Standard name
Human Ortholog
Description Protein that localizes to the endoplasmic reticulum; also associates with the nuclear pore complex; deletion extends chronological lifespan; highly conserved across species, orthologous to human TMEM33 and paralogous to Pom33p; protein abundance increases in response to DNA replication stress
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Micrographs

ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0.05 0.08 0.07 0.08 0.1 0.07 0.11 0 0.07 0.09 0.08 0.12 0.05 0 0 0.11 0.14 0.08 0 0 0 0 0 0
Bud 0 0 0 0 0 0 0 0 0 0 0.05 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 – – – – – –
Cell Periphery 0.05 0 0.09 0.11 0 0.15 0.1 0.06 0 0.05 0.08 0.07 0.18 0.21 0.18 0.49 0.32 0.29 0 0 0 0 0 0
Cytoplasm 0.1 0.13 0 0.09 0.1 0.13 0.15 0.13 0.18 0.22 0.2 0.16 0.09 0.09 0.14 0.17 0.17 0.29 0 0.06 0 0 0 0
Endoplasmic Reticulum 0.85 0.7 0.92 0.76 0.71 0.82 0.73 0.75 0.69 0.66 0.61 0.56 0.83 0.81 0.8 0 0 0 0.92 0.58 0.92 0.79 0.84 0.79
Endosome 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Golgi 0 0 0.05 0.07 0 0 0 0 0 0 0 0 0.07 0 0 0.11 0.34 0.26 0 0 0 0 0 0
Mitochondria 0 0 0 0 0.06 0 0 0 0.12 0 0.09 0.13 0 0 0 0.09 0.11 0 0 0.07 0 0.06 0.05 0
Nucleus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nuclear Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Vac/Vac Membrane 0.1 0.11 0 0.11 0.1 0 0.1 0.13 0 0.08 0.06 0.1 0 0 0 0.08 0.17 0.16 0 0.22 0 0 0 0
ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 17 18 10 7 7 14 16 11 12 17 15 19 15 20 15 10 18 3 5 4 2 4 6 7
Bud 3 10 0 1 0 0 1 1 3 2 9 4 0 3 0 2 0 0 1 2 0 2 1 1
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 1 1 0 1 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 – – – – – –
Cell Periphery 18 9 13 10 3 30 14 17 6 10 15 12 50 96 96 43 42 11 2 0 3 5 10 16
Cytoplasm 36 31 4 8 7 27 21 35 33 41 37 26 24 42 74 15 23 11 7 19 2 11 7 13
Endoplasmic Reticulum 301 166 136 70 48 168 103 203 124 125 114 92 232 365 429 0 2 1 342 187 146 236 285 318
Endosome 0 1 0 0 0 0 0 0 0 2 0 0 0 1 0 1 0 0 0 2 0 1 0 2
Golgi 8 2 7 6 0 0 1 5 0 3 1 4 20 12 14 10 45 10 1 2 0 2 4 7
Mitochondria 1 7 0 3 4 2 4 3 21 7 16 21 0 0 0 8 14 0 1 22 0 16 16 13
Nucleus 0 0 1 0 0 2 1 0 1 2 1 0 1 1 2 0 0 1 0 1 0 0 0 0
Nuclear Periphery 0 0 1 1 0 1 0 4 5 4 2 6 0 1 2 1 0 0 2 7 0 1 1 2
Nucleolus 1 0 0 0 0 0 0 0 0 0 0 1 0 1 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 1
SpindlePole 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Vac/Vac Membrane 34 27 6 10 7 4 14 35 6 15 12 17 7 19 24 7 22 6 4 69 1 11 5 10
Unique Cell Count 353 236 148 92 68 205 142 271 180 188 187 163 281 452 537 88 133 38 374 324 159 299 342 401
Labelled Cell Count 419 271 178 116 76 248 175 314 211 228 222 202 349 561 656 97 166 43 374 324 159 299 342 401


Endoplasmic Reticulum

(Huh et al. Nature 2003 425:686)
Screen WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 AF100 AF140 AF180
Mean Cell GFP Intensity (1e-4) 22.8 18.7 17.2 15.9 13.1 16.0 15.1 15.1 12.6 13.6 13.6 13.6 20.3 20.5 20.5 14.1 17.6 17.0 19.2 22.6 21.4
Std Deviation (1e-4) 4.2 4.6 2.9 3.1 3.6 3.3 3.6 3.2 3.1 3.3 3.3 3.2 3.6 4.2 4.2 3.2 5.1 4.2 4.5 4.7 4.6
Intensity Change (Log2) – – – -0.11 -0.39 -0.11 -0.19 -0.19 -0.45 -0.34 -0.34 -0.34 0.24 0.25 0.25 -0.29 0.04 -0.02 0.16 0.39 0.31


Localization RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3
Cortical Patches 0.3 0.9 0 1.3 -1.2 0 0.8 0.4 1.5 -0.6 -1.1 -2.3 1.2 1.9 0
Bud 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cell Periphery 0.5 -1.1 1.7 0.3 -1.0 -2.1 -1.2 -0.3 -0.5 2.5 3.4 2.7 7.0 4.8 0
Cytoplasm 0 0 3.4 3.7 3.4 4.5 5.1 4.7 4.0 2.3 2.6 3.8 3.9 4.1 0
Endoplasmic Reticulum -3.4 -4.1 -2.7 -4.3 -4.2 -5.1 -5.6 -6.5 -7.1 -2.6 -3.2 -3.4 -13.8 -15.1 -11.1
Endosome 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Golgi 0 0 0 0 0 0 0 0 -1.1 1.0 0 0 1.9 6.3 0
Mitochondria 0 0 0 0 0 4.3 0 3.6 4.5 0 0 0 0 4.0 0
Nucleus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nuclear Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Vacuole 2.1 0 0 2.0 2.9 -0.3 1.5 1.0 2.1 0 0.1 0.2 0 3.5 0

Images

Circle plot not available

Protein Concentration and Protein Localization Data

R1 R2 R3
G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase
Concentration 12.4732 11.9811 11.5345 12.5082 11.5854 11.9631 19.751 19.9131 18.8646 19.4809 16.7591 19.0755 14.3841 13.783 14.3403 14.4937 14.2439 13.9981
Actin 0.0388 0.02 0.0149 0.002 0.0154 0.016 0.0079 0.0001 0.0085 0 0.0012 0.0007 0.0121 0.0048 0.0106 0.1284 0.0001 0.0157
Bud 0.0006 0.0003 0.0009 0.0001 0.0051 0.0001 0.0001 0.0001 0.0002 0 0 0 0.0003 0.0001 0.0002 0.0006 0 0.0006
Bud Neck 0.0022 0.0018 0.0007 0.0016 0.0076 0.0032 0.0002 0.0002 0.0002 0 0.0001 0.0011 0.0009 0.0011 0.0011 0.0032 0.0003 0.0041
Bud Periphery 0.0016 0.0008 0.0062 0.0008 0.0048 0.0006 0.0004 0.0002 0.001 0 0.0002 0.0002 0.0009 0.0003 0.0008 0.0013 0.0001 0.0014
Bud Site 0.0022 0.0116 0.0004 0.0001 0.0053 0.0002 0.0005 0.0084 0.0002 0 0.0001 0 0.0015 0.0061 0.0018 0.0011 0 0.0045
Cell Periphery 0.0078 0.0296 0.0113 0.0141 0.0077 0.0079 0.0078 0.0087 0.0053 0.0028 0.0053 0.0075 0.0097 0.0157 0.0059 0.0046 0.0067 0.0058
Cytoplasm 0.0233 0.0272 0.0162 0.0143 0.0168 0.009 0.0113 0.0075 0.0049 0.0007 0.0048 0.0045 0.0406 0.0337 0.0245 0.0026 0.004 0.0138
Cytoplasmic Foci 0.0072 0.0013 0.0079 0.0006 0.0033 0.0012 0.0006 0.0002 0.0027 0.0001 0.0004 0.0003 0.0046 0.0056 0.0029 0.0115 0.0002 0.0032
Eisosomes 0.0007 0.0029 0.0003 0.0003 0.0295 0.004 0.0011 0 0.0004 0.0001 0.0003 0.0001 0.0004 0.001 0.0006 0.002 0.0001 0.0002
Endoplasmic Reticulum 0.8037 0.8554 0.857 0.9388 0.8122 0.9194 0.9358 0.9588 0.9405 0.9911 0.9717 0.9762 0.8574 0.8695 0.8978 0.6532 0.9656 0.9139
Endosome 0.0167 0.004 0.0088 0.0026 0.0081 0.0049 0.0057 0.0007 0.0048 0.0006 0.0021 0.0012 0.0172 0.009 0.0093 0.0163 0.0011 0.0068
Golgi 0.0073 0.0141 0.0062 0.0042 0.0128 0.0109 0.0065 0.0003 0.007 0.0012 0.0015 0.0021 0.0112 0.0189 0.0109 0.032 0.001 0.006
Lipid Particles 0.0088 0.0101 0.0099 0.003 0.0178 0.0051 0.0057 0.0038 0.0083 0.0011 0.0057 0.0026 0.014 0.0224 0.006 0.0742 0.0151 0.0092
Mitochondria 0.0127 0.0002 0.0159 0.0002 0.0056 0.0008 0.0035 0 0.0056 0 0.0005 0.0001 0.0046 0.0003 0.0158 0.0327 0 0.003
None 0.0071 0.0015 0.0049 0.0002 0.002 0.0004 0.0006 0.0002 0.0001 0 0.0007 0.0001 0.006 0.0005 0.0021 0.0007 0 0.0003
Nuclear Periphery 0.0403 0.0141 0.0148 0.0124 0.0155 0.0124 0.0081 0.0035 0.0026 0.0016 0.0032 0.0016 0.01 0.0069 0.005 0.0044 0.0026 0.0049
Nucleolus 0.0005 0.0001 0.0003 0 0.0016 0.0001 0.0001 0.0004 0 0 0 0 0.0001 0.0001 0 0.0001 0 0.0004
Nucleus 0.0041 0.0008 0.0022 0.0004 0.0203 0.0009 0.0007 0.0012 0.0001 0 0.0001 0.0001 0.0007 0.0005 0.0004 0.0004 0.0001 0.0011
Peroxisomes 0.0013 0.0001 0.0009 0 0.0006 0.0001 0.0001 0 0.0046 0 0 0 0.0004 0.0001 0.0003 0.0236 0 0.0014
Punctate Nuclear 0.0057 0.0003 0.0138 0.0001 0.0025 0.0003 0.0002 0.0002 0 0 0 0 0.0003 0.0003 0.0003 0.0025 0 0.0004
Vacuole 0.0047 0.0036 0.0052 0.0033 0.0041 0.0018 0.0026 0.0055 0.0021 0.0006 0.0015 0.0014 0.0062 0.003 0.0031 0.0033 0.0029 0.003
Vacuole Periphery 0.0025 0.0004 0.0012 0.0011 0.0011 0.0006 0.0006 0.0001 0.0007 0.0001 0.0003 0.0001 0.0008 0.0002 0.0005 0.0013 0.0001 0.0005

Sequencing Data

R1 R2
G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Post-START S/G2 Metaphase Anaphase Telophase
Gene Expression 40.9414 16.7996 26.7129 40.6414 29.5869 38.9063 38.2186 40.1255 30.1354 44.318
Translational Efficiency 0.7449 1.4437 0.9833 0.4885 0.6585 0.8333 0.9284 0.8407 1.0155 0.7179

Hit Data

Dataset Hit
Protein Concentration ✘
Protein Localization ✘
Gene Expression ✘
Translational Efficiency ✘
Standard name
Human Ortholog
Description Protein that localizes to the endoplasmic reticulum; also associates with the nuclear pore complex; deletion extends chronological lifespan; highly conserved across species, orthologous to human TMEM33 and paralogous to Pom33p; protein abundance increases in response to DNA replication stress
Localization
Cell Percentages ER (99%)
Cell Cycle Regulation No
Subcompartmental Group ER-5

Per33

Per33


Nearest neighbours ( genes)

correlation threshold

0.6

1.0

0.80


Biological Process (Gene Ontology)
Molecular Function (Gene Ontology)
Cellular Component (Gene Ontology)

Endocytosis
Temp Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Vph1-GFP)
37℃
RT
Cell Cycle Omics

CYCLoPs (Per33-GFP)
Gene / Allele Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Sac6-tdTomato)
Gene Images
Gene Images
Images are not yet available
Images are not yet available