Standard name
Human Ortholog
Description Protein that inhibits Doa4p deubiquitinating activity; contributes to ubiquitin homeostasis by regulating the conversion of free ubiquitin chains to ubiquitin monomers by Doa4p; GFP-fusion protein localizes to endosomes

Micrographs

ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0.07 0 0 0 0 0 0 0 0 0 0 0 0 0.05 0 0 0 0 0 0 0
Bud 0 0 0 0 0 0 0 0 0 0.08 0.07 0 0 0 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cell Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cytoplasm 0.11 0.21 0.2 0.09 0.33 0.81 0.88 0.85 0.83 0.73 0.74 0.11 0.28 0.42 0.39 0.36 0.32 0 0 0 0 0 0
Endoplasmic Reticulum 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0.06 0 0 0 0 0 0 0 0
Endosome 0.81 0.62 0.46 0.68 0.37 0 0.05 0 0 0 0 0.75 0.65 0.53 0.41 0.45 0.5 0.87 0.81 0.79 0.82 0.78 0.75
Golgi 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Mitochondria 0 0 0 0 0 0.17 0 0.15 0.12 0.19 0.23 0 0 0 0 0 0 0 0 0 0 0 0.06
Nucleus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nuclear Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0.06 0 0 0 0 0 0 0 0 0.13 0.1 0 0 0 0 0 0 0 0 0
SpindlePole 0.14 0.1 0.4 0.45 0.09 0 0 0 0.05 0.08 0.08 0.2 0.19 0.16 0.16 0.12 0.12 0 0.05 0.07 0.05 0 0
Vac/Vac Membrane 0.28 0.26 0.17 0.06 0.48 0.08 0.09 0 0 0 0 0.09 0.07 0.08 0.3 0.28 0.25 0 0 0 0 0 0.06
ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 3 4 16 5 1 0 0 1 0 1 1 5 2 0 13 12 4 2 2 2 2 8 3
Bud 1 3 3 6 2 4 7 8 16 17 20 1 6 2 1 3 1 1 1 0 1 2 1
Bud Neck 0 0 0 1 0 0 3 0 4 4 1 0 1 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 1 0 1 0 1 1 0 3 0 0 0 0 0 0
Cell Periphery 0 0 0 0 0 0 1 3 0 0 1 0 0 0 0 0 0 0 0 0 0 0 0
Cytoplasm 20 35 48 23 101 182 387 228 303 153 226 23 85 148 119 88 63 3 6 12 7 15 4
Endoplasmic Reticulum 2 4 2 2 0 1 0 1 1 1 1 7 7 1 17 9 7 0 0 0 1 5 6
Endosome 144 101 113 168 112 5 21 4 10 4 4 162 202 185 124 112 98 169 148 247 237 331 260
Golgi 1 2 0 0 1 0 0 0 0 0 0 1 0 3 1 0 3 4 3 0 5 11 10
Mitochondria 1 5 0 5 3 39 12 39 43 40 70 3 3 0 3 1 5 2 4 10 5 10 21
Nucleus 0 0 0 1 2 2 3 0 0 2 1 0 0 0 0 0 0 0 0 0 0 2 1
Nuclear Periphery 0 1 0 0 0 0 0 0 0 0 1 1 0 0 0 0 1 0 0 0 0 2 2
Nucleolus 7 2 1 8 0 1 0 0 0 0 0 1 0 0 2 0 0 0 0 0 1 0 0
Peroxisomes 3 0 0 15 1 0 1 0 0 1 0 2 39 36 2 0 0 0 0 1 2 3 2
SpindlePole 25 16 97 111 27 3 17 10 20 16 25 44 58 55 50 30 24 7 8 21 15 12 4
Vac/Vac Membrane 50 42 41 15 145 17 41 3 10 7 7 20 23 28 90 70 50 0 5 13 6 14 22
Unique Cell Count 178 164 245 247 302 226 441 267 366 210 307 217 309 349 305 247 197 194 184 314 290 425 349
Labelled Cell Count 257 215 321 361 395 255 493 298 408 246 361 270 426 458 422 325 256 194 184 314 290 425 349


Endosome

(Huh et al. Nature 2003 425:686)
Screen WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 AF100 AF140 AF180
Mean Cell GFP Intensity (1e-4) 7.3 7.2 6.6 6.1 6.3 3.9 4.8 4.0 4.1 3.8 4.0 6.1 6.2 6.2 8.0 8.5 7.8 6.6 6.3 6.6
Std Deviation (1e-4) 1.3 1.1 1.1 1.2 1.6 1.0 1.5 1.2 1.5 1.0 1.3 1.2 1.4 1.7 1.1 1.0 1.1 1.7 1.9 1.9
Intensity Change (Log2) -0.12 -0.06 -0.77 -0.45 -0.73 -0.68 -0.81 -0.71 -0.13 -0.1 -0.1 0.27 0.35 0.23 -0.01 -0.07 -0.01


Localization RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3
Cortical Patches -2.5 -4.2 0 0 0 0 0 0 -2.2 -3.9 -4.8 -1.2 -0.8 -2.3
Bud 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cell Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cytoplasm -3.2 3.6 13.2 17.8 14.9 15.5 11.4 12.6 -2.7 2.2 5.8 4.9 4.0 3.0
Endoplasmic Reticulum 0 0 0 0 0 0 0 0 0 0 0 3.0 2.1 0
Endosome 4.9 -2.1 0 0 0 0 0 0 6.2 4.5 1.7 -1.3 -0.2 0.8
Golgi 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Mitochondria 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleus 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nuclear Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Peroxisomes 3.9 0 0 0 0 0 0 0 0 5.8 5.2 0 0 0
SpindlePole 1.2 -8.5 0 0 0 0 0 0 -4.5 -5.4 -6.6 -6.1 -7.0 -6.4
Vacuole -3.7 7.7 -3.0 -2.9 -6.3 -6.1 -4.6 -6.0 -2.4 -3.4 -3.3 3.5 3.1 2.2

Images

Circle plot not available

Protein Concentration and Protein Localization Data

R1 R2 R3
G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase
Concentration 0.9641 1.1512 0.9574 0.3125 1.2288 0.9299 1.9405 2.8125 2.9354 3.043 2.8468 2.9566 3.8174 5.329 4.6308 5.7314 3.6372 4.5675
Actin 0.019 0.0043 0.0184 0 0.0579 0.0001 0.0044 0.0027 0.0061 0.0019 0.0198 0.0157 0.0039 0.0006 0.0023 0 0.0024 0.0004
Bud 0.0025 0.0006 0.0004 0.0001 0.0007 0 0.0003 0.0001 0.0006 0.0001 0.0023 0.0002 0.0001 0.0001 0.0001 0 0.0835 0
Bud Neck 0.0016 0.0003 0.0004 0.0001 0.0025 0.0003 0.0005 0.0007 0.0003 0.0005 0.0007 0.0006 0.0002 0.0001 0.0005 0 0.0006 0.0003
Bud Periphery 0.0034 0.0001 0.0004 0 0.0009 0 0.0003 0 0.0004 0 0.0015 0.0004 0 0 0.0001 0 0.0469 0
Bud Site 0.0122 0.0036 0.0025 0.0002 0.0017 0.0001 0.0015 0.001 0.003 0.0002 0.0167 0.0002 0.0003 0.0005 0.001 0 0.0038 0.0001
Cell Periphery 0.0005 0.0001 0.0001 0 0.0003 0 0.0001 0.0001 0.0001 0 0.0001 0 0.0001 0.0001 0.0002 0 0.0012 0
Cytoplasm 0.0175 0.0915 0.0671 0.1624 0.0078 0.1425 0.0599 0.1071 0.1295 0.0997 0.0121 0.0383 0.1137 0.1023 0.0569 0.0018 0.0217 0.0937
Cytoplasmic Foci 0.2631 0.3325 0.3337 0.1146 0.2776 0.3273 0.3185 0.3293 0.3045 0.3012 0.2746 0.2576 0.333 0.2655 0.3426 0.7958 0.4176 0.3362
Eisosomes 0.0002 0 0.0001 0 0.0004 0 0.0001 0.0001 0.0001 0 0.0001 0 0 0 0.0001 0 0 0
Endoplasmic Reticulum 0.0025 0.0057 0.0056 0.0029 0.0012 0.0066 0.003 0.0049 0.0044 0.0019 0.0027 0.001 0.0072 0.0043 0.0052 0 0.0006 0.0059
Endosome 0.316 0.4553 0.4003 0.5441 0.3449 0.3539 0.3666 0.3787 0.3701 0.3669 0.4523 0.3886 0.3204 0.3482 0.318 0.069 0.3345 0.399
Golgi 0.0563 0.023 0.0326 0.0734 0.1483 0.0378 0.0364 0.0332 0.0415 0.0127 0.1341 0.0444 0.0349 0.0465 0.0411 0.0108 0.0185 0.0372
Lipid Particles 0.1371 0.0315 0.0587 0.0024 0.0735 0.077 0.0674 0.0255 0.0276 0.0096 0.0214 0.0419 0.0447 0.0523 0.0526 0.008 0.0131 0.0555
Mitochondria 0.0452 0.0022 0.0022 0.0007 0.0386 0.0009 0.0048 0.0007 0.0029 0.0004 0.0176 0.0016 0.001 0.0008 0.0016 0.0001 0.0162 0.0008
None 0.041 0.0188 0.0337 0.0043 0.0002 0.0161 0.0308 0.0237 0.0441 0.009 0.0014 0.0098 0.0406 0.1166 0.0691 0.0001 0.0118 0.0091
Nuclear Periphery 0.0025 0.0026 0.002 0.0011 0.0005 0.001 0.0015 0.0023 0.0039 0.008 0.0015 0.0101 0.006 0.0009 0.0009 0 0.0008 0.0009
Nucleolus 0.0006 0.0003 0.0004 0.0001 0.0001 0.0001 0.0007 0.0002 0.0003 0.0002 0.0005 0.0004 0.0004 0.0001 0.0005 0 0.0021 0.0001
Nucleus 0.0012 0.0006 0.0004 0.0007 0.0002 0.0006 0.0239 0.0355 0.0026 0.1408 0.0005 0.1074 0.0017 0.0003 0.0005 0 0.0048 0.0006
Peroxisomes 0.0501 0.0131 0.0334 0.0018 0.0363 0.029 0.067 0.035 0.0398 0.0327 0.0291 0.0619 0.051 0.0442 0.0768 0.113 0.0115 0.0515
Punctate Nuclear 0.0055 0.0014 0.0022 0.0003 0.001 0.0016 0.0038 0.0029 0.0027 0.0076 0.0017 0.0023 0.0371 0.0008 0.0098 0.0012 0.0016 0.0015
Vacuole 0.019 0.01 0.0042 0.0719 0.0033 0.0039 0.0074 0.0146 0.0128 0.0047 0.007 0.0075 0.003 0.014 0.0185 0.0001 0.0052 0.0056
Vacuole Periphery 0.0029 0.0026 0.0011 0.0189 0.0022 0.0012 0.0013 0.0017 0.0026 0.0018 0.0023 0.0098 0.0007 0.0019 0.0016 0 0.0017 0.0015

Sequencing Data

R1 R2
G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Post-START S/G2 Metaphase Anaphase Telophase
Gene Expression 31.1076 29.4441 26.704 34.7699 48.6361 29.2615 26.8765 31.2563 19.5204 34.8278
Translational Efficiency 0.9517 1.0568 1.0574 0.5738 0.5603 0.6002 0.5757 0.5047 0.6402 0.5938

Hit Data

Dataset Hit
Protein Concentration
Protein Localization
Gene Expression
Translational Efficiency

Micrographs

Cell Count

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
1775 463 1559 1279 1322 1938 2489 1071 3097 2401 4048 2350

Protein Abundance

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
Mean 802.36 856.60 988.84 904.89 726.72 892.01 969.20 893.33 770.07 885.18 976.76 899.62
Standard Deviation 95.74 189.80 117.12 124.09 93.88 114.85 117.20 132.01 102.05 133.37 117.56 127.89
Intensity Change Log 2 0.094372 0.301487 0.173493 0.295660 0.415395 0.297794 0.193545 0.356746 0.233906

Localization Score (DeepLoc)

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
Actin 0.000334 0.001595 0.003344 0.002919 0.000330 0.000318 0.002761 0.001406 0.000332 0.000564 0.002985 0.002230
Bud Neck 0.004110 0.006805 0.017793 0.005584 0.005750 0.005490 0.004008 0.012256 0.004810 0.005743 0.009317 0.008625
Bud Site 0.003065 0.014397 0.027212 0.017925 0.002466 0.003329 0.013565 0.024332 0.002810 0.005463 0.018821 0.020845
Cell Periphery 0.000023 0.000073 0.000072 0.000040 0.000027 0.000054 0.000028 0.000130 0.000025 0.000058 0.000045 0.000081
Cytoplasm 0.007064 0.019222 0.029777 0.010606 0.010669 0.007293 0.009550 0.029877 0.008603 0.009593 0.017340 0.019388
Cytoplasmic Foci 0.165758 0.250509* 0.189804* 0.226787* 0.226057* 0.158031 0.292354* 0.177432 0.191498* 0.175864 0.252859* 0.204294*
Eisosomes 0.000024 0.000050 0.000039 0.000020 0.000044 0.000028 0.000017 0.000029 0.000032 0.000032 0.000025 0.000024
Endoplasmic Reticulum 0.000125 0.000349 0.002675 0.000589 0.000193 0.000412 0.000428 0.000858 0.000154 0.000399 0.001293 0.000712
Endosome 0.684678* 0.520771* 0.262498* 0.478143* 0.567676* 0.665002* 0.442462* 0.456303* 0.634734* 0.637189* 0.373153* 0.468189*
Golgi 0.034584 0.047984 0.008263 0.022192 0.064105 0.047298 0.024045 0.017005 0.047185 0.047430 0.017967 0.019828
Lipid Particles 0.015048 0.019138 0.021529 0.012104 0.029664 0.022797 0.020267 0.011678 0.021287 0.022091 0.020753 0.011910
Mitochondria 0.005257 0.002632 0.002716 0.002086 0.006216 0.005022 0.001143 0.005319 0.005667 0.004561 0.001749 0.003560
Mitotic Spindle 0.006549 0.010483 0.209515* 0.067388 0.004078 0.006947 0.051579 0.073843 0.005494 0.007629 0.112405* 0.070330
None 0.000072 0.000411 0.000436 0.000165 0.000342 0.000490 0.000014 0.002159 0.000187 0.000475 0.000177 0.001074
Nuclear Periphery 0.000079 0.000125 0.003267 0.000518 0.000062 0.000137 0.000187 0.000901 0.000072 0.000135 0.001374 0.000693
Nuclear Periphery Foci 0.001549 0.001835 0.021571 0.005488 0.001260 0.002110 0.003833 0.006031 0.001425 0.002057 0.010664 0.005735
Nucleolus 0.000423 0.002079 0.004315 0.000504 0.000567 0.001735 0.000138 0.002705 0.000485 0.001801 0.001747 0.001507
Nucleus 0.001097 0.005325 0.013065 0.000667 0.000823 0.001571 0.000166 0.004673 0.000980 0.002295 0.005134 0.002493
Peroxisomes 0.049326 0.072921 0.053469 0.080662 0.068398 0.046932 0.102574* 0.070652 0.057467 0.051943 0.083662 0.076100
Vacuole 0.019516 0.021547 0.127098 0.062168 0.010204 0.022765 0.030243 0.094788 0.015541 0.022530 0.067545 0.077035
Vacuole Periphery 0.001318 0.001750 0.001543 0.003445 0.001067 0.002241 0.000637 0.007622 0.001211 0.002146 0.000986 0.005349

Localization Changes (T-score)

R1 R2 R1 & R2
UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14 UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14 UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14
Actin -2.53 -4.74 -3.06 -1.26 0.34 0.13 -6.75 -2.47 -2.51 2.88 -1.85 -8.11 -3.79 -3.26 1.58
Bud Neck -1.72 -15.49 -3.33 0.02 13.45 0.20 1.02 -5.04 -5.87 -6.95 -1.26 -10.39 -6.13 -4.81 3.12
Bud Site -4.43 -14.65 -8.38 -1.53 3.90 -1.88 -13.39 -10.21 -9.80 -4.14 -4.20 -19.41 -13.09 -10.69 -0.77
Cell Periphery -3.12 -11.45 -4.02 2.01 7.33 -2.60 -1.32 -2.06 -1.39 -1.97 -3.71 -9.44 -2.56 -0.80 -1.22
Cytoplasm -3.18 -13.33 -4.59 0.84 8.90 1.81 -1.03 -7.44 -8.74 -7.23 -0.78 -10.71 -8.76 -7.79 -0.81
Cytoplasmic Foci -9.28 1.64 -8.29 3.65 -9.41 11.10 -9.26 9.15 -1.04 20.51 3.51 -10.42 -0.04 -3.38 9.80
Eisosomes -2.99 -7.62 0.79 3.36 12.52 3.71 6.87 2.25 -2.21 -7.80 -0.02 1.36 2.01 1.94 1.26
Endoplasmic Reticulum -2.73 -12.94 -2.61 -1.33 9.44 -2.03 -1.14 -3.80 -2.50 -1.48 -2.98 -8.48 -4.49 -2.37 4.37
Endosome 11.23 56.57* 23.30 4.63 -24.14* -9.36 14.28 13.40 23.46 2.41 -0.28 43.47* 26.16 24.96 -10.98
Golgi -3.19 17.77 7.12 6.81 -9.55 4.94 14.37 16.70 13.98 5.67 -0.08 19.35 17.08 14.83 -0.97
Lipid Particles -1.25 -3.69 1.61 2.20 6.54 2.34 3.34 7.21 5.59 6.20 -0.43 0.05 6.47 6.13 8.72
Mitochondria 2.41 2.49 3.22 0.80 1.65 1.02 5.11 1.29 0.48 -3.52 1.28 5.54 3.08 2.03 -2.61
Mitotic Spindle -1.52 -26.47* -13.17 -11.45 13.56 -2.23 -16.91 -13.38 -12.86 -4.18 -1.83 -29.67 -18.73 -18.01 5.78
None -1.80 -3.76 -1.00 1.27 2.58 -0.61 2.09 -1.81 -1.62 -2.21 -1.58 -0.08 -1.93 -1.16 -1.94
Nuclear Periphery -1.04 -13.11 -7.39 -6.37 11.45 -2.50 -8.35 -4.87 -4.54 -4.00 -2.42 -13.41 -7.26 -6.66 6.65
Nuclear Periphery Foci -0.11 -13.92 -4.00 -4.13 9.86 -1.69 -4.94 -5.85 -4.76 -2.89 -0.89 -13.50 -6.62 -6.02 5.16
Nucleolus -1.94 -8.02 -1.95 1.41 7.28 -2.74 2.76 -5.30 -2.34 -6.26 -3.49 -6.93 -5.61 -0.31 1.43
Nucleus -2.20 -11.26 -0.74 2.02 11.10 -1.82 1.49 -4.98 -4.11 -5.26 -2.50 -10.33 -4.63 -1.87 5.75
Peroxisomes -3.48 1.81 -5.59 -0.45 -7.18 4.81 -6.27 1.10 -3.37 7.25 1.76 -6.32 -3.45 -4.95 2.20
Vacuole -0.79 -28.86 -13.47 -11.24 12.65 -6.38 -13.27 -18.87 -16.85 -13.90 -3.97 -27.94 -22.80 -20.54 -3.91
Vacuole Periphery -1.67 -0.76 -3.21 -2.32 -3.06 -4.83 2.87 -4.16 -3.22 -4.52 -4.96 2.77 -5.18 -3.68 -5.62
Standard name
Human Ortholog
Description Protein that inhibits Doa4p deubiquitinating activity; contributes to ubiquitin homeostasis by regulating the conversion of free ubiquitin chains to ubiquitin monomers by Doa4p; GFP-fusion protein localizes to endosomes
Localization
Cell Percentages endosome (80%)
Cell Cycle Regulation No
Subcompartmental Group N/A

Rfu1

Rfu1


Nearest neighbours ( genes)

correlation threshold

0.6

1.0

0.80


Biological Process (Gene Ontology)

Molecular Function (Gene Ontology)

Cellular Component (Gene Ontology)


Endocytosis
Temp Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Vph1-GFP)
37℃
RT
Cell Cycle Omics

CYCLoPs (Rfu1-GFP)
Gene / Allele Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Sac6-tdTomato)
Gene Images
Gene Images
Images are not yet available
Images are not yet available