Standard name | |
---|---|
Human Ortholog | |
Description | v-SNARE component of the vacuolar SNARE complex; involved in vesicle fusion; inhibits ATP-dependent Ca(2+) transport activity of Pmc1p in the vacuolar membrane |
ensLOC | DeepLoc | |||||||||||||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Localization | WT1 | WT2 | WT3 | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 | WT1 | WT2 | WT3 | AF100 | AF140 | AF180 |
Cortical Patches | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 2 | 0 | 0 | 0 | 3 |
Bud | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 2 | 1 | 1 | 0 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 3 | 3 | 1 | 1 | 0 | 2 |
Bud Neck | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 0 | 0 | 0 | 0 | 1 |
Bud Site | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | – | – | – | – | – | – |
Cell Periphery | 0 | 2 | 0 | 0 | 0 | 0 | 0 | 1 | 0 | 0 | 0 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Cytoplasm | 98 | 151 | 46 | 72 | 79 | 105 | 179 | 216 | 121 | 168 | 120 | 255 | 157 | 221 | 225 | 24 | 16 | 9 | 8 | 14 | 4 | 10 | 8 | 7 |
Endoplasmic Reticulum | 1 | 3 | 1 | 2 | 0 | 3 | 0 | 2 | 0 | 1 | 0 | 0 | 1 | 1 | 1 | 4 | 3 | 2 | 0 | 1 | 1 | 0 | 0 | 2 |
Endosome | 13 | 6 | 1 | 0 | 3 | 2 | 0 | 1 | 0 | 0 | 0 | 1 | 1 | 1 | 1 | 0 | 0 | 1 | 3 | 5 | 5 | 2 | 5 | 7 |
Golgi | 0 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 2 | 0 | 0 | 0 | 1 |
Mitochondria | 10 | 11 | 2 | 0 | 2 | 3 | 4 | 2 | 12 | 11 | 16 | 20 | 0 | 0 | 1 | 0 | 0 | 2 | 5 | 8 | 0 | 5 | 4 | 4 |
Nucleus | 60 | 46 | 52 | 25 | 71 | 52 | 79 | 42 | 11 | 14 | 13 | 19 | 3 | 2 | 2 | 30 | 13 | 18 | 1 | 3 | 0 | 4 | 3 | 1 |
Nuclear Periphery | 0 | 1 | 0 | 1 | 1 | 3 | 1 | 1 | 3 | 2 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 2 | 0 | 0 | 4 |
Nucleolus | 0 | 0 | 1 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 0 | 0 | 0 | 0 | 1 | 0 | 0 | 2 | 0 | 0 | 0 | 0 | 0 |
Peroxisomes | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 0 | 0 | 0 | 0 | 1 | 0 | 0 | 0 | 0 |
SpindlePole | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 0 | 0 | 1 | 0 | 0 | 0 | 2 | 1 | 0 | 1 | 2 | 1 |
Vac/Vac Membrane | 232 | 257 | 81 | 71 | 144 | 169 | 177 | 153 | 79 | 90 | 62 | 108 | 51 | 25 | 9 | 41 | 40 | 37 | 336 | 379 | 150 | 131 | 149 | 183 |
Unique Cell Count | 338 | 389 | 151 | 132 | 220 | 242 | 328 | 330 | 168 | 235 | 168 | 336 | 194 | 239 | 233 | 74 | 50 | 58 | 368 | 429 | 168 | 162 | 179 | 226 |
Labelled Cell Count | 414 | 478 | 184 | 172 | 300 | 337 | 440 | 422 | 227 | 287 | 213 | 406 | 213 | 250 | 241 | 100 | 72 | 69 | 368 | 429 | 168 | 162 | 179 | 226 |
Screen | WT1 | WT2 | WT3 | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 | AF100 | AF140 | AF180 |
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Mean Cell GFP Intensity (1e-4) | 5.2 | 5.2 | 5.8 | 5.5 | 6.7 | 5.9 | 5.5 | 6.0 | 4.6 | 5.1 | 4.9 | 5.1 | 5.2 | 5.1 | 4.9 | 5.4 | 6.2 | 7.0 | 6.1 | 6.4 | 6.3 |
Std Deviation (1e-4) | 0.9 | 1.3 | 1.1 | 1.0 | 1.2 | 1.4 | 1.3 | 1.6 | 0.9 | 1.0 | 1.3 | 1.1 | 1.1 | 1.0 | 1.7 | 1.4 | 1.4 | 2.4 | 1.7 | 1.8 | 1.7 |
Intensity Change (Log2) | – | – | – | -0.08 | 0.21 | 0.03 | -0.08 | 0.04 | -0.32 | -0.2 | -0.25 | -0.2 | -0.16 | -0.18 | -0.23 | -0.11 | 0.09 | 0.28 | 0.07 | 0.13 | 0.12 |
Localization | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 |
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Cortical Patches | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Bud | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Bud Neck | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Bud Site | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Cell Periphery | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Cytoplasm | 4.1 | 1.1 | 2.6 | 4.9 | 7.2 | 7.4 | 7.9 | 7.3 | 9.5 | 9.4 | 12.8 | 13.9 | 0.3 | 0.2 | -2.2 |
Endoplasmic Reticulum | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Endosome | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Golgi | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Mitochondria | 0 | 0 | 0 | 0 | 0 | 0 | 1.8 | 0 | 2.3 | 0 | 0 | 0 | 0 | 0 | 0 |
Nucleus | -2.9 | -0.4 | -2.8 | -2.4 | -5.6 | -6.2 | -7.3 | -5.9 | -8.3 | -8.3 | -9.4 | -9.2 | 0.9 | -1.1 | -0.5 |
Nuclear Periphery | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Nucleolus | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Peroxisomes | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
SpindlePole | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Vacuole | 0 | 2.3 | 3.2 | 0.1 | -1.5 | -1.2 | -3.0 | -3.0 | -4.5 | -5.2 | -9.3 | -11.2 | 0.2 | 3.3 | 1.3 |
R1 | R2 | R3 | ||||||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | |
Concentration | 1.6378 | 2.631 | 1.8216 | 1.401 | 1.5002 | 2.3614 | 4.3548 | 4.959 | 4.4049 | 3.8775 | 3.2875 | 4.3422 | 0.942 | 2.4607 | 1.6373 | 1.8921 | 1.563 | 2.109 |
Actin | 0.0267 | 0 | 0.015 | 0 | 0.0002 | 0.0012 | 0.0351 | 0.0001 | 0.0048 | 0 | 0 | 0 | 0.0052 | 0 | 0.0026 | 0.0002 | 0.0088 | 0.0002 |
Bud | 0.0008 | 0.0004 | 0.0005 | 0.0008 | 0.0004 | 0.0005 | 0.0007 | 0.0004 | 0.0009 | 0.0006 | 0.0005 | 0.0002 | 0.0003 | 0.0005 | 0.0007 | 0.0004 | 0.001 | 0.0003 |
Bud Neck | 0.0017 | 0.0015 | 0.0018 | 0.0012 | 0.001 | 0.0029 | 0.0009 | 0.0007 | 0.0012 | 0.0003 | 0.0025 | 0.002 | 0.0008 | 0.0015 | 0.0021 | 0.0009 | 0.0012 | 0.0019 |
Bud Periphery | 0.0019 | 0.0008 | 0.0016 | 0.002 | 0.001 | 0.0016 | 0.001 | 0.0009 | 0.0017 | 0.0023 | 0.0014 | 0.0009 | 0.0007 | 0.001 | 0.0024 | 0.0009 | 0.0023 | 0.0009 |
Bud Site | 0.006 | 0.0026 | 0.0029 | 0.0028 | 0.0008 | 0.0013 | 0.0019 | 0.0013 | 0.0057 | 0.0006 | 0.0038 | 0.0005 | 0.0009 | 0.0019 | 0.0041 | 0.0015 | 0.0019 | 0.0007 |
Cell Periphery | 0.0029 | 0.0022 | 0.0026 | 0.0029 | 0.0019 | 0.0031 | 0.0017 | 0.0025 | 0.0039 | 0.0008 | 0.0034 | 0.0019 | 0.001 | 0.0016 | 0.0022 | 0.0007 | 0.0012 | 0.0017 |
Cytoplasm | 0.0246 | 0.0602 | 0.0288 | 0.016 | 0.0082 | 0.011 | 0.0284 | 0.026 | 0.0181 | 0.0025 | 0.0025 | 0.007 | 0.0196 | 0.0293 | 0.0182 | 0.0207 | 0.0048 | 0.0141 |
Cytoplasmic Foci | 0.0423 | 0.0259 | 0.0259 | 0.0078 | 0.0156 | 0.0089 | 0.0213 | 0.0249 | 0.0134 | 0.0131 | 0.0027 | 0.0054 | 0.0285 | 0.0147 | 0.015 | 0.0196 | 0.018 | 0.015 |
Eisosomes | 0.001 | 0 | 0.0006 | 0 | 0 | 0 | 0.0002 | 0 | 0 | 0 | 0 | 0 | 0.0002 | 0 | 0.0001 | 0 | 0.0001 | 0 |
Endoplasmic Reticulum | 0.0059 | 0.0056 | 0.0081 | 0.0025 | 0.0011 | 0.0029 | 0.014 | 0.0036 | 0.0079 | 0.0008 | 0.0009 | 0.0022 | 0.0056 | 0.0055 | 0.0053 | 0.0018 | 0.006 | 0.004 |
Endosome | 0.2494 | 0.1698 | 0.2592 | 0.221 | 0.1785 | 0.1753 | 0.2721 | 0.1186 | 0.1892 | 0.3458 | 0.1218 | 0.1614 | 0.3028 | 0.1916 | 0.269 | 0.259 | 0.2839 | 0.2119 |
Golgi | 0.0197 | 0.0027 | 0.0099 | 0.003 | 0.0038 | 0.0108 | 0.0115 | 0.0045 | 0.006 | 0.0102 | 0.0013 | 0.0031 | 0.0076 | 0.0031 | 0.0061 | 0.0095 | 0.0107 | 0.0035 |
Lipid Particles | 0.0556 | 0.0177 | 0.0338 | 0.0059 | 0.0068 | 0.0121 | 0.0165 | 0.0225 | 0.026 | 0.0063 | 0.0089 | 0.0119 | 0.0249 | 0.0069 | 0.0202 | 0.0047 | 0.0183 | 0.0135 |
Mitochondria | 0.0294 | 0.0046 | 0.0249 | 0.0032 | 0.0475 | 0.0268 | 0.0061 | 0.0093 | 0.038 | 0.0425 | 0.0005 | 0.0056 | 0.016 | 0.016 | 0.0357 | 0.0231 | 0.0214 | 0.0091 |
None | 0.0652 | 0.0759 | 0.033 | 0.0038 | 0.006 | 0.0039 | 0.0338 | 0.0141 | 0.0412 | 0.0015 | 0.001 | 0.0089 | 0.0543 | 0.0366 | 0.0281 | 0.0081 | 0.003 | 0.012 |
Nuclear Periphery | 0.0105 | 0.0073 | 0.0148 | 0.0024 | 0.0023 | 0.004 | 0.0598 | 0.0083 | 0.0125 | 0.0014 | 0.0017 | 0.0058 | 0.0155 | 0.0069 | 0.0099 | 0.0037 | 0.008 | 0.0111 |
Nucleolus | 0.0121 | 0.0044 | 0.0063 | 0.004 | 0.0043 | 0.0027 | 0.0028 | 0.0033 | 0.006 | 0.0014 | 0.0059 | 0.0047 | 0.0027 | 0.0038 | 0.0083 | 0.0034 | 0.003 | 0.0033 |
Nucleus | 0.012 | 0.0094 | 0.0086 | 0.0051 | 0.0059 | 0.0052 | 0.015 | 0.008 | 0.0057 | 0.0015 | 0.0051 | 0.0058 | 0.0055 | 0.0076 | 0.0106 | 0.0072 | 0.0044 | 0.0103 |
Peroxisomes | 0.0366 | 0.0009 | 0.0037 | 0.0008 | 0.0006 | 0.001 | 0.0014 | 0.0014 | 0.0034 | 0.0011 | 0.0006 | 0.0013 | 0.0029 | 0.0006 | 0.0034 | 0.0007 | 0.0086 | 0.0011 |
Punctate Nuclear | 0.0036 | 0.0016 | 0.0012 | 0.0002 | 0.0011 | 0.0003 | 0.0086 | 0.0009 | 0.0016 | 0.0001 | 0.0002 | 0.0003 | 0.0038 | 0.0004 | 0.0015 | 0.0012 | 0.0088 | 0.0021 |
Vacuole | 0.3425 | 0.5547 | 0.4348 | 0.6378 | 0.624 | 0.6397 | 0.4007 | 0.5768 | 0.5279 | 0.4531 | 0.7587 | 0.6464 | 0.3983 | 0.5623 | 0.4556 | 0.555 | 0.5052 | 0.5823 |
Vacuole Periphery | 0.0498 | 0.0516 | 0.082 | 0.0769 | 0.0888 | 0.0847 | 0.0665 | 0.1722 | 0.0848 | 0.1139 | 0.0765 | 0.1245 | 0.1028 | 0.1083 | 0.099 | 0.0777 | 0.0795 | 0.1011 |
R1 | R2 | |||||||||
---|---|---|---|---|---|---|---|---|---|---|
G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | |
Gene Expression | 39.821 | 29.7725 | 30.7374 | 44.4919 | 33.794 | 28.9447 | 46.4633 | 50.352 | 47.9555 | 46.8068 |
Translational Efficiency | 1.3797 | 1.6798 | 1.7368 | 1.2005 | 1.8466 | 2.5436 | 1.2858 | 1.6625 | 1.4977 | 1.5111 |
Dataset | Hit |
---|---|
Protein Concentration | ✘ |
Protein Localization | ✘ |
Gene Expression | ✘ |
Translational Efficiency | ✘ |
R1 | R2 | R1 & R2 | |||||||||
---|---|---|---|---|---|---|---|---|---|---|---|
WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 |
2161 | 1661 | 2106 | 1222 | 694 | 1982 | 2372 | 1718 | 2855 | 3643 | 4478 | 2940 |
R1 | R2 | R1 & R2 | ||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|
WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 | |
Mean | 720.11 | 693.95 | 797.36 | 745.02 | 710.84 | 842.96 | 811.66 | 848.61 | 717.86 | 775.02 | 804.93 | 805.55 |
Standard Deviation | 92.40 | 105.97 | 90.36 | 115.57 | 76.51 | 103.31 | 87.66 | 121.34 | 88.88 | 128.20 | 89.23 | 129.47 |
Intensity Change Log 2 | — | -0.053386 | 0.147014 | 0.049062 | — | 0.245939 | 0.191351 | 0.255577 | — | 0.103059 | 0.169209 | 0.155343 |
R1 | R2 | R1 & R2 | ||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|
WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 | |
Actin | 0.000055 | 0.000095 | 0.000090 | 0.001261 | 0.000071 | 0.000067 | 0.000095 | 0.000355 | 0.000059 | 0.000080 | 0.000093 | 0.000731 |
Bud Neck | 0.016007 | 0.017970 | 0.005751 | 0.008992 | 0.015963 | 0.026562 | 0.004804 | 0.013054 | 0.015997 | 0.022645 | 0.005249 | 0.011366 |
Bud Site | 0.004409 | 0.011626 | 0.011947 | 0.011956 | 0.004264 | 0.015309 | 0.009745 | 0.044290 | 0.004374 | 0.013630 | 0.010781 | 0.030851 |
Cell Periphery | 0.000062 | 0.000098 | 0.000090 | 0.000713 | 0.000102 | 0.000153 | 0.000109 | 0.000117 | 0.000072 | 0.000128 | 0.000100 | 0.000365 |
Cytoplasm | 0.296817* | 0.374519* | 0.457723* | 0.378904* | 0.313567* | 0.176174* | 0.535703* | 0.485850* | 0.300889* | 0.266608* | 0.499029* | 0.441398* |
Cytoplasmic Foci | 0.065132 | 0.065971 | 0.007212 | 0.023143 | 0.039143 | 0.044265 | 0.013064 | 0.022001 | 0.058815 | 0.054162 | 0.010312 | 0.022476 |
Eisosomes | 0.000042 | 0.000034 | 0.000018 | 0.000100 | 0.000044 | 0.000041 | 0.000024 | 0.000029 | 0.000042 | 0.000038 | 0.000021 | 0.000059 |
Endoplasmic Reticulum | 0.000994 | 0.000950 | 0.001408 | 0.001649 | 0.001447 | 0.001722 | 0.001111 | 0.001601 | 0.001104 | 0.001370 | 0.001250 | 0.001621 |
Endosome | 0.039582 | 0.029982 | 0.001438 | 0.009204 | 0.023300 | 0.039821 | 0.002192 | 0.011594 | 0.035624 | 0.035335 | 0.001837 | 0.010601 |
Golgi | 0.000867 | 0.001481 | 0.000222 | 0.002247 | 0.000370 | 0.001770 | 0.000160 | 0.007202 | 0.000747 | 0.001638 | 0.000189 | 0.005142 |
Lipid Particles | 0.002860 | 0.002139 | 0.000585 | 0.002197 | 0.002006 | 0.001713 | 0.000644 | 0.000894 | 0.002653 | 0.001907 | 0.000617 | 0.001435 |
Mitochondria | 0.001236 | 0.003151 | 0.000690 | 0.002113 | 0.000772 | 0.003142 | 0.000368 | 0.001967 | 0.001123 | 0.003146 | 0.000520 | 0.002028 |
Mitotic Spindle | 0.002197 | 0.003428 | 0.002943 | 0.011934 | 0.000363 | 0.004944 | 0.005180 | 0.024102 | 0.001751 | 0.004253 | 0.004128 | 0.019044 |
None | 0.000613 | 0.003003 | 0.004161 | 0.009483 | 0.000432 | 0.000578 | 0.003697 | 0.002605 | 0.000569 | 0.001683 | 0.003915 | 0.005464 |
Nuclear Periphery | 0.000414 | 0.000464 | 0.002226 | 0.001374 | 0.000279 | 0.000338 | 0.001387 | 0.001225 | 0.000381 | 0.000396 | 0.001781 | 0.001286 |
Nuclear Periphery Foci | 0.000164 | 0.000260 | 0.000343 | 0.001465 | 0.000075 | 0.000179 | 0.000472 | 0.000784 | 0.000142 | 0.000216 | 0.000412 | 0.001067 |
Nucleolus | 0.000968 | 0.001976 | 0.000468 | 0.001960 | 0.000708 | 0.000835 | 0.000507 | 0.000807 | 0.000905 | 0.001355 | 0.000489 | 0.001286 |
Nucleus | 0.051905 | 0.075417 | 0.460838* | 0.314000* | 0.062214 | 0.036675 | 0.318479* | 0.128303 | 0.054411 | 0.054339 | 0.385431* | 0.205487* |
Peroxisomes | 0.001640 | 0.002955 | 0.000125 | 0.001600 | 0.000650 | 0.002445 | 0.000264 | 0.000948 | 0.001399 | 0.002678 | 0.000199 | 0.001219 |
Vacuole | 0.511041* | 0.399645* | 0.041356 | 0.213263* | 0.531288* | 0.635888* | 0.101507 | 0.249325* | 0.515962* | 0.528175* | 0.073218 | 0.234336* |
Vacuole Periphery | 0.002994 | 0.004836 | 0.000365 | 0.002443 | 0.002945 | 0.007377 | 0.000489 | 0.002949 | 0.002982 | 0.006219 | 0.000431 | 0.002738 |
R1 | R2 | R1 & R2 | |||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
UBP2_WT | UBP14_WT | UBP2UBP14_WT | UBP2UBP14_UBP2 | UBP2UBP14_UBP14 | UBP2_WT | UBP14_WT | UBP2UBP14_WT | UBP2UBP14_UBP2 | UBP2UBP14_UBP14 | UBP2_WT | UBP14_WT | UBP2UBP14_WT | UBP2UBP14_UBP2 | UBP2UBP14_UBP14 | |
Actin | -1.68 | -3.43 | -1.46 | -1.39 | -1.37 | 0.08 | -0.83 | -3.56 | -5.32 | -3.48 | -1.26 | -2.60 | -1.93 | -1.84 | -1.72 |
Bud Neck | -1.27 | 9.88 | 7.07 | 6.74 | -2.59 | -4.98 | 7.15 | 2.85 | 8.77 | -6.41 | -5.30 | 12.92 | 6.48 | 10.86 | -6.63 |
Bud Site | -5.92 | -3.21 | -4.28 | -0.16 | -1.27 | -7.44 | -2.37 | -8.89 | -4.10 | -7.13 | -10.09 | -4.09 | -10.10 | -3.78 | -6.72 |
Cell Periphery | -2.74 | -2.54 | -2.87 | -2.64 | -2.74 | -4.34 | 0.61 | 1.93 | 6.40 | 0.26 | -6.83 | -1.23 | -2.91 | -2.01 | -2.65 |
Cytoplasm | -8.32 | -12.73 | -6.46 | 1.41 | 4.32 | 11.99 | -14.96 | -11.22 | -31.64 | 3.94 | 4.98 | -22.42 | -15.36 | -20.72 | 5.04 |
Cytoplasmic Foci | -0.27 | 27.04 | 18.70 | 16.71 | -9.76 | -1.83 | 11.55 | 9.00 | 13.00 | -4.78 | 1.99 | 28.49 | 22.17 | 21.11 | -10.35 |
Eisosomes | 2.55 | 6.56 | -7.99 | -10.56 | -13.40 | 0.33 | 2.58 | 2.19 | 5.78 | -1.99 | 1.48 | 6.53 | -2.68 | -5.59 | -12.84 |
Endoplasmic Reticulum | 0.42 | -7.41 | -5.13 | -6.13 | 1.99 | -1.39 | 0.03 | -0.59 | 0.91 | -0.88 | -2.36 | -6.28 | -4.58 | -2.46 | 0.94 |
Endosome | 4.80 | 26.22 | 19.16 | 12.91 | -6.50 | -8.35 | 15.43 | 10.82 | 20.73 | -6.28 | 0.20 | 29.30 | 22.53 | 24.04 | -9.06 |
Golgi | -1.24 | 2.77 | -0.93 | -0.05 | -2.10 | -3.30 | 2.64 | -3.26 | -1.48 | -3.56 | -2.57 | 3.25 | -2.93 | -1.35 | -4.12 |
Lipid Particles | 2.49 | 10.43 | 3.00 | 0.52 | -5.33 | 1.15 | 8.28 | 6.30 | 3.69 | -6.62 | 3.52 | 12.22 | 6.80 | 2.88 | -6.89 |
Mitochondria | -2.22 | 2.22 | -0.77 | 1.61 | -2.11 | -5.17 | 4.99 | -3.04 | 3.78 | -5.37 | -4.24 | 3.18 | -1.20 | 3.48 | -4.38 |
Mitotic Spindle | -0.83 | -0.96 | -4.95 | -4.34 | -4.67 | -3.72 | -5.13 | -7.90 | -4.84 | -4.74 | -2.44 | -3.28 | -8.45 | -6.56 | -6.76 |
None | -3.70 | -13.91 | -4.56 | -2.70 | -2.22 | -0.97 | -16.13 | -13.76 | -11.03 | 3.15 | -3.53 | -19.00 | -6.77 | -4.56 | -1.36 |
Nuclear Periphery | -1.12 | -24.73 | -10.54 | -10.07 | 6.61 | -0.81 | -24.12 | -19.38 | -16.02 | -0.39 | -0.32 | -32.44 | -19.84 | -18.65 | 5.18 |
Nuclear Periphery Foci | -4.17 | -8.23 | -3.16 | -2.89 | -2.25 | -1.17 | -7.91 | -11.47 | -6.21 | -3.00 | -1.51 | -10.14 | -5.80 | -5.10 | -3.18 |
Nucleolus | -3.91 | 4.36 | -1.69 | 1.29 | -3.59 | -0.62 | 1.89 | -0.64 | 0.23 | -4.10 | -2.74 | 4.79 | -1.15 | 1.44 | -4.76 |
Nucleus | -6.56 | -68.11 | -30.22 | -25.65 | 21.80 | 6.60 | -45.80 | -17.14 | -25.95 | 28.71 | -0.08 | -80.75 | -34.73 | -34.35 | 35.45 |
Peroxisomes | -2.07 | 6.23 | 1.23 | 2.71 | -2.90 | -5.12 | 3.41 | 0.66 | 5.22 | -2.03 | -3.41 | 6.38 | 2.50 | 5.24 | -3.60 |
Vacuole | 11.04 | 62.71* | 22.84 | 11.35 | -24.12 | -7.63 | 32.80* | 16.93 | 33.36* | -21.64 | -1.57 | 66.89* | 28.97 | 30.93 | -32.54 |
Vacuole Periphery | -4.16 | 16.29 | 2.37 | 4.60 | -2.67 | -7.79 | 8.05 | 3.81 | 10.60 | -3.80 | -9.23 | 16.98 | 4.75 | 11.03 | -4.50 |
Temp | Actin Patch (Sac6-tdTomato) | Cortical Patch (Sla1-GFP) | Late Endosome (Snf7-GFP) | Vacuole (Vph1-GFP) |
---|---|---|---|---|
37℃ | ||||
RT |
Gene / Allele | Actin Patch (Sac6-tdTomato) | Cortical Patch (Sla1-GFP) | Late Endosome (Snf7-GFP) | Vacuole (Sac6-tdTomato) |
---|
Gene | Images |
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Gene | Images |
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