Standard name | |
---|---|
Human Ortholog | |
Description | Putative protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to the endoplasmic reticulum; YLR050C is not an essential gene |
ensLOC | DeepLoc | |||||||||||||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Localization | WT1 | WT2 | WT3 | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 | WT1 | WT2 | WT3 | AF100 | AF140 | AF180 |
Cortical Patches | 5 | 6 | 1 | 0 | 2 | 0 | – | 2 | 2 | 2 | 3 | 3 | 0 | 1 | 1 | 45 | 38 | 31 | 8 | 4 | 8 | 4 | 8 | 9 |
Bud | 3 | 3 | 5 | 2 | 3 | 6 | – | 10 | 11 | 9 | 16 | 16 | 0 | 0 | 0 | 2 | 2 | 3 | 13 | 3 | 0 | 6 | 4 | 11 |
Bud Neck | 0 | 0 | 1 | 0 | 1 | 0 | – | 0 | 0 | 0 | 0 | 0 | 1 | 0 | 0 | 0 | 0 | 0 | 8 | 2 | 1 | 1 | 1 | 3 |
Bud Site | 1 | 0 | 2 | 0 | 0 | 0 | – | 2 | 0 | 1 | 0 | 2 | 0 | 0 | 0 | 0 | 0 | 1 | – | – | – | – | – | – |
Cell Periphery | 0 | 4 | 1 | 5 | 6 | 8 | – | 5 | 6 | 9 | 6 | 18 | 7 | 6 | 17 | 45 | 23 | 36 | 4 | 0 | 2 | 1 | 2 | 1 |
Cytoplasm | 263 | 87 | 96 | 54 | 121 | 117 | – | 144 | 125 | 163 | 144 | 178 | 96 | 98 | 164 | 69 | 48 | 62 | 110 | 23 | 22 | 24 | 49 | 53 |
Endoplasmic Reticulum | 91 | 122 | 49 | 42 | 38 | 65 | – | 77 | 76 | 67 | 57 | 60 | 80 | 57 | 54 | 3 | 8 | 5 | 349 | 180 | 148 | 121 | 161 | 142 |
Endosome | 9 | 3 | 1 | 0 | 8 | 0 | – | 4 | 3 | 7 | 2 | 5 | 1 | 0 | 1 | 10 | 1 | 3 | 6 | 5 | 1 | 1 | 3 | 7 |
Golgi | 1 | 0 | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 48 | 23 | 40 | 5 | 2 | 0 | 2 | 2 | 4 |
Mitochondria | 24 | 1 | 51 | 56 | 35 | 126 | – | 161 | 194 | 186 | 175 | 236 | 2 | 3 | 2 | 15 | 13 | 12 | 34 | 13 | 27 | 23 | 33 | 17 |
Nucleus | 2 | 1 | 1 | 0 | 0 | 0 | – | 6 | 1 | 2 | 1 | 3 | 0 | 1 | 0 | 2 | 4 | 5 | 1 | 0 | 0 | 0 | 0 | 0 |
Nuclear Periphery | 1 | 1 | 1 | 1 | 0 | 3 | – | 10 | 19 | 11 | 2 | 8 | 1 | 0 | 0 | 0 | 1 | 2 | 3 | 3 | 4 | 2 | 2 | 0 |
Nucleolus | 0 | 0 | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 1 | 0 | 0 | 0 | 0 | 0 | 1 | 1 | 0 | 0 | 0 | 0 | 0 | 0 |
Peroxisomes | 0 | 0 | 0 | 0 | 0 | 0 | – | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 2 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
SpindlePole | 0 | 0 | 0 | 0 | 0 | 0 | – | 1 | 1 | 1 | 1 | 1 | 0 | 0 | 0 | 2 | 1 | 2 | 4 | 0 | 1 | 0 | 2 | 1 |
Vac/Vac Membrane | 79 | 20 | 13 | 0 | 20 | 14 | – | 29 | 26 | 43 | 17 | 27 | 2 | 1 | 5 | 75 | 56 | 88 | 19 | 8 | 4 | 7 | 17 | 21 |
Unique Cell Count | 435 | 216 | 192 | 123 | 196 | 265 | 369 | 350 | 398 | 337 | 444 | 177 | 154 | 227 | 258 | 179 | 234 | 576 | 256 | 229 | 202 | 295 | 281 | |
Labelled Cell Count | 479 | 248 | 222 | 160 | 234 | 339 | 452 | 464 | 501 | 425 | 557 | 190 | 167 | 245 | 318 | 219 | 291 | 576 | 256 | 229 | 202 | 295 | 281 |
Screen | WT1 | WT2 | WT3 | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 | AF100 | AF140 | AF180 |
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Mean Cell GFP Intensity (1e-4) | 5.6 | 7.2 | 4.5 | 4.9 | 5.8 | 4.7 | – | 5.0 | 4.6 | 4.9 | 4.8 | 4.8 | 6.4 | 6.8 | 6.4 | 11.6 | 11.1 | 11.0 | 6.5 | 6.6 | 7.0 |
Std Deviation (1e-4) | 1.2 | 1.6 | 1.1 | 1.1 | 2.1 | 1.3 | – | 1.7 | 1.5 | 1.4 | 1.7 | 1.6 | 1.4 | 1.6 | 1.5 | 2.7 | 2.5 | 2.4 | 1.7 | 1.8 | 1.9 |
Intensity Change (Log2) | – | – | – | 0.11 | 0.37 | 0.07 | – | 0.15 | 0.02 | 0.12 | 0.11 | 0.08 | 0.51 | 0.61 | 0.52 | 1.37 | 1.3 | 1.3 | 0.54 | 0.55 | 0.65 |
Localization | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 |
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Cortical Patches | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 5.9 | 6.5 | 5.0 |
Bud | 0 | 0 | 0 | – | 0.1 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Bud Neck | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Bud Site | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Cell Periphery | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 3.5 | 5.9 | 4.8 | 5.4 |
Cytoplasm | -1.1 | 2.3 | -1.2 | – | -2.5 | -3.2 | -2.1 | -1.6 | -2.3 | 0.8 | 2.5 | 4.7 | -5.1 | -4.6 | -5.0 |
Endoplasmic Reticulum | 0 | -1.4 | 0 | – | -1.3 | 0 | -2.5 | 0 | 0 | 4.0 | 2.3 | -0.4 | -8.0 | -5.6 | -7.2 |
Endosome | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Golgi | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 6.3 | 5.1 | 6.0 |
Mitochondria | 0 | -2.1 | 0 | – | 4.0 | 0 | 4.7 | 0 | 0 | -7.0 | -6.3 | -7.9 | -6.2 | -4.9 | -6.2 |
Nucleus | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Nuclear Periphery | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Nucleolus | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Peroxisomes | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
SpindlePole | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Vacuole | -2.9 | 1.2 | -0.7 | – | 0.5 | 0.3 | 1.6 | -0.8 | -0.3 | -2.7 | -2.9 | -2.3 | 5.9 | 6.1 | 7.4 |
R1 | R2 | R3 | ||||||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | |
Concentration | 2.2053 | 2.6698 | 2.2548 | 2.4825 | 2.0476 | 2.5568 | 2.5992 | 2.235 | 2.6879 | 1.7503 | 2.3712 | 1.7933 | 3.5466 | 3.5868 | 3.4065 | 3.353 | 3.0762 | 3.3758 |
Actin | 0.0091 | 0.0024 | 0.0138 | 0.0018 | 0.011 | 0.0009 | 0.0338 | 0.0035 | 0.0101 | 0.0024 | 0.0155 | 0.0013 | 0.0272 | 0.0005 | 0.0098 | 0.0011 | 0.0009 | 0.0015 |
Bud | 0.0002 | 0.0002 | 0.0008 | 0.0001 | 0.0003 | 0 | 0.0014 | 0.0022 | 0.0012 | 0.0003 | 0.0363 | 0.0001 | 0.0002 | 0.0001 | 0.0001 | 0.0014 | 0 | 0 |
Bud Neck | 0.0002 | 0.0008 | 0.0015 | 0.0003 | 0.0006 | 0.0005 | 0.003 | 0.0003 | 0.0009 | 0.0167 | 0.0028 | 0.0076 | 0.0011 | 0.0002 | 0.0008 | 0.0006 | 0.0005 | 0.0018 |
Bud Periphery | 0.0003 | 0.0011 | 0.0014 | 0.0002 | 0.0008 | 0.0001 | 0.0012 | 0.0006 | 0.0013 | 0.0004 | 0.0189 | 0.0002 | 0.0004 | 0.0001 | 0.0003 | 0.0007 | 0 | 0.0001 |
Bud Site | 0.0006 | 0.0044 | 0.0109 | 0.0001 | 0.0011 | 0.0001 | 0.0079 | 0.009 | 0.0152 | 0.0051 | 0.0045 | 0.0001 | 0.0009 | 0.0105 | 0.0008 | 0.0003 | 0 | 0.0001 |
Cell Periphery | 0.0008 | 0.0024 | 0.0014 | 0.0008 | 0.0003 | 0.0004 | 0.0012 | 0.0023 | 0.002 | 0.0004 | 0.0012 | 0.0006 | 0.001 | 0.0027 | 0.0019 | 0.0018 | 0.0003 | 0.0004 |
Cytoplasm | 0.1307 | 0.2514 | 0.1685 | 0.1097 | 0.0965 | 0.1912 | 0.1434 | 0.2406 | 0.1867 | 0.1742 | 0.1835 | 0.2486 | 0.14 | 0.2631 | 0.2546 | 0.2175 | 0.2016 | 0.2488 |
Cytoplasmic Foci | 0.0128 | 0.006 | 0.0156 | 0.0169 | 0.0137 | 0.0056 | 0.0225 | 0.01 | 0.0303 | 0.0111 | 0.0205 | 0.0067 | 0.0056 | 0.0052 | 0.0136 | 0.0055 | 0.0031 | 0.0083 |
Eisosomes | 0.0002 | 0.0002 | 0.0002 | 0.0001 | 0.001 | 0 | 0.0009 | 0.0001 | 0.0005 | 0.0001 | 0.0005 | 0 | 0.0006 | 0.0001 | 0.0002 | 0.0001 | 0.0001 | 0 |
Endoplasmic Reticulum | 0.6161 | 0.5797 | 0.6313 | 0.6916 | 0.5517 | 0.6614 | 0.5166 | 0.5562 | 0.5768 | 0.6566 | 0.3115 | 0.6391 | 0.661 | 0.5624 | 0.5636 | 0.6368 | 0.6387 | 0.6083 |
Endosome | 0.0348 | 0.0167 | 0.0218 | 0.0262 | 0.0274 | 0.0167 | 0.0483 | 0.0251 | 0.0223 | 0.0213 | 0.0659 | 0.0108 | 0.0195 | 0.0152 | 0.0214 | 0.0126 | 0.0114 | 0.0155 |
Golgi | 0.0041 | 0.0012 | 0.0049 | 0.0078 | 0.0084 | 0.0014 | 0.0096 | 0.0031 | 0.0052 | 0.0049 | 0.0218 | 0.0005 | 0.0065 | 0.0008 | 0.0034 | 0.0012 | 0.0016 | 0.0007 |
Lipid Particles | 0.0218 | 0.0058 | 0.016 | 0.0632 | 0.0192 | 0.0063 | 0.032 | 0.0104 | 0.0168 | 0.0074 | 0.0044 | 0.0032 | 0.0147 | 0.0074 | 0.0086 | 0.0084 | 0.0065 | 0.0044 |
Mitochondria | 0.0085 | 0.0098 | 0.0058 | 0.0343 | 0.0436 | 0.0019 | 0.0123 | 0.008 | 0.0162 | 0.0011 | 0.0189 | 0.0005 | 0.0028 | 0.0003 | 0.0011 | 0.001 | 0.0003 | 0.0006 |
None | 0.093 | 0.0884 | 0.0674 | 0.0195 | 0.1188 | 0.0771 | 0.078 | 0.0816 | 0.0555 | 0.0628 | 0.1218 | 0.0466 | 0.0708 | 0.0813 | 0.0928 | 0.0607 | 0.1113 | 0.0808 |
Nuclear Periphery | 0.0359 | 0.0154 | 0.0193 | 0.0144 | 0.0482 | 0.0249 | 0.0392 | 0.0281 | 0.026 | 0.0169 | 0.0541 | 0.0196 | 0.0285 | 0.0341 | 0.0107 | 0.0221 | 0.0174 | 0.0156 |
Nucleolus | 0.0003 | 0.0002 | 0.0009 | 0.0001 | 0.0022 | 0.0001 | 0.0076 | 0.0005 | 0.0015 | 0.0005 | 0.0025 | 0.0002 | 0.0003 | 0.0001 | 0.0001 | 0.001 | 0.0001 | 0.0002 |
Nucleus | 0.0073 | 0.004 | 0.0065 | 0.0014 | 0.0047 | 0.0034 | 0.012 | 0.0071 | 0.0038 | 0.007 | 0.0809 | 0.0042 | 0.005 | 0.0046 | 0.003 | 0.0171 | 0.0023 | 0.0042 |
Peroxisomes | 0.0095 | 0.0001 | 0.0024 | 0.0044 | 0.0323 | 0.0016 | 0.0075 | 0.0004 | 0.013 | 0.0016 | 0.0032 | 0.0001 | 0.0052 | 0.0001 | 0.0049 | 0.0001 | 0.0001 | 0.0001 |
Punctate Nuclear | 0.004 | 0.0017 | 0.0034 | 0.0006 | 0.0096 | 0.0014 | 0.01 | 0.002 | 0.0055 | 0.004 | 0.0055 | 0.0035 | 0.002 | 0.0016 | 0.0015 | 0.0024 | 0.0006 | 0.0035 |
Vacuole | 0.0072 | 0.0071 | 0.0053 | 0.0054 | 0.0055 | 0.0041 | 0.0078 | 0.0082 | 0.0072 | 0.0047 | 0.0171 | 0.0059 | 0.0053 | 0.0084 | 0.0063 | 0.0061 | 0.0029 | 0.0044 |
Vacuole Periphery | 0.0025 | 0.0009 | 0.001 | 0.0012 | 0.0028 | 0.0009 | 0.0037 | 0.0009 | 0.002 | 0.0007 | 0.0086 | 0.0008 | 0.0012 | 0.001 | 0.0005 | 0.0012 | 0.0005 | 0.0006 |
R1 | R2 | |||||||||
---|---|---|---|---|---|---|---|---|---|---|
G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | |
Gene Expression | 69.2466 | 38.562 | 18.0724 | 24.2729 | 36.7956 | 27.2295 | 38.477 | 24.9306 | 27.8346 | 59.8695 |
Translational Efficiency | 1.3555 | 1.7145 | 1.6216 | 1.4436 | 1.7225 | 3.8556 | 1.6531 | 1.7187 | 1.5816 | 1.454 |
Dataset | Hit |
---|---|
Protein Concentration | ✘ |
Protein Localization | ✘ |
Gene Expression | ✘ |
Translational Efficiency | ✘ |
R1 | R2 | R1 & R2 | |||||||||
---|---|---|---|---|---|---|---|---|---|---|---|
WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 |
1794 | 1195 | 525 | 1015 | 1545 | 2101 | 313 | 1227 | 3339 | 3296 | 838 | 2242 |
R1 | R2 | R1 & R2 | ||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|
WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 | |
Mean | 710.24 | 806.76 | 1152.04 | 1141.96 | 673.59 | 811.58 | 1099.49 | 1121.71 | 693.28 | 809.83 | 1132.41 | 1130.88 |
Standard Deviation | 106.42 | 114.32 | 181.30 | 212.71 | 120.69 | 111.31 | 160.65 | 196.70 | 114.71 | 112.43 | 175.72 | 204.36 |
Intensity Change Log 2 | — | 0.183833 | 0.697812 | 0.685134 | — | 0.268863 | 0.706892 | 0.735757 | — | 0.225848 | 0.702239 | 0.709997 |
R1 | R2 | R1 & R2 | ||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|
WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 | |
Actin | 0.000186 | 0.000565 | 0.000674 | 0.007059 | 0.000202 | 0.000293 | 0.000893 | 0.004098 | 0.000194 | 0.000391 | 0.000756 | 0.005439 |
Bud Neck | 0.004381 | 0.008229 | 0.001964 | 0.008535 | 0.006361 | 0.011817 | 0.002086 | 0.005905 | 0.005297 | 0.010516 | 0.002010 | 0.007096 |
Bud Site | 0.002518 | 0.006031 | 0.004118 | 0.030057 | 0.002518 | 0.009690 | 0.004478 | 0.024863 | 0.002518 | 0.008363 | 0.004253 | 0.027215 |
Cell Periphery | 0.000484 | 0.000395 | 0.000604 | 0.002667 | 0.000377 | 0.000421 | 0.000992 | 0.004053 | 0.000434 | 0.000412 | 0.000749 | 0.003426 |
Cytoplasm | 0.373676* | 0.306554* | 0.103309* | 0.097524 | 0.281026* | 0.326951* | 0.080271 | 0.101213* | 0.330806* | 0.319556* | 0.094704 | 0.099543 |
Cytoplasmic Foci | 0.131203 | 0.176405 | 0.009608 | 0.018127 | 0.144525 | 0.141561 | 0.018151 | 0.015683 | 0.137367 | 0.154194 | 0.012799 | 0.016790 |
Eisosomes | 0.000273 | 0.000361 | 0.000068 | 0.000477 | 0.000405 | 0.000395 | 0.000318 | 0.000592 | 0.000334 | 0.000383 | 0.000161 | 0.000540 |
Endoplasmic Reticulum | 0.201375* | 0.225910* | 0.595926* | 0.583866* | 0.232163* | 0.194626* | 0.624474* | 0.672170* | 0.215621* | 0.205968* | 0.606589* | 0.632193* |
Endosome | 0.012759 | 0.021532 | 0.009268 | 0.017131 | 0.016046 | 0.023216 | 0.011752 | 0.016179 | 0.014280 | 0.022606 | 0.010196 | 0.016610 |
Golgi | 0.006230 | 0.008776 | 0.000455 | 0.006115 | 0.007523 | 0.008029 | 0.000916 | 0.005349 | 0.006828 | 0.008300 | 0.000628 | 0.005696 |
Lipid Particles | 0.078412 | 0.067069 | 0.007149 | 0.029869 | 0.098563 | 0.078580 | 0.017279 | 0.029947 | 0.087736 | 0.074407 | 0.010933 | 0.029912 |
Mitochondria | 0.006135 | 0.007890 | 0.000438 | 0.003274 | 0.006863 | 0.011452 | 0.000402 | 0.003202 | 0.006472 | 0.010160 | 0.000425 | 0.003235 |
Mitotic Spindle | 0.000626 | 0.003901 | 0.000366 | 0.010485 | 0.000373 | 0.004732 | 0.000150 | 0.005096 | 0.000509 | 0.004431 | 0.000285 | 0.007535 |
None | 0.008928 | 0.003553 | 0.000431 | 0.001401 | 0.012256 | 0.003901 | 0.000217 | 0.001473 | 0.010468 | 0.003775 | 0.000351 | 0.001441 |
Nuclear Periphery | 0.012668 | 0.009157 | 0.057858 | 0.006591 | 0.021557 | 0.014362 | 0.045580 | 0.003414 | 0.016781 | 0.012475 | 0.053272 | 0.004852 |
Nuclear Periphery Foci | 0.007317 | 0.009211 | 0.086853 | 0.056338 | 0.012765 | 0.006547 | 0.077776 | 0.027676 | 0.009838 | 0.007513 | 0.083463 | 0.040652 |
Nucleolus | 0.001313 | 0.001188 | 0.000176 | 0.000148 | 0.002253 | 0.001436 | 0.000188 | 0.000185 | 0.001748 | 0.001346 | 0.000180 | 0.000168 |
Nucleus | 0.106742 | 0.067628 | 0.067402 | 0.021939 | 0.099598 | 0.081439 | 0.030032 | 0.018948 | 0.103437 | 0.076432 | 0.053444 | 0.020302 |
Peroxisomes | 0.002534 | 0.006821 | 0.000163 | 0.000844 | 0.004389 | 0.005534 | 0.000505 | 0.001041 | 0.003392 | 0.006001 | 0.000291 | 0.000952 |
Vacuole | 0.040465 | 0.065926 | 0.052818 | 0.095483 | 0.048040 | 0.071480 | 0.083196 | 0.056890 | 0.043970 | 0.069466 | 0.064165 | 0.074362 |
Vacuole Periphery | 0.001777 | 0.002896 | 0.000349 | 0.002069 | 0.002197 | 0.003538 | 0.000346 | 0.002022 | 0.001971 | 0.003305 | 0.000348 | 0.002043 |
R1 | R2 | R1 & R2 | |||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
UBP2_WT | UBP14_WT | UBP2UBP14_WT | UBP2UBP14_UBP2 | UBP2UBP14_UBP14 | UBP2_WT | UBP14_WT | UBP2UBP14_WT | UBP2UBP14_UBP2 | UBP2UBP14_UBP14 | UBP2_WT | UBP14_WT | UBP2UBP14_WT | UBP2UBP14_UBP2 | UBP2UBP14_UBP14 | |
Actin | -3.21 | -2.61 | -5.35 | -5.05 | -4.89 | -1.77 | -2.13 | -7.48 | -7.30 | -5.38 | -3.54 | -3.36 | -8.17 | -7.87 | -7.05 |
Bud Neck | -3.43 | 4.02 | -3.71 | -0.68 | -5.61 | -4.78 | 4.46 | -0.35 | 4.39 | -4.74 | -6.54 | 6.17 | -3.21 | 2.72 | -7.62 |
Bud Site | -3.64 | -1.71 | -8.95 | -7.76 | -7.95 | -5.33 | -1.59 | -8.98 | -5.75 | -7.34 | -6.31 | -2.32 | -12.66 | -9.31 | -10.85 |
Cell Periphery | 1.58 | -1.03 | -4.01 | -4.20 | -3.66 | -1.12 | -1.67 | -6.67 | -6.60 | -4.54 | 0.66 | -2.03 | -7.68 | -7.76 | -6.29 |
Cytoplasm | 6.97 | 27.35 | 32.57 | 21.65 | 0.01 | -5.39 | 18.50 | 20.54 | 27.99 | -2.73 | 1.75 | 31.90 | 37.76 | 35.74 | -1.59 |
Cytoplasmic Foci | -6.82 | 31.65 | 28.81 | 27.18 | -4.46 | 0.51 | 24.54 | 28.67 | 31.83 | 1.15 | -4.09 | 40.37 | 40.73 | 41.72 | -2.26 |
Eisosomes | -1.84 | 9.13 | -2.16 | -1.06 | -4.46 | 0.15 | 0.52 | -1.94 | -1.81 | -1.48 | -1.09 | 2.72 | -3.04 | -2.00 | -4.24 |
Endoplasmic Reticulum | -1.99 | -22.16 | -22.41 | -19.06 | 2.57 | 3.53 | -16.89 | -29.02 | -34.14* | -1.17 | 1.31 | -27.67 | -36.94 | -37.94 | 0.26 |
Endosome | -5.50 | 3.35 | -3.01 | 2.42 | -5.53 | -5.42 | 2.79 | 0.03 | 4.29 | -2.16 | -8.20 | 4.79 | -2.11 | 4.96 | -5.59 |
Golgi | -2.96 | 10.85 | 0.61 | 2.46 | -4.33 | -0.64 | 11.42 | 2.82 | 3.34 | -5.92 | -2.53 | 15.82 | 2.14 | 3.92 | -7.12 |
Lipid Particles | 2.89 | 24.43 | 13.37 | 10.04 | -10.26 | 4.51 | 19.77 | 15.88 | 13.20 | -4.21 | 4.49 | 31.67 | 20.57 | 16.72 | -10.53 |
Mitochondria | -1.38 | 6.81 | 2.38 | 3.50 | -2.48 | -4.07 | 9.72 | 3.33 | 6.42 | -2.86 | -4.25 | 11.13 | 3.93 | 7.37 | -3.78 |
Mitotic Spindle | -2.32 | 0.71 | -5.13 | -3.11 | -5.24 | -4.07 | 0.88 | -4.11 | -0.56 | -4.33 | -4.62 | 0.92 | -6.55 | -2.74 | -6.72 |
None | 4.00 | 7.48 | 5.43 | 2.05 | -1.00 | 4.80 | 7.29 | 6.26 | 3.17 | -2.90 | 6.24 | 10.32 | 8.40 | 3.65 | -2.35 |
Nuclear Periphery | 2.32 | -8.79 | 4.45 | 2.01 | 10.14 | 3.59 | -3.86 | 10.33 | 8.99 | 7.17 | 3.41 | -9.16 | 10.96 | 8.28 | 12.58 |
Nuclear Periphery Foci | -1.68 | -14.35 | -13.07 | -12.25 | 4.56 | 5.03 | -10.60 | -6.62 | -9.82 | 7.62 | 2.90 | -17.66 | -14.57 | -15.70 | 9.10 |
Nucleolus | 0.28 | 8.75 | 8.65 | 3.27 | 0.10 | 2.43 | 6.96 | 6.92 | 6.81 | -0.15 | 1.78 | 10.09 | 9.99 | 7.21 | -0.13 |
Nucleus | 9.60 | 4.83 | 23.13 | 11.99 | 6.97 | 4.40 | 12.53 | 18.92 | 19.46 | 1.66 | 9.39 | 9.43 | 29.90 | 22.88 | 7.12 |
Peroxisomes | -5.11 | 12.76 | 8.12 | 7.25 | -6.19 | -1.86 | 9.51 | 7.36 | 8.56 | -2.20 | -5.55 | 14.61 | 9.94 | 11.39 | -4.77 |
Vacuole | -7.24 | -4.02 | -12.94 | -8.73 | -8.26 | -7.36 | -4.91 | -5.69 | -0.96 | 1.85 | -10.99 | -6.16 | -13.61 | -7.21 | -4.32 |
Vacuole Periphery | -3.87 | 8.10 | -0.24 | 2.05 | -3.56 | -4.40 | 12.07 | 0.62 | 3.16 | -3.53 | -6.01 | 13.67 | 0.22 | 3.86 | -4.98 |
Temp | Actin Patch (Sac6-tdTomato) | Cortical Patch (Sla1-GFP) | Late Endosome (Snf7-GFP) | Vacuole (Vph1-GFP) |
---|---|---|---|---|
37℃ | ||||
RT |
Gene / Allele | Actin Patch (Sac6-tdTomato) | Cortical Patch (Sla1-GFP) | Late Endosome (Snf7-GFP) | Vacuole (Sac6-tdTomato) |
---|
Gene | Images |
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Gene | Images |
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