Standard name
Human Ortholog
Description RNA exonuclease; required for maturation of the RNA component of RNase MRP; functions redundantly with Rnh70p and Rex2p in processing of U5 snRNA and RNase P RNA; member of RNase D family of exonucleases
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Micrographs

ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud 0 0 0 0 0 0 0 0 0 0 0 0.05 0 0 0 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 – – – – – –
Cell Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cytoplasm 0.07 0.13 0.21 0.22 0.27 0.29 0.16 0.23 0.11 0.15 0.11 0.14 0.23 0.33 0.46 0.12 0.16 0.16 0 0.13 0.06 0.14 0.13 0.13
Endoplasmic Reticulum 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0.07 0.05 0 0 0 0 0 0 0
Endosome 0 0 0.12 0.06 0.09 0 0 0 0 0 0 0 0 0 0 0.07 0.06 0.06 0 0 0 0 0 0
Golgi 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Mitochondria 0.77 0.25 0.17 0.35 0.26 0.6 0.77 0.66 0.8 0.85 0.87 0.83 0 0 0 0.12 0.07 0.09 0.05 0 0.08 0 0 0.06
Nucleus 0.33 0.56 0.46 0.41 0.22 0.26 0.28 0.26 0.27 0.25 0.26 0.26 0.69 0.58 0.4 0.64 0.59 0.56 0.49 0.52 0.41 0.5 0.36 0.31
Nuclear Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0.05 0 0 0.06 0.07 0.05 0.09 0.1
Vac/Vac Membrane 0.09 0.17 0.14 0.1 0.27 0.12 0.07 0.08 0.08 0 0.07 0.08 0 0.08 0.13 0.17 0.2 0.24 0.32 0.17 0.25 0.19 0.25 0.25
ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 2 0 0 1
Bud 0 0 0 2 1 4 6 5 4 10 3 18 0 0 1 0 0 1 1 2 0 5 9 12
Bud Neck 0 0 0 0 1 0 0 0 0 0 1 1 2 1 0 0 0 0 0 0 0 2 4 6
Bud Site 0 0 0 0 0 4 8 7 4 10 8 15 0 0 0 0 1 0 – – – – – –
Cell Periphery 0 0 0 0 0 1 0 0 0 0 0 1 0 0 0 0 0 1 0 0 0 0 0 0
Cytoplasm 3 17 29 32 47 73 58 68 28 56 27 55 77 140 147 34 38 28 3 20 12 34 55 55
Endoplasmic Reticulum 0 1 0 0 1 0 0 0 0 1 0 0 1 3 3 19 11 7 0 0 1 0 1 0
Endosome 0 1 17 9 16 3 2 5 0 0 0 1 9 13 8 19 14 11 0 2 8 3 5 14
Golgi 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 2 1 0 0 1 1 0 0 1
Mitochondria 33 33 23 52 46 151 272 199 209 312 210 331 9 2 3 34 18 15 4 5 15 7 16 27
Nucleus 14 74 63 60 39 66 98 79 71 93 62 104 234 242 129 183 144 98 39 81 81 127 147 132
Nuclear Periphery 0 1 0 0 0 1 0 1 0 4 0 5 1 0 0 0 1 0 0 0 1 1 1 3
Nucleolus 0 1 1 1 2 2 1 0 2 0 1 1 0 1 0 4 3 1 0 1 3 4 11 9
Peroxisomes 0 0 0 0 0 0 0 1 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 2 0 3 5 2 5 3 2 1 2 9 5 3 7 11 1 3 9 14 13 38 41
Vac/Vac Membrane 4 22 19 15 47 30 26 23 20 12 18 30 12 34 43 48 48 42 25 26 49 48 102 105
Unique Cell Count 43 132 136 147 176 250 352 301 261 369 242 397 339 420 320 287 243 176 80 156 197 255 411 430
Labelled Cell Count 54 150 154 171 203 340 473 393 341 500 331 564 354 441 337 350 290 205 80 156 197 255 411 430


Cytoplasm, Nucleus

(Huh et al. Nature 2003 425:686)
Screen WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 AF100 AF140 AF180
Mean Cell GFP Intensity (1e-4) 3.3 4.3 4.4 4.4 4.1 3.6 3.2 3.4 3.1 3.0 2.8 2.9 5.4 5.5 5.3 6.1 6.2 6.2 5.2 5.7 5.9
Std Deviation (1e-4) 0.3 0.8 1.5 2.6 1.9 2.1 1.5 1.6 0.8 1.1 1.1 1.0 1.0 1.1 1.3 1.4 1.8 1.7 1.1 1.4 1.5
Intensity Change (Log2) – – – -0.01 -0.09 -0.27 -0.43 -0.36 -0.5 -0.57 -0.63 -0.62 0.29 0.32 0.27 0.48 0.51 0.51 0.25 0.38 0.43


Localization RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3
Actin – – – – – – – – – – – – – – –
Bud – – – – – – – – – – – – 0 0 0
Bud Neck – – – – – – – – – – – – 0 0 0
Bud Site – – – – – – – – – – – – 0 0 0
Cell Periphery – – – – – – – – – – – – 0 0 0
Cyto – – – – – – – – – – – – – – –
Endoplasmic Reticulum – – – – – – – – – – – – 0 0 0
Endosome – – – – – – – – – – – – 0 0 0
Golgi – – – – – – – – – – – – 0 0 0
Mitochondria – – – – – – – – – – – – 0 0 0
Nuclear Periphery – – – – – – – – – – – – 0 0 0
Nuc – – – – – – – – – – – – – – –
Nucleolus – – – – – – – – – – – – 0 0 0
Peroxisomes – – – – – – – – – – – – 0 0 0
SpindlePole – – – – – – – – – – – – 0 0 0
Vac – – – – – – – – – – – – – – –
Cortical Patches – – – – – – – – – – – – 0 0 0
Cytoplasm – – – – – – – – – – – – 0 0 0
Nucleus – – – – – – – – – – – – 0 0 0
Vacuole – – – – – – – – – – – – 0.7 1.4 2.2

Images

Circle plot not available

Protein Concentration and Protein Localization Data

R1 R2 R3
G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase
Concentration 1.4113 0.564 0.6092 1.1349 0.9794 0.6734 -0.2325 -0.1535 -0.2131 -0.4881 -0.1135 -0.4647 -1.2504 -0.6245 -1.0513 -0.8759 -1.263 -0.9889
Actin 0.0292 0 0.0016 0.0042 0.0024 0.0011 0.0252 0.0007 0.0064 0.0671 0.0212 0.0063 0.016 0.0002 0.0021 0.0145 0.0102 0.0072
Bud 0.0003 0 0 0.0009 0.0001 0.0001 0.0012 0.0003 0.0003 0.0003 0.0024 0.0001 0.0003 0 0.0002 0.0006 0.0001 0.0002
Bud Neck 0.012 0.0003 0.0009 0.0009 0.0003 0.0017 0.0033 0.0005 0.0019 0.0005 0.0047 0.0028 0.0088 0.0003 0.0025 0.0034 0.0007 0.0025
Bud Periphery 0.0004 0 0.0001 0.0007 0.0001 0.0001 0.0022 0.0002 0.0003 0.0004 0.0033 0.0002 0.0006 0 0.0005 0.0017 0.0002 0.0005
Bud Site 0.0023 0.0002 0.0003 0.0004 0.0002 0.0002 0.0087 0.0022 0.0036 0.0012 0.0097 0.0002 0.0029 0.0003 0.0005 0.0017 0.0003 0.0005
Cell Periphery 0.0002 0 0.0002 0.0001 0.0001 0 0.0007 0 0.0001 0.0001 0.0004 0 0.0002 0 0.0001 0.0002 0 0
Cytoplasm 0.0339 0.0096 0.0276 0.0465 0.0056 0.0106 0.0244 0.0209 0.0248 0.0128 0.0238 0.0068 0.0151 0.0087 0.0143 0.0283 0.0042 0.0089
Cytoplasmic Foci 0.013 0.0001 0.0007 0.0021 0.0011 0.0011 0.037 0.0024 0.0124 0.0147 0.0209 0.0005 0.0055 0.0004 0.005 0.0007 0.0119 0.0021
Eisosomes 0.0004 0 0.0001 0.0001 0.0002 0 0.0005 0 0.0001 0.0002 0.0001 0 0.0004 0 0.0001 0.0002 0 0.0001
Endoplasmic Reticulum 0.0092 0.0004 0.0052 0.0022 0.0012 0.0049 0.0067 0.0012 0.0012 0.0002 0.0022 0.0008 0.0027 0.0008 0.0011 0.0016 0.001 0.0016
Endosome 0.0193 0.0003 0.0019 0.0024 0.0096 0.0028 0.0315 0.0015 0.0089 0.0095 0.047 0.0018 0.0118 0.0007 0.0057 0.0063 0.0086 0.006
Golgi 0.0041 0 0.0002 0.0002 0.0033 0.0003 0.0103 0.0002 0.0087 0.018 0.0272 0.0004 0.0029 0 0.0011 0.0047 0.0051 0.0025
Lipid Particles 0.0055 0 0.0004 0.0002 0.0343 0.0003 0.0201 0.0002 0.0061 0.0221 0.0196 0.0003 0.0067 0.0001 0.0042 0.0018 0.018 0.0042
Mitochondria 0.0021 0.0003 0.0006 0.0027 0.0303 0.0005 0.0297 0.0006 0.0098 0.018 0.0066 0.0004 0.0101 0.0003 0.0055 0.0017 0.0009 0.0014
None 0.1012 0.0209 0.0585 0.1215 0.0095 0.0481 0.0177 0.0242 0.0321 0.0008 0.0033 0.0019 0.0243 0.0114 0.033 0.006 0.001 0.0029
Nuclear Periphery 0.0268 0.0065 0.0342 0.0447 0.0482 0.0186 0.0352 0.021 0.0232 0.02 0.0116 0.0092 0.0333 0.0256 0.0248 0.0184 0.0521 0.0128
Nucleolus 0.013 0.0085 0.0069 0.0048 0.0099 0.0198 0.0128 0.011 0.0083 0.0047 0.0177 0.0182 0.0095 0.0071 0.0089 0.0072 0.0081 0.014
Nucleus 0.6806 0.9322 0.8264 0.7253 0.8255 0.8615 0.6888 0.8901 0.8156 0.7755 0.7381 0.9279 0.826 0.9092 0.8565 0.8664 0.8099 0.9015
Peroxisomes 0.0044 0 0 0.0003 0.0024 0.0001 0.0251 0.0003 0.0065 0.0292 0.0078 0.0001 0.0016 0.0001 0.0014 0.0003 0.0211 0.0014
Punctate Nuclear 0.0393 0.0197 0.0332 0.0376 0.0122 0.0271 0.012 0.0203 0.0268 0.0038 0.0259 0.0213 0.018 0.0343 0.0307 0.0323 0.0458 0.0283
Vacuole 0.0016 0.0007 0.0007 0.0013 0.0012 0.0009 0.0054 0.0018 0.0019 0.0005 0.0046 0.0005 0.0018 0.0005 0.0011 0.0014 0.0003 0.0009
Vacuole Periphery 0.0011 0.0001 0.0003 0.0011 0.0025 0.0003 0.0016 0.0004 0.0012 0.0005 0.0017 0.0002 0.0012 0.0002 0.0008 0.0007 0.0004 0.0005

Sequencing Data

R1 R2
G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Post-START S/G2 Metaphase Anaphase Telophase
Gene Expression 18.163 17.8603 20.4821 15.094 13.5408 18.153 17.0325 14.9584 15.3271 18.6368
Translational Efficiency 0.8247 0.7855 0.6309 0.6985 0.8395 0.7958 0.7148 1.0648 0.829 0.7658

Hit Data

Dataset Hit
Protein Concentration ✘
Protein Localization ✘
Gene Expression ✘
Translational Efficiency ✘

Micrographs

Cell Count

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
221 443 1844 1204 1623 1610 399 1077 1844 2053 2243 2281

Protein Abundance

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
Mean 689.01 787.68 854.36 874.16 669.19 748.41 914.90 888.18 671.57 756.88 865.13 880.78
Standard Deviation 79.29 96.93 111.09 121.76 87.34 89.84 118.61 133.73 86.66 92.83 114.82 127.75
Intensity Change Log 2 — 0.193085 0.310319 0.343372 — 0.161413 0.451198 0.408436 — 0.177567 0.381450 0.375796

Localization Score (DeepLoc)

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
Actin 0.000166 0.001133 0.000424 0.000889 0.000122 0.000279 0.000387 0.001089 0.000127 0.000464 0.000417 0.000983
Bud Neck 0.002196 0.009312 0.008202 0.015247 0.001768 0.007821 0.001936 0.016929 0.001820 0.008143 0.007087 0.016041
Bud Site 0.000714 0.002734 0.001245 0.009366 0.000713 0.003975 0.000435 0.009547 0.000714 0.003707 0.001101 0.009451
Cell Periphery 0.000161 0.000166 0.000062 0.000114 0.000118 0.000297 0.000111 0.000154 0.000123 0.000269 0.000071 0.000133
Cytoplasm 0.103996* 0.063105 0.010898 0.047577 0.241191* 0.086986 0.028589 0.040787 0.224749* 0.081833 0.014045 0.044371
Cytoplasmic Foci 0.036763 0.020630 0.000338 0.007609 0.019089 0.022781 0.003027 0.002483 0.021207 0.022317 0.000817 0.005188
Eisosomes 0.000069 0.000079 0.000018 0.000029 0.000026 0.000053 0.000040 0.000030 0.000031 0.000059 0.000022 0.000029
Endoplasmic Reticulum 0.002330 0.009011 0.005036 0.006159 0.001089 0.002976 0.005040 0.009556 0.001238 0.004278 0.005037 0.007763
Endosome 0.001532 0.002610 0.000373 0.004527 0.001106 0.003047 0.000436 0.002830 0.001157 0.002953 0.000384 0.003726
Golgi 0.000219 0.001286 0.000050 0.004039 0.000207 0.001106 0.000037 0.002988 0.000209 0.001145 0.000048 0.003542
Lipid Particles 0.007710 0.007534 0.000243 0.001602 0.001848 0.005190 0.000761 0.000694 0.002550 0.005696 0.000335 0.001173
Mitochondria 0.005539 0.005834 0.001425 0.002735 0.000753 0.010183 0.000425 0.004322 0.001326 0.009245 0.001247 0.003485
Mitotic Spindle 0.001087 0.009421 0.003266 0.068976 0.000271 0.010199 0.000074 0.032519 0.000369 0.010031 0.002698 0.051762
None 0.009223 0.006182 0.002966 0.008631 0.023908 0.007355 0.006818 0.008971 0.022148 0.007102 0.003651 0.008792
Nuclear Periphery 0.002016 0.003844 0.001130 0.003118 0.000770 0.003409 0.000687 0.004643 0.000919 0.003503 0.001051 0.003838
Nuclear Periphery Foci 0.002025 0.006396 0.000261 0.001604 0.000801 0.000876 0.000244 0.002353 0.000948 0.002067 0.000258 0.001958
Nucleolus 0.006334 0.009608 0.003226 0.006439 0.004042 0.008131 0.001592 0.006731 0.004316 0.008449 0.002935 0.006577
Nucleus 0.809915* 0.818837* 0.957652* 0.792811* 0.697957* 0.811655* 0.943831* 0.835663* 0.711375* 0.813205* 0.955194* 0.813044*
Peroxisomes 0.002913 0.012005 0.000164 0.001355 0.000521 0.003471 0.000156 0.000665 0.000808 0.005312 0.000163 0.001029
Vacuole 0.004731 0.008882 0.002909 0.015536 0.003412 0.009009 0.005306 0.014131 0.003570 0.008981 0.003335 0.014872
Vacuole Periphery 0.000361 0.001391 0.000111 0.001638 0.000287 0.001201 0.000067 0.002915 0.000296 0.001242 0.000103 0.002241

Localization Changes (T-score)

R1 R2 R1 & R2
UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14 UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14 UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14
Actin -5.93 -8.48 -14.19 0.05 -8.99 -3.44 -7.86 -10.90 -9.68 -6.08 -6.53 -11.74 -16.49 -10.73 -10.51
Bud Neck -4.03 -9.64 -13.60 -5.81 -7.31 -7.64 -4.19 -12.69 -9.61 -11.44 -8.66 -11.99 -18.70 -12.44 -11.43
Bud Site -3.98 -3.30 -8.15 -4.78 -7.33 -3.59 -2.16 -8.54 -5.38 -8.03 -4.36 -4.61 -11.83 -6.50 -10.73
Cell Periphery -0.48 5.15 3.15 4.66 -5.93 -1.25 0.43 -4.10 0.83 -3.54 -1.32 7.04 -1.55 1.19 -8.41
Cytoplasm 3.69 9.66 6.83 4.32 -9.01 21.05 30.35 31.02 12.28 -2.51 21.71 36.60 31.64 12.98 -10.64
Cytoplasmic Foci 3.07 7.85 7.33 6.25 -3.27 -1.87 11.68 11.88 12.21 0.08 -0.63 15.53 13.64 13.54 -3.88
Eisosomes -1.06 6.75 2.75 4.18 -12.13 -4.36 -5.78 -9.17 -0.24 -0.57 -5.37 1.92 -9.18 1.23 -12.13
Endoplasmic Reticulum -7.22 -8.84 -13.72 -0.09 -6.97 -10.49 -9.43 -21.41 -17.04 -3.09 -12.26 -20.10 -29.33 -15.99 -9.57
Endosome -2.08 4.16 -2.17 0.19 -6.14 -4.07 2.87 -4.15 0.74 -5.97 -4.68 5.05 -5.24 0.60 -8.77
Golgi -1.94 3.53 -2.10 -0.20 -2.45 -2.76 3.63 -2.15 -0.64 -2.42 -3.32 4.26 -3.04 -0.69 -3.44
Lipid Particles 0.28 4.81 4.36 5.81 -3.06 -6.32 4.83 4.12 8.17 -1.24 -5.91 8.66 5.66 9.86 -4.75
Mitochondria -0.12 1.07 0.65 2.69 -4.09 -7.45 1.93 -4.57 4.51 -4.80 -7.19 -0.10 -3.78 5.50 -4.93
Mitotic Spindle -3.08 -2.12 -12.25 -9.61 -11.74 -5.14 -1.16 -10.34 -8.15 -9.46 -6.02 -3.44 -16.15 -12.91 -15.04
None 2.02 4.72 0.80 -1.69 -6.14 8.66 8.34 7.68 -1.72 -2.54 8.97 11.76 8.07 -1.90 -8.27
Nuclear Periphery -3.41 1.87 -6.08 -2.41 -7.92 -8.44 -3.64 -5.76 -2.41 -5.34 -9.68 -6.84 -9.72 -3.74 -8.61
Nuclear Periphery Foci -2.30 2.28 -1.79 1.63 -5.58 -0.46 3.14 -6.43 -6.31 -6.78 -2.62 3.63 -7.68 -4.51 -8.55
Nucleolus -2.38 2.83 -6.08 -4.02 -7.71 -5.18 3.32 -8.36 -6.44 -9.26 -5.98 1.61 -10.95 -7.96 -11.55
Nucleus -0.30 -9.85 2.39 3.13 20.88 -11.29 -24.57 -5.52 3.96 15.26 -11.00 -33.68 -5.29 5.52 27.50
Peroxisomes -2.97 4.71 3.70 3.83 -3.26 -7.86 4.26 -2.22 6.08 -4.09 -6.47 6.50 -0.01 6.43 -5.24
Vacuole -2.44 1.12 -9.42 -6.82 -10.27 -5.83 -4.30 -8.67 -5.64 -3.41 -6.54 -3.35 -13.48 -9.25 -11.99
Vacuole Periphery -1.64 2.62 -1.43 0.97 -2.59 -3.24 1.94 -1.57 -0.19 -1.89 -3.62 2.03 -2.07 0.52 -2.70
Standard name
Human Ortholog
Description RNA exonuclease; required for maturation of the RNA component of RNase MRP; functions redundantly with Rnh70p and Rex2p in processing of U5 snRNA and RNase P RNA; member of RNase D family of exonucleases
Localization
Cell Percentages nucleus (62%)
Cell Cycle Regulation No
Subcompartmental Group nucleus-3

Rex3

Rex3


Nearest neighbours ( genes)

correlation threshold

0.6

1.0

0.80


Biological Process (Gene Ontology)
Molecular Function (Gene Ontology)
Cellular Component (Gene Ontology)

Endocytosis
Temp Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Vph1-GFP)
37℃
RT
Cell Cycle Omics

CYCLoPs (Rex3-GFP)
Gene / Allele Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Sac6-tdTomato)
Gene Images
Gene Images
Images are not yet available
Images are not yet available