Standard name
Human Ortholog
Description Beta subunit of cytoplasmic phenylalanyl-tRNA synthetase; forms a tetramer with Frs2p to generate active enzyme; able to hydrolyze mis-aminoacylated tRNA-Phe, which could contribute to translational quality control
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Micrographs

ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 0 0 0.05 0 0.05 0.07 – 0 0 0 0 0 0 0 0 0 0 0
Bud 0 0 0 0 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0.05 0 0
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 – 0 0 0 0 0 – – – – – –
Cell Periphery 0 0 0 0 0 0.05 0.08 0.07 0.07 0.17 0 0.12 – 0 0.06 0 0 0 0 0 0 0 0 0
Cytoplasm 0.99 1.0 0.97 0.98 0.98 0.94 0.84 0.82 0.84 0.83 0.8 0.79 – 1.0 0.97 0.97 0.98 0.98 0.96 0.96 0.9 0.94 0.84 0.89
Endoplasmic Reticulum 0 0 0.07 0 0 0.05 0.11 0.13 0.06 0.07 0.05 0.05 – 0 0 0 0.05 0.06 0 0 0.05 0 0 0
Endosome 0 0 0 0 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0
Golgi 0 0 0 0 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0
Mitochondria 0 0 0 0 0 0 0 0 0 0 0.11 0.07 – 0 0 0 0 0 0 0 0 0 0 0
Nucleus 0 0 0 0 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0
Nuclear Periphery 0 0 0 0 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 0 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0
Vac/Vac Membrane 0 0 0 0 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0
ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 1 5 3 6 1 2 6 – 0 0 0 0 0 0 0 0 0 0 0
Bud 0 0 0 0 0 1 0 0 0 0 0 0 – 0 0 0 0 0 0 1 0 0 0 1
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 – 0 0 0 0 0 – – – – – –
Cell Periphery 0 4 2 0 2 5 13 17 9 7 1 10 – 1 6 10 5 8 0 0 0 0 0 0
Cytoplasm 86 131 59 46 63 104 144 192 102 35 35 63 – 44 106 367 254 312 97 122 62 16 36 52
Endoplasmic Reticulum 0 1 4 0 1 5 19 31 7 3 2 4 – 0 0 11 13 18 1 0 3 0 0 0
Endosome 0 0 0 0 0 0 0 0 0 0 0 1 – 0 0 0 0 0 0 0 0 0 0 0
Golgi 1 0 0 0 0 0 4 1 1 0 0 0 – 0 0 3 1 0 0 0 0 0 0 0
Mitochondria 1 0 0 0 0 1 0 0 1 0 5 6 – 0 0 3 1 1 1 0 0 0 0 0
Nucleus 0 0 0 0 0 0 0 0 1 0 1 0 – 0 1 0 0 0 0 0 0 0 1 0
Nuclear Periphery 0 0 0 0 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 0 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 1 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0
Vac/Vac Membrane 0 0 0 1 0 0 1 1 1 1 0 2 – 0 2 2 2 6 0 0 0 0 0 1
Unique Cell Count 87 131 61 47 64 111 171 233 121 42 44 80 44 109 378 260 319 101 128 70 17 43 59
Labelled Cell Count 88 136 65 47 66 117 186 245 128 47 46 92 45 115 396 276 345 101 128 70 17 43 59


Cytoplasm

(Huh et al. Nature 2003 425:686)
Screen WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 AF100 AF140 AF180
Mean Cell GFP Intensity (1e-4) 45.0 57.6 54.9 50.5 42.7 38.0 34.7 33.2 28.0 29.3 24.1 24.2 – 45.5 46.9 71.3 71.8 61.4 – 57.6 62.1
Std Deviation (1e-4) 10.9 15.1 8.2 15.0 9.2 9.3 7.6 7.7 7.1 7.8 6.7 6.4 – 23.0 19.1 17.0 14.4 14.3 – 20.0 20.7
Intensity Change (Log2) – – – -0.12 -0.36 -0.53 -0.66 -0.73 -0.97 -0.91 -1.19 -1.18 – -0.27 -0.23 0.38 0.39 0.16 – 0.07 0.18


Localization RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3
Cortical Patches 0 0 0 0 0 0 0 0 0 – 0 0 0 0 0
Bud 0 0 0 0 0 0 0 0 0 – 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 0 0 – 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 – 0 0 0 0 0
Cell Periphery 0 0 0 0 0 0 0 0 1.9 – 0 0 0 0 0
Cytoplasm 0.4 0.6 -0.9 -2.5 -2.8 -2.5 -2.4 -2.8 -3.1 – 1.2 0.2 0.2 0.4 0.5
Endoplasmic Reticulum 0 0 0 1.0 1.4 0 0 0 0 – 0 0 0 0 0
Endosome 0 0 0 0 0 0 0 0 0 – 0 0 0 0 0
Golgi 0 0 0 0 0 0 0 0 0 – 0 0 0 0 0
Mitochondria 0 0 0 0 0 0 0 0 0 – 0 0 0 0 0
Nucleus 0 0 0 0 0 0 0 0 0 – 0 0 0 0 0
Nuclear Periphery 0 0 0 0 0 0 0 0 0 – 0 0 0 0 0
Nucleolus 0 0 0 0 0 0 0 0 0 – 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 0 0 – 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 0 0 – 0 0 0 0 0
Vacuole 0 0 0 0 0 0 0 0 0 – 0 0 0 0 0

Images

Circle plot not available

Protein Concentration and Protein Localization Data

R1 R2 R3
G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase
Concentration 30.2524 49.5518 43.7623 43.6937 38.5222 44.6122 30.0184 43.9742 44.2713 40.5518 33.1801 46.1874 30.478 52.2519 43.1437 42.9229 47.1331 47.1094
Actin 0.0193 0.0004 0.009 0.012 0.0014 0.001 0.0358 0.0023 0.0052 0.0011 0.0015 0.0041 0.0058 0.0009 0.0177 0.0001 0.034 0.0217
Bud 0.002 0.0012 0.0014 0.0008 0.0042 0.0008 0.0019 0.0006 0.0028 0.0017 0.0202 0.0019 0.0008 0.0002 0.0021 0.002 0.001 0.0006
Bud Neck 0.0024 0.0004 0.001 0.001 0.0007 0.0023 0.0007 0.0004 0.0006 0.0007 0.0008 0.0022 0.0003 0.0001 0.0008 0.0003 0.0007 0.0027
Bud Periphery 0.0024 0.0007 0.0018 0.0006 0.0048 0.0007 0.0026 0.0014 0.0029 0.0011 0.0103 0.0021 0.0013 0 0.0048 0.001 0.001 0.0002
Bud Site 0.0039 0.0009 0.0013 0.0003 0.0007 0.0001 0.0022 0.0035 0.0009 0.001 0.0033 0.0016 0.001 0.0003 0.0034 0.0002 0.0012 0.0002
Cell Periphery 0.0058 0.0056 0.0108 0.0143 0.0014 0.0017 0.0018 0.0027 0.0007 0.003 0.0015 0.0004 0.0076 0.0003 0.0008 0.0002 0.0001 0.0003
Cytoplasm 0.6564 0.9455 0.8349 0.7464 0.7612 0.863 0.6765 0.9723 0.903 0.7915 0.3689 0.9243 0.7146 0.9877 0.8689 0.8417 0.8706 0.9269
Cytoplasmic Foci 0.021 0.0013 0.0087 0.0025 0.0104 0.0101 0.0091 0.0012 0.0034 0.0039 0.0109 0.0072 0.0469 0.0013 0.0108 0.0023 0.012 0.0061
Eisosomes 0.0003 0 0.0002 0.0001 0 0 0.0003 0.0002 0 0 0 0 0.0003 0 0.0007 0 0.0002 0
Endoplasmic Reticulum 0.0309 0.0164 0.0156 0.0288 0.013 0.0235 0.0271 0.0017 0.015 0.0034 0.2058 0.004 0.0291 0.0035 0.0057 0.0008 0.0009 0.0099
Endosome 0.0297 0.0018 0.0149 0.0105 0.0365 0.0244 0.0208 0.0012 0.0178 0.0459 0.0509 0.0236 0.0389 0.0011 0.0199 0.0327 0.0182 0.0117
Golgi 0.0072 0.0003 0.0042 0.0088 0.0037 0.0047 0.0045 0.0004 0.0036 0.0168 0.0038 0.0085 0.0051 0.0004 0.0022 0.0013 0.0172 0.0016
Lipid Particles 0.008 0.0004 0.0037 0.0037 0.0009 0.001 0.0029 0.0002 0.0002 0.0009 0.0009 0.0007 0.0167 0.0001 0.0012 0.0001 0.0054 0.0002
Mitochondria 0.0248 0.0005 0.015 0.0039 0.0423 0.016 0.0126 0.0059 0.016 0.0124 0.034 0.0027 0.0113 0.0001 0.0101 0.002 0.0243 0.0006
None 0.0078 0.0003 0.0005 0.0002 0.0012 0.0008 0.006 0.0002 0.0003 0.0004 0.0014 0.0002 0.0129 0.0002 0.0021 0.0002 0.0004 0.0008
Nuclear Periphery 0.0244 0.0025 0.0038 0.0064 0.0236 0.0028 0.0885 0.0006 0.0062 0.0063 0.0088 0.0018 0.0175 0.0007 0.0073 0.0018 0.0019 0.0015
Nucleolus 0.0056 0.0001 0.0004 0.0001 0.001 0.0007 0.0006 0 0.0001 0.0008 0.0022 0.0001 0.0015 0 0.001 0.0001 0.0002 0
Nucleus 0.0232 0.0016 0.0025 0.0026 0.0079 0.0022 0.046 0.0008 0.0023 0.008 0.0108 0.0017 0.0053 0.0007 0.0037 0.0017 0.0012 0.002
Peroxisomes 0.0031 0 0.0008 0.0008 0.0011 0.0001 0.0018 0 0.0001 0.0001 0.002 0.0002 0.0014 0 0.0004 0.0001 0.0038 0.0005
Punctate Nuclear 0.0139 0 0.0005 0.0001 0.0003 0.008 0.0062 0 0.0001 0.0001 0.0002 0.0002 0.0216 0 0.0004 0 0.0009 0.0002
Vacuole 0.093 0.0188 0.0623 0.1343 0.0613 0.0249 0.0393 0.0039 0.0125 0.0781 0.1754 0.0103 0.0466 0.0017 0.0248 0.0653 0.0033 0.0077
Vacuole Periphery 0.0146 0.0013 0.0066 0.0217 0.0225 0.0111 0.0126 0.0005 0.006 0.0226 0.0866 0.0024 0.0135 0.0003 0.0111 0.0463 0.0016 0.0046

Sequencing Data

R1 R2
G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Post-START S/G2 Metaphase Anaphase Telophase
Gene Expression 203.957 186.4626 187.8957 221.3541 204.8304 163.4454 180.0259 179.3874 173.3251 193.5075
Translational Efficiency 1.3739 1.5343 1.4951 1.143 1.2044 1.5758 1.415 1.2349 1.2887 1.2335

Hit Data

Dataset Hit
Protein Concentration ✘
Protein Localization ✘
Gene Expression ✘
Translational Efficiency ✘

Micrographs

Cell Count

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
2265 1049 2922 106 1947 2294 1422 3364 4212 3343 4344 3470

Protein Abundance

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
Mean 3013.27 2811.17 4616.88 5687.31 3418.13 3630.62 4363.93 5213.26 3200.42 3373.48 4534.08 5227.74
Standard Deviation 622.23 736.24 767.17 932.51 577.97 720.50 873.34 929.79 635.10 819.09 812.19 933.45
Intensity Change Log 2 — -0.100159 0.615588 0.916416 — 0.087009 0.352421 0.608979 — 0.002329 0.481713 0.761199

Localization Score (DeepLoc)

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
Actin 0.000025 0.000078 0.000052 0.000306 0.000041 0.000046 0.000159 0.000028 0.000032 0.000056 0.000087 0.000036
Bud Neck 0.000069 0.000329 0.000434 0.003746 0.000162 0.000262 0.002638 0.000506 0.000112 0.000283 0.001156 0.000605
Bud Site 0.002166 0.003373 0.003077 0.008243 0.002228 0.002477 0.005504 0.005471 0.002194 0.002758 0.003871 0.005556
Cell Periphery 0.001606 0.004777 0.003068 0.104357* 0.003602 0.002226 0.009594 0.000590 0.002529 0.003026 0.005204 0.003760
Cytoplasm 0.916956* 0.856303* 0.769440* 0.315386* 0.897563* 0.915069* 0.681406* 0.702981* 0.907992* 0.896629* 0.740622* 0.691141*
Cytoplasmic Foci 0.008523 0.007135 0.004744 0.007477 0.006668 0.005235 0.008677 0.004410 0.007666 0.005831 0.006032 0.004504
Eisosomes 0.000000 0.000001 0.000001 0.000015 0.000001 0.000001 0.000003 0.000001 0.000000 0.000001 0.000001 0.000001
Endoplasmic Reticulum 0.000579 0.000654 0.000491 0.000167 0.000612 0.000370 0.000892 0.000302 0.000594 0.000459 0.000623 0.000298
Endosome 0.000348 0.000704 0.000619 0.000526 0.000495 0.000555 0.001380 0.000953 0.000416 0.000602 0.000868 0.000940
Golgi 0.018381 0.035149 0.011432 0.021746 0.029622 0.018174 0.022062 0.002997 0.023577 0.023501 0.014912 0.003570
Lipid Particles 0.000807 0.005594 0.004195 0.055855 0.004078 0.002635 0.014479 0.000638 0.002319 0.003563 0.007561 0.002325
Mitochondria 0.000178 0.000112 0.000250 0.000048 0.000129 0.000173 0.000146 0.000567 0.000155 0.000154 0.000216 0.000551
Mitotic Spindle 0.000656 0.000549 0.000587 0.000543 0.000799 0.001631 0.002161 0.000149 0.000722 0.001291 0.001103 0.000161
None 0.001653 0.005720 0.000534 0.000660 0.001161 0.001633 0.002064 0.001094 0.001426 0.002915 0.001035 0.001081
Nuclear Periphery 0.000074 0.000174 0.000182 0.001082 0.000123 0.000082 0.000743 0.000033 0.000097 0.000111 0.000366 0.000065
Nuclear Periphery Foci 0.002755 0.009660 0.003261 0.009162 0.005419 0.003509 0.012058 0.000426 0.003987 0.005439 0.006141 0.000693
Nucleolus 0.000034 0.000119 0.000121 0.000127 0.000048 0.000042 0.000120 0.000275 0.000041 0.000066 0.000121 0.000271
Nucleus 0.002467 0.010463 0.006245 0.011723 0.002605 0.006288 0.027438 0.008947 0.002530 0.007598 0.013182 0.009032
Peroxisomes 0.000014 0.000025 0.000049 0.000042 0.000022 0.000020 0.000027 0.000153 0.000018 0.000021 0.000042 0.000149
Vacuole 0.008382 0.032451 0.151417 0.436213* 0.016681 0.024059 0.177487 0.228602* 0.012219 0.026692 0.159951 0.234944*
Vacuole Periphery 0.034327 0.026630 0.039800 0.022575 0.027941 0.015514 0.030961 0.040876 0.031375 0.019002 0.036906 0.040317

Localization Changes (T-score)

R1 R2 R1 & R2
UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14 UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14 UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14
Actin -6.99 -3.30 -5.51 -4.56 -4.81 -0.97 -11.97 1.75 2.75 12.79 -5.20 -8.99 -1.26 2.87 7.50
Bud Neck -7.84 -9.71 -6.34 -5.95 -5.48 -2.75 -13.31 -8.79 -4.66 11.82 -5.88 -15.47 -12.13 -6.87 9.26
Bud Site -2.91 -3.39 -3.41 -2.82 -2.90 -0.88 -6.64 -7.96 -6.42 2.12 -2.18 -7.37 -8.86 -6.77 -2.43
Cell Periphery -6.57 -4.68 -9.73 -9.40 -9.59 3.31 -6.32 8.65 7.19 10.96 -1.74 -6.87 -2.21 -1.12 2.73
Cytoplasm 7.86 24.83 18.74 16.54 13.65 -2.80 25.35 28.52 33.68 -5.42 2.98 34.26 36.41 33.06 4.28
Cytoplasmic Foci 1.54 4.65 -0.57 -1.17 -2.14 2.52 -4.48 4.96 2.77 7.68 3.65 1.28 7.23 4.00 5.61
Eisosomes -6.57 -7.15 -7.44 -7.13 -7.12 0.60 -11.62 -1.68 -2.78 10.48 -3.12 -12.21 -6.04 -4.71 2.77
Endoplasmic Reticulum -0.59 1.20 5.49 4.05 7.33 2.79 -1.39 4.53 1.36 3.49 1.81 -0.11 5.74 3.09 5.16
Endosome -5.01 -6.61 -2.61 1.85 1.54 -0.79 -7.31 -6.60 -4.51 4.23 -3.23 -9.14 -9.89 -4.69 0.17
Golgi -6.24 5.38 -0.66 3.38 -2.65 5.52 4.73 15.76 12.29 17.83* 0.05 8.25 19.50 17.25 21.21*
Lipid Particles -7.15 -10.40 -7.74 -7.01 -7.23 2.36 -9.16 6.43 6.88 13.62 -3.38 -11.23 0.00 3.26 10.96
Mitochondria 2.83 -1.71 6.27 3.43 4.74 -1.26 -2.10 -4.19 -3.64 -3.73 0.03 -2.39 -3.93 -3.88 -3.04
Mitotic Spindle 0.65 -0.20 0.54 -0.00 0.87 -1.51 -4.08 2.64 2.74 7.17 -1.53 -2.80 3.84 3.01 8.16
None -4.55 9.25 5.14 5.61 -0.77 -1.26 -1.06 0.25 1.12 1.07 -3.90 1.77 1.07 3.68 -0.15
Nuclear Periphery -5.22 -6.23 -8.03 -7.19 -6.83 2.87 -10.35 6.61 5.02 11.77 -1.61 -11.67 2.27 4.05 12.65
Nuclear Periphery Foci -7.62 -2.30 -5.02 0.41 -4.39 3.51 -7.03 10.77 10.13 13.49 -3.58 -5.84 12.01 13.22 16.19
Nucleolus -3.65 -7.38 -5.34 -0.39 0.11 0.24 -4.00 -9.23 -23.24* -11.46 -1.84 -7.04 -15.55 -18.19 -13.16
Nucleus -7.87 -9.30 -4.06 -1.25 -1.94 -8.08 -14.62 -13.60 -5.37 11.31 -11.37 -16.81 -14.56 -4.03 7.43
Peroxisomes -2.12 -9.86 -3.83 -1.95 0.68 0.45 -2.08 -13.86 -14.82 -12.32 -1.46 -8.34 -15.15 -14.58 -11.86
Vacuole -9.47 -36.45 -16.43 -15.42 -10.42 -4.35 -26.05 -45.48 -42.90 -3.93 -10.45 -44.25 -49.13 -44.52 -10.80
Vacuole Periphery 2.63 -1.64 3.64 1.46 5.31 6.04 0.17 -4.93 -14.09 -5.33 7.35 -1.97 -3.73 -12.11 -2.14
Standard name
Human Ortholog
Description Beta subunit of cytoplasmic phenylalanyl-tRNA synthetase; forms a tetramer with Frs2p to generate active enzyme; able to hydrolyze mis-aminoacylated tRNA-Phe, which could contribute to translational quality control
Localization
Cell Percentages cytoplasm (100%)
Cell Cycle Regulation No
Subcompartmental Group cytoplasm-2

Frs1

Frs1


Nearest neighbours ( genes)

correlation threshold

0.6

1.0

0.80


Biological Process (Gene Ontology)
Molecular Function (Gene Ontology)
Cellular Component (Gene Ontology)

Endocytosis
Temp Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Vph1-GFP)
37℃
RT
Cell Cycle Omics

CYCLoPs (Frs1-GFP)
Gene / Allele Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Sac6-tdTomato)
Gene Images
Gene Images
Images are not yet available
Images are not yet available