Standard name
Human Ortholog
Description DNA helicase/Ubiquitin ligase; involved in error-free DNA damage tolerance (DDT), replication fork regression during postreplication repair by template switching, error-prone translesion synthesis; promotes synthesis of free and PCNA-bound polyubiquitin chains by Ubc13p-Mms2p; forms nuclear foci upon DNA replication stress; associates with native telomeres, cooperates with homologous recombination in senescent cells; human homolog HLTF can complement yeast null mutant
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Micrographs

ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0.06 0 0
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 – – – – – –
Cell Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cytoplasm 0.55 0.4 0.61 0.43 0.46 0.68 0.76 0.74 0.73 0.69 0.66 0.7 0.41 0.5 0.59 0.34 0.36 0.25 0.27 0.25 0.36 0.2 0.37 0.49
Endoplasmic Reticulum 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0.07 0 0 0 0 0 0
Endosome 0.05 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0.06 0 0 0 0 0 0 0
Golgi 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Mitochondria 0.06 0.61 0.38 0.48 0.37 0.42 0.34 0.4 0.46 0.51 0.54 0.53 0.1 0.08 0.32 0.05 0 0.06 0.08 0 0.09 0 0 0
Nucleus 0.24 0.11 0.08 0.29 0.21 0.07 0.05 0 0 0 0 0.05 0.39 0.32 0.09 0.46 0.4 0.45 0.32 0.33 0.19 0.33 0.22 0.11
Nuclear Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0.05 0.08 0.05 0 0 0
Vac/Vac Membrane 0.18 0.32 0 0 0.11 0 0 0 0 0 0 0 0.16 0.14 0.15 0.25 0.26 0.28 0.22 0.2 0.21 0.24 0.24 0.22
ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 0 0 0 0 0 0 0 0 1 0 0 0 0 1 0 0 0 0
Bud 1 0 2 1 1 4 4 9 3 7 8 2 0 1 6 0 0 0 7 4 1 7 6 7
Bud Neck 0 0 1 0 1 0 3 1 1 0 1 2 0 1 0 0 0 0 0 1 0 1 1 3
Bud Site 0 0 1 1 0 2 4 3 0 1 3 5 0 0 0 0 0 0 – – – – – –
Cell Periphery 0 0 0 0 0 0 0 1 0 1 1 1 0 0 0 0 0 0 0 0 0 0 0 0
Cytoplasm 192 37 94 70 123 222 323 354 151 269 206 250 73 84 118 58 43 59 131 27 59 26 74 147
Endoplasmic Reticulum 0 0 0 0 0 0 0 0 0 0 0 0 1 3 1 7 5 17 3 0 2 1 0 1
Endosome 17 0 0 0 3 0 0 0 0 0 0 0 4 5 1 4 7 8 3 1 1 2 0 2
Golgi 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 1 0 0 3 0 1
Mitochondria 21 56 59 77 100 136 145 189 96 198 170 187 18 13 64 8 5 15 37 4 15 1 6 12
Nucleus 85 10 12 47 57 24 23 17 4 16 13 16 70 54 18 80 48 106 151 35 31 45 44 33
Nuclear Periphery 1 0 0 0 0 0 1 1 0 1 2 0 1 1 1 0 0 0 2 0 0 0 0 0
Nucleolus 2 0 1 0 0 0 1 2 3 2 1 0 4 1 2 0 0 0 1 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 1 0 0 0 1 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 1 0 0 0 1 2 1 3 2 7 2 7 0 1 0 1 0 1 23 8 7 4 8 10
Vac/Vac Membrane 63 29 3 5 29 7 3 4 1 3 4 1 29 23 29 43 31 65 105 22 34 32 48 65
Unique Cell Count 351 92 155 161 268 325 426 477 208 391 312 355 178 169 199 173 120 235 480 109 165 135 201 301
Labelled Cell Count 383 132 173 201 315 397 508 585 261 505 411 472 200 187 241 201 139 271 480 109 165 135 201 301


Cytoplasm, Nucleus

(Huh et al. Nature 2003 425:686)
Screen WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 AF100 AF140 AF180
Mean Cell GFP Intensity (1e-4) 4.6 3.3 3.0 3.1 3.6 3.0 3.1 3.0 2.9 2.7 2.7 2.3 4.5 4.6 3.9 5.5 5.5 6.1 4.8 3.9 4.5
Std Deviation (1e-4) 0.8 0.6 0.7 0.9 1.6 1.3 1.8 1.5 1.9 1.9 1.7 1.4 1.1 1.5 1.4 1.5 1.4 1.4 2.4 1.1 2.0
Intensity Change (Log2) – – – 0.04 0.24 -0.03 0.03 -0.01 -0.09 -0.17 -0.16 -0.37 0.57 0.59 0.37 0.87 0.86 1.0 0.66 0.37 0.58


Localization RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3
Actin – – – – – – – – – – – – – – –
Bud – – – – – – – – – – – – 0 0 0
Bud Neck – – – – – – – – – – – – 0 0 0
Bud Site – – – – – – – – – – – – 0 0 0
Cell Periphery – – – – – – – – – – – – 0 0 0
Cyto – – – – – – – – – – – – – – –
Endoplasmic Reticulum – – – – – – – – – – – – 0 0 0
Endosome – – – – – – – – – – – – 0 0 0
Golgi – – – – – – – – – – – – 0 0 0
Mitochondria – – – – – – – – – – – – 0 0 0
Nuclear Periphery – – – – – – – – – – – – 0 0 0
Nuc – – – – – – – – – – – – – – –
Nucleolus – – – – – – – – – – – – 0 0 0
Peroxisomes – – – – – – – – – – – – 0 0 0
SpindlePole – – – – – – – – – – – – 0 0 0
Vac – – – – – – – – – – – – – – –
Cortical Patches – – – – – – – – – – – – 0 0 0
Cytoplasm – – – – – – – – – – – – 0 0 0
Nucleus – – – – – – – – – – – – 0 0 0
Vacuole – – – – – – – – – – – – 6.0 6.0 6.6

Images

Circle plot not available

Protein Concentration and Protein Localization Data

R1 R2 R3
G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase
Concentration 0.5726 1.5643 1.6393 0.9523 0.8789 1.3378 0.7488 1.2859 1.2978 1.5267 0.8404 0.761 -0.8328 -0.0098 -0.3329 0.8637 -0.6077 -0.5416
Actin 0.0002 0.0002 0.0001 0.0003 0.0002 0.0003 0.0507 0.0026 0.0001 0.0326 0.0004 0.0009 0.0245 0.0005 0.0001 0.0002 0.0002 0.0006
Bud 0.0008 0.0001 0 0.0001 0.0001 0.0001 0.0011 0.0002 0 0.0008 0.0001 0.0002 0.0004 0.0001 0 0 0.0002 0
Bud Neck 0.0012 0.0012 0.0003 0.0008 0.0016 0.0062 0.0015 0.0005 0.0004 0.0004 0.0006 0.0021 0.0012 0.0002 0.0002 0.0002 0.0012 0.0022
Bud Periphery 0.0008 0.0001 0 0.0001 0.0001 0.0001 0.0015 0.0002 0 0.0009 0 0.0002 0.0005 0 0 0 0.0003 0.0001
Bud Site 0.0017 0.004 0.0003 0.0001 0.0003 0.0003 0.0028 0.0053 0.0002 0.0024 0.0003 0.0002 0.0012 0.0005 0.0001 0 0.0001 0.0001
Cell Periphery 0.0002 0.0002 0 0 0 0.0001 0.0003 0.0001 0 0.0001 0 0 0.0001 0 0 0 0.0001 0
Cytoplasm 0.1102 0.0852 0.0345 0.0591 0.0519 0.1143 0.0743 0.0805 0.0188 0.0605 0.0587 0.0942 0.064 0.0628 0.0425 0.0282 0.0615 0.0497
Cytoplasmic Foci 0.004 0.0008 0.0008 0.0005 0.0013 0.0023 0.0264 0.0012 0.0025 0.0169 0.0342 0.0038 0.0075 0.0008 0.0004 0.0008 0.0009 0.0048
Eisosomes 0 0 0 0 0.0001 0 0.0003 0.0001 0 0.0001 0 0 0.0002 0 0 0.0001 0 0
Endoplasmic Reticulum 0.0087 0.0039 0.0015 0.003 0.0019 0.0023 0.0057 0.0035 0.0005 0.0013 0.004 0.0017 0.0039 0.0011 0.0014 0.0004 0.0012 0.0007
Endosome 0.006 0.0017 0.0013 0.0007 0.0021 0.0017 0.0293 0.0011 0.0008 0.0424 0.0471 0.0058 0.0059 0.0013 0.0005 0.0006 0.0013 0.0024
Golgi 0.0001 0.0001 0 0 0 0.0001 0.0157 0.0004 0.0008 0.0377 0.0027 0.0017 0.0019 0.0001 0 0 0 0.0003
Lipid Particles 0.0007 0.0003 0.0002 0.0001 0.0002 0.0001 0.0174 0.0006 0.0033 0.0198 0.0042 0.0252 0.0038 0.0001 0.0001 0.0001 0.0001 0.0016
Mitochondria 0.0015 0.0003 0.0004 0.0005 0.0004 0.0005 0.0071 0.0008 0.0004 0.0353 0.0009 0.016 0.0015 0.0003 0.0003 0.0002 0.0007 0.0004
None 0.1215 0.0559 0.027 0.08 0.1483 0.1268 0.0992 0.0901 0.007 0.0653 0.0979 0.0188 0.2754 0.1557 0.1159 0.4356 0.2365 0.1522
Nuclear Periphery 0.0251 0.0085 0.0099 0.0119 0.0135 0.0071 0.0328 0.0137 0.0091 0.0055 0.0145 0.0103 0.022 0.0264 0.0114 0.0109 0.0084 0.0075
Nucleolus 0.0115 0.0083 0.0058 0.0027 0.0086 0.0073 0.0128 0.003 0.0042 0.0019 0.0035 0.0053 0.01 0.0035 0.0053 0.0041 0.0053 0.0084
Nucleus 0.6741 0.8067 0.908 0.8286 0.705 0.7044 0.5704 0.7811 0.9393 0.6639 0.6974 0.7967 0.5559 0.7182 0.8039 0.4577 0.6607 0.7351
Peroxisomes 0.0001 0 0 0 0.0001 0.0001 0.0099 0.0013 0.0065 0.0056 0.0013 0.0021 0.0066 0.0001 0 0 0 0.0013
Punctate Nuclear 0.0216 0.02 0.0087 0.0103 0.0626 0.0239 0.0293 0.012 0.0051 0.0019 0.0289 0.0127 0.0097 0.0272 0.017 0.0606 0.0186 0.0318
Vacuole 0.0086 0.0024 0.0008 0.0009 0.0014 0.0018 0.0083 0.0014 0.0007 0.0033 0.0024 0.0016 0.0031 0.0008 0.0006 0.0002 0.0023 0.0006
Vacuole Periphery 0.0012 0.0002 0.0002 0.0004 0.0002 0.0002 0.0033 0.0003 0.0001 0.0014 0.0007 0.0006 0.0008 0.0002 0.0002 0.0001 0.0004 0.0001

Sequencing Data

R1 R2
G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Post-START S/G2 Metaphase Anaphase Telophase
Gene Expression 19.3332 5.3394 4.5876 12.2008 14.6729 12.2533 7.1034 5.082 9.9604 19.4037
Translational Efficiency 1.0536 0.7232 0.9818 0.7357 1.0685 1.6962 0.5471 0.6463 0.5897 0.7276

Hit Data

Dataset Hit
Protein Concentration ✔
Protein Localization ✘
Gene Expression ✔
Translational Efficiency ✘
Standard name
Human Ortholog
Description DNA helicase/Ubiquitin ligase; involved in error-free DNA damage tolerance (DDT), replication fork regression during postreplication repair by template switching, error-prone translesion synthesis; promotes synthesis of free and PCNA-bound polyubiquitin chains by Ubc13p-Mms2p; forms nuclear foci upon DNA replication stress; associates with native telomeres, cooperates with homologous recombination in senescent cells; human homolog HLTF can complement yeast null mutant
Localization
Cell Percentages nucleus (25%), cytoplasm (8%), mixed (47%)
Cell Cycle Regulation Yes
cytoplasm - MA (6.9e-11)
nucleus - T/G1 (1.7e-05)
Subcompartmental Group N/A

Rad5

Rad5


Nearest neighbours ( genes)

correlation threshold

0.6

1.0

0.80


Biological Process (Gene Ontology)
Molecular Function (Gene Ontology)
Cellular Component (Gene Ontology)

Endocytosis
Temp Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Vph1-GFP)
37℃
RT
Cell Cycle Omics

CYCLoPs (Rad5-GFP)
Gene / Allele Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Sac6-tdTomato)
Gene Images
Gene Images
Images are not yet available
Images are not yet available