Standard name
Human Ortholog
Description Protein of unknown function; overexpression suppresses the lethality caused by a rad53 null mutation
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Micrographs

ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 – – – – – –
Cell Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cytoplasm 0.17 0.26 0.21 0.15 0.19 0.15 0.1 0.09 0.05 0 0 0 0.29 0.39 0.5 0.52 0.53 0.57 0.13 0.12 0.09 0.21 0.18 0.21
Endoplasmic Reticulum 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0.1 0.07 0.06 0 0 0 0 0 0
Endosome 0 0 0.14 0 0.08 0 0 0 0 0 0 0 0 0.14 0.15 0.09 0.06 0.06 0 0 0 0 0 0
Golgi 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Mitochondria 0.71 0.25 0.25 0.41 0.31 0.66 0.75 0.73 0.71 0.75 0.86 0.81 0 0 0 0.05 0 0.06 0 0 0.09 0 0.05 0.08
Nucleus 0.28 0.35 0.3 0.45 0.43 0.37 0.36 0.45 0.54 0.53 0.52 0.51 0.63 0.33 0.22 0.15 0.1 0.06 0.43 0.43 0.34 0.38 0.3 0.22
Nuclear Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0.05 0.07 0.07 0.06 0.07 0.08
Vac/Vac Membrane 0.09 0.32 0.22 0.17 0.13 0.11 0.06 0 0 0.05 0 0 0.06 0.19 0.19 0.26 0.34 0.32 0.27 0.27 0.27 0.23 0.28 0.26
ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 2 0 1 2
Bud 1 0 1 4 1 2 12 5 9 11 7 12 0 0 0 0 0 1 1 2 3 5 9 9
Bud Neck 0 0 1 1 0 0 1 0 0 0 0 0 1 1 0 0 1 0 1 0 1 2 3 7
Bud Site 0 0 0 0 2 5 4 13 5 15 6 18 0 0 0 0 0 0 – – – – – –
Cell Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 2 0 0 0 0 0 0
Cytoplasm 50 27 66 35 54 58 56 51 24 26 11 20 60 162 191 115 120 117 46 15 41 82 90 115
Endoplasmic Reticulum 0 0 0 0 0 1 0 0 0 1 1 0 0 1 0 21 16 13 0 0 2 0 0 3
Endosome 0 0 44 10 22 13 8 4 1 2 0 0 5 59 58 19 13 13 2 2 17 7 18 17
Golgi 0 0 0 1 0 0 0 0 0 0 0 0 0 0 1 0 0 0 1 0 1 0 2 0
Mitochondria 212 26 78 95 89 264 402 393 346 455 357 442 1 2 3 10 5 12 13 4 41 11 22 46
Nucleus 84 37 91 105 124 146 192 242 265 320 214 280 130 134 84 32 22 12 153 53 151 149 152 123
Nuclear Periphery 0 0 0 1 0 1 3 1 2 1 3 9 0 0 3 0 0 0 0 0 3 1 2 1
Nucleolus 0 0 0 0 1 1 8 7 8 15 18 17 0 1 0 0 0 0 2 0 7 5 10 9
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0 2 0 0 1 0 0 0 0 0 0
SpindlePole 1 0 9 7 6 1 4 7 8 4 0 3 3 6 6 0 1 4 18 8 30 21 35 46
Vac/Vac Membrane 26 34 68 39 39 45 33 20 19 28 12 18 13 77 73 57 78 67 98 34 120 90 141 145
Unique Cell Count 299 105 307 234 289 399 538 540 490 608 415 549 206 412 381 220 227 207 357 125 441 389 506 559
Labelled Cell Count 374 124 358 298 338 537 723 743 687 878 629 819 213 443 421 254 256 242 357 125 441 389 506 559


Cytoplasm, Nucleus

(Huh et al. Nature 2003 425:686)
Screen WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 AF100 AF140 AF180
Mean Cell GFP Intensity (1e-4) 3.2 4.2 4.1 3.9 4.5 3.7 3.7 3.3 3.4 3.4 3.0 3.1 5.4 5.5 5.1 5.5 5.1 5.1 5.3 5.6 5.7
Std Deviation (1e-4) 0.3 0.5 1.3 1.6 3.0 1.8 1.7 1.1 1.0 0.9 0.9 0.7 1.1 1.0 0.9 1.3 1.1 1.7 1.0 1.5 1.5
Intensity Change (Log2) – – – -0.07 0.13 -0.17 -0.17 -0.3 -0.26 -0.3 -0.45 -0.4 0.39 0.4 0.3 0.41 0.3 0.31 0.37 0.43 0.47


Localization RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3
Actin – – – – – – – – – – – – – – –
Bud – – – – – – – – – – – – 0 0 0
Bud Neck – – – – – – – – – – – – 0 0 0
Bud Site – – – – – – – – – – – – 0 0 0
Cell Periphery – – – – – – – – – – – – 0 0 0
Cyto – – – – – – – – – – – – – – –
Endoplasmic Reticulum – – – – – – – – – – – – 0 0 0
Endosome – – – – – – – – – – – – 0 0 0
Golgi – – – – – – – – – – – – 0 0 0
Mitochondria – – – – – – – – – – – – 0 0 0
Nuclear Periphery – – – – – – – – – – – – 0 0 0
Nuc – – – – – – – – – – – – – – –
Nucleolus – – – – – – – – – – – – 0 0 0
Peroxisomes – – – – – – – – – – – – 0 0 0
SpindlePole – – – – – – – – – – – – 0 0 0
Vac – – – – – – – – – – – – – – –
Cortical Patches – – – – – – – – – – – – 0 0 0
Cytoplasm – – – – – – – – – – – – 0 0 0
Nucleus – – – – – – – – – – – – 0 0 0
Vacuole – – – – – – – – – – – – 1.0 3.1 2.6

Images

Circle plot not available

Protein Concentration and Protein Localization Data

R1 R2 R3
G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase
Concentration -0.9613 -0.4233 -0.6644 -0.7093 -1.1222 -0.4572 0.3025 0.3263 0.4882 0.3196 0.9425 0.4479 0.0397 -0.507 -0.5304 -0.1899 -0.7009 -0.2915
Actin 0.0015 0.0006 0.0004 0.0005 0.0002 0.0003 0.0203 0.0004 0.0068 0.0162 0.0325 0.0026 0.004 0.0052 0.015 0.0072 0.0001 0.0003
Bud 0.0003 0.0026 0.0001 0.0001 0 0.0002 0.0006 0.0002 0.0004 0.0003 0.0005 0.0001 0.0012 0.0003 0.0002 0.0001 0.0001 0.0001
Bud Neck 0.0022 0.0008 0.0003 0.0012 0.0009 0.0029 0.0089 0.0009 0.0033 0.002 0.0021 0.0023 0.0152 0.001 0.0005 0.0002 0.001 0.0021
Bud Periphery 0.0007 0.0006 0.0001 0.0001 0 0.0001 0.0009 0.0002 0.0006 0.0005 0.0008 0.0002 0.0019 0.0003 0.0002 0.0004 0.0001 0.0001
Bud Site 0.0015 0.0008 0.0002 0.0002 0.0001 0.0002 0.0033 0.0013 0.0114 0.0016 0.0014 0.0002 0.0156 0.0082 0.0012 0.0003 0.0005 0.0005
Cell Periphery 0.0001 0.0001 0 0 0 0 0.0002 0 0.0003 0.0001 0.0002 0.0001 0.0006 0.0001 0 0.0001 0.0001 0
Cytoplasm 0.055 0.0806 0.0405 0.0493 0.0254 0.0401 0.0459 0.0722 0.0405 0.027 0.0633 0.0434 0.0633 0.1001 0.0572 0.0218 0.0122 0.0461
Cytoplasmic Foci 0.0032 0.0032 0.0023 0.0024 0.0024 0.0014 0.0076 0.0037 0.0059 0.0057 0.0274 0.0026 0.0279 0.0062 0.003 0.0014 0.0032 0.004
Eisosomes 0.0002 0.0001 0.0001 0 0 0 0.0002 0 0.0001 0.0002 0.0005 0.0001 0.0002 0.0001 0.0001 0.0003 0 0
Endoplasmic Reticulum 0.0021 0.0034 0.0013 0.0025 0.0004 0.0008 0.0029 0.0021 0.004 0.0016 0.0028 0.0015 0.0059 0.0042 0.003 0.0013 0.0005 0.0017
Endosome 0.0039 0.0042 0.0014 0.0021 0.0015 0.0019 0.0093 0.0023 0.0072 0.0164 0.011 0.0033 0.0328 0.012 0.0098 0.0075 0.06 0.0148
Golgi 0.0005 0.0001 0.0001 0.0001 0.0001 0.0001 0.0023 0.0004 0.0027 0.004 0.005 0.0005 0.0064 0.0059 0.0035 0.0079 0.0056 0.0014
Lipid Particles 0.0007 0.0005 0.0003 0.0001 0.0006 0.0002 0.0086 0.0004 0.008 0.0143 0.0164 0.0012 0.0243 0.0019 0.005 0.0191 0.0019 0.0022
Mitochondria 0.0006 0.0016 0.0005 0.001 0.0004 0.0005 0.0034 0.0041 0.0027 0.004 0.0068 0.0055 0.0213 0.0069 0.0021 0.0413 0.0008 0.0008
None 0.1826 0.2345 0.1714 0.0663 0.1883 0.0803 0.1132 0.0775 0.0441 0.1433 0.1127 0.0643 0.0725 0.0928 0.0854 0.1055 0.0009 0.0433
Nuclear Periphery 0.0256 0.0366 0.0311 0.0256 0.0473 0.021 0.0242 0.0204 0.023 0.0278 0.0286 0.0157 0.058 0.0272 0.0332 0.013 0.0054 0.0126
Nucleolus 0.007 0.0102 0.0085 0.0106 0.0118 0.0179 0.0084 0.0062 0.0103 0.0078 0.0122 0.0113 0.0113 0.0044 0.0063 0.003 0.0134 0.0127
Nucleus 0.6411 0.5409 0.6622 0.735 0.6323 0.7497 0.6761 0.7487 0.7703 0.6559 0.5909 0.7676 0.5728 0.6951 0.7082 0.75 0.8755 0.8019
Peroxisomes 0.0004 0.0002 0.0002 0.0002 0.0001 0.0001 0.0053 0.002 0.0088 0.0071 0.0034 0.0003 0.0058 0.0023 0.0011 0.0026 0.0001 0.0006
Punctate Nuclear 0.069 0.0755 0.0783 0.1 0.0878 0.0814 0.0557 0.0553 0.0463 0.0611 0.0766 0.0757 0.0446 0.022 0.0614 0.0147 0.0137 0.0467
Vacuole 0.0014 0.0021 0.0005 0.002 0.0003 0.0008 0.002 0.0014 0.0025 0.002 0.0037 0.001 0.0096 0.0032 0.0024 0.0009 0.0042 0.0042
Vacuole Periphery 0.0004 0.0006 0.0003 0.0006 0.0003 0.0002 0.0007 0.0003 0.0007 0.0011 0.0012 0.0006 0.0048 0.0008 0.0012 0.0013 0.0008 0.004

Sequencing Data

R1 R2
G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Post-START S/G2 Metaphase Anaphase Telophase
Gene Expression 5.1473 1.6732 4.9665 5.8856 8.4939 7.4829 6.7673 4.8529 6.4074 8.8566
Translational Efficiency – – – – – – – – – –

Hit Data

Dataset Hit
Protein Concentration ✘
Protein Localization ✘
Gene Expression ✘
Translational Efficiency –

Micrographs

Cell Count

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
733 463 1648 2 1754 1589 8 539 2487 2052 1656 541

Protein Abundance

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
Mean 648.13 775.88 844.58 735.95 694.36 758.81 811.11 775.75 680.73 762.66 844.42 775.60
Standard Deviation 103.57 98.44 100.74 27.29 76.45 250.44 87.46 104.62 87.91 225.40 100.70 104.47
Intensity Change Log 2 — 0.259550 0.381951 0.183325 — 0.128055 0.224214 0.159908 — 0.193036 0.302521 0.171261

Localization Score (DeepLoc)

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
Actin 0.000090 0.000553 0.000330 0.000159 0.000055 0.000286 0.000231 0.000502 0.000066 0.000346 0.000329 0.000501
Bud Neck 0.003594 0.012036 0.004541 0.016071 0.001631 0.005180 0.000847 0.004262 0.002210 0.006727 0.004523 0.004306
Bud Site 0.000876 0.005602 0.003061 0.003844 0.000647 0.003546 0.000803 0.002566 0.000715 0.004010 0.003050 0.002571
Cell Periphery 0.000120 0.000163 0.000061 0.000581 0.000102 0.000154 0.000216 0.000111 0.000107 0.000156 0.000062 0.000112
Cytoplasm 0.242933* 0.167480* 0.030946 0.551045* 0.290012* 0.193231* 0.333287* 0.041862 0.276136* 0.187421* 0.032407 0.043744
Cytoplasmic Foci 0.079430 0.053226 0.000537 0.000446 0.054265 0.036322 0.006283 0.001980 0.061682 0.040136 0.000565 0.001974
Eisosomes 0.000072 0.000071 0.000017 0.000017 0.000044 0.000042 0.000017 0.000025 0.000052 0.000049 0.000017 0.000025
Endoplasmic Reticulum 0.000800 0.004775 0.003505 0.000992 0.000880 0.001799 0.001347 0.003967 0.000856 0.002471 0.003495 0.003956
Endosome 0.003114 0.004870 0.000464 0.001018 0.001525 0.002879 0.000553 0.004915 0.001993 0.003328 0.000464 0.004900
Golgi 0.001068 0.001378 0.000055 0.000078 0.000294 0.000549 0.000063 0.002975 0.000522 0.000736 0.000055 0.002964
Lipid Particles 0.008991 0.006731 0.000274 0.000114 0.005727 0.005455 0.000280 0.000495 0.006689 0.005743 0.000274 0.000494
Mitochondria 0.003159 0.010124 0.000988 0.000301 0.002974 0.006024 0.000439 0.006966 0.003028 0.006949 0.000985 0.006941
Mitotic Spindle 0.001399 0.014141 0.002812 0.004997 0.001887 0.004459 0.000472 0.003720 0.001744 0.006644 0.002800 0.003725
None 0.016275 0.010551 0.003582 0.029337 0.008018 0.008909 0.028697 0.008245 0.010452 0.009279 0.003703 0.008323
Nuclear Periphery 0.001536 0.002261 0.001032 0.000549 0.001366 0.002750 0.000341 0.000724 0.001416 0.002640 0.001028 0.000723
Nuclear Periphery Foci 0.000860 0.002045 0.000206 0.000259 0.000751 0.000757 0.000784 0.000387 0.000783 0.001047 0.000209 0.000386
Nucleolus 0.006426 0.008274 0.001247 0.000551 0.002416 0.005567 0.002536 0.003146 0.003598 0.006178 0.001253 0.003137
Nucleus 0.614804* 0.670660* 0.941188* 0.387432* 0.619860* 0.708604* 0.616000* 0.906415* 0.618370* 0.700043* 0.939617* 0.904496*
Peroxisomes 0.004139 0.007048 0.000161 0.000032 0.001323 0.002634 0.000092 0.000465 0.002153 0.003630 0.000161 0.000463
Vacuole 0.009919 0.016645 0.004659 0.002098 0.005950 0.010286 0.006637 0.005476 0.007120 0.011721 0.004668 0.005464
Vacuole Periphery 0.000394 0.001366 0.000338 0.000081 0.000273 0.000565 0.000074 0.000797 0.000309 0.000746 0.000336 0.000794

Localization Changes (T-score)

R1 R2 R1 & R2
UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14 UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14 UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14
Actin NaN NaN NaN NaN NaN NaN NaN NaN NaN NaN -2.27 -7.91 -5.35 -1.56 -1.25
Bud Neck NaN NaN NaN NaN NaN NaN NaN NaN NaN NaN -6.16 -6.13 -3.25 0.21 -0.58
Bud Site NaN NaN NaN NaN NaN NaN NaN NaN NaN NaN -4.41 -3.10 -3.47 0.78 -0.16
Cell Periphery NaN NaN NaN NaN NaN NaN NaN NaN NaN NaN -3.28 7.35 -0.20 2.47 -3.61
Cytoplasm NaN NaN NaN NaN NaN NaN NaN NaN NaN NaN 12.93 47.22 30.44 18.21 -3.63
Cytoplasmic Foci NaN NaN NaN NaN NaN NaN NaN NaN NaN NaN 6.73 26.78 25.63 17.95 -2.66
Eisosomes NaN NaN NaN NaN NaN NaN NaN NaN NaN NaN 0.50 13.44 4.78 4.16 -4.39
Endoplasmic Reticulum NaN NaN NaN NaN NaN NaN NaN NaN NaN NaN -9.29 -16.37 -9.54 -5.90 -1.70
Endosome NaN NaN NaN NaN NaN NaN NaN NaN NaN NaN -3.04 8.52 -1.21 -0.28 -2.32
Golgi NaN NaN NaN NaN NaN NaN NaN NaN NaN NaN -0.94 3.06 -1.46 -1.28 -1.88
Lipid Particles NaN NaN NaN NaN NaN NaN NaN NaN NaN NaN 1.47 14.74 14.05 12.80 -3.18
Mitochondria NaN NaN NaN NaN NaN NaN NaN NaN NaN NaN -3.45 3.01 -1.08 0.63 -2.01
Mitotic Spindle NaN NaN NaN NaN NaN NaN NaN NaN NaN NaN -3.52 -2.17 -2.24 -0.28 -1.09
None NaN NaN NaN NaN NaN NaN NaN NaN NaN NaN 1.10 7.99 0.98 0.17 -3.91
Nuclear Periphery NaN NaN NaN NaN NaN NaN NaN NaN NaN NaN -5.97 0.19 -1.05 3.34 -1.11
Nuclear Periphery Foci NaN NaN NaN NaN NaN NaN NaN NaN NaN NaN -1.52 3.15 0.55 2.19 -3.12
Nucleolus NaN NaN NaN NaN NaN NaN NaN NaN NaN NaN -4.39 4.80 0.94 4.44 -2.39
Nucleus NaN NaN NaN NaN NaN NaN NaN NaN NaN NaN -9.08 -47.01 -21.30 -14.01 6.38
Peroxisomes NaN NaN NaN NaN NaN NaN NaN NaN NaN NaN -3.32 6.96 6.12 8.70 -1.74
Vacuole NaN NaN NaN NaN NaN NaN NaN NaN NaN NaN -5.07 -0.56 -4.78 -3.63 -4.53
Vacuole Periphery NaN NaN NaN NaN NaN NaN NaN NaN NaN NaN -5.12 0.12 -1.17 0.40 -1.10
Standard name
Human Ortholog
Description Protein of unknown function; overexpression suppresses the lethality caused by a rad53 null mutation
Localization
Cell Percentages nucleus (48%)
Cell Cycle Regulation No
Subcompartmental Group nucleus-3

Srl2

Srl2


Nearest neighbours ( genes)

correlation threshold

0.6

1.0

0.80


Biological Process (Gene Ontology)
Molecular Function (Gene Ontology)
Cellular Component (Gene Ontology)

Endocytosis
Temp Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Vph1-GFP)
37℃
RT
Cell Cycle Omics

CYCLoPs (Srl2-GFP)
Gene / Allele Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Sac6-tdTomato)
Gene Images
Gene Images
Images are not yet available
Images are not yet available