Standard name | |
---|---|
Human Ortholog | |
Description | Protein that localizes to the endoplasmic reticulum; also associates with the nuclear pore complex; deletion extends chronological lifespan; highly conserved across species, orthologous to human TMEM33 and paralogous to Pom33p; protein abundance increases in response to DNA replication stress |
ensLOC | DeepLoc | |||||||||||||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Localization | WT1 | WT2 | WT3 | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 | WT1 | WT2 | WT3 | AF100 | AF140 | AF180 |
Cortical Patches | 17 | 18 | 10 | 7 | 7 | 14 | 16 | 11 | 12 | 17 | 15 | 19 | 15 | 20 | 15 | 10 | 18 | 3 | 5 | 4 | 2 | 4 | 6 | 7 |
Bud | 3 | 10 | 0 | 1 | 0 | 0 | 1 | 1 | 3 | 2 | 9 | 4 | 0 | 3 | 0 | 2 | 0 | 0 | 1 | 2 | 0 | 2 | 1 | 1 |
Bud Neck | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 1 | 0 | 1 | 0 | 0 |
Bud Site | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | – | – | – | – | – | – |
Cell Periphery | 18 | 9 | 13 | 10 | 3 | 30 | 14 | 17 | 6 | 10 | 15 | 12 | 50 | 96 | 96 | 43 | 42 | 11 | 2 | 0 | 3 | 5 | 10 | 16 |
Cytoplasm | 36 | 31 | 4 | 8 | 7 | 27 | 21 | 35 | 33 | 41 | 37 | 26 | 24 | 42 | 74 | 15 | 23 | 11 | 7 | 19 | 2 | 11 | 7 | 13 |
Endoplasmic Reticulum | 301 | 166 | 136 | 70 | 48 | 168 | 103 | 203 | 124 | 125 | 114 | 92 | 232 | 365 | 429 | 0 | 2 | 1 | 342 | 187 | 146 | 236 | 285 | 318 |
Endosome | 0 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 2 | 0 | 0 | 0 | 1 | 0 | 1 | 0 | 0 | 0 | 2 | 0 | 1 | 0 | 2 |
Golgi | 8 | 2 | 7 | 6 | 0 | 0 | 1 | 5 | 0 | 3 | 1 | 4 | 20 | 12 | 14 | 10 | 45 | 10 | 1 | 2 | 0 | 2 | 4 | 7 |
Mitochondria | 1 | 7 | 0 | 3 | 4 | 2 | 4 | 3 | 21 | 7 | 16 | 21 | 0 | 0 | 0 | 8 | 14 | 0 | 1 | 22 | 0 | 16 | 16 | 13 |
Nucleus | 0 | 0 | 1 | 0 | 0 | 2 | 1 | 0 | 1 | 2 | 1 | 0 | 1 | 1 | 2 | 0 | 0 | 1 | 0 | 1 | 0 | 0 | 0 | 0 |
Nuclear Periphery | 0 | 0 | 1 | 1 | 0 | 1 | 0 | 4 | 5 | 4 | 2 | 6 | 0 | 1 | 2 | 1 | 0 | 0 | 2 | 7 | 0 | 1 | 1 | 2 |
Nucleolus | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 0 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Peroxisomes | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1 |
SpindlePole | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Vac/Vac Membrane | 34 | 27 | 6 | 10 | 7 | 4 | 14 | 35 | 6 | 15 | 12 | 17 | 7 | 19 | 24 | 7 | 22 | 6 | 4 | 69 | 1 | 11 | 5 | 10 |
Unique Cell Count | 353 | 236 | 148 | 92 | 68 | 205 | 142 | 271 | 180 | 188 | 187 | 163 | 281 | 452 | 537 | 88 | 133 | 38 | 374 | 324 | 159 | 299 | 342 | 401 |
Labelled Cell Count | 419 | 271 | 178 | 116 | 76 | 248 | 175 | 314 | 211 | 228 | 222 | 202 | 349 | 561 | 656 | 97 | 166 | 43 | 374 | 324 | 159 | 299 | 342 | 401 |
Screen | WT1 | WT2 | WT3 | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 | AF100 | AF140 | AF180 |
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Mean Cell GFP Intensity (1e-4) | 22.8 | 18.7 | 17.2 | 15.9 | 13.1 | 16.0 | 15.1 | 15.1 | 12.6 | 13.6 | 13.6 | 13.6 | 20.3 | 20.5 | 20.5 | 14.1 | 17.6 | 17.0 | 19.2 | 22.6 | 21.4 |
Std Deviation (1e-4) | 4.2 | 4.6 | 2.9 | 3.1 | 3.6 | 3.3 | 3.6 | 3.2 | 3.1 | 3.3 | 3.3 | 3.2 | 3.6 | 4.2 | 4.2 | 3.2 | 5.1 | 4.2 | 4.5 | 4.7 | 4.6 |
Intensity Change (Log2) | – | – | – | -0.11 | -0.39 | -0.11 | -0.19 | -0.19 | -0.45 | -0.34 | -0.34 | -0.34 | 0.24 | 0.25 | 0.25 | -0.29 | 0.04 | -0.02 | 0.16 | 0.39 | 0.31 |
Localization | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 |
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Cortical Patches | 0.3 | 0.9 | 0 | 1.3 | -1.2 | 0 | 0.8 | 0.4 | 1.5 | -0.6 | -1.1 | -2.3 | 1.2 | 1.9 | 0 |
Bud | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Bud Neck | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Bud Site | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Cell Periphery | 0.5 | -1.1 | 1.7 | 0.3 | -1.0 | -2.1 | -1.2 | -0.3 | -0.5 | 2.5 | 3.4 | 2.7 | 7.0 | 4.8 | 0 |
Cytoplasm | 0 | 0 | 3.4 | 3.7 | 3.4 | 4.5 | 5.1 | 4.7 | 4.0 | 2.3 | 2.6 | 3.8 | 3.9 | 4.1 | 0 |
Endoplasmic Reticulum | -3.4 | -4.1 | -2.7 | -4.3 | -4.2 | -5.1 | -5.6 | -6.5 | -7.1 | -2.6 | -3.2 | -3.4 | -13.8 | -15.1 | -11.1 |
Endosome | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Golgi | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | -1.1 | 1.0 | 0 | 0 | 1.9 | 6.3 | 0 |
Mitochondria | 0 | 0 | 0 | 0 | 0 | 4.3 | 0 | 3.6 | 4.5 | 0 | 0 | 0 | 0 | 4.0 | 0 |
Nucleus | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Nuclear Periphery | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Nucleolus | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Peroxisomes | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
SpindlePole | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Vacuole | 2.1 | 0 | 0 | 2.0 | 2.9 | -0.3 | 1.5 | 1.0 | 2.1 | 0 | 0.1 | 0.2 | 0 | 3.5 | 0 |
R1 | R2 | R3 | ||||||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | |
Concentration | 12.4732 | 11.9811 | 11.5345 | 12.5082 | 11.5854 | 11.9631 | 19.751 | 19.9131 | 18.8646 | 19.4809 | 16.7591 | 19.0755 | 14.3841 | 13.783 | 14.3403 | 14.4937 | 14.2439 | 13.9981 |
Actin | 0.0388 | 0.02 | 0.0149 | 0.002 | 0.0154 | 0.016 | 0.0079 | 0.0001 | 0.0085 | 0 | 0.0012 | 0.0007 | 0.0121 | 0.0048 | 0.0106 | 0.1284 | 0.0001 | 0.0157 |
Bud | 0.0006 | 0.0003 | 0.0009 | 0.0001 | 0.0051 | 0.0001 | 0.0001 | 0.0001 | 0.0002 | 0 | 0 | 0 | 0.0003 | 0.0001 | 0.0002 | 0.0006 | 0 | 0.0006 |
Bud Neck | 0.0022 | 0.0018 | 0.0007 | 0.0016 | 0.0076 | 0.0032 | 0.0002 | 0.0002 | 0.0002 | 0 | 0.0001 | 0.0011 | 0.0009 | 0.0011 | 0.0011 | 0.0032 | 0.0003 | 0.0041 |
Bud Periphery | 0.0016 | 0.0008 | 0.0062 | 0.0008 | 0.0048 | 0.0006 | 0.0004 | 0.0002 | 0.001 | 0 | 0.0002 | 0.0002 | 0.0009 | 0.0003 | 0.0008 | 0.0013 | 0.0001 | 0.0014 |
Bud Site | 0.0022 | 0.0116 | 0.0004 | 0.0001 | 0.0053 | 0.0002 | 0.0005 | 0.0084 | 0.0002 | 0 | 0.0001 | 0 | 0.0015 | 0.0061 | 0.0018 | 0.0011 | 0 | 0.0045 |
Cell Periphery | 0.0078 | 0.0296 | 0.0113 | 0.0141 | 0.0077 | 0.0079 | 0.0078 | 0.0087 | 0.0053 | 0.0028 | 0.0053 | 0.0075 | 0.0097 | 0.0157 | 0.0059 | 0.0046 | 0.0067 | 0.0058 |
Cytoplasm | 0.0233 | 0.0272 | 0.0162 | 0.0143 | 0.0168 | 0.009 | 0.0113 | 0.0075 | 0.0049 | 0.0007 | 0.0048 | 0.0045 | 0.0406 | 0.0337 | 0.0245 | 0.0026 | 0.004 | 0.0138 |
Cytoplasmic Foci | 0.0072 | 0.0013 | 0.0079 | 0.0006 | 0.0033 | 0.0012 | 0.0006 | 0.0002 | 0.0027 | 0.0001 | 0.0004 | 0.0003 | 0.0046 | 0.0056 | 0.0029 | 0.0115 | 0.0002 | 0.0032 |
Eisosomes | 0.0007 | 0.0029 | 0.0003 | 0.0003 | 0.0295 | 0.004 | 0.0011 | 0 | 0.0004 | 0.0001 | 0.0003 | 0.0001 | 0.0004 | 0.001 | 0.0006 | 0.002 | 0.0001 | 0.0002 |
Endoplasmic Reticulum | 0.8037 | 0.8554 | 0.857 | 0.9388 | 0.8122 | 0.9194 | 0.9358 | 0.9588 | 0.9405 | 0.9911 | 0.9717 | 0.9762 | 0.8574 | 0.8695 | 0.8978 | 0.6532 | 0.9656 | 0.9139 |
Endosome | 0.0167 | 0.004 | 0.0088 | 0.0026 | 0.0081 | 0.0049 | 0.0057 | 0.0007 | 0.0048 | 0.0006 | 0.0021 | 0.0012 | 0.0172 | 0.009 | 0.0093 | 0.0163 | 0.0011 | 0.0068 |
Golgi | 0.0073 | 0.0141 | 0.0062 | 0.0042 | 0.0128 | 0.0109 | 0.0065 | 0.0003 | 0.007 | 0.0012 | 0.0015 | 0.0021 | 0.0112 | 0.0189 | 0.0109 | 0.032 | 0.001 | 0.006 |
Lipid Particles | 0.0088 | 0.0101 | 0.0099 | 0.003 | 0.0178 | 0.0051 | 0.0057 | 0.0038 | 0.0083 | 0.0011 | 0.0057 | 0.0026 | 0.014 | 0.0224 | 0.006 | 0.0742 | 0.0151 | 0.0092 |
Mitochondria | 0.0127 | 0.0002 | 0.0159 | 0.0002 | 0.0056 | 0.0008 | 0.0035 | 0 | 0.0056 | 0 | 0.0005 | 0.0001 | 0.0046 | 0.0003 | 0.0158 | 0.0327 | 0 | 0.003 |
None | 0.0071 | 0.0015 | 0.0049 | 0.0002 | 0.002 | 0.0004 | 0.0006 | 0.0002 | 0.0001 | 0 | 0.0007 | 0.0001 | 0.006 | 0.0005 | 0.0021 | 0.0007 | 0 | 0.0003 |
Nuclear Periphery | 0.0403 | 0.0141 | 0.0148 | 0.0124 | 0.0155 | 0.0124 | 0.0081 | 0.0035 | 0.0026 | 0.0016 | 0.0032 | 0.0016 | 0.01 | 0.0069 | 0.005 | 0.0044 | 0.0026 | 0.0049 |
Nucleolus | 0.0005 | 0.0001 | 0.0003 | 0 | 0.0016 | 0.0001 | 0.0001 | 0.0004 | 0 | 0 | 0 | 0 | 0.0001 | 0.0001 | 0 | 0.0001 | 0 | 0.0004 |
Nucleus | 0.0041 | 0.0008 | 0.0022 | 0.0004 | 0.0203 | 0.0009 | 0.0007 | 0.0012 | 0.0001 | 0 | 0.0001 | 0.0001 | 0.0007 | 0.0005 | 0.0004 | 0.0004 | 0.0001 | 0.0011 |
Peroxisomes | 0.0013 | 0.0001 | 0.0009 | 0 | 0.0006 | 0.0001 | 0.0001 | 0 | 0.0046 | 0 | 0 | 0 | 0.0004 | 0.0001 | 0.0003 | 0.0236 | 0 | 0.0014 |
Punctate Nuclear | 0.0057 | 0.0003 | 0.0138 | 0.0001 | 0.0025 | 0.0003 | 0.0002 | 0.0002 | 0 | 0 | 0 | 0 | 0.0003 | 0.0003 | 0.0003 | 0.0025 | 0 | 0.0004 |
Vacuole | 0.0047 | 0.0036 | 0.0052 | 0.0033 | 0.0041 | 0.0018 | 0.0026 | 0.0055 | 0.0021 | 0.0006 | 0.0015 | 0.0014 | 0.0062 | 0.003 | 0.0031 | 0.0033 | 0.0029 | 0.003 |
Vacuole Periphery | 0.0025 | 0.0004 | 0.0012 | 0.0011 | 0.0011 | 0.0006 | 0.0006 | 0.0001 | 0.0007 | 0.0001 | 0.0003 | 0.0001 | 0.0008 | 0.0002 | 0.0005 | 0.0013 | 0.0001 | 0.0005 |
R1 | R2 | |||||||||
---|---|---|---|---|---|---|---|---|---|---|
G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | |
Gene Expression | 40.9414 | 16.7996 | 26.7129 | 40.6414 | 29.5869 | 38.9063 | 38.2186 | 40.1255 | 30.1354 | 44.318 |
Translational Efficiency | 0.7449 | 1.4437 | 0.9833 | 0.4885 | 0.6585 | 0.8333 | 0.9284 | 0.8407 | 1.0155 | 0.7179 |
Dataset | Hit |
---|---|
Protein Concentration | ✘ |
Protein Localization | ✘ |
Gene Expression | ✘ |
Translational Efficiency | ✘ |
R1 | R2 | R1 & R2 | |||||||||
---|---|---|---|---|---|---|---|---|---|---|---|
WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 |
122 | 874 | 2113 | 1621 | 1532 | 1983 | 772 | 1712 | 1654 | 2857 | 2885 | 3333 |
R1 | R2 | R1 & R2 | ||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|
WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 | |
Mean | 1596.44 | 1758.79 | 1568.97 | 1681.94 | 1397.38 | 1735.98 | 1721.53 | 1539.62 | 1412.06 | 1742.96 | 1609.79 | 1608.84 |
Standard Deviation | 359.25 | 303.37 | 242.59 | 471.55 | 244.22 | 298.87 | 558.61 | 305.90 | 259.75 | 300.44 | 362.17 | 401.59 |
Intensity Change Log 2 | — | 0.139725 | -0.025041 | 0.075268 | — | 0.313026 | 0.300967 | 0.139850 | — | 0.223210 | 0.136319 | 0.105772 |
R1 | R2 | R1 & R2 | ||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|
WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 | |
Actin | 0.000580 | 0.000945 | 0.003185 | 0.004191 | 0.000745 | 0.001040 | 0.002452 | 0.011749 | 0.000733 | 0.001011 | 0.002989 | 0.008073 |
Bud Neck | 0.001035 | 0.000437 | 0.002386 | 0.003316 | 0.000426 | 0.000310 | 0.006117 | 0.007422 | 0.000471 | 0.000349 | 0.003384 | 0.005425 |
Bud Site | 0.010475 | 0.002155 | 0.010613 | 0.019985 | 0.003488 | 0.002441 | 0.015770 | 0.051951 | 0.004003 | 0.002354 | 0.011993 | 0.036404 |
Cell Periphery | 0.029526 | 0.010339 | 0.020126 | 0.146555* | 0.010977 | 0.009692 | 0.046251 | 0.080075 | 0.012346 | 0.009890 | 0.027117 | 0.112407* |
Cytoplasm | 0.002301 | 0.000381 | 0.007371 | 0.008084 | 0.002029 | 0.002570 | 0.003356 | 0.036488 | 0.002049 | 0.001900 | 0.006296 | 0.022674 |
Cytoplasmic Foci | 0.008370 | 0.001875 | 0.000863 | 0.003086 | 0.001754 | 0.000925 | 0.000783 | 0.008256 | 0.002242 | 0.001216 | 0.000841 | 0.005742 |
Eisosomes | 0.000185 | 0.000252 | 0.000710 | 0.001724 | 0.000401 | 0.000283 | 0.000570 | 0.001551 | 0.000385 | 0.000273 | 0.000673 | 0.001635 |
Endoplasmic Reticulum | 0.887454* | 0.963801* | 0.921861* | 0.684063* | 0.961980* | 0.963809* | 0.875804* | 0.654895* | 0.956483* | 0.963806* | 0.909536* | 0.669081* |
Endosome | 0.011530 | 0.005536 | 0.004843 | 0.008063 | 0.005861 | 0.004941 | 0.005938 | 0.011077 | 0.006280 | 0.005123 | 0.005136 | 0.009611 |
Golgi | 0.011224 | 0.005253 | 0.001878 | 0.008396 | 0.002600 | 0.001734 | 0.002383 | 0.008018 | 0.003237 | 0.002810 | 0.002014 | 0.008202 |
Lipid Particles | 0.010464 | 0.004228 | 0.015505 | 0.071118 | 0.004860 | 0.005816 | 0.021534 | 0.099889 | 0.005273 | 0.005330 | 0.017118 | 0.085896 |
Mitochondria | 0.002479 | 0.000335 | 0.000647 | 0.003184 | 0.000396 | 0.000863 | 0.000329 | 0.001712 | 0.000550 | 0.000701 | 0.000562 | 0.002428 |
Mitotic Spindle | 0.003537 | 0.000175 | 0.000161 | 0.001486 | 0.000089 | 0.000150 | 0.000819 | 0.001596 | 0.000344 | 0.000158 | 0.000337 | 0.001542 |
None | 0.000205 | 0.000157 | 0.000852 | 0.001057 | 0.000908 | 0.000728 | 0.000183 | 0.000655 | 0.000856 | 0.000554 | 0.000673 | 0.000850 |
Nuclear Periphery | 0.001036 | 0.000432 | 0.000536 | 0.007505 | 0.000310 | 0.000390 | 0.001350 | 0.001351 | 0.000364 | 0.000403 | 0.000754 | 0.004344 |
Nuclear Periphery Foci | 0.003828 | 0.001141 | 0.002317 | 0.001784 | 0.001074 | 0.000840 | 0.002791 | 0.003157 | 0.001277 | 0.000932 | 0.002444 | 0.002489 |
Nucleolus | 0.002138 | 0.000003 | 0.000009 | 0.000064 | 0.000005 | 0.000007 | 0.000020 | 0.000040 | 0.000163 | 0.000005 | 0.000012 | 0.000051 |
Nucleus | 0.003824 | 0.000030 | 0.000324 | 0.000968 | 0.000073 | 0.000090 | 0.001323 | 0.001506 | 0.000350 | 0.000071 | 0.000591 | 0.001244 |
Peroxisomes | 0.000438 | 0.000152 | 0.001046 | 0.008095 | 0.000505 | 0.001459 | 0.000496 | 0.003846 | 0.000500 | 0.001059 | 0.000899 | 0.005913 |
Vacuole | 0.005885 | 0.001640 | 0.004380 | 0.014531 | 0.000932 | 0.001391 | 0.010662 | 0.013206 | 0.001297 | 0.001467 | 0.006061 | 0.013851 |
Vacuole Periphery | 0.003488 | 0.000731 | 0.000387 | 0.002744 | 0.000584 | 0.000522 | 0.001069 | 0.001559 | 0.000798 | 0.000586 | 0.000569 | 0.002136 |
R1 | R2 | R1 & R2 | |||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
UBP2_WT | UBP14_WT | UBP2UBP14_WT | UBP2UBP14_UBP2 | UBP2UBP14_UBP14 | UBP2_WT | UBP14_WT | UBP2UBP14_WT | UBP2UBP14_UBP2 | UBP2UBP14_UBP14 | UBP2_WT | UBP14_WT | UBP2UBP14_WT | UBP2UBP14_UBP2 | UBP2UBP14_UBP14 | |
Actin | -0.77 | -4.76 | -5.14 | -4.20 | -1.21 | -0.72 | -1.96 | -10.06 | -9.38 | -6.75 | -0.85 | -4.94 | -11.05 | -10.02 | -6.52 |
Bud Neck | 1.58 | -2.76 | -4.48 | -6.88 | -1.86 | 1.06 | -5.30 | -11.09 | -11.31 | -0.30 | 1.13 | -7.36 | -12.96 | -13.38 | -3.25 |
Bud Site | 1.66 | -0.05 | -1.85 | -7.88 | -3.81 | 1.17 | -4.52 | -13.93 | -14.38 | -7.78 | 1.89 | -5.83 | -14.81 | -16.24 | -10.08 |
Cell Periphery | 3.02 | 1.50 | -14.81 | -26.07* | -24.69* | 0.91 | -8.64 | -19.35 | -20.47 | -6.59 | 1.74 | -7.94 | -30.73 | -33.02* | -25.59 |
Cytoplasm | 1.49 | -3.44 | -3.80 | -7.93 | -0.90 | -0.69 | -2.95 | -13.22 | -13.01 | -9.54 | 0.22 | -6.08 | -13.79 | -14.26 | -9.78 |
Cytoplasmic Foci | 1.30 | 1.51 | 1.06 | -1.70 | -4.87 | 2.11 | 1.29 | -5.54 | -6.40 | -6.10 | 1.90 | 2.49 | -4.58 | -6.93 | -7.49 |
Eisosomes | -1.13 | -2.11 | -12.28 | -11.32 | -3.69 | 0.68 | -0.92 | -5.18 | -8.02 | -6.00 | 0.70 | -1.21 | -6.91 | -13.04 | -4.69 |
Endoplasmic Reticulum | -3.77 | -1.70 | 9.46 | 32.98* | 28.22 | -0.51 | 10.44 | 35.85* | 36.41* | 17.23 | -2.13 | 10.93 | 45.92 | 49.79* | 35.52* |
Endosome | 1.80 | 2.03 | 1.07 | -3.02 | -6.50 | 1.72 | -0.27 | -7.57 | -9.43 | -6.01 | 2.07 | 1.99 | -5.98 | -9.61 | -9.80 |
Golgi | 2.31 | 3.72 | 1.07 | -2.61 | -6.34 | 1.90 | 0.44 | -5.31 | -6.69 | -5.87 | 0.83 | 2.19 | -6.31 | -7.67 | -8.38 |
Lipid Particles | 1.95 | -1.40 | -12.54 | -17.99 | -14.24 | -1.12 | -5.92 | -22.11 | -21.79 | -15.18 | -0.06 | -8.42 | -28.07 | -28.08 | -22.04 |
Mitochondria | 1.40 | 1.19 | -0.49 | -5.30 | -4.75 | -1.94 | 0.35 | -5.75 | -2.64 | -5.87 | -0.73 | -0.20 | -6.12 | -5.32 | -6.39 |
Mitotic Spindle | 1.00 | 1.01 | 0.61 | -7.30 | -8.94 | -0.75 | -1.99 | -3.31 | -3.15 | -1.28 | 0.73 | -0.06 | -3.50 | -5.39 | -4.38 |
None | 0.40 | -2.67 | -3.23 | -3.60 | -0.70 | 0.47 | 2.00 | 0.63 | 0.26 | -2.84 | 0.89 | 0.49 | -0.05 | -1.73 | -0.89 |
Nuclear Periphery | 2.07 | 1.73 | -11.37 | -14.34 | -14.18 | -1.58 | -6.96 | -11.74 | -10.35 | 0.19 | -0.83 | -6.83 | -15.88 | -15.75 | -14.18 |
Nuclear Periphery Foci | 1.05 | 0.64 | 0.85 | -0.90 | 1.83 | 0.75 | -3.18 | -6.10 | -6.99 | -0.27 | 0.92 | -3.19 | -3.81 | -5.41 | 0.08 |
Nucleolus | 1.01 | 1.01 | 0.99 | -10.27 | -9.11 | -0.78 | -3.81 | -9.12 | -8.59 | -2.27 | 1.01 | 0.96 | 0.72 | -13.00 | -9.98 |
Nucleus | 1.06 | 0.97 | 0.74 | -4.06 | -2.99 | -1.17 | -3.27 | -7.47 | -7.38 | 1.75 | 1.05 | -2.03 | -3.16 | -7.54 | -0.77 |
Peroxisomes | 1.44 | -2.23 | -8.91 | -9.53 | -8.30 | -3.22 | 0.06 | -8.69 | -5.02 | -7.52 | -2.64 | -2.55 | -11.86 | -9.84 | -10.64 |
Vacuole | 1.94 | 0.69 | -3.63 | -16.07 | -11.26 | -1.75 | -7.57 | -14.84 | -13.72 | -0.19 | -0.60 | -8.88 | -21.60 | -21.09 | -8.99 |
Vacuole Periphery | 2.37 | 2.68 | 0.62 | -6.19 | -7.70 | 0.34 | -1.35 | -3.65 | -3.50 | -1.05 | 1.33 | 1.34 | -6.02 | -6.94 | -6.81 |
Temp | Actin Patch (Sac6-tdTomato) | Cortical Patch (Sla1-GFP) | Late Endosome (Snf7-GFP) | Vacuole (Vph1-GFP) |
---|---|---|---|---|
37℃ | ||||
RT |
Gene / Allele | Actin Patch (Sac6-tdTomato) | Cortical Patch (Sla1-GFP) | Late Endosome (Snf7-GFP) | Vacuole (Sac6-tdTomato) |
---|
Gene | Images |
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Gene | Images |
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