Standard name
Human Ortholog
Description Subunit of the SAGA transcriptional regulatory complex; not present in SAGA-like complex SLIK/SALSA; required for SAGA-mediated inhibition at some promoters
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Micrographs

ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 0 – 0 0 0 0 – – – 0 0 0 0 0 0 0 0 0
Bud 0 0 0 0 0 0 0 – 0 0 0 0 – – – 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 – 0 0 0 0 – – – 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 – 0 0 0 0 – – – 0 0 0 – – – – – –
Cell Periphery 0 0 0 0 0 0 0 – 0 0 0 0 – – – 0 0 0 0 0 0 0 0 0
Cytoplasm 0.05 0.05 0.05 0.05 0.14 0.09 0 – 0.06 0 0 0 – – – 0.11 0.06 0.1 0 0 0 0.06 0.07 0
Endoplasmic Reticulum 0 0 0 0 0 0 0 – 0 0 0 0 – – – 0 0 0.05 0 0 0 0 0 0
Endosome 0 0 0 0 0 0 0 – 0 0 0 0 – – – 0.07 0 0 0 0 0 0.14 0.06 0
Golgi 0 0 0 0 0 0 0 – 0 0 0 0 – – – 0 0 0 0 0 0 0 0 0.06
Mitochondria 0.15 0 0 0 0 0.09 0 – 0.21 0.17 0.34 0.18 – – – 0 0.07 0.07 0 0 0 0 0 0
Nucleus 0.79 0.84 0.8 0.95 0.83 0.87 0.91 – 0.72 0.78 0.72 0.78 – – – 0.75 0.77 0.76 0.69 0.73 0.56 0.29 0.35 0.56
Nuclear Periphery 0 0 0 0 0 0 0 – 0 0 0 0 – – – 0 0 0 0 0 0 0.14 0 0
Nucleolus 0.08 0 0 0 0 0.05 0.06 – 0.28 0.22 0.34 0.37 – – – 0 0 0 0.12 0.12 0.14 0 0.17 0
Peroxisomes 0 0 0 0 0 0 0 – 0 0 0 0 – – – 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0.05 0 0 0 0 – 0 0 0 0 – – – 0 0 0 0.05 0 0 0 0.11 0
Vac/Vac Membrane 0 0.1 0.11 0 0 0 0 – 0 0 0 0 – – – 0.08 0.07 0.1 0.07 0.05 0.14 0 0.06 0.14
ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 0 – 0 0 0 0 – – – 0 0 0 1 0 0 0 0 0
Bud 0 0 0 0 0 0 0 – 0 0 0 0 – – – 0 0 0 0 5 1 0 0 0
Bud Neck 0 0 1 0 0 0 0 – 2 0 0 0 – – – 0 0 0 0 0 0 0 0 0
Bud Site 1 0 0 0 0 0 3 – 2 5 4 4 – – – 0 1 0 – – – – – –
Cell Periphery 0 0 0 0 0 0 0 – 0 0 0 0 – – – 2 0 0 0 0 0 0 0 0
Cytoplasm 7 7 2 1 13 11 6 – 7 2 6 1 – – – 27 13 24 1 1 1 0 0 0
Endoplasmic Reticulum 0 1 0 0 0 0 0 – 0 0 0 1 – – – 9 9 12 0 0 0 0 0 0
Endosome 6 3 1 0 1 1 0 – 0 0 0 0 – – – 16 9 4 2 3 3 1 0 0
Golgi 0 0 0 0 0 0 0 – 0 0 0 0 – – – 0 1 1 0 0 0 0 0 1
Mitochondria 22 1 0 0 1 11 6 – 25 27 52 26 – – – 7 14 17 2 1 0 0 0 0
Nucleus 112 130 35 18 77 111 153 – 86 123 110 114 – – – 176 157 186 114 132 54 2 4 17
Nuclear Periphery 0 0 0 0 0 0 1 – 0 2 0 0 – – – 0 0 1 1 0 1 1 0 0
Nucleolus 12 2 1 0 2 6 10 – 33 34 51 54 – – – 6 3 4 19 22 13 0 2 0
Peroxisomes 0 0 0 0 0 0 0 – 0 0 0 0 – – – 0 0 0 0 0 0 0 0 0
SpindlePole 1 1 2 0 1 0 1 – 1 0 0 0 – – – 5 3 5 8 4 4 0 1 0
Vac/Vac Membrane 6 15 5 0 1 3 1 – 0 0 0 2 – – – 19 15 24 11 9 14 0 0 4
Unique Cell Count 142 155 44 19 93 127 169 120 157 152 147 236 204 245 165 183 97 9 13 31
Labelled Cell Count 167 160 47 19 96 143 181 156 193 223 202 267 225 278 165 183 97 9 13 31


Nucleus

(Huh et al. Nature 2003 425:686)
Screen WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 AF100 AF140 AF180
Mean Cell GFP Intensity (1e-4) 5.4 7.0 6.4 5.9 7.9 6.5 6.6 – 5.5 5.7 5.5 5.6 – – – 7.7 7.8 7.5 – – –
Std Deviation (1e-4) 1.2 1.0 1.1 0.8 2.2 1.7 1.4 – 1.6 1.2 1.5 1.4 – – – 1.8 1.3 1.6 – – –
Intensity Change (Log2) – – – -0.1 0.32 0.02 0.04 – -0.21 -0.15 -0.2 -0.2 – – – 0.28 0.28 0.23 – – –


Localization RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3
Cortical Patches 0 0 0 0 – 0 0 0 0 – – – 0 0 0
Bud 0 0 0 0 – 0 0 0 0 – – – 0 0 0
Bud Neck 0 0 0 0 – 0 0 0 0 – – – 0 0 0
Bud Site 0 0 0 0 – 0 0 0 0 – – – 0 0 0
Cell Periphery 0 0 0 0 – 0 0 0 0 – – – 0 0 0
Cytoplasm 0 0 0 0 – 0 0 0 0 – – – 0 0 0
Endoplasmic Reticulum 0 0 0 0 – 0 0 0 0 – – – 0 0 0
Endosome 0 0 0 0 – 0 0 0 0 – – – 0 0 0
Golgi 0 0 0 0 – 0 0 0 0 – – – 0 0 0
Mitochondria 0 0 0 0 – 3.3 3.0 4.5 3.0 – – – 0 0 0
Nucleus 1.5 0.5 1.3 2.0 – -1.0 -0.2 -1.0 -0.3 – – – -0.7 -0.4 -0.5
Nuclear Periphery 0 0 0 0 – 0 0 0 0 – – – 0 0 0
Nucleolus 0 0 0 0 – 3.5 3.0 4.1 4.4 – – – 0 0 0
Peroxisomes 0 0 0 0 – 0 0 0 0 – – – 0 0 0
SpindlePole 0 0 0 0 – 0 0 0 0 – – – 0 0 0
Vacuole 0 0 0 0 – 0 0 0 0 – – – 0 0 0

Images

Circle plot not available

Protein Concentration and Protein Localization Data

R1 R2 R3
G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase
Concentration 2.756 2.7007 2.4569 2.1489 1.804 2.3397 2.0943 2.049 2.0359 1.9603 1.9401 1.898 2.1192 2.8495 2.0057 2.3086 2.242 1.861
Actin 0.0047 0.0007 0.0012 0 0.0019 0.002 0.0033 0.0001 0.0076 0.0057 0.0023 0.0023 0.0668 0.0125 0.0838 0 0.002 0.0007
Bud 0.0008 0.0001 0.0006 0 0.0003 0.0001 0.0003 0 0.0004 0.0021 0.0005 0.0001 0.0009 0.0006 0.0004 0.0001 0.0002 0.0001
Bud Neck 0.0024 0.0002 0.0009 0.0001 0.0019 0.0014 0.0078 0.0001 0.0003 0.0016 0.0013 0.0012 0.0041 0.0005 0.0004 0.0002 0.0002 0.0008
Bud Periphery 0.0021 0.0002 0.0008 0 0.0012 0.0002 0.0006 0 0.0009 0.0035 0.002 0.0004 0.0011 0.001 0.0006 0.0001 0.0002 0.0001
Bud Site 0.0028 0.0003 0.0005 0 0.0005 0.0002 0.0055 0.0001 0.0025 0.0033 0.0007 0.0003 0.0128 0.0022 0.0017 0.0001 0.0007 0.0001
Cell Periphery 0.0005 0 0.0001 0 0.0003 0 0.0003 0 0.0001 0.0004 0.0004 0.0001 0.0003 0.0002 0.0001 0 0 0
Cytoplasm 0.0118 0.0017 0.0046 0.0004 0.0059 0.0002 0.0084 0.0001 0.0008 0.0032 0.0026 0.0002 0.0369 0.0035 0.0027 0.0005 0.0022 0.0001
Cytoplasmic Foci 0.0083 0.0004 0.0128 0.0072 0.0015 0.0022 0.0093 0.0008 0.0093 0.0103 0.0113 0.0021 0.0602 0.0003 0.0028 0 0.0339 0.0055
Eisosomes 0.0001 0 0 0 0.0001 0 0.0002 0 0 0.0001 0.0001 0 0.0007 0 0.0003 0 0 0
Endoplasmic Reticulum 0.0066 0.0003 0.0022 0.0001 0.0021 0.0004 0.0016 0 0.0005 0.0024 0.002 0.0003 0.0078 0.0005 0.0113 0 0.0016 0.0001
Endosome 0.0205 0.0009 0.0187 0.0308 0.0204 0.0087 0.0118 0.0018 0.0089 0.0526 0.0328 0.0015 0.053 0.0009 0.0182 0 0.0689 0.025
Golgi 0.0056 0.0004 0.0047 0.0008 0.0015 0.0062 0.0045 0.0009 0.0022 0.0221 0.0099 0.0009 0.0353 0.0002 0.0093 0 0.0101 0.0192
Lipid Particles 0.0175 0.0015 0.0044 0.0014 0.0009 0.0116 0.0227 0.0037 0.0047 0.0233 0.0136 0.0033 0.0225 0.0001 0.009 0 0.043 0.0079
Mitochondria 0.023 0.0036 0.0183 0.0001 0.0383 0.0037 0.0018 0.0001 0.0026 0.0298 0.0085 0.0048 0.0113 0.0015 0.008 0.0003 0.0018 0.0048
None 0.0021 0.0001 0.0011 0.0001 0.0012 0.0002 0.0105 0 0.0004 0.0006 0.0044 0.0003 0.0269 0.0001 0.0007 0.0002 0.0026 0
Nuclear Periphery 0.0174 0.0056 0.0099 0.0034 0.0184 0.0039 0.0039 0.0005 0.0043 0.006 0.0218 0.0035 0.0176 0.0018 0.0194 0.0015 0.0065 0.0004
Nucleolus 0.0338 0.0272 0.0153 0.0171 0.1248 0.0755 0.0218 0.0263 0.0143 0.039 0.1168 0.0596 0.0197 0.0329 0.0219 0.0399 0.0282 0.0482
Nucleus 0.8194 0.9501 0.877 0.9308 0.7186 0.8646 0.8649 0.9643 0.9238 0.7633 0.7389 0.9059 0.5604 0.9389 0.8011 0.9557 0.7725 0.8833
Peroxisomes 0.0049 0.0004 0.0027 0.0001 0.0003 0.0025 0.0088 0.0003 0.0095 0.008 0.0059 0.0071 0.0218 0.0001 0.0037 0 0.0068 0.0019
Punctate Nuclear 0.0062 0.0054 0.0079 0.0065 0.0508 0.0144 0.006 0.0008 0.0031 0.0046 0.0157 0.0053 0.036 0.0004 0.0029 0.0012 0.0156 0.0006
Vacuole 0.0063 0.0006 0.008 0.0009 0.0057 0.0009 0.0046 0.0001 0.0026 0.0142 0.0055 0.0005 0.0022 0.0016 0.0008 0.0001 0.0017 0.0005
Vacuole Periphery 0.0034 0.0003 0.0082 0.0001 0.0036 0.0008 0.0012 0 0.001 0.0038 0.0031 0.0003 0.0015 0.0004 0.001 0 0.0012 0.0006

Sequencing Data

R1 R2
G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Post-START S/G2 Metaphase Anaphase Telophase
Gene Expression 16.4687 22.9009 27.5133 24.4677 20.957 23.1654 23.7064 30.326 35.5445 21.6296
Translational Efficiency 1.3229 1.017 0.9076 1.2702 0.9608 0.7623 0.8445 0.7194 0.5827 0.7714

Hit Data

Dataset Hit
Protein Concentration ✘
Protein Localization ✘
Gene Expression ✘
Translational Efficiency ✘

Micrographs

Cell Count

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
1554 1300 393 1298 2032 1893 216 236 3586 3193 609 1534

Protein Abundance

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
Mean 685.50 809.03 976.56 813.31 812.47 833.64 951.96 894.85 757.45 823.62 967.83 825.85
Standard Deviation 80.86 132.01 102.74 110.29 100.81 120.63 98.55 116.95 112.03 125.97 101.95 115.16
Intensity Change Log 2 — 0.239037 0.510552 0.246649 — 0.037110 0.228586 0.139331 — 0.133034 0.364485 0.189434

Localization Score (DeepLoc)

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
Actin 0.000100 0.000675 0.001193 0.001012 0.000177 0.000552 0.000751 0.000569 0.000144 0.000602 0.001036 0.000943
Bud Neck 0.003208 0.010055 0.012663 0.018884 0.004763 0.016423 0.009596 0.012090 0.004089 0.013831 0.011576 0.017839
Bud Site 0.001685 0.002575 0.002193 0.025537 0.000439 0.004643 0.004689 0.003246 0.000979 0.003801 0.003079 0.022108
Cell Periphery 0.000188 0.000113 0.000079 0.000192 0.000054 0.000097 0.000106 0.000117 0.000112 0.000103 0.000089 0.000180
Cytoplasm 0.135377* 0.031842 0.006290 0.159157* 0.005439 0.016220 0.024322 0.115395* 0.061748 0.022580 0.012685 0.152424*
Cytoplasmic Foci 0.010914 0.003986 0.000263 0.002860 0.000711 0.002511 0.000900 0.000845 0.005133 0.003112 0.000489 0.002550
Eisosomes 0.000024 0.000026 0.000043 0.000028 0.000015 0.000024 0.000049 0.000027 0.000019 0.000025 0.000045 0.000028
Endoplasmic Reticulum 0.000931 0.004532 0.007761 0.005350 0.001827 0.004034 0.006673 0.004269 0.001439 0.004236 0.007375 0.005184
Endosome 0.000932 0.001210 0.000447 0.002618 0.000515 0.001591 0.000675 0.003770 0.000696 0.001436 0.000528 0.002795
Golgi 0.000283 0.001060 0.000119 0.003072 0.000105 0.001267 0.000074 0.002808 0.000182 0.001183 0.000103 0.003032
Lipid Particles 0.000942 0.001669 0.000458 0.000568 0.000225 0.000856 0.000725 0.000733 0.000536 0.001187 0.000553 0.000593
Mitochondria 0.001822 0.004385 0.001201 0.005321 0.000957 0.007423 0.000974 0.002094 0.001332 0.006186 0.001121 0.004824
Mitotic Spindle 0.001075 0.012180 0.000825 0.028859 0.001365 0.016215 0.001002 0.046762 0.001240 0.014572 0.000888 0.031614
None 0.026578 0.013672 0.004143 0.012590 0.004976 0.006982 0.005734 0.016890 0.014338 0.009706 0.004707 0.013252
Nuclear Periphery 0.000556 0.000959 0.000539 0.002604 0.000592 0.002208 0.000950 0.001616 0.000576 0.001700 0.000685 0.002452
Nuclear Periphery Foci 0.000760 0.001342 0.000416 0.000990 0.000455 0.000790 0.000363 0.000491 0.000587 0.001015 0.000397 0.000913
Nucleolus 0.008390 0.020859 0.007956 0.001229 0.026706 0.020629 0.003817 0.003644 0.018768 0.020723 0.006488 0.001600
Nucleus 0.801968* 0.884277* 0.947868* 0.704171* 0.949889* 0.890834* 0.922677* 0.762116* 0.885787* 0.888164* 0.938934* 0.713085*
Peroxisomes 0.000911 0.001737 0.000353 0.000262 0.000202 0.001188 0.000309 0.000437 0.000509 0.001412 0.000337 0.000289
Vacuole 0.002493 0.002262 0.004983 0.022832 0.000404 0.004446 0.015460 0.018551 0.001309 0.003557 0.008699 0.022174
Vacuole Periphery 0.000861 0.000582 0.000205 0.001863 0.000184 0.001067 0.000153 0.003530 0.000478 0.000870 0.000186 0.002120

Localization Changes (T-score)

R1 R2 R1 & R2
UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14 UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14 UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14
Actin -12.76 -9.77 -4.97 -1.71 2.17 -6.64 -8.68 -7.74 -4.11 -0.58 -11.88 -12.03 -5.69 -2.56 1.72
Bud Neck -6.99 -7.11 -8.09 -3.02 -0.84 -11.43 -2.64 -4.02 1.50 -0.65 -13.32 -7.00 -8.79 -0.78 -1.32
Bud Site -1.63 -1.91 -6.08 -5.66 -5.58 -7.02 -2.69 -3.77 -0.99 -0.89 -6.22 -3.99 -6.95 -5.29 -5.37
Cell Periphery 1.38 1.70 0.60 -3.08 -3.87 -3.99 -3.74 -6.46 -2.37 -1.10 0.34 0.41 -1.64 -4.32 -4.00
Cytoplasm 17.49 24.26 2.15 -16.38 -24.07 -7.49 -4.04 -8.74 -7.09 -6.48 13.23 17.94 -10.47 -20.50 -23.61
Cytoplasmic Foci 5.25 9.61 7.89 2.38 -6.08 -3.26 0.24 -0.78 1.95 -0.93 3.29 9.31 5.95 2.36 -5.85
Eisosomes -1.31 -5.07 -6.36 -5.17 2.28 -5.47 -8.00 -8.94 -6.40 0.48 -4.75 -8.99 -12.74 -8.11 2.93
Endoplasmic Reticulum -13.83 -10.06 -20.23 -7.97 1.13 -7.25 -9.14 -8.40 -5.36 -0.29 -13.22 -12.76 -20.52 -10.12 1.09
Endosome -1.02 1.12 -2.69 -1.60 -3.88 -3.13 -1.94 -2.04 -0.47 -1.26 -3.18 -0.71 -3.93 -1.27 -3.83
Golgi -2.00 1.15 -1.41 -0.22 -1.59 -3.01 -1.06 -1.22 0.66 -1.15 -3.58 0.44 -1.71 -0.05 -1.75
Lipid Particles -1.13 2.18 1.67 1.47 -1.13 -2.19 -4.77 -2.06 -0.63 -0.73 -2.05 -1.43 -2.84 1.11 -1.16
Mitochondria -2.70 1.08 -2.09 1.09 -3.53 -5.73 -1.06 -2.52 5.03 -0.79 -6.25 0.01 -3.25 3.46 -3.46
Mitotic Spindle -4.78 -0.95 -7.26 -2.46 -6.29 -6.35 -1.59 -3.69 -1.95 -3.03 -7.96 -1.75 -8.09 -2.79 -6.61
None 5.28 10.83 8.03 2.84 -5.90 -2.32 -0.23 -5.20 -3.87 -4.74 3.73 9.20 3.67 -0.14 -6.88
Nuclear Periphery -4.40 -1.59 -13.39 -10.63 -12.90 -3.19 -4.85 -8.99 -1.95 -5.06 -3.80 -4.24 -14.51 -5.19 -12.34
Nuclear Periphery Foci -1.94 -0.39 -2.32 0.31 -0.87 -1.81 -1.62 -2.32 -1.36 -1.05 -2.59 -1.11 -3.87 -1.25 -1.30
Nucleolus -7.42 -0.63 10.87 12.33 8.66 2.55 9.90 9.65 7.38 0.57 -2.28 9.19 19.67 18.06 7.17
Nucleus -8.81 -16.54 5.32 13.93 21.49 11.56 4.21 10.55 6.15 6.53 0.37 -7.00 17.51 16.86 21.04
Peroxisomes -1.73 1.46 2.01 3.53 2.58 -3.48 -1.11 -0.90 1.04 -0.77 -3.31 0.98 1.30 4.44 0.77
Vacuole -0.11 -3.23 -14.54 -14.37 -10.77 -7.24 -4.81 -6.75 -5.97 -2.39 -5.97 -5.97 -16.47 -15.12 -8.82
Vacuole Periphery 0.65 1.64 -0.06 -0.90 -2.17 -4.15 0.41 -0.94 0.28 -0.97 -1.77 1.62 -1.21 -0.06 -2.41
Standard name
Human Ortholog
Description Subunit of the SAGA transcriptional regulatory complex; not present in SAGA-like complex SLIK/SALSA; required for SAGA-mediated inhibition at some promoters
Localization
Cell Percentages nucleus (93%)
Cell Cycle Regulation No
Subcompartmental Group nucleus-4

Spt8

Spt8


Nearest neighbours ( genes)

correlation threshold

0.6

1.0

0.80


Biological Process (Gene Ontology)
Molecular Function (Gene Ontology)
Cellular Component (Gene Ontology)

Endocytosis
Temp Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Vph1-GFP)
37℃
RT
Cell Cycle Omics

CYCLoPs (Spt8-GFP)
Gene / Allele Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Sac6-tdTomato)
Gene Images
Gene Images
Images are not yet available
Images are not yet available