Standard name
Human Ortholog
Description Subunit of the Isw1b complex; exhibits nucleosome-stimulated ATPase activity and acts within coding regions to coordinate transcription elongation with termination and processing; contains a PHD finger motif; other complex members are Isw1p and Ioc4p
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Micrographs

ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0.05 0.05 0 0 0 0 0 0 0 – – – – – –
Cell Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cytoplasm 0.05 0 0 0.12 0 0.14 0.12 0.12 0.06 0.06 0.07 0 0 0.08 0.13 0.07 0.15 0.05 0 0 0 0 0 0
Endoplasmic Reticulum 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Endosome 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Golgi 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Mitochondria 0.05 0.07 0.1 0.12 0 0.2 0.09 0.16 0.29 0.3 0.35 0.13 0 0 0 0.05 0 0 0 0 0 0 0 0
Nucleus 0.84 0.87 0.85 0.94 0.96 0.76 0.77 0.75 0.7 0.71 0.74 0.81 0.85 0.81 0.75 0.76 0.73 0.7 0.79 0.84 0.74 0.58 0.62 0.54
Nuclear Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0.44 0.17 0.33 0.18 0.13 0.18 0.19 0.11 0.28 0.31 0.23 0.31 0.38 0.32 0.29 0.13 0.16 0.15 0.15 0.09 0.16 0.29 0.25 0.3
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Vac/Vac Membrane 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0.05 0 0 0 0 0 0 0.06 0.06
ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 1 0 0 0 1 0 0 0 0 0 0 0 0 0 0 0 1 0 1
Bud 0 0 0 0 0 0 0 2 0 2 1 1 0 0 0 1 0 1 1 2 0 0 2 1
Bud Neck 0 0 0 0 0 0 0 2 0 1 0 2 0 0 0 1 0 0 0 0 0 0 1 3
Bud Site 0 0 0 0 0 1 5 4 7 12 9 5 0 0 1 0 0 0 – – – – – –
Cell Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 1 0 0 0 0 0 0
Cytoplasm 6 7 1 2 3 15 22 24 12 14 12 5 2 24 32 10 19 4 1 1 0 0 0 1
Endoplasmic Reticulum 0 0 0 0 0 0 0 1 0 0 0 0 0 0 0 5 3 0 0 0 0 0 3 1
Endosome 0 2 0 0 0 0 0 0 0 0 0 0 0 0 1 6 1 3 0 2 1 3 1 4
Golgi 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 1 0 2 0 0 0 3 0 1
Mitochondria 6 23 8 2 2 21 16 33 56 74 64 24 5 0 1 8 3 3 0 1 1 1 2 3
Nucleus 98 269 66 16 66 81 143 152 137 175 134 152 129 252 190 114 92 62 92 277 75 117 171 148
Nuclear Periphery 0 1 0 0 0 0 4 1 1 4 2 1 2 1 2 0 2 0 0 0 0 0 0 0
Nucleolus 51 51 26 3 9 19 36 23 55 77 42 59 58 99 73 19 20 13 17 28 16 59 68 83
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0 1 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 1 0 0 3 3 2 0 2 0 3 0 0 1 0 1 1 1 2 0 1 3 2
Vac/Vac Membrane 0 5 0 0 0 0 1 1 1 0 0 1 0 1 0 8 1 3 0 12 3 5 15 17
Unique Cell Count 117 308 78 17 69 107 186 203 195 248 181 188 151 310 254 150 126 88 117 330 102 202 279 275
Labelled Cell Count 161 358 102 23 80 141 230 245 269 362 264 253 196 377 302 173 142 93 117 330 102 202 279 275


Nucleus

(Huh et al. Nature 2003 425:686)
Screen WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 AF100 AF140 AF180
Mean Cell GFP Intensity (1e-4) 6.9 7.3 5.5 5.7 6.7 5.2 5.9 5.9 5.1 4.7 4.6 5.2 7.4 7.8 7.3 9.2 9.8 10.1 7.1 6.9 7.5
Std Deviation (1e-4) 1.3 1.9 0.8 1.0 1.1 1.4 1.6 1.6 1.5 1.3 1.3 1.0 1.6 1.9 1.9 2.5 2.3 2.8 1.5 1.5 1.9
Intensity Change (Log2) – – – 0.05 0.29 -0.09 0.1 0.1 -0.11 -0.22 -0.24 -0.08 0.43 0.5 0.41 0.74 0.83 0.88 0.38 0.33 0.44


Localization RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3
Cortical Patches 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cell Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cytoplasm 0 0 3.0 2.8 2.8 0 0 0 0 0 2.1 2.9 0 3.2 0
Endoplasmic Reticulum 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Endosome 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Golgi 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Mitochondria 0 0 1.7 -0.4 1.3 3.3 0 0 0.6 0 0 0 -1.4 0 -1.8
Nucleus 1.0 2.2 -1.5 -1.4 -1.8 -2.5 -2.5 -1.9 -0.7 0.2 -0.7 -1.8 -1.5 -1.9 -2.2
Nuclear Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus -1.3 -2.9 -2.4 -2.4 -4.4 -0.8 0 0 -0.3 0.8 -0.2 -0.8 -3.7 -2.9 -2.8
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Vacuole 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0

Images

Circle plot not available

Protein Concentration and Protein Localization Data

R1 R2 R3
G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase
Concentration 6.759 6.3299 6.3243 6.2432 5.8604 6.1897 5.4493 4.0912 4.1906 4.1847 3.8229 4.0576 4.92 4.1436 3.7797 3.9665 3.5783 3.6227
Actin 0.024 0 0.0101 0.0002 0.0008 0.0054 0.0329 0.0002 0.006 0.0039 0.0019 0.0022 0.011 0 0.0169 0 0.0002 0.0028
Bud 0.0003 0 0.0002 0 0.0001 0.0002 0.0014 0.0008 0.0026 0.0001 0.0003 0.0003 0.0006 0 0.0004 0 0.0001 0.0002
Bud Neck 0.0014 0 0.0003 0.0001 0.0016 0.0005 0.0007 0.0001 0.0003 0 0.0007 0.0008 0.0009 0 0.0005 0 0.0002 0.0006
Bud Periphery 0.0006 0 0.0005 0.0001 0.0002 0.0004 0.0025 0.0007 0.0034 0.0002 0.0005 0.0008 0.0022 0 0.0008 0 0.0001 0.0009
Bud Site 0.0021 0 0.001 0.0001 0.0002 0.0005 0.0058 0.002 0.0106 0.0002 0.0008 0.0009 0.0019 0 0.0013 0 0.0004 0.0003
Cell Periphery 0.0004 0 0.0002 0 0.0001 0 0.0005 0.0003 0.0003 0 0.0002 0.0001 0.0007 0 0.0002 0 0 0.0002
Cytoplasm 0.0027 0 0.0009 0.0001 0.0005 0.0001 0.0057 0.002 0.0013 0.0004 0.0019 0.0015 0.0021 0 0.0012 0 0.0011 0.0001
Cytoplasmic Foci 0.0093 0 0.003 0.0055 0.0233 0.0002 0.0127 0.0003 0.0018 0.0014 0.0068 0.0012 0.0293 0 0.0104 0 0.0246 0.0002
Eisosomes 0.0004 0 0.0001 0 0 0.0001 0.0003 0 0.0001 0 0.0001 0 0.0001 0 0.0001 0 0 0
Endoplasmic Reticulum 0.0019 0 0.0013 0 0.0002 0.0004 0.0018 0.0003 0.0006 0.0008 0.0014 0.0009 0.0011 0 0.0013 0 0.0001 0.0008
Endosome 0.0098 0 0.0038 0.0006 0.0034 0.0012 0.0245 0.0002 0.0095 0.0098 0.0158 0.0039 0.0143 0 0.0133 0 0.0258 0.0016
Golgi 0.0022 0 0.0018 0.0007 0.0009 0.0006 0.0047 0 0.0018 0.0023 0.0092 0.0013 0.0093 0 0.007 0 0.0091 0.001
Lipid Particles 0.0101 0 0.0045 0.0135 0.0111 0.0003 0.0059 0.0001 0.0037 0.0037 0.0549 0.0008 0.012 0 0.0175 0 0.001 0.0012
Mitochondria 0.0071 0 0.0048 0.0005 0.0008 0.0006 0.0086 0.0011 0.0122 0.0096 0.0518 0.0026 0.0094 0.0001 0.0241 0.0001 0.001 0.0037
None 0.003 0 0.0007 0.0002 0.0011 0.0002 0.0056 0.0004 0.0004 0.0005 0.0005 0.0002 0.0023 0 0.0017 0 0.0001 0.0002
Nuclear Periphery 0.004 0.0001 0.0041 0.0014 0.0047 0.0018 0.0142 0.0006 0.0035 0.0153 0.0048 0.0037 0.0086 0.0003 0.0043 0.0001 0.0006 0.0022
Nucleolus 0.0539 0.0302 0.0278 0.0415 0.2961 0.1043 0.0394 0.0286 0.0135 0.0111 0.1653 0.1258 0.0523 0.0349 0.0252 0.0153 0.1169 0.0948
Nucleus 0.8487 0.9694 0.9268 0.8882 0.6422 0.8822 0.7858 0.9598 0.9077 0.9354 0.6719 0.8503 0.8206 0.9645 0.8512 0.9845 0.8145 0.8872
Peroxisomes 0.0058 0 0.0015 0.0456 0.0055 0.0002 0.0134 0.0001 0.0029 0.0012 0.0038 0.0002 0.0062 0 0.0132 0 0.0028 0.0001
Punctate Nuclear 0.0075 0.0002 0.0039 0.0014 0.007 0.0006 0.0251 0.0004 0.0115 0.0021 0.0036 0.0008 0.0088 0.0001 0.0044 0 0.0009 0.0006
Vacuole 0.0031 0 0.0018 0.0002 0.0003 0.0001 0.0051 0.0014 0.0037 0.0004 0.002 0.0009 0.005 0 0.0024 0 0.0002 0.0007
Vacuole Periphery 0.0015 0 0.001 0.0002 0.0002 0.0001 0.0033 0.0005 0.0025 0.0015 0.002 0.0007 0.0015 0 0.0026 0 0.0002 0.0007

Sequencing Data

R1 R2
G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Post-START S/G2 Metaphase Anaphase Telophase
Gene Expression 15.3816 19.4121 23.4075 21.8056 19.0631 23.0596 24.4322 22.4927 23.4821 23.5468
Translational Efficiency 1.3128 0.8491 0.6981 0.7645 0.84 0.7162 0.7864 0.7212 0.6773 0.8073

Hit Data

Dataset Hit
Protein Concentration ✘
Protein Localization ✘
Gene Expression ✘
Translational Efficiency ✘

Micrographs

Cell Count

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
1186 1702 2260 1492 2110 1750 2049 230 3296 3452 4309 1722

Protein Abundance

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
Mean 722.46 907.17 1110.07 1204.43 849.36 945.55 1083.07 1243.18 803.70 926.63 1097.23 1209.61
Standard Deviation 93.49 166.06 173.83 189.15 128.00 147.80 154.17 192.72 131.69 158.23 165.32 190.09
Intensity Change Log 2 — 0.328455 0.619661 0.737361 — 0.154778 0.350678 0.549587 — 0.237209 0.480562 0.638938

Localization Score (DeepLoc)

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
Actin 0.000383 0.000624 0.000730 0.000990 0.000316 0.000598 0.000705 0.001662 0.000340 0.000611 0.000718 0.001080
Bud Neck 0.005490 0.010111 0.022828 0.028092 0.006928 0.010410 0.020137 0.026573 0.006410 0.010262 0.021549 0.027889
Bud Site 0.000782 0.001651 0.002032 0.003366 0.000688 0.001790 0.001219 0.004087 0.000722 0.001722 0.001646 0.003462
Cell Periphery 0.000100 0.000097 0.000082 0.000083 0.000123 0.000090 0.000046 0.000085 0.000115 0.000093 0.000065 0.000083
Cytoplasm 0.004930 0.001206 0.000591 0.001520 0.000652 0.000260 0.000206 0.001057 0.002191 0.000726 0.000408 0.001458
Cytoplasmic Foci 0.001091 0.000498 0.000067 0.001155 0.000136 0.000814 0.000034 0.004644 0.000480 0.000658 0.000051 0.001621
Eisosomes 0.000027 0.000044 0.000061 0.000058 0.000040 0.000057 0.000066 0.000057 0.000036 0.000050 0.000064 0.000058
Endoplasmic Reticulum 0.001986 0.002938 0.001294 0.001240 0.002142 0.002775 0.001448 0.001124 0.002086 0.002855 0.001367 0.001224
Endosome 0.000266 0.000576 0.000113 0.001915 0.000227 0.000947 0.000085 0.004294 0.000241 0.000764 0.000100 0.002233
Golgi 0.000445 0.000358 0.000151 0.001490 0.000155 0.000640 0.000121 0.002790 0.000259 0.000501 0.000137 0.001663
Lipid Particles 0.001029 0.000222 0.000050 0.000110 0.000077 0.000269 0.000025 0.000350 0.000419 0.000246 0.000038 0.000142
Mitochondria 0.004894 0.001265 0.001452 0.002727 0.000755 0.003016 0.001273 0.002249 0.002244 0.002152 0.001367 0.002663
Mitotic Spindle 0.001715 0.001284 0.003499 0.013622 0.001400 0.007481 0.002260 0.018945 0.001513 0.004425 0.002910 0.014333
None 0.009908 0.002556 0.003191 0.001672 0.002622 0.000927 0.001231 0.003263 0.005244 0.001730 0.002259 0.001885
Nuclear Periphery 0.000223 0.000172 0.000184 0.001023 0.000173 0.000363 0.000131 0.000436 0.000191 0.000269 0.000159 0.000945
Nuclear Periphery Foci 0.000426 0.001024 0.000102 0.000793 0.000549 0.000841 0.000123 0.000318 0.000505 0.000931 0.000112 0.000730
Nucleolus 0.078934 0.098846 0.107183 0.096529 0.096330 0.118582 0.112964 0.072822 0.090070 0.108851 0.109932 0.093362
Nucleus 0.885684* 0.874779* 0.854771* 0.838810* 0.885857* 0.846860* 0.856307* 0.849253* 0.885795* 0.860626* 0.855501* 0.840205*
Peroxisomes 0.001101 0.000907 0.001183 0.001784 0.000403 0.001597 0.000958 0.002750 0.000654 0.001257 0.001076 0.001913
Vacuole 0.000358 0.000531 0.000228 0.002044 0.000204 0.000730 0.000369 0.002698 0.000259 0.000632 0.000295 0.002131
Vacuole Periphery 0.000229 0.000314 0.000208 0.000978 0.000223 0.000953 0.000292 0.000543 0.000225 0.000638 0.000248 0.000920

Localization Changes (T-score)

R1 R2 R1 & R2
UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14 UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14 UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14
Actin -3.54 -8.34 -13.24 -8.97 -7.94 -4.99 -14.08 -5.94 -4.97 -4.51 -6.48 -16.61 -16.62 -10.87 -9.32
Bud Neck -5.43 -20.13 -18.37 -15.11 -5.30 -5.16 -17.54 -10.48 -9.00 -4.58 -7.43 -27.45 -21.23 -17.57 -7.14
Bud Site -2.30 -3.25 -5.84 -3.79 -3.04 -3.09 -4.77 -4.57 -3.60 -4.05 -3.89 -4.67 -7.22 -4.83 -4.95
Cell Periphery 0.17 1.05 0.74 0.55 -0.53 0.46 1.18 0.09 -0.81 -4.45 0.48 1.25 0.58 0.15 -2.21
Cytoplasm 3.34 4.20 2.66 -0.96 -2.56 1.99 1.89 -1.80 -2.36 -2.34 3.34 4.47 0.54 -2.32 -3.22
Cytoplasmic Foci 1.51 3.16 0.75 -0.58 -1.92 -2.27 1.61 -1.15 -0.75 -1.21 -0.66 3.43 -1.09 -0.68 -2.30
Eisosomes -5.11 -11.58 -10.90 -7.41 0.48 -3.73 -7.66 -6.00 -2.56 -1.02 -5.22 -12.17 -10.66 -5.06 0.68
Endoplasmic Reticulum -3.81 3.85 2.66 6.53 -2.05 -2.56 4.52 0.42 2.40 -2.26 -4.44 6.38 4.29 7.54 -2.07
Endosome -1.80 2.81 -1.93 -1.26 -2.24 -2.31 1.98 -1.37 -0.86 -1.47 -2.91 2.92 -2.34 -1.23 -2.65
Golgi 0.44 2.04 -0.99 -1.14 -1.53 -2.24 0.51 -1.24 -0.76 -1.26 -1.91 1.96 -1.62 -1.10 -1.84
Lipid Particles 1.54 1.89 1.77 1.11 -1.74 -1.84 2.40 -1.88 -0.19 -2.34 0.86 2.03 1.48 1.43 -3.21
Mitochondria 2.39 2.18 1.14 -8.98 -10.69 -2.93 -5.17 -8.78 -0.82 -6.83 0.13 1.34 -1.79 -2.61 -13.16
Mitotic Spindle 0.74 -2.28 -5.91 -6.26 -5.02 -3.49 -1.11 -3.44 -2.49 -3.29 -3.00 -2.51 -6.97 -5.35 -6.19
None 6.13 5.50 6.77 2.20 1.90 4.31 3.26 -2.26 -5.47 -4.79 6.90 5.55 5.91 -2.17 -0.20
Nuclear Periphery 1.07 0.66 -3.08 -3.31 -3.23 -2.23 0.37 -4.55 -3.27 -4.64 -1.62 0.47 -3.73 -3.32 -3.80
Nuclear Periphery Foci -1.27 3.23 -0.36 1.05 -2.84 -0.40 1.89 0.77 0.85 -1.65 -1.02 2.77 -0.02 1.02 -2.98
Nucleolus -4.53 -8.23 -5.14 -1.54 1.83 -5.76 -4.56 0.58 2.94 2.57 -6.54 -8.47 -3.33 0.97 2.51
Nucleus 1.64 6.97 8.69 8.19 3.47 7.99 7.70 5.35 2.47 2.79 6.86 10.97 11.54 7.06 4.82
Peroxisomes 0.60 -0.85 -3.20 -5.06 -4.65 -3.48 -8.51 -6.95 -3.13 -5.45 -2.76 -4.77 -9.57 -4.14 -7.48
Vacuole -0.81 0.21 -3.36 -3.12 -3.63 -3.44 -1.65 -2.88 -2.44 -2.65 -3.00 -1.17 -4.40 -3.63 -4.15
Vacuole Periphery -1.37 0.23 -2.67 -2.31 -2.84 -2.38 -0.66 -2.56 1.30 -1.59 -2.62 -0.48 -2.98 -0.50 -2.78
Standard name
Human Ortholog
Description Subunit of the Isw1b complex; exhibits nucleosome-stimulated ATPase activity and acts within coding regions to coordinate transcription elongation with termination and processing; contains a PHD finger motif; other complex members are Isw1p and Ioc4p
Localization
Cell Percentages nucleus (98%)
Cell Cycle Regulation No
Subcompartmental Group nucleus-4

Ioc2

Ioc2


Nearest neighbours ( genes)

correlation threshold

0.6

1.0

0.80


Biological Process (Gene Ontology)
Molecular Function (Gene Ontology)
Cellular Component (Gene Ontology)

Endocytosis
Temp Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Vph1-GFP)
37℃
RT
Cell Cycle Omics

CYCLoPs (Ioc2-GFP)
Gene / Allele Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Sac6-tdTomato)
Gene Images
Gene Images
Images are not yet available
Images are not yet available