Standard name
Human Ortholog
Description Acyl-protein thioesterase responsible for depalmitoylation of Gpa1p; green fluorescent protein (GFP)-fusion protein localizes to both the cytoplasm and nucleus and is induced in response to the DNA-damaging agent MMS
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Micrographs

ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 – – – – – –
Cell Periphery 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cytoplasm 0.96 0.99 0.96 0.94 0.91 0.82 0.73 – 0.59 0.58 0.58 0.56 0.99 0.99 0.98 0.88 0.8 0.91 0.96 0.92 0.9 0.96 0.91 0.85
Endoplasmic Reticulum 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0.07 0 0 0 0 0 0 0
Endosome 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Golgi 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Mitochondria 0 0 0 0 0 0.2 0.06 – 0.33 0.29 0.35 0.35 0 0 0 0 0 0 0 0 0 0 0 0
Nucleus 0 0 0 0 0 0 0 – 0.05 0 0 0.05 0 0 0 0 0 0.06 0 0 0 0 0 0
Nuclear Periphery 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Vac/Vac Membrane 0 0 0 0 0.11 0.07 0.25 – 0.17 0.19 0.2 0.13 0 0 0.05 0.21 0.32 0.2 0 0 0 0 0 0
ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 1 – 0 0 0 0 0 0 0 0 0 0 0 2 0 0 1 3
Bud 0 0 0 1 1 2 0 – 4 5 4 3 0 0 0 0 0 0 0 1 0 1 6 5
Bud Neck 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 – 0 0 0 1 0 0 0 0 0 0 – – – – – –
Cell Periphery 0 0 0 0 0 0 6 – 2 0 1 5 0 0 1 0 0 2 0 0 0 0 0 0
Cytoplasm 171 159 53 125 183 145 285 – 149 163 156 150 129 290 274 119 33 93 176 157 68 160 235 158
Endoplasmic Reticulum 0 0 0 1 0 2 0 – 1 1 0 5 0 0 0 5 3 3 0 0 0 0 2 3
Endosome 0 0 0 0 1 0 1 – 0 0 2 1 0 0 0 3 1 0 0 1 0 0 0 2
Golgi 0 0 0 0 1 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 1
Mitochondria 3 2 1 4 2 36 24 – 82 82 94 92 1 1 1 6 1 1 1 0 0 0 2 2
Nucleus 1 0 2 3 4 5 16 – 12 12 12 13 1 1 0 4 0 6 0 0 0 0 0 0
Nuclear Periphery 0 0 0 1 0 0 0 – 0 0 1 4 0 0 0 0 0 0 0 0 0 0 0 1
Nucleolus 1 0 0 0 2 0 0 – 0 2 0 1 0 0 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 1 2
Vac/Vac Membrane 6 0 0 4 22 12 99 – 43 54 54 35 0 4 13 29 13 20 2 1 3 1 2 1
Unique Cell Count 179 160 55 133 202 176 390 252 281 270 266 130 293 280 135 41 102 185 170 76 167 258 186
Labelled Cell Count 182 161 56 139 216 202 432 293 319 324 310 131 296 289 166 51 125 185 170 76 167 258 186


Cytoplasm, Nucleus

(Huh et al. Nature 2003 425:686)
Screen WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 AF100 AF140 AF180
Mean Cell GFP Intensity (1e-4) 6.6 7.0 5.7 6.7 7.6 5.2 6.5 – 5.4 5.7 5.8 5.8 7.7 8.1 8.0 8.3 9.5 9.2 7.4 7.7 8.0
Std Deviation (1e-4) 1.0 1.1 0.8 1.5 1.2 1.2 1.5 – 1.2 1.0 1.5 1.2 1.2 1.8 1.3 1.6 2.0 2.0 2.1 2.8 2.6
Intensity Change (Log2) – – – 0.23 0.4 -0.15 0.18 – -0.08 -0.0 0.01 0.01 0.43 0.51 0.49 0.54 0.73 0.68 0.38 0.42 0.48


Localization RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3
Cortical Patches 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0
Bud 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0
Cell Periphery 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0
Cytoplasm -0.7 -1.4 -2.6 -3.8 – -5.3 -5.4 -5.4 -5.6 1.4 1.5 0.7 -1.8 -2.5 -1.2
Endoplasmic Reticulum 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0
Endosome 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0
Golgi 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0
Mitochondria 0 0 3.3 0 – 4.6 4.3 4.9 4.9 0 0 0 0 0 0
Nucleus 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0
Nuclear Periphery 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0
Vacuole 0 0 0 4.2 – 3.3 3.5 3.6 2.8 0 0 0 3.7 4.5 3.5

Images

Circle plot not available

Protein Concentration and Protein Localization Data

R1 R2 R3
G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase
Concentration 2.3258 2.8962 2.3333 2.4984 2.1161 2.3793 2.9818 4.7354 4.1078 4.2102 3.2224 4.1141 4.8113 6.2424 5.3232 5.3999 5.0284 5.3539
Actin 0.0329 0.0005 0.0044 0.001 0.0448 0.0016 0.0175 0.0017 0.0036 0.0078 0.0004 0.0079 0.0013 0.0003 0.0011 0.0002 0.0006 0.0002
Bud 0.0005 0.0002 0.0003 0.0003 0.0018 0.0005 0.0004 0.0014 0.0013 0.0067 0.0001 0.0007 0.0001 0.0001 0.0004 0.0001 0.0001 0.0001
Bud Neck 0.0067 0.0002 0.0019 0.0004 0.0019 0.0016 0.0061 0.0007 0.0008 0.0019 0.0005 0.0014 0.0003 0.0002 0.0003 0.0003 0.0008 0.0008
Bud Periphery 0.0009 0.0001 0.0003 0.0003 0.0025 0.0004 0.0004 0.0006 0.0007 0.0079 0 0.0004 0 0 0.0003 0 0 0
Bud Site 0.0025 0.0014 0.0008 0.0005 0.0064 0.0008 0.0033 0.0071 0.004 0.0049 0.0001 0.0002 0.0003 0.0011 0.0017 0.0001 0.0001 0
Cell Periphery 0.0002 0.0001 0.0001 0 0.0003 0 0.0001 0.0001 0.0001 0.0003 0 0 0.0001 0.0001 0.0001 0 0 0
Cytoplasm 0.7721 0.9194 0.8191 0.8547 0.7796 0.8635 0.737 0.9507 0.9182 0.7648 0.8865 0.9011 0.8881 0.9366 0.9504 0.9483 0.8736 0.9317
Cytoplasmic Foci 0.0302 0.0057 0.0169 0.0099 0.0202 0.0196 0.0303 0.0037 0.008 0.0267 0.0099 0.0109 0.005 0.0025 0.0041 0.0024 0.0137 0.0033
Eisosomes 0.0002 0 0 0 0.0001 0 0.0002 0 0 0.0001 0 0 0 0 0 0 0 0
Endoplasmic Reticulum 0.0057 0.0048 0.008 0.0029 0.0065 0.0022 0.004 0.0015 0.0022 0.0019 0.0011 0.002 0.004 0.0016 0.0022 0.0016 0.0028 0.0017
Endosome 0.0257 0.0037 0.0247 0.0117 0.0264 0.0087 0.0584 0.006 0.0042 0.0472 0.0129 0.0124 0.0051 0.0005 0.0011 0.0009 0.0089 0.0016
Golgi 0.0069 0.0002 0.003 0.0038 0.0125 0.0018 0.0173 0.0013 0.0048 0.0357 0.007 0.0047 0.0005 0.0001 0.0004 0 0.001 0.0001
Lipid Particles 0.0071 0.0002 0.0056 0.0012 0.0097 0.0077 0.014 0.0001 0.001 0.0196 0.037 0.0059 0.0016 0 0.0001 0 0.0134 0
Mitochondria 0.0136 0.0001 0.0026 0.0318 0.0114 0.0032 0.0162 0.0011 0.0093 0.01 0.0015 0.0086 0.0002 0.0001 0.0001 0.0001 0.0003 0.0001
None 0.0519 0.0375 0.0652 0.0559 0.0094 0.0319 0.0285 0.0161 0.0135 0.0294 0.0058 0.0194 0.0713 0.0426 0.0224 0.0248 0.0523 0.0338
Nuclear Periphery 0.0053 0.0035 0.0072 0.0019 0.0061 0.0021 0.0051 0.0009 0.0076 0.0018 0.0016 0.0022 0.0024 0.001 0.0012 0.0012 0.005 0.0015
Nucleolus 0.0003 0.0001 0.0004 0.0001 0.0009 0.0005 0.0009 0 0.0003 0.0002 0.0001 0.0001 0.0001 0 0 0 0.0002 0
Nucleus 0.0122 0.0194 0.0206 0.012 0.0212 0.0367 0.0337 0.005 0.0105 0.0059 0.024 0.0171 0.0165 0.0118 0.0121 0.0187 0.0109 0.0238
Peroxisomes 0.0101 0.0001 0.0081 0.0075 0.0234 0.012 0.0105 0.0001 0.0055 0.0204 0.0091 0.0017 0.0004 0 0.0001 0 0.0009 0
Punctate Nuclear 0.0102 0.0013 0.003 0.0019 0.0024 0.0036 0.0086 0.0008 0.0021 0.0029 0.0008 0.0024 0.002 0.0007 0.0011 0.0008 0.0142 0.0006
Vacuole 0.0041 0.0015 0.0047 0.0014 0.0101 0.0011 0.0062 0.0008 0.001 0.0034 0.0014 0.0008 0.0006 0.0006 0.0004 0.0003 0.0007 0.0006
Vacuole Periphery 0.001 0.0002 0.0032 0.0005 0.0025 0.0004 0.0012 0.0003 0.0012 0.0008 0.0003 0.0003 0.0001 0 0.0001 0.0001 0.0002 0.0001

Sequencing Data

R1 R2
G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Post-START S/G2 Metaphase Anaphase Telophase
Gene Expression 35.4896 17.3048 34.2425 40.0003 37.6477 40.3068 32.0777 37.888 40.0681 43.1392
Translational Efficiency 1.452 2.5421 1.2676 1.2021 1.3156 1.445 1.7192 1.3249 1.5088 1.2928

Hit Data

Dataset Hit
Protein Concentration ✘
Protein Localization ✘
Gene Expression ✘
Translational Efficiency ✘

Micrographs

Cell Count

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
2483 1107 453 1493 1604 2842 160 1682 4087 3949 613 3175

Protein Abundance

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
Mean 763.34 815.92 1170.30 1068.60 776.78 899.20 1227.60 1138.61 768.61 875.85 1185.26 1105.69
Standard Deviation 93.00 136.96 137.59 162.56 87.09 119.38 159.79 149.05 90.96 130.05 145.90 159.42
Intensity Change Log 2 — 0.096102 0.616481 0.485324 — 0.211136 0.660263 0.551696 — 0.155267 0.638729 0.519181

Localization Score (DeepLoc)

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
Actin 0.000287 0.000959 0.000687 0.004174 0.000239 0.000203 0.001114 0.000968 0.000268 0.000415 0.000798 0.002475
Bud Neck 0.013689 0.005993 0.003258 0.010524 0.003813 0.009830 0.003402 0.006640 0.009813 0.008755 0.003296 0.008467
Bud Site 0.006165 0.017493 0.014345 0.049296 0.004542 0.009883 0.009507 0.027588 0.005528 0.012016 0.013082 0.037796
Cell Periphery 0.000134 0.000122 0.000186 0.000226 0.000116 0.000046 0.000267 0.000120 0.000127 0.000067 0.000207 0.000170
Cytoplasm 0.561114* 0.668551* 0.384891* 0.480754* 0.632004* 0.696643* 0.359946* 0.565513* 0.588936* 0.688768* 0.378380* 0.525657*
Cytoplasmic Foci 0.170072 0.089601 0.007846 0.010517 0.138074 0.045694 0.004800 0.009889 0.157514 0.058002 0.007051 0.010184
Eisosomes 0.000127 0.000037 0.000032 0.000031 0.000066 0.000018 0.000064 0.000021 0.000103 0.000023 0.000040 0.000026
Endoplasmic Reticulum 0.001245 0.001608 0.004566 0.002548 0.001682 0.000374 0.008754 0.001509 0.001417 0.000720 0.005659 0.001998
Endosome 0.005545 0.008133 0.002380 0.010401 0.005117 0.003793 0.003375 0.003426 0.005377 0.005010 0.002640 0.006706
Golgi 0.002120 0.001224 0.000146 0.011789 0.001142 0.001219 0.000160 0.003605 0.001736 0.001220 0.000149 0.007453
Lipid Particles 0.003682 0.001743 0.001447 0.000740 0.003395 0.000831 0.002182 0.000622 0.003570 0.001087 0.001639 0.000677
Mitochondria 0.002870 0.000994 0.001684 0.005883 0.001362 0.002605 0.000491 0.004111 0.002278 0.002154 0.001373 0.004944
Mitotic Spindle 0.001613 0.006177 0.002583 0.082418 0.001328 0.010865 0.002327 0.042335 0.001501 0.009551 0.002516 0.061183
None 0.004224 0.004924 0.006216 0.010598 0.003024 0.004174 0.005112 0.009953 0.003753 0.004384 0.005928 0.010256
Nuclear Periphery 0.000279 0.000312 0.001940 0.001205 0.000250 0.000332 0.001939 0.001267 0.000268 0.000326 0.001940 0.001238
Nuclear Periphery Foci 0.000430 0.002004 0.005447 0.002346 0.000900 0.000202 0.007551 0.001826 0.000614 0.000707 0.005996 0.002070
Nucleolus 0.000986 0.000666 0.000188 0.000517 0.000482 0.000681 0.000222 0.000276 0.000789 0.000677 0.000197 0.000389
Nucleus 0.180912* 0.099843 0.448683* 0.223939* 0.162171* 0.158483* 0.387665* 0.248064* 0.173557* 0.142045* 0.432756* 0.236720*
Peroxisomes 0.002758 0.003265 0.000125 0.002086 0.001676 0.001913 0.000165 0.001087 0.002333 0.002292 0.000136 0.001557
Vacuole 0.040998 0.084423 0.113093 0.081214 0.038233 0.051519 0.200793* 0.067059 0.039913 0.060743 0.135984 0.073715
Vacuole Periphery 0.000749 0.001929 0.000257 0.008792 0.000383 0.000690 0.000165 0.004122 0.000606 0.001037 0.000233 0.006318

Localization Changes (T-score)

R1 R2 R1 & R2
UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14 UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14 UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14
Actin -1.55 -3.80 -2.54 -0.69 -1.21 0.30 -6.62 -2.51 -3.44 4.44 -0.98 -6.39 -3.20 -2.33 0.13
Bud Neck 7.99 8.93 7.91 0.06 -0.74 -7.03 -1.86 -2.66 4.82 -0.33 1.41 7.10 6.61 4.53 -0.62
Bud Site -6.97 -1.05 -5.15 -0.05 -2.69 -4.18 -2.19 -3.93 -0.46 -0.53 -6.73 -1.78 -6.36 -1.27 -2.19
Cell Periphery 1.19 -4.97 -0.81 -1.76 3.93 12.68 -6.67 1.90 -13.44 7.20 10.51 -7.90 0.52 -9.35 8.10
Cytoplasm -8.72 24.26 19.77 23.54 -9.36 -7.57 23.76 24.17 34.13 -12.02 -15.56 33.58 29.69 43.42 -14.97
Cytoplasmic Foci 15.25 42.19 42.46 21.88 0.05 21.42 32.85 32.78 23.28 -1.07 30.86 52.95 53.39 31.41 -0.09
Eisosomes 9.25 8.57 10.19 2.77 4.22 16.50 -2.57 13.09 -7.30 9.59 13.76 7.73 12.76 -4.40 9.39
Endoplasmic Reticulum -2.33 -11.14 -4.87 -3.16 8.74 2.73 -7.92 -0.04 -14.85 8.99 3.11 -12.79 -2.51 -13.47 12.86
Endosome -3.18 6.78 5.10 6.69 0.31 3.24 3.32 10.27 7.50 6.38 1.43 7.76 10.25 8.33 3.60
Golgi 4.27 11.76 -0.29 -1.62 -2.91 -0.08 4.78 1.23 1.10 -1.76 2.30 12.25 0.64 -0.75 -3.39
Lipid Particles 5.08 6.59 10.16 1.86 4.63 6.21 1.16 5.63 -2.70 7.20 10.32 5.82 11.09 -0.91 8.06
Mitochondria 5.18 3.14 2.47 -2.52 -1.22 -1.53 1.30 -0.00 2.06 -2.75 0.42 2.85 1.97 1.23 -1.46
Mitotic Spindle -3.18 -2.20 -8.45 -6.57 -6.26 -6.30 -1.70 -8.14 -4.08 -1.83 -7.07 -2.75 -11.73 -6.95 -6.41
None -0.34 -0.40 -5.09 -4.54 -5.03 -2.56 -0.23 -13.47 -12.42 -11.52 -1.09 -0.86 -11.22 -11.22 -10.47
Nuclear Periphery -1.40 -20.18 -22.56 -19.81 5.36 -4.94 -13.96 -22.54 -20.91 4.34 -3.74 -24.54 -31.41 -29.63 6.99
Nuclear Periphery Foci -7.71 -7.47 -9.29 1.98 5.39 8.56 -6.29 -5.10 -10.86 5.55 -2.11 -9.48 -10.65 -7.85 7.46
Nucleolus 2.40 5.64 5.02 4.15 -1.03 -2.36 3.73 0.96 2.97 -1.77 1.28 6.39 4.78 4.68 -1.93
Nucleus 14.56 -22.70 -18.97 -29.00 12.25 0.26 -10.64 -24.31 -26.00 2.94 7.64 -25.04 -30.45 -36.16 11.77
Peroxisomes -0.59 13.04 7.98 4.94 -1.76 -0.57 5.21 3.90 4.55 -0.76 0.37 13.17 8.93 6.43 -1.84
Vacuole -13.45 -13.82 -26.37 -13.31 -1.92 -7.56 -12.92 -24.30 -20.42 6.47 -14.14 -18.28 -35.75 -26.34 2.78
Vacuole Periphery -1.75 6.74 -1.76 -0.11 -2.71 -3.09 3.72 -1.20 -0.37 -1.82 -2.09 7.27 -1.98 -0.83 -3.24
Standard name
Human Ortholog
Description Acyl-protein thioesterase responsible for depalmitoylation of Gpa1p; green fluorescent protein (GFP)-fusion protein localizes to both the cytoplasm and nucleus and is induced in response to the DNA-damaging agent MMS
Localization
Cell Percentages cytoplasm (85%)
Cell Cycle Regulation No
Subcompartmental Group cytoplasm-9

Tml25

Tml25


Nearest neighbours ( genes)

correlation threshold

0.6

1.0

0.80


Biological Process (Gene Ontology)
Molecular Function (Gene Ontology)
Cellular Component (Gene Ontology)

Endocytosis
Temp Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Vph1-GFP)
37℃
RT
Cell Cycle Omics

CYCLoPs (Tml25-GFP)
Gene / Allele Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Sac6-tdTomato)
Gene Images
Gene Images
Images are not yet available
Images are not yet available