Standard name
Human Ortholog
Description Specific translational activator for the COX1 mRNA; binds to the COX1 mRNA; also influences stability of intron-containing COX1 primary transcripts; localizes to the mitochondrial inner membrane; contains 12 pentatricopeptide repeats (PPRs)
DOWNLOAD

Micrographs

ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 0 0 0 0 0 0 0.1 0.12 0 – – 0 0 0 0 0 0 0
Bud 0 0 0 0.05 0 0 0 0.07 0.05 0.06 0.07 0.05 0 0 0 – – 0.05 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0.08 0 – – 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 – – 0 – – – – – –
Cell Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0.06 0 0.05 – – 0 0 0 0 0 0 0
Cytoplasm 0.16 0.22 0.09 0.1 0.09 0.07 0 0.05 0 0 0 0 0.52 0.67 0.79 – – 0.26 0 0 0 0 0 0
Endoplasmic Reticulum 0 0 0 0 0 0 0 0 0 0 0 0 0.07 0.05 0 – – 0.18 0 0 0 0 0 0
Endosome 0.11 0 0.07 0.08 0.18 0 0 0 0 0 0 0 0 0 0 – – 0 0.09 0.11 0.12 0.1 0.11 0.11
Golgi 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 – – 0 0 0 0 0 0 0
Mitochondria 0.56 0.77 0.7 0.71 0.7 0.89 0.89 0.87 0.94 0.95 0.95 0.94 0.14 0 0 – – 0.16 0.7 0.69 0.57 0.69 0.58 0.59
Nucleus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 – – 0 0 0 0 0 0 0
Nuclear Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 – – 0 0 0 0 0 0 0
Nucleolus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 – – 0 0 0 0 0 0 0
Peroxisomes 0.11 0 0.19 0.23 0.17 0 0 0.08 0 0 0 0 0 0 0 – – 0.13 0.05 0 0.11 0.06 0.09 0.06
SpindlePole 0.15 0 0.12 0.12 0.12 0.06 0.11 0.09 0.06 0.07 0.07 0.06 0.15 0.1 0 – – 0.26 0 0 0 0 0 0
Vac/Vac Membrane 0.07 0 0 0 0.06 0 0 0 0 0 0 0 0 0 0.05 – – 0 0 0 0 0 0 0
ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 4 7 0 1 0 0 0 0 0 0 0 0 7 13 8 – – 1 10 6 11 7 10 17
Bud 10 8 8 13 13 9 22 55 20 37 25 20 3 3 2 – – 2 0 0 0 0 3 4
Bud Neck 4 2 1 1 1 0 1 1 0 0 0 0 2 8 5 – – 1 4 1 3 3 7 11
Bud Site 4 1 1 3 1 5 11 18 7 20 5 9 0 0 0 – – 0 – – – – – –
Cell Periphery 7 2 4 5 2 3 2 2 0 5 1 1 4 1 10 – – 0 1 0 0 0 1 1
Cytoplasm 50 48 24 25 33 32 21 39 13 21 16 19 37 71 168 – – 10 8 7 2 0 3 5
Endoplasmic Reticulum 10 6 0 3 1 2 1 1 3 0 1 2 5 5 8 – – 7 3 1 2 2 2 5
Endosome 35 0 18 19 68 12 19 29 6 1 0 3 1 0 3 – – 1 38 26 37 28 35 46
Golgi 0 0 1 1 0 0 0 0 0 0 0 0 0 0 0 – – 1 2 1 8 3 9 9
Mitochondria 174 167 189 175 266 435 479 687 417 606 358 408 10 3 2 – – 6 286 166 180 189 195 240
Nucleus 0 1 1 1 2 2 10 15 5 9 3 5 0 1 0 – – 1 0 0 0 0 1 0
Nuclear Periphery 1 3 1 0 0 2 1 3 6 4 4 8 0 0 0 – – 0 2 0 4 5 2 2
Nucleolus 0 1 0 0 0 4 5 18 4 11 3 0 1 0 0 – – 1 1 0 2 0 0 4
Peroxisomes 35 0 52 56 65 21 23 60 6 16 5 7 1 0 0 – – 5 21 5 34 16 31 25
SpindlePole 48 6 33 31 44 27 60 71 27 44 26 28 11 11 9 – – 10 12 7 9 5 10 11
Vac/Vac Membrane 23 4 7 4 22 19 24 29 10 11 9 12 1 1 10 – – 1 9 9 3 6 13 16
Unique Cell Count 310 216 270 248 381 489 536 794 444 638 376 434 71 106 213 38 410 241 316 273 336 411
Labelled Cell Count 405 256 340 338 518 573 679 1028 524 785 456 522 83 117 225 47 410 241 316 273 336 411


Mitochondria, Punctate Nuclear

(Huh et al. Nature 2003 425:686)
Screen WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 AF100 AF140 AF180
Mean Cell GFP Intensity (1e-4) 4.4 3.5 4.0 3.9 4.0 3.3 3.2 3.4 3.1 2.9 3.1 3.1 5.0 5.0 5.2 3.7 4.3 5.4 5.5 5.7 5.8
Std Deviation (1e-4) 1.2 0.4 1.3 1.1 1.5 1.2 0.9 1.2 0.8 1.1 1.1 0.9 0.5 0.6 0.6 0.9 1.2 1.9 1.6 1.5 1.7
Intensity Change (Log2) – – – -0.04 -0.01 -0.25 -0.31 -0.23 -0.34 -0.43 -0.38 -0.37 0.32 0.33 0.4 -0.1 0.1 0.43 0.46 0.52 0.54


Localization RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3
Actin – – – – – – – – – – – – – – –
Bud – – – – – – – – – – – – – – –
Bud Neck – – – – – – – – – – – – – – –
Bud Site – – – – – – – – – – – – – – –
Cell Periphery – – – – – – – – – – – – – – –
Cyto – – – – – – – – – – – – – – –
Endoplasmic Reticulum – – – – – – – – – – – – – – –
Endosome – – – – – – – – – – – – – – –
Golgi – – – – – – – – – – – – – – –
Mitochondria – – – – – – – – – – – – – – –
Nuclear Periphery – – – – – – – – – – – – – – –
Nuc – – – – – – – – – – – – – – –
Nucleolus – – – – – – – – – – – – – – –
Peroxisomes – – – – – – – – – – – – – – –
SpindlePole – – – – – – – – – – – – – – –
Vac – – – – – – – – – – – – – – –
Cortical Patches – – – – – – – – – – – – – – –
Cytoplasm – – – – – – – – – – – – – – –
Nucleus – – – – – – – – – – – – – – –
Vacuole – – – – – – – – – – – – – – –

Images

Circle plot not available

Protein Concentration and Protein Localization Data

R1 R2 R3
G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase
Concentration 1.0697 0.6329 0.5465 0.2003 0.3077 0.6792 -0.2678 1.2897 0.7192 0.1966 0.1443 0.0958 0.3148 0.213 0.2566 0.7709 -0.0684 -0.1765
Actin 0.0383 0.0072 0.0142 0.0062 0.0024 0.0162 0.1159 0.0478 0.0175 0.1509 0.0063 0.0218 0.0401 0.0085 0.0408 0.1789 0.0609 0.0184
Bud 0.0011 0.0031 0.002 0.0014 0.0033 0.0017 0.0008 0.0059 0.0135 0.0017 0.0013 0.0021 0.0005 0.0002 0.0025 0.0032 0.0025 0.001
Bud Neck 0.0029 0.0018 0.0048 0.0008 0.0006 0.0031 0.0032 0.0006 0.0053 0.0006 0.0019 0.0041 0.001 0.0009 0.0024 0.0035 0.0156 0.0414
Bud Periphery 0.003 0.0055 0.005 0.0049 0.0098 0.0033 0.0012 0.0144 0.0191 0.0032 0.0055 0.0066 0.0008 0.0004 0.0062 0.0073 0.0055 0.0024
Bud Site 0.0062 0.0311 0.0164 0.0019 0.0021 0.0016 0.0148 0.0067 0.0281 0.0031 0.0016 0.0076 0.0034 0.0066 0.0204 0.0098 0.0105 0.0037
Cell Periphery 0.0018 0.0015 0.0017 0.0006 0.0013 0.0016 0.0016 0.0007 0.01 0.0004 0.0058 0.0047 0.0007 0.0005 0.0064 0.0009 0.0041 0.0026
Cytoplasm 0.0274 0.0433 0.036 0.0259 0.0464 0.038 0.0392 0.0048 0.0868 0.0081 0.1116 0.0434 0.0233 0.0224 0.114 0.0502 0.1246 0.033
Cytoplasmic Foci 0.11 0.0975 0.1233 0.0929 0.0665 0.0912 0.0918 0.0291 0.1137 0.1042 0.1131 0.156 0.1441 0.1825 0.1407 0.0382 0.0936 0.1133
Eisosomes 0.0011 0.0004 0.0009 0.0006 0.0002 0.0007 0.0011 0.0029 0.0031 0.0015 0.0013 0.0008 0.0007 0.0022 0.0038 0.0012 0.0011 0.001
Endoplasmic Reticulum 0.0083 0.0028 0.005 0.0029 0.0052 0.0038 0.0107 0.0028 0.0076 0.0065 0.0022 0.006 0.0039 0.0247 0.0049 0.006 0.0051 0.007
Endosome 0.1714 0.1578 0.1291 0.1491 0.2428 0.1341 0.0965 0.1228 0.0765 0.0648 0.0482 0.1014 0.1337 0.2198 0.1476 0.0577 0.1033 0.0998
Golgi 0.0676 0.0751 0.0604 0.0445 0.093 0.0725 0.0507 0.0237 0.0337 0.0575 0.0292 0.0497 0.0705 0.0175 0.0349 0.1474 0.0922 0.0836
Lipid Particles 0.0864 0.0215 0.0448 0.038 0.0176 0.058 0.0815 0.0276 0.0471 0.0858 0.0624 0.0266 0.1635 0.0297 0.0307 0.0079 0.0406 0.0279
Mitochondria 0.3008 0.4091 0.3944 0.4519 0.3834 0.396 0.2509 0.4763 0.2248 0.2152 0.3184 0.3966 0.2054 0.2585 0.2145 0.3623 0.2238 0.4383
None 0.0569 0.0557 0.06 0.1102 0.0376 0.0832 0.1981 0.1802 0.1795 0.0307 0.1799 0.0683 0.0688 0.1103 0.1479 0.1093 0.154 0.0519
Nuclear Periphery 0.0168 0.0013 0.0032 0.0012 0.0015 0.0021 0.0018 0.0088 0.0332 0.0098 0.0006 0.0008 0.0016 0.0086 0.0022 0.0005 0.0007 0.0066
Nucleolus 0.0017 0.001 0.0012 0.0005 0.0013 0.0008 0.0005 0.0018 0.0021 0.0009 0.0013 0.0009 0.0012 0.0002 0.0005 0.0001 0.0009 0.0005
Nucleus 0.0088 0.0016 0.0027 0.0008 0.0015 0.0018 0.0012 0.0037 0.0094 0.0013 0.0014 0.0008 0.0009 0.0008 0.001 0.0003 0.0012 0.0012
Peroxisomes 0.0571 0.0657 0.0763 0.0457 0.0426 0.0796 0.031 0.021 0.0673 0.238 0.1014 0.085 0.0969 0.0776 0.038 0.0116 0.0505 0.0592
Punctate Nuclear 0.008 0.001 0.0059 0.0012 0.0006 0.0014 0.0011 0.0025 0.0043 0.0113 0.0011 0.0009 0.0288 0.0009 0.0051 0.0006 0.0005 0.0009
Vacuole 0.0174 0.0114 0.0088 0.0128 0.0307 0.0058 0.0049 0.0086 0.0127 0.0019 0.0039 0.0095 0.0078 0.0206 0.0284 0.0014 0.0061 0.0035
Vacuole Periphery 0.007 0.0045 0.0038 0.0059 0.0097 0.0036 0.0016 0.0073 0.0047 0.0027 0.0015 0.0062 0.0025 0.0066 0.007 0.0017 0.003 0.0026

Sequencing Data

R1 R2
G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Post-START S/G2 Metaphase Anaphase Telophase
Gene Expression 7.8053 5.7862 7.577 9.5608 8.1453 8.3718 8.5175 7.8973 7.214 12.2871
Translational Efficiency – – – – – – – – – –

Hit Data

Dataset Hit
Protein Concentration ✘
Protein Localization ✘
Gene Expression ✘
Translational Efficiency –

Micrographs

Cell Count

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
404 835 121 105 490 1521 2161 319 894 2356 2282 424

Protein Abundance

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
Mean 799.41 585.28 918.21 859.33 547.23 731.21 742.20 800.71 661.19 679.49 751.53 815.23
Standard Deviation 103.33 94.05 126.32 110.97 78.02 93.51 100.54 109.19 154.64 116.84 109.43 112.51
Intensity Change Log 2 — -0.449809 0.199889 0.104277 — 0.418138 0.439661 0.549133 — -0.032668 0.302175 0.301854

Localization Score (DeepLoc)

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
Actin 0.007541 0.001343 0.009490 0.008575 0.000699 0.000812 0.005130 0.007769 0.003791 0.001000 0.005361 0.007968
Bud Neck 0.043369 0.043254 0.019442 0.039883 0.039069 0.019392 0.030886 0.026006 0.041012 0.027849 0.030280 0.029442
Bud Site 0.061604 0.031164 0.067482 0.068281 0.005732 0.009068 0.074533 0.047226 0.030981 0.016899 0.074159 0.052440
Cell Periphery 0.000486 0.000424 0.000455 0.000254 0.001332 0.000282 0.000172 0.000461 0.000950 0.000332 0.000187 0.000410
Cytoplasm 0.053299 0.040345 0.083753 0.084683 0.019212 0.018180 0.074799 0.049551 0.034616 0.026035 0.075274 0.058251
Cytoplasmic Foci 0.189844* 0.165484 0.209279* 0.193018* 0.093037 0.141497 0.176202 0.171338 0.136785 0.149998 0.177956 0.176707
Eisosomes 0.000293 0.000203 0.000218 0.000248 0.000537 0.000210 0.000137 0.000286 0.000427 0.000208 0.000141 0.000277
Endoplasmic Reticulum 0.001535 0.000622 0.011600 0.002797 0.001349 0.000420 0.002010 0.002753 0.001433 0.000491 0.002519 0.002764
Endosome 0.136566 0.128519 0.110449 0.105693 0.042602 0.117299 0.063804 0.128853 0.085065 0.121275 0.066278 0.123118
Golgi 0.046083 0.109423 0.040489 0.013271 0.067098 0.145023* 0.060438 0.072941 0.057601 0.132406* 0.059380 0.058164
Lipid Particles 0.007926 0.018601 0.019136 0.019018 0.027009 0.017019 0.004635 0.021833 0.018386 0.017580 0.005403 0.021136
Mitochondria 0.110693* 0.286144* 0.035233 0.024374 0.608492* 0.406405* 0.220533* 0.154002* 0.383536* 0.363783* 0.210708* 0.121901*
Mitotic Spindle 0.036026 0.015726 0.093117 0.035722 0.001043 0.007316 0.078834 0.044010 0.016852 0.010297 0.079592 0.041958
None 0.000463 0.009436 0.001250 0.000365 0.003267 0.000688 0.001040 0.001439 0.002000 0.003789 0.001051 0.001173
Nuclear Periphery 0.000311 0.000413 0.001693 0.001432 0.000432 0.000225 0.000368 0.001181 0.000378 0.000292 0.000438 0.001243
Nuclear Periphery Foci 0.000664 0.000721 0.013217 0.006211 0.000297 0.000306 0.000664 0.002587 0.000462 0.000453 0.001330 0.003484
Nucleolus 0.002804 0.009388 0.008658 0.004700 0.003717 0.001685 0.001277 0.006581 0.003304 0.004415 0.001668 0.006115
Nucleus 0.028217 0.012302 0.043614 0.083048 0.015822 0.005088 0.018110 0.022257 0.021423 0.007645 0.019462 0.037311
Peroxisomes 0.181124* 0.100518* 0.098752 0.141790* 0.053004 0.078221 0.106892* 0.170835* 0.110902* 0.086123 0.106460* 0.163643*
Vacuole 0.081603 0.017813 0.114630 0.159526 0.006586 0.019427 0.070948 0.056932 0.040486 0.018855 0.073264 0.082339
Vacuole Periphery 0.009546 0.008157 0.018042 0.007112 0.009662 0.011437 0.008587 0.011160 0.009610 0.010274 0.009088 0.010158

Localization Changes (T-score)

R1 R2 R1 & R2
UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14 UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14 UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14
Actin 2.85 -0.90 -0.03 -2.44 0.78 -0.41 -8.84 -5.17 -5.04 -1.79 2.79 -1.58 -2.59 -5.54 -1.77
Bud Neck -0.04 4.09 0.61 0.66 -1.69 3.47 1.30 2.05 -1.61 1.52 3.16 2.42 2.40 -0.16 0.70
Bud Site 4.37 -0.69 -0.13 -2.28 0.36 -3.00 -22.21* -7.60 -6.97 4.25 4.32 -10.42 -3.40 -6.32 3.70
Cell Periphery 0.24 0.03 1.06 3.24 3.09 4.12 4.57 3.35 -2.31 -4.00 3.59 4.46 3.06 -1.21 -3.98
Cytoplasm 2.31 -2.09 -2.49 -3.71 -0.48 0.26 -14.25 -4.58 -4.92 3.22 2.81 -9.80 -3.89 -5.70 2.11
Cytoplasmic Foci 2.15 -0.70 0.22 -1.05 0.71 -6.14 -10.83 -7.26 -3.20 0.50 -2.00 -5.92 -3.96 -2.91 0.24
Eisosomes 1.06 0.76 0.47 -1.50 -0.75 2.68 3.38 2.08 -1.77 -4.93 2.83 3.82 1.94 -2.22 -5.74
Endoplasmic Reticulum 3.11 -5.52 -2.62 -4.28 4.53 1.73 -1.39 -2.03 -4.86 -1.31 2.95 -3.33 -2.96 -6.09 -0.49
Endosome 0.59 1.96 2.41 2.20 0.71 -13.18 -4.71 -7.98 -0.83 -6.28 -6.26 3.48 -3.69 0.28 -6.32
Golgi -9.00 0.89 5.75 14.31 3.49 -10.18 1.37 -0.32 8.55 -1.54 -14.14 -0.09 0.19 11.20 0.28
Lipid Particles -4.63 -3.33 -1.60 0.25 0.40 2.26 5.48 1.05 -1.22 -5.12 0.29 5.54 -0.56 -0.90 -5.24
Mitochondria -11.35 5.62 6.86 20.01 1.17 11.10 23.16 21.96 16.03 4.47 1.33 12.51 15.76 19.27 7.30
Mitotic Spindle 3.14 -3.19 -0.23 -2.58 2.86 -6.55 -22.13* -6.12 -5.23 3.74 2.15 -13.91 -4.20 -5.66 4.86
None -4.70 -2.11 -0.33 4.67 1.86 1.92 1.65 0.53 -1.25 -0.98 -2.01 1.23 0.12 1.86 -0.83
Nuclear Periphery -0.19 -3.19 -2.52 -2.33 0.34 1.05 0.08 -1.60 -2.18 -1.76 0.91 -0.67 -2.38 -2.77 -2.24
Nuclear Periphery Foci -0.14 -2.86 -1.46 -1.45 1.42 -0.19 -2.52 -2.28 -2.27 -1.75 0.16 -3.22 -2.52 -2.54 -1.58
Nucleolus -3.76 -1.83 -1.61 2.39 0.96 1.32 1.48 -1.44 -2.27 -2.35 -1.74 1.44 -1.84 -0.92 -2.56
Nucleus 3.19 -1.94 -4.11 -5.41 -2.84 2.96 -1.15 -1.69 -4.57 -1.09 4.60 0.17 -3.44 -6.64 -3.93
Peroxisomes 5.67 4.49 2.20 -1.22 -1.21 -4.29 -9.05 -8.19 -6.48 -4.42 3.21 0.42 -3.74 -6.12 -4.36
Vacuole 9.69 -2.30 -4.70 -8.73 -2.45 -6.80 -22.58 -8.40 -6.30 2.36 6.64 -7.79 -5.91 -9.95 -1.53
Vacuole Periphery 0.67 -1.76 1.27 0.99 2.45 -1.10 0.83 -0.46 0.34 -1.24 -0.63 0.55 -0.04 0.43 -0.48
Standard name
Human Ortholog
Description Specific translational activator for the COX1 mRNA; binds to the COX1 mRNA; also influences stability of intron-containing COX1 primary transcripts; localizes to the mitochondrial inner membrane; contains 12 pentatricopeptide repeats (PPRs)
Localization
Cell Percentages mitochondrion (54%)
Cell Cycle Regulation No
Subcompartmental Group mito-1

Pet309

Pet309


Nearest neighbours ( genes)

correlation threshold

0.6

1.0

0.80


Biological Process (Gene Ontology)
Molecular Function (Gene Ontology)
Cellular Component (Gene Ontology)

Endocytosis
Temp Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Vph1-GFP)
37℃
RT
Cell Cycle Omics

CYCLoPs (Pet309-GFP)
Gene / Allele Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Sac6-tdTomato)
Gene Images
Gene Images
Images are not yet available
Images are not yet available