Standard name
Human Ortholog
Description Subunit of COMPASS (Set1C) complex; COMPASS methylates Lys4 of histone H3 and functions in silencing at telomeres; has a C-terminal Sdc1 Dpy-30 Interaction (SDI) domain that mediates binding to Sdc1p; similar to trithorax-group protein ASH2L
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Micrographs

ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches – 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud – 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Neck – 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site – 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 – – – – – –
Cell Periphery – 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cytoplasm – 0.07 0 0.06 0.08 0.13 0.08 0.11 – 0.1 0.06 0.06 0.07 0.14 0.2 0.06 0 0.05 0 0 0 0 0 0
Endoplasmic Reticulum – 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Endosome – 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Golgi – 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Mitochondria – 0 0.63 0.31 0.52 0.72 0.79 0.63 – 0.66 0.78 0.79 0 0 0 0.08 0.07 0.06 0 0 0 0 0 0
Nucleus – 0.79 0.69 0.77 0.62 0.52 0.5 0.5 – 0.51 0.54 0.53 0.9 0.83 0.77 0.88 0.9 0.86 0.71 0.71 0.62 0.67 0.63 0.65
Nuclear Periphery – 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus – 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0.05 0.07 0.07 0.1 0.07 0.07 0.07
Peroxisomes – 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole – 0 0 0 0 0 0 0 – 0 0 0 0.07 0 0 0 0 0 0 0 0.06 0 0.05 0
Vac/Vac Membrane – 0 0 0 0.05 0 0 0 – 0.05 0 0 0 0 0 0 0 0 0.1 0.1 0.08 0.1 0.12 0.13
ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches – 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 1 0 0 0 0 0 0
Bud – 0 0 0 0 5 10 13 – 9 8 18 0 0 0 1 0 1 0 2 1 1 3 5
Bud Neck – 0 0 0 0 0 0 0 – 0 1 0 1 2 1 0 0 0 0 0 1 0 2 1
Bud Site – 0 1 0 2 15 22 9 – 15 22 21 0 0 0 0 0 1 – – – – – –
Cell Periphery – 0 0 0 0 0 0 1 – 0 0 1 2 0 0 0 2 0 0 0 0 0 0 0
Cytoplasm – 2 3 19 35 80 69 93 – 67 35 45 13 36 66 21 15 19 1 1 2 0 5 8
Endoplasmic Reticulum – 1 1 0 0 0 0 0 – 0 0 0 1 2 1 7 5 7 0 0 0 0 1 2
Endosome – 1 0 3 4 1 1 15 – 3 1 0 0 1 2 3 2 4 0 1 4 1 3 6
Golgi – 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Mitochondria – 1 61 99 220 443 648 539 – 452 431 569 6 6 3 29 30 21 0 0 2 0 4 3
Nucleus – 23 67 248 261 320 411 421 – 350 298 379 179 209 257 322 374 312 28 51 66 36 126 200
Nuclear Periphery – 0 0 1 0 2 2 3 – 1 3 3 0 0 0 1 2 3 0 0 0 0 0 0
Nucleolus – 1 2 10 7 8 9 24 – 14 7 18 0 0 0 15 15 17 2 5 10 3 14 20
Peroxisomes – 0 0 0 0 0 0 0 – 0 0 0 0 0 0 1 1 0 0 0 0 0 0 0
SpindlePole – 1 2 5 5 6 1 13 – 9 4 7 15 9 9 8 4 8 1 2 6 1 10 10
Vac/Vac Membrane – 1 0 5 22 18 24 21 – 34 16 19 2 1 5 12 12 14 4 6 8 5 24 41
Unique Cell Count 29 97 323 421 612 817 849 688 550 718 200 253 334 368 414 361 40 72 106 54 202 311
Labelled Cell Count 31 137 390 556 898 1197 1152 954 826 1080 219 266 344 420 462 408 40 72 106 54 202 311


Nucleus

(Huh et al. Nature 2003 425:686)
Screen WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 AF100 AF140 AF180
Mean Cell GFP Intensity (1e-4) – 5.1 3.7 4.2 3.7 3.5 3.0 3.4 – 3.2 3.3 3.1 5.3 5.2 5.2 6.8 6.6 6.9 5.1 5.7 6.0
Std Deviation (1e-4) – 1.2 0.9 1.3 1.2 1.3 1.1 1.4 – 1.1 1.4 0.7 0.9 0.9 1.2 1.2 1.2 1.4 1.3 1.5 1.6
Intensity Change (Log2) – – – 0.18 0.01 -0.1 -0.3 -0.13 – -0.23 -0.17 -0.26 0.51 0.47 0.5 0.86 0.82 0.9 0.47 0.63 0.69


Localization RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3
Cortical Patches 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0
Bud 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0
Cell Periphery 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0
Cytoplasm 1.1 1.8 2.8 1.9 2.4 – 2.2 1.3 1.2 1.2 3.0 3.9 1.0 0 0
Endoplasmic Reticulum 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0
Endosome 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0
Golgi 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0
Mitochondria 0 0 0 0 0 – 0 0 0 -11.6 -12.9 -15.1 -12.2 -12.9 -13.0
Nucleus 1.5 -1.3 -3.1 -3.5 -3.6 – -3.4 -2.7 -3.0 4.4 2.8 1.6 4.4 5.5 4.0
Nuclear Periphery 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 – 0 0 0 1.9 0 0 0 0 0
Vacuole 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0

Images

Circle plot not available

Protein Concentration and Protein Localization Data

R1 R2 R3
G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase
Concentration 0.4284 0.6932 0.4743 0.2275 0.5347 0.6032 0.3682 0.791 0.5608 0.7468 0.8085 0.5588 -1.0673 -0.894 -1.2939 -0.6502 -1.4259 -0.8459
Actin 0.0052 0.0001 0.0072 0 0.0118 0.0005 0.0091 0.0001 0.0008 0.0273 0.0049 0.0002 0.0124 0 0.0001 0.0024 0 0.0082
Bud 0.0001 0.0001 0 0 0.0001 0 0.0002 0 0.0002 0.0005 0.0001 0.0001 0.0003 0 0 0.0067 0.0001 0.0003
Bud Neck 0.0005 0.0005 0.0002 0.0001 0.0019 0.0012 0.0011 0.0004 0.0004 0.0016 0.0023 0.002 0.0003 0.0005 0.0002 0.0021 0.001 0.0031
Bud Periphery 0.0003 0.0001 0 0 0.0002 0 0.0004 0 0.0003 0.0017 0.0004 0.0004 0.0003 0 0 0.0271 0.0001 0.0003
Bud Site 0.0002 0.0004 0.0001 0 0.0004 0 0.0016 0.0003 0.0009 0.0009 0.0004 0.0002 0.0029 0.0004 0.0003 0.0053 0.0002 0.0007
Cell Periphery 0 0 0 0 0.0001 0 0.0004 0 0.0001 0.0002 0.0001 0.0001 0.0001 0 0 0.0011 0 0
Cytoplasm 0.0033 0.0082 0.0009 0.0002 0.008 0.0017 0.0081 0.003 0.0051 0.0008 0.0071 0.004 0.0175 0.0018 0.0023 0.0113 0.0004 0.0018
Cytoplasmic Foci 0.0003 0.0002 0.0001 0 0.0038 0.0033 0.0066 0.0002 0.0007 0.0055 0.0029 0.0007 0.0366 0.0001 0 0.0016 0 0.0236
Eisosomes 0.0001 0 0.0002 0 0.0003 0 0.0002 0 0 0.0003 0.0002 0 0.0001 0 0 0.0001 0 0
Endoplasmic Reticulum 0.0002 0.0003 0 0 0.0023 0.0002 0.0037 0.0001 0.0014 0.0019 0.0024 0.0002 0.0017 0.0001 0.0003 0.0108 0 0.0003
Endosome 0.0006 0.0004 0.0001 0 0.0251 0.0007 0.013 0.0001 0.0076 0.007 0.0136 0.0017 0.0124 0.0001 0.0002 0.0166 0.0001 0.0141
Golgi 0.0003 0 0.0003 0 0.0051 0.0001 0.0018 0 0.0008 0.0044 0.0018 0.0009 0.0045 0 0 0.0012 0 0.0139
Lipid Particles 0.0003 0 0.0002 0 0.0121 0.0001 0.0077 0.0001 0.0007 0.017 0.0169 0.0062 0.0074 0 0 0.0008 0 0.0032
Mitochondria 0.0007 0.0002 0.0001 0 0.0016 0.0002 0.0019 0.0002 0.0011 0.0051 0.0041 0.0021 0.0018 0.0001 0.0002 0.0145 0.0004 0.0098
None 0.0095 0.0036 0.0008 0.0001 0.072 0.0033 0.0268 0.0389 0.029 0.0033 0.0206 0.0166 0.0458 0.0128 0.0011 0.0027 0.0001 0.0007
Nuclear Periphery 0.0036 0.011 0.0018 0.0008 0.0214 0.0065 0.0172 0.007 0.0074 0.024 0.027 0.0062 0.002 0.0039 0.0127 0.0207 0.0025 0.0098
Nucleolus 0.0185 0.0215 0.0127 0.0088 0.0171 0.0305 0.0171 0.0187 0.0121 0.0143 0.0504 0.0354 0.0171 0.025 0.0227 0.0147 0.0448 0.0499
Nucleus 0.9296 0.9317 0.9468 0.982 0.7404 0.9259 0.8362 0.9098 0.9129 0.8623 0.8055 0.9029 0.8139 0.9332 0.9496 0.7551 0.8953 0.8195
Peroxisomes 0.0002 0 0.0002 0 0.0018 0.0001 0.0036 0 0.0005 0.0103 0.0078 0.0002 0.0132 0 0 0.0004 0 0.0218
Punctate Nuclear 0.0261 0.0203 0.028 0.008 0.071 0.0254 0.0314 0.0208 0.0149 0.0091 0.0291 0.0192 0.0089 0.0217 0.0096 0.0699 0.0547 0.0184
Vacuole 0.0004 0.0012 0 0 0.0018 0.0001 0.0102 0.0002 0.0023 0.0011 0.0016 0.0006 0.0005 0.0002 0.0003 0.027 0.0001 0.0003
Vacuole Periphery 0.0001 0.0002 0 0 0.0015 0.0001 0.0018 0 0.0005 0.0013 0.0008 0.0002 0.0003 0 0.0002 0.0081 0.0001 0.0002

Sequencing Data

R1 R2
G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Post-START S/G2 Metaphase Anaphase Telophase
Gene Expression 9.8129 6.4979 12.5364 8.914 12.2516 7.2648 9.198 9.0903 8.1013 9.9317
Translational Efficiency 1.3849 1.755 0.6389 0.9612 0.8632 1.0963 0.9882 0.9826 0.9951 0.9498

Hit Data

Dataset Hit
Protein Concentration ✔
Protein Localization ✘
Gene Expression ✘
Translational Efficiency ✘

Micrographs

Cell Count

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
920 1441 180 713 1109 1043 797 520 2029 2484 977 1233

Protein Abundance

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
Mean 693.62 739.28 979.03 913.22 625.26 721.26 838.55 954.77 656.26 731.71 864.43 930.74
Standard Deviation 78.17 108.08 90.65 115.28 95.86 95.48 115.75 124.50 94.61 103.36 124.14 121.01
Intensity Change Log 2 — 0.091975 0.497208 0.396817 — 0.206063 0.423441 0.610697 — 0.147189 0.462706 0.502173

Localization Score (DeepLoc)

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
Actin 0.000169 0.000476 0.002224 0.001421 0.000201 0.000386 0.001103 0.002112 0.000186 0.000438 0.001309 0.001712
Bud Neck 0.001206 0.004117 0.011085 0.014689 0.001902 0.005103 0.015510 0.016306 0.001586 0.004531 0.014695 0.015371
Bud Site 0.001358 0.001457 0.002430 0.015606 0.000576 0.002348 0.001775 0.014282 0.000930 0.001831 0.001896 0.015047
Cell Periphery 0.000108 0.000130 0.000114 0.000187 0.000148 0.000148 0.000090 0.000137 0.000130 0.000137 0.000095 0.000166
Cytoplasm 0.134152* 0.040667 0.005735 0.037051 0.104082* 0.020947 0.004977 0.020004 0.117716* 0.032387 0.005116 0.029862
Cytoplasmic Foci 0.025355 0.005541 0.000183 0.005365 0.019792 0.010436 0.000091 0.002494 0.022314 0.007597 0.000108 0.004154
Eisosomes 0.000039 0.000033 0.000069 0.000039 0.000045 0.000042 0.000028 0.000037 0.000042 0.000036 0.000035 0.000038
Endoplasmic Reticulum 0.000889 0.003251 0.012035 0.007889 0.001595 0.002629 0.004682 0.008024 0.001275 0.002990 0.006037 0.007946
Endosome 0.001271 0.001019 0.000669 0.007177 0.001067 0.001274 0.000311 0.004715 0.001159 0.001126 0.000377 0.006138
Golgi 0.000578 0.000393 0.000194 0.003312 0.000333 0.001976 0.000131 0.006904 0.000444 0.001058 0.000143 0.004827
Lipid Particles 0.004038 0.002313 0.000652 0.001289 0.002878 0.004204 0.000184 0.000454 0.003404 0.003107 0.000271 0.000937
Mitochondria 0.002196 0.005446 0.001404 0.004349 0.001992 0.009984 0.001162 0.004048 0.002084 0.007351 0.001206 0.004222
Mitotic Spindle 0.002636 0.003426 0.001673 0.062057 0.000564 0.006212 0.002141 0.014893 0.001503 0.004596 0.002055 0.042166
None 0.011852 0.013894 0.002745 0.012803 0.036599 0.011650 0.005871 0.007759 0.025378 0.012951 0.005295 0.010676
Nuclear Periphery 0.001171 0.001408 0.000745 0.002615 0.000971 0.001426 0.000511 0.001111 0.001062 0.001415 0.000554 0.001980
Nuclear Periphery Foci 0.000699 0.000938 0.000558 0.003360 0.001052 0.000869 0.000445 0.000925 0.000892 0.000909 0.000466 0.002333
Nucleolus 0.006350 0.020006 0.020888 0.007145 0.015968 0.031639 0.014676 0.008036 0.011607 0.024890 0.015820 0.007521
Nucleus 0.801197* 0.891120* 0.930385* 0.770374* 0.806395* 0.881943* 0.944624* 0.874586* 0.804038* 0.887266* 0.942001* 0.814324*
Peroxisomes 0.001493 0.001977 0.000643 0.000375 0.001255 0.003560 0.000289 0.002654 0.001363 0.002642 0.000354 0.001336
Vacuole 0.003007 0.001931 0.005334 0.030115 0.002343 0.002553 0.001245 0.009677 0.002644 0.002192 0.001998 0.021496
Vacuole Periphery 0.000235 0.000458 0.000234 0.012781 0.000243 0.000673 0.000154 0.000841 0.000239 0.000548 0.000169 0.007746

Localization Changes (T-score)

R1 R2 R1 & R2
UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14 UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14 UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14
Actin -4.62 -11.94 -15.43 -13.69 3.36 -4.09 -14.27 -6.76 -6.01 -2.27 -6.44 -17.50 -13.73 -11.67 -2.32
Bud Neck -7.34 -6.18 -16.31 -12.33 -0.56 -3.96 -13.40 -9.94 -8.10 0.26 -6.75 -15.30 -17.10 -13.77 1.16
Bud Site -0.55 -2.97 -4.86 -4.89 -3.43 -2.10 -8.34 -3.82 -3.03 -2.98 -2.09 -5.50 -6.13 -5.26 -4.71
Cell Periphery -1.11 -1.04 -2.24 -0.36 -0.90 0.04 3.87 1.93 1.09 -1.81 -0.42 3.06 0.17 0.53 -2.97
Cytoplasm 14.57 20.87 16.90 3.60 -6.34 14.10 18.25 14.34 1.20 -5.27 19.36 28.16 21.55 3.69 -9.50
Cytoplasmic Foci 8.49 11.23 10.41 4.71 -1.86 3.98 10.62 9.48 5.49 -2.71 9.17 15.46 14.04 7.22 -3.17
Eisosomes 2.48 -4.51 -7.06 -9.42 0.49 1.26 5.34 -1.63 -2.85 -6.57 2.92 2.30 -6.37 -9.21 -8.33
Endoplasmic Reticulum -11.28 -14.33 -19.61 -14.46 0.32 -4.25 -12.72 -10.09 -8.83 -6.17 -10.74 -18.58 -20.12 -16.14 -8.99
Endosome 1.21 2.44 -2.05 -2.81 -3.50 -0.46 5.51 -1.68 -1.54 -2.78 0.70 6.56 -2.71 -2.99 -4.89
Golgi 0.43 0.96 -0.53 -1.34 -2.03 -2.42 1.89 -1.42 -0.42 -1.55 -1.89 1.75 -1.57 -0.70 -2.02
Lipid Particles 3.36 6.72 6.01 3.81 -1.09 -1.37 10.23 7.82 4.12 -3.96 0.99 11.74 9.00 5.46 -4.36
Mitochondria -2.24 0.59 -0.54 2.42 -3.25 -4.10 0.93 -0.85 3.72 -2.07 -4.63 1.13 -1.04 4.41 -4.01
Mitotic Spindle -0.66 -0.86 -6.76 -6.60 -5.98 -3.56 -2.62 -4.48 -3.03 -3.52 -3.04 -2.17 -8.20 -7.18 -7.15
None -1.12 8.08 3.23 4.67 -4.82 7.61 10.43 9.82 3.99 -0.95 6.50 11.79 10.08 6.14 -2.84
Nuclear Periphery -0.85 1.54 -7.71 -5.85 -9.17 -1.29 1.16 -3.00 -1.35 -7.67 -1.58 2.29 -7.48 -5.52 -12.69
Nuclear Periphery Foci -0.90 0.17 -2.18 -1.79 -2.22 1.07 3.38 -0.44 -0.90 -1.69 0.22 3.28 -1.68 -1.86 -3.31
Nucleolus -9.25 -3.11 2.39 11.17 3.68 -6.19 -0.41 6.82 10.24 6.29 -9.44 -3.50 7.51 14.86 8.15
Nucleus -9.80 -11.23 -2.59 6.02 7.96 -7.12 -15.28 -4.78 1.12 6.74 -12.08 -20.49 -4.86 5.32 12.14
Peroxisomes -1.05 2.28 3.78 4.54 3.33 -3.09 3.62 0.33 2.76 -1.58 -2.92 4.80 2.47 4.85 -1.19
Vacuole 1.83 -3.42 -9.42 -9.82 -8.45 -0.17 2.76 -5.90 -5.82 -6.19 1.20 1.11 -11.12 -11.32 -11.32
Vacuole Periphery -2.07 -0.30 -2.01 -1.82 -1.99 -2.95 2.52 -1.41 0.96 -1.96 -3.66 1.70 -2.14 -1.67 -2.24
Standard name
Human Ortholog
Description Subunit of COMPASS (Set1C) complex; COMPASS methylates Lys4 of histone H3 and functions in silencing at telomeres; has a C-terminal Sdc1 Dpy-30 Interaction (SDI) domain that mediates binding to Sdc1p; similar to trithorax-group protein ASH2L
Localization
Cell Percentages nucleus (93%)
Cell Cycle Regulation No
Subcompartmental Group nucleus-3

Bre2

Bre2


Nearest neighbours ( genes)

correlation threshold

0.6

1.0

0.80


Biological Process (Gene Ontology)
Molecular Function (Gene Ontology)
Cellular Component (Gene Ontology)

Endocytosis
Temp Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Vph1-GFP)
37℃
RT
Cell Cycle Omics

CYCLoPs (Bre2-GFP)
Gene / Allele Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Sac6-tdTomato)
Gene Images
Gene Images
Images are not yet available
Images are not yet available