Standard name
Human Ortholog
Description FMN-dependent NAD(P)H:quinone reductase; role in apoptosis-like cell death; may be involved in quinone detoxification; expression elevated at low temperature; sequesters the Cin5p transcription factor in the cytoplasm in complex with the proteasome under reducing conditions
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Micrographs

ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 – 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud 0 0 – 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 – 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 – 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 – – – – – –
Cell Periphery 0 0 – 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cytoplasm 0.97 0.99 – 0.97 0.93 0.89 0.81 – 0.73 0.76 0.74 0.73 0.99 0.99 0.99 0.95 0.93 0.92 0.95 0.95 0.91 0.94 0.94 0.92
Endoplasmic Reticulum 0 0 – 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Endosome 0 0 – 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Golgi 0 0 – 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Mitochondria 0 0 – 0 0 0.11 0.05 – 0.17 0.1 0.16 0.15 0 0 0 0 0 0 0 0 0 0 0 0
Nucleus 0 0 – 0 0 0 0 – 0 0 0.07 0 0 0 0 0 0 0 0 0 0 0 0 0
Nuclear Periphery 0 0 – 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 – 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 – 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 – 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Vac/Vac Membrane 0 0 – 0 0.11 0.06 0.19 – 0.21 0.18 0.08 0.17 0 0 0 0 0.07 0.07 0 0 0 0 0 0
ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 – 0 0 0 0 – 0 0 1 0 0 0 0 0 0 0 1 0 0 1 2 2
Bud 0 0 – 0 0 2 0 – 4 3 1 1 0 0 0 0 0 0 0 0 0 0 4 1
Bud Neck 0 0 – 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 4
Bud Site 0 0 – 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 – – – – – –
Cell Periphery 0 0 – 2 0 0 4 – 6 2 2 8 0 2 0 2 5 3 0 0 0 0 0 0
Cytoplasm 149 73 – 275 339 187 422 – 268 386 262 377 392 520 629 302 169 173 144 81 27 281 551 412
Endoplasmic Reticulum 2 1 – 0 0 1 0 – 1 0 6 4 3 1 1 12 4 2 0 0 0 2 4 2
Endosome 0 0 – 0 1 0 1 – 0 1 1 0 0 0 1 1 1 0 1 0 0 1 3 4
Golgi 0 0 – 0 0 0 0 – 0 0 0 0 0 2 0 2 2 0 0 0 0 1 3 2
Mitochondria 0 0 – 1 1 23 24 – 62 53 55 78 0 0 0 4 0 3 0 0 0 1 2 3
Nucleus 3 0 – 0 2 4 10 – 13 20 26 19 0 0 0 9 8 6 0 1 0 0 1 2
Nuclear Periphery 0 0 – 0 0 0 0 – 1 1 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 – 0 0 0 0 – 0 0 1 0 0 0 0 0 1 0 0 0 0 0 0 0
Peroxisomes 0 0 – 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 – 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 2 3
Vac/Vac Membrane 1 0 – 9 39 13 99 – 76 91 30 91 5 6 14 13 12 14 0 0 0 4 3 2
Unique Cell Count 153 74 283 363 211 518 367 505 354 520 396 527 635 318 181 188 152 86 30 299 585 450
Labelled Cell Count 155 74 287 382 230 560 431 557 385 578 400 531 645 345 202 201 152 86 30 299 585 450


Cytoplasm, Nucleus

(Huh et al. Nature 2003 425:686)
Screen WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 AF100 AF140 AF180
Mean Cell GFP Intensity (1e-4) 6.8 6.4 – 6.7 7.8 5.3 7.1 – 6.3 7.0 6.3 6.7 7.7 8.2 8.8 8.9 10.1 9.1 7.0 7.2 7.6
Std Deviation (1e-4) 1.0 1.0 – 1.0 0.9 1.1 1.1 – 1.2 1.2 1.1 1.1 1.1 1.4 1.3 1.5 2.1 1.6 1.5 1.4 1.7
Intensity Change (Log2) – – – – – – – – – – – – – – – – – – – – –


Localization RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3
Actin – – – – – – – – – – – – – – –
Bud – – – – – – – – – – – – – – –
Bud Neck – – – – – – – – – – – – – – –
Bud Site – – – – – – – – – – – – – – –
Cell Periphery – – – – – – – – – – – – – – –
Cyto – – – – – – – – – – – – – – –
Endoplasmic Reticulum – – – – – – – – – – – – – – –
Endosome – – – – – – – – – – – – – – –
Golgi – – – – – – – – – – – – – – –
Mitochondria – – – – – – – – – – – – – – –
Nuclear Periphery – – – – – – – – – – – – – – –
Nuc – – – – – – – – – – – – – – –
Nucleolus – – – – – – – – – – – – – – –
Peroxisomes – – – – – – – – – – – – – – –
SpindlePole – – – – – – – – – – – – – – –
Vac – – – – – – – – – – – – – – –
Cortical Patches – – – – – – – – – – – – – – –
Cytoplasm – – – – – – – – – – – – – – –
Nucleus – – – – – – – – – – – – – – –
Vacuole – – – – – – – – – – – – – – –

Images

Circle plot not available

Protein Concentration and Protein Localization Data

R1 R2 R3
G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase
Concentration 1.9358 2.8038 2.4489 2.4368 1.9751 2.4243 3.2952 5.0836 4.3344 4.164 3.6157 4.1569 4.5293 6.8214 5.6436 5.8765 5.0095 5.6133
Actin 0.0067 0.0001 0.0019 0.0052 0.0127 0.0031 0.0059 0.0004 0.0006 0.0018 0.0003 0.0003 0.0027 0.0003 0.0044 0.002 0.0001 0.0003
Bud 0.0063 0.0001 0.0148 0.0459 0.0047 0.0119 0.0037 0.0001 0.0003 0.0013 0.0003 0.0001 0.0001 0.0001 0.0001 0.0002 0.0001 0
Bud Neck 0.0009 0.0001 0.0006 0.0009 0.0009 0.0017 0.0002 0.0002 0.0003 0.0003 0.0004 0.0008 0.0002 0.0001 0.0003 0.0005 0.0005 0.0008
Bud Periphery 0.0031 0 0.0025 0.0129 0.0019 0.0038 0.001 0 0.0001 0.0002 0 0 0 0 0.0001 0.0001 0 0
Bud Site 0.0139 0.0003 0.0158 0.004 0.0041 0.0006 0.0011 0.0003 0.0015 0.0006 0.0001 0.0001 0.0001 0.0003 0.0006 0.0002 0 0.0001
Cell Periphery 0.0004 0 0.0005 0.0003 0.0002 0.0002 0.0002 0 0 0.0002 0 0 0.0001 0.0001 0.0001 0.0001 0 0
Cytoplasm 0.7902 0.947 0.8233 0.7415 0.876 0.8466 0.8938 0.97 0.9522 0.8743 0.9024 0.9501 0.8962 0.9544 0.9151 0.9386 0.9754 0.9505
Cytoplasmic Foci 0.0258 0.0034 0.0152 0.0124 0.0152 0.0178 0.0064 0.0024 0.0036 0.007 0.0137 0.0078 0.0176 0.0016 0.0052 0.006 0.0027 0.003
Eisosomes 0.0001 0 0.0001 0.0001 0 0 0.0001 0 0 0.0001 0 0 0.0001 0 0.0001 0 0 0
Endoplasmic Reticulum 0.005 0.0014 0.0025 0.0074 0.0023 0.0023 0.0035 0.0016 0.0017 0.008 0.0072 0.0016 0.0038 0.0006 0.0019 0.0032 0.0019 0.0019
Endosome 0.0089 0.0016 0.008 0.0049 0.0039 0.0079 0.0059 0.0007 0.0011 0.0035 0.0041 0.0066 0.0039 0.0003 0.0025 0.0015 0.0008 0.0012
Golgi 0.001 0.0001 0.0007 0.0004 0.0005 0.0002 0.0007 0.0001 0.0001 0.0002 0.0001 0.0002 0.0004 0 0.0016 0.0002 0 0.0001
Lipid Particles 0.0009 0 0.0005 0.0006 0.0002 0.0001 0.0003 0 0 0.0006 0.0002 0 0.0002 0 0.0017 0.0001 0 0
Mitochondria 0.0042 0.0001 0.0024 0.0055 0.0014 0.0022 0.0006 0.0001 0.0001 0.0025 0.0002 0.0002 0.0004 0.0001 0.0003 0.0002 0.0001 0.0001
None 0.1057 0.0406 0.0589 0.0535 0.0447 0.0638 0.0616 0.017 0.0307 0.084 0.0451 0.014 0.0615 0.0403 0.0576 0.0396 0.0112 0.0305
Nuclear Periphery 0.0041 0.0007 0.0031 0.0136 0.0031 0.0026 0.0026 0.0009 0.0012 0.0019 0.0034 0.0012 0.0047 0.0003 0.0008 0.0013 0.0008 0.0007
Nucleolus 0.001 0 0.0021 0.0032 0.0005 0.0013 0.0003 0 0 0.0033 0.0002 0 0.0001 0 0.0001 0.0001 0 0
Nucleus 0.0099 0.0035 0.0215 0.0444 0.0189 0.0234 0.0081 0.0048 0.0052 0.007 0.0159 0.0147 0.0058 0.0013 0.0039 0.0036 0.0055 0.01
Peroxisomes 0.0018 0 0.0006 0.001 0.0007 0.0002 0.0002 0.0001 0.0001 0.0001 0.0001 0.0001 0.0003 0 0.0026 0.0001 0 0
Punctate Nuclear 0.008 0.0003 0.0223 0.0395 0.0064 0.0082 0.0024 0.0009 0.0008 0.0016 0.0049 0.0008 0.0013 0.0001 0.0006 0.0019 0.0004 0.0005
Vacuole 0.0019 0.0006 0.0019 0.002 0.0014 0.0017 0.0012 0.0003 0.0004 0.0014 0.0013 0.0013 0.0004 0.0001 0.0003 0.0004 0.0003 0.0002
Vacuole Periphery 0.0004 0.0001 0.0007 0.0009 0.0004 0.0005 0.0003 0 0.0001 0.0003 0.0002 0.0002 0.0002 0 0.0001 0.0001 0.0001 0

Sequencing Data

R1 R2
G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Post-START S/G2 Metaphase Anaphase Telophase
Gene Expression 12.4516 17.1246 22.8729 11.9611 11.1767 7.6583 18.1804 19.8666 18.3003 15.1748
Translational Efficiency 2.0986 1.7043 1.1741 1.9782 1.8663 2.4743 1.5745 1.7522 1.6171 1.4303

Hit Data

Dataset Hit
Protein Concentration ✔
Protein Localization ✘
Gene Expression ✔
Translational Efficiency ✘

Micrographs

Cell Count

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
1244 1434 131 1554 1101 2102 1706 1167 2345 3536 1837 2721

Protein Abundance

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
Mean 833.18 856.97 1108.48 990.07 825.22 908.59 1002.21 1033.99 829.44 887.66 1009.79 1008.91
Standard Deviation 118.87 132.99 130.19 137.48 107.76 119.17 122.99 154.53 113.86 127.51 126.51 146.66
Intensity Change Log 2 — 0.040616 0.411883 0.248902 — 0.138851 0.280334 0.325372 — 0.090334 0.347923 0.287460

Localization Score (DeepLoc)

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
Actin 0.000301 0.000179 0.000993 0.001481 0.000213 0.000208 0.000183 0.000352 0.000260 0.000196 0.000240 0.000997
Bud Neck 0.002410 0.002272 0.002620 0.004307 0.001424 0.004052 0.000949 0.004430 0.001947 0.003330 0.001068 0.004360
Bud Site 0.004975 0.007309 0.007354 0.029662 0.004064 0.009986 0.003715 0.032308 0.004547 0.008900 0.003975 0.030797
Cell Periphery 0.000127 0.000070 0.000354 0.000120 0.000131 0.000071 0.000081 0.000111 0.000128 0.000071 0.000100 0.000116
Cytoplasm 0.661739* 0.761019* 0.493514* 0.616417* 0.746972* 0.734521* 0.700065* 0.677239* 0.701757* 0.745267* 0.685335* 0.642503*
Cytoplasmic Foci 0.151737 0.061538 0.023094 0.018634 0.087185 0.071246 0.011517 0.013198 0.121429 0.067309 0.012342 0.016303
Eisosomes 0.000060 0.000017 0.000064 0.000026 0.000031 0.000026 0.000015 0.000015 0.000047 0.000022 0.000018 0.000021
Endoplasmic Reticulum 0.001360 0.001490 0.007523 0.001722 0.001361 0.000402 0.001355 0.000825 0.001361 0.000843 0.001794 0.001337
Endosome 0.008278 0.003535 0.006475 0.008161 0.005136 0.003957 0.001253 0.005730 0.006803 0.003786 0.001625 0.007119
Golgi 0.001300 0.000949 0.000294 0.004087 0.001145 0.001419 0.000084 0.002692 0.001227 0.001228 0.000099 0.003489
Lipid Particles 0.002505 0.000957 0.002586 0.001405 0.001977 0.000756 0.000562 0.000558 0.002258 0.000837 0.000706 0.001042
Mitochondria 0.003459 0.000648 0.000349 0.001523 0.000859 0.001498 0.000205 0.001085 0.002239 0.001153 0.000216 0.001335
Mitotic Spindle 0.001641 0.001716 0.001972 0.048705 0.000419 0.007292 0.001903 0.016020 0.001067 0.005031 0.001908 0.034687
None 0.002125 0.008206 0.001716 0.006167 0.005246 0.004936 0.007220 0.010484 0.003591 0.006262 0.006828 0.008018
Nuclear Periphery 0.000253 0.000192 0.001615 0.001257 0.000191 0.000158 0.000597 0.000410 0.000224 0.000172 0.000670 0.000894
Nuclear Periphery Foci 0.001757 0.002000 0.010554 0.003822 0.003034 0.000527 0.002999 0.002398 0.002356 0.001124 0.003538 0.003211
Nucleolus 0.000329 0.000882 0.000399 0.000559 0.000311 0.000343 0.000146 0.000117 0.000320 0.000562 0.000164 0.000369
Nucleus 0.085602 0.100254 0.311046* 0.109544 0.099562 0.114061 0.213331* 0.157791* 0.092156 0.108462 0.220299* 0.130236*
Peroxisomes 0.001184 0.001102 0.000334 0.001572 0.001106 0.001651 0.000095 0.000773 0.001147 0.001429 0.000112 0.001229
Vacuole 0.068043 0.044914 0.126886 0.138140 0.039188 0.042247 0.053631 0.071799 0.054495 0.043329 0.058855 0.109687
Vacuole Periphery 0.000814 0.000752 0.000261 0.002688 0.000445 0.000643 0.000095 0.001664 0.000641 0.000687 0.000107 0.002249

Localization Changes (T-score)

R1 R2 R1 & R2
UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14 UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14 UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14
Actin 1.53 -5.44 -2.11 -3.20 3.33 0.31 -0.06 -2.99 -3.51 -3.73 1.30 -0.88 -3.07 -4.14 -2.80
Bud Neck 0.27 -2.94 -2.38 -2.18 1.05 -3.70 -0.03 -3.25 0.66 -4.41 -3.41 1.40 -4.04 -0.12 -6.19
Bud Site -2.07 -2.87 -4.75 -2.97 -2.32 -3.77 0.27 -4.79 -2.13 -5.14 -4.36 0.58 -6.74 -3.24 -6.88
Cell Periphery 7.47 -6.23 5.30 -2.62 7.33 5.11 4.23 3.12 -3.71 -1.96 8.47 3.49 5.63 -4.37 2.38
Cytoplasm -10.28 10.75 14.28 25.70 -5.16 1.52 15.35 19.78 20.35 6.78 -6.65 13.78 23.77 32.68 8.39
Cytoplasmic Foci 16.19 24.41 29.02 16.94 2.40 3.71 20.71 20.20 23.92 -1.10 14.94 34.72 34.59 29.33 -0.98
Eisosomes 11.63 -2.26 8.97 -7.09 7.00 2.91 5.67 4.37 1.99 -2.06 11.07 9.82 9.78 -3.21 -1.06
Endoplasmic Reticulum -0.39 -7.43 0.59 0.67 7.50 6.48 -1.97 2.27 -9.95 6.60 3.38 -6.03 1.81 -2.13 7.79
Endosome 5.41 1.40 5.47 1.14 3.92 2.05 8.24 4.11 2.57 -1.23 5.49 10.71 6.76 2.55 -1.83
Golgi 1.33 4.97 0.41 -0.34 -1.71 -0.51 3.07 0.77 1.23 -1.45 0.08 5.82 0.79 0.72 -2.61
Lipid Particles 6.43 -1.46 5.41 -0.85 5.76 3.93 4.22 2.96 -2.61 -4.38 7.23 7.35 5.78 -2.30 -2.20
Mitochondria 2.11 2.23 2.12 0.04 -0.97 -1.04 1.22 0.21 1.72 -1.84 1.39 2.66 2.06 1.83 -3.03
Mitotic Spindle -0.20 -1.49 -7.74 -7.82 -3.86 -4.74 -4.62 -7.20 -3.06 -5.99 -4.00 -2.77 -10.13 -7.58 -9.16
None -5.56 1.10 -7.06 2.30 -8.14 0.26 -1.43 -3.43 -3.60 -3.59 -3.26 -5.11 -6.17 -0.86 -1.51
Nuclear Periphery 1.04 -8.83 -9.05 -10.21 4.12 2.36 -19.83 -18.41 -19.43 -0.38 1.76 -17.93 -13.78 -15.42 -1.41
Nuclear Periphery Foci -1.20 -6.71 -1.32 -0.55 6.05 6.81 -0.24 1.61 -7.62 2.75 5.95 -5.03 0.19 -4.51 4.52
Nucleolus -1.78 -0.90 -0.74 1.60 0.42 -0.66 5.20 3.18 2.84 -2.91 -1.87 6.48 0.39 1.94 -3.48
Nucleus -3.96 -10.70 -14.26 -10.41 7.68 -3.22 -26.07 -16.77 -14.88 7.41 -5.52 -31.98 -21.53 -17.01 15.09
Peroxisomes 0.24 4.88 2.82 1.66 -1.32 -1.19 2.87 2.06 4.14 -2.99 -1.03 5.46 3.21 4.12 -3.88
Vacuole 5.06 -6.93 -25.39 -28.62 -2.44 -1.01 -12.92 -19.82 -20.02 -10.79 3.93 -11.86 -32.21 -35.23 -18.83
Vacuole Periphery 0.34 3.04 0.19 -0.04 -1.56 -1.27 4.37 -0.04 0.55 -1.23 -0.29 5.61 0.07 0.24 -2.52
Standard name
Human Ortholog
Description FMN-dependent NAD(P)H:quinone reductase; role in apoptosis-like cell death; may be involved in quinone detoxification; expression elevated at low temperature; sequesters the Cin5p transcription factor in the cytoplasm in complex with the proteasome under reducing conditions
Localization
Cell Percentages cytoplasm (91%)
Cell Cycle Regulation No
Subcompartmental Group cytoplasm-9

Lot6

Lot6


Nearest neighbours ( genes)

correlation threshold

0.6

1.0

0.80


Biological Process (Gene Ontology)
Molecular Function (Gene Ontology)
Cellular Component (Gene Ontology)

Endocytosis
Temp Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Vph1-GFP)
37℃
RT
Cell Cycle Omics

CYCLoPs (Lot6-GFP)
Gene / Allele Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Sac6-tdTomato)
Gene Images
Gene Images
Images are not yet available
Images are not yet available