Standard name | |
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Human Ortholog | |
Description | Low-affinity zinc transporter of the plasma membrane; transcription is induced under low-zinc conditions by the Zap1p transcription factor |
ensLOC | DeepLoc | |||||||||||||||||||||||
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Localization | WT1 | WT2 | WT3 | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 | WT1 | WT2 | WT3 | AF100 | AF140 | AF180 |
Cortical Patches | 0 | 1 | 4 | 5 | 2 | 0 | 0 | 1 | – | 1 | 1 | 1 | 8 | 8 | 3 | 1 | 3 | 4 | 0 | 1 | 7 | 1 | 9 | 6 |
Bud | 0 | 2 | 2 | 2 | 1 | 4 | 6 | 10 | – | 18 | 26 | 18 | 1 | 1 | 0 | 0 | 0 | 1 | 0 | 1 | 3 | 3 | 11 | 10 |
Bud Neck | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 2 | – | 0 | 0 | 2 | 1 | 1 | 5 | 1 | 0 | 0 | 0 | 1 | 1 | 1 | 6 | 4 |
Bud Site | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | – | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | – | – | – | – | – | – |
Cell Periphery | 0 | 0 | 0 | 4 | 1 | 0 | 1 | 2 | – | 2 | 0 | 6 | 16 | 16 | 21 | 1 | 3 | 1 | 0 | 0 | 0 | 0 | 1 | 0 |
Cytoplasm | 23 | 93 | 237 | 270 | 532 | 672 | 943 | 972 | – | 979 | 524 | 845 | 392 | 392 | 461 | 65 | 177 | 163 | 5 | 15 | 18 | 24 | 63 | 61 |
Endoplasmic Reticulum | 12 | 50 | 165 | 130 | 23 | 2 | 5 | 2 | – | 3 | 2 | 5 | 167 | 181 | 162 | 0 | 8 | 6 | 50 | 104 | 358 | 219 | 347 | 251 |
Endosome | 0 | 1 | 13 | 5 | 17 | 4 | 4 | 12 | – | 6 | 2 | 7 | 1 | 1 | 2 | 0 | 5 | 4 | 0 | 3 | 9 | 3 | 6 | 8 |
Golgi | 0 | 0 | 0 | 0 | 1 | 0 | 0 | 0 | – | 0 | 1 | 0 | 1 | 1 | 1 | 0 | 0 | 0 | 0 | 0 | 2 | 0 | 2 | 1 |
Mitochondria | 1 | 1 | 1 | 1 | 2 | 28 | 53 | 70 | – | 119 | 110 | 161 | 0 | 0 | 0 | 6 | 7 | 7 | 2 | 3 | 9 | 18 | 25 | 21 |
Nucleus | 1 | 0 | 1 | 0 | 1 | 1 | 5 | 2 | – | 1 | 3 | 5 | 2 | 2 | 3 | 1 | 2 | 3 | 0 | 0 | 0 | 0 | 0 | 0 |
Nuclear Periphery | 0 | 1 | 3 | 4 | 0 | 1 | 3 | 7 | – | 3 | 2 | 16 | 0 | 0 | 0 | 0 | 0 | 1 | 0 | 2 | 4 | 2 | 3 | 4 |
Nucleolus | 0 | 0 | 0 | 0 | 1 | 1 | 0 | 4 | – | 1 | 2 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Peroxisomes | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
SpindlePole | 0 | 0 | 0 | 0 | 0 | 1 | 1 | 4 | – | 5 | 0 | 9 | 0 | 0 | 0 | 0 | 0 | 1 | 0 | 0 | 0 | 1 | 1 | 3 |
Vac/Vac Membrane | 1 | 26 | 85 | 48 | 85 | 63 | 76 | 64 | – | 37 | 23 | 51 | 56 | 53 | 27 | 4 | 28 | 25 | 33 | 77 | 113 | 91 | 169 | 293 |
Unique Cell Count | 38 | 157 | 464 | 422 | 609 | 714 | 1014 | 1066 | 1063 | 602 | 997 | 593 | 602 | 639 | 72 | 218 | 198 | 95 | 214 | 534 | 373 | 655 | 676 | |
Labelled Cell Count | 38 | 175 | 511 | 469 | 666 | 777 | 1097 | 1152 | 1176 | 696 | 1126 | 645 | 656 | 685 | 79 | 233 | 216 | 95 | 214 | 534 | 373 | 655 | 676 |
Screen | WT1 | WT2 | WT3 | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 | AF100 | AF140 | AF180 |
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Mean Cell GFP Intensity (1e-4) | 5.5 | 6.7 | 6.7 | 6.9 | 6.1 | 4.4 | 3.8 | 4.1 | – | 3.5 | 3.8 | 3.6 | 8.0 | 8.0 | 7.4 | 5.2 | 6.3 | 7.3 | 9.0 | 8.5 | 7.9 |
Std Deviation (1e-4) | 1.2 | 1.4 | 1.5 | 1.7 | 1.6 | 0.9 | 0.9 | 0.9 | – | 0.8 | 1.0 | 0.8 | 1.3 | 1.3 | 1.4 | 1.1 | 1.3 | 1.4 | 1.7 | 1.8 | 1.5 |
Intensity Change (Log2) | – | – | – | 0.05 | -0.13 | -0.62 | -0.81 | -0.71 | – | -0.95 | -0.84 | -0.91 | 0.24 | 0.24 | 0.14 | -0.37 | -0.1 | 0.11 | 0.41 | 0.34 | 0.24 |
Localization | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 |
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Cortical Patches | 0 | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0.7 | 0.7 | 0 | 0 | 0 | 0 |
Bud | 0 | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Bud Neck | 0 | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Bud Site | 0 | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Cell Periphery | 0 | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 3.6 | 3.5 | 3.9 | 0 | 0 | 0 |
Cytoplasm | 3.9 | 13.1 | 17.2 | 18.6 | 17.7 | – | 18.3 | 12.9 | 13.7 | 4.9 | 4.6 | 7.2 | 6.2 | 7.5 | 7.5 |
Endoplasmic Reticulum | -1.5 | -13.6 | 0 | 0 | 0 | – | 0 | 0 | 0 | -2.6 | -1.9 | -3.7 | -6.1 | -8.9 | -8.8 |
Endosome | -1.7 | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | -3.7 | -3.7 | -3.5 | 0 | -0.4 | -0.6 |
Golgi | 0 | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Mitochondria | 0 | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Nucleus | 0 | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Nuclear Periphery | 0 | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Nucleolus | 0 | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Peroxisomes | 0 | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
SpindlePole | 0 | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Vacuole | -2.9 | -1.9 | -4.8 | -6.2 | -7.5 | – | -9.8 | -7.8 | -8.1 | -4.2 | -4.6 | -7.7 | -2.7 | -1.8 | -1.8 |
R1 | R2 | R3 | ||||||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | |
Concentration | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Actin | – | – | – | – | – | – | 0.0098 | 0.0004 | 0.0107 | 0.0003 | 0.0304 | 0.0003 | 0.0068 | 0.0013 | 0.0132 | 0.0283 | 0.0001 | 0.0001 |
Bud | – | – | – | – | – | – | 0.0004 | 0.0001 | 0.0001 | 0.0002 | 0.0002 | 0.0001 | 0.0002 | 0.0002 | 0.0011 | 0.0004 | 0.0001 | 0 |
Bud Neck | – | – | – | – | – | – | 0.0004 | 0.0002 | 0.0008 | 0.0004 | 0.0023 | 0.0009 | 0.0008 | 0.0002 | 0.0006 | 0.0134 | 0.0007 | 0.0005 |
Bud Periphery | – | – | – | – | – | – | 0.0008 | 0.0001 | 0.0002 | 0.0003 | 0.0003 | 0.0003 | 0.0002 | 0.0004 | 0.0037 | 0.0006 | 0.0001 | 0.0001 |
Bud Site | – | – | – | – | – | – | 0.0016 | 0.0011 | 0.0026 | 0.0006 | 0.0007 | 0.0002 | 0.003 | 0.0011 | 0.0043 | 0.0054 | 0.0001 | 0.0001 |
Cell Periphery | – | – | – | – | – | – | 0.0011 | 0.0009 | 0.0009 | 0.0009 | 0.0009 | 0.0006 | 0.0006 | 0.001 | 0.0015 | 0.0007 | 0.0004 | 0.0002 |
Cytoplasm | – | – | – | – | – | – | 0.1784 | 0.3999 | 0.3028 | 0.308 | 0.2953 | 0.3173 | 0.2083 | 0.382 | 0.2923 | 0.3024 | 0.2711 | 0.3279 |
Cytoplasmic Foci | – | – | – | – | – | – | 0.026 | 0.0107 | 0.0148 | 0.0125 | 0.0376 | 0.0109 | 0.0166 | 0.0196 | 0.0162 | 0.0133 | 0.0131 | 0.009 |
Eisosomes | – | – | – | – | – | – | 0.0002 | 0 | 0.0001 | 0 | 0.0001 | 0 | 0.0001 | 0.0002 | 0.0002 | 0.0004 | 0 | 0 |
Endoplasmic Reticulum | – | – | – | – | – | – | 0.4457 | 0.3503 | 0.4712 | 0.4282 | 0.3015 | 0.4393 | 0.524 | 0.3805 | 0.4317 | 0.456 | 0.4565 | 0.4979 |
Endosome | – | – | – | – | – | – | 0.0701 | 0.052 | 0.0572 | 0.0808 | 0.0691 | 0.0641 | 0.0535 | 0.0426 | 0.0589 | 0.0658 | 0.0687 | 0.0385 |
Golgi | – | – | – | – | – | – | 0.0109 | 0.0009 | 0.0019 | 0.0013 | 0.0185 | 0.0058 | 0.0051 | 0.0008 | 0.0029 | 0.0033 | 0.0009 | 0.0004 |
Lipid Particles | – | – | – | – | – | – | 0.0243 | 0.0058 | 0.0091 | 0.0057 | 0.0087 | 0.0046 | 0.0175 | 0.0048 | 0.009 | 0.0044 | 0.0068 | 0.0018 |
Mitochondria | – | – | – | – | – | – | 0.0172 | 0.0003 | 0.0015 | 0.0006 | 0.002 | 0.0015 | 0.0012 | 0.0035 | 0.0041 | 0.0012 | 0.0004 | 0.0003 |
None | – | – | – | – | – | – | 0.1095 | 0.1196 | 0.0479 | 0.0196 | 0.0575 | 0.0233 | 0.0581 | 0.0945 | 0.0685 | 0.0233 | 0.0685 | 0.0441 |
Nuclear Periphery | – | – | – | – | – | – | 0.033 | 0.0186 | 0.0216 | 0.0197 | 0.0463 | 0.0289 | 0.048 | 0.018 | 0.0293 | 0.0205 | 0.0337 | 0.0317 |
Nucleolus | – | – | – | – | – | – | 0.0009 | 0.0002 | 0.0006 | 0.0002 | 0.0002 | 0.0001 | 0.0009 | 0.0002 | 0.0002 | 0.0004 | 0.0001 | 0.0001 |
Nucleus | – | – | – | – | – | – | 0.0057 | 0.0047 | 0.0117 | 0.0041 | 0.0099 | 0.0131 | 0.0168 | 0.0084 | 0.0042 | 0.0032 | 0.004 | 0.0086 |
Peroxisomes | – | – | – | – | – | – | 0.0038 | 0.0001 | 0.0011 | 0.0001 | 0.0157 | 0.0001 | 0.0025 | 0.0033 | 0.003 | 0.0023 | 0.0001 | 0 |
Punctate Nuclear | – | – | – | – | – | – | 0.0075 | 0.0012 | 0.0017 | 0.0009 | 0.0012 | 0.0005 | 0.0073 | 0.0012 | 0.0017 | 0.0017 | 0.0006 | 0.0007 |
Vacuole | – | – | – | – | – | – | 0.049 | 0.0313 | 0.0377 | 0.1092 | 0.0969 | 0.0826 | 0.0256 | 0.0339 | 0.0477 | 0.0513 | 0.0658 | 0.0345 |
Vacuole Periphery | – | – | – | – | – | – | 0.0037 | 0.0016 | 0.0036 | 0.0062 | 0.0046 | 0.0056 | 0.0028 | 0.0021 | 0.0055 | 0.0018 | 0.0082 | 0.0034 |
R1 | R2 | |||||||||
---|---|---|---|---|---|---|---|---|---|---|
G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | |
Gene Expression | 21.7377 | 24.8732 | 19.0337 | 20.4277 | 21.9834 | 26.0708 | 19.2549 | 15.9132 | 15.9209 | 23.1137 |
Translational Efficiency | 0.4922 | 0.2627 | 0.4319 | 0.3895 | 0.3867 | 0.3669 | 0.3024 | 0.3303 | 0.3382 | 0.3094 |
Dataset | Hit |
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Protein Concentration | – |
Protein Localization | ✘ |
Gene Expression | ✘ |
Translational Efficiency | ✘ |
Temp | Actin Patch (Sac6-tdTomato) | Cortical Patch (Sla1-GFP) | Late Endosome (Snf7-GFP) | Vacuole (Vph1-GFP) |
---|---|---|---|---|
37℃ | ||||
RT |
Gene / Allele | Actin Patch (Sac6-tdTomato) | Cortical Patch (Sla1-GFP) | Late Endosome (Snf7-GFP) | Vacuole (Sac6-tdTomato) |
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Gene | Images |
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Gene | Images |
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