Standard name
Human Ortholog
Description v-SNARE component of the vacuolar SNARE complex; involved in vesicle fusion; inhibits ATP-dependent Ca(2+) transport activity of Pmc1p in the vacuolar membrane

Micrographs

ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cell Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cytoplasm 0.29 0.39 0.3 0.55 0.36 0.43 0.55 0.65 0.72 0.71 0.71 0.76 0.81 0.92 0.97 0.32 0.32 0.16 0 0 0 0.07 0.05 0
Endoplasmic Reticulum 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0.05 0.06 0 0 0 0 0 0 0
Endosome 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Golgi 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Mitochondria 0 0 0 0 0 0 0 0 0.07 0.05 0.1 0.06 0 0 0 0 0 0 0 0 0 0 0 0
Nucleus 0.18 0.12 0.34 0.19 0.32 0.21 0.24 0.13 0.07 0.06 0.08 0.06 0 0 0 0.41 0.26 0.31 0 0 0 0 0 0
Nuclear Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Vac/Vac Membrane 0.69 0.66 0.54 0.54 0.65 0.7 0.54 0.46 0.47 0.38 0.37 0.32 0.26 0.1 0 0.55 0.8 0.64 0.91 0.88 0.9 0.81 0.83 0.81
ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 0 1 0 0 0 0 0 0 0 0 0 0 0 2 0 0 0 3
Bud 0 0 0 0 0 0 0 2 1 1 0 1 0 0 0 0 0 0 3 3 1 1 0 2
Bud Neck 0 0 0 0 0 0 0 1 0 0 0 0 0 0 0 0 0 0 1 0 0 0 0 1
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cell Periphery 0 2 0 0 0 0 0 1 0 0 0 1 0 0 0 0 0 0 0 0 0 0 0 0
Cytoplasm 98 151 46 72 79 105 179 216 121 168 120 255 157 221 225 24 16 9 8 14 4 10 8 7
Endoplasmic Reticulum 1 3 1 2 0 3 0 2 0 1 0 0 1 1 1 4 3 2 0 1 1 0 0 2
Endosome 13 6 1 0 3 2 0 1 0 0 0 1 1 1 1 0 0 1 3 5 5 2 5 7
Golgi 0 1 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 2 0 0 0 1
Mitochondria 10 11 2 0 2 3 4 2 12 11 16 20 0 0 1 0 0 2 5 8 0 5 4 4
Nucleus 60 46 52 25 71 52 79 42 11 14 13 19 3 2 2 30 13 18 1 3 0 4 3 1
Nuclear Periphery 0 1 0 1 1 3 1 1 3 2 1 0 0 0 0 0 0 0 0 1 2 0 0 4
Nucleolus 0 0 1 1 0 0 0 0 0 0 1 0 0 0 0 1 0 0 2 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0 1 0 0 0 0 1 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 0 0 0 0 1 0 0 1 0 0 0 2 1 0 1 2 1
Vac/Vac Membrane 232 257 81 71 144 169 177 153 79 90 62 108 51 25 9 41 40 37 336 379 150 131 149 183
Unique Cell Count 338 389 151 132 220 242 328 330 168 235 168 336 194 239 233 74 50 58 368 429 168 162 179 226
Labelled Cell Count 414 478 184 172 300 337 440 422 227 287 213 406 213 250 241 100 72 69 368 429 168 162 179 226


Vacuole

(Huh et al. Nature 2003 425:686)
Screen WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 AF100 AF140 AF180
Mean Cell GFP Intensity (1e-4) 5.2 5.2 5.8 5.5 6.7 5.9 5.5 6.0 4.6 5.1 4.9 5.1 5.2 5.1 4.9 5.4 6.2 7.0 6.1 6.4 6.3
Std Deviation (1e-4) 0.9 1.3 1.1 1.0 1.2 1.4 1.3 1.6 0.9 1.0 1.3 1.1 1.1 1.0 1.7 1.4 1.4 2.4 1.7 1.8 1.7
Intensity Change (Log2) -0.08 0.21 0.03 -0.08 0.04 -0.32 -0.2 -0.25 -0.2 -0.16 -0.18 -0.23 -0.11 0.09 0.28 0.07 0.13 0.12


Localization RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3
Cortical Patches 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cell Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cytoplasm 4.1 1.1 2.6 4.9 7.2 7.4 7.9 7.3 9.5 9.4 12.8 13.9 0.3 0.2 -2.2
Endoplasmic Reticulum 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Endosome 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Golgi 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Mitochondria 0 0 0 0 0 0 1.8 0 2.3 0 0 0 0 0 0
Nucleus -2.9 -0.4 -2.8 -2.4 -5.6 -6.2 -7.3 -5.9 -8.3 -8.3 -9.4 -9.2 0.9 -1.1 -0.5
Nuclear Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Vacuole 0 2.3 3.2 0.1 -1.5 -1.2 -3.0 -3.0 -4.5 -5.2 -9.3 -11.2 0.2 3.3 1.3

Images

Circle plot not available

Protein Concentration and Protein Localization Data

R1 R2 R3
G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase
Concentration 1.6378 2.631 1.8216 1.401 1.5002 2.3614 4.3548 4.959 4.4049 3.8775 3.2875 4.3422 0.942 2.4607 1.6373 1.8921 1.563 2.109
Actin 0.0267 0 0.015 0 0.0002 0.0012 0.0351 0.0001 0.0048 0 0 0 0.0052 0 0.0026 0.0002 0.0088 0.0002
Bud 0.0008 0.0004 0.0005 0.0008 0.0004 0.0005 0.0007 0.0004 0.0009 0.0006 0.0005 0.0002 0.0003 0.0005 0.0007 0.0004 0.001 0.0003
Bud Neck 0.0017 0.0015 0.0018 0.0012 0.001 0.0029 0.0009 0.0007 0.0012 0.0003 0.0025 0.002 0.0008 0.0015 0.0021 0.0009 0.0012 0.0019
Bud Periphery 0.0019 0.0008 0.0016 0.002 0.001 0.0016 0.001 0.0009 0.0017 0.0023 0.0014 0.0009 0.0007 0.001 0.0024 0.0009 0.0023 0.0009
Bud Site 0.006 0.0026 0.0029 0.0028 0.0008 0.0013 0.0019 0.0013 0.0057 0.0006 0.0038 0.0005 0.0009 0.0019 0.0041 0.0015 0.0019 0.0007
Cell Periphery 0.0029 0.0022 0.0026 0.0029 0.0019 0.0031 0.0017 0.0025 0.0039 0.0008 0.0034 0.0019 0.001 0.0016 0.0022 0.0007 0.0012 0.0017
Cytoplasm 0.0246 0.0602 0.0288 0.016 0.0082 0.011 0.0284 0.026 0.0181 0.0025 0.0025 0.007 0.0196 0.0293 0.0182 0.0207 0.0048 0.0141
Cytoplasmic Foci 0.0423 0.0259 0.0259 0.0078 0.0156 0.0089 0.0213 0.0249 0.0134 0.0131 0.0027 0.0054 0.0285 0.0147 0.015 0.0196 0.018 0.015
Eisosomes 0.001 0 0.0006 0 0 0 0.0002 0 0 0 0 0 0.0002 0 0.0001 0 0.0001 0
Endoplasmic Reticulum 0.0059 0.0056 0.0081 0.0025 0.0011 0.0029 0.014 0.0036 0.0079 0.0008 0.0009 0.0022 0.0056 0.0055 0.0053 0.0018 0.006 0.004
Endosome 0.2494 0.1698 0.2592 0.221 0.1785 0.1753 0.2721 0.1186 0.1892 0.3458 0.1218 0.1614 0.3028 0.1916 0.269 0.259 0.2839 0.2119
Golgi 0.0197 0.0027 0.0099 0.003 0.0038 0.0108 0.0115 0.0045 0.006 0.0102 0.0013 0.0031 0.0076 0.0031 0.0061 0.0095 0.0107 0.0035
Lipid Particles 0.0556 0.0177 0.0338 0.0059 0.0068 0.0121 0.0165 0.0225 0.026 0.0063 0.0089 0.0119 0.0249 0.0069 0.0202 0.0047 0.0183 0.0135
Mitochondria 0.0294 0.0046 0.0249 0.0032 0.0475 0.0268 0.0061 0.0093 0.038 0.0425 0.0005 0.0056 0.016 0.016 0.0357 0.0231 0.0214 0.0091
None 0.0652 0.0759 0.033 0.0038 0.006 0.0039 0.0338 0.0141 0.0412 0.0015 0.001 0.0089 0.0543 0.0366 0.0281 0.0081 0.003 0.012
Nuclear Periphery 0.0105 0.0073 0.0148 0.0024 0.0023 0.004 0.0598 0.0083 0.0125 0.0014 0.0017 0.0058 0.0155 0.0069 0.0099 0.0037 0.008 0.0111
Nucleolus 0.0121 0.0044 0.0063 0.004 0.0043 0.0027 0.0028 0.0033 0.006 0.0014 0.0059 0.0047 0.0027 0.0038 0.0083 0.0034 0.003 0.0033
Nucleus 0.012 0.0094 0.0086 0.0051 0.0059 0.0052 0.015 0.008 0.0057 0.0015 0.0051 0.0058 0.0055 0.0076 0.0106 0.0072 0.0044 0.0103
Peroxisomes 0.0366 0.0009 0.0037 0.0008 0.0006 0.001 0.0014 0.0014 0.0034 0.0011 0.0006 0.0013 0.0029 0.0006 0.0034 0.0007 0.0086 0.0011
Punctate Nuclear 0.0036 0.0016 0.0012 0.0002 0.0011 0.0003 0.0086 0.0009 0.0016 0.0001 0.0002 0.0003 0.0038 0.0004 0.0015 0.0012 0.0088 0.0021
Vacuole 0.3425 0.5547 0.4348 0.6378 0.624 0.6397 0.4007 0.5768 0.5279 0.4531 0.7587 0.6464 0.3983 0.5623 0.4556 0.555 0.5052 0.5823
Vacuole Periphery 0.0498 0.0516 0.082 0.0769 0.0888 0.0847 0.0665 0.1722 0.0848 0.1139 0.0765 0.1245 0.1028 0.1083 0.099 0.0777 0.0795 0.1011

Sequencing Data

R1 R2
G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Post-START S/G2 Metaphase Anaphase Telophase
Gene Expression 39.821 29.7725 30.7374 44.4919 33.794 28.9447 46.4633 50.352 47.9555 46.8068
Translational Efficiency 1.3797 1.6798 1.7368 1.2005 1.8466 2.5436 1.2858 1.6625 1.4977 1.5111

Hit Data

Dataset Hit
Protein Concentration
Protein Localization
Gene Expression
Translational Efficiency

Micrographs

Cell Count

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
2161 1661 2106 1222 694 1982 2372 1718 2855 3643 4478 2940

Protein Abundance

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
Mean 720.11 693.95 797.36 745.02 710.84 842.96 811.66 848.61 717.86 775.02 804.93 805.55
Standard Deviation 92.40 105.97 90.36 115.57 76.51 103.31 87.66 121.34 88.88 128.20 89.23 129.47
Intensity Change Log 2 -0.053386 0.147014 0.049062 0.245939 0.191351 0.255577 0.103059 0.169209 0.155343

Localization Score (DeepLoc)

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
Actin 0.000055 0.000095 0.000090 0.001261 0.000071 0.000067 0.000095 0.000355 0.000059 0.000080 0.000093 0.000731
Bud Neck 0.016007 0.017970 0.005751 0.008992 0.015963 0.026562 0.004804 0.013054 0.015997 0.022645 0.005249 0.011366
Bud Site 0.004409 0.011626 0.011947 0.011956 0.004264 0.015309 0.009745 0.044290 0.004374 0.013630 0.010781 0.030851
Cell Periphery 0.000062 0.000098 0.000090 0.000713 0.000102 0.000153 0.000109 0.000117 0.000072 0.000128 0.000100 0.000365
Cytoplasm 0.296817* 0.374519* 0.457723* 0.378904* 0.313567* 0.176174* 0.535703* 0.485850* 0.300889* 0.266608* 0.499029* 0.441398*
Cytoplasmic Foci 0.065132 0.065971 0.007212 0.023143 0.039143 0.044265 0.013064 0.022001 0.058815 0.054162 0.010312 0.022476
Eisosomes 0.000042 0.000034 0.000018 0.000100 0.000044 0.000041 0.000024 0.000029 0.000042 0.000038 0.000021 0.000059
Endoplasmic Reticulum 0.000994 0.000950 0.001408 0.001649 0.001447 0.001722 0.001111 0.001601 0.001104 0.001370 0.001250 0.001621
Endosome 0.039582 0.029982 0.001438 0.009204 0.023300 0.039821 0.002192 0.011594 0.035624 0.035335 0.001837 0.010601
Golgi 0.000867 0.001481 0.000222 0.002247 0.000370 0.001770 0.000160 0.007202 0.000747 0.001638 0.000189 0.005142
Lipid Particles 0.002860 0.002139 0.000585 0.002197 0.002006 0.001713 0.000644 0.000894 0.002653 0.001907 0.000617 0.001435
Mitochondria 0.001236 0.003151 0.000690 0.002113 0.000772 0.003142 0.000368 0.001967 0.001123 0.003146 0.000520 0.002028
Mitotic Spindle 0.002197 0.003428 0.002943 0.011934 0.000363 0.004944 0.005180 0.024102 0.001751 0.004253 0.004128 0.019044
None 0.000613 0.003003 0.004161 0.009483 0.000432 0.000578 0.003697 0.002605 0.000569 0.001683 0.003915 0.005464
Nuclear Periphery 0.000414 0.000464 0.002226 0.001374 0.000279 0.000338 0.001387 0.001225 0.000381 0.000396 0.001781 0.001286
Nuclear Periphery Foci 0.000164 0.000260 0.000343 0.001465 0.000075 0.000179 0.000472 0.000784 0.000142 0.000216 0.000412 0.001067
Nucleolus 0.000968 0.001976 0.000468 0.001960 0.000708 0.000835 0.000507 0.000807 0.000905 0.001355 0.000489 0.001286
Nucleus 0.051905 0.075417 0.460838* 0.314000* 0.062214 0.036675 0.318479* 0.128303 0.054411 0.054339 0.385431* 0.205487*
Peroxisomes 0.001640 0.002955 0.000125 0.001600 0.000650 0.002445 0.000264 0.000948 0.001399 0.002678 0.000199 0.001219
Vacuole 0.511041* 0.399645* 0.041356 0.213263* 0.531288* 0.635888* 0.101507 0.249325* 0.515962* 0.528175* 0.073218 0.234336*
Vacuole Periphery 0.002994 0.004836 0.000365 0.002443 0.002945 0.007377 0.000489 0.002949 0.002982 0.006219 0.000431 0.002738

Localization Changes (T-score)

R1 R2 R1 & R2
UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14 UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14 UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14
Actin -1.68 -3.43 -1.46 -1.39 -1.37 0.08 -0.83 -3.56 -5.32 -3.48 -1.26 -2.60 -1.93 -1.84 -1.72
Bud Neck -1.27 9.88 7.07 6.74 -2.59 -4.98 7.15 2.85 8.77 -6.41 -5.30 12.92 6.48 10.86 -6.63
Bud Site -5.92 -3.21 -4.28 -0.16 -1.27 -7.44 -2.37 -8.89 -4.10 -7.13 -10.09 -4.09 -10.10 -3.78 -6.72
Cell Periphery -2.74 -2.54 -2.87 -2.64 -2.74 -4.34 0.61 1.93 6.40 0.26 -6.83 -1.23 -2.91 -2.01 -2.65
Cytoplasm -8.32 -12.73 -6.46 1.41 4.32 11.99 -14.96 -11.22 -31.64 3.94 4.98 -22.42 -15.36 -20.72 5.04
Cytoplasmic Foci -0.27 27.04 18.70 16.71 -9.76 -1.83 11.55 9.00 13.00 -4.78 1.99 28.49 22.17 21.11 -10.35
Eisosomes 2.55 6.56 -7.99 -10.56 -13.40 0.33 2.58 2.19 5.78 -1.99 1.48 6.53 -2.68 -5.59 -12.84
Endoplasmic Reticulum 0.42 -7.41 -5.13 -6.13 1.99 -1.39 0.03 -0.59 0.91 -0.88 -2.36 -6.28 -4.58 -2.46 0.94
Endosome 4.80 26.22 19.16 12.91 -6.50 -8.35 15.43 10.82 20.73 -6.28 0.20 29.30 22.53 24.04 -9.06
Golgi -1.24 2.77 -0.93 -0.05 -2.10 -3.30 2.64 -3.26 -1.48 -3.56 -2.57 3.25 -2.93 -1.35 -4.12
Lipid Particles 2.49 10.43 3.00 0.52 -5.33 1.15 8.28 6.30 3.69 -6.62 3.52 12.22 6.80 2.88 -6.89
Mitochondria -2.22 2.22 -0.77 1.61 -2.11 -5.17 4.99 -3.04 3.78 -5.37 -4.24 3.18 -1.20 3.48 -4.38
Mitotic Spindle -0.83 -0.96 -4.95 -4.34 -4.67 -3.72 -5.13 -7.90 -4.84 -4.74 -2.44 -3.28 -8.45 -6.56 -6.76
None -3.70 -13.91 -4.56 -2.70 -2.22 -0.97 -16.13 -13.76 -11.03 3.15 -3.53 -19.00 -6.77 -4.56 -1.36
Nuclear Periphery -1.12 -24.73 -10.54 -10.07 6.61 -0.81 -24.12 -19.38 -16.02 -0.39 -0.32 -32.44 -19.84 -18.65 5.18
Nuclear Periphery Foci -4.17 -8.23 -3.16 -2.89 -2.25 -1.17 -7.91 -11.47 -6.21 -3.00 -1.51 -10.14 -5.80 -5.10 -3.18
Nucleolus -3.91 4.36 -1.69 1.29 -3.59 -0.62 1.89 -0.64 0.23 -4.10 -2.74 4.79 -1.15 1.44 -4.76
Nucleus -6.56 -68.11 -30.22 -25.65 21.80 6.60 -45.80 -17.14 -25.95 28.71 -0.08 -80.75 -34.73 -34.35 35.45
Peroxisomes -2.07 6.23 1.23 2.71 -2.90 -5.12 3.41 0.66 5.22 -2.03 -3.41 6.38 2.50 5.24 -3.60
Vacuole 11.04 62.71* 22.84 11.35 -24.12 -7.63 32.80* 16.93 33.36* -21.64 -1.57 66.89* 28.97 30.93 -32.54
Vacuole Periphery -4.16 16.29 2.37 4.60 -2.67 -7.79 8.05 3.81 10.60 -3.80 -9.23 16.98 4.75 11.03 -4.50
Standard name
Human Ortholog
Description v-SNARE component of the vacuolar SNARE complex; involved in vesicle fusion; inhibits ATP-dependent Ca(2+) transport activity of Pmc1p in the vacuolar membrane
Localization
Cell Percentages vacuole (84%)
Cell Cycle Regulation No
Subcompartmental Group N/A

Nyv1

Nyv1


Nearest neighbours ( genes)

correlation threshold

0.6

1.0

0.80


Biological Process (Gene Ontology)

Molecular Function (Gene Ontology)

Cellular Component (Gene Ontology)


Endocytosis
Temp Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Vph1-GFP)
37℃
RT
Cell Cycle Omics

CYCLoPs (Nyv1-GFP)
Gene / Allele Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Sac6-tdTomato)
Gene Images
Gene Images
Images are not yet available
Images are not yet available