Standard name
Human Ortholog
Description Subunit of RNase MRP; RNase MRP processes pre-rRNA and has a role in cell cycle-regulated degradation of daughter cell-specific mRNAs; unlike most subunits, not shared between RNase MRP and nuclear RNase P
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Micrographs

ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 – – – – – –
Cell Periphery 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cytoplasm 0.06 0.12 0.11 0.14 0.3 0.28 0.3 – 0.2 0.2 0.19 0.19 0.19 0.2 0.24 0.37 0.4 0.39 0 0.05 0 0 0 0
Endoplasmic Reticulum 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0.1 0.07 0.1 0 0 0 0 0 0
Endosome 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0.12 0.11 0.14 0 0 0 0 0.05 0.1
Golgi 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Mitochondria 0.11 0.14 0 0.09 0.08 0.59 0.24 – 0.56 0.53 0.56 0.58 0 0 0 0.07 0 0.08 0 0 0 0 0.06 0
Nucleus 0.81 0.69 0.81 0.76 0.49 0.34 0.43 – 0.4 0.42 0.41 0.41 0.72 0.67 0.61 0.26 0.28 0.22 0.66 0.56 0.61 0.4 0.35 0.22
Nuclear Periphery 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0.09 0.1 0 0.08 0.08 0.09
Peroxisomes 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0.05 – 0 0 0 0 0 0 0 0 0 0 0 0.05 0.05 0.05 0.08 0.08
Vac/Vac Membrane 0.11 0.16 0.07 0 0.17 0.05 0.08 – 0.07 0.07 0.05 0.08 0.08 0.09 0.17 0.28 0.27 0.3 0.13 0.14 0.19 0.29 0.3 0.32
ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 2 1 0
Bud 0 0 0 2 0 7 4 – 7 11 6 7 0 0 0 0 0 1 3 0 2 4 5 7
Bud Neck 0 0 2 0 0 0 3 – 3 10 3 3 0 0 0 0 0 0 0 0 0 2 4 4
Bud Site 0 0 0 0 0 5 2 – 4 8 9 16 0 0 0 0 0 0 – – – – – –
Cell Periphery 0 0 0 1 0 0 0 – 1 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cytoplasm 16 30 27 32 75 78 119 – 111 105 76 112 55 69 98 83 68 45 4 14 9 10 9 9
Endoplasmic Reticulum 0 1 0 0 0 1 0 – 0 0 0 0 0 0 0 23 12 12 0 1 1 0 0 2
Endosome 1 2 1 7 8 1 9 – 3 2 1 2 5 7 17 27 18 16 4 5 5 10 18 32
Golgi 2 0 0 0 0 0 0 – 0 0 0 0 0 1 1 0 3 1 3 1 1 0 1 4
Mitochondria 30 35 3 21 19 162 97 – 313 282 219 347 6 15 2 16 3 9 0 6 4 10 24 14
Nucleus 219 170 202 179 121 94 172 – 223 220 163 249 206 234 255 58 47 25 214 159 184 105 132 73
Nuclear Periphery 1 1 0 0 0 1 2 – 3 0 0 4 0 1 1 1 0 0 1 2 2 0 3 4
Nucleolus 2 2 0 0 0 0 0 – 0 0 1 1 1 1 0 2 0 1 30 29 12 21 31 28
Peroxisomes 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 3 0 0 0 0 0 0 0 0
SpindlePole 1 0 9 5 3 1 22 – 4 10 6 9 2 3 3 0 0 4 11 14 15 12 29 27
Vac/Vac Membrane 30 39 18 7 43 15 32 – 39 38 21 48 23 32 69 63 46 34 43 40 58 76 113 104
Unique Cell Count 270 248 249 234 247 274 401 554 529 393 602 287 349 417 226 168 115 324 284 303 263 383 326
Labelled Cell Count 302 280 262 254 269 365 462 711 686 505 798 298 363 446 276 197 148 324 284 303 263 383 326


Cytoplasm, Nucleus

(Huh et al. Nature 2003 425:686)
Screen WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 AF100 AF140 AF180
Mean Cell GFP Intensity (1e-4) 4.3 4.5 5.3 4.9 5.2 3.3 4.3 – 3.8 3.9 4.0 3.9 5.4 4.8 5.5 5.9 6.3 5.9 4.7 5.1 5.3
Std Deviation (1e-4) 0.7 0.8 1.0 1.2 1.2 0.9 1.3 – 0.9 0.9 1.3 0.7 1.7 1.3 1.7 1.7 1.7 2.1 1.5 2.1 1.9
Intensity Change (Log2) – – – -0.12 -0.03 -0.68 -0.31 – -0.49 -0.42 -0.41 -0.45 0.04 -0.13 0.05 0.16 0.24 0.16 -0.16 -0.05 0.0


Localization RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3
Actin – – – – – – – – – – – – – – –
Bud – – – – – – – – – – – – 0 0 0
Bud Neck – – – – – – – – – – – – 0 0 0
Bud Site – – – – – – – – – – – – 0 0 0
Cell Periphery – – – – – – – – – – – – 0 0 0
Cyto – – – – – – – – – – – – – – –
Endoplasmic Reticulum – – – – – – – – – – – – 0 0 0
Endosome – – – – – – – – – – – – 0 0 0
Golgi – – – – – – – – – – – – 0 0 0
Mitochondria – – – – – – – – – – – – 0 0 0
Nuclear Periphery – – – – – – – – – – – – 0 0 0
Nuc – – – – – – – – – – – – – – –
Nucleolus – – – – – – – – – – – – 0 0 0
Peroxisomes – – – – – – – – – – – – 0 0 0
SpindlePole – – – – – – – – – – – – 0 0 0
Vac – – – – – – – – – – – – – – –
Cortical Patches – – – – – – – – – – – – 0 0 0
Cytoplasm – – – – – – – – – – – – 0 0 0
Nucleus – – – – – – – – – – – – 0 0 0
Vacuole – – – – – – – – – – – – 6.0 5.6 5.7

Images

Circle plot not available

Protein Concentration and Protein Localization Data

R1 R2 R3
G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase
Concentration -0.5925 -0.4141 -0.5782 -1.0401 -1.0773 -0.3801 2.3193 2.4213 1.8337 2.1338 0.6534 2.7254 3.1502 3.5706 3.2364 2.9912 3.2716 3.3068
Actin 0.0186 0.0003 0.0004 0.0002 0.0004 0.0029 0.0034 0.0002 0.0218 0.009 0.0327 0.0003 0.0269 0.0002 0.0053 0.0001 0.0022 0.0007
Bud 0.0002 0.0001 0.0001 0 0 0.0001 0.0001 0.0003 0.001 0.0015 0.0006 0.0001 0.0003 0.0001 0.0002 0.0001 0.0001 0.0001
Bud Neck 0.0014 0.0013 0.001 0.0006 0.001 0.0073 0.0015 0.0016 0.0019 0.0018 0.0024 0.0025 0.006 0.0013 0.0025 0.0007 0.0012 0.0052
Bud Periphery 0.0006 0.0002 0.0001 0 0.0001 0.0002 0.0002 0.0003 0.0005 0.0023 0.0033 0.0001 0.0005 0.0001 0.0004 0.0001 0.0003 0.0002
Bud Site 0.0006 0.0022 0.0007 0.0001 0.0002 0.0004 0.0008 0.0026 0.0028 0.0176 0.0007 0.0002 0.0037 0.0013 0.002 0.0003 0.0005 0.0004
Cell Periphery 0.0002 0.0002 0.0001 0 0 0.0001 0.0001 0.0003 0.0002 0.0004 0.0003 0 0.0002 0.0001 0.0002 0.0001 0 0.0001
Cytoplasm 0.0145 0.0367 0.0218 0.0118 0.0207 0.0173 0.1217 0.1135 0.1056 0.1603 0.0196 0.1059 0.0237 0.0275 0.0169 0.028 0.0027 0.0145
Cytoplasmic Foci 0.009 0.0061 0.0028 0.0002 0.0007 0.003 0.0252 0.0033 0.0036 0.012 0.0038 0.0018 0.0053 0.0029 0.0029 0.001 0.0016 0.0017
Eisosomes 0.0002 0.0001 0 0 0 0.0001 0.0001 0 0.0001 0.0003 0.0003 0 0.0002 0 0.0002 0 0.0001 0
Endoplasmic Reticulum 0.003 0.004 0.0014 0.002 0.002 0.0017 0.0044 0.0028 0.002 0.0093 0.0026 0.0008 0.0026 0.0018 0.0019 0.0012 0.0002 0.0011
Endosome 0.0109 0.0105 0.0054 0.0011 0.0029 0.0082 0.0173 0.005 0.0033 0.014 0.0125 0.0022 0.0071 0.002 0.0043 0.0024 0.0013 0.0031
Golgi 0.0038 0.0003 0.0002 0 0.0001 0.0015 0.0022 0.0002 0.0026 0.0013 0.0067 0.0001 0.0034 0.0001 0.0003 0 0.0025 0.0003
Lipid Particles 0.0088 0.0053 0.0026 0.0001 0.0003 0.0067 0.0077 0.0021 0.0019 0.0033 0.003 0.0004 0.0073 0.0012 0.003 0.0002 0.0196 0.0027
Mitochondria 0.0042 0.0006 0.0008 0.001 0.0008 0.0008 0.001 0.0009 0.0019 0.0077 0.0078 0.0004 0.0015 0.0004 0.0087 0.0008 0.0157 0.0004
None 0.0699 0.0849 0.0371 0.0094 0.0395 0.0474 0.0494 0.0877 0.0518 0.0662 0.1127 0.0497 0.0259 0.0776 0.0782 0.0818 0.0626 0.0306
Nuclear Periphery 0.0276 0.0365 0.0334 0.0801 0.0465 0.0201 0.0327 0.0292 0.0362 0.0457 0.027 0.0125 0.0134 0.0227 0.0154 0.0078 0.0136 0.0132
Nucleolus 0.0345 0.0679 0.0864 0.0541 0.0181 0.0702 0.0258 0.0615 0.044 0.0258 0.0227 0.0307 0.0595 0.0939 0.125 0.0896 0.101 0.0727
Nucleus 0.7466 0.6866 0.7382 0.8104 0.8352 0.746 0.6395 0.6383 0.626 0.5723 0.6009 0.7471 0.7861 0.7297 0.6924 0.7751 0.6867 0.8071
Peroxisomes 0.0105 0.0002 0.0094 0 0 0.0102 0.0065 0.0003 0.0016 0.0058 0.0006 0.0001 0.0056 0.0002 0.0005 0 0.0194 0.0003
Punctate Nuclear 0.0302 0.0492 0.0527 0.0269 0.0299 0.0541 0.0545 0.0429 0.0869 0.0199 0.1294 0.0432 0.0182 0.0347 0.0351 0.0083 0.0679 0.0441
Vacuole 0.0032 0.0054 0.0043 0.001 0.0011 0.0014 0.0039 0.0064 0.0036 0.0192 0.0056 0.0014 0.0018 0.0019 0.0039 0.0022 0.0004 0.0013
Vacuole Periphery 0.0013 0.0012 0.001 0.001 0.0005 0.0005 0.002 0.0007 0.0008 0.0046 0.0047 0.0003 0.0005 0.0003 0.0008 0.0004 0.0003 0.0002

Sequencing Data

R1 R2
G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Post-START S/G2 Metaphase Anaphase Telophase
Gene Expression 9.104 11.3938 9.6625 9.2424 10.6234 7.2792 6.583 7.22 6.088 10.7451
Translational Efficiency 1.3711 1.0894 1.1811 1.5167 1.0507 1.2338 1.7042 1.1825 1.9548 0.818

Hit Data

Dataset Hit
Protein Concentration ✘
Protein Localization ✘
Gene Expression ✘
Translational Efficiency ✘

Micrographs

Cell Count

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
2270 1582 241 2507 1332 2008 1729 1806 3602 3590 1970 4313

Protein Abundance

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
Mean 698.97 703.02 888.26 822.92 686.93 776.30 861.06 888.67 694.52 744.01 864.39 850.45
Standard Deviation 84.99 101.22 93.06 115.23 74.58 100.64 98.26 124.59 81.50 107.25 98.05 123.57
Intensity Change Log 2 — 0.008335 0.345751 0.235522 — 0.176451 0.325951 0.371485 — 0.094111 0.335971 0.304514

Localization Score (DeepLoc)

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
Actin 0.000081 0.000325 0.000485 0.000326 0.000104 0.000108 0.000255 0.000575 0.000090 0.000204 0.000283 0.000430
Bud Neck 0.004449 0.007876 0.007685 0.015730 0.001442 0.006968 0.004503 0.013705 0.003337 0.007368 0.004893 0.014882
Bud Site 0.002574 0.003236 0.001661 0.008791 0.000451 0.003422 0.000659 0.011715 0.001789 0.003341 0.000782 0.010015
Cell Periphery 0.000209 0.000160 0.000064 0.000175 0.000103 0.000168 0.000057 0.000220 0.000170 0.000165 0.000058 0.000194
Cytoplasm 0.129278* 0.146514* 0.009662 0.070859 0.103990* 0.089454 0.018461 0.115445* 0.119926* 0.114599* 0.017385 0.089529
Cytoplasmic Foci 0.028347 0.025735 0.000925 0.001548 0.027678 0.013974 0.000370 0.004363 0.028099 0.019156 0.000438 0.002727
Eisosomes 0.000037 0.000042 0.000037 0.000016 0.000042 0.000029 0.000019 0.000025 0.000039 0.000035 0.000021 0.000020
Endoplasmic Reticulum 0.001705 0.005617 0.008098 0.005085 0.001720 0.002047 0.004824 0.005876 0.001710 0.003620 0.005224 0.005416
Endosome 0.001446 0.002577 0.000622 0.001969 0.001561 0.002845 0.000389 0.003470 0.001489 0.002727 0.000417 0.002598
Golgi 0.000579 0.000621 0.000044 0.000734 0.001115 0.000680 0.000056 0.005842 0.000777 0.000654 0.000054 0.002873
Lipid Particles 0.005969 0.005296 0.000707 0.000351 0.006310 0.002371 0.000361 0.000557 0.006095 0.003660 0.000403 0.000437
Mitochondria 0.003944 0.004631 0.000655 0.004948 0.001778 0.005872 0.000609 0.008616 0.003143 0.005325 0.000614 0.006484
Mitotic Spindle 0.002436 0.001718 0.000142 0.020161 0.000582 0.006215 0.001283 0.032465 0.001750 0.004233 0.001144 0.025313
None 0.010979 0.019005 0.002039 0.005240 0.009223 0.013581 0.003348 0.009109 0.010330 0.015971 0.003188 0.006860
Nuclear Periphery 0.003509 0.003025 0.001151 0.004058 0.002693 0.002448 0.001576 0.005526 0.003207 0.002702 0.001524 0.004673
Nuclear Periphery Foci 0.000746 0.001731 0.000401 0.000791 0.001372 0.000467 0.000414 0.001357 0.000978 0.001024 0.000413 0.001028
Nucleolus 0.010410 0.016398 0.004440 0.002754 0.006638 0.011037 0.004304 0.002302 0.009015 0.013400 0.004321 0.002565
Nucleus 0.785902* 0.741804* 0.953056* 0.844141* 0.827122* 0.828844* 0.953578* 0.747932* 0.801145* 0.790488* 0.953514* 0.803855*
Peroxisomes 0.002348 0.002974 0.000214 0.000148 0.001711 0.001650 0.000096 0.000899 0.002112 0.002234 0.000110 0.000463
Vacuole 0.004515 0.009630 0.007782 0.011389 0.003698 0.006821 0.004739 0.025954 0.004213 0.008059 0.005111 0.017488
Vacuole Periphery 0.000539 0.001083 0.000130 0.000785 0.000665 0.000998 0.000099 0.004048 0.000586 0.001036 0.000103 0.002151

Localization Changes (T-score)

R1 R2 R1 & R2
UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14 UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14 UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14
Actin -7.12 -7.55 -8.51 -0.60 3.41 -0.17 -6.72 -7.88 -9.10 -3.66 -5.40 -12.34 -11.70 -7.45 -1.27
Bud Neck -4.02 -1.46 -13.34 -9.12 -3.31 -7.34 -10.96 -15.63 -7.74 -9.51 -6.83 -6.30 -19.23 -12.03 -12.79
Bud Site -0.75 0.80 -3.84 -3.24 -4.45 -4.63 -2.22 -7.73 -4.43 -7.07 -2.61 1.30 -7.25 -5.24 -8.62
Cell Periphery 2.00 5.71 1.96 0.86 -1.36 -3.19 6.25 -1.52 0.79 -3.28 0.42 7.47 1.39 1.15 -2.92
Cytoplasm -2.62 27.14 14.17 13.94 -15.78 2.53 18.66 -0.13 -2.78 -20.08 1.29 32.83 10.46 8.56 -24.50
Cytoplasmic Foci 1.08 14.91 15.36 12.84 -0.34 5.73 12.35 11.31 9.32 -4.86 5.36 20.10 19.01 16.02 -4.66
Eisosomes -2.20 -1.61 9.47 9.94 4.47 4.21 5.23 2.52 -3.11 -4.91 2.03 5.03 7.95 6.14 1.42
Endoplasmic Reticulum -9.55 -9.91 -17.22 -0.85 4.16 -1.54 -16.35 -17.68 -16.62 -4.20 -8.96 -20.29 -24.70 -11.60 -0.67
Endosome -3.44 2.43 0.19 3.30 -1.85 -1.95 2.77 -1.08 1.20 -5.91 -3.71 4.83 -0.75 3.14 -6.08
Golgi -0.22 3.63 0.80 1.17 -2.35 0.85 2.06 -1.26 -2.08 -2.90 0.60 3.43 -1.04 -1.59 -3.41
Lipid Particles 0.88 9.74 11.13 8.22 3.13 4.93 7.33 7.00 7.86 -3.18 4.82 12.76 12.84 10.83 0.11
Mitochondria -1.07 6.73 0.17 1.08 -4.95 -4.04 2.77 -2.93 0.85 -4.30 -3.35 7.24 -1.66 1.45 -6.50
Mitotic Spindle 0.93 2.96 -5.90 -6.74 -7.77 -3.89 -1.49 -8.73 -6.08 -8.09 -2.37 0.26 -10.11 -8.37 -10.12
None -4.57 8.76 6.07 9.67 -5.48 -2.46 4.85 1.61 4.29 -4.53 -4.50 8.60 5.61 9.73 -4.89
Nuclear Periphery 1.23 6.43 -3.67 -5.61 -14.15 0.59 1.65 -5.33 -6.16 -7.19 1.73 4.82 -6.35 -8.53 -12.04
Nuclear Periphery Foci -3.36 0.31 0.12 3.29 -0.20 2.28 1.17 0.48 -3.85 -1.17 -0.22 0.65 0.36 0.69 -0.42
Nucleolus -3.92 3.71 7.53 10.50 1.27 -3.09 1.46 5.73 8.57 5.01 -4.18 4.32 9.46 13.09 4.37
Nucleus 4.89 -17.74 -9.22 -12.78 11.14 -0.54 -16.88 7.65 8.68 26.60 1.50 -29.66 -1.67 -3.13 29.02
Peroxisomes -1.15 6.76 7.14 7.41 2.22 0.49 5.40 3.96 4.81 -2.03 -0.14 8.83 8.02 9.29 -1.76
Vacuole -5.09 -2.86 -10.81 -5.47 -1.13 -3.87 -5.11 -17.84 -16.30 -15.11 -5.91 -5.34 -20.41 -16.64 -13.94
Vacuole Periphery -3.66 4.08 0.13 3.63 -3.55 -0.96 1.78 -2.07 -1.67 -3.84 -2.73 3.62 -1.98 -0.03 -4.86
Standard name
Human Ortholog
Description Subunit of RNase MRP; RNase MRP processes pre-rRNA and has a role in cell cycle-regulated degradation of daughter cell-specific mRNAs; unlike most subunits, not shared between RNase MRP and nuclear RNase P
Localization
Cell Percentages nucleus (65%)
Cell Cycle Regulation No
Subcompartmental Group nucleus-3

Rmp1

Rmp1


Nearest neighbours ( genes)

correlation threshold

0.6

1.0

0.80


Biological Process (Gene Ontology)
Molecular Function (Gene Ontology)
Cellular Component (Gene Ontology)

Endocytosis
Temp Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Vph1-GFP)
37℃
RT
Cell Cycle Omics

CYCLoPs (Rmp1-GFP)
Gene / Allele Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Sac6-tdTomato)
Gene Images
Gene Images
Images are not yet available
Images are not yet available