Standard name
Human Ortholog
Description Diphthamide synthetase; catalyzes the last amidation step of diphthamide biosynthesis using ammonium and ATP; evolutionarily conserved in eukaryotes; dph6 mutants exhibit diphthine accumulation and resistance to sordarin, which is indicative of defects in diphthamide formation on EF2; green fluorescent protein (GFP)-tagged protein localizes to the cytoplasm; DPH6/YLR143W is not an essential gene
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Micrographs

ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 – – – 0 0 0 0 0 0
Bud 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 – – – 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 – – – 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 – – – – – – – – –
Cell Periphery 0 0 0.06 0 0 0 0 – 0 0 0 0 0 0 0 – – – 0 0 0 0 0 0
Cytoplasm 1.0 0.99 0.97 0.97 0.97 0.95 0.92 – 0.79 0.83 0.73 0.74 0.99 0.98 0.98 – – – 0.97 0.95 0.95 0.93 0.91 0.91
Endoplasmic Reticulum 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 – – – 0 0 0 0 0 0
Endosome 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 – – – 0 0 0 0 0 0
Golgi 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 – – – 0 0 0 0 0 0
Mitochondria 0 0 0 0 0 0.05 0.09 – 0.26 0.14 0.37 0.26 0 0 0 – – – 0 0 0 0 0 0
Nucleus 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 – – – 0 0 0 0 0 0
Nuclear Periphery 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 – – – 0 0 0 0 0 0
Nucleolus 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 – – – 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 – – – 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 – – – 0 0 0 0 0 0
Vac/Vac Membrane 0 0 0 0 0 0 0 – 0.05 0 0 0.07 0 0 0 – – – 0 0 0 0 0 0
ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 – – – 0 1 0 0 1 1
Bud 1 0 0 0 0 0 1 – 1 2 2 1 0 0 0 – – – 0 0 0 1 2 4
Bud Neck 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 – – – 0 0 0 0 0 1
Bud Site 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 – – – – – – – – –
Cell Periphery 0 2 13 0 3 1 4 – 1 1 6 4 1 1 0 – – – 0 0 0 0 0 0
Cytoplasm 214 331 197 146 226 190 196 – 186 198 202 209 302 308 285 – – – 207 317 192 184 249 315
Endoplasmic Reticulum 2 3 2 1 0 2 1 – 1 2 3 2 1 2 1 – – – 1 1 1 0 1 3
Endosome 0 0 0 2 2 0 1 – 1 1 0 3 1 0 3 – – – 2 3 0 0 4 4
Golgi 0 0 0 0 0 0 0 – 0 0 0 0 1 0 0 – – – 1 3 0 0 0 1
Mitochondria 0 0 0 1 3 11 20 – 61 34 102 74 0 1 0 – – – 0 2 1 1 3 4
Nucleus 0 2 2 3 1 0 3 – 1 4 4 8 1 1 0 – – – 0 0 0 0 0 1
Nuclear Periphery 0 1 1 0 0 0 0 – 0 0 1 0 0 1 0 – – – 0 0 0 0 0 0
Nucleolus 0 0 0 0 0 0 0 – 0 0 0 1 0 0 0 – – – 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 – – – 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 – 1 0 0 0 0 0 0 – – – 0 1 0 1 2 1
Vac/Vac Membrane 0 2 4 4 8 3 6 – 11 7 8 20 2 2 6 – – – 0 1 0 2 1 1
Unique Cell Count 215 336 204 151 234 201 214 235 238 276 282 306 313 292 213 335 203 198 273 348
Labelled Cell Count 217 341 219 157 243 207 232 264 249 328 322 309 316 295 213 335 203 198 273 348


Cytoplasm

(Huh et al. Nature 2003 425:686)
Screen WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 AF100 AF140 AF180
Mean Cell GFP Intensity (1e-4) 6.5 7.8 7.5 7.3 7.7 6.3 6.3 – 5.6 5.5 5.4 5.4 8.0 8.1 8.0 – – – 7.5 7.7 7.7
Std Deviation (1e-4) 0.7 1.0 1.3 1.2 2.2 2.0 1.6 – 1.7 1.3 2.0 2.2 1.1 1.3 1.8 – – – 1.2 2.1 1.3
Intensity Change (Log2) – – – -0.03 0.03 -0.24 -0.24 – -0.43 -0.44 -0.47 -0.48 0.09 0.1 0.08 – – – -0.01 0.04 0.03


Localization RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3
Cortical Patches 0 0 0 0 – 0 0 0 0 0 0 0 – – –
Bud 0 0 0 0 – 0 0 0 0 0 0 0 – – –
Bud Neck 0 0 0 0 – 0 0 0 0 0 0 0 – – –
Bud Site 0 0 0 0 – 0 0 0 0 0 0 0 – – –
Cell Periphery -3.2 -2.8 -3.2 -2.3 – -3.5 -3.6 -2.3 -2.9 -4.1 -4.1 -4.4 – – –
Cytoplasm 0.1 0 -1.0 -2.1 – -5.5 -4.5 -6.8 -6.6 1.6 1.4 0.7 – – –
Endoplasmic Reticulum 0 0 0 0 – 0 0 0 0 0 0 0 – – –
Endosome 0 0 0 0 – 0 0 0 0 0 0 0 – – –
Golgi 0 0 0 0 – 0 0 0 0 0 0 0 – – –
Mitochondria 0 0 3.4 4.5 – 7.8 5.6 9.8 7.9 0 0 0 – – –
Nucleus 0 0 0 0 – 0 0 0 0 0 0 0 – – –
Nuclear Periphery 0 0 0 0 – 0 0 0 0 0 0 0 – – –
Nucleolus 0 0 0 0 – 0 0 0 0 0 0 0 – – –
Peroxisomes 0 0 0 0 – 0 0 0 0 0 0 0 – – –
SpindlePole 0 0 0 0 – 0 0 0 0 0 0 0 – – –
Vacuole 0 0.9 0 0 – 1.6 0.7 0.7 2.6 0 0 0 – – –

Images

Circle plot not available

Protein Concentration and Protein Localization Data

R1 R2 R3
G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase
Concentration 1.4853 2.6649 2.1004 1.3699 1.5773 2.327 2.5182 3.5932 3.1178 2.6158 2.8688 3.1902 3.6203 4.5213 3.9783 3.7406 3.3493 3.7394
Actin 0.0322 0.0008 0.0229 0.0317 0.0034 0.0135 0.0253 0.0042 0.0018 0.0007 0.0012 0.0009 0.0504 0.0007 0.0138 0.0507 0.0186 0.0077
Bud 0.0023 0.0065 0.0033 0.0157 0.0019 0.0003 0.0028 0.0051 0.0109 0.0002 0.001 0.0118 0.001 0.0001 0.0018 0.0225 0.0007 0.0007
Bud Neck 0.0009 0.0004 0.0006 0.0025 0.0009 0.0009 0.0056 0.0003 0.0008 0.0004 0.0004 0.0018 0.0019 0.0001 0.009 0.0014 0.001 0.004
Bud Periphery 0.0015 0.0034 0.0024 0.0093 0.0008 0.0002 0.003 0.0018 0.0048 0.0001 0.0011 0.0057 0.0012 0 0.0026 0.0121 0.0006 0.0007
Bud Site 0.007 0.0071 0.0057 0.0116 0.0006 0.0002 0.0113 0.0096 0.0142 0.0003 0.0035 0.0009 0.0029 0.0008 0.004 0.0047 0.0022 0.0014
Cell Periphery 0.0007 0.0002 0.0002 0.0006 0.0001 0 0.0008 0.0002 0.0003 0 0.0002 0.0001 0.0002 0 0.0001 0.0004 0.0001 0.0001
Cytoplasm 0.7025 0.8843 0.7991 0.498 0.7531 0.8881 0.6832 0.8504 0.8556 0.7579 0.6657 0.8739 0.7708 0.9421 0.8693 0.7985 0.7957 0.9121
Cytoplasmic Foci 0.0266 0.0103 0.0141 0.0451 0.0161 0.0099 0.025 0.0129 0.0048 0.0071 0.0203 0.0068 0.0261 0.0089 0.0147 0.0177 0.0569 0.0105
Eisosomes 0.0004 0 0.0008 0.0003 0.0002 0 0.0002 0 0 0 0 0 0.0003 0 0 0.0002 0.0001 0
Endoplasmic Reticulum 0.0082 0.0041 0.0029 0.0022 0.0031 0.0024 0.0079 0.0022 0.0022 0.0029 0.0038 0.0019 0.007 0.0012 0.0043 0.003 0.0033 0.0029
Endosome 0.0407 0.0112 0.03 0.0931 0.067 0.0155 0.0306 0.0054 0.0047 0.0047 0.1239 0.002 0.0228 0.0028 0.0146 0.007 0.0389 0.008
Golgi 0.0096 0.0009 0.0127 0.0964 0.0134 0.0039 0.0075 0.0012 0.0041 0.0013 0.0123 0.0001 0.0084 0.0001 0.004 0.0032 0.0194 0.0047
Lipid Particles 0.0089 0.0003 0.0059 0.0452 0.0035 0.0131 0.0073 0.0021 0.0003 0.0018 0.0034 0 0.0061 0.0001 0.0032 0.0011 0.0096 0.0045
Mitochondria 0.0082 0.0008 0.0111 0.0456 0.0403 0.0011 0.0162 0.0013 0.0021 0.0396 0.0579 0.0002 0.0066 0.0001 0.0022 0.0019 0.0048 0.0034
None 0.1144 0.0628 0.0729 0.0305 0.0315 0.0361 0.1254 0.0932 0.0842 0.1686 0.0725 0.0814 0.068 0.0386 0.0361 0.0448 0.0212 0.0219
Nuclear Periphery 0.0079 0.0014 0.0019 0.0016 0.0148 0.0041 0.0111 0.001 0.0016 0.0008 0.0016 0.001 0.0073 0.0006 0.0028 0.0096 0.0023 0.002
Nucleolus 0.003 0.0001 0.0006 0.001 0.0039 0.0001 0.001 0.0001 0.0002 0.0001 0.0002 0.0002 0.0003 0.0001 0.0001 0.0006 0.0002 0.0001
Nucleus 0.0063 0.0024 0.0034 0.0043 0.0181 0.0038 0.0089 0.0037 0.0043 0.0017 0.0015 0.0079 0.0039 0.0018 0.0036 0.0089 0.0051 0.0069
Peroxisomes 0.0052 0.0005 0.0029 0.0484 0.0013 0.001 0.0134 0.0016 0.0004 0.0102 0.0052 0.0001 0.0085 0.0001 0.0095 0.0029 0.0042 0.005
Punctate Nuclear 0.0085 0.0007 0.0028 0.0022 0.0143 0.0033 0.0057 0.0025 0.0014 0.0004 0.0005 0.0021 0.0025 0.0009 0.0021 0.007 0.0122 0.0023
Vacuole 0.0037 0.0017 0.0026 0.0118 0.0061 0.0017 0.0063 0.0011 0.001 0.0008 0.0204 0.0009 0.0023 0.0007 0.0017 0.0011 0.0022 0.0008
Vacuole Periphery 0.0013 0.0002 0.0011 0.0028 0.0056 0.0007 0.0014 0.0001 0.0003 0.0002 0.0034 0.0002 0.0015 0.0001 0.0004 0.0004 0.0006 0.0003

Sequencing Data

R1 R2
G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Post-START S/G2 Metaphase Anaphase Telophase
Gene Expression 13.6719 11.9822 11.3809 16.3533 12.5126 11.7889 13.3267 15.8633 15.1098 15.3925
Translational Efficiency 1.0453 1.0634 1.1674 0.706 0.8687 1.0819 1.1121 0.7561 0.733 0.9362

Hit Data

Dataset Hit
Protein Concentration ✘
Protein Localization ✘
Gene Expression ✘
Translational Efficiency ✘

Micrographs

Cell Count

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
1387 1250 1523 937 1661 2355 1641 206 3048 3605 3164 1143

Protein Abundance

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
Mean 729.33 895.38 1012.18 1098.03 806.03 867.28 1031.50 1142.24 771.13 877.02 1022.20 1106.00
Standard Deviation 83.92 135.35 117.78 158.71 93.11 114.96 126.76 177.92 96.89 123.14 122.90 163.23
Intensity Change Log 2 — 0.295928 0.472822 0.590274 — 0.105664 0.355838 0.502960 — 0.199177 0.412592 0.545096

Localization Score (DeepLoc)

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
Actin 0.000059 0.001196 0.000155 0.000726 0.000141 0.000224 0.001257 0.016398 0.000104 0.000561 0.000726 0.003551
Bud Neck 0.004181 0.004394 0.001326 0.004212 0.004367 0.004386 0.000926 0.018348 0.004283 0.004389 0.001119 0.006759
Bud Site 0.004148 0.013016 0.006743 0.045698 0.004460 0.010398 0.004742 0.080251 0.004318 0.011306 0.005705 0.051925
Cell Periphery 0.000138 0.000087 0.000057 0.000326 0.000083 0.000044 0.000086 0.000657 0.000108 0.000059 0.000072 0.000386
Cytoplasm 0.687939* 0.719642* 0.736231* 0.583094* 0.669204* 0.759504* 0.745616* 0.363576* 0.677729* 0.745682* 0.741099* 0.543531*
Cytoplasmic Foci 0.121105 0.109633 0.011869 0.032467 0.148162 0.077622 0.016591 0.030707 0.135849 0.088721 0.014318 0.032149
Eisosomes 0.000083 0.000030 0.000012 0.000016 0.000052 0.000020 0.000020 0.000084 0.000066 0.000024 0.000016 0.000028
Endoplasmic Reticulum 0.000396 0.000771 0.000991 0.000952 0.000890 0.000309 0.000927 0.002180 0.000665 0.000469 0.000958 0.001173
Endosome 0.004246 0.009228 0.001290 0.013491 0.008212 0.004993 0.001855 0.020644 0.006407 0.006461 0.001583 0.014780
Golgi 0.000659 0.003074 0.000086 0.014444 0.001532 0.001951 0.000161 0.096438 0.001135 0.002340 0.000125 0.029221
Lipid Particles 0.002435 0.001189 0.000550 0.000828 0.002129 0.000904 0.000640 0.001887 0.002268 0.001003 0.000597 0.001019
Mitochondria 0.000960 0.001816 0.000174 0.003221 0.001919 0.002283 0.000138 0.023070 0.001483 0.002121 0.000155 0.006798
Mitotic Spindle 0.001145 0.001453 0.004531 0.111045* 0.003063 0.012271 0.004637 0.108368* 0.002190 0.008520 0.004586 0.110563*
None 0.007265 0.002691 0.004745 0.010797 0.002114 0.002472 0.004980 0.005438 0.004458 0.002548 0.004867 0.009831
Nuclear Periphery 0.000152 0.000139 0.000541 0.002172 0.000182 0.000164 0.000490 0.000330 0.000168 0.000155 0.000515 0.001840
Nuclear Periphery Foci 0.000419 0.001511 0.002057 0.005420 0.000913 0.000387 0.003438 0.005548 0.000688 0.000777 0.002773 0.005443
Nucleolus 0.000543 0.000688 0.000096 0.000594 0.000383 0.000389 0.000135 0.001924 0.000456 0.000493 0.000116 0.000833
Nucleus 0.131109* 0.057702 0.174317* 0.067031 0.085371 0.056411 0.115179 0.033816 0.106184 0.056859 0.143645* 0.061045
Peroxisomes 0.001042 0.002750 0.000109 0.001530 0.001550 0.002271 0.000376 0.005106 0.001319 0.002437 0.000247 0.002175
Vacuole 0.031621 0.067742 0.054034 0.096416 0.064409 0.062384 0.096885 0.163912 0.049489 0.064242 0.076258 0.108580
Vacuole Periphery 0.000355 0.001248 0.000085 0.005521 0.000863 0.000610 0.000923 0.021318 0.000631 0.000832 0.000520 0.008368

Localization Changes (T-score)

R1 R2 R1 & R2
UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14 UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14 UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14
Actin -1.53 -8.71 -7.58 0.93 -4.89 -1.88 -1.26 -1.78 -1.74 -1.49 -1.77 -1.63 -2.73 -1.10 -1.54
Bud Neck -0.15 3.93 -0.80 -0.62 -6.90 -0.12 7.60 -1.02 -0.98 -2.62 -0.22 7.70 -1.30 -1.13 -7.40
Bud Site -4.76 -0.97 -7.12 -3.20 -6.54 -5.32 0.63 -4.86 -3.93 -4.92 -6.92 -0.53 -8.89 -5.57 -8.63
Cell Periphery 5.09 12.25 -0.10 -1.20 -1.69 8.72 1.68 -2.08 -2.88 -2.28 10.56 9.15 -1.10 -2.39 -2.11
Cytoplasm -2.96 3.59 20.61 21.52 17.57 -11.40 2.79 12.99 16.94 11.94 -11.11 4.38 23.95 30.57 21.12
Cytoplasmic Foci 2.26 30.10 22.57 17.79 -7.81 14.83 32.46 16.27 6.34 -5.32 13.57 43.86 31.22 20.02 -9.17
Eisosomes 12.95 17.78 15.70 3.05 -5.58 13.87 12.29 2.43 -3.97 -3.36 19.18 21.50 16.68 -2.11 -5.01
Endoplasmic Reticulum -3.01 -7.06 -3.81 -0.59 4.23 5.41 -1.15 -0.37 -3.81 0.34 2.46 -5.70 -2.05 -5.36 3.73
Endosome -5.22 6.24 -1.53 2.67 -4.78 4.02 9.42 1.30 -0.97 -3.77 -0.09 11.23 0.54 0.61 -5.61
Golgi -3.28 3.26 -1.90 0.13 -2.44 -0.91 7.35 -1.45 -1.39 -1.67 -3.23 7.82 -2.10 -1.33 -2.80
Lipid Particles 4.07 6.00 1.00 -4.89 -9.35 6.43 9.08 -3.30 -5.31 -5.64 7.22 9.92 -1.07 -8.14 -10.74
Mitochondria -1.22 2.04 0.29 1.43 -1.54 -0.35 2.43 -0.40 -0.26 -1.40 -1.01 3.03 0.52 1.53 -2.13
Mitotic Spindle -0.47 -3.36 -12.89 -12.89 -12.30 -4.29 -1.54 -6.96 -6.44 -6.82 -4.60 -3.14 -14.51 -13.51 -14.05
None 4.66 3.18 0.93 -2.88 -1.47 -1.16 -7.36 -3.06 -2.83 -1.19 4.20 -0.13 -1.58 -3.79 -1.64
Nuclear Periphery 0.52 -22.30 -5.54 -5.56 -2.91 0.44 -22.82 -9.85 -9.73 -2.48 0.35 -31.82 -6.17 -6.18 -3.14
Nuclear Periphery Foci -8.05 -9.59 -7.64 -6.24 -5.25 6.56 -10.86 -5.01 -5.34 -3.48 -1.68 -14.35 -8.74 -8.58 -5.79
Nucleolus -0.59 4.74 -1.70 -0.45 -4.01 -0.51 6.59 -0.82 -0.70 -1.88 -0.56 7.19 -2.29 -1.61 -4.32
Nucleus 17.05 -12.22 8.35 -6.61 18.79 10.16 -13.91 8.45 2.99 17.80 19.86 -18.25 6.78 -6.48 20.90
Peroxisomes -3.43 4.13 -0.79 2.63 -4.18 -1.29 3.40 -0.41 0.75 -3.38 -2.92 4.87 -0.29 2.56 -4.89
Vacuole -12.07 -16.25 -26.53 -20.95 -17.25 -0.66 -18.65 -12.61 -12.52 -7.27 -8.41 -23.81 -27.64 -25.10 -15.99
Vacuole Periphery -2.36 4.21 -1.29 0.19 -1.77 1.95 1.44 -0.98 -1.15 -1.26 -1.19 2.21 -1.28 -0.94 -1.92
Protein Complexes26S Proteasome complex37S mitochondrial small ribosomal subunit54S mitochondrial large ribosomal subunit60S cytosolic large ribosomal subunitAar2-U5 snRNPAnaphase-Promoting Complex variant 1Anaphase-Promoting Complex variant 2Anaphase-Promoting Complex variant 3Anaphase-Promoting Complex variant 4Arp2/3 complexATG1 kinase complexBLOC-1 complexCcr4-Not complexCentral kinetochore CTF19 complexCleavage and polyadenylation specificity factorcomplexCMG helicase complexCMG-Pol epsilon complexCOMPASS complexCOP9 signalosome COPI vesicle coat complexCoQ6 biosynthesis complexCore mediator complexCORVET complexCTF18-RFC complexCURI complex variant 1CURI complex variant 2CURI complex variant 3Cytoplasmic exosome complex, DIS3 variantDASH complexDNA-directed RNA Polymerase I complexDNA-directed RNA polymerase II complexDNA-directed RNA polymerase III complexDNA replication factor C complexEGO complexELG1-RFC complex Elongator holoenzyme complexEndoplasmic Reticulum Membrane ComplexEukaryotic translation initiation factor 2BcomplexEukaryotic translation initiation factor 3core complexExocystExomer complexFAR complexGeneral transcription factor complex TFIIDGeneral transcription factor complex TFIIHGET4-GET5 transmembrane domain recognition complexGID ubiquitin ligase complexGin4 complexGolgi transport complexGSE complexHMC complexHOPS complexINO80 chromatin remodeling complexLSM1-7 complexLsm1-7-Pat1 complexLSM2-7 complexLSM2-8 complexMCM complexMICOS complexMitochondrial electron transport complex IIIMitochondrial inner membrane pre-sequence translocasecomplexMitochondrial outer membrane translocase holocomplexMitochondrial proton-transporting ATP synthase complexMitochondrial pyruvate dehydrogenase complexNineTeen complexNOP8 60s ribosome pre-assembly complexNuA3 histone acetyltransferase complexNuA4 histone acetyltransferase complexNuclear condensin complexNuclear/nucleolar exosome complex, DIS3-RRP6 variantNuclear pore complexNucleolar ribonuclease MRP complexNucleolar ribonuclease P complexOligosaccharyl transferase complex variant 1Oligosaccharyl transferase complex variant 2PAF1 complexPAS complexPhosphatidylinositol 3-kinase complex IPolarisomePrefoldin complexPRP19-associated complexRad17 RFC-like complexRetromer complexRPD3L histone deacetylase complexRPD3S histone deacetylase complexRQC complexRSC complex variant RSC1RSC complex variant RSC2SAGA complexSCF-Met30 ubiquitin ligase complexSCF-Ufo1 ubiquitin ligase complexSEA complexSeptin complexSET3C histone deacetylase complexSignal recognition particleSLIK (SAGA-like) complexSmall ribosomal subunit processome, variant1Small ribosomal subunit processome, variant2Small ribosomal subunit processome, variant3SMC5-SMC6 SUMO ligase complexSm complexSpliceosomal commitment complexSPOTS complexSWI/SNF chromatin remodelling complexSwr1 chromatin remodelling complexTHO complexTranscription factor TFIIIC complexTranslocon complexTRAPPIII protein complexTRAPPII protein complexTRAPPI protein complexTREX complexU1 snRNPU2 snRNPU4 snRNPU4/U6 snRNPU4/U6.U5 tri-snRNP complexU5 snRNPU6 snRNP complexUTP-A complexUTP-B complexUTP-C complex variant 1UTP-C complex variant 2UTP-C complex variant 3Vacuolar proton translocating ATPase complex,Golgi variantVacuolar proton translocating ATPase complex,vacuole variantCellular Components••••••••••••••••actinbud neckcell peripherycytoplasmendosomeERGolgimitochondrionmixednuclear peripherynucleolusnucleusperoxisomespindle polevacuolar membranevacuole
Standard name
Human Ortholog
Description Diphthamide synthetase; catalyzes the last amidation step of diphthamide biosynthesis using ammonium and ATP; evolutionarily conserved in eukaryotes; dph6 mutants exhibit diphthine accumulation and resistance to sordarin, which is indicative of defects in diphthamide formation on EF2; green fluorescent protein (GFP)-tagged protein localizes to the cytoplasm; DPH6/YLR143W is not an essential gene
Localization
Cell Percentages cytoplasm (88%)
Cell Cycle Regulation No
Subcompartmental Group cytoplasm-9

Dph6

Dph6


Nearest neighbours ( genes)

correlation threshold

0.6

1.0

0.80


Biological Process (Gene Ontology)
Molecular Function (Gene Ontology)
Cellular Component (Gene Ontology)

Endocytosis
Temp Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Vph1-GFP)
37℃
RT
Cell Cycle Omics

CYCLoPs (Dph6-GFP)
Gene / Allele Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Sac6-tdTomato)
Gene Images
Gene Images
Images are not yet available
Images are not yet available