Standard name
Human Ortholog
Description Cell wall protein of unknown function; localizes to the cytoplasm; deletion improves xylose fermentation in industrially engineered strains; YLR042C is not an essential gene
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Micrographs

ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 – – – – – –
Cell Periphery 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cytoplasm 0.97 0.84 0.87 0.82 0.88 0.74 – 0.66 0.57 0.62 0.57 0.55 0.96 0.98 0.98 0.94 0.93 0.95 0.73 0.34 0.39 0.14 0.2 0.29
Endoplasmic Reticulum 0 0.09 0.05 0 0 0 – 0 0 0 0 0 0 0 0 0.05 0.07 0.09 0.05 0.31 0.2 0.57 0.55 0.4
Endosome 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Golgi 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Mitochondria 0 0 0.13 0.32 0.1 0.45 – 0.47 0.62 0.58 0.64 0.6 0 0 0 0 0 0.05 0.06 0.13 0.22 0.1 0.05 0.05
Nucleus 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nuclear Periphery 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Vac/Vac Membrane 0 0.08 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0.07 0.07 0.07 0.08 0.07 0.12
ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 2 1 1 0 1 0 – 0 0 0 0 0 0 0 0 0 0 0 14 7 5 1 7 6
Bud 1 1 2 3 11 12 – 16 4 8 5 10 0 0 0 0 0 1 12 5 6 5 9 10
Bud Neck 3 0 0 0 0 0 – 4 1 1 1 1 0 0 0 0 0 0 11 4 1 1 3 2
Bud Site 0 0 0 0 1 2 – 1 0 3 1 4 0 0 0 0 0 0 – – – – – –
Cell Periphery 2 1 1 4 3 0 – 1 0 0 1 1 3 3 4 2 0 3 8 2 2 1 2 3
Cytoplasm 704 163 192 155 258 263 – 317 102 144 138 151 247 245 274 162 186 193 556 75 89 23 51 75
Endoplasmic Reticulum 17 18 10 1 3 2 – 0 0 1 0 0 4 3 1 9 13 18 39 67 45 94 138 102
Endosome 1 1 0 0 5 0 – 2 0 0 0 0 2 0 1 2 0 2 7 3 4 1 2 4
Golgi 0 3 1 1 0 0 – 0 0 0 0 0 1 0 0 1 2 1 0 3 1 1 0 1
Mitochondria 15 5 29 60 29 158 – 225 111 135 155 165 1 2 0 7 7 11 43 29 51 17 12 14
Nucleus 0 1 1 1 1 0 – 5 2 3 4 1 0 0 0 0 1 2 0 0 0 1 0 1
Nuclear Periphery 1 0 0 2 0 0 – 1 1 0 0 0 2 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 1 0 0 1 – 1 1 3 3 2 1 0 0 0 0 1 0 0 0 0 0 1
Peroxisomes 0 0 0 0 0 0 – 2 0 0 0 0 0 0 3 1 0 0 0 0 0 0 0 0
SpindlePole 0 0 1 1 2 0 – 10 2 5 7 12 0 0 0 2 0 0 7 1 2 1 0 0
Vac/Vac Membrane 0 15 1 2 5 5 – 4 2 3 4 1 0 0 0 5 8 7 51 15 15 12 17 30
Unique Cell Count 729 193 220 188 293 355 481 178 234 244 275 256 249 279 173 200 203 768 221 231 165 251 258
Labelled Cell Count 746 209 240 230 319 443 589 226 306 319 348 261 253 283 191 217 239 768 221 231 165 251 258


Endoplasmic Reticulum

(Huh et al. Nature 2003 425:686)
Screen WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 AF100 AF140 AF180
Mean Cell GFP Intensity (1e-4) 4.0 5.7 3.7 3.5 4.0 3.2 – 3.2 3.0 3.1 2.8 2.7 5.1 4.8 4.6 5.8 5.8 5.9 6.8 7.2 7.4
Std Deviation (1e-4) 0.6 1.4 1.0 1.5 1.4 1.4 – 1.3 1.9 1.6 1.6 1.2 1.2 1.1 0.9 2.0 4.5 2.4 1.5 1.6 1.7
Intensity Change (Log2) – – – -0.08 0.12 -0.21 – -0.2 -0.33 -0.26 -0.39 -0.44 0.45 0.37 0.31 0.64 0.65 0.67 0.87 0.96 1.0


Localization RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3
Cortical Patches 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0
Bud 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0
Cell Periphery 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0
Cytoplasm -1.4 0.3 -3.8 – -5.9 -6.8 -6.2 -7.3 -7.8 3.7 4.8 4.9 2.1 2.0 2.8
Endoplasmic Reticulum 0 0 0 – 0 0 0 0 0 -1.9 0 0 0.3 0.9 1.8
Endosome 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0
Golgi 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0
Mitochondria 0 0 0 – 0 0 0 0 0 -5.7 0 0 -3.1 -3.5 -2.7
Nucleus 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0
Nuclear Periphery 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0
Vacuole 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0

Images

Circle plot not available

Protein Concentration and Protein Localization Data

R1 R2 R3
G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase
Concentration 0.6656 1.4276 1.1878 1.1737 0.341 0.8935 1.1871 1.5552 1.1709 0.9556 0.3928 1.2181 1.0123 1.7534 1.4129 2.155 1.2714 1.2864
Actin 0.0151 0.0006 0.0101 0.008 0.002 0.0003 0.0312 0.0022 0.0251 0.0257 0.0013 0.004 0.0003 0.0002 0.0012 0.0198 0.0004 0.0005
Bud 0.0004 0.0001 0.0002 0.0011 0.0001 0 0.001 0.0048 0.0085 0.0034 0.0002 0.0043 0.0025 0.0001 0.0033 0.0001 0.0001 0.0007
Bud Neck 0.0006 0.0004 0.0005 0.0046 0.0004 0.0009 0.0032 0.0012 0.0022 0.0116 0.0004 0.0054 0.0001 0.0002 0.0009 0.0004 0.0002 0.0027
Bud Periphery 0.0017 0.0002 0.0005 0.0042 0.0001 0.0001 0.0017 0.0027 0.0055 0.0018 0.0002 0.0051 0.0008 0.0001 0.0011 0.0002 0.0001 0.0005
Bud Site 0.0009 0.0069 0.0018 0.0119 0.0001 0.0001 0.004 0.0263 0.0137 0.0128 0.0016 0.0006 0.0022 0.0014 0.0048 0.0001 0.0001 0.0001
Cell Periphery 0.0025 0.0013 0.0016 0.0026 0.0002 0.0002 0.0018 0.0018 0.0015 0.0015 0.0003 0.0006 0.0007 0.0006 0.0006 0.0018 0.0001 0.0002
Cytoplasm 0.3428 0.3886 0.4324 0.3944 0.2954 0.4966 0.3228 0.4307 0.3841 0.4886 0.4161 0.458 0.2438 0.3692 0.2985 0.2482 0.3406 0.3536
Cytoplasmic Foci 0.0148 0.0107 0.0205 0.011 0.0211 0.0098 0.0334 0.0129 0.0284 0.0136 0.0181 0.0128 0.0168 0.0193 0.0145 0.008 0.012 0.0093
Eisosomes 0.0004 0.0001 0.0002 0.0013 0.0003 0 0.0004 0.0002 0.0006 0.0002 0.0001 0.0001 0.0003 0.0001 0.0002 0.0006 0.0001 0.0001
Endoplasmic Reticulum 0.1465 0.1743 0.1606 0.1407 0.1487 0.1363 0.1998 0.2117 0.1772 0.0848 0.1663 0.1728 0.0564 0.0855 0.0763 0.037 0.0408 0.0233
Endosome 0.0293 0.016 0.0118 0.0194 0.0318 0.0172 0.0334 0.0239 0.027 0.0132 0.0145 0.0166 0.009 0.0217 0.0126 0.0033 0.0142 0.0063
Golgi 0.0061 0.0012 0.0017 0.0025 0.004 0.0006 0.0077 0.002 0.0063 0.0025 0.0021 0.002 0.0009 0.0019 0.0008 0.0025 0.0003 0.0002
Lipid Particles 0.0149 0.0092 0.009 0.0069 0.0457 0.0042 0.0158 0.0047 0.0111 0.0019 0.0046 0.0038 0.0022 0.0014 0.0015 0.0013 0.0005 0.0009
Mitochondria 0.0034 0.0004 0.002 0.0476 0.0015 0.0006 0.0133 0.0009 0.003 0.0017 0.0006 0.0036 0.0025 0.0008 0.0013 0.0003 0.0006 0.0006
None 0.3878 0.3649 0.3244 0.3018 0.4194 0.3198 0.2751 0.2508 0.2646 0.3121 0.3479 0.2837 0.6452 0.4827 0.5613 0.6685 0.5768 0.5902
Nuclear Periphery 0.0108 0.0115 0.0055 0.007 0.0073 0.005 0.0133 0.0063 0.0108 0.0077 0.005 0.0091 0.0044 0.0046 0.0049 0.0023 0.0051 0.0016
Nucleolus 0.0004 0.0003 0.0002 0.0032 0.0002 0.0001 0.0022 0.0007 0.0008 0.0005 0.0005 0.0006 0.0012 0.0004 0.0013 0.0001 0.0001 0.0002
Nucleus 0.0037 0.0034 0.0024 0.0045 0.0025 0.002 0.0092 0.0057 0.0071 0.0058 0.0027 0.0057 0.0026 0.0044 0.0058 0.0014 0.0028 0.0029
Peroxisomes 0.0069 0.0001 0.007 0.0013 0.0112 0.0002 0.0083 0.0004 0.0086 0.0009 0.0034 0.001 0.0003 0.0002 0.0004 0.0023 0.0002 0.0002
Punctate Nuclear 0.0039 0.0016 0.0027 0.0049 0.0045 0.0006 0.0128 0.0027 0.0059 0.0034 0.0118 0.0045 0.0039 0.001 0.0051 0.0006 0.0006 0.0042
Vacuole 0.0061 0.0076 0.0043 0.0148 0.0032 0.0048 0.008 0.007 0.0068 0.0055 0.002 0.0045 0.0036 0.0039 0.0031 0.0011 0.0038 0.0015
Vacuole Periphery 0.0011 0.0004 0.0004 0.0064 0.0006 0.0005 0.0016 0.0006 0.001 0.0007 0.0003 0.0012 0.0004 0.0004 0.0004 0.0002 0.0004 0.0002

Sequencing Data

R1 R2
G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Post-START S/G2 Metaphase Anaphase Telophase
Gene Expression – – – – – – – – – –
Translational Efficiency – – – – – – – – – –

Hit Data

Dataset Hit
Protein Concentration ✘
Protein Localization ✘
Gene Expression –
Translational Efficiency –

Micrographs

Cell Count

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
1693 2053 71 1483 1694 1967 1296 1578 3387 4020 1367 3061

Protein Abundance

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
Mean 594.55 736.30 816.53 845.35 670.06 722.72 754.08 819.65 632.32 729.66 757.32 832.10
Standard Deviation 81.77 105.14 116.04 163.23 217.09 93.49 111.40 156.66 168.34 99.84 112.51 160.39
Intensity Change Log 2 — 0.308496 0.457708 0.507751 — 0.109147 0.170427 0.290718 — 0.206307 0.312632 0.396830

Localization Score (DeepLoc)

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
Actin 0.000076 0.000572 0.000639 0.003553 0.000123 0.000288 0.000189 0.004907 0.000100 0.000433 0.000213 0.004251
Bud Neck 0.011114 0.052703 0.001088 0.014333 0.018895 0.043578 0.003655 0.016653 0.015006 0.048238 0.003522 0.015529
Bud Site 0.001970 0.017376 0.001841 0.063172 0.003154 0.011491 0.011189 0.076002 0.002562 0.014496 0.010703 0.069786
Cell Periphery 0.000182 0.000279 0.000314 0.001051 0.000126 0.000216 0.000208 0.001944 0.000154 0.000248 0.000213 0.001511
Cytoplasm 0.477583* 0.357966* 0.316521* 0.417113* 0.438920* 0.328496* 0.442705* 0.471623* 0.458246* 0.343546* 0.436152* 0.445214*
Cytoplasmic Foci 0.208843* 0.274029* 0.047872 0.063261 0.211290* 0.293191* 0.029536 0.067423 0.210067* 0.283405* 0.030489 0.065407
Eisosomes 0.000237 0.000254 0.000111 0.000130 0.000295 0.000333 0.000063 0.000204 0.000266 0.000293 0.000065 0.000168
Endoplasmic Reticulum 0.001272 0.003075 0.007234 0.023870 0.000850 0.005450 0.003954 0.026131 0.001061 0.004237 0.004124 0.025036
Endosome 0.009361 0.021668 0.003853 0.012519 0.007700 0.039374 0.002600 0.010082 0.008530 0.030331 0.002665 0.011263
Golgi 0.001470 0.009718 0.000144 0.006393 0.001970 0.011116 0.000248 0.003595 0.001720 0.010402 0.000243 0.004950
Lipid Particles 0.017509 0.014831 0.003574 0.007235 0.013913 0.029999 0.001820 0.006469 0.015711 0.022252 0.001912 0.006840
Mitochondria 0.003738 0.023649 0.000358 0.009462 0.006410 0.015660 0.002174 0.005306 0.005074 0.019740 0.002080 0.007320
Mitotic Spindle 0.001225 0.005224 0.000243 0.040097 0.001150 0.003908 0.004247 0.016228 0.001188 0.004580 0.004039 0.027792
None 0.011348 0.003104 0.004864 0.002053 0.002397 0.002282 0.004604 0.001619 0.006872 0.002701 0.004618 0.001829
Nuclear Periphery 0.000638 0.001079 0.000803 0.001411 0.000610 0.001072 0.002063 0.000775 0.000624 0.001076 0.001998 0.001083
Nuclear Periphery Foci 0.000591 0.000535 0.003402 0.003174 0.000345 0.000648 0.001181 0.002084 0.000468 0.000590 0.001296 0.002612
Nucleolus 0.002101 0.001856 0.001407 0.000493 0.000747 0.002143 0.000473 0.000367 0.001423 0.001996 0.000522 0.000428
Nucleus 0.210575* 0.113710 0.545031* 0.125447 0.241191* 0.094899 0.436204* 0.142618* 0.225888* 0.104506 0.441856* 0.134299*
Peroxisomes 0.003833 0.019338 0.000684 0.003413 0.005675 0.014197 0.001181 0.007567 0.004755 0.016823 0.001155 0.005554
Vacuole 0.035642 0.076129 0.059739 0.196012 0.043619 0.098814 0.051347 0.136977 0.039632 0.087229 0.051783 0.165579
Vacuole Periphery 0.000689 0.002904 0.000277 0.005807 0.000620 0.002848 0.000357 0.001427 0.000654 0.002877 0.000353 0.003549

Localization Changes (T-score)

R1 R2 R1 & R2
UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14 UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14 UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14
Actin -7.55 -4.75 -3.66 -2.87 -2.52 -4.64 -2.02 -4.87 -4.64 -4.74 -8.83 -3.29 -6.09 -5.40 -5.78
Bud Neck -16.21 12.17 -0.06 15.78 -9.89 -10.00 11.49 2.87 12.20 -8.46 -18.61 13.21 2.23 19.82 -10.34
Bud Site -12.79 -0.33 -12.58 -7.60 -12.54 -6.89 -3.37 -14.08 -11.73 -11.20 -13.93 -3.76 -18.85 -13.80 -13.28
Cell Periphery -1.92 -3.67 -3.07 -2.37 -2.05 -5.52 -4.95 -3.18 -2.96 -3.00 -3.51 -3.46 -4.03 -3.60 -3.79
Cytoplasm 14.24 5.39 8.67 -3.74 -2.95 13.21 2.17 -1.23 -13.14 -3.08 19.32 5.32 5.20 -11.86 -1.32
Cytoplasmic Foci -9.15 16.89 30.15 39.64 -0.26 -11.82 35.69 27.17 41.67 -10.34 -14.76 48.18 40.39 57.23 -10.78
Eisosomes -0.76 5.10 9.35 8.56 1.10 -1.45 11.83 4.76 6.63 -6.17 -1.56 16.82 8.49 10.13 -7.20
Endoplasmic Reticulum -2.33 -4.16 -5.45 -4.71 -2.07 -5.94 -3.59 -6.86 -4.97 -5.38 -5.82 -3.65 -8.74 -6.87 -6.39
Endosome -10.43 5.41 0.60 9.87 -4.06 -18.96 7.59 -0.30 18.14 -6.71 -21.28 11.38 0.26 20.24 -9.05
Golgi -11.04 8.85 -2.39 5.16 -3.92 -9.90 7.83 -1.01 8.22 -4.42 -14.66 10.26 -2.57 9.41 -5.58
Lipid Particles 1.88 10.34 8.34 7.16 -1.23 -8.86 13.69 7.95 14.50 -5.33 -5.38 18.54 11.46 15.73 -5.98
Mitochondria -12.07 6.30 -1.36 8.72 -4.00 -6.15 3.79 1.83 8.42 -2.04 -13.04 3.96 0.26 12.02 -3.09
Mitotic Spindle -3.28 1.50 -8.77 -7.44 -9.23 -2.73 -2.17 -5.98 -4.56 -4.25 -4.27 -2.21 -10.58 -8.67 -8.36
None 5.99 4.08 7.10 2.71 2.01 0.22 -2.49 1.56 1.54 5.91 5.41 3.39 7.13 3.05 5.82
Nuclear Periphery -1.29 -2.15 -8.53 -1.93 -5.46 -2.51 -2.18 -5.15 -0.04 1.51 -2.28 -2.19 -9.89 -1.66 0.97
Nuclear Periphery Foci 0.49 -3.50 -3.96 -4.38 1.31 -1.52 -3.70 -5.98 -5.99 -2.24 -0.72 -4.25 -6.43 -6.60 -2.54
Nucleolus 0.81 2.44 7.46 5.40 3.34 -6.39 4.43 5.52 8.38 1.91 -2.69 7.85 8.73 9.40 2.29
Nucleus 17.41 -9.22 9.73 -5.70 10.92 26.48 -21.65 12.36 -11.34 30.25 30.87 -26.02 15.58 -11.96 34.23
Peroxisomes -13.25 7.32 2.93 14.84 -4.70 -10.00 8.69 -0.03 7.39 -4.96 -16.53 9.60 1.16 15.44 -5.62
Vacuole -14.17 -2.98 -32.06 -25.41 -13.58 -15.56 -6.60 -24.52 -14.03 -17.33 -20.92 -8.30 -39.85 -28.11 -26.82
Vacuole Periphery -4.85 3.76 -3.18 -0.54 -3.69 -14.31 2.89 -1.91 6.64 -3.10 -9.07 3.37 -3.58 1.36 -4.33
Standard name
Human Ortholog
Description Cell wall protein of unknown function; localizes to the cytoplasm; deletion improves xylose fermentation in industrially engineered strains; YLR042C is not an essential gene
Localization
Cell Percentages —
Cell Cycle Regulation No
Subcompartmental Group N/A

Nfg1

Nfg1


Nearest neighbours ( genes)

correlation threshold

0.6

1.0

0.80


Biological Process (Gene Ontology)
Molecular Function (Gene Ontology)
Cellular Component (Gene Ontology)

Endocytosis
Temp Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Vph1-GFP)
37℃
RT
Cell Cycle Omics

CYCLoPs (Nfg1-GFP)
Gene / Allele Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Sac6-tdTomato)
Gene Images
Gene Images
Images are not yet available
Images are not yet available