Standard name | |
---|---|
Human Ortholog | |
Description | Aspartic protease; member of the yapsin family of proteases involved in cell wall growth and maintenance; attached to the plasma membrane via a glycosylphosphatidylinositol (GPI) anchor |
ensLOC | DeepLoc | |||||||||||||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Localization | WT1 | WT2 | WT3 | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 | WT1 | WT2 | WT3 | AF100 | AF140 | AF180 |
Cortical Patches | 0 | 5 | 1 | 0 | 0 | 0 | 2 | 0 | 2 | 2 | 0 | 1 | 1 | 0 | 0 | 13 | 3 | 0 | 3 | 15 | 7 | 3 | 6 | 15 |
Bud | 2 | 10 | 6 | 7 | 3 | 3 | 4 | 8 | 5 | 16 | 8 | 15 | 1 | 1 | 1 | 4 | 0 | 1 | 3 | 12 | 5 | 4 | 8 | 9 |
Bud Neck | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 2 | 6 | 2 | 0 | 6 | 9 |
Bud Site | 0 | 1 | 0 | 0 | 0 | 2 | 4 | 2 | 3 | 4 | 1 | 2 | 0 | 0 | 0 | 0 | 0 | 0 | – | – | – | – | – | – |
Cell Periphery | 1 | 8 | 4 | 1 | 3 | 3 | 0 | 1 | 2 | 1 | 0 | 2 | 2 | 2 | 2 | 13 | 3 | 0 | 0 | 3 | 1 | 0 | 1 | 2 |
Cytoplasm | 24 | 89 | 49 | 21 | 32 | 22 | 15 | 27 | 11 | 24 | 17 | 22 | 68 | 114 | 117 | 35 | 12 | 4 | 5 | 23 | 8 | 4 | 14 | 3 |
Endoplasmic Reticulum | 4 | 15 | 5 | 0 | 1 | 2 | 5 | 10 | 6 | 7 | 5 | 4 | 4 | 6 | 3 | 6 | 2 | 0 | 2 | 11 | 3 | 2 | 4 | 12 |
Endosome | 4 | 22 | 3 | 9 | 21 | 11 | 7 | 23 | 1 | 1 | 0 | 2 | 5 | 3 | 14 | 26 | 6 | 2 | 9 | 35 | 17 | 4 | 11 | 11 |
Golgi | 2 | 10 | 1 | 0 | 7 | 1 | 2 | 2 | 1 | 0 | 0 | 1 | 5 | 2 | 2 | 20 | 13 | 2 | 4 | 14 | 3 | 1 | 7 | 9 |
Mitochondria | 86 | 157 | 80 | 57 | 81 | 148 | 223 | 201 | 193 | 206 | 143 | 245 | 55 | 55 | 38 | 26 | 2 | 2 | 84 | 220 | 106 | 45 | 81 | 58 |
Nucleus | 1 | 0 | 0 | 0 | 0 | 3 | 7 | 5 | 2 | 1 | 0 | 4 | 0 | 1 | 0 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Nuclear Periphery | 0 | 5 | 0 | 0 | 0 | 2 | 13 | 7 | 6 | 5 | 3 | 7 | 0 | 0 | 0 | 0 | 0 | 1 | 0 | 0 | 1 | 0 | 1 | 1 |
Nucleolus | 1 | 0 | 1 | 0 | 4 | 12 | 9 | 12 | 8 | 14 | 6 | 12 | 0 | 0 | 0 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Peroxisomes | 1 | 2 | 1 | 4 | 2 | 4 | 2 | 6 | 2 | 2 | 2 | 0 | 3 | 6 | 2 | 10 | 1 | 1 | 1 | 2 | 4 | 0 | 1 | 2 |
SpindlePole | 1 | 4 | 3 | 1 | 3 | 4 | 7 | 9 | 2 | 2 | 1 | 4 | 0 | 3 | 0 | 5 | 4 | 0 | 3 | 10 | 4 | 0 | 3 | 2 |
Vac/Vac Membrane | 11 | 52 | 6 | 3 | 29 | 34 | 26 | 43 | 16 | 13 | 11 | 17 | 15 | 11 | 14 | 32 | 10 | 6 | 10 | 50 | 6 | 3 | 8 | 9 |
Unique Cell Count | 113 | 315 | 144 | 80 | 152 | 203 | 273 | 288 | 214 | 241 | 168 | 277 | 136 | 181 | 170 | 153 | 45 | 18 | 133 | 413 | 177 | 76 | 165 | 150 |
Labelled Cell Count | 138 | 380 | 160 | 103 | 186 | 251 | 326 | 356 | 260 | 298 | 197 | 338 | 159 | 204 | 193 | 192 | 56 | 19 | 133 | 413 | 177 | 76 | 165 | 150 |
Screen | WT1 | WT2 | WT3 | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 | AF100 | AF140 | AF180 |
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Mean Cell GFP Intensity (1e-4) | 4.0 | 5.0 | 5.5 | 4.6 | 6.4 | 6.1 | 5.5 | 6.3 | 5.6 | 5.3 | 5.4 | 5.3 | 5.2 | 5.1 | 5.4 | 7.7 | 9.0 | 11.9 | 4.5 | 5.5 | 7.6 |
Std Deviation (1e-4) | 1.0 | 1.2 | 3.5 | 1.3 | 2.3 | 2.8 | 2.1 | 2.3 | 1.8 | 2.1 | 1.8 | 1.8 | 1.3 | 1.6 | 2.0 | 3.1 | 3.2 | 7.9 | 1.0 | 1.7 | 2.8 |
Intensity Change (Log2) | – | – | – | -0.26 | 0.22 | 0.17 | 0.01 | 0.2 | 0.03 | -0.04 | -0.02 | -0.04 | -0.08 | -0.1 | -0.01 | 0.49 | 0.72 | 1.12 | -0.3 | 0.0 | 0.47 |
Localization | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 |
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Cortical Patches | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 3.2 | 0 | 0 |
Bud | 0 | 0 | 0 | 0 | 0 | 0 | 1.0 | 0.3 | 0.6 | 0 | 0 | 0 | 0 | 0 | 0 |
Bud Neck | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Bud Site | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Cell Periphery | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 2.1 | 0 | 0 |
Cytoplasm | -1.2 | -2.5 | -5.3 | -7.7 | -6.3 | -7.2 | -5.8 | -5.2 | -6.8 | 2.7 | 5.2 | 6.2 | -2.1 | -0.9 | -1.0 |
Endoplasmic Reticulum | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0.2 | 0 | 0 |
Endosome | 0 | 3.7 | 1.6 | 0 | 2.4 | 0 | 0 | 0 | 0 | 0 | 0 | 2.4 | 4.3 | 0 | 0 |
Golgi | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 4.2 | 0 | 0 |
Mitochondria | 0 | -0.4 | 3.4 | 5.7 | 2.9 | 7.6 | 6.5 | 5.8 | 7.6 | -2.5 | -4.6 | -6.1 | -6.9 | -6.0 | -3.6 |
Nucleus | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Nuclear Periphery | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Nucleolus | 0 | 0 | 2.5 | 0 | 0 | 0 | 2.5 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Peroxisomes | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 2.7 | 0 | 0 |
SpindlePole | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Vacuole | 0 | 4.0 | 3.6 | 2.0 | 3.3 | 1.3 | 0.5 | 0.9 | 0.8 | 2.2 | 0.8 | 1.5 | 4.3 | 0 | 0 |
R1 | R2 | R3 | ||||||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | |
Concentration | -0.1215 | 1.8092 | 1.3501 | 0.8484 | 0.551 | 1.1315 | 2.1851 | 2.6058 | 2.833 | 3.1291 | 3.257 | 2.5248 | 3.4371 | 2.9664 | 3.348 | 2.5406 | 3.0511 | 3.0313 |
Actin | 0.0504 | 0.0281 | 0.0355 | 0.0121 | 0.1506 | 0.0254 | 0.0546 | 0.0197 | 0.0569 | 0.0149 | 0.0234 | 0.0201 | 0.0977 | 0.0184 | 0.0573 | 0.0437 | 0.0436 | 0.0243 |
Bud | 0.0068 | 0.0034 | 0.0072 | 0.0025 | 0.0069 | 0.0036 | 0.0047 | 0.0167 | 0.0094 | 0.0019 | 0.0074 | 0.0036 | 0.0044 | 0.0211 | 0.0125 | 0.0033 | 0.0117 | 0.0054 |
Bud Neck | 0.0032 | 0.0056 | 0.008 | 0.0008 | 0.0029 | 0.0141 | 0.0029 | 0.0076 | 0.0189 | 0.0034 | 0.0021 | 0.0124 | 0.0054 | 0.0035 | 0.0082 | 0.0019 | 0.0502 | 0.0234 |
Bud Periphery | 0.0346 | 0.0078 | 0.0214 | 0.0078 | 0.0191 | 0.0078 | 0.0169 | 0.0351 | 0.0228 | 0.0033 | 0.02 | 0.0135 | 0.0366 | 0.0199 | 0.0233 | 0.0081 | 0.0607 | 0.0226 |
Bud Site | 0.0304 | 0.1233 | 0.0756 | 0.0052 | 0.0177 | 0.004 | 0.0165 | 0.0868 | 0.0345 | 0.0111 | 0.0035 | 0.0035 | 0.0082 | 0.1188 | 0.0366 | 0.0149 | 0.0068 | 0.0019 |
Cell Periphery | 0.0049 | 0.0198 | 0.0133 | 0.0025 | 0.0043 | 0.0017 | 0.0036 | 0.0033 | 0.0049 | 0.0011 | 0.0014 | 0.0019 | 0.0139 | 0.0282 | 0.0218 | 0.0015 | 0.0037 | 0.0054 |
Cytoplasm | 0.1139 | 0.1386 | 0.1268 | 0.0785 | 0.0693 | 0.1306 | 0.1009 | 0.1691 | 0.1382 | 0.0695 | 0.0872 | 0.1248 | 0.1507 | 0.1541 | 0.1196 | 0.1236 | 0.0859 | 0.1529 |
Cytoplasmic Foci | 0.0344 | 0.049 | 0.0611 | 0.0943 | 0.0567 | 0.0897 | 0.0961 | 0.0704 | 0.0874 | 0.1376 | 0.1052 | 0.0887 | 0.0782 | 0.0885 | 0.0684 | 0.041 | 0.0775 | 0.071 |
Eisosomes | 0.0013 | 0.0026 | 0.0027 | 0.0012 | 0.0013 | 0.0007 | 0.0014 | 0.001 | 0.0012 | 0.0011 | 0.0005 | 0.0005 | 0.0017 | 0.0016 | 0.0076 | 0.0007 | 0.0017 | 0.0014 |
Endoplasmic Reticulum | 0.0225 | 0.0304 | 0.032 | 0.0391 | 0.0123 | 0.0133 | 0.012 | 0.0079 | 0.0048 | 0.0022 | 0.0033 | 0.0089 | 0.0177 | 0.0089 | 0.0095 | 0.0027 | 0.0096 | 0.0047 |
Endosome | 0.0456 | 0.0959 | 0.0665 | 0.1182 | 0.0908 | 0.1249 | 0.1724 | 0.1073 | 0.089 | 0.0999 | 0.1514 | 0.0684 | 0.1086 | 0.1077 | 0.0469 | 0.0566 | 0.0894 | 0.0852 |
Golgi | 0.0183 | 0.04 | 0.032 | 0.0247 | 0.0475 | 0.0633 | 0.078 | 0.0355 | 0.0601 | 0.0383 | 0.1268 | 0.0417 | 0.0379 | 0.0413 | 0.0376 | 0.0325 | 0.0455 | 0.0415 |
Lipid Particles | 0.0275 | 0.0121 | 0.0242 | 0.0132 | 0.0324 | 0.0207 | 0.037 | 0.005 | 0.0112 | 0.0079 | 0.0684 | 0.0133 | 0.0234 | 0.0098 | 0.0165 | 0.007 | 0.014 | 0.0161 |
Mitochondria | 0.1463 | 0.218 | 0.1623 | 0.2242 | 0.3243 | 0.2137 | 0.1062 | 0.13 | 0.1897 | 0.3 | 0.1838 | 0.2036 | 0.0596 | 0.1591 | 0.2585 | 0.3711 | 0.166 | 0.2204 |
None | 0.4216 | 0.1828 | 0.2621 | 0.3193 | 0.1026 | 0.2482 | 0.2278 | 0.2682 | 0.2262 | 0.2753 | 0.093 | 0.3437 | 0.2797 | 0.1665 | 0.2267 | 0.2651 | 0.2968 | 0.2995 |
Nuclear Periphery | 0.0076 | 0.0056 | 0.0041 | 0.0039 | 0.0055 | 0.0034 | 0.0039 | 0.0013 | 0.0014 | 0.0006 | 0.0007 | 0.0038 | 0.0159 | 0.0014 | 0.0014 | 0.0009 | 0.0026 | 0.0018 |
Nucleolus | 0.0018 | 0.0007 | 0.0022 | 0.0006 | 0.0014 | 0.0006 | 0.0011 | 0.0017 | 0.001 | 0.0013 | 0.0005 | 0.0005 | 0.001 | 0.0017 | 0.0014 | 0.0006 | 0.0033 | 0.0005 |
Nucleus | 0.0029 | 0.0024 | 0.0031 | 0.0018 | 0.0029 | 0.0015 | 0.0025 | 0.0021 | 0.0015 | 0.002 | 0.0008 | 0.0013 | 0.0042 | 0.0021 | 0.002 | 0.0013 | 0.0029 | 0.001 |
Peroxisomes | 0.009 | 0.0025 | 0.0222 | 0.0265 | 0.0195 | 0.0151 | 0.0446 | 0.0147 | 0.0235 | 0.0151 | 0.0938 | 0.0318 | 0.0192 | 0.014 | 0.0243 | 0.0098 | 0.0117 | 0.0118 |
Punctate Nuclear | 0.0044 | 0.001 | 0.0022 | 0.001 | 0.0029 | 0.0022 | 0.005 | 0.0011 | 0.0011 | 0.0013 | 0.0011 | 0.0021 | 0.0158 | 0.0008 | 0.0012 | 0.0005 | 0.0058 | 0.0008 |
Vacuole | 0.0089 | 0.0215 | 0.0265 | 0.0163 | 0.0222 | 0.0112 | 0.0091 | 0.012 | 0.0131 | 0.0099 | 0.019 | 0.0073 | 0.0123 | 0.0292 | 0.0147 | 0.0088 | 0.0076 | 0.0055 |
Vacuole Periphery | 0.0038 | 0.0088 | 0.0088 | 0.0064 | 0.0065 | 0.0044 | 0.0029 | 0.0035 | 0.0032 | 0.0022 | 0.0066 | 0.0043 | 0.0078 | 0.0035 | 0.004 | 0.0046 | 0.003 | 0.0028 |
R1 | R2 | |||||||||
---|---|---|---|---|---|---|---|---|---|---|
G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | |
Gene Expression | 33.2394 | 9.0434 | 2.876 | 9.1536 | 35.1329 | 36.1098 | 9.4703 | 5.951 | 7.4783 | 35.4971 |
Translational Efficiency | 0.9392 | 0.7762 | 0.755 | 0.6637 | 0.8313 | 0.8647 | 0.4876 | 0.6863 | 0.5084 | 0.8076 |
Dataset | Hit |
---|---|
Protein Concentration | ✘ |
Protein Localization | ✘ |
Gene Expression | ✔ |
Translational Efficiency | ✘ |
R1 | R2 | R1 & R2 | |||||||||
---|---|---|---|---|---|---|---|---|---|---|---|
WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 |
2049 | 222 | 298 | 2004 | 1917 | 285 | 196 | 2829 | 3966 | 507 | 494 | 4833 |
R1 | R2 | R1 & R2 | ||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|
WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 | |
Mean | 671.59 | 716.09 | 934.84 | 1146.34 | 723.89 | 775.73 | 1022.37 | 1177.14 | 696.87 | 749.62 | 969.57 | 1164.37 |
Standard Deviation | 81.14 | 117.59 | 124.41 | 384.33 | 95.65 | 119.33 | 133.07 | 341.29 | 92.23 | 122.21 | 134.90 | 360.09 |
Intensity Change Log 2 | — | 0.092560 | 0.477139 | 0.771382 | — | 0.099784 | 0.498075 | 0.701444 | — | 0.096312 | 0.488037 | 0.735526 |
R1 | R2 | R1 & R2 | ||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|
WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 | |
Actin | 0.000498 | 0.001842 | 0.007359 | 0.012713 | 0.000519 | 0.001023 | 0.015079 | 0.009240 | 0.000508 | 0.001382 | 0.010422 | 0.010680 |
Bud Neck | 0.067949 | 0.030441 | 0.014307 | 0.006701 | 0.033718 | 0.034427 | 0.014160 | 0.007137 | 0.051403 | 0.032681 | 0.014248 | 0.006956 |
Bud Site | 0.046265 | 0.040972 | 0.043516 | 0.187645* | 0.035264 | 0.047653 | 0.098821 | 0.169807* | 0.040948 | 0.044728 | 0.065459 | 0.177203* |
Cell Periphery | 0.000904 | 0.000402 | 0.000293 | 0.001943 | 0.000534 | 0.000643 | 0.000783 | 0.001762 | 0.000725 | 0.000538 | 0.000488 | 0.001837 |
Cytoplasm | 0.229877* | 0.054153 | 0.184862* | 0.072689 | 0.123813* | 0.061955 | 0.212977* | 0.088862 | 0.178610* | 0.058539 | 0.196017* | 0.082156 |
Cytoplasmic Foci | 0.201066* | 0.178708 | 0.091268 | 0.061127 | 0.197413* | 0.189028* | 0.145981 | 0.051267 | 0.199300* | 0.184509* | 0.112976 | 0.055355 |
Eisosomes | 0.000384 | 0.000186 | 0.000135 | 0.000131 | 0.000188 | 0.000177 | 0.000187 | 0.000111 | 0.000289 | 0.000181 | 0.000156 | 0.000119 |
Endoplasmic Reticulum | 0.003845 | 0.000887 | 0.012712 | 0.003411 | 0.003161 | 0.000906 | 0.011649 | 0.002840 | 0.003514 | 0.000898 | 0.012290 | 0.003076 |
Endosome | 0.055701 | 0.131505 | 0.035629 | 0.059005 | 0.080464 | 0.121099 | 0.053393 | 0.055397 | 0.067671 | 0.125656 | 0.042677 | 0.056893 |
Golgi | 0.021501 | 0.132710* | 0.010597 | 0.162413* | 0.063961 | 0.106339 | 0.034595 | 0.125593* | 0.042024 | 0.117887* | 0.020118 | 0.140860* |
Lipid Particles | 0.031456 | 0.009825 | 0.011248 | 0.000953 | 0.021880 | 0.009172 | 0.010555 | 0.000762 | 0.026827 | 0.009458 | 0.010973 | 0.000842 |
Mitochondria | 0.137542* | 0.243179* | 0.004187 | 0.212588* | 0.277819* | 0.272477* | 0.012249 | 0.276078* | 0.205346* | 0.259648* | 0.007386 | 0.249751* |
Mitotic Spindle | 0.006483 | 0.029086 | 0.051995 | 0.084224 | 0.015104 | 0.021472 | 0.043368 | 0.052473 | 0.010650 | 0.024806 | 0.048572 | 0.065638 |
None | 0.006564 | 0.003463 | 0.003729 | 0.000775 | 0.002366 | 0.000933 | 0.001824 | 0.000841 | 0.004535 | 0.002041 | 0.002973 | 0.000814 |
Nuclear Periphery | 0.001190 | 0.000342 | 0.003066 | 0.000309 | 0.000972 | 0.000228 | 0.001763 | 0.000287 | 0.001085 | 0.000278 | 0.002549 | 0.000296 |
Nuclear Periphery Foci | 0.000773 | 0.001487 | 0.006611 | 0.000848 | 0.000711 | 0.000316 | 0.007396 | 0.000630 | 0.000743 | 0.000829 | 0.006923 | 0.000720 |
Nucleolus | 0.003835 | 0.004036 | 0.002359 | 0.000277 | 0.002621 | 0.002376 | 0.001055 | 0.000177 | 0.003248 | 0.003103 | 0.001841 | 0.000219 |
Nucleus | 0.078201 | 0.017082 | 0.340034* | 0.002199 | 0.035930 | 0.012820 | 0.121386 | 0.001658 | 0.057769 | 0.014686 | 0.253283* | 0.001882 |
Peroxisomes | 0.021926 | 0.060653 | 0.017101 | 0.013440 | 0.030407 | 0.052669 | 0.031632 | 0.006613 | 0.026025 | 0.056165 | 0.022866 | 0.009444 |
Vacuole | 0.069693 | 0.039809 | 0.153705 | 0.071346 | 0.053105 | 0.039620 | 0.171311 | 0.087694 | 0.061675 | 0.039703 | 0.160690 | 0.080915 |
Vacuole Periphery | 0.014349 | 0.019231 | 0.005287 | 0.045263 | 0.020049 | 0.024666 | 0.009838 | 0.060771 | 0.017104 | 0.022286 | 0.007093 | 0.054341 |
R1 | R2 | R1 & R2 | |||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
UBP2_WT | UBP14_WT | UBP2UBP14_WT | UBP2UBP14_UBP2 | UBP2UBP14_UBP14 | UBP2_WT | UBP14_WT | UBP2UBP14_WT | UBP2UBP14_UBP2 | UBP2UBP14_UBP14 | UBP2_WT | UBP14_WT | UBP2UBP14_WT | UBP2UBP14_UBP2 | UBP2UBP14_UBP14 | |
Actin | -1.64 | -2.91 | -10.94 | -7.86 | -3.15 | -2.97 | -3.50 | -10.66 | -9.83 | 0.87 | -2.37 | -4.55 | -15.25 | -12.21 | -1.21 |
Bud Neck | 6.43 | 14.92 | 20.43 | 4.56 | 2.87 | -0.12 | 7.40 | 12.98 | 4.93 | 2.52 | 4.46 | 16.09 | 23.93 | 6.67 | 3.75 |
Bud Site | 0.93 | 1.82 | -24.89 | -19.83 | -20.47 | -1.83 | -4.74 | -28.77* | -15.93 | -8.28 | -0.82 | -2.44 | -37.63 | -24.60 | -20.30 |
Cell Periphery | 4.77 | 6.69 | -4.87 | -7.73 | -8.61 | -0.71 | -0.99 | -4.70 | -3.81 | -3.77 | 1.87 | 3.73 | -6.23 | -6.84 | -7.84 |
Cytoplasm | 18.30 | 3.64 | 23.97 | -2.67 | 9.08 | 7.04 | -5.02 | 5.48 | -3.78 | 6.94 | 18.25 | -1.56 | 21.07 | -4.68 | 11.05 |
Cytoplasmic Foci | 1.72 | 15.93 | 28.76 | 10.46 | 2.34 | 0.67 | 6.28 | 30.75 | 11.72 | 7.40 | 1.60 | 15.69 | 42.62 | 15.70 | 7.12 |
Eisosomes | 1.61 | 2.02 | 2.09 | 3.08 | 0.85 | 0.59 | -0.22 | 7.27 | 3.62 | 4.99 | 1.69 | 2.04 | 2.72 | 4.79 | 4.62 |
Endoplasmic Reticulum | 6.98 | -8.43 | 0.77 | -6.66 | 8.88 | 4.75 | -4.50 | 0.69 | -6.02 | 4.75 | 8.15 | -9.13 | 1.24 | -8.97 | 9.75 |
Endosome | -6.64 | 6.30 | -0.85 | 6.46 | -7.16 | -4.26 | 5.94 | 7.98 | 7.21 | -1.64 | -7.93 | 9.15 | 5.50 | 9.70 | -6.31 |
Golgi | -8.41 | 4.84 | -26.76* | -1.95 | -27.10* | -4.37 | 6.19 | -12.94 | -1.76 | -15.04 | -9.54 | 8.75 | -28.61 | -2.52 | -30.90* |
Lipid Particles | 8.02 | 10.03 | 18.05 | 4.34 | 7.68 | 5.44 | 3.51 | 14.58 | 4.55 | 3.36 | 9.75 | 9.21 | 23.19 | 6.29 | 7.04 |
Mitochondria | -5.14 | 26.25 | -9.06 | 1.60 | -34.10* | 0.25 | 34.57* | 0.51 | -0.01 | -41.25* | -3.82 | 42.88* | -6.71 | 0.91 | -55.38* |
Mitotic Spindle | -3.83 | -4.46 | -20.75 | -7.99 | -4.52 | -1.63 | -3.22 | -14.16 | -7.37 | -2.17 | -4.19 | -5.33 | -25.27 | -10.68 | -4.15 |
None | 1.03 | 2.38 | 9.28 | 1.43 | 1.88 | 3.65 | 1.21 | 5.82 | 0.34 | 1.49 | 2.04 | 2.25 | 10.70 | 1.46 | 2.20 |
Nuclear Periphery | 4.42 | -6.81 | 5.28 | 0.17 | 10.05 | 3.81 | -1.55 | 3.55 | -0.90 | 2.82 | 6.01 | -5.71 | 6.25 | -0.39 | 8.70 |
Nuclear Periphery Foci | -0.66 | -5.45 | -0.40 | 0.58 | 5.32 | 2.89 | -6.45 | 0.38 | -2.32 | 6.51 | -0.23 | -8.13 | 0.07 | 0.24 | 8.11 |
Nucleolus | -0.51 | 3.35 | 9.14 | 2.71 | 6.12 | 0.28 | 3.73 | 6.74 | 2.87 | 4.39 | -0.15 | 4.48 | 11.64 | 3.83 | 8.02 |
Nucleus | 10.11 | -14.72 | 24.45 | 2.80 | 18.88 | 5.94 | -6.39 | 15.09 | 3.44 | 8.71 | 12.33 | -15.24 | 28.43 | 4.34 | 19.41 |
Peroxisomes | -4.91 | 2.57 | 5.04 | 5.99 | 0.33 | -3.54 | 0.50 | 14.20 | 7.58 | 3.82 | -6.13 | 2.23 | 14.35 | 9.61 | 3.66 |
Vacuole | 4.94 | -9.70 | -1.74 | -5.99 | 9.08 | 2.87 | -9.95 | -10.94 | -10.31 | 7.80 | 5.84 | -14.00 | -8.79 | -11.66 | 11.62 |
Vacuole Periphery | -1.79 | 7.98 | -14.11 | -7.97 | -18.27 | -1.35 | 4.37 | -16.89 | -9.09 | -15.62* | -2.28 | 8.42 | -22.40 | -12.04 | -25.21* |
Temp | Actin Patch (Sac6-tdTomato) | Cortical Patch (Sla1-GFP) | Late Endosome (Snf7-GFP) | Vacuole (Vph1-GFP) |
---|---|---|---|---|
37℃ | ||||
RT |
Gene / Allele | Actin Patch (Sac6-tdTomato) | Cortical Patch (Sla1-GFP) | Late Endosome (Snf7-GFP) | Vacuole (Sac6-tdTomato) |
---|
Gene | Images |
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Gene | Images |
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