Standard name
Human Ortholog
Description Protein involved in retrograde transport to the cis-Golgi network; forms heterodimer with Rgp1p that acts as a GTP exchange factor for Ypt6p; involved in transcription of rRNA and ribosomal protein genes
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Micrographs

ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0.05 0 0.09 0 0 0 0 0 0
Bud 0 0 0 0.05 0 0 0.06 0 0.07 0.05 0.06 0.08 0 0 0.05 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 – – – – – –
Cell Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cytoplasm 0 0 0 0.06 0 0.06 0.05 0 0 0 0 0 0.08 0.07 0.08 0.08 0.08 0.06 0 0 0 0 0 0
Endoplasmic Reticulum 0 0 0.06 0 0 0 0 0 0 0 0 0 0.05 0.09 0 0.06 0 0 0 0 0 0 0 0
Endosome 0.2 0 0 0 0 0 0 0 0 0 0 0 0.09 0.09 0 0.25 0.23 0.2 0.14 0.23 0.24 0.24 0.2 0.16
Golgi 0.31 0 0 0 0 0 0 0 0 0 0 0 0.18 0.18 0 0.33 0.43 0.47 0.66 0.26 0.24 0.4 0.42 0.54
Mitochondria 0.67 0.94 0.95 0.94 0.92 0.95 0.95 0.96 0.99 0.96 0.96 0.98 0.78 0.68 0.89 0.16 0.17 0.14 0.1 0.4 0.32 0.21 0.25 0.16
Nucleus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nuclear Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0.05 0 0 0 0 0 0 0 0 0 0 0 0 0 0.05 0 0.14 0.05 0 0.06
SpindlePole 0 0 0 0 0.06 0.05 0 0 0 0 0 0 0 0 0 0.13 0.07 0.08 0 0 0 0 0 0
Vac/Vac Membrane 0.06 0.09 0 0 0 0 0 0 0 0 0 0 0.07 0.07 0 0.11 0.14 0.11 0 0 0 0.05 0 0
ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 7 2 2 2 0 0 1 1 0 0 0 0 2 8 3 25 10 51 2 4 4 3 7 16
Bud 4 2 7 11 11 13 26 17 26 22 15 30 5 9 24 16 17 22 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 1 1 3 6 12 12 6 5 7 4 0 0 0 0 0 0 – – – – – –
Cell Periphery 0 1 0 0 0 1 0 2 0 1 3 5 0 0 0 5 7 8 0 0 0 0 0 0
Cytoplasm 2 4 5 13 7 17 23 14 8 17 8 5 19 25 41 45 31 31 0 0 0 0 1 2
Endoplasmic Reticulum 2 6 12 2 3 4 5 7 0 4 5 2 13 32 20 30 17 17 0 0 1 1 3 4
Endosome 38 0 0 0 9 1 4 2 0 0 0 1 21 32 9 134 92 107 28 34 52 43 81 72
Golgi 58 2 2 0 0 0 1 0 0 0 0 0 43 65 23 176 176 256 140 39 52 73 175 237
Mitochondria 127 129 186 199 246 278 437 459 386 423 247 352 187 246 459 85 70 74 21 60 70 38 104 72
Nucleus 2 0 0 0 0 1 3 0 1 2 1 2 0 1 0 6 2 4 0 0 0 0 1 0
Nuclear Periphery 0 1 0 0 1 0 0 2 3 1 2 4 0 1 1 2 2 2 1 0 0 1 1 1
Nucleolus 0 0 0 1 0 2 3 1 2 3 2 3 0 0 3 4 1 6 0 0 0 0 0 0
Peroxisomes 6 0 3 5 13 3 9 5 1 4 0 3 3 0 2 6 3 3 11 2 30 9 13 27
SpindlePole 6 1 6 2 17 14 16 16 6 12 5 4 0 4 1 71 30 44 0 0 1 0 3 1
Vac/Vac Membrane 12 13 4 1 6 4 10 9 5 2 0 7 16 27 18 60 56 59 4 4 2 9 16 2
Unique Cell Count 189 137 195 211 268 293 462 479 391 441 258 360 239 362 515 530 408 542 212 151 218 184 416 444
Labelled Cell Count 264 161 228 237 316 344 550 547 444 496 295 422 309 450 604 665 514 684 212 151 218 184 416 444


Golgi

(Huh et al. Nature 2003 425:686)
Screen WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 AF100 AF140 AF180
Mean Cell GFP Intensity (1e-4) 6.7 4.6 4.1 4.0 4.1 3.6 3.6 3.6 3.2 3.2 3.3 3.0 6.0 6.2 5.3 7.6 8.3 8.2 5.8 5.7 6.2
Std Deviation (1e-4) 0.7 0.6 0.6 1.1 0.9 0.8 0.9 0.9 0.8 0.9 0.9 0.5 0.8 0.7 0.8 1.0 1.4 1.2 0.7 1.0 1.3
Intensity Change (Log2) – – – -0.01 0.03 -0.16 -0.19 -0.18 -0.34 -0.32 -0.29 -0.44 0.56 0.6 0.39 0.9 1.03 1.02 0.53 0.49 0.61


Localization RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3
Actin – – – – – – – – – – – – – – –
Bud – – – – – – – – – – – – 0 0 0
Bud Neck – – – – – – – – – – – – 0 0 0
Bud Site – – – – – – – – – – – – 0 0 0
Cell Periphery – – – – – – – – – – – – 0 0 0
Cyto – – – – – – – – – – – – – – –
Endoplasmic Reticulum – – – – – – – – – – – – 0 0 0
Endosome – – – – – – – – – – – – 0 0 0
Golgi – – – – – – – – – – – – 0 0 0
Mitochondria – – – – – – – – – – – – 0 0 0
Nuclear Periphery – – – – – – – – – – – – 0 0 0
Nuc – – – – – – – – – – – – – – –
Nucleolus – – – – – – – – – – – – 0 0 0
Peroxisomes – – – – – – – – – – – – 0 0 0
SpindlePole – – – – – – – – – – – – 0 0 0
Vac – – – – – – – – – – – – – – –
Cortical Patches – – – – – – – – – – – – 0 0 0
Cytoplasm – – – – – – – – – – – – 0 0 0
Nucleus – – – – – – – – – – – – 0 0 0
Vacuole – – – – – – – – – – – – 3.9 4.5 3.8

Images

Circle plot not available

Protein Concentration and Protein Localization Data

R1 R2 R3
G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase
Concentration 1.3553 1.9187 1.7497 1.5878 1.1858 1.8686 2.1586 2.83 2.4508 2.0647 2.0473 2.5763 1.8944 2.4696 2.1141 2.0599 1.8345 2.1443
Actin 0.0908 0.0099 0.0198 0.0031 0.0031 0.0206 0.0618 0.0037 0.0197 0.0012 0.0005 0.0195 0.0578 0.0172 0.0307 0.0049 0.0005 0.0226
Bud 0.0006 0.0005 0.0004 0.0245 0.0001 0.0002 0.0003 0.0001 0.0004 0.0001 0 0.0004 0.0004 0.0003 0.0003 0.0002 0.0002 0.0002
Bud Neck 0.0034 0.0008 0.0006 0.0007 0.0002 0.0011 0.0004 0.0003 0.0009 0.0002 0.0001 0.0004 0.0009 0.0006 0.0014 0.0004 0.0001 0.0008
Bud Periphery 0.0007 0.0003 0.0006 0.0151 0.0002 0.0003 0.0003 0.0001 0.0003 0 0 0.0009 0.0004 0.0004 0.0003 0.0002 0.0001 0.0002
Bud Site 0.0069 0.0044 0.0022 0.0029 0.0002 0.0004 0.0008 0.0006 0.0063 0.0003 0 0.0003 0.0012 0.0059 0.0015 0.0006 0.0001 0.0003
Cell Periphery 0.0005 0.0001 0.0001 0.0005 0.0001 0.0001 0.0001 0.0001 0.0001 0 0 0.0001 0.0002 0.0003 0.0001 0.0001 0 0.0002
Cytoplasm 0.0043 0.0057 0.0024 0.0066 0.0012 0.0023 0.0241 0.0077 0.0077 0.0024 0.001 0.0192 0.0091 0.012 0.0134 0.0156 0.02 0.0058
Cytoplasmic Foci 0.1181 0.1512 0.1291 0.0906 0.0844 0.0943 0.1436 0.1505 0.1448 0.1555 0.1192 0.0744 0.1413 0.1743 0.1375 0.1464 0.1335 0.1373
Eisosomes 0.0011 0.0002 0.0002 0.0001 0 0.0001 0.0002 0.0001 0.0001 0 0 0.0001 0.0005 0.0003 0.0002 0.0001 0 0.0002
Endoplasmic Reticulum 0.004 0.0011 0.0008 0.0013 0.0009 0.0013 0.0031 0.0015 0.0009 0.0008 0.0004 0.0035 0.0047 0.0025 0.0007 0.0019 0.0006 0.001
Endosome 0.214 0.2563 0.2179 0.2373 0.2305 0.2046 0.3235 0.3599 0.2895 0.5147 0.3296 0.2569 0.2238 0.2601 0.214 0.2829 0.3086 0.187
Golgi 0.3333 0.4273 0.4503 0.492 0.5108 0.4668 0.3291 0.4064 0.428 0.2237 0.4338 0.5022 0.3563 0.3619 0.4313 0.3348 0.3758 0.4055
Lipid Particles 0.0798 0.0388 0.0383 0.0141 0.0104 0.0642 0.0445 0.0215 0.0153 0.0235 0.1018 0.0331 0.0527 0.0119 0.0107 0.0764 0.0437 0.0788
Mitochondria 0.0317 0.026 0.0586 0.0908 0.1143 0.0747 0.0103 0.0266 0.0371 0.0054 0.0036 0.0582 0.0702 0.0871 0.0718 0.0229 0.044 0.0475
None 0.0079 0.0135 0.0006 0.0011 0.0001 0.0008 0.009 0.0016 0.0009 0.0002 0 0.0006 0.01 0.0106 0.0158 0.0148 0.0006 0.0132
Nuclear Periphery 0.0164 0.0003 0.0007 0.0002 0.0003 0.0015 0.0017 0.0003 0.0004 0.0003 0.0001 0.0029 0.0038 0.0004 0.0002 0.0007 0.0001 0.0004
Nucleolus 0.0004 0.0001 0.0004 0.0002 0.0001 0.0001 0.0002 0.0001 0.0001 0 0 0 0.0002 0.0001 0.0001 0.0002 0 0.0011
Nucleus 0.002 0.0001 0.0006 0.0002 0.0001 0.0005 0.0004 0.0001 0.0002 0.0001 0 0.0004 0.0008 0.0001 0.0001 0.0002 0 0.0009
Peroxisomes 0.074 0.0588 0.0729 0.0134 0.0373 0.0603 0.0343 0.0142 0.0315 0.0639 0.0067 0.0218 0.0608 0.0504 0.0661 0.0898 0.0677 0.0912
Punctate Nuclear 0.0042 0.0005 0.0008 0.0003 0.0001 0.0009 0.0073 0.0002 0.0022 0.0007 0.0001 0.0003 0.0018 0.0004 0.0003 0.0008 0.0002 0.0018
Vacuole 0.003 0.0022 0.0013 0.0023 0.0021 0.0021 0.0028 0.0027 0.0107 0.0032 0.001 0.0021 0.0015 0.0019 0.0017 0.0037 0.0013 0.0023
Vacuole Periphery 0.0029 0.0018 0.0016 0.0027 0.0034 0.0027 0.0023 0.0019 0.0029 0.0039 0.0017 0.0028 0.0017 0.0014 0.0016 0.0025 0.0026 0.0017

Sequencing Data

R1 R2
G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Post-START S/G2 Metaphase Anaphase Telophase
Gene Expression 10.613 10.5761 12.2335 14.693 10.3766 6.9555 14.7822 14.1163 13.5184 14.8737
Translational Efficiency 0.5965 0.6301 0.532 0.4355 0.6576 0.9514 0.492 0.4017 0.5002 0.5246

Hit Data

Dataset Hit
Protein Concentration ✘
Protein Localization ✘
Gene Expression ✔
Translational Efficiency ✘

Micrographs

Cell Count

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
1833 1363 1895 640 1796 1839 145 365 3629 3202 2040 1005

Protein Abundance

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
Mean 743.45 747.07 1155.76 930.53 762.51 809.42 1125.88 946.32 752.88 782.88 1153.64 936.26
Standard Deviation 86.13 109.43 142.28 123.04 100.37 106.59 105.10 108.38 93.93 112.13 140.17 118.17
Intensity Change Log 2 — 0.007008 0.636534 0.323817 — 0.086132 0.562225 0.311572 — 0.047613 0.599388 0.317630

Localization Score (DeepLoc)

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
Actin 0.000899 0.002748 0.003348 0.001588 0.001475 0.000481 0.015656 0.000498 0.001184 0.001446 0.004223 0.001192
Bud Neck 0.000954 0.000322 0.000219 0.001470 0.000078 0.000233 0.001320 0.001165 0.000520 0.000271 0.000298 0.001359
Bud Site 0.001824 0.001018 0.004362 0.005890 0.000527 0.000499 0.013958 0.003850 0.001182 0.000720 0.005044 0.005149
Cell Periphery 0.000058 0.000052 0.000045 0.000045 0.000039 0.000033 0.000102 0.000045 0.000049 0.000041 0.000049 0.000045
Cytoplasm 0.004390 0.001348 0.001967 0.002126 0.000855 0.001080 0.002494 0.001802 0.002641 0.001194 0.002005 0.002008
Cytoplasmic Foci 0.209810* 0.184925* 0.263139* 0.271291* 0.175650 0.156707 0.370754* 0.241061* 0.192904* 0.168719 0.270788* 0.260312*
Eisosomes 0.000123 0.000086 0.000023 0.000023 0.000058 0.000054 0.000068 0.000041 0.000091 0.000068 0.000026 0.000030
Endoplasmic Reticulum 0.000415 0.000292 0.002146 0.000313 0.000204 0.000303 0.003170 0.000460 0.000311 0.000298 0.002218 0.000366
Endosome 0.062900 0.176086 0.099635 0.391845* 0.122539 0.202944* 0.236915* 0.439147* 0.092415 0.191511 0.109393 0.409025*
Golgi 0.614376* 0.561319* 0.571179* 0.209329* 0.660878* 0.532353* 0.230017* 0.167933* 0.637390* 0.544683* 0.546930* 0.194295*
Lipid Particles 0.013648 0.021285 0.003650 0.014010 0.008511 0.034598 0.020015 0.014571 0.011106 0.028931 0.004813 0.014214
Mitochondria 0.065734 0.031259 0.001430 0.006212 0.019031 0.042724 0.002317 0.008102 0.042621 0.037843 0.001493 0.006899
Mitotic Spindle 0.005329 0.002583 0.021690 0.038054 0.001330 0.004280 0.037872 0.054319 0.003350 0.003558 0.022840 0.043961
None 0.000121 0.000076 0.000021 0.000062 0.000027 0.000184 0.000014 0.000023 0.000074 0.000138 0.000020 0.000048
Nuclear Periphery 0.000027 0.000039 0.000281 0.000163 0.000014 0.000100 0.000488 0.000348 0.000020 0.000074 0.000296 0.000230
Nuclear Periphery Foci 0.000234 0.000471 0.002940 0.001272 0.000119 0.000452 0.009963 0.003419 0.000177 0.000460 0.003439 0.002052
Nucleolus 0.000209 0.000257 0.000005 0.000257 0.000011 0.000204 0.000107 0.000230 0.000111 0.000226 0.000012 0.000247
Nucleus 0.000307 0.000173 0.000030 0.000523 0.000013 0.000273 0.000349 0.000274 0.000161 0.000230 0.000052 0.000433
Peroxisomes 0.013076 0.009802 0.000685 0.014627 0.004728 0.008900 0.014845 0.011956 0.008944 0.009284 0.001691 0.013657
Vacuole 0.002101 0.002252 0.016098 0.032065 0.001342 0.007004 0.031715 0.040238 0.001725 0.004981 0.017208 0.035033
Vacuole Periphery 0.003467 0.003609 0.007107 0.008832 0.002569 0.006595 0.007862 0.010519 0.003023 0.005324 0.007161 0.009445

Localization Changes (T-score)

R1 R2 R1 & R2
UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14 UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14 UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14
Actin -2.92 -3.47 -1.28 1.04 1.62 3.94 -2.39 3.37 -0.09 2.56 -0.86 -3.86 -0.28 0.25 3.27
Bud Neck 2.00 2.38 -1.26 -3.72 -4.16 -4.55 -4.55 -5.37 -4.57 0.41 1.56 1.38 -3.34 -5.26 -5.15
Bud Site 1.93 -3.45 -2.52 -3.04 -0.90 0.27 -3.04 -4.82 -4.87 2.24 2.19 -5.42 -3.75 -4.22 -0.12
Cell Periphery 0.74 1.65 0.84 0.46 -0.01 0.45 -3.22 -0.35 -0.98 3.02 0.99 -0.10 0.28 -0.43 0.41
Cytoplasm 5.30 4.15 2.20 -1.71 -0.80 -0.74 -2.84 -1.83 -1.50 0.66 4.52 1.48 0.49 -2.37 -0.59
Cytoplasmic Foci 5.31 -10.64 -7.32 -10.25 -0.93 4.83 -12.44 -6.66 -8.61 7.20 7.93 -18.04 -10.55 -14.38 1.53
Eisosomes 2.46 9.30 9.02 5.77 -0.42 0.78 -0.90 0.74 0.57 1.08 3.06 10.86 5.90 3.84 -0.48
Endoplasmic Reticulum 1.32 -5.37 1.06 -0.38 5.80 -0.89 -4.40 -2.67 -1.12 3.99 0.15 -6.42 -0.95 -0.90 6.22
Endosome -24.92* -12.95 -31.56* -19.52 -28.02* -14.68 -8.74 -24.05 -17.28 -11.12 -26.99 -5.91 -38.39* -25.08 -35.92*
Golgi 6.35 5.55 41.64* 35.44* 37.80* 17.03 27.91 49.10* 33.74* 3.63 16.32 13.78 62.88* 47.32* 43.31*
Lipid Particles -2.62 7.49 -0.08 1.79 -3.28 -8.39 -1.99 -1.61 4.36 0.83 -8.19 6.13 -1.21 4.75 -3.83
Mitochondria 7.97 19.10 16.22 8.09 -3.11 -7.68 9.33 4.29 10.42 -2.62 1.77 21.40 15.88 13.72 -4.36
Mitotic Spindle 2.57 -7.58 -6.50 -7.01 -3.18 -3.04 -4.46 -6.41 -6.03 -1.49 -0.29 -9.69 -9.24 -9.15 -4.54
None 1.72 6.11 1.23 0.24 -0.99 -1.55 1.91 0.50 1.59 -1.15 -1.07 6.06 0.87 1.37 -1.03
Nuclear Periphery -0.90 -2.98 -6.52 -5.12 1.35 -3.71 -3.37 -6.01 -4.14 0.92 -3.69 -3.45 -8.62 -5.58 0.79
Nuclear Periphery Foci -0.65 -4.98 -3.53 -2.72 3.06 -3.40 -2.12 -3.59 -3.22 1.36 -1.77 -5.63 -4.96 -4.27 1.99
Nucleolus -0.22 2.09 -0.39 -0.09 -2.89 -2.14 -1.71 -1.90 -0.19 -0.95 -1.16 1.99 -1.58 -0.30 -3.38
Nucleus 0.80 1.70 -0.64 -1.25 -1.81 -1.92 -2.03 -1.50 -0.02 0.34 -0.51 1.32 -1.30 -1.02 -2.05
Peroxisomes 2.29 12.20 -0.89 -2.26 -6.66 -4.15 -2.86 -3.80 -1.56 0.70 -0.40 11.08 -3.10 -2.86 -7.91
Vacuole -0.48 -14.18 -10.76 -10.72 -5.58 -7.31 -5.46 -8.76 -7.45 -1.37 -6.93 -15.69 -13.89 -12.42 -7.09
Vacuole Periphery -0.25 -6.41 -4.42 -4.11 -1.29 -6.23 -3.04 -4.40 -2.10 -1.06 -5.21 -8.07 -6.34 -3.87 -2.02
Standard name
Human Ortholog
Description Protein involved in retrograde transport to the cis-Golgi network; forms heterodimer with Rgp1p that acts as a GTP exchange factor for Ypt6p; involved in transcription of rRNA and ribosomal protein genes
Localization
Cell Percentages Golgi (8%)
Cell Cycle Regulation No
Subcompartmental Group N/A

Ric1

Ric1


Nearest neighbours ( genes)

correlation threshold

0.6

1.0

0.80


Biological Process (Gene Ontology)
Molecular Function (Gene Ontology)
Cellular Component (Gene Ontology)

Endocytosis
Temp Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Vph1-GFP)
37℃
RT
Cell Cycle Omics

CYCLoPs (Ric1-GFP)
Gene / Allele Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Sac6-tdTomato)
Gene Images
Gene Images
Images are not yet available
Images are not yet available