Standard name
Human Ortholog
Description Subunit of the condensin complex; condensin reorganizes chromosomes during both mitosis and meiosis; forms a subcomplex with Smc2p that has ATP-hydrolyzing and DNA-binding activity, but other condensin subunits are required for chromatin binding; required for tRNA gene clustering at the nucleolus; potential Cdc28p substrate
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Micrographs

ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0.05 0 0 0.05 0 0 0 0 0 0 – – – – – –
Cell Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cytoplasm 0 0 0 0 0.05 0 0 0 0.05 0 0 0 0.09 0.16 0.18 0 0 0 0 0 0 0 0 0
Endoplasmic Reticulum 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Endosome 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0.07 0.06 0 0 0 0.06 0.12 0.12
Golgi 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Mitochondria 0.07 0.21 0.53 0.53 0.3 0.48 0.39 0.45 0.6 0.62 0.59 0.69 0 0.06 0.15 0.05 0 0.08 0 0 0 0 0 0
Nucleus 0.65 0.72 0.71 0.74 0.53 0.54 0.57 0.59 0.54 0.54 0.57 0.56 0.79 0.66 0.55 0.77 0.72 0.57 0.5 0.64 0.6 0.51 0.38 0.3
Nuclear Periphery 0 0 0.05 0 0.05 0 0 0 0 0 0.05 0 0 0 0 0 0 0.06 0.06 0 0 0 0 0
Nucleolus 0.32 0.17 0.18 0.2 0.34 0.25 0.28 0.22 0.23 0.23 0.23 0.2 0.12 0.16 0.17 0.17 0.18 0.3 0.25 0.15 0.21 0.27 0.19 0.17
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0.08 0 0.05 0 0.05 0 0 0 0 0 0 0 0 0.06 0.07 0 0 0.07 0.08 0.1
Vac/Vac Membrane 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0.07 0 0 0 0.06 0.16 0.22
ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 0 0 0 0 0 0 0 1 0 0 0 0 0 1 0 0 1 0
Bud 0 3 0 3 3 3 8 7 12 12 5 6 0 0 1 0 0 1 2 4 0 0 2 4
Bud Neck 0 0 0 0 0 1 1 0 0 2 0 1 0 0 0 0 0 0 1 2 0 0 1 1
Bud Site 1 1 0 0 6 5 20 24 20 21 11 31 0 2 2 0 0 0 – – – – – –
Cell Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cytoplasm 5 4 1 3 20 11 21 25 18 22 12 20 15 42 56 5 3 2 1 2 0 0 0 1
Endoplasmic Reticulum 0 0 0 0 0 0 0 0 0 0 0 0 0 1 1 4 0 3 0 0 0 0 0 0
Endosome 6 0 0 0 14 4 9 5 0 4 1 0 1 4 3 1 6 9 9 4 3 5 36 50
Golgi 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 1 1 2 0 0 0 1 0
Mitochondria 21 26 55 108 115 156 241 272 237 352 226 407 6 16 45 7 4 12 4 2 1 0 6 12
Nucleus 192 88 74 152 206 174 350 355 215 304 219 331 133 179 168 111 64 84 162 83 61 50 114 121
Nuclear Periphery 8 3 5 3 21 5 18 15 9 20 20 17 1 2 8 3 1 9 18 1 3 0 2 4
Nucleolus 94 21 19 40 132 82 175 132 90 130 89 118 20 43 52 24 16 44 79 19 21 26 55 70
Peroxisomes 0 1 0 0 0 0 2 0 1 0 0 0 0 0 0 1 1 0 2 0 0 0 0 1
SpindlePole 8 0 1 5 33 14 30 24 19 7 8 11 0 4 4 2 3 9 21 2 4 6 25 39
Vac/Vac Membrane 4 3 0 0 3 1 12 4 4 3 2 7 1 10 13 2 4 10 9 4 2 6 47 88
Unique Cell Count 296 123 104 205 389 323 618 603 396 568 383 594 169 270 305 144 89 148 323 131 103 99 301 406
Labelled Cell Count 339 150 155 314 553 456 887 863 625 877 593 949 177 304 353 160 103 184 323 131 103 99 301 406


Nucleus

(Huh et al. Nature 2003 425:686)
Screen WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 AF100 AF140 AF180
Mean Cell GFP Intensity (1e-4) 6.8 5.5 4.3 4.5 4.8 4.5 4.2 4.3 3.8 3.9 3.9 3.5 6.6 6.0 5.5 8.1 9.4 9.6 6.0 6.4 6.8
Std Deviation (1e-4) 1.2 0.9 0.5 1.4 1.2 1.3 1.1 1.2 1.1 1.2 1.3 1.0 1.3 1.4 1.4 1.4 1.8 2.0 1.0 1.4 1.4
Intensity Change (Log2) – – – 0.09 0.18 0.08 -0.01 0.02 -0.18 -0.13 -0.12 -0.3 0.63 0.49 0.36 0.93 1.15 1.17 0.5 0.59 0.68


Localization RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3
Cortical Patches 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cell Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cytoplasm 0 0 0 0 0 0 0 0 0 2.7 4.0 4.4 0 0 0
Endoplasmic Reticulum 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Endosome 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Golgi 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Mitochondria 0 0 0 0 0 0 0 0 0 -9.5 -10.4 -7.8 -8.6 -7.3 -7.9
Nucleus 0.6 -3.3 -3.1 -2.8 -2.4 -3.1 -3.3 -2.6 -2.9 1.4 -0.9 -2.9 1.1 0.1 -2.3
Nuclear Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0.4
Nucleolus 0 0 0 0 0 0 0 0 0 -1.5 -0.5 -0.3 -0.3 -0.1 2.1
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Vacuole 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0

Images

Circle plot not available

Protein Concentration and Protein Localization Data

R1 R2 R3
G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase
Concentration 3.6784 3.6226 3.6316 3.3451 3.1934 3.644 3.0169 3.0756 2.9482 2.8357 2.621 2.9422 1.1884 0.6061 0.7742 1.0946 0.9036 0.8387
Actin 0.0217 0.0003 0.0006 0 0.0032 0.0001 0.0251 0.001 0.0129 0.0003 0.0009 0.0001 0.0003 0.0004 0.0084 0.0654 0.0197 0.0002
Bud 0.0003 0.0001 0.0002 0.0001 0.0005 0.0001 0.0003 0.0001 0.0009 0.0004 0.001 0.0001 0.0001 0.0001 0.0002 0.0006 0.0004 0.0001
Bud Neck 0.0016 0.0015 0.0023 0.0018 0.0297 0.0031 0.0011 0.0003 0.0016 0.0032 0.0045 0.0026 0.0055 0.0003 0.0035 0.0015 0.0101 0.0029
Bud Periphery 0.0006 0.0002 0.0008 0.0002 0.0022 0.0004 0.0003 0.0002 0.0015 0.001 0.0083 0.0003 0.0005 0.0002 0.0005 0.0008 0.0015 0.0002
Bud Site 0.0016 0.0036 0.0009 0.0007 0.0017 0.0004 0.0064 0.0005 0.0056 0.0022 0.0017 0.0002 0.001 0.0003 0.0033 0.0032 0.0014 0.0002
Cell Periphery 0.0002 0.0001 0.0004 0.0002 0.0016 0.0001 0.0001 0.0001 0.0003 0.0003 0.0007 0.0001 0.0003 0.0001 0.0003 0.0002 0.0004 0.0003
Cytoplasm 0.0027 0.003 0.0017 0.0001 0.0003 0.0003 0.0028 0.0023 0.001 0.0008 0.0002 0.0002 0.0044 0.0304 0.0023 0.0021 0.0011 0.0017
Cytoplasmic Foci 0.0075 0.0032 0.001 0.0006 0.0057 0.0003 0.0052 0.0003 0.0017 0.0019 0.0028 0.0001 0.0085 0.0001 0.0076 0.0134 0.0092 0.0004
Eisosomes 0.0003 0.0001 0.0001 0 0.0002 0 0.0002 0 0.0001 0 0.0001 0 0.0003 0 0.0001 0.0006 0.0009 0
Endoplasmic Reticulum 0.0016 0.0006 0.0016 0 0.0005 0.0004 0.0009 0.0003 0.0006 0.0002 0.0002 0.0001 0.0007 0.0007 0.0006 0.0007 0.0014 0.0001
Endosome 0.0208 0.0007 0.0097 0.0003 0.0178 0.0025 0.0093 0.0023 0.0109 0.0016 0.0023 0.0002 0.0032 0.0005 0.0053 0.0101 0.0118 0.001
Golgi 0.0064 0.0002 0.001 0.0001 0.0124 0.0002 0.0013 0.001 0.0017 0.0007 0.0011 0.0001 0.0004 0 0.0025 0.0138 0.0054 0.0002
Lipid Particles 0.0236 0.0006 0.0061 0.0019 0.0407 0.0013 0.0032 0.0004 0.0027 0.0071 0.0062 0.0003 0.004 0.0001 0.0102 0.0125 0.0548 0.0016
Mitochondria 0.0089 0.0004 0.0037 0.0002 0.0772 0.0003 0.0016 0.0057 0.0132 0.0009 0.0218 0.0003 0.0008 0.0009 0.0036 0.0208 0.0159 0.0002
None 0.004 0.0058 0.003 0.0001 0.0004 0.0001 0.0025 0.0002 0.0006 0.0006 0.0008 0.0001 0.0229 0.001 0.0024 0.0027 0.0025 0.004
Nuclear Periphery 0.0404 0.0462 0.0696 0.0009 0.003 0.0097 0.0157 0.0202 0.0244 0.0216 0.004 0.0185 0.0399 0.0804 0.0402 0.0097 0.0553 0.0129
Nucleolus 0.0789 0.0751 0.2373 0.459 0.4835 0.1696 0.0393 0.0529 0.248 0.5201 0.4903 0.1321 0.0854 0.0364 0.1441 0.3357 0.2973 0.127
Nucleus 0.7211 0.7721 0.5827 0.4672 0.1699 0.766 0.8448 0.8905 0.6274 0.3111 0.21 0.8286 0.752 0.8417 0.631 0.3837 0.1594 0.7477
Peroxisomes 0.0052 0.0003 0.0007 0.0001 0.0039 0.0001 0.0018 0.0001 0.0007 0.0005 0.0014 0.0001 0.0004 0 0.0158 0.0145 0.0044 0.0001
Punctate Nuclear 0.0445 0.0847 0.0701 0.0659 0.1222 0.0437 0.0366 0.019 0.035 0.1228 0.2333 0.0156 0.0673 0.004 0.1141 0.1067 0.3419 0.0966
Vacuole 0.0055 0.0008 0.0043 0.0006 0.0134 0.0008 0.0007 0.0017 0.0048 0.0018 0.0048 0.0003 0.0015 0.0007 0.0027 0.0008 0.0022 0.0023
Vacuole Periphery 0.0025 0.0005 0.0024 0.0001 0.0098 0.0003 0.0007 0.0008 0.0042 0.001 0.0035 0.0002 0.0006 0.0018 0.0014 0.0004 0.003 0.0004

Sequencing Data

R1 R2
G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Post-START S/G2 Metaphase Anaphase Telophase
Gene Expression 15.4223 18.7683 17.7095 18.2101 18.1473 15.2842 24.8921 24.9043 21.5013 20.0916
Translational Efficiency 0.9892 0.7123 0.7112 0.7989 0.6993 0.7294 0.6356 0.5341 0.5603 0.5812

Hit Data

Dataset Hit
Protein Concentration ✘
Protein Localization ✔
Gene Expression ✘
Translational Efficiency ✘
Standard name
Human Ortholog
Description Subunit of the condensin complex; condensin reorganizes chromosomes during both mitosis and meiosis; forms a subcomplex with Smc2p that has ATP-hydrolyzing and DNA-binding activity, but other condensin subunits are required for chromatin binding; required for tRNA gene clustering at the nucleolus; potential Cdc28p substrate
Localization
Cell Percentages nucleus (60%), nucleolus (9%), mixed (26%)
Cell Cycle Regulation Yes
nucleolus - S/G2 (0.0001)
nucleolus - MA (0.00017)
nucleus - T/G1 (8.8e-09)
Subcompartmental Group N/A

Smc4

Smc4


Nearest neighbours ( genes)

correlation threshold

0.6

1.0

0.80


Biological Process (Gene Ontology)
Molecular Function (Gene Ontology)
Cellular Component (Gene Ontology)

Endocytosis
Temp Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Vph1-GFP)
37℃
RT
Cell Cycle Omics

CYCLoPs (Smc4-GFP)
Gene / Allele Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Sac6-tdTomato)
Gene Images
Gene Images
Images are not yet available
Images are not yet available