Standard name
Human Ortholog
Description Cyclin-dependent kinase inhibitor (CKI); inhibitor of Cdc28-Clb kinase complexes that controls G1/S phase transition, preventing premature S phase and ensuring genomic integrity; phosphorylated by Clb5/6-Cdk1 and Cln1/2-Cdk1 kinase which regulate timing of Sic1p degradation; phosphorylation targets Sic1p for SCF(CDC4)-dependent turnover; functional homolog of mammalian Kip1
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Micrographs

ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 – – – 0 0 0 0 0 0
Bud 0 0 0 0 0 0 0 – 0.09 0 0 0 0 0 0 – – – 0.12 0.05 0.09 0.05 0.08 0.06
Bud Neck 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 – – – 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 – – – – – – – – –
Cell Periphery 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 – – – 0 0 0 0 0 0
Cytoplasm 0.64 0.77 0.75 0.76 0.77 0.61 0.53 – 0.37 0.44 0.31 0.38 0.83 0.78 0.87 – – – 0.39 0.52 0.5 0.27 0.24 0.3
Endoplasmic Reticulum 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 – – – 0 0 0 0 0 0
Endosome 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 – – – 0 0 0 0 0 0
Golgi 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 – – – 0 0 0 0 0 0
Mitochondria 0 0 0 0 0 0 0 – 0.37 0.2 0.19 0.17 0 0 0 – – – 0 0 0 0.11 0.12 0.09
Nucleus 0.21 0.14 0.19 0.2 0.13 0.26 0.36 – 0.45 0.29 0.43 0.46 0.12 0.15 0.07 – – – 0.28 0.18 0.2 0.32 0.34 0.25
Nuclear Periphery 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 – – – 0 0 0 0 0 0
Nucleolus 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 – – – 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 – – – 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 – – – 0.07 0.06 0.08 0.06 0 0
Vac/Vac Membrane 0.15 0.13 0.09 0 0.09 0.11 0.07 – 0 0.12 0.12 0.08 0.09 0.08 0.14 – – – 0.05 0.06 0 0.08 0.12 0.11
ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 1 – 0 0 0 0 0 0 0 – – – 0 1 2 0 0 1
Bud 0 0 2 0 2 2 7 – 12 4 5 5 0 0 0 – – – 16 14 29 5 13 10
Bud Neck 0 0 4 1 1 2 0 – 2 2 2 0 0 0 0 – – – 0 1 3 1 3 1
Bud Site 0 0 0 0 0 0 0 – 0 1 0 4 0 0 0 – – – – – – – – –
Cell Periphery 0 2 1 0 0 1 0 – 0 0 1 1 0 0 0 – – – 0 1 1 0 0 0
Cytoplasm 84 196 225 135 167 178 164 – 51 83 66 104 75 113 103 – – – 55 145 157 30 42 48
Endoplasmic Reticulum 0 0 0 0 1 0 2 – 0 0 0 0 0 2 0 – – – 0 0 2 2 2 1
Endosome 3 0 8 1 2 7 4 – 0 1 0 2 0 1 3 – – – 2 8 3 1 2 6
Golgi 2 0 0 0 0 0 0 – 0 0 0 0 0 0 0 – – – 3 3 3 2 0 1
Mitochondria 5 6 0 3 5 6 13 – 52 38 40 48 3 1 1 – – – 2 10 5 12 20 14
Nucleus 27 36 58 36 29 75 111 – 62 55 93 127 11 22 8 – – – 39 50 63 36 59 40
Nuclear Periphery 0 0 0 1 0 1 0 – 0 0 0 1 0 1 0 – – – 0 0 0 0 1 1
Nucleolus 1 1 0 1 0 0 2 – 3 3 9 9 0 0 0 – – – 1 2 1 0 2 2
Peroxisomes 0 0 1 0 0 1 0 – 0 1 1 0 0 0 0 – – – 0 0 0 0 0 1
SpindlePole 0 1 1 0 2 4 4 – 0 2 3 3 0 0 0 – – – 10 17 24 6 2 6
Vac/Vac Membrane 20 33 27 7 20 31 22 – 5 23 27 21 8 12 17 – – – 7 16 14 8 20 18
Unique Cell Count 131 256 302 178 216 290 312 139 187 216 276 90 145 119 144 279 317 112 174 162
Labelled Cell Count 142 275 327 185 229 308 330 187 213 247 325 97 152 132 144 279 317 112 174 162


Cytoplasm, Nucleus

(Huh et al. Nature 2003 425:686)
Screen WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 AF100 AF140 AF180
Mean Cell GFP Intensity (1e-4) 5.4 4.5 6.1 6.1 6.3 5.6 5.8 – 4.9 5.0 5.8 5.5 5.5 6.5 5.8 – – – 7.3 7.7 7.4
Std Deviation (1e-4) 2.1 1.6 1.7 2.7 2.4 2.4 2.3 – 1.8 1.8 2.8 1.9 1.6 2.6 2.2 – – – 2.5 2.4 2.4
Intensity Change (Log2) – – – 0.0 0.04 -0.13 -0.08 – -0.33 -0.27 -0.06 -0.15 -0.15 0.08 -0.06 – – – 0.26 0.33 0.27


Localization RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3
Actin – – – – – – – – – – – – – – –
Bud – – – – – – – – – – – – – – –
Bud Neck – – – – – – – – – – – – – – –
Bud Site – – – – – – – – – – – – – – –
Cell Periphery – – – – – – – – – – – – – – –
Cyto – – – – – – – – – – – – – – –
Endoplasmic Reticulum – – – – – – – – – – – – – – –
Endosome – – – – – – – – – – – – – – –
Golgi – – – – – – – – – – – – – – –
Mitochondria – – – – – – – – – – – – – – –
Nuclear Periphery – – – – – – – – – – – – – – –
Nuc – – – – – – – – – – – – – – –
Nucleolus – – – – – – – – – – – – – – –
Peroxisomes – – – – – – – – – – – – – – –
SpindlePole – – – – – – – – – – – – – – –
Vac – – – – – – – – – – – – – – –
Cortical Patches – – – – – – – – – – – – – – –
Cytoplasm – – – – – – – – – – – – – – –
Nucleus – – – – – – – – – – – – – – –
Vacuole – – – – – – – – – – – – – – –

Images

Circle plot not available

Protein Concentration and Protein Localization Data

R1 R2 R3
G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase
Concentration 5.707 4.9818 4.1039 3.5103 4.1028 4.8315 4.7635 5.287 3.3161 2.9439 4.7475 4.3299 3.5057 1.5431 1.0216 1.0145 0.7 1.9656
Actin 0.053 0.014 0.0083 0.0214 0.1249 0.0026 0.0121 0.0001 0.0184 0.0003 0.0005 0.0003 0.1495 0.0011 0.032 0.0461 0.0204 0.0298
Bud 0.0013 0.0123 0.0173 0.004 0.0023 0.0022 0.0005 0 0.0006 0.0012 0.0005 0.0008 0.001 0.0056 0.0176 0.0017 0.0006 0.0011
Bud Neck 0.004 0.0016 0.0017 0.0409 0.0013 0.0069 0.0003 0 0.0013 0.0011 0.0009 0.0019 0.0033 0.0004 0.0012 0.0012 0.001 0.0021
Bud Periphery 0.0016 0.008 0.0151 0.0059 0.0028 0.0023 0.0007 0 0.0009 0.0012 0.0003 0.0011 0.0015 0.0034 0.014 0.0017 0.0004 0.0012
Bud Site 0.0166 0.0305 0.0071 0.023 0.002 0.0005 0.0023 0 0.0036 0.0009 0.0014 0.0003 0.0119 0.0039 0.0054 0.0008 0.0024 0.0018
Cell Periphery 0.0005 0.001 0.001 0.0005 0.0003 0.0001 0.0004 0.0001 0.0003 0.0009 0.0002 0.0001 0.0005 0.0006 0.0006 0.0002 0.0001 0.0001
Cytoplasm 0.2418 0.2537 0.1707 0.2429 0.2229 0.3704 0.1907 0.106 0.1762 0.2119 0.3162 0.2317 0.0947 0.4007 0.2311 0.132 0.1232 0.3282
Cytoplasmic Foci 0.0339 0.0038 0.0236 0.0222 0.0039 0.0036 0.0265 0.0023 0.0199 0.0088 0.0017 0.0175 0.0173 0.0072 0.0047 0.0094 0.017 0.0061
Eisosomes 0.0006 0.0001 0.0004 0.0002 0.0008 0 0.0003 0.0003 0.0005 0.0003 0 0 0.0026 0.0001 0.0002 0.0002 0.0002 0.0001
Endoplasmic Reticulum 0.0098 0.0074 0.0081 0.002 0.0165 0.0035 0.0205 0.0022 0.0027 0.0056 0.0071 0.0037 0.0076 0.0059 0.0056 0.0049 0.0031 0.003
Endosome 0.0663 0.0161 0.0194 0.0189 0.015 0.0024 0.1498 0.0008 0.0201 0.0025 0.0024 0.0105 0.0168 0.0019 0.0039 0.0082 0.0345 0.0073
Golgi 0.0279 0.0006 0.0123 0.0425 0.0455 0.0001 0.012 0.0002 0.0031 0.0001 0.0001 0.0003 0.0128 0.0002 0.0015 0.0037 0.0095 0.0057
Lipid Particles 0.0139 0.0005 0.0228 0.014 0.0066 0.0001 0.0263 0.0002 0.0194 0.0016 0.0002 0.0006 0.01 0.001 0.0009 0.0035 0.0032 0.003
Mitochondria 0.0035 0.0018 0.0051 0.0183 0.0215 0.0008 0.0044 0.0001 0.0022 0.0002 0.0004 0.0004 0.0034 0.002 0.0036 0.0012 0.0025 0.0012
None 0.2543 0.3746 0.5219 0.5204 0.4441 0.2756 0.5003 0.7208 0.7067 0.7189 0.5511 0.3792 0.1274 0.4341 0.5662 0.75 0.7279 0.3352
Nuclear Periphery 0.016 0.0084 0.028 0.0014 0.0198 0.0129 0.0248 0.0002 0.0022 0.0022 0.0044 0.0063 0.029 0.005 0.0088 0.0055 0.0059 0.0078
Nucleolus 0.0021 0.0025 0.006 0.0004 0.0007 0.0028 0.0011 0.0003 0.002 0.0048 0.0009 0.0015 0.0027 0.0018 0.0011 0.0004 0.0053 0.0018
Nucleus 0.2069 0.2456 0.0913 0.0042 0.0583 0.3007 0.0044 0.166 0.0019 0.017 0.1058 0.3303 0.4633 0.1176 0.0743 0.0203 0.0107 0.2518
Peroxisomes 0.009 0.0002 0.0093 0.0113 0.0012 0.0001 0.0039 0 0.0067 0.0001 0 0.0003 0.012 0.0002 0.0005 0.0036 0.0035 0.0025
Punctate Nuclear 0.0319 0.0058 0.0201 0.0023 0.003 0.0095 0.0081 0.0001 0.0078 0.0105 0.0015 0.011 0.0299 0.0039 0.0218 0.003 0.0267 0.0087
Vacuole 0.0035 0.0096 0.0081 0.0022 0.0044 0.0023 0.0067 0.0002 0.0031 0.0098 0.0039 0.0018 0.0014 0.0028 0.0039 0.0018 0.0012 0.0012
Vacuole Periphery 0.0015 0.0018 0.0024 0.0007 0.0023 0.0005 0.0038 0 0.0004 0.0004 0.0005 0.0003 0.0014 0.0005 0.001 0.0005 0.0005 0.0003

Sequencing Data

R1 R2
G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Post-START S/G2 Metaphase Anaphase Telophase
Gene Expression 12.26 9.2293 46.2274 99.0732 45.1772 23.2167 16.3305 22.4375 75.2048 57.4961
Translational Efficiency 2.0215 1.5385 1.4527 1.3971 1.2847 1.2276 1.1266 1.3571 1.6375 1.419

Hit Data

Dataset Hit
Protein Concentration ✔
Protein Localization ✘
Gene Expression ✔
Translational Efficiency ✘

Micrographs

Cell Count

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
1251 884 3334 1989 767 1732 129 1447 2018 2616 3463 3436

Protein Abundance

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
Mean 696.63 835.28 933.77 840.75 657.71 835.18 900.09 849.03 681.84 835.21 932.52 844.24
Standard Deviation 113.23 147.91 203.92 180.96 105.37 134.79 139.66 168.67 111.92 139.36 202.00 175.94
Intensity Change Log 2 — 0.261867 0.422675 0.271284 — 0.344636 0.452618 0.368364 — 0.302655 0.437294 0.319245

Localization Score (DeepLoc)

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
Actin 0.000096 0.000336 0.000241 0.000650 0.000095 0.000174 0.000372 0.001166 0.000096 0.000229 0.000246 0.000868
Bud Neck 0.010941 0.021357 0.022706 0.015621 0.011476 0.018902 0.002064 0.010538 0.011144 0.019732 0.021937 0.013481
Bud Site 0.004101 0.015436 0.015765 0.033107 0.004150 0.007481 0.001481 0.034142 0.004120 0.010169 0.015232 0.033543
Cell Periphery 0.000510 0.000493 0.000264 0.000628 0.000375 0.000440 0.000332 0.000386 0.000458 0.000458 0.000267 0.000526
Cytoplasm 0.374009* 0.334732* 0.270680* 0.391299* 0.371760* 0.307708* 0.205684* 0.400984* 0.373155* 0.316840* 0.268259* 0.395377*
Cytoplasmic Foci 0.079655 0.086342 0.001338 0.014246 0.077259 0.050895 0.029792 0.018319 0.078744 0.062873 0.002398 0.015961
Eisosomes 0.000071 0.000082 0.000016 0.000045 0.000065 0.000069 0.000120 0.000044 0.000069 0.000074 0.000020 0.000045
Endoplasmic Reticulum 0.000963 0.002447 0.001406 0.002471 0.001161 0.001022 0.003585 0.001980 0.001038 0.001503 0.001487 0.002264
Endosome 0.004129 0.012744 0.000939 0.009329 0.005348 0.008373 0.005938 0.010854 0.004592 0.009850 0.001125 0.009971
Golgi 0.000395 0.002627 0.000567 0.002754 0.001277 0.002329 0.000682 0.004296 0.000730 0.002430 0.000572 0.003404
Lipid Particles 0.003546 0.006118 0.000238 0.001540 0.003507 0.004721 0.003332 0.002395 0.003531 0.005193 0.000353 0.001900
Mitochondria 0.001378 0.003965 0.002879 0.007526 0.001456 0.007850 0.000734 0.007812 0.001408 0.006537 0.002799 0.007646
Mitotic Spindle 0.002777 0.008252 0.023954 0.046997 0.001832 0.007368 0.001144 0.028753 0.002418 0.007667 0.023104 0.039314
None 0.023511 0.014064 0.012802 0.010775 0.017903 0.021689 0.013866 0.014518 0.021380 0.019113 0.012841 0.012351
Nuclear Periphery 0.000502 0.000752 0.001599 0.001668 0.000633 0.000710 0.001113 0.000993 0.000552 0.000724 0.001581 0.001384
Nuclear Periphery Foci 0.000349 0.000668 0.000154 0.000695 0.000612 0.000424 0.001155 0.000590 0.000449 0.000506 0.000191 0.000651
Nucleolus 0.003327 0.005849 0.001176 0.002286 0.004014 0.005909 0.002407 0.001589 0.003588 0.005888 0.001222 0.001992
Nucleus 0.456910* 0.433568* 0.627974* 0.390100* 0.465638* 0.509722* 0.682776* 0.398103* 0.460227* 0.483988* 0.630016* 0.393470*
Peroxisomes 0.001851 0.005516 0.000132 0.001986 0.002011 0.006548 0.000481 0.002610 0.001912 0.006199 0.000145 0.002249
Vacuole 0.030541 0.043219 0.014163 0.062502 0.028966 0.036257 0.039584 0.057357 0.029942 0.038609 0.015110 0.060335
Vacuole Periphery 0.000438 0.001435 0.001007 0.003775 0.000460 0.001409 0.003360 0.002571 0.000446 0.001418 0.001094 0.003268

Localization Changes (T-score)

R1 R2 R1 & R2
UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14 UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14 UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14
Actin -4.64 -12.77 -10.37 -4.15 -6.98 -2.86 -4.95 -5.94 -5.23 -1.91 -5.18 -14.51 -9.99 -7.42 -7.13
Bud Neck -5.10 -8.54 -2.18 3.54 6.12 -3.88 4.47 -0.00 4.76 -6.40 -6.36 -8.80 -1.66 5.32 7.84
Bud Site -5.72 -8.20 -8.97 -2.43 -4.09 -3.35 2.44 -7.44 -6.11 -8.33 -6.73 -8.22 -11.64 -7.14 -5.52
Cell Periphery 0.24 5.00 0.82 0.69 -1.36 -1.17 0.84 1.44 3.58 0.47 -0.05 5.61 1.25 1.36 -1.68
Cytoplasm 2.93 13.39 4.81 1.08 -9.95 4.93 9.17 3.21 -2.04 -7.69 6.31 16.41 5.75 -1.05 -12.76
Cytoplasmic Foci -0.87 18.92 16.51 14.34 -10.53 4.40 7.62 12.45 13.60 2.30 3.81 23.84 20.65 20.07 -13.01
Eisosomes -1.46 11.07 4.76 6.24 -16.94 -0.77 -3.28 3.72 5.37 4.30 -1.05 13.66 5.94 8.25 -17.75
Endoplasmic Reticulum -7.02 -6.80 -9.83 -2.87 -6.04 1.10 -5.87 -8.48 -11.14 3.18 -3.84 -8.06 -12.98 -10.48 -6.91
Endosome -5.27 8.50 -1.31 4.30 -6.29 -2.56 -0.07 -0.64 1.66 -0.26 -5.90 8.48 -1.40 4.22 -7.53
Golgi -2.84 0.17 -2.70 1.32 -2.50 -1.48 1.09 -1.12 0.12 -2.31 -3.79 1.21 -2.52 1.21 -3.50
Lipid Particles -2.00 6.80 4.32 4.04 -6.44 -1.58 0.14 2.70 3.79 2.36 -2.46 8.94 4.92 5.91 -4.65
Mitochondria -3.38 -4.51 -4.84 -1.06 -3.08 -5.09 1.62 -2.53 1.86 -3.20 -6.02 -4.28 -5.04 1.56 -3.26
Mitotic Spindle -2.39 -10.02 -11.16 -8.54 -4.48 -3.12 -0.96 -8.42 -6.28 -6.03 -3.64 -11.13 -13.89 -10.94 -4.25
None 3.69 5.46 6.48 3.21 3.54 -1.41 1.78 2.72 5.47 -0.14 1.03 5.93 6.82 6.80 2.43
Nuclear Periphery -2.73 -18.09 -13.76 -10.63 -0.11 -0.95 -4.98 -8.94 -7.80 1.14 -3.02 -18.36 -16.35 -13.30 2.65
Nuclear Periphery Foci -3.37 3.63 -3.86 -0.23 -6.36 0.73 -1.29 0.48 -0.53 1.35 -0.37 2.99 -2.33 -0.64 -5.63
Nucleolus -3.01 6.46 3.02 4.86 -1.68 -1.97 2.11 3.15 7.00 1.99 -3.69 6.14 4.50 7.83 -1.47
Nucleus 1.33 -17.37 -0.87 -2.22 20.74 -2.95 -8.49 0.09 3.82 9.10 -2.38 -20.81 -0.62 2.13 25.12
Peroxisomes -4.01 6.79 1.60 4.67 -3.43 -4.33 4.32 1.08 4.83 -2.88 -5.65 8.59 1.92 6.39 -5.04
Vacuole -2.67 4.01 -12.94 -9.06 -18.15 -0.96 -2.56 -11.36 -11.20 -3.33 -2.26 4.34 -17.19 -15.18 -22.51
Vacuole Periphery -6.84 -1.69 -3.75 -1.33 -2.91 -4.19 -0.89 -1.92 -0.03 0.40 -6.17 -1.89 -4.09 -0.99 -2.74
Standard name
Human Ortholog
Description Cyclin-dependent kinase inhibitor (CKI); inhibitor of Cdc28-Clb kinase complexes that controls G1/S phase transition, preventing premature S phase and ensuring genomic integrity; phosphorylated by Clb5/6-Cdk1 and Cln1/2-Cdk1 kinase which regulate timing of Sic1p degradation; phosphorylation targets Sic1p for SCF(CDC4)-dependent turnover; functional homolog of mammalian Kip1
Localization
Cell Percentages nucleus (45%), cytoplasm (10%), mixed (28%)
Cell Cycle Regulation Yes
cytoplasm - MA (6.1e-06)
Subcompartmental Group N/A

Sic1

Sic1


Nearest neighbours ( genes)

correlation threshold

0.6

1.0

0.80


Biological Process (Gene Ontology)
Molecular Function (Gene Ontology)
Cellular Component (Gene Ontology)

Endocytosis
Temp Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Vph1-GFP)
37℃
RT
Cell Cycle Omics

CYCLoPs (Sic1-GFP)
Gene / Allele Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Sac6-tdTomato)
Gene Images
Gene Images
Images are not yet available
Images are not yet available