Standard name
Human Ortholog
Description Enzyme of 'de novo' purine biosynthesis; contains both 5-aminoimidazole-4-carboxamide ribonucleotide transformylase and inosine monophosphate cyclohydrolase activities; ADE16 has a paralog, ADE17, that arose from the whole genome duplication; ade16 ade17 mutants require adenine and histidine
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Micrographs

ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 – – 0 0 0 – 0 0 0 0 – – 0 0 0 0 0 0 0 0 0 0
Bud 0 0 – – 0 0 0 – 0 0 0 0 – – 0 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 – – 0 0 0 – 0 0 0 0 – – 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 – – 0 0 0 – 0 0 0 0 – – 0 0 0 0 – – – – – –
Cell Periphery 0 0 – – 0 0.05 0.05 – 0 0.09 0.06 0.09 – – 0.07 0.1 0.1 0.11 0 0 0 0 0 0
Cytoplasm 0.97 1.0 – – 0.99 0.98 0.95 – 0.93 0.86 0.79 0.86 – – 1.0 0.94 0.98 0.97 0.93 0.96 0.92 0.85 0.91 0.8
Endoplasmic Reticulum 0 0 – – 0 0 0 – 0 0.07 0.09 0.05 – – 0 0 0.05 0 0 0 0 0 0 0
Endosome 0 0 – – 0 0 0 – 0 0 0 0 – – 0 0 0 0 0 0 0 0 0 0
Golgi 0 0 – – 0 0 0 – 0 0 0 0 – – 0 0 0 0 0 0 0 0 0 0
Mitochondria 0 0 – – 0 0 0 – 0 0 0.09 0 – – 0 0 0 0 0 0 0 0 0 0
Nucleus 0 0 – – 0 0 0 – 0 0 0 0 – – 0 0 0 0 0 0 0 0 0 0
Nuclear Periphery 0 0 – – 0 0 0 – 0 0 0 0 – – 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 – – 0 0 0 – 0 0 0 0 – – 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 – – 0 0 0 – 0 0 0 0 – – 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 – – 0 0 0 – 0 0 0 0 – – 0 0 0 0 0 0 0 0 0 0
Vac/Vac Membrane 0 0 – – 0 0 0 – 0 0 0 0 – – 0 0 0 0 0 0 0 0 0 0
ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 – – 0 0 2 – 1 3 5 5 – – 0 0 0 0 0 0 0 0 0 0
Bud 1 0 – – 0 0 0 – 0 0 0 0 – – 0 0 0 1 1 0 0 0 0 0
Bud Neck 0 0 – – 0 0 0 – 0 0 0 0 – – 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 – – 0 0 0 – 0 0 0 0 – – 0 0 0 0 – – – – – –
Cell Periphery 1 2 – – 5 9 10 – 3 13 8 15 – – 4 33 25 26 0 0 0 0 0 0
Cytoplasm 120 76 – – 145 166 176 – 137 129 104 142 – – 54 300 254 226 112 91 22 17 59 30
Endoplasmic Reticulum 2 0 – – 2 2 5 – 6 10 12 9 – – 0 9 12 5 1 0 0 0 0 0
Endosome 0 0 – – 0 0 0 – 0 0 0 0 – – 0 0 0 0 0 0 0 0 0 0
Golgi 0 0 – – 0 0 0 – 0 1 0 0 – – 0 1 0 0 0 0 0 0 0 0
Mitochondria 3 0 – – 0 1 0 – 1 6 12 4 – – 0 3 0 0 2 0 0 0 0 0
Nucleus 1 0 – – 0 0 0 – 1 0 1 2 – – 0 3 2 0 0 0 0 0 1 1
Nuclear Periphery 0 0 – – 0 0 0 – 0 0 0 0 – – 0 0 0 0 0 0 0 0 1 0
Nucleolus 0 0 – – 0 0 0 – 0 0 0 0 – – 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 – – 0 0 0 – 0 0 0 0 – – 0 1 0 0 0 0 0 0 0 0
SpindlePole 0 0 – – 0 0 0 – 0 0 0 0 – – 0 0 0 0 0 0 0 0 0 0
Vac/Vac Membrane 0 0 – – 0 0 0 – 3 2 3 6 – – 0 1 2 2 0 1 0 0 1 0
Unique Cell Count 124 76 147 169 186 148 150 131 165 54 318 260 234 121 95 24 21 66 38
Labelled Cell Count 128 78 152 178 193 152 164 145 183 58 351 295 260 121 95 24 21 66 38


Cytoplasm

(Huh et al. Nature 2003 425:686)
Screen WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 AF100 AF140 AF180
Mean Cell GFP Intensity (1e-4) 19.7 29.2 – – 23.1 21.9 20.9 – 17.1 18.1 16.0 17.9 – – 28.0 36.2 35.3 34.1 – 22.2 –
Std Deviation (1e-4) 5.1 5.6 – – 3.9 3.7 4.4 – 3.9 4.3 3.7 4.5 – – 8.4 9.4 8.1 8.8 – 7.7 –
Intensity Change (Log2) – – – – – – – – – – – – – – – – – – – – –


Localization RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3
Actin – – – – – – – – – – – – – – –
Bud – – – – – – – – – – – – – – –
Bud Neck – – – – – – – – – – – – – – –
Bud Site – – – – – – – – – – – – – – –
Cell Periphery – – – – – – – – – – – – – – –
Cyto – – – – – – – – – – – – – – –
Endoplasmic Reticulum – – – – – – – – – – – – – – –
Endosome – – – – – – – – – – – – – – –
Golgi – – – – – – – – – – – – – – –
Mitochondria – – – – – – – – – – – – – – –
Nuclear Periphery – – – – – – – – – – – – – – –
Nuc – – – – – – – – – – – – – – –
Nucleolus – – – – – – – – – – – – – – –
Peroxisomes – – – – – – – – – – – – – – –
SpindlePole – – – – – – – – – – – – – – –
Vac – – – – – – – – – – – – – – –
Cortical Patches – – – – – – – – – – – – – – –
Cytoplasm – – – – – – – – – – – – – – –
Nucleus – – – – – – – – – – – – – – –
Vacuole – – – – – – – – – – – – – – –

Images

Circle plot not available

Protein Concentration and Protein Localization Data

R1 R2 R3
G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase
Concentration 15.9237 22.8316 20.2528 20.0038 13.6472 21.0389 16.6276 22.7725 19.4417 17.9633 15.0168 20.2903 19.0587 26.7243 22.7726 21.9648 16.916 23.5057
Actin 0.0082 0.0002 0.0069 0.0042 0.0011 0.0005 0.0149 0.0001 0.0004 0.0063 0.0274 0.0007 0.0278 0.0001 0.009 0.0381 0.0046 0.0001
Bud 0.0007 0.0009 0.0001 0.0017 0.0004 0.0002 0.001 0 0.0001 0.0003 0.0011 0.0003 0.0074 0 0.0021 0.0008 0.0011 0.0001
Bud Neck 0.0009 0.0002 0.0003 0.0006 0.0006 0.0008 0.0003 0.0001 0.0002 0.0003 0.0016 0.0005 0.011 0.0001 0.0014 0.0013 0.001 0.0004
Bud Periphery 0.0005 0.0002 0.0001 0.0004 0.0002 0.0003 0.0017 0 0 0.0004 0.001 0.0002 0.0024 0 0.0006 0.001 0.0007 0
Bud Site 0.0067 0.0009 0.0002 0.0004 0.0029 0 0.0038 0 0.0001 0.0011 0.0116 0.0001 0.0148 0 0.0008 0.0009 0.0022 0
Cell Periphery 0.0002 0.0003 0 0.0003 0.0004 0.0001 0.0002 0 0 0.0002 0.0004 0 0.0006 0 0.0002 0.0002 0.0003 0
Cytoplasm 0.8758 0.9883 0.9589 0.9795 0.7094 0.9613 0.8793 0.9966 0.956 0.9301 0.7302 0.9471 0.8377 0.9968 0.9231 0.8213 0.7548 0.9946
Cytoplasmic Foci 0.0139 0.002 0.0022 0.0044 0.0636 0.0023 0.018 0.0005 0.0088 0.0047 0.0297 0.014 0.0186 0.0005 0.0139 0.0051 0.0661 0.0011
Eisosomes 0.0003 0 0 0 0.0001 0 0.0001 0 0 0.0001 0.0002 0 0.0003 0 0.0001 0.0019 0.0003 0
Endoplasmic Reticulum 0.0051 0.0006 0.0064 0.0011 0.0114 0.0032 0.005 0.0016 0.0011 0.0024 0.0105 0.0027 0.0068 0.0002 0.0019 0.0016 0.0158 0.0009
Endosome 0.03 0.0009 0.0063 0.0004 0.0896 0.0096 0.0212 0.0001 0.0036 0.0258 0.052 0.011 0.0172 0.0003 0.0107 0.0275 0.0385 0.0008
Golgi 0.0097 0.0001 0.0043 0.0001 0.01 0.0006 0.0058 0 0.0015 0.0086 0.0281 0.0015 0.0043 0 0.0027 0.0129 0.0029 0.0001
Lipid Particles 0.0058 0 0.0038 0.0001 0.0154 0.0001 0.0075 0 0.0057 0.005 0.0184 0.0014 0.0089 0 0.007 0.0055 0.0245 0
Mitochondria 0.0186 0.0018 0.0036 0.0002 0.0032 0.0138 0.0065 0 0.0027 0.0074 0.0214 0.0062 0.0036 0 0.0042 0.0101 0.0047 0.0001
None 0.0026 0.0009 0.0002 0.0021 0.0453 0.0005 0.0013 0.0003 0.0096 0.0013 0.007 0.0021 0.0072 0.0001 0.0056 0.0428 0.0236 0.0002
Nuclear Periphery 0.004 0.0003 0.0009 0.0008 0.0093 0.0016 0.0028 0.0001 0.0005 0.0009 0.0024 0.0033 0.0061 0.0002 0.0014 0.0033 0.0118 0.0004
Nucleolus 0.0006 0.0004 0 0 0.0008 0 0.0003 0 0.0001 0.0001 0.0001 0.0004 0.0018 0 0.0006 0.0011 0.0021 0
Nucleus 0.0038 0.001 0.0013 0.0019 0.0038 0.0016 0.0016 0.0004 0.0011 0.0008 0.0014 0.0031 0.0055 0.0015 0.0015 0.003 0.011 0.0009
Peroxisomes 0.0023 0.0001 0.001 0.0001 0.0012 0 0.0129 0 0.0038 0.0011 0.0457 0.0009 0.0052 0 0.0039 0.0027 0.0033 0
Punctate Nuclear 0.0022 0.0001 0.0001 0.0006 0.0046 0.0001 0.0123 0 0.0039 0.0002 0.0011 0.001 0.0071 0 0.0054 0.0026 0.0138 0
Vacuole 0.0057 0.0007 0.0025 0.001 0.0206 0.0023 0.0023 0.0001 0.0006 0.002 0.0057 0.0024 0.0042 0.0002 0.0028 0.0118 0.0138 0.0002
Vacuole Periphery 0.0021 0.0002 0.0007 0.0002 0.0059 0.001 0.0013 0 0.0002 0.0011 0.0032 0.0011 0.0013 0 0.0012 0.0043 0.0032 0.0001

Sequencing Data

R1 R2
G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Post-START S/G2 Metaphase Anaphase Telophase
Gene Expression 65.5457 73.3117 85.3098 100.1079 78.1359 81.9644 107.6784 118.4414 124.1455 97.8369
Translational Efficiency 1.6845 1.6669 1.4612 1.3518 1.1526 1.4814 1.4084 1.3099 1.2122 1.1174

Hit Data

Dataset Hit
Protein Concentration ✔
Protein Localization ✔
Gene Expression ✔
Translational Efficiency ✘

Micrographs

Cell Count

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
1664 1270 868 1297 1457 824 1905 97 3121 2094 2773 1394

Protein Abundance

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
Mean 1372.88 1667.38 2932.77 2432.89 2029.06 1658.24 2550.95 2622.60 1679.21 1663.78 2670.47 2446.09
Standard Deviation 271.10 364.72 478.57 498.78 371.77 303.45 389.93 547.54 459.21 341.95 455.51 504.64
Intensity Change Log 2 — 0.280377 1.095058 0.825466 — -0.291159 0.330223 0.370186 — -0.032734 0.688797 0.571493

Localization Score (DeepLoc)

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
Actin 0.000153 0.000108 0.000158 0.000581 0.000112 0.000214 0.000104 0.000802 0.000134 0.000150 0.000121 0.000597
Bud Neck 0.000289 0.000246 0.001974 0.002081 0.000356 0.000372 0.000833 0.006944 0.000320 0.000296 0.001190 0.002419
Bud Site 0.015259 0.003994 0.002284 0.017129 0.003783 0.006596 0.003227 0.012365 0.009902 0.005018 0.002932 0.016798
Cell Periphery 0.000317 0.000230 0.002187 0.000767 0.000530 0.000216 0.000613 0.000977 0.000417 0.000225 0.001106 0.000782
Cytoplasm 0.900303* 0.954796* 0.859930* 0.849134* 0.942626* 0.946140* 0.905788* 0.696505* 0.920061* 0.951390* 0.891434* 0.838514*
Cytoplasmic Foci 0.038419 0.006701 0.011276 0.010979 0.011842 0.014210 0.005247 0.013096 0.026012 0.009656 0.007134 0.011127
Eisosomes 0.000002 0.000001 0.000002 0.000005 0.000001 0.000002 0.000001 0.000019 0.000002 0.000001 0.000001 0.000006
Endoplasmic Reticulum 0.002137 0.000240 0.000078 0.000206 0.000509 0.000517 0.000108 0.000347 0.001377 0.000349 0.000099 0.000216
Endosome 0.001343 0.000390 0.000557 0.005950 0.000840 0.001155 0.000627 0.021206 0.001108 0.000691 0.000605 0.007012
Golgi 0.006980 0.002574 0.007502 0.009443 0.009148 0.004449 0.002754 0.014028 0.007992 0.003312 0.004241 0.009762
Lipid Particles 0.000656 0.000950 0.004915 0.004469 0.002183 0.001334 0.003651 0.007787 0.001369 0.001101 0.004047 0.004700
Mitochondria 0.000842 0.000046 0.000073 0.002761 0.000039 0.000180 0.000045 0.006035 0.000467 0.000098 0.000054 0.002988
Mitotic Spindle 0.001318 0.000394 0.001407 0.006985 0.001177 0.000775 0.001522 0.010794 0.001252 0.000544 0.001486 0.007250
None 0.003279 0.003680 0.003373 0.001109 0.002023 0.003153 0.001251 0.000751 0.002693 0.003472 0.001915 0.001084
Nuclear Periphery 0.000090 0.000019 0.000336 0.000124 0.000036 0.000021 0.000166 0.000131 0.000064 0.000020 0.000219 0.000125
Nuclear Periphery Foci 0.000710 0.002077 0.005822 0.002102 0.003848 0.002683 0.002873 0.003111 0.002175 0.002316 0.003796 0.002172
Nucleolus 0.000102 0.000101 0.000068 0.000183 0.000028 0.000093 0.000104 0.000272 0.000067 0.000098 0.000093 0.000189
Nucleus 0.006990 0.014501 0.060744 0.018557 0.010636 0.008666 0.020406 0.014365 0.008692 0.012205 0.033033 0.018265
Peroxisomes 0.000186 0.000072 0.000012 0.000657 0.000023 0.000097 0.000037 0.007168 0.000110 0.000082 0.000029 0.001110
Vacuole 0.009562 0.007293 0.034819 0.063080 0.006796 0.006839 0.049012 0.177507 0.008271 0.007114 0.044569 0.071042
Vacuole Periphery 0.011062 0.001588 0.002485 0.003697 0.003465 0.002286 0.001630 0.005789 0.007515 0.001862 0.001897 0.003842

Localization Changes (T-score)

R1 R2 R1 & R2
UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14 UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14 UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14
Actin 1.88 -3.04 -3.41 -3.81 -2.63 -3.15 -2.67 -1.70 -1.11 -1.49 -1.38 -3.25 -3.82 -3.49 -3.32
Bud Neck 0.79 -9.71 -11.77 -11.86 -0.46 -0.28 -6.90 -4.83 -4.81 -3.95 0.20 -11.75 -12.42 -12.47 -4.97
Bud Site 9.62 12.06 -0.41 -7.15 -8.40 -2.21 -0.81 -3.23 -1.67 -3.05 5.67 8.80 -3.59 -6.71 -7.90
Cell Periphery 1.76 -11.62 -6.96 -8.18 8.88 3.21 -1.13 -2.64 -4.42 -2.26 3.61 -9.71 -5.31 -9.62 4.82
Cytoplasm -10.97 8.91 11.84 21.39 1.97 -0.34 13.61 10.84 10.84 8.68 -8.69 13.48 17.87 22.79 8.93
Cytoplasmic Foci 13.80 10.02 11.20 -4.23 2.00 -1.82 5.35 -1.98 -0.97 -4.18 11.77 12.61 9.26 -1.78 -2.61
Eisosomes 5.80 -0.66 -4.09 -6.87 -3.70 -1.92 -7.56 -3.12 -2.64 -2.86 0.48 -2.03 -6.34 -5.91 -5.82
Endoplasmic Reticulum 4.03 4.37 4.26 1.71 -1.86 -0.48 4.85 3.03 1.80 -0.89 3.63 5.02 4.85 2.43 -1.40
Endosome 5.36 3.52 -3.20 -4.55 -4.14 -1.01 0.50 -1.94 -1.86 -1.96 2.67 3.02 -4.00 -4.54 -4.50
Golgi 7.77 -1.25 0.96 -2.74 1.68 3.92 6.92 0.91 -0.29 -0.74 7.70 6.26 1.88 -2.15 -1.26
Lipid Particles -1.74 -9.07 -10.46 -9.65 0.66 2.06 -5.63 -2.70 -2.91 -2.06 1.21 -11.31 -9.10 -9.53 -1.84
Mitochondria 5.06 4.82 -1.24 -2.59 -2.52 -1.48 -0.99 -1.12 -1.04 -1.11 4.00 4.86 -2.03 -2.68 -2.75
Mitotic Spindle 2.92 -0.51 -3.17 -4.13 -2.97 1.55 -0.59 -0.96 -1.07 -0.86 3.48 -0.63 -3.43 -4.15 -3.04
None -0.38 0.10 2.54 4.86 6.98 -0.94 3.76 6.41 2.29 4.72 -1.04 1.80 3.47 4.38 5.05
Nuclear Periphery 1.75 -6.11 -1.04 -8.01 7.00 1.84 -1.77 -6.66 -6.98 -0.77 2.03 -3.29 -3.01 -8.81 2.09
Nuclear Periphery Foci -5.37 -9.22 -8.24 -2.18 6.02 1.62 0.84 -1.24 -2.10 -1.67 -0.78 -6.07 -2.78 -1.77 4.00
Nucleolus -0.15 1.06 -3.30 -1.51 -5.71 -1.07 -4.03 -4.03 -1.13 -1.37 -0.91 -2.32 -5.83 -1.90 -3.20
Nucleus -5.67 -19.69 -11.89 -6.93 12.53 1.29 -11.77 -4.31 -4.76 0.42 -4.18 -22.42 -12.11 -9.28 7.00
Peroxisomes 3.88 7.28 -1.11 -1.60 -1.86 -2.66 -2.73 -1.10 -1.07 -1.09 1.22 4.36 -1.80 -1.88 -2.04
Vacuole 1.52 -9.76 -21.83 -22.35 -9.44 -0.63 -19.77 -10.62 -10.58 -8.28 0.66 -21.63 -23.75 -23.81 -10.51
Vacuole Periphery 6.86 6.13 6.32 -1.10 0.14 1.08 2.66 0.69 -0.05 -0.57 6.36 7.17 5.73 -0.78 -1.08
Standard name
Human Ortholog
Description Enzyme of 'de novo' purine biosynthesis; contains both 5-aminoimidazole-4-carboxamide ribonucleotide transformylase and inosine monophosphate cyclohydrolase activities; ADE16 has a paralog, ADE17, that arose from the whole genome duplication; ade16 ade17 mutants require adenine and histidine
Localization
Cell Percentages cytoplasm (99%)
Cell Cycle Regulation No
Subcompartmental Group cytoplasm-7

Ade16

Ade16


Nearest neighbours ( genes)

correlation threshold

0.6

1.0

0.80


Biological Process (Gene Ontology)
Molecular Function (Gene Ontology)
Cellular Component (Gene Ontology)

Endocytosis
Temp Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Vph1-GFP)
37℃
RT
Cell Cycle Omics

CYCLoPs (Ade16-GFP)
Gene / Allele Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Sac6-tdTomato)
Gene Images
Gene Images
Images are not yet available
Images are not yet available