Standard name
Human Ortholog
Description 3-keto sterol reductase; catalyzes the last of three steps required to remove two C-4 methyl groups from an intermediate in ergosterol biosynthesis; mutants are sterol auxotrophs; mutation is functionally complemented by human HSD17B7
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Micrographs

ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0.08 0.13 0.22 0.17 0.24 0.11 0 0.07 0 0 0 0 0.12 0.05 0 0.08 0.1 0.13 0.19 0.2 0.18 0.14 0.15 0.12
Bud 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 – – – – – –
Cell Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cytoplasm 0 0 0 0.05 0 0.07 0 0 0 0 0 0 0 0.05 0 0.05 0.05 0 0 0 0 0 0 0
Endoplasmic Reticulum 0 0.05 0.06 0 0.14 0.06 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0.07 0 0 0
Endosome 0.11 0 0.05 0.06 0.09 0.06 0.07 0 0 0 0 0 0.09 0.07 0.08 0 0 0 0.17 0.25 0.18 0.15 0.14 0.14
Golgi 0.07 0.09 0.47 0.34 0.63 0.35 0.1 0.08 0 0 0 0 0.26 0.28 0.39 0.74 0.75 0.66 0.27 0.29 0.28 0.29 0.23 0.26
Mitochondria 0.73 0.77 0.43 0.48 0.23 0.47 0.73 0.7 0.8 0.76 0.84 0.78 0.35 0.11 0.1 0.1 0.09 0.08 0 0 0 0 0 0.05
Nucleus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nuclear Periphery 0.05 0.09 0.06 0.05 0 0.08 0.06 0.07 0.07 0.07 0.08 0.07 0 0.09 0.05 0 0 0 0 0 0 0 0 0
Nucleolus 0.24 0.2 0.13 0.16 0.08 0.16 0.21 0.26 0.27 0.24 0.26 0.23 0.33 0.5 0.45 0.16 0.21 0.21 0 0 0 0 0 0
Peroxisomes 0.08 0 0.09 0.08 0 0 0 0 0 0 0 0 0.07 0 0 0 0 0 0.23 0.09 0.2 0.27 0.29 0.31
SpindlePole 0 0 0.05 0.07 0 0 0 0 0 0 0 0 0.1 0.12 0.1 0 0 0 0 0 0 0 0 0
Vac/Vac Membrane 0 0 0 0 0 0.07 0 0 0.06 0.07 0 0.06 0 0 0 0.07 0 0 0 0.05 0 0 0 0
ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 19 12 26 32 56 28 17 22 9 5 2 3 15 7 3 34 14 40 50 25 27 16 29 21
Bud 0 1 2 4 4 3 5 4 4 4 0 5 0 1 0 2 1 4 0 0 0 0 2 1
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 1 0 0 0 1 0
Bud Site 0 0 0 1 0 0 0 1 1 3 0 1 0 0 0 0 0 0 – – – – – –
Cell Periphery 0 1 1 0 2 0 2 0 0 0 1 0 0 1 0 1 2 0 0 0 0 0 0 0
Cytoplasm 7 0 2 9 6 17 13 7 10 4 5 7 2 7 1 20 7 13 0 0 0 0 0 0
Endoplasmic Reticulum 1 5 7 0 32 15 8 0 2 1 0 0 1 4 2 10 6 14 9 1 10 2 5 1
Endosome 26 0 6 12 20 15 30 10 1 4 0 11 11 9 13 8 3 5 46 31 28 18 28 24
Golgi 17 8 56 64 145 90 44 26 5 10 0 3 33 36 60 303 106 206 72 36 43 34 43 46
Mitochondria 177 72 51 91 53 122 323 229 259 308 300 312 44 14 16 39 12 26 6 5 3 4 7 9
Nucleus 0 0 0 0 0 0 1 2 1 2 4 2 0 1 0 3 0 1 0 0 0 0 0 0
Nuclear Periphery 13 8 7 10 5 20 27 22 21 27 27 29 4 12 8 11 1 7 2 4 3 2 4 2
Nucleolus 57 19 15 30 19 42 93 86 87 96 94 93 42 64 70 65 29 65 1 1 0 1 0 0
Peroxisomes 19 2 11 16 2 0 6 3 5 8 0 4 9 4 6 2 0 4 62 11 30 31 56 55
SpindlePole 6 0 6 13 7 5 4 4 0 4 0 3 13 15 16 13 3 8 8 0 0 1 4 6
Vac/Vac Membrane 3 2 5 3 10 19 8 5 18 29 11 24 2 2 4 30 2 12 4 6 3 3 6 5
Unique Cell Count 241 94 119 191 229 259 442 327 323 405 358 398 127 129 155 410 141 313 267 127 154 120 195 179
Labelled Cell Count 345 130 195 285 361 376 581 421 423 505 444 497 176 177 199 541 186 405 267 127 154 120 195 179


Lipid Particles

(Huh et al. Nature 2003 425:686)
Screen WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 AF100 AF140 AF180
Mean Cell GFP Intensity (1e-4) 13.7 17.9 15.0 14.7 15.8 14.8 14.5 15.6 13.9 14.7 13.5 14.1 17.8 18.8 18.8 21.8 24.7 23.6 14.7 15.4 15.8
Std Deviation (1e-4) 2.8 4.4 2.6 2.3 2.5 2.6 2.8 3.2 3.3 3.4 3.1 3.3 3.7 4.0 3.1 5.4 6.1 6.1 2.8 3.5 3.4
Intensity Change (Log2) – – – -0.03 0.08 -0.02 -0.05 0.05 -0.12 -0.04 -0.16 -0.09 0.25 0.33 0.32 0.54 0.72 0.65 -0.03 0.04 0.08

WT3RAP60RAP140RAP220RAP300RAP380RAP460RAP540RAP620RAP7000102030WT3HU80HU120HU1600102030WT3rpd3Δ_1rpd3Δ_2rpd3Δ_30102030WT1AF100AF140AF1800102030
rapamycinhydroxy-urearpd3 knockoutalpha-factorMultiple Condition Time-Course Intensity PlotsGFP Intensity (1e-4)GFP Intensity (1e-4)GFP Intensity (1e-4)GFP Intensity (1e-4)

Localization RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3
Cortical Patches -1.1 0.5 -2.8 -6.6 -4.6 -6.6 -8.4 -8.6 -8.8 -2.1 -3.8 -5.3 -4.1 -2.7 -2.3
Bud 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cell Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cytoplasm 0 0 2.0 0 0 0 0 0 0 0 0 0 0 0 0
Endoplasmic Reticulum 0 2.3 0 0 0 0 0 0 0 0 -1.1 0 0 -0.6 -0.6
Endosome 0.5 1.2 0.3 0.7 0 0 0 0 0 1.1 0.6 1.1 0 0 0
Golgi -2.4 2.9 -2.3 -9.4 -9.4 -12.3 -12.9 -13.8 -13.9 -3.4 -3.1 -1.4 5.5 4.7 3.6
Mitochondria 0.8 -3.8 0.8 6.2 5.3 7.6 6.9 8.8 7.4 -1.3 -5.7 -6.2 -8.5 -6.4 -8.4
Nucleus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nuclear Periphery -0.2 0 0.6 0.1 0.3 0.2 0.3 0.6 0.5 -1.0 1.0 -0.3 0 0 0
Nucleolus 0.8 -1.3 0.9 2.1 3.1 3.2 2.6 3.1 2.5 3.8 6.2 5.8 0.9 1.7 2.0
Peroxisomes -0.3 0 0 0 0 0 0 0 0 -0.6 -2.0 -1.8 0 -3.7 0
SpindlePole 0.6 0 0 0 0 0 0 0 0 1.5 1.9 1.6 0 0 0
Vacuole 0 0.1 1.2 0 0 0.6 1.2 0 0.8 0 0 0 1.2 0 0

Images

Circle plot not available

Protein Concentration and Protein Localization Data

R1 R2 R3
G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase
Concentration 7.119 10.3679 9.3564 8.0656 8.4028 9.6533 7.8502 9.6932 8.7427 8.8421 7.7909 9.0553 7.3571 10.7697 9.1182 7.7607 7.3324 10.3102
Actin 0.0277 0.0027 0.0083 0.0039 0.0011 0.0132 0.0049 0.0054 0.0111 0.0006 0.0008 0.0008 0.0562 0.0016 0.0177 0.0282 0.0096 0.0243
Bud 0.0004 0.0002 0.0002 0.0001 0.0001 0.0001 0.0001 0 0.0001 0 0 0 0.0005 0 0.0003 0.0002 0.0001 0
Bud Neck 0.0007 0.0004 0.0013 0.0004 0.0001 0.0005 0.0007 0.0002 0.0003 0.0002 0.0001 0.0001 0.0032 0.0001 0.0015 0.0015 0.0003 0.0002
Bud Periphery 0.0006 0.0003 0.0003 0.0002 0.0001 0.0002 0.0001 0 0.0002 0.0001 0 0 0.0006 0 0.0005 0.0003 0.0002 0
Bud Site 0.0039 0.0013 0.0047 0.0006 0.0003 0.0003 0.0016 0.0001 0.0012 0.0001 0.0001 0.0001 0.0098 0.0001 0.0066 0.0008 0.0002 0.0001
Cell Periphery 0.0004 0.0005 0.0005 0.0002 0 0.0002 0.0002 0.0001 0.0001 0.0004 0.0001 0.0001 0.0005 0.0003 0.0003 0.0002 0.0002 0.0001
Cytoplasm 0.013 0.0006 0.0009 0.0003 0.0001 0.0008 0.0009 0.0001 0.0001 0.0001 0 0 0.0174 0.0001 0.0004 0.0008 0.0066 0
Cytoplasmic Foci 0.0674 0.0667 0.0587 0.047 0.0562 0.0308 0.0537 0.0433 0.0536 0.0589 0.0505 0.026 0.0629 0.0474 0.0576 0.0374 0.0258 0.027
Eisosomes 0.0006 0 0.0001 0.0001 0 0.0001 0.0002 0 0.0001 0 0 0 0.0003 0 0.0002 0.0003 0.0002 0
Endoplasmic Reticulum 0.0089 0.0007 0.0012 0.0009 0.0001 0.0024 0.001 0.0001 0.0003 0.0003 0 0.0003 0.0015 0.0004 0.0014 0.0038 0.0093 0.0002
Endosome 0.06 0.0363 0.031 0.0373 0.0083 0.0206 0.0144 0.0152 0.0221 0.0388 0.0165 0.0075 0.0137 0.0126 0.022 0.0396 0.0429 0.0043
Golgi 0.0505 0.0551 0.0647 0.041 0.0283 0.0409 0.0273 0.03 0.0615 0.0866 0.0542 0.0301 0.0271 0.0458 0.0491 0.0951 0.0585 0.0279
Lipid Particles 0.6384 0.7168 0.7257 0.7703 0.8188 0.8117 0.801 0.8558 0.7661 0.7244 0.7859 0.8922 0.6922 0.8571 0.7603 0.7408 0.7131 0.8628
Mitochondria 0.0363 0.0126 0.013 0.0471 0.0113 0.0428 0.0128 0.0064 0.0193 0.0089 0.0109 0.0064 0.0315 0.0023 0.0193 0.0143 0.0802 0.0049
None 0.0078 0.0002 0.0005 0.0001 0.0001 0.0004 0.0033 0 0.0002 0 0 0 0.0167 0 0.0009 0.0007 0.0108 0
Nuclear Periphery 0.0108 0.0156 0.0027 0.0009 0.0004 0.0048 0.0051 0.001 0.0035 0.0004 0.0001 0.0024 0.0032 0.0004 0.0022 0.0052 0.0199 0.0006
Nucleolus 0.0146 0.0266 0.0308 0.0003 0.001 0.003 0.0291 0.0133 0.0007 0.0007 0.0004 0.006 0.0104 0.0018 0.0012 0.0005 0.0007 0.0165
Nucleus 0.0112 0.0026 0.0022 0.0002 0.0002 0.001 0.002 0.0005 0.0001 0.0001 0 0.0002 0.0038 0.0001 0.0005 0.0005 0.0049 0.0006
Peroxisomes 0.025 0.0222 0.0388 0.0442 0.0359 0.0171 0.0255 0.0232 0.0539 0.0726 0.0781 0.022 0.023 0.018 0.0442 0.0229 0.0096 0.0174
Punctate Nuclear 0.0138 0.0242 0.0036 0.0011 0.0363 0.0034 0.0119 0.0008 0.0011 0.0007 0.0005 0.0014 0.0209 0.0073 0.0058 0.0031 0.0017 0.0109
Vacuole 0.0032 0.0071 0.0064 0.0023 0.0003 0.002 0.0013 0.0013 0.0013 0.0024 0.0005 0.0008 0.0013 0.001 0.0026 0.0013 0.0017 0.0004
Vacuole Periphery 0.0048 0.007 0.0044 0.0015 0.0009 0.0037 0.003 0.0031 0.0029 0.0036 0.0012 0.0035 0.0034 0.0034 0.0053 0.0026 0.0037 0.0017

Sequencing Data

R1 R2
G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Post-START S/G2 Metaphase Anaphase Telophase
Gene Expression 96.178 102.0389 114.9781 122.822 92.4965 98.2374 135.1737 137.3333 142.364 134.7439
Translational Efficiency 1.3385 1.347 1.35 1.2721 1.1229 1.4371 1.2992 1.3859 1.163 1.0922

Hit Data

Dataset Hit
Protein Concentration ✘
Protein Localization ✘
Gene Expression ✔
Translational Efficiency ✘

Micrographs

Cell Count

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
933 307 798 1990 1714 2225 1892 1286 2647 2532 2690 3276

Protein Abundance

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
Mean 1424.93 1657.65 1841.88 1698.89 1469.52 1508.29 1661.24 1667.64 1453.80 1526.40 1714.83 1686.62
Standard Deviation 230.89 291.30 313.40 360.98 270.28 253.25 319.94 363.20 257.97 262.72 328.55 362.17
Intensity Change Log 2 — 0.218248 0.370288 0.253701 — 0.037569 0.176916 0.182463 — 0.129345 0.275351 0.217973

Localization Score (DeepLoc)

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
Actin 0.016884 0.012082 0.079933 0.084518 0.019068 0.012080 0.055417 0.083951 0.018299 0.012080 0.062690 0.084295
Bud Neck 0.001881 0.002922 0.001141 0.004320 0.000900 0.000950 0.002736 0.004572 0.001246 0.001190 0.002263 0.004419
Bud Site 0.000528 0.000707 0.000591 0.002677 0.000198 0.000370 0.001208 0.002512 0.000314 0.000411 0.001025 0.002612
Cell Periphery 0.000075 0.000043 0.000046 0.000185 0.000040 0.000045 0.000101 0.000240 0.000052 0.000044 0.000085 0.000207
Cytoplasm 0.000266 0.000284 0.000262 0.000778 0.000142 0.000388 0.000425 0.000661 0.000186 0.000375 0.000376 0.000732
Cytoplasmic Foci 0.035796 0.057280 0.017745 0.058791 0.017840 0.037368 0.045410 0.050648 0.024169 0.039782 0.037203 0.055594
Eisosomes 0.001112 0.001130 0.001680 0.000950 0.000804 0.001083 0.000822 0.001087 0.000912 0.001089 0.001077 0.001004
Endoplasmic Reticulum 0.001928 0.000690 0.000996 0.004117 0.003234 0.000596 0.002226 0.006969 0.002773 0.000608 0.001861 0.005237
Endosome 0.007832 0.008194 0.007516 0.044497 0.005725 0.005470 0.022741 0.045775 0.006468 0.005800 0.018224 0.044999
Golgi 0.073752 0.093058 0.146925 0.212845 0.052951 0.083652 0.139115 0.242412 0.060283 0.084792 0.141432 0.224451
Lipid Particles 0.843254 0.793905 0.728623 0.518055 0.891591 0.837086 0.703027 0.480117 0.874554 0.831851 0.710620 0.503162
Mitochondria 0.005652 0.006165 0.008274 0.033280 0.002113 0.007022 0.009707 0.042275 0.003361 0.006918 0.009282 0.036811
Mitotic Spindle 0.000674 0.002320 0.000224 0.005259 0.000281 0.001381 0.003346 0.003245 0.000419 0.001495 0.002420 0.004468
None 0.000137 0.000216 0.000167 0.000304 0.000290 0.000194 0.000149 0.000409 0.000236 0.000197 0.000154 0.000345
Nuclear Periphery 0.000666 0.002328 0.000400 0.002065 0.000550 0.000717 0.000980 0.002616 0.000591 0.000912 0.000808 0.002281
Nuclear Periphery Foci 0.003124 0.003640 0.000226 0.002916 0.001085 0.002991 0.001581 0.002577 0.001804 0.003070 0.001179 0.002783
Nucleolus 0.000142 0.002358 0.000032 0.000219 0.000037 0.000516 0.000133 0.000226 0.000074 0.000740 0.000103 0.000222
Nucleus 0.000053 0.000274 0.000017 0.000091 0.000025 0.000093 0.000054 0.000104 0.000035 0.000115 0.000043 0.000096
Peroxisomes 0.003977 0.006475 0.002534 0.004706 0.001921 0.005477 0.006200 0.005356 0.002646 0.005598 0.005112 0.004961
Vacuole 0.001728 0.004463 0.001461 0.011889 0.000769 0.001752 0.002928 0.012893 0.001107 0.002081 0.002492 0.012283
Vacuole Periphery 0.000539 0.001464 0.001207 0.007538 0.000436 0.000768 0.001696 0.011356 0.000472 0.000852 0.001551 0.009037

Localization Changes (T-score)

R1 R2 R1 & R2
UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14 UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14 UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14
Actin 1.53 -9.20 -14.68 -14.98 -0.58 2.88 -9.34 -11.84 -13.74 -4.71 3.28 -13.09 -18.56 -21.15 -4.88
Bud Neck -1.44 1.59 -4.62 -1.79 -7.77 -0.25 -5.22 -6.55 -6.69 -3.04 0.16 -3.57 -9.04 -9.86 -5.87
Bud Site -0.57 -0.25 -4.95 -3.65 -4.39 -2.77 -7.04 -7.67 -7.00 -3.95 -1.29 -5.48 -8.08 -7.63 -5.23
Cell Periphery 1.56 1.46 -5.11 -11.40 -11.42 -0.66 -6.13 -10.93 -10.44 -7.05 0.79 -3.37 -12.77 -14.65 -10.55
Cytoplasm -0.31 0.10 -3.62 -3.59 -3.85 -3.24 -11.62 -2.95 -1.45 -1.36 -2.76 -7.60 -5.05 -2.92 -3.37
Cytoplasmic Foci -4.08 7.98 -8.34 -0.26 -17.75 -12.26 -14.62 -14.35 -5.38 -1.95 -9.85 -8.48 -18.20 -8.09 -9.63
Eisosomes -0.07 -2.51 0.92 0.92 4.65 -1.11 -0.12 -1.77 -0.01 -1.98 -0.81 -1.31 -0.80 0.43 0.82
Endoplasmic Reticulum 2.70 2.00 -3.36 -7.04 -6.32 3.82 1.31 -2.93 -5.89 -4.19 4.55 1.71 -3.54 -8.95 -5.94
Endosome -0.21 0.27 -18.38 -15.55 -18.67 0.29 -11.68 -17.38 -17.82 -9.03 0.91 -10.83 -25.77 -26.46 -15.85
Golgi -2.48 -9.68 -22.33 -14.11 -7.89 -9.03 -18.30 -27.01 -22.19 -13.14 -7.89 -20.18 -36.76 -29.67 -15.47
Lipid Particles 3.49 9.77 33.34 18.51 17.17 9.32 24.44 41.17 35.41 19.78 8.03 25.28 55.28 46.72 26.16
Mitochondria -0.23 -1.22 -9.55 -8.52 -8.01 -4.81 -5.70 -10.63 -9.09 -8.20 -3.55 -5.13 -15.06 -12.84 -11.49
Mitotic Spindle -1.91 2.05 -4.42 -2.24 -4.88 -3.03 -3.93 -3.41 -1.98 0.12 -3.19 -3.62 -5.66 -3.85 -2.36
None -2.67 -1.65 -6.42 -2.49 -5.16 0.54 0.79 -0.62 -5.77 -7.16 0.34 0.71 -0.91 -6.35 -8.87
Nuclear Periphery -1.98 1.56 -4.67 0.30 -6.12 -1.21 -2.10 -3.57 -3.31 -2.74 -2.11 -1.44 -5.90 -4.57 -4.89
Nuclear Periphery Foci -0.38 3.70 0.16 0.52 -5.42 -3.01 -1.10 -3.13 0.56 -1.64 -2.04 1.43 -2.19 0.42 -3.56
Nucleolus -1.71 3.06 -1.20 1.64 -3.03 -3.01 -2.55 -2.69 1.68 -1.20 -3.11 -1.00 -3.11 2.37 -2.31
Nucleus -1.81 2.33 -1.81 1.50 -4.57 -3.19 -2.44 -2.36 -0.26 -1.45 -3.30 -0.82 -3.57 0.69 -3.03
Peroxisomes -1.20 1.62 -0.93 0.86 -2.87 -4.58 -5.19 -3.70 0.14 0.84 -4.22 -3.86 -4.03 0.89 0.21
Vacuole -1.77 0.37 -9.55 -3.92 -8.76 -2.66 -5.98 -9.67 -8.75 -7.85 -2.66 -4.24 -13.87 -11.95 -11.76
Vacuole Periphery -3.00 -3.46 -10.06 -8.01 -8.84 -2.10 -7.63 -8.86 -8.54 -7.79 -2.67 -8.33 -13.33 -12.47 -11.46
Protein Complexes26S Proteasome complex37S mitochondrial small ribosomal subunit54S mitochondrial large ribosomal subunit60S cytosolic large ribosomal subunitAar2-U5 snRNPAnaphase-Promoting Complex variant 1Anaphase-Promoting Complex variant 2Anaphase-Promoting Complex variant 3Anaphase-Promoting Complex variant 4Arp2/3 complexATG1 kinase complexBLOC-1 complexCcr4-Not complexCentral kinetochore CTF19 complexCleavage and polyadenylation specificity factorcomplexCMG helicase complexCMG-Pol epsilon complexCOMPASS complexCOP9 signalosome COPI vesicle coat complexCoQ6 biosynthesis complexCore mediator complexCORVET complexCTF18-RFC complexCURI complex variant 1CURI complex variant 2CURI complex variant 3Cytoplasmic exosome complex, DIS3 variantDASH complexDNA-directed RNA Polymerase I complexDNA-directed RNA polymerase II complexDNA-directed RNA polymerase III complexDNA replication factor C complexEGO complexELG1-RFC complex Elongator holoenzyme complexEndoplasmic Reticulum Membrane ComplexEukaryotic translation initiation factor 2BcomplexEukaryotic translation initiation factor 3core complexExocystExomer complexFAR complexGeneral transcription factor complex TFIIDGeneral transcription factor complex TFIIHGET4-GET5 transmembrane domain recognition complexGID ubiquitin ligase complexGin4 complexGolgi transport complexGSE complexHMC complexHOPS complexINO80 chromatin remodeling complexLSM1-7 complexLsm1-7-Pat1 complexLSM2-7 complexLSM2-8 complexMCM complexMICOS complexMitochondrial electron transport complex IIIMitochondrial inner membrane pre-sequence translocasecomplexMitochondrial outer membrane translocase holocomplexMitochondrial proton-transporting ATP synthase complexMitochondrial pyruvate dehydrogenase complexNineTeen complexNOP8 60s ribosome pre-assembly complexNuA3 histone acetyltransferase complexNuA4 histone acetyltransferase complexNuclear condensin complexNuclear/nucleolar exosome complex, DIS3-RRP6 variantNuclear pore complexNucleolar ribonuclease MRP complexNucleolar ribonuclease P complexOligosaccharyl transferase complex variant 1Oligosaccharyl transferase complex variant 2PAF1 complexPAS complexPhosphatidylinositol 3-kinase complex IPolarisomePrefoldin complexPRP19-associated complexRad17 RFC-like complexRetromer complexRPD3L histone deacetylase complexRPD3S histone deacetylase complexRQC complexRSC complex variant RSC1RSC complex variant RSC2SAGA complexSCF-Met30 ubiquitin ligase complexSCF-Ufo1 ubiquitin ligase complexSEA complexSeptin complexSET3C histone deacetylase complexSignal recognition particleSLIK (SAGA-like) complexSmall ribosomal subunit processome, variant1Small ribosomal subunit processome, variant2Small ribosomal subunit processome, variant3SMC5-SMC6 SUMO ligase complexSm complexSpliceosomal commitment complexSPOTS complexSWI/SNF chromatin remodelling complexSwr1 chromatin remodelling complexTHO complexTranscription factor TFIIIC complexTranslocon complexTRAPPIII protein complexTRAPPII protein complexTRAPPI protein complexTREX complexU1 snRNPU2 snRNPU4 snRNPU4/U6 snRNPU4/U6.U5 tri-snRNP complexU5 snRNPU6 snRNP complexUTP-A complexUTP-B complexUTP-C complex variant 1UTP-C complex variant 2UTP-C complex variant 3Vacuolar proton translocating ATPase complex,Golgi variantVacuolar proton translocating ATPase complex,vacuole variantCellular Components••••••••••••••••actinbud neckcell peripherycytoplasmendosomeERGolgimitochondrionmixednuclear peripherynucleolusnucleusperoxisomespindle polevacuolar membranevacuole
Standard name
Human Ortholog
Description 3-keto sterol reductase; catalyzes the last of three steps required to remove two C-4 methyl groups from an intermediate in ergosterol biosynthesis; mutants are sterol auxotrophs; mutation is functionally complemented by human HSD17B7
Localization
Cell Percentages —
Cell Cycle Regulation No
Subcompartmental Group N/A

Erg27

Erg27


Nearest neighbours ( genes)

correlation threshold

0.6

1.0

0.80


Biological Process (Gene Ontology)
Molecular Function (Gene Ontology)
Cellular Component (Gene Ontology)

Endocytosis
Temp Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Vph1-GFP)
37℃
RT
Cell Cycle Omics

CYCLoPs (Erg27-GFP)
Gene / Allele Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Sac6-tdTomato)
Gene Images
Gene Images
Images are not yet available
Images are not yet available