Standard name | |
---|---|
Human Ortholog | |
Description | Ribosomal 60S subunit protein L22A; required for translation of long 5' UTR of IME1 mRNA and meiotic entry; required for the oxidative stress response, pseudohyphal and invasive growth; homologous to mammalian ribosomal protein L22, no bacterial homolog; RPL22A has a paralog, RPL22B, that arose from the whole genome duplication |
ensLOC | DeepLoc | |||||||||||||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Localization | WT1 | WT2 | WT3 | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 | WT1 | WT2 | WT3 | AF100 | AF140 | AF180 |
Cortical Patches | – | 0 | 0 | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 0 | 0 | 0 | 0 |
Bud | – | 1 | 0 | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 1 | 0 | 0 | 1 | 0 | 0 | 0 | 0 | 1 | 0 | 0 | 3 | 0 |
Bud Neck | – | 0 | 0 | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Bud Site | – | 0 | 0 | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | – | – | – | – | – | – |
Cell Periphery | – | 0 | 2 | 0 | 1 | 0 | 0 | – | 0 | 0 | 3 | 6 | 0 | 1 | 0 | 0 | 7 | 6 | 0 | 0 | 0 | 0 | 0 | 0 |
Cytoplasm | – | 279 | 122 | 36 | 68 | 37 | 56 | – | 49 | 32 | 117 | 102 | 103 | 131 | 183 | 188 | 86 | 157 | 26 | 270 | 114 | 134 | 104 | 119 |
Endoplasmic Reticulum | – | 1 | 0 | 0 | 1 | 1 | 3 | – | 8 | 7 | 15 | 18 | 0 | 2 | 1 | 14 | 8 | 13 | 0 | 2 | 3 | 3 | 2 | 7 |
Endosome | – | 0 | 0 | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 0 | 0 | 0 | 0 |
Golgi | – | 2 | 0 | 0 | 0 | 0 | 0 | – | 0 | 0 | 1 | 2 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 2 | 0 |
Mitochondria | – | 0 | 0 | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 2 | 0 | 0 | 0 | 0 | 0 | 0 |
Nucleus | – | 0 | 0 | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 1 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 0 | 0 | 0 | 0 |
Nuclear Periphery | – | 0 | 0 | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 0 | 0 |
Nucleolus | – | 0 | 0 | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Peroxisomes | – | 0 | 0 | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
SpindlePole | – | 0 | 0 | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Vac/Vac Membrane | – | 1 | 0 | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 1 | 0 | 1 | 2 | 0 | 1 | 0 | 1 | 8 | 0 | 2 | 0 | 1 |
Unique Cell Count | 283 | 123 | 36 | 70 | 37 | 59 | 57 | 39 | 135 | 128 | 104 | 134 | 185 | 188 | 88 | 161 | 29 | 296 | 122 | 148 | 120 | 134 | ||
Labelled Cell Count | 284 | 124 | 36 | 70 | 38 | 59 | 57 | 39 | 136 | 132 | 104 | 135 | 187 | 202 | 102 | 178 | 29 | 296 | 122 | 148 | 120 | 134 |
Screen | WT1 | WT2 | WT3 | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 | AF100 | AF140 | AF180 |
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Mean Cell GFP Intensity (1e-4) | – | 274.7 | 308.2 | 258.4 | 168.0 | 182.3 | 149.7 | – | 126.1 | 109.9 | 113.3 | 100.4 | 278.9 | 267.9 | 265.7 | 303.7 | 412.2 | 410.6 | 306.3 | 318.1 | 315.9 |
Std Deviation (1e-4) | – | 71.0 | 63.6 | 78.3 | 44.9 | 38.5 | 40.7 | – | 34.1 | 28.8 | 30.5 | 26.7 | 68.1 | 77.4 | 55.8 | 120.1 | 141.4 | 128.1 | 71.3 | 117.1 | 107.0 |
Intensity Change (Log2) | – | – | – | -0.25 | -0.88 | -0.76 | -1.04 | – | -1.29 | -1.49 | -1.44 | -1.62 | -0.14 | -0.2 | -0.21 | -0.02 | 0.42 | 0.41 | -0.01 | 0.05 | 0.04 |
Localization | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 |
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Cortical Patches | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Bud | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Bud Neck | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Bud Site | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Cell Periphery | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Cytoplasm | 0.5 | -1.1 | 0.6 | -1.8 | – | -3.8 | -4.3 | -3.8 | -5.0 | -0.1 | -0.9 | -0.2 | 1.2 | -0.9 | -1.1 |
Endoplasmic Reticulum | 0 | 0 | 0 | 0 | – | 0 | 0 | 3.8 | 4.3 | 0 | 0 | 0 | 3.1 | 0 | 3.2 |
Endosome | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Golgi | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Mitochondria | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Nucleus | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Nuclear Periphery | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Nucleolus | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Peroxisomes | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
SpindlePole | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Vacuole | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
R1 | R2 | R3 | ||||||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | |
Concentration | 247.4749 | 346.2064 | 287.7575 | 358.7399 | 266.1671 | 222.7764 | 266.2495 | 400.9431 | 343.2707 | 426.4071 | 374.3448 | 332.1406 | 277.2535 | 374.8028 | 330.7038 | 334.4824 | 292.2025 | 293.0423 |
Actin | 0.0235 | 0.0036 | 0.0119 | 0.0063 | 0.0066 | 0.0149 | 0.0088 | 0.0023 | 0.0028 | 0.0015 | 0.0025 | 0.0128 | 0.0089 | 0.0018 | 0.0057 | 0.0027 | 0.0037 | 0.046 |
Bud | 0.0068 | 0.0073 | 0.0092 | 0.0107 | 0.0196 | 0.0149 | 0.0067 | 0.0044 | 0.0066 | 0.0053 | 0.0055 | 0.003 | 0.0106 | 0.0061 | 0.0137 | 0.0221 | 0.015 | 0.0118 |
Bud Neck | 0.0019 | 0.0016 | 0.0035 | 0.0098 | 0.0017 | 0.006 | 0.0025 | 0.0016 | 0.0026 | 0.0028 | 0.0027 | 0.0064 | 0.0019 | 0.0014 | 0.0036 | 0.0016 | 0.0037 | 0.0106 |
Bud Periphery | 0.0102 | 0.0077 | 0.01 | 0.0085 | 0.0092 | 0.0134 | 0.0103 | 0.0042 | 0.0051 | 0.0026 | 0.0042 | 0.0049 | 0.0099 | 0.0033 | 0.0081 | 0.0089 | 0.0187 | 0.0147 |
Bud Site | 0.0037 | 0.0079 | 0.0099 | 0.0021 | 0.0045 | 0.0033 | 0.0038 | 0.0055 | 0.0045 | 0.0011 | 0.0009 | 0.001 | 0.0056 | 0.0044 | 0.0056 | 0.0016 | 0.006 | 0.0026 |
Cell Periphery | 0.1354 | 0.086 | 0.1391 | 0.0505 | 0.0411 | 0.0809 | 0.1229 | 0.147 | 0.1466 | 0.0461 | 0.0569 | 0.0828 | 0.0951 | 0.07 | 0.0692 | 0.146 | 0.1235 | 0.0368 |
Cytoplasm | 0.2395 | 0.6099 | 0.2789 | 0.3466 | 0.2448 | 0.1546 | 0.2689 | 0.4591 | 0.3769 | 0.5888 | 0.262 | 0.2656 | 0.3076 | 0.7022 | 0.4317 | 0.5045 | 0.0788 | 0.2258 |
Cytoplasmic Foci | 0.0116 | 0.0019 | 0.0053 | 0.0028 | 0.0048 | 0.0258 | 0.0112 | 0.0012 | 0.0059 | 0.001 | 0.0019 | 0.0109 | 0.0088 | 0.0013 | 0.0037 | 0.001 | 0.0018 | 0.0036 |
Eisosomes | 0.0048 | 0.0005 | 0.0009 | 0.0001 | 0.0012 | 0.0036 | 0.0086 | 0.0004 | 0.0014 | 0 | 0.001 | 0.0026 | 0.0026 | 0.0031 | 0.0025 | 0.0011 | 0.0004 | 0.0019 |
Endoplasmic Reticulum | 0.0373 | 0.0094 | 0.0412 | 0.0085 | 0.0055 | 0.016 | 0.0197 | 0.0087 | 0.0055 | 0.0019 | 0.0091 | 0.025 | 0.0097 | 0.004 | 0.0023 | 0.0011 | 0.0013 | 0.0059 |
Endosome | 0.0068 | 0.0026 | 0.0144 | 0.0071 | 0.0045 | 0.0353 | 0.0053 | 0.002 | 0.0053 | 0.0017 | 0.0087 | 0.0052 | 0.0038 | 0.0011 | 0.0052 | 0.0013 | 0.0034 | 0.0069 |
Golgi | 0.0021 | 0.0008 | 0.0032 | 0.0015 | 0.0007 | 0.0065 | 0.0018 | 0.0004 | 0.0006 | 0.0001 | 0.0031 | 0.0025 | 0.0011 | 0.0003 | 0.001 | 0.0003 | 0.0005 | 0.0052 |
Lipid Particles | 0.0055 | 0.0023 | 0.0046 | 0.0021 | 0.0061 | 0.007 | 0.0094 | 0.0024 | 0.0049 | 0.0011 | 0.0107 | 0.0054 | 0.0047 | 0.0017 | 0.0046 | 0.0057 | 0.0059 | 0.0037 |
Mitochondria | 0.0718 | 0.0254 | 0.051 | 0.0129 | 0.0858 | 0.1806 | 0.0565 | 0.0121 | 0.0495 | 0.0041 | 0.0798 | 0.1444 | 0.115 | 0.018 | 0.0579 | 0.0107 | 0.047 | 0.1158 |
None | 0.0157 | 0.0024 | 0.0037 | 0.0013 | 0.005 | 0.0153 | 0.0188 | 0.0021 | 0.0083 | 0.0015 | 0.0015 | 0.008 | 0.0088 | 0.0032 | 0.0115 | 0.0021 | 0.0016 | 0.0058 |
Nuclear Periphery | 0.0771 | 0.023 | 0.055 | 0.0177 | 0.0903 | 0.0653 | 0.0678 | 0.0178 | 0.047 | 0.0228 | 0.029 | 0.0946 | 0.0662 | 0.0139 | 0.0218 | 0.013 | 0.0078 | 0.0554 |
Nucleolus | 0.0283 | 0.0111 | 0.0257 | 0.005 | 0.0556 | 0.0466 | 0.0343 | 0.0126 | 0.043 | 0.007 | 0.0117 | 0.0546 | 0.0331 | 0.0145 | 0.0696 | 0.0257 | 0.0934 | 0.0706 |
Nucleus | 0.0549 | 0.0337 | 0.0427 | 0.0236 | 0.089 | 0.0639 | 0.0629 | 0.0422 | 0.0561 | 0.0485 | 0.0558 | 0.0527 | 0.0902 | 0.0328 | 0.0793 | 0.0473 | 0.0476 | 0.1054 |
Peroxisomes | 0.0012 | 0.0008 | 0.0012 | 0.0008 | 0.0034 | 0.0024 | 0.0018 | 0.0005 | 0.0013 | 0.0004 | 0.0009 | 0.0015 | 0.0017 | 0.0009 | 0.0021 | 0.0006 | 0.002 | 0.0031 |
Punctate Nuclear | 0.0008 | 0.0002 | 0.001 | 0.0002 | 0.0005 | 0.0017 | 0.0014 | 0.0001 | 0.0046 | 0.0002 | 0.0001 | 0.0011 | 0.0008 | 0.0001 | 0.0129 | 0.0001 | 0.0001 | 0.0007 |
Vacuole | 0.1883 | 0.1251 | 0.2002 | 0.3217 | 0.1639 | 0.119 | 0.1934 | 0.225 | 0.1704 | 0.2013 | 0.2459 | 0.1104 | 0.1184 | 0.0926 | 0.1067 | 0.1504 | 0.3267 | 0.0892 |
Vacuole Periphery | 0.0726 | 0.0369 | 0.0873 | 0.1599 | 0.1564 | 0.1232 | 0.0832 | 0.0484 | 0.0511 | 0.0603 | 0.2062 | 0.1046 | 0.0956 | 0.0235 | 0.0813 | 0.0522 | 0.2111 | 0.1781 |
R1 | R2 | |||||||||
---|---|---|---|---|---|---|---|---|---|---|
G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | |
Gene Expression | 2027.4303 | 2282.6793 | 2545.7693 | 2255.7754 | 2935.4909 | 2166.4169 | 1816.8463 | 1759.1405 | 1977.1567 | 1800.1048 |
Translational Efficiency | 1.8886 | 1.7573 | 1.8822 | 2.032 | 1.8143 | 1.8291 | 1.9805 | 2.6575 | 2.2065 | 2.394 |
Dataset | Hit |
---|---|
Protein Concentration | ✔ |
Protein Localization | ✔ |
Gene Expression | ✘ |
Translational Efficiency | ✘ |
R1 | R2 | R1 & R2 | |||||||||
---|---|---|---|---|---|---|---|---|---|---|---|
WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 |
395 | 693 | 1829 | 1120 | 2152 | 2179 | 1800 | 1281 | 2547 | 2872 | 3629 | 2401 |
R1 | R2 | R1 & R2 | ||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|
WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 | |
Mean | 9449.33 | 11964.21 | 22774.04 | 18852.03 | 13312.41 | 15753.27 | 24710.76 | 24277.69 | 12713.31 | 14838.99 | 23734.66 | 21746.77 |
Standard Deviation | 2259.59 | 3389.44 | 4298.61 | 5704.28 | 2734.76 | 3358.18 | 5102.14 | 5558.13 | 3011.04 | 3735.86 | 4812.73 | 6243.96 |
Intensity Change Log 2 | — | 0.340441 | 1.269106 | 0.996436 | — | 0.242880 | 0.892368 | 0.866859 | — | 0.284185 | 1.060855 | 0.922072 |
R1 | R2 | R1 & R2 | ||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|
WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 | |
Actin | 0.000090 | 0.000091 | 0.000044 | 0.000277 | 0.000015 | 0.000023 | 0.000047 | 0.000106 | 0.000027 | 0.000040 | 0.000046 | 0.000186 |
Bud Neck | 0.000875 | 0.000746 | 0.000637 | 0.003295 | 0.000185 | 0.000378 | 0.000888 | 0.001017 | 0.000292 | 0.000466 | 0.000762 | 0.002080 |
Bud Site | 0.004095 | 0.002968 | 0.001195 | 0.005462 | 0.000688 | 0.001245 | 0.001350 | 0.004978 | 0.001216 | 0.001661 | 0.001272 | 0.005204 |
Cell Periphery | 0.025377 | 0.012718 | 0.014972 | 0.002729 | 0.003752 | 0.002552 | 0.005471 | 0.025254 | 0.007106 | 0.005005 | 0.010260 | 0.014747 |
Cytoplasm | 0.544474* | 0.758669* | 0.716029* | 0.552804* | 0.763644* | 0.851958* | 0.784984* | 0.703591* | 0.729654* | 0.829448* | 0.750231* | 0.633253* |
Cytoplasmic Foci | 0.018730 | 0.010582 | 0.004509 | 0.014559 | 0.009841 | 0.006568 | 0.003829 | 0.004719 | 0.011219 | 0.007536 | 0.004172 | 0.009309 |
Eisosomes | 0.000003 | 0.000002 | 0.000001 | 0.000008 | 0.000000 | 0.000000 | 0.000001 | 0.000002 | 0.000001 | 0.000001 | 0.000001 | 0.000005 |
Endoplasmic Reticulum | 0.000560 | 0.000222 | 0.000352 | 0.000249 | 0.000580 | 0.000136 | 0.000146 | 0.000222 | 0.000577 | 0.000157 | 0.000250 | 0.000235 |
Endosome | 0.000758 | 0.000330 | 0.000219 | 0.002550 | 0.000382 | 0.000259 | 0.000210 | 0.000481 | 0.000441 | 0.000276 | 0.000214 | 0.001446 |
Golgi | 0.101880 | 0.025600 | 0.027107 | 0.007667 | 0.051552 | 0.021027 | 0.010783 | 0.011865 | 0.059357 | 0.022130 | 0.019010 | 0.009906 |
Lipid Particles | 0.017425 | 0.009321 | 0.006236 | 0.004193 | 0.003635 | 0.002324 | 0.004096 | 0.014967 | 0.005773 | 0.004012 | 0.005175 | 0.009941 |
Mitochondria | 0.000224 | 0.000294 | 0.000164 | 0.001784 | 0.000137 | 0.000145 | 0.000155 | 0.000152 | 0.000151 | 0.000181 | 0.000160 | 0.000913 |
Mitotic Spindle | 0.002298 | 0.000503 | 0.000545 | 0.008786 | 0.002485 | 0.001563 | 0.000340 | 0.001760 | 0.002456 | 0.001307 | 0.000443 | 0.005037 |
None | 0.003825 | 0.006983 | 0.001507 | 0.007400 | 0.001679 | 0.002957 | 0.001820 | 0.002019 | 0.002011 | 0.003928 | 0.001663 | 0.004529 |
Nuclear Periphery | 0.001350 | 0.000756 | 0.001320 | 0.001052 | 0.000926 | 0.000458 | 0.000679 | 0.000526 | 0.000991 | 0.000530 | 0.001002 | 0.000771 |
Nuclear Periphery Foci | 0.020337 | 0.009566 | 0.009017 | 0.007155 | 0.017309 | 0.007410 | 0.005927 | 0.006428 | 0.017779 | 0.007930 | 0.007485 | 0.006767 |
Nucleolus | 0.000511 | 0.000300 | 0.000077 | 0.000777 | 0.000043 | 0.000066 | 0.000122 | 0.000155 | 0.000116 | 0.000123 | 0.000099 | 0.000445 |
Nucleus | 0.019302 | 0.032170 | 0.014273 | 0.081717 | 0.006457 | 0.013093 | 0.026105 | 0.017186 | 0.008449 | 0.017696 | 0.020142 | 0.047288 |
Peroxisomes | 0.000016 | 0.000024 | 0.000010 | 0.000210 | 0.000004 | 0.000008 | 0.000008 | 0.000054 | 0.000006 | 0.000012 | 0.000009 | 0.000126 |
Vacuole | 0.103061 | 0.092596 | 0.123197 | 0.263998* | 0.046688 | 0.047347 | 0.126847 | 0.184143 | 0.055431 | 0.058265 | 0.125007 | 0.221393* |
Vacuole Periphery | 0.134810* | 0.035557 | 0.078590 | 0.033330 | 0.089998 | 0.040484 | 0.026190 | 0.020375 | 0.096948 | 0.039295 | 0.052599 | 0.026418 |
R1 | R2 | R1 & R2 | |||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
UBP2_WT | UBP14_WT | UBP2UBP14_WT | UBP2UBP14_UBP2 | UBP2UBP14_UBP14 | UBP2_WT | UBP14_WT | UBP2UBP14_WT | UBP2UBP14_UBP2 | UBP2UBP14_UBP14 | UBP2_WT | UBP14_WT | UBP2UBP14_WT | UBP2UBP14_UBP2 | UBP2UBP14_UBP14 | |
Actin | 0.02 | 2.77 | -4.16 | -3.77 | -5.46 | -3.02 | -8.41 | -7.16 | -6.52 | -3.83 | -2.03 | -7.29 | -8.18 | -7.22 | -6.60 |
Bud Neck | 0.54 | 0.19 | -12.93 | -13.28 | -13.23 | -2.58 | -8.47 | -5.12 | -4.39 | -1.60 | -2.79 | -9.38 | -13.74 | -12.76 | -10.63 |
Bud Site | 1.12 | 3.70 | -2.59 | -3.59 | -5.91 | -3.43 | -3.90 | -7.06 | -6.03 | -5.48 | -2.13 | -1.32 | -8.66 | -7.54 | -8.07 |
Cell Periphery | 4.79 | 4.19 | 10.14 | 8.10 | 14.13 | 2.63 | -4.06 | -13.16 | -13.96 | -11.60 | 3.30 | -5.05 | -6.89 | -9.39 | -3.13 |
Cytoplasm | -9.89 | -8.16 | 2.58 | 18.42 | 17.87 | -10.32 | 1.30 | 7.73 | 16.44 | 6.49 | -12.14 | 0.21 | 15.07 | 28.52 | 16.22 |
Cytoplasmic Foci | 3.51 | 6.97 | 0.21 | -3.00 | -6.05 | 4.04 | 6.04 | 0.32 | -1.57 | -2.69 | 4.67 | 9.45 | -1.66 | -4.16 | -6.39 |
Eisosomes | 1.26 | 3.23 | -3.30 | -4.28 | -6.13 | -2.03 | -8.38 | -9.71 | -8.33 | -4.49 | -0.79 | -4.39 | -8.39 | -7.76 | -7.14 |
Endoplasmic Reticulum | 2.62 | 1.63 | 3.11 | 1.47 | 5.16 | 6.16 | 6.33 | 5.00 | -1.87 | -2.01 | 6.60 | 5.31 | 6.09 | -1.00 | 2.49 |
Endosome | 3.67 | 4.87 | -1.43 | -2.39 | -2.65 | 4.24 | 5.04 | -0.69 | -1.47 | -1.65 | 5.37 | 7.66 | -2.12 | -2.83 | -3.06 |
Golgi | 11.17 | 11.19 | 14.57 | 10.55 | 20.74* | 13.80 | 19.98 | 18.91 | 7.57 | -0.37 | 17.15 | 19.53 | 24.85 | 13.38 | 13.95 |
Lipid Particles | 3.25 | 5.01 | 5.62 | 3.46 | 2.39 | 2.57 | -2.36 | -9.51 | -10.37 | -8.40 | 2.77 | 0.11 | -6.15 | -8.32 | -6.78 |
Mitochondria | -0.80 | 0.84 | -3.45 | -3.20 | -3.69 | -0.66 | -1.68 | -1.68 | -1.32 | -0.47 | -1.46 | -1.50 | -3.92 | -3.65 | -3.71 |
Mitotic Spindle | 2.89 | 2.66 | -5.34 | -6.20 | -6.14 | 1.29 | 3.65 | -1.23 | -1.99 | -3.09 | 1.99 | 3.93 | -5.03 | -6.10 | -6.83 |
None | -2.51 | 7.06 | -1.19 | 1.35 | -4.01 | -2.26 | -0.39 | -0.55 | 1.09 | -0.37 | -3.65 | 2.61 | -2.59 | 0.71 | -3.23 |
Nuclear Periphery | 2.73 | -0.68 | -4.24 | -6.35 | -3.93 | 5.35 | -0.07 | 2.72 | -2.61 | 2.47 | 5.47 | -2.30 | -4.21 | -8.17 | -2.22 |
Nuclear Periphery Foci | 4.15 | 4.58 | 4.59 | 0.65 | 0.57 | 8.64 | 10.00 | 8.08 | 0.16 | -1.04 | 9.43 | 10.23 | 9.10 | 0.03 | -0.16 |
Nucleolus | 0.73 | 1.57 | -2.61 | -5.76 | -8.62 | -5.61 | -9.88 | -3.87 | -3.48 | -2.31 | -0.20 | -0.08 | -7.57 | -8.08 | -8.55 |
Nucleus | -3.55 | 1.19 | -17.63 | -15.30 | -19.72 | -8.02 | -14.54 | -10.34 | -5.64 | 4.37 | -9.31 | -13.82 | -21.43 | -17.59 | -14.90 |
Peroxisomes | -1.23 | 1.70 | -3.79 | -3.50 | -3.95 | -3.85 | -2.91 | -4.70 | -4.30 | -4.19 | -3.52 | -3.02 | -5.42 | -5.02 | -5.18 |
Vacuole | 0.78 | -2.72 | -13.94 | -16.08 | -14.47 | -0.86 | -17.87 | -20.87 | -20.04 | -5.59 | -1.51 | -19.91 | -29.65 | -28.18 | -14.24 |
Vacuole Periphery | 9.53 | 5.35 | 11.45 | 4.42 | 17.38* | 12.97 | 18.61 | 20.85 | 8.86 | 3.59 | 16.09 | 12.58 | 23.81 | 9.89 | 16.70 |
Temp | Actin Patch (Sac6-tdTomato) | Cortical Patch (Sla1-GFP) | Late Endosome (Snf7-GFP) | Vacuole (Vph1-GFP) |
---|---|---|---|---|
37℃ | ||||
RT |
Gene / Allele | Actin Patch (Sac6-tdTomato) | Cortical Patch (Sla1-GFP) | Late Endosome (Snf7-GFP) | Vacuole (Sac6-tdTomato) |
---|
Gene | Images |
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Gene | Images |
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