Standard name | |
---|---|
Human Ortholog | |
Description | Methylthioadenosine phosphorylase (MTAP); catalyzes the initial step in the methionine salvage pathway; affects polyamine biosynthesis through regulation of ornithine decarboxylase (Spe1p) activity; regulates ADH2 gene expression |
ensLOC | DeepLoc | |||||||||||||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Localization | WT1 | WT2 | WT3 | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 | WT1 | WT2 | WT3 | AF100 | AF140 | AF180 |
Cortical Patches | 0 | 0 | 0 | 1 | 1 | 0 | 0 | 2 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 2 | 2 |
Bud | 0 | 0 | 0 | 0 | 0 | 3 | 0 | 2 | 10 | 3 | 6 | 7 | 1 | 0 | 0 | 0 | 0 | 0 | 2 | 0 | 1 | 0 | 2 | 2 |
Bud Neck | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 1 |
Bud Site | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 2 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | – | – | – | – | – | – |
Cell Periphery | 0 | 0 | 0 | 0 | 2 | 0 | 4 | 1 | 0 | 1 | 2 | 1 | 0 | 2 | 4 | 3 | 5 | 2 | 0 | 0 | 0 | 0 | 0 | 0 |
Cytoplasm | 303 | 38 | 114 | 188 | 250 | 209 | 285 | 231 | 164 | 153 | 136 | 109 | 189 | 296 | 370 | 119 | 75 | 75 | 296 | 57 | 125 | 163 | 280 | 219 |
Endoplasmic Reticulum | 1 | 0 | 2 | 3 | 0 | 2 | 1 | 2 | 3 | 3 | 1 | 0 | 2 | 1 | 1 | 4 | 2 | 1 | 0 | 0 | 0 | 2 | 2 | 5 |
Endosome | 0 | 0 | 6 | 6 | 8 | 2 | 4 | 6 | 0 | 3 | 0 | 2 | 2 | 9 | 17 | 10 | 4 | 3 | 0 | 0 | 9 | 1 | 16 | 9 |
Golgi | 0 | 0 | 0 | 0 | 0 | 1 | 0 | 1 | 0 | 0 | 0 | 0 | 0 | 3 | 2 | 6 | 5 | 1 | 0 | 0 | 1 | 2 | 3 | 1 |
Mitochondria | 1 | 0 | 1 | 0 | 1 | 35 | 119 | 67 | 133 | 95 | 150 | 86 | 0 | 0 | 5 | 0 | 3 | 2 | 0 | 0 | 3 | 1 | 3 | 2 |
Nucleus | 1 | 0 | 4 | 5 | 2 | 6 | 14 | 11 | 12 | 6 | 11 | 11 | 2 | 3 | 1 | 4 | 0 | 1 | 8 | 1 | 2 | 7 | 10 | 9 |
Nuclear Periphery | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 0 | 0 | 0 | 1 | 0 | 0 | 2 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 1 |
Nucleolus | 0 | 0 | 0 | 2 | 0 | 0 | 5 | 0 | 0 | 1 | 1 | 1 | 0 | 2 | 5 | 4 | 2 | 2 | 0 | 0 | 0 | 0 | 0 | 1 |
Peroxisomes | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 2 | 0 | 0 | 0 | 1 | 0 | 0 | 0 | 0 | 0 |
SpindlePole | 0 | 0 | 0 | 1 | 2 | 0 | 0 | 3 | 1 | 0 | 0 | 1 | 2 | 0 | 5 | 1 | 4 | 0 | 0 | 0 | 1 | 0 | 6 | 5 |
Vac/Vac Membrane | 1 | 0 | 6 | 39 | 53 | 39 | 60 | 48 | 28 | 31 | 18 | 31 | 25 | 64 | 36 | 54 | 20 | 9 | 0 | 1 | 3 | 2 | 6 | 8 |
Unique Cell Count | 306 | 38 | 127 | 220 | 292 | 262 | 409 | 335 | 284 | 256 | 265 | 213 | 214 | 341 | 413 | 164 | 101 | 86 | 316 | 61 | 152 | 188 | 343 | 278 |
Labelled Cell Count | 307 | 38 | 133 | 245 | 319 | 297 | 492 | 375 | 353 | 296 | 325 | 251 | 223 | 380 | 450 | 206 | 120 | 96 | 316 | 61 | 152 | 188 | 343 | 278 |
Screen | WT1 | WT2 | WT3 | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 | AF100 | AF140 | AF180 |
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Mean Cell GFP Intensity (1e-4) | 7.8 | 10.7 | 8.2 | 8.7 | 8.0 | 6.0 | 5.5 | 5.7 | 4.9 | 5.1 | 4.7 | 5.0 | 9.8 | 9.4 | 8.5 | 14.9 | 16.1 | 15.4 | 9.6 | 9.9 | 9.8 |
Std Deviation (1e-4) | 1.3 | 2.0 | 1.5 | 1.4 | 1.5 | 1.5 | 1.3 | 1.3 | 1.1 | 1.2 | 1.4 | 1.3 | 1.6 | 1.6 | 1.3 | 3.7 | 3.1 | 3.8 | 1.7 | 1.9 | 1.7 |
Intensity Change (Log2) | – | – | – | 0.09 | -0.02 | -0.45 | -0.56 | -0.51 | -0.75 | -0.67 | -0.81 | -0.72 | 0.26 | 0.21 | 0.06 | 0.87 | 0.98 | 0.92 | 0.24 | 0.28 | 0.27 |
Localization | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 |
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Cortical Patches | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Bud | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Bud Neck | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Bud Site | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Cell Periphery | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Cytoplasm | -1.2 | -1.2 | -2.5 | -4.5 | -4.6 | -6.4 | -6.0 | -7.4 | -7.2 | -0.4 | -0.9 | -0.1 | -3.6 | -3.1 | -0.6 |
Endoplasmic Reticulum | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Endosome | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | -0.3 | 0.5 | 0 | 0 |
Golgi | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Mitochondria | 0 | 0 | 4.0 | 6.7 | 5.2 | 0 | 7.7 | 0 | 8.1 | 0 | 0 | 0 | 0 | 0 | 0 |
Nucleus | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0.9 | 0 | 0 | 0 | 0 | 0 | 0 |
Nuclear Periphery | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Nucleolus | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Peroxisomes | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
SpindlePole | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Vacuole | 3.5 | 3.6 | 2.9 | 3.0 | 2.9 | 1.7 | 2.3 | 0.8 | 2.8 | 2.2 | 3.8 | 1.5 | 5.9 | 3.6 | 1.6 |
R1 | R2 | R3 | ||||||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | |
Concentration | 4.3174 | 5.5881 | 5.004 | 4.591 | 4.6581 | 4.9598 | 4.2369 | 5.4271 | 4.9186 | 3.5825 | 4.8257 | 4.9981 | 5.4415 | 6.215 | 6.0536 | 5.8532 | 4.1121 | 6.062 |
Actin | 0.0127 | 0.0003 | 0.0036 | 0.0181 | 0.0021 | 0.0002 | 0.022 | 0.0008 | 0.0013 | 0.0024 | 0.0002 | 0.0124 | 0.0126 | 0.001 | 0.0052 | 0.0002 | 0.0318 | 0.0014 |
Bud | 0.0008 | 0.0005 | 0.0011 | 0.0019 | 0.0002 | 0.0001 | 0.0045 | 0.0078 | 0.0069 | 0.0004 | 0.0001 | 0.0004 | 0.0007 | 0.0004 | 0.0003 | 0.0001 | 0.0017 | 0.0002 |
Bud Neck | 0.002 | 0.0006 | 0.001 | 0.0008 | 0.0014 | 0.0017 | 0.0016 | 0.0006 | 0.0011 | 0.0015 | 0.0012 | 0.0035 | 0.0012 | 0.0005 | 0.0005 | 0.0006 | 0.0068 | 0.002 |
Bud Periphery | 0.0008 | 0.0001 | 0.0004 | 0.0029 | 0.0002 | 0 | 0.0015 | 0.0033 | 0.0014 | 0.0002 | 0 | 0.0004 | 0.0007 | 0.0001 | 0.0003 | 0 | 0.002 | 0.0001 |
Bud Site | 0.0011 | 0.0012 | 0.0017 | 0.0016 | 0.0004 | 0.0001 | 0.0064 | 0.0057 | 0.001 | 0.0005 | 0.0001 | 0.0007 | 0.002 | 0.0021 | 0.0007 | 0.0001 | 0.0023 | 0.0002 |
Cell Periphery | 0.0001 | 0 | 0.0001 | 0 | 0 | 0 | 0.0002 | 0.0001 | 0.0001 | 0 | 0 | 0 | 0.0001 | 0 | 0.0001 | 0 | 0.0003 | 0 |
Cytoplasm | 0.3514 | 0.4841 | 0.3845 | 0.219 | 0.3481 | 0.3396 | 0.4498 | 0.6184 | 0.4273 | 0.3818 | 0.5588 | 0.5504 | 0.4527 | 0.6525 | 0.4014 | 0.5197 | 0.2987 | 0.4289 |
Cytoplasmic Foci | 0.0183 | 0.0018 | 0.0078 | 0.0141 | 0.0077 | 0.0018 | 0.0364 | 0.005 | 0.0123 | 0.0246 | 0.0075 | 0.0069 | 0.0136 | 0.0043 | 0.0059 | 0.0011 | 0.046 | 0.0047 |
Eisosomes | 0.0002 | 0 | 0 | 0 | 0 | 0 | 0.0003 | 0 | 0 | 0 | 0 | 0.0001 | 0.0003 | 0 | 0.0001 | 0 | 0.0009 | 0 |
Endoplasmic Reticulum | 0.0087 | 0.0028 | 0.0046 | 0.0021 | 0.0044 | 0.002 | 0.0051 | 0.0028 | 0.0042 | 0.0026 | 0.0022 | 0.0046 | 0.0053 | 0.0026 | 0.0021 | 0.0011 | 0.0203 | 0.0028 |
Endosome | 0.0436 | 0.0031 | 0.0113 | 0.013 | 0.0221 | 0.0037 | 0.0357 | 0.0038 | 0.01 | 0.0206 | 0.0041 | 0.0094 | 0.0188 | 0.0034 | 0.0039 | 0.0008 | 0.078 | 0.0069 |
Golgi | 0.0052 | 0.0001 | 0.0026 | 0.003 | 0.0033 | 0.0001 | 0.0072 | 0.0003 | 0.0007 | 0.0069 | 0.0002 | 0.0019 | 0.0038 | 0.0005 | 0.0014 | 0 | 0.0204 | 0.0011 |
Lipid Particles | 0.0124 | 0 | 0.0027 | 0.0019 | 0.0145 | 0 | 0.0163 | 0.0001 | 0.0005 | 0.0737 | 0 | 0.001 | 0.0065 | 0.0002 | 0.0039 | 0 | 0.0633 | 0.0024 |
Mitochondria | 0.0055 | 0.0005 | 0.0037 | 0.0038 | 0.0076 | 0.0004 | 0.0066 | 0.0074 | 0.0012 | 0.0021 | 0.0003 | 0.0133 | 0.0085 | 0.0093 | 0.012 | 0.0003 | 0.0339 | 0.0016 |
None | 0.0137 | 0.0037 | 0.0077 | 0.0022 | 0.0103 | 0.0044 | 0.0341 | 0.0148 | 0.0143 | 0.0063 | 0.0092 | 0.0035 | 0.0141 | 0.0049 | 0.0091 | 0.0009 | 0.0133 | 0.0011 |
Nuclear Periphery | 0.031 | 0.0197 | 0.0195 | 0.0138 | 0.0487 | 0.0159 | 0.032 | 0.0145 | 0.0272 | 0.015 | 0.0109 | 0.0204 | 0.0253 | 0.0137 | 0.0155 | 0.0114 | 0.0706 | 0.0187 |
Nucleolus | 0.0012 | 0.0005 | 0.0011 | 0.0008 | 0.0016 | 0.0005 | 0.0013 | 0.0005 | 0.0005 | 0.0008 | 0.0003 | 0.0005 | 0.0015 | 0.0003 | 0.0003 | 0.0002 | 0.0023 | 0.0004 |
Nucleus | 0.4558 | 0.4705 | 0.5268 | 0.6668 | 0.4673 | 0.6186 | 0.3107 | 0.2987 | 0.4777 | 0.4179 | 0.3973 | 0.3601 | 0.4094 | 0.296 | 0.5268 | 0.4606 | 0.2535 | 0.5149 |
Peroxisomes | 0.0087 | 0.0001 | 0.0015 | 0.0178 | 0.0065 | 0.0001 | 0.0141 | 0.0002 | 0.001 | 0.0293 | 0.0001 | 0.0014 | 0.0087 | 0.0007 | 0.0054 | 0 | 0.0241 | 0.0017 |
Punctate Nuclear | 0.0201 | 0.0073 | 0.0146 | 0.0041 | 0.045 | 0.0083 | 0.0095 | 0.0128 | 0.0079 | 0.0115 | 0.0067 | 0.0055 | 0.0069 | 0.0056 | 0.0035 | 0.0019 | 0.0148 | 0.0088 |
Vacuole | 0.0049 | 0.0027 | 0.0029 | 0.0107 | 0.0043 | 0.002 | 0.0031 | 0.0019 | 0.0021 | 0.0015 | 0.0006 | 0.0026 | 0.0058 | 0.0015 | 0.0012 | 0.0008 | 0.0095 | 0.0016 |
Vacuole Periphery | 0.0019 | 0.0005 | 0.0008 | 0.0016 | 0.0041 | 0.0004 | 0.0016 | 0.0004 | 0.0013 | 0.0006 | 0.0002 | 0.0008 | 0.0015 | 0.0004 | 0.0004 | 0.0002 | 0.0056 | 0.0005 |
R1 | R2 | |||||||||
---|---|---|---|---|---|---|---|---|---|---|
G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | |
Gene Expression | 94.1267 | 60.311 | 56.3026 | 63.6434 | 65.605 | 47.853 | 53.1119 | 49.7875 | 45.5668 | 72.5471 |
Translational Efficiency | 0.9182 | 1.3455 | 1.2034 | 0.9147 | 1.0918 | 1.4382 | 1.4176 | 1.0662 | 1.2503 | 1.1427 |
Dataset | Hit |
---|---|
Protein Concentration | ✘ |
Protein Localization | ✘ |
Gene Expression | ✘ |
Translational Efficiency | ✘ |
R1 | R2 | R1 & R2 | |||||||||
---|---|---|---|---|---|---|---|---|---|---|---|
WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 |
1938 | 368 | 2671 | 1492 | 1877 | 1678 | 145 | 1352 | 3815 | 2046 | 2816 | 2844 |
R1 | R2 | R1 & R2 | ||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|
WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 | |
Mean | 957.56 | 996.44 | 1294.52 | 1413.20 | 965.87 | 1079.01 | 1456.30 | 1447.23 | 961.65 | 1064.16 | 1302.85 | 1429.38 |
Standard Deviation | 143.62 | 198.53 | 193.49 | 232.85 | 147.60 | 176.64 | 196.56 | 243.17 | 145.65 | 183.53 | 196.92 | 238.42 |
Intensity Change Log 2 | — | 0.057420 | 0.434982 | 0.561531 | — | 0.159807 | 0.592407 | 0.583393 | — | 0.109743 | 0.516180 | 0.572551 |
R1 | R2 | R1 & R2 | ||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|
WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 | |
Actin | 0.000204 | 0.001082 | 0.000224 | 0.001040 | 0.000245 | 0.000467 | 0.001130 | 0.009038 | 0.000224 | 0.000577 | 0.000270 | 0.004842 |
Bud Neck | 0.003626 | 0.004767 | 0.002591 | 0.004490 | 0.002592 | 0.003981 | 0.004605 | 0.007673 | 0.003117 | 0.004122 | 0.002694 | 0.006003 |
Bud Site | 0.004661 | 0.017547 | 0.002052 | 0.012587 | 0.003408 | 0.012561 | 0.008333 | 0.040394 | 0.004044 | 0.013457 | 0.002376 | 0.025806 |
Cell Periphery | 0.000076 | 0.000140 | 0.000058 | 0.000176 | 0.000082 | 0.000092 | 0.000262 | 0.000128 | 0.000079 | 0.000101 | 0.000068 | 0.000153 |
Cytoplasm | 0.672374* | 0.673369* | 0.487510* | 0.513769* | 0.680494* | 0.702634* | 0.403649* | 0.384217* | 0.676369* | 0.697370* | 0.483192* | 0.452182* |
Cytoplasmic Foci | 0.108047 | 0.066585 | 0.001406 | 0.014197 | 0.051280 | 0.058524 | 0.012081 | 0.048007 | 0.080117 | 0.059974 | 0.001955 | 0.030270 |
Eisosomes | 0.000026 | 0.000029 | 0.000004 | 0.000012 | 0.000017 | 0.000018 | 0.000033 | 0.000015 | 0.000022 | 0.000020 | 0.000006 | 0.000013 |
Endoplasmic Reticulum | 0.002641 | 0.002539 | 0.000659 | 0.000847 | 0.002975 | 0.001620 | 0.003986 | 0.000446 | 0.002805 | 0.001785 | 0.000830 | 0.000656 |
Endosome | 0.004642 | 0.009841 | 0.000193 | 0.007457 | 0.002401 | 0.005470 | 0.003462 | 0.014287 | 0.003540 | 0.006256 | 0.000362 | 0.010704 |
Golgi | 0.002103 | 0.001772 | 0.000047 | 0.007226 | 0.000655 | 0.001827 | 0.000365 | 0.029410 | 0.001390 | 0.001817 | 0.000063 | 0.017772 |
Lipid Particles | 0.002060 | 0.001836 | 0.000231 | 0.001379 | 0.001051 | 0.001014 | 0.002699 | 0.002315 | 0.001563 | 0.001162 | 0.000358 | 0.001824 |
Mitochondria | 0.002156 | 0.000501 | 0.000753 | 0.004363 | 0.002268 | 0.001518 | 0.000513 | 0.004836 | 0.002211 | 0.001335 | 0.000741 | 0.004588 |
Mitotic Spindle | 0.002060 | 0.006862 | 0.007599 | 0.147076* | 0.004230 | 0.011009 | 0.032134 | 0.301437* | 0.003127 | 0.010263 | 0.008862 | 0.220457* |
None | 0.003294 | 0.008813 | 0.009072 | 0.006995 | 0.003629 | 0.004093 | 0.005150 | 0.005800 | 0.003459 | 0.004942 | 0.008870 | 0.006427 |
Nuclear Periphery | 0.000353 | 0.000306 | 0.000915 | 0.000839 | 0.000462 | 0.000430 | 0.002094 | 0.000614 | 0.000406 | 0.000408 | 0.000975 | 0.000732 |
Nuclear Periphery Foci | 0.002012 | 0.004980 | 0.000370 | 0.005107 | 0.002784 | 0.002778 | 0.006931 | 0.003573 | 0.002392 | 0.003174 | 0.000708 | 0.004378 |
Nucleolus | 0.000493 | 0.001823 | 0.000120 | 0.000556 | 0.000401 | 0.000431 | 0.000264 | 0.000632 | 0.000448 | 0.000682 | 0.000128 | 0.000592 |
Nucleus | 0.140238* | 0.126859 | 0.472008* | 0.221696* | 0.209543* | 0.130606* | 0.341236* | 0.101888 | 0.174336* | 0.129932 | 0.465274* | 0.164741* |
Peroxisomes | 0.001245 | 0.003120 | 0.000020 | 0.000553 | 0.000634 | 0.001593 | 0.000293 | 0.004434 | 0.000944 | 0.001868 | 0.000034 | 0.002398 |
Vacuole | 0.046685 | 0.065179 | 0.014093 | 0.045147 | 0.030397 | 0.058663 | 0.170522 | 0.035489 | 0.038671 | 0.059835 | 0.022147 | 0.040555 |
Vacuole Periphery | 0.001004 | 0.002049 | 0.000075 | 0.004488 | 0.000454 | 0.000672 | 0.000258 | 0.005369 | 0.000734 | 0.000919 | 0.000085 | 0.004907 |
R1 | R2 | R1 & R2 | |||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
UBP2_WT | UBP14_WT | UBP2UBP14_WT | UBP2UBP14_UBP2 | UBP2UBP14_UBP14 | UBP2_WT | UBP14_WT | UBP2UBP14_WT | UBP2UBP14_UBP2 | UBP2UBP14_UBP14 | UBP2_WT | UBP14_WT | UBP2UBP14_WT | UBP2UBP14_UBP2 | UBP2UBP14_UBP14 | |
Actin | -3.17 | -0.90 | -5.88 | 1.27 | -4.63 | -3.38 | -8.22 | -2.25 | -1.94 | -0.50 | -5.11 | -1.70 | -3.00 | -1.95 | -2.71 |
Bud Neck | -1.41 | 0.62 | -7.23 | -3.09 | -7.86 | -2.62 | -5.81 | -9.46 | -7.81 | -1.03 | -2.55 | -1.08 | -11.81 | -8.65 | -10.26 |
Bud Site | -5.42 | 4.59 | -8.88 | 1.05 | -10.74 | -6.60 | -6.73 | -12.98 | -7.48 | -8.81 | -7.99 | 2.75 | -15.63 | -5.59 | -16.50 |
Cell Periphery | -4.29 | 1.54 | -7.80 | -1.95 | -8.19 | -1.34 | -9.40 | -7.46 | -5.00 | 7.11 | -3.39 | -1.07 | -10.35 | -6.12 | -9.27 |
Cytoplasm | 1.55 | 34.40 | 28.89 | 14.71 | 1.47 | -0.16 | 25.00 | 31.50 | 29.92 | -7.22 | 0.14 | 43.88 | 42.67 | 35.70 | 3.24 |
Cytoplasmic Foci | 5.77 | 31.07 | 19.67 | 5.88 | -11.62 | -2.07 | 18.61 | 0.96 | 2.65 | -12.80 | 6.49 | 37.59 | 15.43 | 7.53 | -19.12 |
Eisosomes | -1.41 | 15.16 | 9.03 | 4.57 | -7.51 | -1.73 | -9.40 | 2.85 | 5.30 | 10.69 | -0.35 | 12.58 | 8.39 | 7.22 | -5.30 |
Endoplasmic Reticulum | -0.14 | 6.77 | 7.63 | 4.48 | 2.92 | 3.53 | -4.15 | 8.09 | 9.42 | 9.01 | 3.61 | 7.62 | 11.03 | 9.37 | 9.40 |
Endosome | -2.41 | 11.02 | 3.18 | 3.45 | -6.47 | -5.30 | -3.44 | -3.75 | 0.25 | -0.91 | -4.42 | 12.17 | -0.87 | 3.28 | -9.10 |
Golgi | 0.94 | 5.22 | 0.65 | -0.19 | -3.11 | -2.42 | 2.33 | -3.13 | -2.43 | -3.32 | -0.57 | 6.35 | -2.59 | -2.21 | -4.37 |
Lipid Particles | 0.21 | 6.99 | -6.34 | -5.20 | -14.43 | -0.24 | -8.04 | -10.32 | -10.22 | 0.21 | 2.06 | 7.04 | -11.64 | -13.77 | -17.81 |
Mitochondria | 3.03 | 2.23 | 1.15 | -2.39 | -1.23 | 1.00 | 1.94 | 1.12 | 0.21 | -0.86 | 1.68 | 2.76 | 1.58 | -0.21 | -1.26 |
Mitotic Spindle | -1.85 | -5.58 | -20.41 | -19.05 | -19.31 | -3.03 | -2.14 | -26.06* | -25.19* | -20.85* | -4.08 | -5.12 | -32.69 | -30.92* | -31.23* |
None | -1.84 | -17.21 | -9.19 | 0.02 | 2.76 | -0.38 | -0.44 | -6.65 | -4.60 | -3.59 | -1.68 | -18.68 | -11.17 | -4.14 | 3.34 |
Nuclear Periphery | -0.36 | -22.73 | -18.41 | -17.93 | -4.28 | -0.58 | -10.40 | -13.30 | -14.15 | 7.39 | -1.92 | -24.50 | -22.55 | -21.74 | -0.25 |
Nuclear Periphery Foci | -3.86 | 9.43 | -8.30 | -2.63 | -10.23 | -0.75 | -4.45 | -5.19 | -4.77 | 1.56 | -3.51 | 7.64 | -9.67 | -6.83 | -12.84 |
Nucleolus | -2.39 | 4.15 | -4.57 | 0.89 | -7.88 | -0.29 | 0.24 | -3.92 | -5.57 | -6.30 | -1.72 | 3.47 | -5.96 | -3.88 | -11.35 |
Nucleus | 0.51 | -62.64 | -11.54 | -7.65 | 40.80 | 12.23 | -6.44 | 13.21 | 2.01 | 10.19 | 7.94 | -59.64 | 0.45 | -6.62 | 54.86 |
Peroxisomes | -2.41 | 7.42 | 4.26 | 3.38 | -7.96 | -3.43 | 2.68 | -4.54 | -1.83 | -6.17 | -3.49 | 8.50 | -1.90 | 1.91 | -8.38 |
Vacuole | -5.11 | 4.88 | -15.73 | -5.51 | -18.58 | -11.98 | -12.81 | -16.80 | -7.75 | 8.82 | -11.55 | -3.91 | -23.03 | -10.86 | -17.90 |
Vacuole Periphery | -1.26 | 4.70 | 0.01 | 1.17 | -2.86 | -1.38 | 1.55 | -1.68 | -1.23 | -2.22 | -0.62 | 5.58 | -1.17 | -0.78 | -3.92 |
Temp | Actin Patch (Sac6-tdTomato) | Cortical Patch (Sla1-GFP) | Late Endosome (Snf7-GFP) | Vacuole (Vph1-GFP) |
---|---|---|---|---|
37℃ | ||||
RT |
Gene / Allele | Actin Patch (Sac6-tdTomato) | Cortical Patch (Sla1-GFP) | Late Endosome (Snf7-GFP) | Vacuole (Sac6-tdTomato) |
---|
Gene | Images |
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Gene | Images |
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