Standard name
Human Ortholog
Description Putative protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to the endoplasmic reticulum; YLR050C is not an essential gene

Micrographs

ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0.17 0.21 0.13 0 0 0 0 0 0
Bud 0 0 0 0 0 0 0 0 0 0.05 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cell Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0.07 0.17 0.13 0.15 0 0 0 0 0 0
Cytoplasm 0.6 0.4 0.5 0.44 0.62 0.44 0.39 0.36 0.41 0.43 0.4 0.54 0.64 0.72 0.27 0.27 0.26 0.19 0.09 0.1 0.12 0.17 0.19
Endoplasmic Reticulum 0.21 0.56 0.26 0.34 0.19 0.25 0.21 0.22 0.17 0.17 0.14 0.45 0.37 0.24 0 0 0 0.61 0.7 0.65 0.6 0.55 0.51
Endosome 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Golgi 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0.19 0.13 0.17 0 0 0 0 0 0
Mitochondria 0.06 0 0.27 0.46 0.18 0.48 0.44 0.55 0.47 0.52 0.53 0 0 0 0.06 0.07 0.05 0.06 0.05 0.12 0.12 0.11 0.06
Nucleus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nuclear Periphery 0 0 0 0 0 0 0 0.05 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Vac/Vac Membrane 0.18 0.09 0.07 0 0.1 0.05 0.08 0.07 0.11 0.05 0.06 0 0 0 0.29 0.31 0.38 0 0 0 0 0.06 0.08
ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 5 6 1 0 2 0 2 2 2 3 3 0 1 1 45 38 31 8 4 8 4 8 9
Bud 3 3 5 2 3 6 10 11 9 16 16 0 0 0 2 2 3 13 3 0 6 4 11
Bud Neck 0 0 1 0 1 0 0 0 0 0 0 1 0 0 0 0 0 8 2 1 1 1 3
Bud Site 1 0 2 0 0 0 2 0 1 0 2 0 0 0 0 0 1
Cell Periphery 0 4 1 5 6 8 5 6 9 6 18 7 6 17 45 23 36 4 0 2 1 2 1
Cytoplasm 263 87 96 54 121 117 144 125 163 144 178 96 98 164 69 48 62 110 23 22 24 49 53
Endoplasmic Reticulum 91 122 49 42 38 65 77 76 67 57 60 80 57 54 3 8 5 349 180 148 121 161 142
Endosome 9 3 1 0 8 0 4 3 7 2 5 1 0 1 10 1 3 6 5 1 1 3 7
Golgi 1 0 0 0 0 0 0 0 0 0 0 0 0 1 48 23 40 5 2 0 2 2 4
Mitochondria 24 1 51 56 35 126 161 194 186 175 236 2 3 2 15 13 12 34 13 27 23 33 17
Nucleus 2 1 1 0 0 0 6 1 2 1 3 0 1 0 2 4 5 1 0 0 0 0 0
Nuclear Periphery 1 1 1 1 0 3 10 19 11 2 8 1 0 0 0 1 2 3 3 4 2 2 0
Nucleolus 0 0 0 0 0 0 0 0 0 1 0 0 0 0 0 1 1 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 1 0 0 0 0 0 0 0 2 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 1 1 1 1 1 0 0 0 2 1 2 4 0 1 0 2 1
Vac/Vac Membrane 79 20 13 0 20 14 29 26 43 17 27 2 1 5 75 56 88 19 8 4 7 17 21
Unique Cell Count 435 216 192 123 196 265 369 350 398 337 444 177 154 227 258 179 234 576 256 229 202 295 281
Labelled Cell Count 479 248 222 160 234 339 452 464 501 425 557 190 167 245 318 219 291 576 256 229 202 295 281


Endoplasmic Reticulum

(Huh et al. Nature 2003 425:686)
Screen WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 AF100 AF140 AF180
Mean Cell GFP Intensity (1e-4) 5.6 7.2 4.5 4.9 5.8 4.7 5.0 4.6 4.9 4.8 4.8 6.4 6.8 6.4 11.6 11.1 11.0 6.5 6.6 7.0
Std Deviation (1e-4) 1.2 1.6 1.1 1.1 2.1 1.3 1.7 1.5 1.4 1.7 1.6 1.4 1.6 1.5 2.7 2.5 2.4 1.7 1.8 1.9
Intensity Change (Log2) 0.11 0.37 0.07 0.15 0.02 0.12 0.11 0.08 0.51 0.61 0.52 1.37 1.3 1.3 0.54 0.55 0.65


Localization RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3
Cortical Patches 0 0 0 0 0 0 0 0 0 0 0 5.9 6.5 5.0
Bud 0 0 0 0.1 0 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cell Periphery 0 0 0 0 0 0 0 0 0 0 3.5 5.9 4.8 5.4
Cytoplasm -1.1 2.3 -1.2 -2.5 -3.2 -2.1 -1.6 -2.3 0.8 2.5 4.7 -5.1 -4.6 -5.0
Endoplasmic Reticulum 0 -1.4 0 -1.3 0 -2.5 0 0 4.0 2.3 -0.4 -8.0 -5.6 -7.2
Endosome 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Golgi 0 0 0 0 0 0 0 0 0 0 0 6.3 5.1 6.0
Mitochondria 0 -2.1 0 4.0 0 4.7 0 0 -7.0 -6.3 -7.9 -6.2 -4.9 -6.2
Nucleus 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nuclear Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Vacuole -2.9 1.2 -0.7 0.5 0.3 1.6 -0.8 -0.3 -2.7 -2.9 -2.3 5.9 6.1 7.4

Images

Circle plot not available

Protein Concentration and Protein Localization Data

R1 R2 R3
G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase
Concentration 2.2053 2.6698 2.2548 2.4825 2.0476 2.5568 2.5992 2.235 2.6879 1.7503 2.3712 1.7933 3.5466 3.5868 3.4065 3.353 3.0762 3.3758
Actin 0.0091 0.0024 0.0138 0.0018 0.011 0.0009 0.0338 0.0035 0.0101 0.0024 0.0155 0.0013 0.0272 0.0005 0.0098 0.0011 0.0009 0.0015
Bud 0.0002 0.0002 0.0008 0.0001 0.0003 0 0.0014 0.0022 0.0012 0.0003 0.0363 0.0001 0.0002 0.0001 0.0001 0.0014 0 0
Bud Neck 0.0002 0.0008 0.0015 0.0003 0.0006 0.0005 0.003 0.0003 0.0009 0.0167 0.0028 0.0076 0.0011 0.0002 0.0008 0.0006 0.0005 0.0018
Bud Periphery 0.0003 0.0011 0.0014 0.0002 0.0008 0.0001 0.0012 0.0006 0.0013 0.0004 0.0189 0.0002 0.0004 0.0001 0.0003 0.0007 0 0.0001
Bud Site 0.0006 0.0044 0.0109 0.0001 0.0011 0.0001 0.0079 0.009 0.0152 0.0051 0.0045 0.0001 0.0009 0.0105 0.0008 0.0003 0 0.0001
Cell Periphery 0.0008 0.0024 0.0014 0.0008 0.0003 0.0004 0.0012 0.0023 0.002 0.0004 0.0012 0.0006 0.001 0.0027 0.0019 0.0018 0.0003 0.0004
Cytoplasm 0.1307 0.2514 0.1685 0.1097 0.0965 0.1912 0.1434 0.2406 0.1867 0.1742 0.1835 0.2486 0.14 0.2631 0.2546 0.2175 0.2016 0.2488
Cytoplasmic Foci 0.0128 0.006 0.0156 0.0169 0.0137 0.0056 0.0225 0.01 0.0303 0.0111 0.0205 0.0067 0.0056 0.0052 0.0136 0.0055 0.0031 0.0083
Eisosomes 0.0002 0.0002 0.0002 0.0001 0.001 0 0.0009 0.0001 0.0005 0.0001 0.0005 0 0.0006 0.0001 0.0002 0.0001 0.0001 0
Endoplasmic Reticulum 0.6161 0.5797 0.6313 0.6916 0.5517 0.6614 0.5166 0.5562 0.5768 0.6566 0.3115 0.6391 0.661 0.5624 0.5636 0.6368 0.6387 0.6083
Endosome 0.0348 0.0167 0.0218 0.0262 0.0274 0.0167 0.0483 0.0251 0.0223 0.0213 0.0659 0.0108 0.0195 0.0152 0.0214 0.0126 0.0114 0.0155
Golgi 0.0041 0.0012 0.0049 0.0078 0.0084 0.0014 0.0096 0.0031 0.0052 0.0049 0.0218 0.0005 0.0065 0.0008 0.0034 0.0012 0.0016 0.0007
Lipid Particles 0.0218 0.0058 0.016 0.0632 0.0192 0.0063 0.032 0.0104 0.0168 0.0074 0.0044 0.0032 0.0147 0.0074 0.0086 0.0084 0.0065 0.0044
Mitochondria 0.0085 0.0098 0.0058 0.0343 0.0436 0.0019 0.0123 0.008 0.0162 0.0011 0.0189 0.0005 0.0028 0.0003 0.0011 0.001 0.0003 0.0006
None 0.093 0.0884 0.0674 0.0195 0.1188 0.0771 0.078 0.0816 0.0555 0.0628 0.1218 0.0466 0.0708 0.0813 0.0928 0.0607 0.1113 0.0808
Nuclear Periphery 0.0359 0.0154 0.0193 0.0144 0.0482 0.0249 0.0392 0.0281 0.026 0.0169 0.0541 0.0196 0.0285 0.0341 0.0107 0.0221 0.0174 0.0156
Nucleolus 0.0003 0.0002 0.0009 0.0001 0.0022 0.0001 0.0076 0.0005 0.0015 0.0005 0.0025 0.0002 0.0003 0.0001 0.0001 0.001 0.0001 0.0002
Nucleus 0.0073 0.004 0.0065 0.0014 0.0047 0.0034 0.012 0.0071 0.0038 0.007 0.0809 0.0042 0.005 0.0046 0.003 0.0171 0.0023 0.0042
Peroxisomes 0.0095 0.0001 0.0024 0.0044 0.0323 0.0016 0.0075 0.0004 0.013 0.0016 0.0032 0.0001 0.0052 0.0001 0.0049 0.0001 0.0001 0.0001
Punctate Nuclear 0.004 0.0017 0.0034 0.0006 0.0096 0.0014 0.01 0.002 0.0055 0.004 0.0055 0.0035 0.002 0.0016 0.0015 0.0024 0.0006 0.0035
Vacuole 0.0072 0.0071 0.0053 0.0054 0.0055 0.0041 0.0078 0.0082 0.0072 0.0047 0.0171 0.0059 0.0053 0.0084 0.0063 0.0061 0.0029 0.0044
Vacuole Periphery 0.0025 0.0009 0.001 0.0012 0.0028 0.0009 0.0037 0.0009 0.002 0.0007 0.0086 0.0008 0.0012 0.001 0.0005 0.0012 0.0005 0.0006

Sequencing Data

R1 R2
G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Post-START S/G2 Metaphase Anaphase Telophase
Gene Expression 69.2466 38.562 18.0724 24.2729 36.7956 27.2295 38.477 24.9306 27.8346 59.8695
Translational Efficiency 1.3555 1.7145 1.6216 1.4436 1.7225 3.8556 1.6531 1.7187 1.5816 1.454

Hit Data

Dataset Hit
Protein Concentration
Protein Localization
Gene Expression
Translational Efficiency

Micrographs

Cell Count

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
1794 1195 525 1015 1545 2101 313 1227 3339 3296 838 2242

Protein Abundance

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
Mean 710.24 806.76 1152.04 1141.96 673.59 811.58 1099.49 1121.71 693.28 809.83 1132.41 1130.88
Standard Deviation 106.42 114.32 181.30 212.71 120.69 111.31 160.65 196.70 114.71 112.43 175.72 204.36
Intensity Change Log 2 0.183833 0.697812 0.685134 0.268863 0.706892 0.735757 0.225848 0.702239 0.709997

Localization Score (DeepLoc)

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
Actin 0.000186 0.000565 0.000674 0.007059 0.000202 0.000293 0.000893 0.004098 0.000194 0.000391 0.000756 0.005439
Bud Neck 0.004381 0.008229 0.001964 0.008535 0.006361 0.011817 0.002086 0.005905 0.005297 0.010516 0.002010 0.007096
Bud Site 0.002518 0.006031 0.004118 0.030057 0.002518 0.009690 0.004478 0.024863 0.002518 0.008363 0.004253 0.027215
Cell Periphery 0.000484 0.000395 0.000604 0.002667 0.000377 0.000421 0.000992 0.004053 0.000434 0.000412 0.000749 0.003426
Cytoplasm 0.373676* 0.306554* 0.103309* 0.097524 0.281026* 0.326951* 0.080271 0.101213* 0.330806* 0.319556* 0.094704 0.099543
Cytoplasmic Foci 0.131203 0.176405 0.009608 0.018127 0.144525 0.141561 0.018151 0.015683 0.137367 0.154194 0.012799 0.016790
Eisosomes 0.000273 0.000361 0.000068 0.000477 0.000405 0.000395 0.000318 0.000592 0.000334 0.000383 0.000161 0.000540
Endoplasmic Reticulum 0.201375* 0.225910* 0.595926* 0.583866* 0.232163* 0.194626* 0.624474* 0.672170* 0.215621* 0.205968* 0.606589* 0.632193*
Endosome 0.012759 0.021532 0.009268 0.017131 0.016046 0.023216 0.011752 0.016179 0.014280 0.022606 0.010196 0.016610
Golgi 0.006230 0.008776 0.000455 0.006115 0.007523 0.008029 0.000916 0.005349 0.006828 0.008300 0.000628 0.005696
Lipid Particles 0.078412 0.067069 0.007149 0.029869 0.098563 0.078580 0.017279 0.029947 0.087736 0.074407 0.010933 0.029912
Mitochondria 0.006135 0.007890 0.000438 0.003274 0.006863 0.011452 0.000402 0.003202 0.006472 0.010160 0.000425 0.003235
Mitotic Spindle 0.000626 0.003901 0.000366 0.010485 0.000373 0.004732 0.000150 0.005096 0.000509 0.004431 0.000285 0.007535
None 0.008928 0.003553 0.000431 0.001401 0.012256 0.003901 0.000217 0.001473 0.010468 0.003775 0.000351 0.001441
Nuclear Periphery 0.012668 0.009157 0.057858 0.006591 0.021557 0.014362 0.045580 0.003414 0.016781 0.012475 0.053272 0.004852
Nuclear Periphery Foci 0.007317 0.009211 0.086853 0.056338 0.012765 0.006547 0.077776 0.027676 0.009838 0.007513 0.083463 0.040652
Nucleolus 0.001313 0.001188 0.000176 0.000148 0.002253 0.001436 0.000188 0.000185 0.001748 0.001346 0.000180 0.000168
Nucleus 0.106742 0.067628 0.067402 0.021939 0.099598 0.081439 0.030032 0.018948 0.103437 0.076432 0.053444 0.020302
Peroxisomes 0.002534 0.006821 0.000163 0.000844 0.004389 0.005534 0.000505 0.001041 0.003392 0.006001 0.000291 0.000952
Vacuole 0.040465 0.065926 0.052818 0.095483 0.048040 0.071480 0.083196 0.056890 0.043970 0.069466 0.064165 0.074362
Vacuole Periphery 0.001777 0.002896 0.000349 0.002069 0.002197 0.003538 0.000346 0.002022 0.001971 0.003305 0.000348 0.002043

Localization Changes (T-score)

R1 R2 R1 & R2
UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14 UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14 UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14
Actin -3.21 -2.61 -5.35 -5.05 -4.89 -1.77 -2.13 -7.48 -7.30 -5.38 -3.54 -3.36 -8.17 -7.87 -7.05
Bud Neck -3.43 4.02 -3.71 -0.68 -5.61 -4.78 4.46 -0.35 4.39 -4.74 -6.54 6.17 -3.21 2.72 -7.62
Bud Site -3.64 -1.71 -8.95 -7.76 -7.95 -5.33 -1.59 -8.98 -5.75 -7.34 -6.31 -2.32 -12.66 -9.31 -10.85
Cell Periphery 1.58 -1.03 -4.01 -4.20 -3.66 -1.12 -1.67 -6.67 -6.60 -4.54 0.66 -2.03 -7.68 -7.76 -6.29
Cytoplasm 6.97 27.35 32.57 21.65 0.01 -5.39 18.50 20.54 27.99 -2.73 1.75 31.90 37.76 35.74 -1.59
Cytoplasmic Foci -6.82 31.65 28.81 27.18 -4.46 0.51 24.54 28.67 31.83 1.15 -4.09 40.37 40.73 41.72 -2.26
Eisosomes -1.84 9.13 -2.16 -1.06 -4.46 0.15 0.52 -1.94 -1.81 -1.48 -1.09 2.72 -3.04 -2.00 -4.24
Endoplasmic Reticulum -1.99 -22.16 -22.41 -19.06 2.57 3.53 -16.89 -29.02 -34.14* -1.17 1.31 -27.67 -36.94 -37.94 0.26
Endosome -5.50 3.35 -3.01 2.42 -5.53 -5.42 2.79 0.03 4.29 -2.16 -8.20 4.79 -2.11 4.96 -5.59
Golgi -2.96 10.85 0.61 2.46 -4.33 -0.64 11.42 2.82 3.34 -5.92 -2.53 15.82 2.14 3.92 -7.12
Lipid Particles 2.89 24.43 13.37 10.04 -10.26 4.51 19.77 15.88 13.20 -4.21 4.49 31.67 20.57 16.72 -10.53
Mitochondria -1.38 6.81 2.38 3.50 -2.48 -4.07 9.72 3.33 6.42 -2.86 -4.25 11.13 3.93 7.37 -3.78
Mitotic Spindle -2.32 0.71 -5.13 -3.11 -5.24 -4.07 0.88 -4.11 -0.56 -4.33 -4.62 0.92 -6.55 -2.74 -6.72
None 4.00 7.48 5.43 2.05 -1.00 4.80 7.29 6.26 3.17 -2.90 6.24 10.32 8.40 3.65 -2.35
Nuclear Periphery 2.32 -8.79 4.45 2.01 10.14 3.59 -3.86 10.33 8.99 7.17 3.41 -9.16 10.96 8.28 12.58
Nuclear Periphery Foci -1.68 -14.35 -13.07 -12.25 4.56 5.03 -10.60 -6.62 -9.82 7.62 2.90 -17.66 -14.57 -15.70 9.10
Nucleolus 0.28 8.75 8.65 3.27 0.10 2.43 6.96 6.92 6.81 -0.15 1.78 10.09 9.99 7.21 -0.13
Nucleus 9.60 4.83 23.13 11.99 6.97 4.40 12.53 18.92 19.46 1.66 9.39 9.43 29.90 22.88 7.12
Peroxisomes -5.11 12.76 8.12 7.25 -6.19 -1.86 9.51 7.36 8.56 -2.20 -5.55 14.61 9.94 11.39 -4.77
Vacuole -7.24 -4.02 -12.94 -8.73 -8.26 -7.36 -4.91 -5.69 -0.96 1.85 -10.99 -6.16 -13.61 -7.21 -4.32
Vacuole Periphery -3.87 8.10 -0.24 2.05 -3.56 -4.40 12.07 0.62 3.16 -3.53 -6.01 13.67 0.22 3.86 -4.98
Standard name
Human Ortholog
Description Putative protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to the endoplasmic reticulum; YLR050C is not an essential gene
Localization
Cell Percentages ER (71%)
Cell Cycle Regulation No
Subcompartmental Group ER-3

Ema19

Ema19


Nearest neighbours ( genes)

correlation threshold

0.6

1.0

0.80


Biological Process (Gene Ontology)

Molecular Function (Gene Ontology)

Cellular Component (Gene Ontology)


Endocytosis
Temp Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Vph1-GFP)
37℃
RT
Cell Cycle Omics

CYCLoPs (Ema19-GFP)
Gene / Allele Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Sac6-tdTomato)
Gene Images
Gene Images
Images are not yet available
Images are not yet available