.94 141.69 124.57 137.83 167.60 140.88
Intensity Change Log 2 — 0.076127 0.266588 0.405536 — -0.051042 0.480814 0.217849 — 0.010446 0.383540 0.309581

Localization Score (DeepLoc)

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
Actin 0.000409 0.001053 0.000857 0.001870 0.000540 0.000617 0.002143 0.002259 0.000454 0.000778 0.000934 0.002024
Bud Neck 0.011411 0.010140 0.023973 0.037474 0.006923 0.012099 0.014014 0.027328 0.009870 0.011376 0.023381 0.033455
Bud Site 0.001562 0.001854 0.001942 0.007370 0.000775 0.002446 0.001762 0.010938 0.001292 0.002227 0.001931 0.008784
Cell Periphery 0.000119 0.000155 0.000112 0.000219 0.000062 0.000119 0.000078 0.000324 0.000100 0.000132 0.000110 0.000260
Cytoplasm 0.016990 0.009161 0.002994 0.007263 0.004529 0.007247 0.001745 0.020897 0.012710 0.007953 0.002919 0.012663
Cytoplasmic Foci 0.001908 0.001887 0.000122 0.000593 0.001047 0.001685 0.000169 0.006566 0.001612 0.001760 0.000125 0.002959
Eisosomes 0.000028 0.000033 0.000035 0.000047 0.000029 0.000030 0.000064 0.000043 0.000028 0.000031 0.000036 0.000045
Endoplasmic Reticulum 0.003649 0.005370 0.002944 0.007008 0.002767 0.003641 0.006519 0.006494 0.003346 0.004279 0.003156 0.006804
Endosome 0.001299 0.001321 0.000205 0.001277 0.000507 0.002632 0.000264 0.005288 0.001027 0.002148 0.000208 0.002866
Golgi 0.000130 0.000416 0.000131 0.001917 0.000116 0.000959 0.000186 0.004256 0.000125 0.000759 0.000134 0.002844
Lipid Particles 0.001492 0.001745 0.000070 0.000277 0.000937 0.001568 0.000376 0.000964 0.001301 0.001633 0.000088 0.000549
Mitochondria 0.001787 0.004431 0.001535 0.004480 0.000838 0.004780 0.001780 0.006340 0.001461 0.004651 0.001549 0.005217
Mitotic Spindle 0.005843 0.005624 0.002221 0.015849 0.001175 0.009875 0.006692 0.054436 0.004240 0.008307 0.002487 0.031134
None 0.007143 0.011083 0.004436 0.004707 0.004542 0.010876 0.002636 0.013139 0.006249 0.010953 0.004329 0.008047
Nuclear Periphery 0.002246 0.001125 0.000402 0.001316 0.000528 0.001232 0.000435 0.001903 0.001656 0.001193 0.000404 0.001549
Nuclear Periphery Foci 0.000741 0.001211 0.000227 0.000639 0.000494 0.001103 0.000270 0.002553 0.000656 0.001143 0.000230 0.001397
Nucleolus 0.049571 0.054906 0.035131 0.019823 0.041327 0.052248 0.025973 0.022076 0.046739 0.053228 0.034586 0.020715
Nucleus 0.889341* 0.884813* 0.921401* 0.880862* 0.931563* 0.882486* 0.931061* 0.800471* 0.903842* 0.883344* 0.921975* 0.849017*
Peroxisomes 0.000598 0.001702 0.000453 0.000498 0.000496 0.000882 0.001295 0.004231 0.000563 0.001184 0.000503 0.001977
Vacuole 0.003134 0.001418 0.000629 0.005874 0.000604 0.002328 0.002297 0.008155 0.002265 0.001992 0.000729 0.006777
Vacuole Periphery 0.000601 0.000553 0.000181 0.000635 0.000203 0.001146 0.000242 0.001339 0.000464 0.000928 0.000184 0.000914

Localization Changes (T-score)

R1 R2 R1 & R2
UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14 UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14 UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14
Actin -5.71 -6.72 -16.38 -10.00 -12.93 -0.88 -8.86 -9.81 -8.82 0.54 -3.57 -9.38 -17.89 -13.77 -12.50
Bud Neck 2.38 -8.59 -11.42 -14.40 -5.84 -3.93 -4.19 -11.60 -9.43 -4.20 -0.09 -11.75 -15.22 -16.58 -5.78
Bud Site 0.50 -0.65 -6.94 -7.38 -6.64 -2.98 -4.57 -7.89 -6.31 -5.83 -1.28 -1.95 -10.30 -9.13 -8.80
Cell Periphery -1.06 0.65 -1.58 -0.82 -2.85 -3.39 -2.40 -6.74 -4.80 -5.23 -1.47 -0.03 -4.43 -4.07 -4.99
Cytoplasm 2.89 5.33 3.70 0.96 -4.13 -0.67 1.76 -3.79 -4.07 -6.35 2.85 5.45 1.32 -2.42 -7.21
Cytoplasmic Foci 0.62 3.59 3.22 3.06 -1.48 0.69 1.81 -0.35 -1.62 -3.43 1.03 3.72 1.35 0.58 -3.67
Eisosomes -0.50 -1.80 -7.60 -8.39 -8.38 0.40 -5.40 -5.89 -9.41 2.16 0.13 -3.01 -9.66 -13.28 -9.09
Endoplasmic Reticulum -3.16 1.99 -7.78 -5.69 -11.49 -3.03 -5.50 -10.03 -8.68 -0.69 -2.63 0.26 -11.66 -11.06 -13.86
Endosome 0.36 5.09 1.84 0.94 -4.26 -3.32 1.24 -2.85 0.14 -3.48 -2.16 5.50 -0.96 1.30 -5.24
Golgi -3.28 0.26 -1.44 -0.90 -1.45 -3.01 -3.86 -2.38 -1.08 -2.17 -3.47 -0.87 -2.65 -1.06 -2.61
Lipid Particles -0.31 2.97 2.37 2.90 -8.28 -0.69 1.08 0.49 1.72 -1.57 -0.47 3.41 2.23 3.41 -6.31
Mitochondria -2.05 0.61 -6.78 0.03 -7.57 -4.94 -7.21 -6.77 0.01 -4.14 -4.57 -1.61 -9.32 0.16 -8.52
Mitotic Spindle 1.34 2.30 -3.74 -5.38 -6.65 -4.79 -1.58 -8.25 -6.61 -3.88 -0.80 1.25 -8.27 -8.17 -9.76
None -2.16 2.84 2.91 5.59 -0.09 -3.99 1.33 -3.06 1.13 -4.16 -3.66 2.76 0.49 4.74 -3.16
Nuclear Periphery 3.54 6.54 0.65 -4.17 -11.17 -2.93 -1.15 -8.58 -3.82 -4.90 2.28 6.24 -2.00 -5.42 -14.44
Nuclear Periphery Foci -0.58 3.07 0.85 1.67 -2.93 -0.54 0.71 -1.49 -1.22 -2.61 -0.54 3.30 -0.46 0.07 -4.49
Nucleolus -1.03 2.62 6.81 10.17 6.57 -2.45 1.65 3.64 9.32 0.86 -1.99 2.52 7.67 14.17 6.81
Nucleus -0.83 -4.14 1.66 3.15 8.27 5.36 1.31 10.08 7.03 6.55 1.69 -2.66 7.09 7.03 12.51
Peroxisomes -2.17 1.53 1.54 3.00 -0.28 -2.13 -3.54 -1.26 -0.95 -0.51 -2.45 1.23 -0.81 0.35 -1.25
Vacuole 2.82 3.93 -4.40 -7.26 -8.16 -1.01 -1.92 -5.53 -5.37 -3.23 1.46 3.35 -6.88 -8.50 -9.84
Vacuole Periphery 0.52 5.37 1.48 0.93 -3.16 -4.50 -1.61 -3.73 1.71 -3.18 -3.13 5.12 -0.90 2.43 -4.93
Standard name
Human Ortholog
Description Subunit of the core form of RNA polymerase transcription factor TFIIH; has both protein kinase and DNA-dependent ATPase/helicase activities; essential for transcription and nucleotide excision repair; interacts with Tfb4p
Localization
Cell Percentages nucleus (99%)
Cell Cycle Regulation No
Subcompartmental Group nucleus-4

Ssl1

Ssl1


Nearest neighbours ( genes)

correlation threshold

0.6

1.0

0.80


Biological Process (Gene Ontology)
Molecular Function (Gene Ontology)
Cellular Component (Gene Ontology)

Endocytosis
Temp Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Vph1-GFP)
37℃
RT
Cell Cycle Omics

CYCLoPs (Ssl1-GFP)
Gene / Allele Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Sac6-tdTomato)
Gene Images
Gene Images
Images are not yet available
Images are not yet available