Standard name
Human Ortholog
Description Major of three pyruvate decarboxylase isozymes; key enzyme in alcoholic fermentation; decarboxylates pyruvate to acetaldehyde; involved in amino acid catabolism; subject to glucose-, ethanol-, and autoregulation; activated by phosphorylation in response to glucose levels; N-terminally propionylated in vivo
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Micrographs

ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches – 0 – 0 0 0 0 – 0 0 0 0 – 0 – – – – 0 0 0 0 0 0
Bud – 0 – 0 0 0 0 – 0 0 0 0 – 0 – – – – 0 0 0 0 0 0
Bud Neck – 0 – 0 0 0 0 – 0 0 0 0 – 0 – – – – 0 0 0 0 0 0
Bud Site – 0 – 0 0 0 0 – 0 0 0 0 – 0 – – – – – – – – – –
Cell Periphery – 0.18 – 0.65 0.6 0.49 0.53 – 0.69 0.41 0.52 0.65 – 0.5 – – – – 0.3 0.4 0.29 0.25 0.35 0.25
Cytoplasm – 0.94 – 0.74 0.55 0.64 0.6 – 0.43 0.68 0.48 0.5 – 0.88 – – – – 0.67 0.52 0.59 0.63 0.5 0.58
Endoplasmic Reticulum – 0 – 0 0 0 0 – 0 0 0 0 – 0 – – – – 0 0 0 0.07 0 0
Endosome – 0 – 0 0 0 0 – 0 0 0 0 – 0 – – – – 0 0 0 0 0 0
Golgi – 0 – 0 0 0 0 – 0 0 0 0 – 0 – – – – 0 0 0 0 0 0
Mitochondria – 0 – 0 0 0 0 – 0 0 0 0 – 0 – – – – 0 0 0 0 0 0
Nucleus – 0 – 0 0 0 0 – 0 0 0 0 – 0 – – – – 0 0 0 0 0 0.08
Nuclear Periphery – 0 – 0 0 0 0 – 0 0 0 0 – 0 – – – – 0 0 0 0 0 0
Nucleolus – 0 – 0 0 0 0 – 0 0 0 0 – 0 – – – – 0 0 0 0 0 0
Peroxisomes – 0 – 0 0 0 0 – 0 0 0 0 – 0 – – – – 0 0 0 0 0 0
SpindlePole – 0 – 0 0 0 0 – 0 0 0 0 – 0 – – – – 0 0 0 0 0 0
Vac/Vac Membrane – 0 – 0 0.12 0 0 – 0 0.05 0 0 – 0 – – – – 0 0 0 0 0 0
ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches – 0 – 0 0 0 0 – 0 0 0 0 – 0 – – – – 0 0 0 0 0 0
Bud – 0 – 0 0 0 0 – 0 0 0 0 – 0 – – – – 0 0 0 0 0 0
Bud Neck – 0 – 0 0 0 0 – 0 0 0 0 – 0 – – – – 0 0 0 0 0 0
Bud Site – 0 – 0 0 0 0 – 0 0 0 0 – 0 – – – – – – – – – –
Cell Periphery – 3 – 50 36 34 30 – 37 15 12 13 – 8 – – – – 19 19 19 5 17 11
Cytoplasm – 16 – 57 33 45 34 – 23 25 11 10 – 14 – – – – 42 26 39 14 24 27
Endoplasmic Reticulum – 0 – 0 0 0 0 – 1 0 0 0 – 0 – – – – 0 0 0 1 0 0
Endosome – 0 – 0 0 0 0 – 0 0 0 0 – 0 – – – – 0 0 0 0 0 0
Golgi – 0 – 0 2 1 0 – 0 0 0 0 – 0 – – – – 0 0 0 0 0 0
Mitochondria – 0 – 0 0 0 0 – 0 0 0 0 – 0 – – – – 0 0 0 0 0 0
Nucleus – 0 – 0 0 0 0 – 0 0 0 0 – 0 – – – – 0 1 2 0 2 3
Nuclear Periphery – 0 – 0 0 0 0 – 0 0 0 0 – 0 – – – – 0 0 1 0 0 0
Nucleolus – 0 – 0 0 0 0 – 0 0 1 0 – 0 – – – – 0 0 0 0 0 0
Peroxisomes – 0 – 0 0 0 0 – 0 0 0 0 – 0 – – – – 0 0 0 0 0 0
SpindlePole – 0 – 0 0 0 0 – 0 0 0 0 – 0 – – – – 0 0 0 0 0 0
Vac/Vac Membrane – 0 – 0 7 0 0 – 1 2 0 0 – 0 – – – – 0 0 0 0 0 0
Unique Cell Count 17 77 60 70 57 54 37 23 20 16 64 50 67 23 50 47
Labelled Cell Count 19 107 78 80 64 62 42 24 23 22 64 50 67 23 50 47


Ambiguous, Cytoplasm, Nucleus

(Huh et al. Nature 2003 425:686)
Screen WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 AF100 AF140 AF180
Mean Cell GFP Intensity (1e-4) – 829.2 – 765.0 632.9 818.7 762.5 – 771.5 757.5 771.6 762.6 – 721.2 – – – – – – –
Std Deviation (1e-4) – 140.3 – 153.4 251.7 206.5 114.3 – 142.1 204.6 127.4 123.4 – 106.5 – – – – – – –
Intensity Change (Log2) – – – – – – – – – – – – – – – – – – – – –


Localization RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3
Actin – – – – – – – – – – – – – – –
Bud – – – – – – – – – – – – – – –
Bud Neck – – – – – – – – – – – – – – –
Bud Site – – – – – – – – – – – – – – –
Cell Periphery – – – – – – – – – – – – – – –
Cyto – – – – – – – – – – – – – – –
Endoplasmic Reticulum – – – – – – – – – – – – – – –
Endosome – – – – – – – – – – – – – – –
Golgi – – – – – – – – – – – – – – –
Mitochondria – – – – – – – – – – – – – – –
Nuclear Periphery – – – – – – – – – – – – – – –
Nuc – – – – – – – – – – – – – – –
Nucleolus – – – – – – – – – – – – – – –
Peroxisomes – – – – – – – – – – – – – – –
SpindlePole – – – – – – – – – – – – – – –
Vac – – – – – – – – – – – – – – –
Cortical Patches – – – – – – – – – – – – – – –
Cytoplasm – – – – – – – – – – – – – – –
Nucleus – – – – – – – – – – – – – – –
Vacuole – – – – – – – – – – – – – – –

Images

Circle plot not available

Protein Concentration and Protein Localization Data

R1 R2 R3
G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase
Concentration – – – – – – – – – – – – – – – – – –
Actin 0.0001 0.0002 0.0003 0.0007 0.0002 0.0005 0.0014 0.0014 0.0015 0.0015 0.0029 0.0004 0.0046 0.0007 0.0147 0.0003 0.0071 0.0003
Bud 0 0.0001 0.0002 0.0001 0.0003 0.0001 0.0004 0.0011 0.0005 0.0005 0.0003 0.0002 0.003 0.0068 0.0011 0.0006 0.0012 0.0004
Bud Neck 0 0 0 0 0.0002 0.0001 0.0001 0.0001 0.0003 0 0.0002 0.0001 0.0004 0.0001 0.0002 0.0002 0.0008 0.0002
Bud Periphery 0 0 0 0 0.0001 0 0.0003 0.0006 0.0003 0.0002 0.0003 0.0001 0.0024 0.0014 0.0008 0.0009 0.002 0.0002
Bud Site 0 0 0.0001 0 0 0 0.0012 0.0003 0.0002 0.0002 0.0003 0.0001 0.003 0.0057 0.0018 0.0002 0.0016 0.0001
Cell Periphery 0 0.0002 0.0003 0.0002 0.0007 0.0001 0.0039 0.0013 0.0034 0.0009 0.0063 0.0002 0.0033 0.0038 0.0016 0.0012 0.0064 0.0005
Cytoplasm 0.1183 0.4273 0.1995 0.0502 0.1099 0.0337 0.0667 0.2909 0.0519 0.0159 0.0105 0.0119 0.0404 0.3526 0.0039 0.0029 0.0031 0.0879
Cytoplasmic Foci 0 0 0 0 0.0001 0 0.0112 0.0001 0.0026 0 0.0025 0.0018 0.0115 0.0005 0.037 0.0669 0.0032 0.0006
Eisosomes 0 0 0.0001 0 0 0 0.0161 0.0002 0.0016 0.0003 0.0043 0.0001 0.0013 0.0003 0.0055 0.001 0.0052 0.0001
Endoplasmic Reticulum 0 0.0001 0.0001 0.0001 0.0002 0 0.0001 0.0003 0.0003 0.0002 0.0006 0 0.0011 0.0003 0.0005 0.0001 0.0003 0
Endosome 0 0 0 0 0.0002 0 0.0002 0.0001 0.0004 0.0001 0.0034 0 0.0042 0 0.0057 0.0003 0.0019 0
Golgi 0 0 0 0 0 0 0.0003 0 0.0004 0 0.0026 0 0.0007 0 0.0055 0.0001 0.0026 0
Lipid Particles 0 0 0 0 0 0 0.0032 0 0.0032 0 0.0105 0.0001 0.0049 0.0002 0.0148 0.0035 0.0184 0.0001
Mitochondria 0.0031 0.0099 0.0227 0.011 0.0171 0.0056 0.0707 0.0289 0.0421 0.0444 0.0508 0.0107 0.0583 0.0168 0.1147 0.0995 0.0966 0.0203
None 0.0002 0.0005 0.0013 0.0008 0.0006 0.0002 0.0093 0.0013 0.0037 0.002 0.0076 0.0011 0.0397 0.0037 0.0121 0.0363 0.0095 0.001
Nuclear Periphery 0.0731 0.0559 0.1291 0.2819 0.181 0.109 0.0554 0.0883 0.1317 0.1354 0.2163 0.0615 0.0589 0.048 0.0318 0.0189 0.0315 0.0308
Nucleolus 0.0336 0.0213 0.0474 0.0334 0.0082 0.017 0.3664 0.0777 0.2114 0.0437 0.4705 0.1366 0.3474 0.0279 0.3589 0.4868 0.3571 0.1491
Nucleus 0.771 0.4757 0.5912 0.6176 0.6129 0.8286 0.3691 0.4854 0.5113 0.7412 0.1141 0.7628 0.3511 0.5258 0.3644 0.2497 0.3809 0.6976
Peroxisomes 0 0 0.0001 0 0.0001 0 0.0014 0.0002 0.0004 0.0001 0.0006 0.0001 0.0012 0.0001 0.0035 0.0026 0.0012 0.0002
Punctate Nuclear 0 0.0001 0.0003 0.0002 0.0001 0.0001 0.0037 0.0002 0.0019 0.0002 0.0069 0.0032 0.0286 0.0005 0.008 0.0032 0.0022 0.0002
Vacuole 0.0001 0.0009 0.0011 0.0003 0.0095 0.0003 0.0039 0.0049 0.0069 0.0015 0.0117 0.0008 0.0148 0.0021 0.0028 0.0045 0.0113 0.0015
Vacuole Periphery 0.0005 0.0076 0.0062 0.0034 0.0586 0.0047 0.0149 0.0167 0.0239 0.0116 0.077 0.0082 0.0192 0.0027 0.0106 0.0203 0.0558 0.0089

Sequencing Data

R1 R2
G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Post-START S/G2 Metaphase Anaphase Telophase
Gene Expression 10610.4858 11961.2685 14296.0664 12291.003 12403.2885 11875.236 13502.6284 12682.7842 14456.8009 12040.0934
Translational Efficiency 1.2948 1.256 1.1625 1.1445 1.169 1.2333 1.3659 1.3519 1.2544 1.4974

Hit Data

Dataset Hit
Protein Concentration –
Protein Localization ✘
Gene Expression ✘
Translational Efficiency ✘

Micrographs

Cell Count

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
178 165 1620 528 728 1406 1211 945 906 1571 2831 1473

Protein Abundance

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
Mean 51469.41 53486.43 48347.29 50091.35 51805.37 47890.80 48637.61 49298.04 51739.36 48478.50 48471.48 49582.40
Standard Deviation 6380.71 4961.57 5422.70 7879.61 5334.47 8580.30 5221.91 8378.31 5557.20 8450.91 5339.66 8211.85
Intensity Change Log 2 — 0.055458 -0.090280 -0.039154 — -0.113353 -0.091029 -0.071571 — -0.026754 -0.090656 -0.055324

Localization Score (DeepLoc)

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
Actin 0.000009 0.000030 0.000027 0.000472 0.000012 0.000131 0.000078 0.000163 0.000011 0.000121 0.000049 0.000274
Bud Neck 0.000099 0.000503 0.000128 0.000890 0.000072 0.000622 0.000576 0.000785 0.000077 0.000610 0.000320 0.000822
Bud Site 0.000014 0.000045 0.000014 0.000967 0.000026 0.000489 0.000200 0.001170 0.000024 0.000442 0.000094 0.001097
Cell Periphery 0.274430* 0.239937* 0.197501* 0.314039* 0.183761* 0.147755* 0.161675* 0.265423* 0.201575* 0.157437* 0.182176* 0.282850*
Cytoplasm 0.004990 0.045271 0.029818 0.073465 0.032576 0.119501* 0.097794 0.085295 0.027156 0.111705* 0.058895 0.081055
Cytoplasmic Foci 0.000003 0.000059 0.000015 0.000597 0.000009 0.001160 0.000044 0.000263 0.000008 0.001044 0.000028 0.000382
Eisosomes 0.000094 0.000067 0.000011 0.000055 0.000019 0.000016 0.000021 0.000033 0.000034 0.000022 0.000015 0.000041
Endoplasmic Reticulum 0.000153 0.000048 0.000188 0.000055 0.000038 0.000244 0.000064 0.000063 0.000060 0.000223 0.000135 0.000060
Endosome 0.000003 0.000006 0.000005 0.000129 0.000002 0.000017 0.000006 0.000035 0.000002 0.000016 0.000005 0.000069
Golgi 0.000030 0.000472 0.000098 0.000082 0.000151 0.000218 0.000060 0.000058 0.000127 0.000244 0.000082 0.000067
Lipid Particles 0.000241 0.000667 0.000429 0.000690 0.000523 0.001539 0.002218 0.000572 0.000468 0.001447 0.001194 0.000614
Mitochondria 0.000001 0.000037 0.000005 0.000336 0.000002 0.000266 0.000003 0.000225 0.000001 0.000242 0.000004 0.000265
Mitotic Spindle 0.000010 0.000010 0.000004 0.000352 0.000008 0.000022 0.000006 0.000890 0.000008 0.000020 0.000005 0.000697
None 0.000000 0.000006 0.000001 0.000106 0.000001 0.001126 0.000002 0.000032 0.000001 0.001008 0.000001 0.000059
Nuclear Periphery 0.000135 0.000790 0.000340 0.000068 0.000412 0.000391 0.000256 0.000093 0.000358 0.000432 0.000304 0.000084
Nuclear Periphery Foci 0.000080 0.000451 0.000165 0.000127 0.000191 0.000523 0.000390 0.000154 0.000169 0.000515 0.000261 0.000145
Nucleolus 0.000008 0.000039 0.000008 0.000665 0.000007 0.000309 0.000019 0.001848 0.000007 0.000281 0.000013 0.001424
Nucleus 0.000057 0.001633 0.000430 0.000934 0.000220 0.004279 0.001217 0.001123 0.000188 0.004001 0.000766 0.001055
Peroxisomes 0.000008 0.000002 0.000003 0.000274 0.000005 0.000020 0.000007 0.000623 0.000005 0.000019 0.000005 0.000498
Vacuole 0.718556* 0.706597* 0.769678* 0.605354* 0.780957* 0.719115* 0.734590* 0.640375* 0.768698* 0.717800* 0.754669* 0.627822*
Vacuole Periphery 0.001079 0.003329 0.001133 0.000343 0.001009 0.002257 0.000775 0.000775 0.001023 0.002370 0.000980 0.000620

Localization Changes (T-score)

R1 R2 R1 & R2
UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14 UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14 UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14
Actin -2.21 -6.80 -1.77 -1.68 -1.70 -4.97 -11.36 -3.76 -1.13 -2.58 -5.06 -12.26 -2.97 -1.67 -2.60
Bud Neck -1.71 -1.06 -2.02 -0.89 -1.93 -3.63 -8.21 -3.55 -0.89 -1.77 -3.83 -7.47 -4.03 -0.96 -3.01
Bud Site -1.46 -0.18 -2.49 -2.42 -2.49 -2.56 -3.63 -3.32 -2.01 -2.95 -2.58 -3.02 -4.06 -2.30 -3.86
Cell Periphery 1.70 4.80 -1.93 -4.04 -9.17 5.41 3.39 -7.85 -13.15 -11.37 6.68 3.19 -8.87 -16.29 -13.47
Cytoplasm -4.42 -12.72 -10.46 -2.55 -6.35 -15.21 -13.08 -9.06 5.26 2.28 -16.56 -9.83 -11.49 5.48 -4.71
Cytoplasmic Foci -1.77 -1.41 -1.53 -1.39 -1.49 -1.95 -2.23 -2.02 1.45 -1.70 -1.96 -2.32 -2.37 1.18 -2.22
Eisosomes 1.01 3.56 0.94 0.19 -2.03 0.62 -0.62 -2.40 -2.75 -2.09 2.06 3.48 -1.10 -2.31 -3.03
Endoplasmic Reticulum 0.93 -0.44 0.84 -0.29 3.28 -4.55 -1.40 -1.36 3.27 -0.33 -3.65 -2.61 -0.40 3.54 2.46
Endosome -1.08 -1.61 -1.06 -1.03 -1.04 -3.18 -6.23 -1.38 -0.78 -1.25 -3.23 -5.38 -1.51 -1.18 -1.44
Golgi -2.13 -3.98 -0.78 1.82 0.24 -1.53 1.69 1.36 3.46 -0.14 -2.54 0.95 0.99 3.71 0.35
Lipid Particles -2.90 -1.76 -1.75 -0.35 -1.14 -5.86 -10.88 -0.99 4.79 9.31 -6.30 -8.79 -1.68 4.06 4.13
Mitochondria -1.38 -2.40 -1.53 -1.36 -1.51 -3.85 -2.11 -2.34 -0.08 -2.33 -3.90 -2.33 -2.72 -0.45 -2.70
Mitotic Spindle -0.57 4.27 -0.99 -0.99 -1.01 -2.35 3.03 -1.37 -1.35 -1.38 -2.33 6.92 -1.60 -1.56 -1.60
None -1.98 -2.42 -1.13 -1.07 -1.12 -2.87 -3.73 -1.83 2.75 -1.79 -2.87 -2.68 -1.77 2.65 -1.75
Nuclear Periphery -1.96 -7.71 2.99 2.12 12.25 0.06 2.54 5.16 7.47 10.57 -1.37 1.04 5.48 6.83 14.87
Nuclear Periphery Foci -1.78 -3.01 -1.60 1.52 0.96 -6.73 -5.88 1.00 7.21 6.55 -7.02 -4.40 0.58 7.28 4.98
Nucleolus -1.30 -0.03 -1.43 -1.37 -1.43 -2.45 -9.12 -1.93 -1.49 -1.92 -2.47 -5.82 -2.29 -1.68 -2.28
Nucleus -1.99 -4.28 -1.25 0.58 -0.73 -4.71 -7.56 -2.77 3.38 -0.44 -4.86 -7.36 -2.86 3.57 -1.27
Peroxisomes 1.19 0.98 -1.06 -1.08 -1.08 -2.51 -2.17 -1.72 -1.67 -1.71 -2.33 0.41 -1.99 -1.93 -1.99
Vacuole 0.57 -3.15 5.71 5.19 12.61 7.87 5.87 13.35 7.29 9.69 6.94 2.09 15.33 9.75 16.45
Vacuole Periphery -1.56 -0.24 1.70 2.02 4.25 -2.42 0.57 0.17 2.49 -0.30 -2.83 0.01 0.75 3.46 1.07
Standard name
Human Ortholog
Description Major of three pyruvate decarboxylase isozymes; key enzyme in alcoholic fermentation; decarboxylates pyruvate to acetaldehyde; involved in amino acid catabolism; subject to glucose-, ethanol-, and autoregulation; activated by phosphorylation in response to glucose levels; N-terminally propionylated in vivo
Localization
Cell Percentages cytoplasm (91%)
Cell Cycle Regulation No
Subcompartmental Group N/A

Pdc1

Pdc1


Nearest neighbours ( genes)

correlation threshold

0.6

1.0

0.80


Biological Process (Gene Ontology)
Molecular Function (Gene Ontology)
Cellular Component (Gene Ontology)

Endocytosis
Temp Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Vph1-GFP)
37℃
RT
Cell Cycle Omics

CYCLoPs (Pdc1-GFP)
Gene / Allele Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Sac6-tdTomato)
Gene Images
Gene Images
Images are not yet available
Images are not yet available