Standard name
Human Ortholog
Description Subunit of the transmembrane ring of the nuclear pore complex (NPC); contributes to nucleocytoplasmic transport, NPC biogenesis and spindle pole body duplication
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Micrographs

ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 – – – – – –
Cell Periphery 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cytoplasm 0 0 0 0 0.06 0 0 0 – 0 0 0 0 0.09 0.12 0.06 0.06 0 0 0 0 0 0 0
Endoplasmic Reticulum 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Endosome 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0.07
Golgi 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Mitochondria 0 0 0.2 0.08 0.22 0.53 0.47 0.52 – 0.48 0.61 0.56 0 0 0 0 0.12 0 0 0 0 0 0.07 0.12
Nucleus 0.07 0 0.05 0.12 0.1 0.13 0.09 0.12 – 0.09 0.07 0.14 0.07 0.05 0 0.06 0 0 0 0 0 0 0 0
Nuclear Periphery 0.86 0.8 0.87 0.78 0.58 0.54 0.52 0.44 – 0.48 0.52 0.48 0.81 0.79 0.74 0.61 0.74 0.8 0.9 0.86 0.92 0.86 0.78 0.65
Nucleolus 0.17 0 0.07 0 0.07 0.1 0.12 0.16 – 0.18 0.2 0.15 0 0.06 0.07 0.09 0.12 0.12 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0.06 0 0 0 0 0 0 0 0 0
Vac/Vac Membrane 0 0.31 0.11 0.22 0.22 0.08 0.07 0.07 – 0.11 0.07 0.05 0.25 0.17 0.16 0.37 0.29 0.36 0 0.06 0 0 0.06 0.06
ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 1 0 0 0 1
Bud 1 0 0 1 1 1 1 3 – 3 1 3 0 0 0 0 0 0 3 0 0 1 0 3
Bud Neck 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 1 6 1 6 – 10 2 11 0 3 1 0 0 0 – – – – – –
Cell Periphery 0 2 0 0 0 1 0 0 – 1 0 0 0 1 1 0 0 0 0 0 0 0 0 0
Cytoplasm 5 6 3 1 12 5 10 18 – 12 10 8 5 22 25 8 9 1 0 0 0 0 1 0
Endoplasmic Reticulum 0 3 0 0 0 1 0 0 – 0 2 0 2 2 3 3 1 1 0 0 0 1 1 2
Endosome 0 0 3 0 5 2 6 3 – 10 2 4 1 2 3 2 2 1 2 3 2 1 6 13
Golgi 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 1 0 0 0 0 0 0 1 3
Mitochondria 4 4 51 14 45 163 207 232 – 216 283 288 1 2 1 3 19 6 0 6 6 4 15 24
Nucleus 10 10 13 22 21 39 41 53 – 42 34 73 14 13 6 8 2 3 2 3 1 1 1 1
Nuclear Periphery 124 197 216 137 120 166 231 196 – 219 242 243 171 201 160 78 119 109 154 241 225 150 167 136
Nucleolus 25 9 18 7 15 31 54 69 – 82 93 79 6 16 16 11 20 17 4 6 2 2 2 2
Peroxisomes 0 0 0 0 0 1 1 0 – 1 0 2 0 1 1 1 0 0 0 0 0 0 0 3
SpindlePole 0 0 0 0 1 1 4 3 – 2 1 1 1 4 13 1 0 0 0 0 1 0 0 2
Vac/Vac Membrane 4 76 28 39 46 25 31 29 – 50 34 25 53 43 35 47 46 49 2 16 3 6 13 12
Unique Cell Count 144 245 249 176 208 307 444 444 452 465 511 211 254 217 127 161 137 172 282 246 174 215 210
Labelled Cell Count 173 307 332 221 267 442 587 612 648 704 737 254 310 265 163 218 187 172 282 246 174 215 210


Nuclear Periphery

(Huh et al. Nature 2003 425:686)
Screen WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 AF100 AF140 AF180
Mean Cell GFP Intensity (1e-4) 10.2 15.5 8.8 9.5 8.0 6.1 5.9 5.9 – 5.9 5.6 5.7 12.4 11.2 11.5 14.3 13.6 15.9 13.4 14.0 13.8
Std Deviation (1e-4) 2.1 4.6 2.0 2.0 5.1 1.6 1.5 1.5 – 2.2 1.6 1.5 2.8 3.2 3.8 4.6 4.2 3.4 3.2 3.1 3.6
Intensity Change (Log2) – – – 0.11 -0.13 -0.54 -0.59 -0.57 – -0.57 -0.66 -0.62 0.5 0.35 0.39 0.71 0.63 0.86 0.61 0.67 0.66


Localization RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3
Cortical Patches 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0
Bud 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0
Cell Periphery 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0
Cytoplasm 0 2.7 0 0 2.1 – 1.3 0 0 0 3.8 4.7 0 0 0
Endoplasmic Reticulum 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0
Endosome 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0
Golgi 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0
Mitochondria -3.5 0.3 7.9 6.8 8.2 – 7.1 10.3 9.3 -6.8 -7.2 -6.8 -4.7 -2.3 -4.3
Nucleus 2.7 2.0 3.0 1.9 2.9 – 1.9 1.1 3.7 0.6 -0.1 -1.3 0.4 -2.1 -1.4
Nuclear Periphery -2.4 -7.0 -8.3 -9.2 -11.0 – -10.0 -9.2 -10.4 -1.7 -2.3 -3.5 -5.6 -3.3 -1.9
Nucleolus -1.4 0 1.2 2.0 3.2 – 4.0 4.5 3.2 -2.1 -0.4 0.1 0.5 1.8 1.7
Peroxisomes 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 – 0 0 0 0 0 3.9 0 0 0
Vacuole 3.0 3.1 -1.2 -1.9 -2.2 – -0.1 -1.8 -3.2 3.9 1.8 1.5 5.9 4.5 5.8

Images

Circle plot not available

Protein Concentration and Protein Localization Data

R1 R2 R3
G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase
Concentration 9.1986 8.1678 8.0352 7.6307 7.6385 7.6181 11.4477 10.7588 10.3147 9.9722 9.1617 10.2443 9.4048 7.5665 7.8736 7.1722 7.2488 7.3456
Actin 0.0058 0.0001 0.0001 0.0004 0.0002 0 0.0123 0 0.0018 0.0258 0.0002 0.0017 0.0004 0.0001 0.0001 0.0003 0.0003 0
Bud 0 0 0 0 0 0 0.0001 0 0.0001 0.0001 0 0 0 0 0 0 0 0
Bud Neck 0.0001 0 0.0001 0 0 0 0.0004 0 0.0005 0 0 0 0 0 0 0 0.0002 0
Bud Periphery 0.0001 0 0.0002 0.0001 0.0001 0 0.0003 0 0.0002 0.0001 0 0 0.0001 0 0 0.0001 0.0002 0
Bud Site 0.0001 0 0.0001 0 0 0 0.004 0 0.0017 0.0003 0 0 0 0 0 0 0 0
Cell Periphery 0 0 0 0.0001 0 0 0.0001 0 0.0001 0 0 0 0 0 0 0 0 0
Cytoplasm 0.0042 0.0001 0.0001 0.0001 0 0 0.0015 0 0.0007 0.0002 0 0.0001 0.0001 0.0004 0.0001 0 0.0001 0
Cytoplasmic Foci 0.0009 0 0 0.0002 0.0001 0 0.0032 0 0.0015 0.008 0.0009 0.0003 0.0018 0 0 0.0001 0.0002 0
Eisosomes 0 0 0 0 0 0 0.0002 0 0.0002 0 0 0 0 0 0 0 0 0
Endoplasmic Reticulum 0.0007 0.0004 0.0006 0.0016 0.0003 0.0002 0.0035 0.0004 0.0008 0.0005 0.0006 0.0004 0.0004 0.0005 0.0005 0.0011 0.0007 0.0001
Endosome 0.0005 0.0002 0.0003 0.0009 0.0006 0.0001 0.0116 0.0001 0.0016 0.0018 0.0016 0.0006 0.0003 0.0002 0.0002 0.0004 0.001 0.0001
Golgi 0.0002 0 0 0.0006 0.0004 0 0.0031 0 0.0006 0.0042 0.0012 0.0004 0.0002 0.0001 0 0.0003 0.0009 0
Lipid Particles 0.0011 0.0002 0.0004 0.0103 0.0047 0.0004 0.0152 0.0007 0.0033 0.0035 0.0279 0.0006 0.0015 0.001 0.0003 0.0023 0.0137 0.0004
Mitochondria 0.0006 0.0002 0.0007 0.0016 0.0007 0.0003 0.0022 0.0002 0.0021 0.001 0.001 0.0002 0.0011 0.0002 0.0006 0.0014 0.0013 0.0001
None 0.0005 0 0.0003 0.0001 0.0001 0 0.0011 0 0.0072 0.0003 0.0002 0 0.0003 0.0001 0.0001 0.0001 0.0002 0
Nuclear Periphery 0.9359 0.8921 0.9217 0.9343 0.9463 0.9713 0.9007 0.9646 0.9305 0.905 0.9282 0.9827 0.9464 0.9188 0.9414 0.9224 0.9202 0.9799
Nucleolus 0.0003 0.0029 0.0022 0.002 0.0018 0.0021 0.0004 0.0001 0.0012 0.0003 0.0007 0.0001 0.0004 0.0011 0.0003 0.0006 0.0033 0.0002
Nucleus 0.0201 0.0729 0.0437 0.0183 0.0186 0.0109 0.0053 0.0023 0.0041 0.0019 0.0016 0.0006 0.0146 0.0526 0.0203 0.0367 0.0264 0.0074
Peroxisomes 0.0013 0 0 0.0001 0.0001 0 0.0014 0 0.0006 0.0128 0.0001 0.0001 0.0011 0 0 0.0001 0.0002 0
Punctate Nuclear 0.0006 0.0039 0.0005 0.0011 0.0018 0.0005 0.003 0.0003 0.001 0.0009 0.0013 0.0004 0.0014 0.0006 0.0003 0.001 0.0018 0.0003
Vacuole 0.0004 0.0011 0.0018 0.001 0.0004 0.0002 0.0012 0.0002 0.0011 0.0003 0.0007 0 0.0003 0.0006 0.0003 0.0005 0.0015 0.0001
Vacuole Periphery 0.0267 0.0257 0.0271 0.0271 0.0239 0.0139 0.0293 0.031 0.039 0.0328 0.0336 0.0117 0.0296 0.0237 0.0355 0.0327 0.0277 0.0114

Sequencing Data

R1 R2
G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Post-START S/G2 Metaphase Anaphase Telophase
Gene Expression 44.1362 36.1671 30.0906 35.6321 31.7914 25.7319 34.9063 34.4042 31.8182 35.6538
Translational Efficiency 0.9538 0.9171 1.1754 0.8266 1.2198 1.4705 0.8165 1.0302 1.0215 1.1833

Hit Data

Dataset Hit
Protein Concentration ✘
Protein Localization ✘
Gene Expression ✘
Translational Efficiency ✘

Micrographs

Cell Count

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
1740 2340 1840 1443 2348 1520 2179 1439 4088 3860 4019 2882

Protein Abundance

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
Mean 1094.24 1247.91 1354.74 1303.95 1142.48 1122.05 1415.84 1414.30 1121.95 1198.35 1387.87 1359.05
Standard Deviation 195.43 239.26 213.71 230.71 196.61 208.99 212.75 231.54 197.55 235.98 215.35 237.62
Intensity Change Log 2 — 0.189585 0.308087 0.252959 — -0.026032 0.309489 0.307919 — 0.083478 0.308803 0.281293

Localization Score (DeepLoc)

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
Actin 0.000360 0.000345 0.000319 0.000561 0.000448 0.000418 0.000246 0.000256 0.000410 0.000374 0.000280 0.000409
Bud Neck 0.000298 0.000249 0.000727 0.004221 0.000460 0.000308 0.000300 0.000940 0.000391 0.000272 0.000495 0.002583
Bud Site 0.000090 0.000062 0.000044 0.001307 0.000130 0.000079 0.000074 0.000198 0.000113 0.000069 0.000060 0.000753
Cell Periphery 0.000040 0.000031 0.000018 0.000098 0.000047 0.000043 0.000012 0.000039 0.000044 0.000036 0.000015 0.000069
Cytoplasm 0.000182 0.000236 0.000151 0.002963 0.000273 0.000896 0.000066 0.000687 0.000234 0.000496 0.000104 0.001826
Cytoplasmic Foci 0.000399 0.000409 0.000108 0.003735 0.000366 0.000720 0.000088 0.001769 0.000380 0.000531 0.000097 0.002753
Eisosomes 0.000006 0.000006 0.000003 0.000012 0.000009 0.000006 0.000002 0.000004 0.000008 0.000006 0.000003 0.000008
Endoplasmic Reticulum 0.004344 0.001844 0.001038 0.002352 0.003500 0.004287 0.000566 0.002066 0.003859 0.002806 0.000782 0.002209
Endosome 0.000665 0.000588 0.000186 0.004280 0.000887 0.000969 0.000070 0.001505 0.000792 0.000738 0.000123 0.002894
Golgi 0.000197 0.000277 0.000099 0.002111 0.000280 0.000392 0.000066 0.001855 0.000245 0.000322 0.000081 0.001983
Lipid Particles 0.002524 0.000907 0.000111 0.004076 0.002966 0.002628 0.000097 0.005071 0.002778 0.001585 0.000104 0.004573
Mitochondria 0.004244 0.002504 0.001629 0.004606 0.005399 0.005794 0.001411 0.005119 0.004907 0.003800 0.001510 0.004862
Mitotic Spindle 0.000528 0.000741 0.003654 0.077021 0.001411 0.003902 0.000998 0.009766 0.001035 0.001986 0.002214 0.043440
None 0.000653 0.000491 0.000244 0.001701 0.001022 0.000726 0.000111 0.000275 0.000865 0.000584 0.000172 0.000989
Nuclear Periphery 0.671381* 0.733418* 0.744451* 0.523634* 0.660576* 0.678603* 0.794375* 0.657288* 0.665175* 0.711833* 0.771519* 0.590369*
Nuclear Periphery Foci 0.261166* 0.225147* 0.189968 0.285395* 0.266298* 0.248650* 0.173241 0.280337* 0.264114* 0.234402* 0.180899 0.282869*
Nucleolus 0.020327 0.010623 0.025735 0.026050 0.020892 0.018249 0.011952 0.004141 0.020651 0.013626 0.018262 0.015111
Nucleus 0.015709 0.009838 0.025729 0.031445 0.019393 0.020974 0.007946 0.003967 0.017825 0.014223 0.016088 0.017725
Peroxisomes 0.000527 0.000395 0.000405 0.000700 0.000536 0.000533 0.000238 0.000387 0.000532 0.000449 0.000314 0.000544
Vacuole 0.003856 0.002252 0.000772 0.010571 0.004270 0.003595 0.000933 0.006439 0.004094 0.002781 0.000859 0.008508
Vacuole Periphery 0.012505 0.009638 0.004610 0.013162 0.010836 0.008226 0.007208 0.017891 0.011546 0.009082 0.006019 0.015523

Localization Changes (T-score)

R1 R2 R1 & R2
UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14 UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14 UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14
Actin 0.32 0.88 -4.30 -4.35 -4.92 0.54 2.88 2.98 2.40 -0.21 1.01 3.04 -1.08 -1.82 -3.38
Bud Neck 0.98 -5.15 -10.57 -10.68 -9.21 1.71 1.72 -3.74 -6.17 -6.14 2.12 -2.18 -10.95 -11.67 -10.34
Bud Site 0.55 1.00 -5.27 -5.38 -5.45 0.78 0.67 -0.94 -1.98 -1.63 1.01 1.00 -5.27 -5.67 -5.64
Cell Periphery 1.03 2.82 -5.54 -6.85 -8.50 0.54 5.64 1.15 0.41 -5.45 1.42 6.03 -4.12 -5.51 -10.02
Cytoplasm -0.53 0.31 -4.23 -4.13 -4.27 -1.15 2.53 -1.07 -0.05 -1.40 -1.18 2.20 -3.90 -3.05 -4.17
Cytoplasmic Foci -0.08 3.57 -4.63 -4.55 -5.05 -2.29 5.05 -3.59 -2.59 -4.33 -1.32 6.04 -5.79 -5.30 -6.50
Eisosomes 0.63 3.91 -3.46 -3.83 -5.22 2.90 8.98 4.93 2.28 -3.61 2.86 9.48 -0.81 -2.65 -6.12
Endoplasmic Reticulum 5.99 8.69 4.22 -2.52 -6.95 -1.82 10.99 3.69 4.95 -5.35 3.73 13.79 5.53 1.99 -8.58
Endosome 0.35 4.21 -6.19 -6.20 -7.09 -0.39 6.69 -2.01 -1.75 -5.08 0.40 7.85 -6.35 -6.38 -8.58
Golgi -0.50 1.77 -5.01 -4.49 -5.31 -0.58 1.64 -2.24 -2.10 -2.60 -0.57 2.07 -4.36 -4.12 -4.88
Lipid Particles 3.24 5.06 -1.36 -2.99 -3.77 0.41 4.70 -1.86 -2.20 -5.20 2.53 6.60 -2.21 -3.99 -6.29
Mitochondria 2.60 3.83 -0.78 -3.94 -5.30 -0.36 5.80 0.22 0.49 -3.96 1.85 6.95 -0.13 -1.81 -6.19
Mitotic Spindle -0.78 -4.77 -14.96 -14.91 -14.20 -1.94 1.02 -4.84 -2.93 -5.13 -1.72 -3.23 -15.28 -14.80 -14.76
None 1.08 2.75 -2.52 -2.86 -3.38 1.10 4.07 3.30 2.73 -3.82 1.74 4.79 -0.69 -1.91 -3.75
Nuclear Periphery -9.35 -9.53 16.53 24.72* 24.13 -2.37 -22.93 0.54 2.60 19.66 -9.59 -22.63 12.98 20.63 30.80
Nuclear Periphery Foci 6.80 13.19 -2.60 -8.17 -13.27 2.97 19.86* -2.19 -4.62 -18.69* 7.67 23.45 -3.33 -9.72 -22.40
Nucleolus 5.01 -2.52 -2.98 -6.97 -0.81 1.17 4.93 10.42 7.22 5.69 4.97 1.24 2.25 -1.96 1.15
Nucleus 2.87 -4.04 -6.33 -8.52 -2.75 -0.57 5.86 8.04 6.83 3.08 2.14 0.62 -1.48 -3.29 -2.01
Peroxisomes 2.68 2.51 -1.75 -2.61 -2.54 0.06 7.85 0.38 0.37 -0.75 2.18 7.24 -0.55 -1.08 -1.98
Vacuole 3.29 7.18 -6.00 -7.42 -8.70 0.83 7.43 -2.48 -2.78 -7.09 2.99 10.23 -6.30 -7.91 -11.09
Vacuole Periphery 2.36 7.05 -0.31 -2.28 -5.97 2.25 3.78 -4.50 -5.90 -7.11 3.05 7.64 -3.42 -5.82 -9.11
Standard name
Human Ortholog
Description Subunit of the transmembrane ring of the nuclear pore complex (NPC); contributes to nucleocytoplasmic transport, NPC biogenesis and spindle pole body duplication
Localization
Cell Percentages nuclear periphery (98%)
Cell Cycle Regulation No
Subcompartmental Group N/A

Pom34

Pom34


Nearest neighbours ( genes)

correlation threshold

0.6

1.0

0.80


Biological Process (Gene Ontology)
Molecular Function (Gene Ontology)
Cellular Component (Gene Ontology)

Endocytosis
Temp Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Vph1-GFP)
37℃
RT
Cell Cycle Omics

CYCLoPs (Pom34-GFP)
Gene / Allele Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Sac6-tdTomato)
Gene Images
Gene Images
Images are not yet available
Images are not yet available