Standard name
Human Ortholog
Description Member of the NineTeen Complex (NTC); this complex contains Prp19p and stabilizes U6 snRNA in catalytic forms of the spliceosome containing U2, U5, and U6 snRNAs; homolog of Drosophila crooked neck protein; interacts with U1 snRNP proteins
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Micrographs

ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 – – – – – –
Cell Periphery 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cytoplasm 0 0 0 0.06 0.06 0.06 0 – 0 0.06 0 0 0.13 0.17 0.17 0.09 0.1 0.07 0 0 0 0 0 0
Endoplasmic Reticulum 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Endosome 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Golgi 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Mitochondria 0.22 0 0 0 0.05 0.13 0.11 – 0.36 0.27 0.5 0.5 0 0 0 0 0 0 0 0 0 0 0 0
Nucleus 0.93 0.95 0.94 0.91 0.89 0.84 0.9 – 0.87 0.8 0.79 0.79 0.86 0.81 0.79 0.83 0.8 0.87 0.92 0.93 0.88 0.88 0.83 0.77
Nuclear Periphery 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 0 0 0 0 – 0 0 0.08 0.08 0 0 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Vac/Vac Membrane 0 0 0 0 0.05 0 0 – 0 0 0 0 0 0 0 0.07 0.06 0 0 0 0.06 0.05 0.08 0.13
ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud 0 0 0 0 0 1 0 – 1 1 2 2 0 0 0 0 0 0 1 1 1 1 1 1
Bud Neck 0 0 1 0 0 0 0 – 1 1 0 0 0 0 1 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 1 1 4 4 – 6 4 8 7 0 1 0 0 0 0 – – – – – –
Cell Periphery 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cytoplasm 3 6 6 11 11 16 13 – 6 15 9 9 42 50 80 21 25 11 2 0 2 2 5 1
Endoplasmic Reticulum 0 1 0 0 0 0 0 – 0 0 0 0 1 0 0 7 4 3 0 0 0 0 0 1
Endosome 0 0 1 4 3 2 1 – 0 0 0 0 1 2 5 1 3 2 0 1 3 0 4 4
Golgi 0 0 1 0 0 0 0 – 0 0 0 0 0 1 1 1 0 1 0 1 0 0 1 3
Mitochondria 35 10 0 6 9 36 34 – 75 64 101 101 0 2 3 6 5 3 0 1 1 2 6 7
Nucleus 147 435 170 162 170 234 269 – 180 194 160 160 285 242 375 194 192 138 143 440 165 187 352 268
Nuclear Periphery 0 2 0 3 1 7 0 – 1 1 3 3 5 3 1 3 4 2 0 0 0 1 0 2
Nucleolus 3 4 0 0 1 4 3 – 7 7 17 17 0 1 2 2 1 4 0 5 0 4 11 8
Peroxisomes 0 0 0 0 0 0 0 – 0 0 0 0 0 1 1 0 0 0 0 0 0 0 0 0
SpindlePole 0 3 1 2 0 3 2 – 2 3 1 1 2 6 7 0 1 3 1 1 0 1 2 2
Vac/Vac Membrane 1 20 3 3 10 10 5 – 1 3 1 2 3 4 10 17 15 6 5 14 10 10 34 44
Unique Cell Count 158 459 181 179 191 278 299 206 241 203 203 330 299 472 233 239 158 155 473 187 214 426 351
Labelled Cell Count 189 481 183 192 206 317 331 280 293 302 302 339 313 486 252 250 173 155 473 187 214 426 351


Nucleus

(Huh et al. Nature 2003 425:686)
Screen WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 AF100 AF140 AF180
Mean Cell GFP Intensity (1e-4) 4.9 6.7 6.8 6.1 6.7 5.3 5.4 – 4.5 4.9 4.0 4.0 6.9 6.7 6.5 8.5 8.9 8.2 6.3 6.3 6.5
Std Deviation (1e-4) 0.8 1.0 1.8 1.5 2.1 1.8 1.5 – 1.1 1.6 0.9 0.9 1.4 1.4 1.5 2.0 2.0 2.0 1.4 1.2 1.3
Intensity Change (Log2) – – – -0.16 -0.03 -0.35 -0.34 – -0.6 -0.47 -0.75 -0.75 0.01 -0.02 -0.07 0.32 0.38 0.28 -0.11 -0.11 -0.06


Localization RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3
Cortical Patches 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0
Bud 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0
Cell Periphery 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0
Cytoplasm 1.3 1.1 1.2 0.6 – -0.2 1.4 0.6 0.6 3.5 4.4 4.6 2.3 2.8 1.5
Endoplasmic Reticulum 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0
Endosome 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0
Golgi 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0
Mitochondria 0 0 5.0 4.7 – 0 7.5 0 0 0 0 0 0 0 0
Nucleus -1.2 -1.7 -3.1 -1.5 – -2.2 -4.0 -4.3 -4.3 -2.6 -4.0 -4.5 -3.3 -4.0 -2.1
Nuclear Periphery 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0
Vacuole 0 1.9 1.2 0 – 0 0 0 0 0 0 0 2.7 2.3 0

Images

Circle plot not available

Protein Concentration and Protein Localization Data

R1 R2 R3
G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase
Concentration 0.4243 0.558 0.2575 0.2304 -0.036 0.2285 1.5436 1.4143 1.3354 1.6474 1.6363 1.2313 0.822 1.3574 0.9892 0.9589 0.5324 1.0339
Actin 0.0129 0.0044 0 0 0.0046 0.0004 0.0088 0 0.0001 0.004 0.0146 0.0014 0.0015 0 0 0 0 0
Bud 0.0001 0.0001 0 0 0.0005 0 0.0011 0 0.0048 0.0005 0.0003 0.0003 0 0 0 0 0 0
Bud Neck 0.0002 0.0016 0.0001 0 0.0004 0.0008 0.0061 0.0001 0.0001 0.0001 0.0007 0.0009 0.0003 0 0 0 0.0003 0.0004
Bud Periphery 0.0002 0.0002 0 0 0.0012 0 0.0021 0 0.0015 0.0018 0.0004 0.0006 0 0 0 0 0 0
Bud Site 0.0004 0.0083 0 0 0.0016 0.0001 0.0067 0 0.0007 0.001 0.0058 0.0005 0.0012 0 0 0 0 0
Cell Periphery 0.0001 0.0006 0 0 0.0001 0 0.0005 0 0.0001 0.0005 0.0001 0.0001 0 0 0 0 0 0
Cytoplasm 0.0025 0.0053 0.001 0.0003 0.0006 0.0004 0.0056 0.0014 0.0006 0.0131 0.0014 0.001 0.0014 0.0003 0.0003 0 0.0002 0.0002
Cytoplasmic Foci 0.0148 0.004 0 0.0003 0.0074 0.0022 0.0272 0 0.0029 0.0007 0.0105 0.0014 0.0007 0 0 0 0 0
Eisosomes 0.0001 0 0 0 0.0001 0 0.0001 0 0 0.0003 0.0001 0 0 0 0 0 0 0
Endoplasmic Reticulum 0.0007 0.0002 0.0001 0 0.0007 0.0003 0.0023 0.0001 0 0.0013 0.0004 0.0013 0.0001 0 0 0 0 0
Endosome 0.0212 0.0013 0.0001 0 0.0165 0.013 0.0364 0.0001 0.0012 0.0094 0.0101 0.0049 0.0002 0 0 0 0 0
Golgi 0.0069 0.0018 0 0 0.0064 0.0081 0.0095 0 0.0004 0.0019 0.0099 0.0011 0.0001 0 0 0 0 0
Lipid Particles 0.0228 0.0013 0 0 0.0087 0.0002 0.0322 0 0.0051 0.002 0.0311 0.0032 0.0001 0 0 0 0 0
Mitochondria 0.0033 0.0006 0.0002 0.0001 0.095 0.0007 0.0162 0.0001 0.0009 0.0703 0.0458 0.0024 0.0001 0.0001 0.0001 0 0.0001 0
None 0.0023 0.0001 0.002 0.0001 0.0004 0 0.0053 0.0012 0.0007 0.0048 0.0006 0.0006 0.0054 0.0004 0.0051 0 0.0002 0.001
Nuclear Periphery 0.0059 0.0066 0.0081 0.0053 0.0115 0.0018 0.0245 0.0087 0.0017 0.0098 0.015 0.0081 0.0036 0.0024 0.0024 0.0004 0.0008 0.0005
Nucleolus 0.0054 0.0065 0.0055 0.0033 0.0139 0.0183 0.0138 0.0055 0.0059 0.0015 0.0133 0.0194 0.0114 0.0077 0.0062 0.0032 0.0372 0.0123
Nucleus 0.8731 0.931 0.9801 0.9173 0.7996 0.9524 0.7609 0.97 0.9573 0.8707 0.8236 0.9408 0.958 0.9843 0.977 0.9958 0.9405 0.9803
Peroxisomes 0.0177 0.0063 0 0 0.023 0.0001 0.0095 0 0.0019 0.0003 0.008 0.0023 0.0001 0 0 0 0 0
Punctate Nuclear 0.0076 0.0173 0.0026 0.073 0.0049 0.0006 0.017 0.0127 0.0134 0.0012 0.0062 0.0078 0.0154 0.0045 0.0087 0.0004 0.0207 0.0051
Vacuole 0.0011 0.0024 0.0001 0 0.0013 0.0002 0.0099 0.0001 0.0003 0.003 0.0007 0.0014 0.0001 0 0 0 0 0
Vacuole Periphery 0.0005 0.0001 0.0001 0 0.0013 0.0001 0.0043 0.0001 0.0002 0.0018 0.0013 0.0006 0 0 0 0 0 0

Sequencing Data

R1 R2
G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Post-START S/G2 Metaphase Anaphase Telophase
Gene Expression 10.6919 14.8148 8.8655 16.3057 13.8625 10.686 14.1336 15.0018 16.9385 18.1047
Translational Efficiency 0.966 0.7518 1.0255 0.5997 0.7188 1.0602 0.8416 0.5837 0.6431 0.6343

Hit Data

Dataset Hit
Protein Concentration ✘
Protein Localization ✘
Gene Expression ✘
Translational Efficiency ✘

Micrographs

Cell Count

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
1572 518 1561 1407 2206 2010 154 763 3778 2528 1715 2170

Protein Abundance

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
Mean 645.96 811.91 841.45 939.34 765.84 768.91 924.64 816.42 715.96 777.72 848.92 896.12
Standard Deviation 100.58 155.14 105.55 136.67 93.97 109.30 132.57 147.35 113.39 121.37 110.83 152.28
Intensity Change Log 2 — 0.329875 0.381433 0.540203 — 0.005772 0.271849 0.092269 — 0.163137 0.323023 0.314560

Localization Score (DeepLoc)

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
Actin 0.000122 0.001530 0.000633 0.000987 0.000101 0.000270 0.001152 0.001798 0.000110 0.000528 0.000680 0.001272
Bud Neck 0.002666 0.004979 0.014363 0.033653 0.003641 0.006227 0.003586 0.014943 0.003235 0.005971 0.013395 0.027074
Bud Site 0.001700 0.004696 0.001965 0.011551 0.000535 0.001432 0.000689 0.019317 0.001020 0.002101 0.001851 0.014281
Cell Periphery 0.000206 0.000175 0.000079 0.000170 0.000102 0.000122 0.000148 0.000995 0.000145 0.000133 0.000085 0.000460
Cytoplasm 0.034017 0.010460 0.002747 0.014745 0.002485 0.006447 0.002015 0.086007 0.015605 0.007270 0.002681 0.039801
Cytoplasmic Foci 0.003163 0.004214 0.000028 0.003714 0.000405 0.002771 0.000069 0.011658 0.001553 0.003067 0.000032 0.006507
Eisosomes 0.000016 0.000034 0.000022 0.000025 0.000010 0.000017 0.000043 0.000721 0.000012 0.000020 0.000024 0.000270
Endoplasmic Reticulum 0.001547 0.007347 0.005326 0.008286 0.003177 0.004117 0.008669 0.004053 0.002499 0.004779 0.005626 0.006797
Endosome 0.000347 0.001574 0.000167 0.003288 0.000234 0.001824 0.000259 0.005909 0.000281 0.001773 0.000175 0.004210
Golgi 0.000135 0.000802 0.000048 0.005002 0.000100 0.000794 0.000079 0.002461 0.000114 0.000796 0.000051 0.004108
Lipid Particles 0.000495 0.000825 0.000072 0.000640 0.000322 0.001134 0.000268 0.002163 0.000394 0.001071 0.000090 0.001175
Mitochondria 0.001526 0.005691 0.001204 0.004242 0.001438 0.005051 0.000772 0.004021 0.001474 0.005182 0.001165 0.004164
Mitotic Spindle 0.000729 0.005216 0.000544 0.035814 0.002298 0.005013 0.000109 0.012287 0.001645 0.005055 0.000505 0.027542
None 0.045011 0.009495 0.001906 0.005848 0.002606 0.005868 0.004212 0.030071 0.020250 0.006611 0.002113 0.014365
Nuclear Periphery 0.000552 0.000877 0.000498 0.004334 0.001069 0.001641 0.000495 0.001047 0.000854 0.001485 0.000498 0.003178
Nuclear Periphery Foci 0.000441 0.000648 0.000083 0.002807 0.000214 0.000375 0.000249 0.000952 0.000308 0.000431 0.000098 0.002155
Nucleolus 0.012761 0.011722 0.005553 0.004542 0.006486 0.009694 0.008847 0.006291 0.009097 0.010109 0.005849 0.005157
Nucleus 0.893163* 0.923814* 0.963327* 0.848733* 0.973635* 0.940857* 0.965408* 0.781681* 0.940152* 0.937365* 0.963514* 0.825157*
Peroxisomes 0.000388 0.003162 0.000255 0.000598 0.000559 0.000988 0.000330 0.001667 0.000488 0.001433 0.000262 0.000974
Vacuole 0.000820 0.002263 0.001081 0.008951 0.000409 0.004276 0.002487 0.010223 0.000580 0.003864 0.001207 0.009398
Vacuole Periphery 0.000195 0.000476 0.000099 0.002072 0.000172 0.001082 0.000113 0.001736 0.000182 0.000958 0.000100 0.001953

Localization Changes (T-score)

R1 R2 R1 & R2
UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14 UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14 UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14
Actin -2.34 -14.42 -17.00 0.37 -8.10 -3.38 -8.99 -2.23 -1.99 -0.20 -3.19 -17.13 -5.38 -3.25 -2.72
Bud Neck -2.83 -11.59 -16.33 -14.31 -8.32 -3.34 -2.22 -7.89 -6.23 -6.18 -4.53 -11.73 -17.89 -15.48 -7.58
Bud Site -1.68 -0.74 -5.45 -2.14 -5.31 -3.11 -2.52 -4.93 -4.60 -4.61 -2.27 -2.47 -7.70 -6.66 -6.69
Cell Periphery 0.73 4.15 2.09 1.19 -3.87 -0.40 -0.45 -6.73 -5.99 -5.08 0.33 2.92 -5.13 -4.23 -7.60
Cytoplasm 7.17 11.92 7.62 -0.86 -7.34 -4.38 0.73 -11.98 -11.05 -12.06 5.94 10.17 -6.46 -10.68 -13.29
Cytoplasmic Foci -0.69 5.27 2.55 1.81 -3.23 -3.91 3.18 -6.61 -3.92 -6.98 -2.33 5.94 -3.53 -0.56 -7.50
Eisosomes -4.24 -4.50 -12.01 -0.44 -8.80 -5.54 -8.35 -1.89 -1.86 -1.71 -5.75 -11.15 -2.08 -1.98 -1.92
Endoplasmic Reticulum -8.28 -14.58 -21.48 -3.44 -10.55 -2.50 -7.75 -6.33 -5.07 3.10 -7.92 -11.13 -18.37 -11.23 -8.42
Endosome -1.30 3.28 -3.96 -0.06 -4.45 -4.15 -1.87 -4.33 -2.28 -4.20 -4.10 2.33 -5.91 -1.39 -6.11
Golgi -1.70 2.78 -2.40 -1.27 -2.51 -3.42 -0.52 -2.05 -1.02 -2.03 -3.78 2.53 -3.09 -1.71 -3.20
Lipid Particles -1.81 4.74 0.11 1.84 -4.09 -2.27 0.10 -2.62 -0.77 -2.93 -2.54 2.50 -2.27 0.80 -4.68
Mitochondria -1.72 0.38 -4.76 0.57 -9.96 -3.51 0.81 -1.98 1.40 -2.78 -3.96 0.46 -5.02 1.32 -7.36
Mitotic Spindle -1.76 -1.11 -7.99 -5.48 -7.79 -1.80 2.30 -4.37 -3.52 -5.10 -2.85 0.77 -8.85 -7.12 -9.12
None 9.80 13.22 12.21 2.65 -5.45 -2.86 -0.91 -5.97 -4.74 -5.40 8.19 12.55 5.33 -2.80 -7.37
Nuclear Periphery -1.48 -0.70 -11.13 -9.36 -11.18 -1.18 0.05 -3.11 -0.87 -2.60 -1.73 1.24 -10.43 -6.17 -12.27
Nuclear Periphery Foci -0.91 3.67 -2.99 -2.26 -3.92 -1.31 -1.44 -2.97 -2.31 -1.87 -1.14 2.64 -4.17 -3.51 -4.81
Nucleolus 0.63 6.14 6.93 3.23 1.10 -3.23 -1.64 0.52 3.50 1.88 -1.09 3.43 5.11 5.11 1.49
Nucleus -2.98 -11.20 4.69 6.67 16.25 7.61 3.63 16.11 12.22 13.93 0.80 -5.37 17.19 15.52 21.23
Peroxisomes -2.56 1.06 0.50 2.63 -0.60 -1.35 0.81 -1.28 -0.26 -2.17 -3.04 1.42 -0.47 2.81 -2.10
Vacuole -3.36 -2.56 -10.82 -8.83 -10.51 -4.58 -5.57 -7.94 -5.40 -5.81 -4.96 -6.34 -13.52 -9.08 -12.65
Vacuole Periphery -2.33 2.08 -2.07 -1.20 -2.35 -3.90 0.76 -2.36 -0.11 -2.45 -4.16 2.34 -3.04 -0.08 -3.33
Standard name
Human Ortholog
Description Member of the NineTeen Complex (NTC); this complex contains Prp19p and stabilizes U6 snRNA in catalytic forms of the spliceosome containing U2, U5, and U6 snRNAs; homolog of Drosophila crooked neck protein; interacts with U1 snRNP proteins
Localization
Cell Percentages nucleus (97%)
Cell Cycle Regulation No
Subcompartmental Group nucleus-4

Clf1

Clf1


Nearest neighbours ( genes)

correlation threshold

0.6

1.0

0.80


Biological Process (Gene Ontology)
Molecular Function (Gene Ontology)
Cellular Component (Gene Ontology)

Endocytosis
Temp Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Vph1-GFP)
37℃
RT
Cell Cycle Omics

CYCLoPs (Clf1-GFP)
Gene / Allele Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Sac6-tdTomato)
Gene Images
Gene Images
Images are not yet available
Images are not yet available