Standard name
Human Ortholog
Description Protein of unknown function
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Micrographs

ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 – – – – – –
Cell Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cytoplasm 0.87 0.88 0.91 0.92 0.94 0.88 0.72 0.7 0.74 0.65 0.56 0.67 0.86 0.97 1.0 0.92 0.92 0.9 0.68 0.57 0.58 0.48 0.62 0.58
Endoplasmic Reticulum 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0.05 0.06 0 0 0 0 0 0 0
Endosome 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0.07 0 0 0
Golgi 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Mitochondria 0.12 0.15 0 0.12 0.06 0.36 0.52 0.45 0.38 0.56 0.63 0.5 0 0 0 0 0 0 0 0.05 0.07 0.06 0.09 0.07
Nucleus 0 0 0 0 0 0 0 0 0 0 0 0 0.12 0 0 0 0 0 0 0 0 0 0 0
Nuclear Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Vac/Vac Membrane 0.09 0.06 0.1 0.06 0 0 0 0 0 0 0 0 0.12 0.05 0 0.07 0.08 0.11 0.16 0.27 0.16 0.3 0.16 0.25
ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 1 1 2 1 0 2 1
Bud 0 2 0 1 0 3 6 3 4 15 7 5 0 0 0 0 0 0 0 0 2 1 4 1
Bud Neck 0 0 0 0 0 0 0 1 0 2 0 0 0 0 0 0 0 0 0 2 0 0 0 1
Bud Site 0 0 0 0 0 0 1 1 1 1 0 2 0 0 0 0 0 0 – – – – – –
Cell Periphery 0 0 0 1 2 1 1 1 0 1 1 0 0 0 2 0 0 0 0 0 0 0 0 0
Cytoplasm 68 199 170 181 255 279 258 327 195 246 200 246 82 248 273 182 130 126 77 138 113 83 178 197
Endoplasmic Reticulum 0 2 0 0 0 0 1 0 1 2 2 0 3 1 0 9 9 3 0 0 5 2 3 7
Endosome 0 1 5 1 4 1 1 3 7 3 2 3 0 0 0 2 1 1 3 6 13 5 9 9
Golgi 0 0 1 0 0 0 0 0 0 0 0 0 0 0 0 0 0 2 2 0 2 1 1 0
Mitochondria 9 33 5 24 17 113 185 211 100 212 225 185 1 0 0 6 2 0 1 12 13 9 24 22
Nucleus 1 1 0 1 2 1 0 4 1 3 1 2 11 0 4 0 1 1 1 7 3 3 0 1
Nuclear Periphery 0 2 0 0 0 2 0 4 0 1 1 0 0 0 0 0 0 0 0 0 0 1 1 0
Nucleolus 0 0 0 0 0 0 1 0 0 2 2 1 0 0 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 2 3 0 1 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 1 0 5 4 4 7 7 9 0 0 0 0 0 0 2 2 2 4 4 3
Vac/Vac Membrane 7 14 19 12 10 11 1 4 10 5 6 11 11 14 5 13 11 15 18 66 31 51 46 84
Unique Cell Count 78 226 187 197 271 317 359 467 262 379 359 367 95 256 274 197 141 140 114 245 194 172 286 340
Labelled Cell Count 85 254 200 221 291 411 460 565 326 500 455 464 108 263 284 212 154 149 114 245 194 172 286 340


Cytoplasm, Nucleus

(Huh et al. Nature 2003 425:686)
Screen WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 AF100 AF140 AF180
Mean Cell GFP Intensity (1e-4) 4.0 3.6 4.2 4.2 4.4 3.2 3.3 3.7 3.5 3.1 3.2 3.1 5.6 5.3 5.2 5.5 5.7 5.8 5.0 5.0 5.1
Std Deviation (1e-4) 0.5 0.6 0.8 1.5 2.4 1.5 1.7 1.8 2.2 1.2 1.8 1.4 0.8 0.9 1.3 0.9 1.1 1.0 1.0 1.4 1.2
Intensity Change (Log2) – – – 0.0 0.08 -0.39 -0.32 -0.16 -0.26 -0.44 -0.38 -0.42 0.42 0.35 0.32 0.4 0.44 0.48 0.27 0.25 0.27


Localization RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3
Actin – – – – – – – – – – – – – – –
Bud – – – – – – – – – – – – 0 0 0
Bud Neck – – – – – – – – – – – – 0 0 0
Bud Site – – – – – – – – – – – – 0 0 0
Cell Periphery – – – – – – – – – – – – 0 0 0
Cyto – – – – – – – – – – – – – – –
Endoplasmic Reticulum – – – – – – – – – – – – 0 0 0
Endosome – – – – – – – – – – – – 0 0 0
Golgi – – – – – – – – – – – – 0 0 0
Mitochondria – – – – – – – – – – – – 0 0 0
Nuclear Periphery – – – – – – – – – – – – 0 0 0
Nuc – – – – – – – – – – – – – – –
Nucleolus – – – – – – – – – – – – 0 0 0
Peroxisomes – – – – – – – – – – – – 0 0 0
SpindlePole – – – – – – – – – – – – 0 0 0
Vac – – – – – – – – – – – – – – –
Cortical Patches – – – – – – – – – – – – 0 0 0
Cytoplasm – – – – – – – – – – – – 0 0 0
Nucleus – – – – – – – – – – – – 0 0 0
Vacuole – – – – – – – – – – – – -1.3 -0.7 0.2

Images

Circle plot not available

Protein Concentration and Protein Localization Data

R1 R2 R3
G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase
Concentration -1.2584 -0.5706 -0.9344 -1.0771 -1.1503 -0.9139 -0.0146 0.4135 0.3376 0.3782 0.0847 0.1554 -0.4593 -0.8738 -0.856 -0.4015 -1.4636 -0.6911
Actin 0.0007 0.0005 0.0009 0.0009 0.0103 0.0004 0.0408 0.0001 0.0109 0.0443 0.0189 0.005 0.0554 0.0001 0.0207 0.1152 0.0058 0.0004
Bud 0.0001 0 0.0004 0.0001 0.0017 0.0001 0.0005 0.0001 0.001 0.0005 0.0003 0.0001 0.0005 0 0.0002 0.0005 0.0005 0.0001
Bud Neck 0.0001 0.0001 0.0003 0.0002 0.0028 0.0006 0.0072 0.0002 0.0015 0.0029 0.0016 0.0014 0.0014 0.0001 0.0001 0.0024 0.0002 0.0006
Bud Periphery 0.0001 0 0.0002 0.0001 0.0025 0.0001 0.0011 0 0.0016 0.0005 0.0003 0.0001 0.0006 0 0.0002 0.0005 0.0011 0
Bud Site 0.0003 0.0002 0.0028 0.0001 0.0122 0.0001 0.0059 0.0004 0.0052 0.0029 0.0003 0.0002 0.0043 0.0006 0.003 0.0015 0.0002 0.0001
Cell Periphery 0.0001 0 0.0001 0 0.0001 0 0.0006 0.0001 0.0002 0.0001 0.0001 0 0.0002 0 0.0001 0.0002 0 0
Cytoplasm 0.2687 0.3498 0.318 0.2979 0.1676 0.3667 0.2811 0.4249 0.2869 0.4009 0.3343 0.46 0.2187 0.3853 0.2761 0.2525 0.3821 0.369
Cytoplasmic Foci 0.0124 0.0063 0.0212 0.0334 0.0162 0.017 0.0208 0.0059 0.0152 0.0203 0.0262 0.02 0.0283 0.0046 0.0109 0.0385 0.0539 0.0138
Eisosomes 0.0004 0.0003 0.0002 0.0001 0.0002 0.0001 0.0009 0 0.0001 0.0002 0.0003 0.0002 0.0007 0.0001 0.0001 0.0005 0.0001 0
Endoplasmic Reticulum 0.004 0.0038 0.0033 0.0029 0.0059 0.003 0.0053 0.0023 0.003 0.0018 0.0031 0.0025 0.0104 0.0034 0.0056 0.0046 0.0035 0.0035
Endosome 0.0128 0.0044 0.0111 0.0124 0.011 0.0134 0.0179 0.0027 0.0185 0.0102 0.0161 0.0123 0.0268 0.0017 0.0088 0.1037 0.0189 0.0072
Golgi 0.0005 0.0001 0.0004 0.0007 0.0007 0.0002 0.0032 0.0001 0.0015 0.0051 0.0066 0.0004 0.0078 0.0001 0.0012 0.0513 0.0025 0.0005
Lipid Particles 0.0011 0.0003 0.0012 0.0062 0.0014 0.0004 0.0055 0.0001 0.0024 0.0049 0.0021 0.0005 0.0129 0.0002 0.0043 0.0072 0.0203 0.0015
Mitochondria 0.0007 0.0003 0.0009 0.0016 0.001 0.0005 0.0025 0.0003 0.002 0.0011 0.0036 0.0007 0.0049 0.0002 0.0012 0.0056 0.0019 0.0187
None 0.6509 0.6034 0.5855 0.6029 0.734 0.5415 0.5604 0.5156 0.5933 0.471 0.5341 0.4222 0.5915 0.5671 0.6518 0.3839 0.4378 0.5477
Nuclear Periphery 0.0058 0.0036 0.005 0.0041 0.0027 0.0056 0.0055 0.002 0.0068 0.0019 0.0069 0.0041 0.01 0.0021 0.0069 0.0038 0.0121 0.0022
Nucleolus 0.0006 0.0002 0.0008 0.0014 0.0005 0.0005 0.0012 0.0005 0.0007 0.0005 0.0005 0.0007 0.0005 0.0002 0.0001 0.0003 0.0006 0.0002
Nucleus 0.0207 0.0223 0.0324 0.0204 0.0117 0.0348 0.0246 0.0391 0.0237 0.0204 0.0064 0.0539 0.0068 0.0305 0.0043 0.007 0.0134 0.0214
Peroxisomes 0.0006 0.0002 0.0016 0.004 0.0019 0.0003 0.0052 0.0001 0.0111 0.0038 0.0312 0.0003 0.0048 0.0001 0.0004 0.0068 0.0182 0.0014
Punctate Nuclear 0.0087 0.0019 0.0087 0.0043 0.0086 0.0122 0.0046 0.0034 0.0087 0.0045 0.0036 0.012 0.0104 0.0024 0.0012 0.0014 0.0232 0.0072
Vacuole 0.0057 0.0021 0.0045 0.0051 0.0062 0.002 0.0046 0.002 0.0044 0.0017 0.0024 0.0028 0.0024 0.0011 0.0024 0.0096 0.0024 0.0039
Vacuole Periphery 0.0049 0.0002 0.0008 0.0012 0.0008 0.0005 0.0008 0.0002 0.0012 0.0004 0.001 0.0005 0.0009 0.0001 0.0005 0.0032 0.0013 0.0005

Sequencing Data

R1 R2
G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Post-START S/G2 Metaphase Anaphase Telophase
Gene Expression 35.0984 26.8001 32.013 31.9598 28.4416 36.5405 30.0717 25.4262 28.1745 34.8709
Translational Efficiency 0.9933 1.2235 1.013 1.2191 1.2431 1.1834 1.1117 1.4421 1.3219 1.1244

Hit Data

Dataset Hit
Protein Concentration ✘
Protein Localization ✘
Gene Expression ✘
Translational Efficiency ✘

Micrographs

Cell Count

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
2219 1537 2900 1162 1454 613 2185 2256 3673 2150 5085 3418

Protein Abundance

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
Mean 674.86 746.91 866.14 894.17 650.23 692.79 812.48 903.57 665.11 731.48 843.08 900.37
Standard Deviation 77.78 102.21 108.09 116.63 77.96 90.69 119.72 122.01 78.78 102.03 116.31 120.29
Intensity Change Log 2 — 0.146346 0.360012 0.405961 — 0.091468 0.321382 0.474686 — 0.119678 0.341185 0.440094

Localization Score (DeepLoc)

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
Actin 0.000025 0.000195 0.000199 0.001988 0.000332 0.000305 0.000214 0.000368 0.000146 0.000226 0.000206 0.000919
Bud Neck 0.005941 0.010139 0.011079 0.013606 0.007077 0.024296 0.014360 0.010953 0.006391 0.014175 0.012489 0.011855
Bud Site 0.000801 0.005849 0.007369 0.032927 0.002500 0.014967 0.008074 0.023249 0.001473 0.008448 0.007672 0.026539
Cell Periphery 0.000074 0.000128 0.000103 0.000200 0.000123 0.000219 0.000077 0.000111 0.000094 0.000154 0.000092 0.000141
Cytoplasm 0.511030* 0.518498* 0.263565* 0.341051* 0.509734* 0.371041* 0.396218* 0.412401* 0.510517* 0.476455* 0.320565* 0.388144*
Cytoplasmic Foci 0.112024 0.133097 0.002333 0.012233 0.211991* 0.219597* 0.004945 0.006141 0.151597 0.157760 0.003455 0.008212
Eisosomes 0.000060 0.000058 0.000012 0.000045 0.000126 0.000147 0.000013 0.000020 0.000086 0.000084 0.000012 0.000029
Endoplasmic Reticulum 0.000630 0.001409 0.001957 0.005906 0.000737 0.001810 0.002088 0.002702 0.000672 0.001523 0.002013 0.003791
Endosome 0.003751 0.007783 0.000725 0.008786 0.009141 0.019082 0.001325 0.002624 0.005885 0.011005 0.000983 0.004719
Golgi 0.000528 0.001378 0.000041 0.005039 0.001836 0.003451 0.000477 0.001682 0.001046 0.001969 0.000229 0.002824
Lipid Particles 0.004259 0.004082 0.000307 0.002069 0.008056 0.016655 0.000485 0.000721 0.005762 0.007667 0.000384 0.001179
Mitochondria 0.001171 0.002872 0.001357 0.003368 0.004400 0.013026 0.002052 0.002493 0.002450 0.005767 0.001656 0.002790
Mitotic Spindle 0.000179 0.002818 0.004006 0.048437 0.000964 0.004920 0.007451 0.016036 0.000490 0.003417 0.005487 0.027051
None 0.004596 0.005467 0.005213 0.006413 0.005293 0.004084 0.005295 0.005818 0.004872 0.005073 0.005249 0.006020
Nuclear Periphery 0.000682 0.000710 0.002542 0.003098 0.000505 0.000879 0.003272 0.002666 0.000612 0.000758 0.002856 0.002813
Nuclear Periphery Foci 0.000360 0.000974 0.000193 0.002926 0.000761 0.001319 0.000476 0.001090 0.000519 0.001073 0.000315 0.001714
Nucleolus 0.001075 0.001699 0.000735 0.000604 0.001570 0.003257 0.000833 0.001096 0.001271 0.002143 0.000777 0.000929
Nucleus 0.324171* 0.253742* 0.682420* 0.400065* 0.196091* 0.224424* 0.527853* 0.459217* 0.273469* 0.245383* 0.616003* 0.439107*
Peroxisomes 0.001013 0.003260 0.000235 0.000757 0.004985 0.013680 0.000656 0.000331 0.002585 0.006231 0.000416 0.000476
Vacuole 0.027258 0.044350 0.015409 0.107039 0.032968 0.060618 0.023626 0.049049 0.029518 0.048988 0.018940 0.068764
Vacuole Periphery 0.000372 0.001493 0.000197 0.003442 0.000808 0.002221 0.000207 0.001233 0.000545 0.001700 0.000201 0.001984

Localization Changes (T-score)

R1 R2 R1 & R2
UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14 UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14 UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14
Actin -4.17 -3.02 -3.77 -3.06 -2.99 0.09 0.49 -0.35 -1.04 -2.43 -1.06 -0.91 -3.81 -3.66 -3.73
Bud Neck -3.38 -5.90 -2.92 0.13 1.83 -5.59 -5.37 -4.58 3.85 1.68 -6.24 -7.98 -5.77 2.05 2.15
Bud Site -5.16 -7.08 -7.01 -4.32 -3.82 -4.88 -4.48 -8.19 -0.31 -4.67 -6.85 -8.39 -11.21 -4.63 -6.11
Cell Periphery -3.71 -2.78 -7.50 -1.15 -4.57 -3.52 8.30 3.81 4.48 -3.49 -4.64 1.65 -3.84 2.92 -4.46
Cytoplasm -0.65 40.84 28.48 26.00 -3.15 11.28 17.81 19.74 2.53 2.01 5.03 41.34 33.05 22.09 -4.55
Cytoplasmic Foci -3.87 33.36 30.68 28.74 -6.83 -0.80 39.17 38.91 26.09 -2.59 -1.23 49.46 47.99 38.19 -7.93
Eisosomes 0.31 18.97 0.93 0.61 -18.45 -1.93 15.90 13.65 13.47 -11.11 0.45 22.58 14.10 13.62 -20.79
Endoplasmic Reticulum -5.72 -17.26 -19.16 -15.67 -12.56 -2.89 -14.24 -20.05 -5.37 -5.29 -5.93 -22.40 -27.86 -16.64 -12.91
Endosome -6.52 12.03 -0.46 4.56 -5.58 -5.28 10.45 8.93 9.74 -3.59 -7.06 14.56 7.29 11.78 -7.26
Golgi -2.61 3.78 -1.57 -0.13 -2.45 -1.99 3.80 1.98 3.16 -1.13 -2.73 4.92 -0.00 2.09 -2.63
Lipid Particles 0.41 13.01 7.16 7.53 -10.10 -3.74 12.84 12.14 7.14 -5.55 -2.58 18.01 14.90 9.62 -12.35
Mitochondria -4.94 -0.98 -1.42 2.08 -0.87 -3.13 2.02 2.06 4.39 0.15 -3.78 1.53 0.96 4.91 -0.94
Mitotic Spindle -2.59 -5.49 -6.95 -5.95 -5.56 -2.18 -4.70 -7.99 -3.64 -3.52 -3.27 -7.20 -10.65 -7.69 -6.90
None -1.41 -0.86 0.01 1.32 0.76 1.29 0.69 0.29 -1.32 -0.72 -0.31 -0.10 -0.17 0.19 -0.12
Nuclear Periphery -0.59 -18.77 -18.76 -18.68 -6.34 -3.49 -18.74 -20.94 -17.61 0.84 -2.80 -26.39 -27.89 -26.64 -3.83
Nuclear Periphery Foci -4.31 2.43 -9.16 -5.62 -9.63 -1.41 0.57 -2.14 -0.38 -3.23 -3.50 1.05 -6.65 -3.33 -7.81
Nucleolus -3.54 -1.13 2.33 5.81 2.28 -2.63 0.82 -1.03 1.51 -1.64 -3.73 -0.28 -1.11 1.33 -0.81
Nucleus 9.34 -55.37 -10.77 -17.00 27.25 -2.89 -48.15 -38.60 -24.83 7.63 4.60 -69.35 -32.13 -32.41 26.50
Peroxisomes -5.70 6.65 5.68 7.36 -1.22 -4.63 6.22 6.85 7.59 0.63 -5.69 7.58 8.14 10.14 -0.11
Vacuole -7.56 4.39 -24.76 -21.67 -25.68 -6.96 -2.04 -17.57 -6.85 -15.44 -9.89 1.24 -29.70 -21.67 -29.86
Vacuole Periphery -3.70 3.66 -1.98 0.83 -2.50 -4.49 6.15 0.44 4.32 -3.01 -4.89 7.11 -1.72 2.95 -3.88
Standard name
Human Ortholog
Description Protein of unknown function
Localization
Cell Percentages cytoplasm (25%)
Cell Cycle Regulation No
Subcompartmental Group cytoplasm-9

Gis3

Gis3


Nearest neighbours ( genes)

correlation threshold

0.6

1.0

0.80


Biological Process (Gene Ontology)
Molecular Function (Gene Ontology)
Cellular Component (Gene Ontology)

Endocytosis
Temp Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Vph1-GFP)
37℃
RT
Cell Cycle Omics

CYCLoPs (Gis3-GFP)
Gene / Allele Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Sac6-tdTomato)
Gene Images
Gene Images
Images are not yet available
Images are not yet available