Standard name | |
---|---|
Human Ortholog | |
Description | Na+/H+ antiporter; involved in sodium and potassium efflux through the plasma membrane; required for alkali cation tolerance at acidic pH |
ensLOC | DeepLoc | |||||||||||||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Localization | WT1 | WT2 | WT3 | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 | WT1 | WT2 | WT3 | AF100 | AF140 | AF180 |
Cortical Patches | 101 | 110 | 209 | 192 | 287 | 225 | 204 | 167 | – | 219 | 204 | 214 | 65 | 68 | 37 | 30 | 40 | 45 | 31 | 49 | 41 | 28 | 43 | 40 |
Bud | 17 | 39 | 30 | 39 | 50 | 46 | 45 | 75 | – | 92 | 76 | 90 | 12 | 12 | 10 | 3 | 6 | 11 | 1 | 2 | 1 | 0 | 2 | 1 |
Bud Neck | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 8 | 12 | 4 | 4 | 5 | 15 |
Bud Site | 1 | 1 | 1 | 0 | 1 | 0 | 0 | 2 | – | 4 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | – | – | – | – | – | – |
Cell Periphery | 197 | 186 | 285 | 275 | 306 | 285 | 296 | 157 | – | 225 | 231 | 250 | 233 | 262 | 247 | 33 | 32 | 91 | 320 | 261 | 428 | 260 | 442 | 507 |
Cytoplasm | 72 | 79 | 42 | 72 | 52 | 67 | 96 | 151 | – | 180 | 177 | 237 | 63 | 84 | 86 | 139 | 101 | 80 | 5 | 8 | 3 | 0 | 4 | 6 |
Endoplasmic Reticulum | 4 | 10 | 6 | 16 | 29 | 18 | 32 | 26 | – | 39 | 19 | 30 | 5 | 9 | 7 | 8 | 10 | 16 | 10 | 12 | 9 | 4 | 9 | 13 |
Endosome | 1 | 0 | 0 | 2 | 2 | 3 | 3 | 11 | – | 0 | 2 | 3 | 1 | 1 | 1 | 0 | 0 | 0 | 1 | 0 | 1 | 0 | 0 | 3 |
Golgi | 1 | 1 | 0 | 0 | 2 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 0 | 1 | 1 | 1 |
Mitochondria | 33 | 43 | 115 | 167 | 191 | 218 | 283 | 347 | – | 304 | 262 | 337 | 2 | 1 | 0 | 25 | 22 | 22 | 6 | 10 | 11 | 11 | 9 | 7 |
Nucleus | 1 | 0 | 2 | 1 | 0 | 1 | 1 | 2 | – | 2 | 2 | 2 | 1 | 1 | 2 | 2 | 1 | 2 | 0 | 0 | 0 | 0 | 0 | 0 |
Nuclear Periphery | 0 | 0 | 2 | 0 | 0 | 0 | 4 | 16 | – | 0 | 1 | 5 | 0 | 0 | 0 | 2 | 1 | 0 | 0 | 0 | 1 | 0 | 0 | 0 |
Nucleolus | 0 | 0 | 0 | 0 | 1 | 0 | 0 | 3 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Peroxisomes | 8 | 0 | 42 | 43 | 88 | 43 | 40 | 37 | – | 38 | 12 | 30 | 0 | 0 | 0 | 0 | 0 | 1 | 0 | 0 | 0 | 0 | 0 | 0 |
SpindlePole | 15 | 1 | 13 | 8 | 6 | 3 | 9 | 10 | – | 10 | 4 | 5 | 5 | 4 | 5 | 4 | 3 | 5 | 0 | 0 | 0 | 0 | 0 | 0 |
Vac/Vac Membrane | 0 | 0 | 0 | 0 | 2 | 1 | 1 | 6 | – | 3 | 1 | 9 | 0 | 1 | 0 | 0 | 2 | 0 | 3 | 4 | 0 | 0 | 0 | 4 |
Unique Cell Count | 318 | 313 | 429 | 463 | 561 | 478 | 608 | 707 | 709 | 614 | 801 | 302 | 352 | 329 | 209 | 187 | 208 | 391 | 366 | 507 | 316 | 522 | 608 | |
Labelled Cell Count | 451 | 470 | 747 | 815 | 1017 | 910 | 1014 | 1010 | 1116 | 992 | 1212 | 387 | 443 | 395 | 246 | 218 | 273 | 391 | 366 | 507 | 316 | 522 | 608 |
Screen | WT1 | WT2 | WT3 | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 | AF100 | AF140 | AF180 |
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Mean Cell GFP Intensity (1e-4) | 7.5 | 7.4 | 6.8 | 7.3 | 7.8 | 7.0 | 6.9 | 6.8 | – | 6.2 | 6.5 | 6.5 | 8.3 | 8.1 | 8.6 | 5.6 | 6.6 | 8.4 | 8.5 | 8.9 | 9.2 |
Std Deviation (1e-4) | 0.9 | 1.3 | 1.2 | 1.3 | 1.4 | 1.3 | 1.3 | 1.2 | – | 1.2 | 1.2 | 1.2 | 1.0 | 1.0 | 1.0 | 1.3 | 1.4 | 2.2 | 1.2 | 1.5 | 1.5 |
Intensity Change (Log2) | – | – | – | 0.11 | 0.19 | 0.05 | 0.01 | -0.0 | – | -0.14 | -0.07 | -0.07 | 0.29 | 0.25 | 0.34 | -0.29 | -0.04 | 0.31 | 0.33 | 0.39 | 0.44 |
Localization | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 |
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Cortical Patches | -2.2 | 0.8 | -0.5 | -4.9 | -8.7 | – | -6.0 | -5.0 | -7.7 | -7.5 | -8.5 | -10.9 | -8.4 | -6.4 | -6.5 |
Bud | 0.8 | 1.1 | 1.4 | 0.3 | 2.0 | – | 3.2 | 2.8 | 2.4 | -1.7 | -2.2 | -2.4 | -3.0 | -1.8 | -0.8 |
Bud Neck | 0 | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Bud Site | 0 | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Cell Periphery | -2.2 | -3.8 | -2.1 | -5.7 | -14.8 | – | -11.4 | -9.2 | -11.9 | 3.1 | 2.4 | 2.6 | -12.0 | -11.3 | -5.5 |
Cytoplasm | 2.6 | -0.3 | 2.0 | 2.8 | 5.0 | – | 6.4 | 7.4 | 7.9 | 4.2 | 5.3 | 6.0 | 14.9 | 12.0 | 8.6 |
Endoplasmic Reticulum | 2.0 | 3.2 | 2.2 | 3.3 | 2.3 | – | 3.4 | 1.8 | 2.3 | 0 | 1.2 | 0.8 | 0 | 0 | 4.1 |
Endosome | 0 | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Golgi | 0 | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Mitochondria | 3.0 | 2.4 | 5.9 | 6.4 | 7.4 | – | 5.4 | 5.2 | 5.3 | -9.5 | -10.4 | -10.2 | -4.3 | -4.1 | -4.7 |
Nucleus | 0 | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Nuclear Periphery | 0 | 0 | 0 | 0 | 2.4 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Nucleolus | 0 | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Peroxisomes | -0.3 | 2.7 | -0.4 | -1.9 | -2.9 | – | -2.8 | -5.6 | -4.3 | -5.6 | -6.0 | -5.8 | -4.7 | -4.4 | -4.4 |
SpindlePole | -1.3 | -2.2 | -2.7 | -1.7 | -1.9 | – | -1.9 | -3.0 | -3.3 | -1.2 | -1.8 | -1.4 | -0.8 | 0 | -0.4 |
Vacuole | 0 | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
R1 | R2 | R3 | ||||||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | |
Concentration | 2.6236 | 4.6849 | 3.7605 | 3.1304 | 3.3073 | 3.7779 | 2.3659 | 4.9776 | 3.6993 | 3.3693 | 3.1576 | 3.7177 | 2.6599 | 4.4885 | 3.224 | 3.6672 | 2.8366 | 3.2272 |
Actin | 0.0305 | 0.0144 | 0.0113 | 0.0092 | 0.0632 | 0.0131 | 0.1049 | 0.011 | 0.0198 | 0.0248 | 0.0133 | 0.0144 | 0.0582 | 0.0033 | 0.0375 | 0.0234 | 0.0085 | 0.036 |
Bud | 0.0009 | 0.0005 | 0.0007 | 0.0005 | 0.0019 | 0.0006 | 0.0016 | 0.0087 | 0.0062 | 0.0019 | 0.0006 | 0.0055 | 0.0002 | 0.0002 | 0.0009 | 0.0014 | 0.0005 | 0.0005 |
Bud Neck | 0.0057 | 0.0061 | 0.0293 | 0.0262 | 0.0307 | 0.0199 | 0.0126 | 0.0037 | 0.0097 | 0.0443 | 0.0043 | 0.0176 | 0.011 | 0.0065 | 0.0295 | 0.0115 | 0.0035 | 0.0189 |
Bud Periphery | 0.0061 | 0.0032 | 0.0049 | 0.0063 | 0.0137 | 0.0086 | 0.0107 | 0.0037 | 0.0097 | 0.0171 | 0.0101 | 0.018 | 0.0023 | 0.0015 | 0.0044 | 0.0102 | 0.0024 | 0.008 |
Bud Site | 0.0165 | 0.067 | 0.0457 | 0.011 | 0.0402 | 0.0028 | 0.0495 | 0.0784 | 0.052 | 0.0163 | 0.003 | 0.0046 | 0.0173 | 0.1503 | 0.0628 | 0.0145 | 0.0008 | 0.0043 |
Cell Periphery | 0.5779 | 0.5862 | 0.653 | 0.6782 | 0.5954 | 0.6824 | 0.4628 | 0.7061 | 0.7089 | 0.6799 | 0.611 | 0.7769 | 0.3853 | 0.5854 | 0.6008 | 0.6855 | 0.6221 | 0.6427 |
Cytoplasm | 0.1981 | 0.2651 | 0.201 | 0.1489 | 0.1109 | 0.2023 | 0.1827 | 0.1397 | 0.1292 | 0.1255 | 0.1321 | 0.105 | 0.3043 | 0.248 | 0.2252 | 0.1528 | 0.0361 | 0.254 |
Cytoplasmic Foci | 0.012 | 0.0019 | 0.0021 | 0.0092 | 0.0229 | 0.0025 | 0.0061 | 0.0038 | 0.0107 | 0.0022 | 0.0097 | 0.0055 | 0.0085 | 0.0003 | 0.0028 | 0.0074 | 0.0018 | 0.0027 |
Eisosomes | 0.0371 | 0.0236 | 0.0143 | 0.0219 | 0.0207 | 0.0209 | 0.0727 | 0.0195 | 0.0152 | 0.0252 | 0.0112 | 0.0128 | 0.1152 | 0.0012 | 0.0121 | 0.0064 | 0.0011 | 0.0063 |
Endoplasmic Reticulum | 0.0134 | 0.0113 | 0.011 | 0.007 | 0.0097 | 0.0132 | 0.0089 | 0.0016 | 0.0057 | 0.0383 | 0.0026 | 0.0051 | 0.0159 | 0.0007 | 0.0052 | 0.0118 | 0.002 | 0.0034 |
Endosome | 0.0135 | 0.001 | 0.0017 | 0.0076 | 0.0165 | 0.0006 | 0.0031 | 0.0032 | 0.0037 | 0.0021 | 0.0019 | 0.0053 | 0.002 | 0 | 0.0012 | 0.02 | 0.0054 | 0.0008 |
Golgi | 0.008 | 0.0022 | 0.002 | 0.0071 | 0.013 | 0.0009 | 0.008 | 0.0059 | 0.0041 | 0.0019 | 0.003 | 0.0065 | 0.0042 | 0.0001 | 0.0033 | 0.0146 | 0.0095 | 0.0031 |
Lipid Particles | 0.0092 | 0.0016 | 0.0025 | 0.0268 | 0.0192 | 0.0022 | 0.0054 | 0.0005 | 0.004 | 0.0045 | 0.0439 | 0.0073 | 0.0172 | 0.0002 | 0.0019 | 0.006 | 0.0008 | 0.0092 |
Mitochondria | 0.0108 | 0.0002 | 0.0004 | 0.004 | 0.0079 | 0.0003 | 0.0029 | 0.0016 | 0.0095 | 0.0006 | 0.0395 | 0.0017 | 0.0001 | 0 | 0.0004 | 0.0224 | 0.3016 | 0.0007 |
None | 0.0377 | 0.0123 | 0.0117 | 0.0214 | 0.0057 | 0.0149 | 0.0596 | 0.0089 | 0.0051 | 0.0085 | 0.0523 | 0.0085 | 0.0529 | 0.0016 | 0.0036 | 0.0032 | 0.0003 | 0.0029 |
Nuclear Periphery | 0.0058 | 0.0005 | 0.0013 | 0.0003 | 0.0032 | 0.0031 | 0.001 | 0.0002 | 0.0003 | 0.0012 | 0.0003 | 0.0003 | 0.0009 | 0.0001 | 0.0003 | 0.001 | 0.0002 | 0.0003 |
Nucleolus | 0.0008 | 0.0002 | 0.0007 | 0.0002 | 0.001 | 0.0033 | 0.0003 | 0.0002 | 0.0003 | 0.0003 | 0.0002 | 0.0001 | 0.0001 | 0 | 0.0003 | 0.0003 | 0 | 0 |
Nucleus | 0.0044 | 0.0003 | 0.0011 | 0.0002 | 0.0013 | 0.0035 | 0.0006 | 0.0008 | 0.0004 | 0.0005 | 0.0002 | 0.0003 | 0.0002 | 0.0001 | 0.0003 | 0.0006 | 0.0001 | 0.0001 |
Peroxisomes | 0.0026 | 0.0004 | 0.0004 | 0.0117 | 0.0118 | 0.0002 | 0.0027 | 0.0005 | 0.0026 | 0.0005 | 0.0585 | 0.0017 | 0.0002 | 0 | 0.003 | 0.001 | 0.0023 | 0.0039 |
Punctate Nuclear | 0.0046 | 0.0002 | 0.003 | 0.0005 | 0.007 | 0.0011 | 0.0005 | 0.0001 | 0.0003 | 0.0003 | 0.001 | 0.0003 | 0.0004 | 0 | 0.0001 | 0.0002 | 0 | 0.0003 |
Vacuole | 0.004 | 0.0016 | 0.0017 | 0.0016 | 0.0035 | 0.0033 | 0.0033 | 0.0019 | 0.0023 | 0.0038 | 0.001 | 0.0024 | 0.0035 | 0.0007 | 0.0043 | 0.0051 | 0.0006 | 0.0015 |
Vacuole Periphery | 0.0005 | 0.0001 | 0.0001 | 0.0002 | 0.0005 | 0.0001 | 0.0002 | 0.0001 | 0.0001 | 0.0003 | 0.0002 | 0.0001 | 0 | 0 | 0.0001 | 0.0009 | 0.0005 | 0.0001 |
R1 | R2 | |||||||||
---|---|---|---|---|---|---|---|---|---|---|
G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | |
Gene Expression | 27.7903 | 34.3464 | 41.0754 | 35.2053 | 32.1622 | 32.0623 | 33.8004 | 36.4098 | 43.5348 | 38.1762 |
Translational Efficiency | 0.8353 | 0.6741 | 0.5315 | 0.6069 | 0.5078 | 0.6071 | 0.6044 | 0.4825 | 0.4192 | 0.5002 |
Dataset | Hit |
---|---|
Protein Concentration | ✘ |
Protein Localization | ✘ |
Gene Expression | ✘ |
Translational Efficiency | ✘ |
R1 | R2 | R1 & R2 | |||||||||
---|---|---|---|---|---|---|---|---|---|---|---|
WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 |
1170 | 1653 | 411 | 350 | 1234 | 1976 | 1994 | 1817 | 2404 | 3629 | 2405 | 2167 |
R1 | R2 | R1 & R2 | ||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|
WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 | |
Mean | 907.34 | 864.27 | 1101.13 | 1109.21 | 818.46 | 882.35 | 946.53 | 1003.73 | 861.72 | 874.11 | 972.95 | 1020.77 |
Standard Deviation | 110.46 | 147.49 | 126.00 | 157.20 | 111.44 | 109.47 | 121.61 | 125.17 | 119.53 | 128.51 | 135.51 | 136.51 |
Intensity Change Log 2 | — | -0.070161 | 0.279270 | 0.289817 | — | 0.108439 | 0.209736 | 0.294387 | — | 0.017300 | 0.246711 | 0.291987 |
R1 | R2 | R1 & R2 | ||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|
WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 | |
Actin | 0.039871 | 0.032390 | 0.093888 | 0.082369 | 0.007014 | 0.011272 | 0.036075 | 0.096921 | 0.023005 | 0.020891 | 0.045955 | 0.094570 |
Bud Neck | 0.050748 | 0.028610 | 0.028614 | 0.036876 | 0.012453 | 0.021490 | 0.034928 | 0.035969 | 0.031090 | 0.024733 | 0.033849 | 0.036116 |
Bud Site | 0.073459 | 0.068603 | 0.168398* | 0.145527* | 0.022054 | 0.021898 | 0.061142 | 0.132728* | 0.047072 | 0.043172 | 0.079472 | 0.134795* |
Cell Periphery | 0.168742* | 0.449062* | 0.337874* | 0.501871* | 0.614752* | 0.596499* | 0.564377* | 0.437897* | 0.397684* | 0.529342* | 0.525669* | 0.448229* |
Cytoplasm | 0.338025* | 0.263049* | 0.146745* | 0.054108 | 0.210800* | 0.238332* | 0.176494* | 0.174782* | 0.272719* | 0.249591* | 0.171410* | 0.155292* |
Cytoplasmic Foci | 0.043554 | 0.021739 | 0.014535 | 0.001044 | 0.007217 | 0.005234 | 0.002769 | 0.002697 | 0.024902 | 0.012752 | 0.004779 | 0.002430 |
Eisosomes | 0.102229* | 0.035576 | 0.046101 | 0.089720 | 0.025609 | 0.053049 | 0.068258 | 0.073484 | 0.062899 | 0.045090 | 0.064472 | 0.076106 |
Endoplasmic Reticulum | 0.011248 | 0.012878 | 0.079148 | 0.024655 | 0.018090 | 0.005412 | 0.013865 | 0.013254 | 0.014760 | 0.008813 | 0.025022 | 0.015095 |
Endosome | 0.008253 | 0.006356 | 0.003069 | 0.001155 | 0.002434 | 0.001548 | 0.001095 | 0.001028 | 0.005266 | 0.003738 | 0.001433 | 0.001049 |
Golgi | 0.009767 | 0.013579 | 0.008524 | 0.001200 | 0.002323 | 0.003134 | 0.001478 | 0.001662 | 0.005946 | 0.007892 | 0.002682 | 0.001588 |
Lipid Particles | 0.016426 | 0.013604 | 0.013447 | 0.006572 | 0.005308 | 0.003119 | 0.004157 | 0.003039 | 0.010719 | 0.007895 | 0.005744 | 0.003610 |
Mitochondria | 0.003716 | 0.009309 | 0.000519 | 0.000345 | 0.004923 | 0.004348 | 0.003156 | 0.002030 | 0.004336 | 0.006608 | 0.002706 | 0.001758 |
Mitotic Spindle | 0.002740 | 0.000229 | 0.000367 | 0.000337 | 0.000436 | 0.000183 | 0.000366 | 0.000308 | 0.001557 | 0.000204 | 0.000366 | 0.000313 |
None | 0.001095 | 0.000297 | 0.000102 | 0.000144 | 0.000730 | 0.000182 | 0.000310 | 0.000166 | 0.000908 | 0.000235 | 0.000274 | 0.000162 |
Nuclear Periphery | 0.000255 | 0.000149 | 0.000147 | 0.000186 | 0.000215 | 0.000194 | 0.000305 | 0.000112 | 0.000235 | 0.000174 | 0.000278 | 0.000124 |
Nuclear Periphery Foci | 0.001429 | 0.000372 | 0.004072 | 0.001520 | 0.000233 | 0.000173 | 0.000284 | 0.000382 | 0.000815 | 0.000264 | 0.000931 | 0.000566 |
Nucleolus | 0.000238 | 0.000315 | 0.000026 | 0.000010 | 0.000280 | 0.000046 | 0.000057 | 0.000028 | 0.000260 | 0.000169 | 0.000052 | 0.000025 |
Nucleus | 0.031598 | 0.002168 | 0.011644 | 0.005405 | 0.007753 | 0.002559 | 0.004471 | 0.006944 | 0.019359 | 0.002381 | 0.005697 | 0.006695 |
Peroxisomes | 0.006015 | 0.036227 | 0.002357 | 0.000988 | 0.050064 | 0.027925 | 0.020460 | 0.004140 | 0.028626 | 0.031706 | 0.017366 | 0.003631 |
Vacuole | 0.087464 | 0.004614 | 0.040222 | 0.045528 | 0.006422 | 0.003156 | 0.005795 | 0.012072 | 0.045864 | 0.003820 | 0.011678 | 0.017475 |
Vacuole Periphery | 0.003128 | 0.000874 | 0.000200 | 0.000441 | 0.000891 | 0.000248 | 0.000156 | 0.000356 | 0.001980 | 0.000533 | 0.000163 | 0.000370 |
R1 | R2 | R1 & R2 | |||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
UBP2_WT | UBP14_WT | UBP2UBP14_WT | UBP2UBP14_UBP2 | UBP2UBP14_UBP14 | UBP2_WT | UBP14_WT | UBP2UBP14_WT | UBP2UBP14_UBP2 | UBP2UBP14_UBP14 | UBP2_WT | UBP14_WT | UBP2UBP14_WT | UBP2UBP14_UBP2 | UBP2UBP14_UBP14 | |
Actin | 2.30 | -7.91 | -5.57 | -6.68 | 1.21 | -3.99 | -14.80 | -24.47* | -22.98* | -14.94 | 1.20 | -9.88 | -20.45 | -21.67 | -12.81 |
Bud Neck | 7.23 | 6.58 | 3.18 | -2.18 | -2.05 | -5.12 | -10.49 | -11.84 | -7.08 | -0.43 | 3.48 | -1.42 | -2.54 | -6.54 | -1.07 |
Bud Site | 0.92 | -9.66 | -6.73 | -7.45 | 1.81 | 0.06 | -10.62 | -21.92 | -22.99 | -13.14 | 1.33 | -8.42 | -18.32 | -20.41 | -10.78 |
Cell Periphery | -24.84* | -11.23 | -19.16 | -2.66 | -7.77 | 1.54 | 4.31 | 15.19 | 15.56 | 12.50 | -14.13 | -13.02 | -5.05 | 9.37 | 8.42 |
Cytoplasm | 8.11 | 17.86 | 34.61 | 29.15 | 10.30 | -3.38 | 4.50 | 4.67 | 9.16 | 0.24 | 3.64 | 16.20 | 18.71 | 16.97 | 2.89 |
Cytoplasmic Foci | 6.85 | 9.19 | 15.15 | 12.06 | 8.76 | 2.25 | 5.98 | 5.52 | 3.59 | -0.16 | 7.40 | 13.50 | 15.04 | 11.38 | 4.78 |
Eisosomes | 14.66 | 10.57 | 1.64 | -8.65 | -6.40 | -11.15 | -15.15 | -16.46 | -6.34 | -1.46 | 6.52 | -0.69 | -4.16 | -11.55 | -3.68 |
Endoplasmic Reticulum | -0.97 | -11.38 | -3.87 | -3.33 | 8.00 | 6.12 | 1.83 | 2.15 | -6.90 | 0.43 | 4.46 | -5.56 | -0.25 | -5.21 | 5.59 |
Endosome | 1.73 | 5.28 | 6.88 | 7.02 | 3.61 | 2.09 | 3.50 | 3.28 | 1.79 | 0.27 | 2.65 | 7.60 | 7.94 | 7.67 | 1.83 |
Golgi | -2.78 | 0.98 | 8.39 | 11.36 | 9.36 | -1.95 | 3.20 | 2.12 | 3.28 | -0.58 | -2.82 | 6.18 | 8.19 | 11.16 | 3.66 |
Lipid Particles | 1.43 | 1.51 | 3.68 | 2.71 | 2.70 | 3.29 | 1.62 | 2.90 | 0.09 | 1.68 | 2.77 | 5.47 | 7.18 | 5.05 | 3.06 |
Mitochondria | -4.16 | 2.98 | 3.19 | 10.40 | 0.86 | 0.74 | 2.51 | 4.22 | 4.28 | 2.69 | -2.99 | 2.45 | 3.98 | 9.30 | 2.70 |
Mitotic Spindle | 3.17 | 2.97 | 3.01 | -1.24 | 0.42 | 0.77 | 0.22 | 0.38 | -1.38 | 0.60 | 3.21 | 2.82 | 2.90 | -1.42 | 0.67 |
None | 0.93 | 1.16 | 1.11 | 1.54 | -0.46 | 1.68 | 1.29 | 1.74 | 0.52 | 3.99 | 1.50 | 1.41 | 1.67 | 2.10 | 3.35 |
Nuclear Periphery | 1.84 | 1.87 | 0.91 | -1.02 | -1.05 | 1.73 | -4.95 | 8.91 | 9.54 | 12.14 | 2.13 | -1.37 | 3.73 | 4.78 | 10.27 |
Nuclear Periphery Foci | 4.94 | -5.66 | -0.29 | -2.54 | 3.93 | 1.53 | -1.25 | -3.55 | -8.56 | -3.82 | 5.29 | -0.89 | 2.14 | -3.18 | 3.15 |
Nucleolus | -0.29 | 1.48 | 1.58 | 1.50 | 6.41 | 1.63 | 1.56 | 1.75 | 4.65 | 11.71 | 0.69 | 2.05 | 2.31 | 1.54 | 12.52 |
Nucleus | 17.94 | 9.41 | 12.10 | -3.37 | 2.71 | 3.17 | 2.03 | 0.49 | -10.16 | -5.68 | 14.26 | 11.25 | 9.98 | -10.74 | -2.55 |
Peroxisomes | -13.83 | 7.06 | 9.41 | 16.43 | 4.44 | 6.60 | 9.13 | 15.47 | 14.49 | 11.60 | -1.50 | 5.75 | 15.39 | 20.91 | 11.67 |
Vacuole | 18.08 | 8.60 | 6.07 | -8.89 | -1.39 | 4.47 | 0.86 | -5.78 | -11.52 | -8.16 | 17.46 | 13.86 | 10.71 | -13.22 | -5.27 |
Vacuole Periphery | 2.57 | 3.38 | 2.77 | 0.95 | -0.65 | 2.38 | 2.74 | 1.83 | -0.89 | -1.69 | 3.23 | 4.10 | 3.50 | 1.20 | -1.76 |
Temp | Actin Patch (Sac6-tdTomato) | Cortical Patch (Sla1-GFP) | Late Endosome (Snf7-GFP) | Vacuole (Vph1-GFP) |
---|---|---|---|---|
37℃ | ||||
RT |
Gene / Allele | Actin Patch (Sac6-tdTomato) | Cortical Patch (Sla1-GFP) | Late Endosome (Snf7-GFP) | Vacuole (Sac6-tdTomato) |
---|
Gene | Images |
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Gene | Images |
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