Standard name
Human Ortholog
Description Subunit of the RES complex; RES complex is required for nuclear retention of unspliced pre-mRNAs; acts in the same pathway as Pml39p and Mlp1p
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Micrographs

ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 – 0 0 0.06 0 0 0 0 0 0 0 – – – – – –
Cell Periphery 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cytoplasm 0 0.06 0.13 0.11 0.09 0.15 0.13 – 0.05 0.08 0 0.06 0.2 0.25 0.28 0.15 0.15 0.15 0 0 0 0.06 0 0
Endoplasmic Reticulum 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0.05 0 0
Endosome 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Golgi 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Mitochondria 0.07 0.14 0 0 0 0.42 0.13 – 0.5 0.44 0.55 0.43 0 0 0 0 0 0 0 0 0 0 0 0
Nucleus 0.91 0.86 0.78 0.83 0.81 0.63 0.76 – 0.73 0.69 0.78 0.81 0.77 0.7 0.68 0.74 0.74 0.77 0.82 0.84 0.71 0.59 0.53 0.52
Nuclear Periphery 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Vac/Vac Membrane 0 0 0 0 0.07 0 0 – 0 0 0 0 0 0.05 0.08 0.11 0.14 0.07 0.1 0.08 0.12 0.18 0.25 0.25
ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 1 2 0 0 0 0 – 0 0 0 0 0 0 0 1 0 0 0 3 2 1 3 1
Bud 0 1 0 0 0 3 0 – 4 4 4 1 0 0 0 0 0 1 4 2 0 2 5 5
Bud Neck 0 0 4 0 0 1 0 – 0 0 0 0 0 0 0 0 0 0 0 3 0 0 2 4
Bud Site 0 1 0 0 1 1 0 – 3 10 12 8 0 0 0 0 0 0 – – – – – –
Cell Periphery 0 1 1 1 1 0 1 – 1 1 0 0 0 4 4 8 4 11 0 0 1 0 0 1
Cytoplasm 9 28 20 13 16 34 36 – 11 20 7 12 67 81 129 76 56 62 5 9 8 10 9 5
Endoplasmic Reticulum 1 3 1 0 0 0 0 – 1 0 0 3 1 5 1 12 11 15 1 5 4 8 12 8
Endosome 6 0 1 4 1 1 5 – 1 1 0 1 2 3 3 4 4 1 9 1 2 2 3 9
Golgi 1 0 0 0 0 0 0 – 0 0 0 0 0 0 1 1 0 1 2 0 1 0 3 1
Mitochondria 32 68 1 5 5 92 36 – 110 111 110 93 1 1 0 7 7 13 4 9 5 5 10 8
Nucleus 421 420 124 100 152 140 211 – 159 173 156 176 259 230 314 377 282 308 419 419 135 102 174 166
Nuclear Periphery 10 0 0 0 1 0 1 – 4 2 0 1 1 0 0 2 0 0 0 0 0 0 1 4
Nucleolus 16 0 0 0 0 1 1 – 1 0 1 0 0 1 2 0 0 1 1 0 0 3 10 10
Peroxisomes 1 0 0 0 0 0 0 – 0 0 0 0 0 1 1 0 0 0 2 0 0 0 0 0
SpindlePole 0 2 4 0 3 0 5 – 1 2 1 4 0 1 2 5 0 0 3 3 1 2 7 8
Vac/Vac Membrane 15 20 7 2 14 8 11 – 4 5 4 4 10 17 37 56 52 29 53 37 23 30 81 80
Unique Cell Count 465 488 159 120 187 221 277 219 251 201 218 335 330 464 507 379 401 512 501 191 175 332 323
Labelled Cell Count 512 545 165 125 194 281 307 300 329 295 303 341 344 494 549 416 442 512 501 191 175 332 323


Cytoplasm, Nucleus

(Huh et al. Nature 2003 425:686)
Screen WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 AF100 AF140 AF180
Mean Cell GFP Intensity (1e-4) 5.0 4.6 5.7 5.2 5.9 4.0 4.8 – 4.1 4.2 4.1 4.3 6.0 6.2 6.1 7.2 7.6 6.9 5.5 5.6 5.6
Std Deviation (1e-4) 4.2 0.7 1.0 1.4 1.4 1.2 1.4 – 1.0 0.7 0.7 1.0 1.4 1.7 1.5 1.6 1.6 1.5 2.0 1.9 1.5
Intensity Change (Log2) – – – -0.14 0.05 -0.51 -0.25 – -0.48 -0.45 -0.48 -0.4 0.06 0.11 0.1 0.33 0.41 0.27 -0.06 -0.04 -0.03


Localization RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3
Actin – – – – – – – – – – – – – – –
Bud – – – – – – – – – – – – 0 0 0
Bud Neck – – – – – – – – – – – – 0 0 0
Bud Site – – – – – – – – – – – – 0 0 0
Cell Periphery – – – – – – – – – – – – 0 0 0
Cyto – – – – – – – – – – – – – – –
Endoplasmic Reticulum – – – – – – – – – – – – 0 0 0
Endosome – – – – – – – – – – – – 0 0 0
Golgi – – – – – – – – – – – – 0 0 0
Mitochondria – – – – – – – – – – – – 0 0 0
Nuclear Periphery – – – – – – – – – – – – 0 0 0
Nuc – – – – – – – – – – – – – – –
Nucleolus – – – – – – – – – – – – 0 0 0
Peroxisomes – – – – – – – – – – – – 0 0 0
SpindlePole – – – – – – – – – – – – 0 0 0
Vac – – – – – – – – – – – – – – –
Cortical Patches – – – – – – – – – – – – 0 0 0
Cytoplasm – – – – – – – – – – – – 0 0 0
Nucleus – – – – – – – – – – – – 0 0 0
Vacuole – – – – – – – – – – – – 2.5 3.2 1.2

Images

Circle plot not available

Protein Concentration and Protein Localization Data

R1 R2 R3
G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase
Concentration 1.3291 0.6045 0.2998 0.535 0.1671 0.3708 2.2859 1.525 1.8749 2.0635 1.4649 1.8269 4.2203 5.0778 4.3715 3.8484 4.7572 4.5586
Actin 0.0281 0.0022 0.007 0.006 0.0446 0.0096 0.0626 0.0019 0.0329 0.0694 0.025 0.0053 0.0321 0.0033 0.0072 0.0032 0.001 0.0013
Bud 0.0008 0.001 0.0007 0.014 0.0026 0.0007 0.0009 0.0029 0.0022 0.001 0.0011 0.0003 0.0004 0.0005 0.0004 0.0003 0.0001 0.0001
Bud Neck 0.001 0.0003 0.0005 0.0074 0.0023 0.0078 0.0044 0.0008 0.0041 0.0061 0.0063 0.004 0.0033 0.0006 0.0005 0.0054 0.0004 0.0057
Bud Periphery 0.002 0.0009 0.0007 0.0285 0.0049 0.0007 0.0013 0.0043 0.003 0.0018 0.0021 0.0005 0.0006 0.0011 0.0005 0.001 0.0001 0.0002
Bud Site 0.0026 0.0095 0.0017 0.0453 0.0274 0.0021 0.0062 0.0282 0.0161 0.0074 0.0025 0.0047 0.0051 0.0076 0.0022 0.0005 0.0004 0.0001
Cell Periphery 0.0016 0.0011 0.0011 0.0023 0.0016 0.0006 0.0015 0.0028 0.0023 0.0008 0.0007 0.0006 0.0038 0.0044 0.0024 0.0024 0.0021 0.0007
Cytoplasm 0.3823 0.5343 0.4354 0.3977 0.5325 0.6542 0.3184 0.4635 0.5068 0.4245 0.4878 0.5463 0.5675 0.576 0.6178 0.7709 0.5765 0.691
Cytoplasmic Foci 0.0414 0.0109 0.0195 0.0126 0.0163 0.0228 0.0222 0.0148 0.0201 0.0169 0.0523 0.0189 0.0181 0.0162 0.0121 0.0082 0.0096 0.014
Eisosomes 0.0006 0.0002 0.0002 0.0004 0.0007 0.0001 0.0009 0.0004 0.0007 0.0019 0.0005 0.0002 0.0005 0.0004 0.0003 0.0002 0.0002 0.0001
Endoplasmic Reticulum 0.0659 0.0289 0.0456 0.0331 0.0944 0.0307 0.0285 0.0275 0.0243 0.0537 0.0605 0.0259 0.0727 0.0411 0.0613 0.0671 0.0219 0.0629
Endosome 0.0447 0.0083 0.0288 0.006 0.0183 0.016 0.0245 0.0142 0.0075 0.0161 0.0304 0.016 0.0195 0.0161 0.0111 0.0034 0.0018 0.0099
Golgi 0.0084 0.0012 0.003 0.0013 0.006 0.0016 0.0071 0.0016 0.0029 0.0042 0.0041 0.0026 0.0058 0.0036 0.0022 0.0012 0.0003 0.0006
Lipid Particles 0.0101 0.0012 0.0055 0.0038 0.014 0.0019 0.0078 0.0025 0.0032 0.01 0.0033 0.0043 0.0077 0.0023 0.0035 0.0008 0.0016 0.0009
Mitochondria 0.005 0.0005 0.0014 0.0023 0.0285 0.0007 0.0064 0.0014 0.0028 0.0377 0.0043 0.0021 0.0047 0.0064 0.0012 0.0003 0.0001 0.0004
None 0.3025 0.3881 0.4193 0.4174 0.1596 0.2433 0.4412 0.4155 0.3538 0.3295 0.2795 0.3531 0.2468 0.3095 0.2656 0.1309 0.3756 0.2068
Nuclear Periphery 0.0399 0.0025 0.0118 0.0027 0.0046 0.0017 0.0217 0.0027 0.0018 0.0033 0.0135 0.0029 0.0028 0.0025 0.0033 0.0009 0.0009 0.0014
Nucleolus 0.0009 0.0004 0.0003 0.0018 0.0097 0.0001 0.0013 0.0009 0.0005 0.0007 0.0007 0.0002 0.0003 0.0003 0.0002 0.0001 0.0009 0.0001
Nucleus 0.023 0.0023 0.0058 0.0054 0.0061 0.0017 0.0209 0.004 0.0023 0.0026 0.0064 0.0028 0.001 0.0024 0.0015 0.0008 0.0013 0.0013
Peroxisomes 0.0111 0.0001 0.0034 0.0011 0.0143 0.0004 0.0109 0.0003 0.007 0.0057 0.0067 0.0049 0.0022 0.0004 0.0004 0.0001 0.0001 0.0001
Punctate Nuclear 0.021 0.0016 0.0027 0.0012 0.0057 0.0011 0.0064 0.004 0.0021 0.0028 0.0047 0.0027 0.0019 0.0011 0.0023 0.0003 0.0034 0.0005
Vacuole 0.0046 0.004 0.0048 0.0091 0.0048 0.0019 0.0035 0.0052 0.0033 0.0031 0.0063 0.0015 0.0027 0.0038 0.0037 0.002 0.0018 0.0018
Vacuole Periphery 0.0024 0.0004 0.0008 0.0009 0.0011 0.0002 0.0018 0.0006 0.0003 0.0009 0.0013 0.0004 0.0005 0.0006 0.0004 0.0001 0.0001 0.0002

Sequencing Data

R1 R2
G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Post-START S/G2 Metaphase Anaphase Telophase
Gene Expression 12.5591 16.0386 14.2816 13.7816 5.8155 8.9658 18.6491 12.0397 13.7119 16.5018
Translational Efficiency 1.0245 0.8905 0.8473 1.0964 2.3938 1.6498 0.7852 1.0757 1.1598 0.7509

Hit Data

Dataset Hit
Protein Concentration ✘
Protein Localization ✘
Gene Expression ✘
Translational Efficiency ✘

Micrographs

Cell Count

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
1251 1288 2428 2161 1595 2135 2423 2264 2846 3423 4851 4425

Protein Abundance

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
Mean 654.40 843.21 847.60 800.56 715.02 819.67 815.69 758.92 688.37 828.53 831.66 779.26
Standard Deviation 74.45 166.55 104.03 94.30 81.02 99.07 96.58 87.94 83.79 129.19 101.64 93.45
Intensity Change Log 2 — 0.365719 0.373211 0.290837 — 0.197060 0.190037 0.085964 — 0.280119 0.280475 0.187500

Localization Score (DeepLoc)

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
Actin 0.001359 0.002847 0.000318 0.001382 0.000782 0.002067 0.000972 0.000198 0.001036 0.002360 0.000645 0.000777
Bud Neck 0.030141 0.027548 0.011673 0.013462 0.019968 0.037856 0.010445 0.021496 0.024440 0.033978 0.011060 0.017573
Bud Site 0.024257 0.051516 0.022564 0.020738 0.012101 0.036000 0.014444 0.011449 0.017444 0.041839 0.018508 0.015986
Cell Periphery 0.002046 0.001291 0.000458 0.000872 0.000632 0.000728 0.000433 0.000312 0.001254 0.000940 0.000445 0.000585
Cytoplasm 0.315979* 0.345303* 0.504761* 0.481521* 0.373982* 0.383574* 0.551063* 0.472936* 0.348486* 0.369174* 0.527888* 0.477128*
Cytoplasmic Foci 0.249806* 0.286360* 0.026154 0.046741 0.294084* 0.245641* 0.050837 0.083910 0.274621* 0.260963* 0.038483 0.065758
Eisosomes 0.002604 0.000805 0.000073 0.000405 0.001040 0.001323 0.000179 0.000182 0.001727 0.001128 0.000126 0.000291
Endoplasmic Reticulum 0.076822 0.047594 0.004676 0.008454 0.045065 0.028951 0.007424 0.004627 0.059024 0.035966 0.006049 0.006496
Endosome 0.026137 0.034511 0.001886 0.003992 0.022865 0.025657 0.003452 0.014656 0.024303 0.028989 0.002668 0.009448
Golgi 0.040844 0.024526 0.000110 0.000623 0.022960 0.019767 0.000239 0.000511 0.030821 0.021558 0.000174 0.000566
Lipid Particles 0.060360 0.023196 0.001717 0.005319 0.041675 0.026784 0.003570 0.010091 0.049889 0.025434 0.002643 0.007761
Mitochondria 0.058122 0.011769 0.000600 0.000622 0.013280 0.015987 0.000495 0.000848 0.032991 0.014400 0.000547 0.000738
Mitotic Spindle 0.000351 0.001286 0.001358 0.002795 0.000696 0.001709 0.002572 0.001608 0.000544 0.001550 0.001965 0.002188
None 0.002963 0.001742 0.002734 0.001011 0.004167 0.002386 0.001841 0.002093 0.003638 0.002144 0.002288 0.001565
Nuclear Periphery 0.000922 0.000410 0.001126 0.001203 0.000695 0.000717 0.000782 0.001685 0.000794 0.000602 0.000954 0.001449
Nuclear Periphery Foci 0.001204 0.001932 0.002297 0.002621 0.001455 0.001219 0.002269 0.001738 0.001345 0.001487 0.002283 0.002169
Nucleolus 0.001014 0.000861 0.000395 0.000246 0.001073 0.000949 0.000264 0.001200 0.001047 0.000916 0.000330 0.000734
Nucleus 0.041497 0.037786 0.363301* 0.247369* 0.080036 0.074795 0.230915* 0.186573* 0.063096 0.060869 0.297176* 0.216263*
Peroxisomes 0.014789 0.009310 0.000269 0.001478 0.006993 0.009792 0.001000 0.002048 0.010420 0.009611 0.000634 0.001769
Vacuole 0.039396 0.082651 0.053228 0.158218 0.052988 0.079146 0.116650 0.181168 0.047013 0.080465 0.084906 0.169960
Vacuole Periphery 0.009386 0.006754 0.000302 0.000927 0.003464 0.004951 0.000154 0.000671 0.006067 0.005629 0.000228 0.000796

Localization Changes (T-score)

R1 R2 R1 & R2
UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14 UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14 UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14
Actin -3.61 7.27 0.02 3.27 -4.18 -4.95 -1.02 5.38 7.20 4.17 -5.88 3.32 1.78 6.45 -0.85
Bud Neck 0.90 7.31 6.87 7.37 -1.05 -8.00 6.48 -0.59 7.49 -7.26 -5.21 9.58 4.80 11.01 -6.24
Bud Site -7.55 0.87 1.96 9.19 0.98 -11.34 -1.10 0.62 11.29 1.57 -12.65 -0.25 1.63 13.78 1.73
Cell Periphery 2.66 8.77 6.71 2.11 -6.28 -1.34 4.89 7.95 6.75 4.32 2.56 9.65 8.18 4.08 -3.60
Cytoplasm -3.30 -21.31 -17.32 -13.09 3.84 -1.28 -19.86 -11.35 -10.55 8.09 -3.61 -28.40 -19.71 -16.25 8.40
Cytoplasmic Foci -4.81 44.48 39.98 40.84 -9.14 7.85 48.66 40.46 35.50 -12.14 2.94 65.88 56.72 54.63 -15.12
Eisosomes 7.76 11.25 9.84 6.41 -9.61 -2.12 14.65 14.75 9.49 -0.04 4.59 15.22 13.70 10.67 -8.89
Endoplasmic Reticulum 5.15 15.64 14.90 11.40 -4.79 4.71 12.63 13.85 13.13 3.74 7.41 20.07 20.08 16.91 -0.06
Endosome -4.21 19.89 18.08 19.36 -6.64 -1.84 18.33 6.45 8.48 -12.88 -3.90 27.05 16.66 19.99 -14.11
Golgi 7.79 23.08 22.72 20.31 -2.28 2.48 23.67 23.35 22.77 -4.34 7.90 31.97 31.45 30.36 -3.60
Lipid Particles 13.86 24.55 22.98 14.04 -9.23 7.96 24.22 19.37 14.66 -11.04 15.41 34.22 30.05 20.92 -14.21
Mitochondria 13.45 17.32 17.32 11.94 0.02 -1.81 11.38 11.03 15.04 -2.96 10.38 19.74 19.63 18.99 -2.22
Mitotic Spindle -1.94 -3.76 -3.44 -2.00 -1.64 -2.35 -2.80 -2.09 0.15 1.30 -3.13 -4.15 -3.97 -1.54 -0.35
None 2.07 0.70 3.89 2.10 5.69 1.78 2.70 2.23 0.52 -0.74 2.37 2.66 3.70 1.54 2.08
Nuclear Periphery 3.00 -2.23 -2.30 -6.51 -0.64 -0.15 -2.34 -9.81 -6.42 -9.71 1.60 -3.43 -7.61 -8.20 -6.62
Nuclear Periphery Foci -2.84 -6.22 -6.43 -2.32 -0.23 1.18 -3.14 -1.16 -2.88 2.38 -0.58 -6.02 -4.67 -4.17 1.40
Nucleolus 0.60 2.78 3.79 3.01 1.30 0.62 3.00 -0.19 -1.23 -5.11 0.85 3.94 1.70 1.30 -3.76
Nucleus 1.43 -57.56 -39.92 -41.09 16.82 1.57 -32.24 -23.58 -25.81 8.59 0.97 -61.45 -43.85 -45.42 18.27
Peroxisomes 5.91 20.83 18.08 11.74 -4.58 -4.70 16.93 13.40 15.37 -5.69 1.57 26.38 22.03 19.32 -7.19
Vacuole -11.85 -8.71 -29.37 -18.52 -23.03 -8.68 -21.50 -29.62 -23.16 -9.30 -14.48 -21.50 -41.46 -29.72 -22.35
Vacuole Periphery 3.35 15.72 12.47 8.88 -1.29 -3.43 14.49 12.07 11.59 -9.41 1.07 20.13 15.54 13.55 -2.59
Standard name
Human Ortholog
Description Subunit of the RES complex; RES complex is required for nuclear retention of unspliced pre-mRNAs; acts in the same pathway as Pml39p and Mlp1p
Localization
Cell Percentages ER (38%), cytoplasm (12%), mixed (47%)
Cell Cycle Regulation No
Subcompartmental Group N/A

Pml1

Pml1


Nearest neighbours ( genes)

correlation threshold

0.6

1.0

0.80


Biological Process (Gene Ontology)
Molecular Function (Gene Ontology)
Cellular Component (Gene Ontology)

Endocytosis
Temp Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Vph1-GFP)
37℃
RT
Cell Cycle Omics

CYCLoPs (Pml1-GFP)
Gene / Allele Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Sac6-tdTomato)
Gene Images
Gene Images
Images are not yet available
Images are not yet available